Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0014032

Physical Location & Seq

Genomic Coordinates Forward (+)

19700333 .. 19703788

Gene Structure 3456 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g17250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1185 bp

ATGATTTGCCAGATTAAAAAAAGCAGTGTCCAGTACTCAGAGCGAGCAGCTGGACTTTCCTCAAATCAACCGACTCCCTTCAGAAAGTTTGAAACGATGGCGAGCTCGAAGACTCTGATCGCTTTCGGAGCCCTAGCCCTGCTCTTTGCCTCGTCGGCCTTCGCAGACGACGTCGTTGTGCTCACGGAGGACAACTTCGAGAAGGAGGTCGGTCAAGATCGCGCCGCTCTCGTCGAGTTCTACGCTCCCTGGTGTGGTCACTGTAAGAAGCTTGCACCGGAGTATGAGAAGCTAGGTTCAAGTTTTAAGAAAGCAAAATCGATTTTAATCGCAAAGATTGATTGTGATGAGCACAAGGGCGTTTGTGGCAAGTTTGGAGTTTCCGGCTACCCCACAATTCAATGGTTTCCTAAAGGGTCCCTAGAACCCAAAAAGTATGAAGGTGCCCGTACTGCTGAAGCTCTTGCTGAGTTTGTGAATAAGGAAGGAGGGACCAATGTGAAGATTGCTGCAGACCCATCCCATGTTGTGGTACTTACACAGGATAACTTTAATGAGGTTGTCCTGGATGAAACAAAAGATGTGCTGGTGGAGTTCTATGCACCATGGTGTGGACATTGCAAATCCCTTGCTCCTACTTATGAGAAGGTTGCCTCAGCATTTAAGTTGGAGGACGGCGTAGTGATTGCTAATCTTGATGCTGACAAATACAAGGATTTAGCTGAAAAGTACGGTGTTAGTGGATATCCCACATTGAAATTCTTCCCAAAGAACAAGAAGGAGGGTGAAGAGTATGGTGGCGGCAGAGATTTGGAGGATTTTGTAGATTTCATTAATGAGAAATCTGGCACCAGCAGAGACGCAAAAGGACAACTTACTTCCAAAGCTGGCATTGTTGAAAATTTGGACGAATTAGTAAAGGAGTTTGTGAAATCTAGCAACGATGAGAAGAAGGCAATATTTTCCAAGATAGAAGAGGAAGTTGGGAAACTTGAGGGTTCGGCTGCAAGATACGGTAACATCTACTTGAAAGCCGCCAAGAAGTCTTTGGAGAAAGGTGCTGATTATGCGAAGAACGAAATTCAGCGGCTTGAGCGCATACTTGAAAAGTCTGTCAACCCAACAAAGGCCGATGAGTTCACTCTCAAGAAGAACATTCTATACACCTTTGCTTCATCTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0006457 GO:0006950 GO:0007275 GO:0008150 GO:0009505 GO:0009553 GO:0009566 GO:0009567 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0012505 GO:0016020 GO:0016853 GO:0016860 GO:0016864 GO:0019953 GO:0022414 GO:0030312 GO:0031090 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046686 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0050896 GO:0051704 GO:0051716 GO:0061458 GO:0071944 GO:0098588 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

ERp29 Thioredoxin

Protein Analysis

395

Amino Acids

43.5

Weight (kDa)

6.03

Isoelectric Point (pI)

25.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	58 - 160	9e-33	Thioredoxin
Thioredoxin_2	PF13098	73 - 157	1.3e-06	Thioredoxin-like domain
OST3_OST6	PF04756	85 - 174	8.2e-09	OST3 / OST6 family, transporter family
OST3_OST6	PF04756	175 - 283	4.3e-06	OST3 / OST6 family, transporter family
Thioredoxin	PF00085	176 - 279	7.2e-33	Thioredoxin
Thioredoxin_2	PF13098	191 - 275	1.2e-06	Thioredoxin-like domain
ERp29	PF07749	297 - 390	1.3e-25	Endoplasmic reticulum protein ERp29, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47470 AT2G47470 AT2G47470 AT2G47470
fragaria_vesca	FvH4_6g07230
malus_domestica	MD04G1195300.v1.1 MD12G1207400.v1.1
prunus_persica	Prupe.6G315000_v2.0.a1
pyrus_communis	pycom04g17250
rosa_chinensis	RchiOBHm_Chr3g0456861
rosa_laevigata	RLG00000025273
rosa_multiflora	Rmu_sc0000133.1_g000018
rosa_roxburghii	Rroxscaffold_6G00422430
rosa_rugosa	Rorug03G0016400
rosa_samantha	Rh3BG078600 Rh3CG078400
rosa_wichuraiana	Rw3G006590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	174
AccB1I	GGYRCC	2 cut(s)	443, 848
AccB7I	CCANNNNNTGG	2 cut(s)	529, 611
AccBSI	CCGCTC	1 cut(s)	227
AccII	CGCG	1 cut(s)	222
AciI	CCGC	4 cut(s)	225, 801, 1035, 1087
AcsI	RAATTY	3 cut(s)	758, 901, 1080
AcuI	CTGAAG	2 cut(s)	64, 477
AcyI	GRCGYC	1 cut(s)	171
AfaI	GTAC	4 cut(s)	35, 451, 534, 731
AfiI	CCNNNNNNNGG	4 cut(s)	254, 529, 611, 1126
AgsI	TTSAA	7 cut(s)	92, 300, 401, 757, 899, 1030, 1106
AjnI	CCWGG	2 cut(s)	248, 564
AjuI	GAANNNNNNNTTGG	2 cut(s)	966, 998
AleI	CACNNNNGTG	1 cut(s)	607
AluBI	AGCT	7 cut(s)	50, 105, 271, 292, 461, 722, 887
AluI	AGCT	7 cut(s)	50, 105, 271, 292, 461, 722, 887
Alw21I	GWGCWC	3 cut(s)	107, 183, 354
Alw26I	GTCTC	1 cut(s)	852
AoxI	GGCC	2 cut(s)	156, 1128
ApeKI	GCWGC	3 cut(s)	47, 509, 1004
ApoI	RAATTY	3 cut(s)	758, 901, 1080
AseI	ATTAAT	1 cut(s)	834
Asp700I	GAANNNNTTC	1 cut(s)	761
AspLEI	GCGC	2 cut(s)	224, 1098
AspS9I	GGNCC	2 cut(s)	417, 492
AsuHPI	GGTGA	1 cut(s)	797
AvaII	GGWCC	2 cut(s)	417, 492
BaeGI	GKGCMC	1 cut(s)	448
BanI	GGYRCC	2 cut(s)	443, 848
BanII	GRGCYC	2 cut(s)	107, 133
BbsI	GAAGAC	1 cut(s)	116
Bbv12I	GWGCWC	3 cut(s)	107, 183, 354
BbvCI	CCTCAGC	1 cut(s)	655
BbvI	GCAGC	3 cut(s)	59, 496, 991
BccI	CCATC	2 cut(s)	91, 526
BceAI	ACGGC	1 cut(s)	691
BciT130I	CCWGG	2 cut(s)	250, 566
BcoDI	GTCTC	1 cut(s)	852
BfaI	CTAG	4 cut(s)	134, 293, 422, 936
BfmI	CTRYAG	1 cut(s)	510
BglI	GCCNNNNNGGC	1 cut(s)	155
BisI	GCNGC	7 cut(s)	48, 225, 510, 802, 1005, 1035, 1088
BlsI	GCNGC	7 cut(s)	49, 226, 511, 803, 1006, 1036, 1089
BmcAI	AGTACT	1 cut(s)	35
Bme1390I	CCNGG	2 cut(s)	250, 566
Bme18I	GGWCC	2 cut(s)	417, 492
BmgT120I	GGNCC	2 cut(s)	417, 492
BmiI	GGNNCC	6 cut(s)	130, 418, 419, 445, 493, 850
BmrFI	CCNGG	2 cut(s)	250, 566
BmsI	GCATC	1 cut(s)	688
BpiI	GAAGAC	1 cut(s)	116
Bpu10I	CCTNAGC	1 cut(s)	655
BpuEI	CTTGAG	3 cut(s)	1013, 1112, 1130
Bsa29I	ATCGAT	1 cut(s)	320
BsaBI	GATNNNNATC	1 cut(s)	326
BsaHI	GRCGYC	1 cut(s)	171
BsaJI	CCNNGG	2 cut(s)	248, 605
BsaWI	WCCGGW	1 cut(s)	277
Bsc4I	CCNNNNNNNGG	4 cut(s)	254, 529, 611, 1126
Bse1I	ACTGG	1 cut(s)	31
Bse3DI	GCAATG	1 cut(s)	616
Bse8I	GATNNNNATC	1 cut(s)	326
BseBI	CCWGG	2 cut(s)	250, 566
BseCI	ATCGAT	1 cut(s)	320
BseDI	CCNNGG	2 cut(s)	248, 605
BseGI	GGATG	2 cut(s)	518, 574
BseJI	GATNNNNATC	1 cut(s)	326
BseLI	CCNNNNNNNGG	4 cut(s)	254, 529, 611, 1126
BseMI	GCAATG	1 cut(s)	616
BseMII	CTCAG	3 cut(s)	51, 459, 669
BseNI	ACTGG	1 cut(s)	31
BseSI	GKGCMC	1 cut(s)	448
BseXI	GCAGC	3 cut(s)	59, 496, 991
Bsh1236I	CGCG	1 cut(s)	222
BshFI	GGCC	2 cut(s)	158, 1130
BshNI	GGYRCC	2 cut(s)	443, 848
BshVI	ATCGAT	1 cut(s)	320
BsiHKAI	GWGCWC	3 cut(s)	107, 183, 354
BsiSI	CCGG	2 cut(s)	278, 384
BslFI	GGGAC	2 cut(s)	403, 505
BslI	CCNNNNNNNGG	4 cut(s)	254, 529, 611, 1126
BsmAI	GTCTC	1 cut(s)	852
BsmBI	CGTCTC	1 cut(s)	852
BsmFI	GGGAC	2 cut(s)	403, 505
BsnI	GGCC	2 cut(s)	158, 1130
Bsp1286I	GDGCHC	5 cut(s)	107, 133, 183, 354, 448
Bsp143I	GATC	2 cut(s)	117, 217
Bsp19I	CCATGG	1 cut(s)	605
BspACI	CCGC	4 cut(s)	225, 801, 1035, 1087
BspANI	GGCC	2 cut(s)	158, 1130
BspCNI	CTCAG	3 cut(s)	50, 460, 668
BspDI	ATCGAT	1 cut(s)	320
BspFNI	CGCG	1 cut(s)	222
BspHI	TCATGA	1 cut(s)	1181
BspLI	GGNNCC	6 cut(s)	130, 418, 419, 445, 493, 850
BspMAI	CTGCAG	1 cut(s)	514
BspT107I	GGYRCC	2 cut(s)	443, 848
BsrBI	CCGCTC	1 cut(s)	227
BsrDI	GCAATG	1 cut(s)	616
BsrI	ACTGG	1 cut(s)	31
BssECI	CCNNGG	2 cut(s)	248, 605
BssMI	GATC	2 cut(s)	117, 217
BssNI	GRCGYC	1 cut(s)	171
BssT1I	CCWWGG	1 cut(s)	605
Bst2UI	CCWGG	2 cut(s)	250, 566
Bst4CI	ACNGT	3 cut(s)	263, 734, 1016
Bst6I	CTCTTC	2 cut(s)	783, 969
BstACI	GRCGYC	1 cut(s)	171
BstC8I	GCNNGC	4 cut(s)	45, 103, 273, 889
BstDEI	CTNAG	3 cut(s)	37, 468, 655
BstDSI	CCRYGG	1 cut(s)	605
BstF5I	GGATG	2 cut(s)	518, 574
BstFNI	CGCG	1 cut(s)	222
BstHHI	GCGC	2 cut(s)	224, 1098
BstKTI	GATC	2 cut(s)	120, 220
BstMAI	GTCTC	1 cut(s)	852
BstMBI	GATC	2 cut(s)	117, 217
BstMWI	GCNNNNNNNGC	6 cut(s)	128, 155, 366, 452, 1067, 1093
BstNI	CCWGG	2 cut(s)	250, 566
BstSCI	CCNGG	2 cut(s)	248, 564
BstSFI	CTRYAG	1 cut(s)	510
BstSLI	GKGCMC	1 cut(s)	448
BstUI	CGCG	1 cut(s)	222
BstV1I	GCAGC	3 cut(s)	59, 496, 991
BstV2I	GAAGAC	1 cut(s)	116
Bsu15I	ATCGAT	1 cut(s)	320
BsuRI	GGCC	2 cut(s)	158, 1130
BsuTUI	ATCGAT	1 cut(s)	320
BtgI	CCRYGG	1 cut(s)	605
BtsCI	GGATG	2 cut(s)	518, 574
BtsI	GCAGTG	1 cut(s)	31
BtsIMutI	CAGTG	2 cut(s)	31, 259
Cac8I	GCNNGC	4 cut(s)	45, 103, 273, 889
CciI	TCATGA	1 cut(s)	1181
CfoI	GCGC	2 cut(s)	224, 1098
Cfr13I	GGNCC	2 cut(s)	417, 492
ClaI	ATCGAT	1 cut(s)	320
CseI	GACGC	1 cut(s)	869
Csp6I	GTAC	4 cut(s)	34, 450, 533, 730
CviAII	CATG	3 cut(s)	524, 606, 1182
CviQI	GTAC	4 cut(s)	34, 450, 533, 730
DdeI	CTNAG	3 cut(s)	37, 468, 655
DpnI	GATC	2 cut(s)	119, 219
DpnII	GATC	2 cut(s)	117, 217
Eam1104I	CTCTTC	2 cut(s)	783, 969
EarI	CTCTTC	2 cut(s)	783, 969
Ecl136II	GAGCTC	1 cut(s)	105
Eco130I	CCWWGG	1 cut(s)	605
Eco24I	GRGCYC	2 cut(s)	107, 133
Eco32I	GATATC	1 cut(s)	746
Eco47I	GGWCC	2 cut(s)	417, 492
Eco53kI	GAGCTC	1 cut(s)	105
Eco57I	CTGAAG	2 cut(s)	64, 477
EcoICRI	GAGCTC	1 cut(s)	105
EcoO109I	RGGNCCY	1 cut(s)	417
EcoRII	CCWGG	2 cut(s)	248, 564
EcoRV	GATATC	1 cut(s)	746
EcoT14I	CCWWGG	1 cut(s)	605
EcoT38I	GRGCYC	2 cut(s)	107, 133
ErhI	CCWWGG	1 cut(s)	605
Esp3I	CGTCTC	1 cut(s)	852
FaeI	CATG	3 cut(s)	527, 609, 1185
FalI	AAGNNNNNCTT	2 cut(s)	858, 890
FaqI	GGGAC	2 cut(s)	403, 505
FatI	CATG	3 cut(s)	523, 605, 1181
Fnu4HI	GCNGC	7 cut(s)	48, 225, 510, 802, 1005, 1035, 1088
FokI	GGATG	2 cut(s)	505, 581
FriOI	GRGCYC	2 cut(s)	107, 133
Fsp4HI	GCNGC	7 cut(s)	48, 225, 510, 802, 1005, 1035, 1088
FspBI	CTAG	4 cut(s)	134, 293, 422, 936
GlaI	GCGC	2 cut(s)	223, 1097
GluI	GCNGC	7 cut(s)	48, 225, 510, 802, 1005, 1035, 1088
HaeIII	GGCC	2 cut(s)	158, 1130
HapII	CCGG	2 cut(s)	278, 384
HgaI	GACGC	1 cut(s)	869
HhaI	GCGC	2 cut(s)	224, 1098
Hin1I	GRCGYC	1 cut(s)	171
Hin1II	CATG	3 cut(s)	527, 609, 1185
Hin6I	GCGC	2 cut(s)	222, 1096
HinP1I	GCGC	2 cut(s)	222, 1096
HincII	GTYRAC	1 cut(s)	1117
HindII	GTYRAC	1 cut(s)	1117
HindIII	AAGCTT	1 cut(s)	269
HinfI	GANTC	2 cut(s)	73, 112
HpaII	CCGG	2 cut(s)	278, 384
HphI	GGTGA	1 cut(s)	797
Hpy166II	GTNNAC	3 cut(s)	614, 1117, 1140
Hpy188I	TCNGA	4 cut(s)	40, 83, 117, 128
Hpy188III	TCNNGA	5 cut(s)	199, 215, 695, 1147, 1182
Hpy8I	GTNNAC	3 cut(s)	614, 1117, 1140
Hpy99I	CGWCG	4 cut(s)	157, 173, 176, 236
HpyAV	CCTTC	8 cut(s)	88, 169, 196, 434, 479, 640, 772, 946
HpyCH4III	ACNGT	3 cut(s)	263, 734, 1016
HpyCH4IV	ACGT	1 cut(s)	171
HpyCH4V	TGCA	5 cut(s)	275, 512, 602, 621, 1007
HpyF10VI	GCNNNNNNNGC	6 cut(s)	128, 155, 366, 452, 1067, 1093
HpyF3I	CTNAG	3 cut(s)	37, 468, 655
HpySE526I	ACGT	1 cut(s)	171
Hsp92I	GRCGYC	1 cut(s)	171
Hsp92II	CATG	3 cut(s)	527, 609, 1185
HspAI	GCGC	2 cut(s)	222, 1096
KflI	GGGWCCC	1 cut(s)	417
Kzo9I	GATC	2 cut(s)	117, 217
LmnI	GCTCC	3 cut(s)	128, 250, 637
Lsp1109I	GCAGC	3 cut(s)	59, 496, 991
LweI	GCATC	1 cut(s)	688
MaeI	CTAG	4 cut(s)	134, 293, 422, 936
MaeII	ACGT	1 cut(s)	171
MaeIII	GTNAC	2 cut(s)	257, 1016
MalI	GATC	2 cut(s)	119, 219
MbiI	CCGCTC	1 cut(s)	227
MboI	GATC	2 cut(s)	117, 217
MboII	GAAGA	9 cut(s)	121, 514, 754, 800, 961, 986, 1084, 1162, 1170
MhlI	GDGCHC	5 cut(s)	107, 133, 183, 354, 448
MluCI	AATT	5 cut(s)	396, 758, 901, 911, 1080
MlyI	GAGTC	2 cut(s)	67, 106
MmeI	TCCRAC	1 cut(s)	648
MroXI	GAANNNNTTC	1 cut(s)	761
MseI	TTAA	6 cut(s)	15, 306, 326, 552, 663, 834
MslI	CAYNNNNRTG	2 cut(s)	607, 1180
MspA1I	CMGCKG	2 cut(s)	50, 1087
MspI	CCGG	2 cut(s)	278, 384
MspR9I	CCNGG	2 cut(s)	250, 566
MvaI	CCWGG	2 cut(s)	250, 566
MvnI	CGCG	1 cut(s)	222
MwoI	GCNNNNNNNGC	6 cut(s)	128, 155, 366, 452, 1067, 1093
NcoI	CCATGG	1 cut(s)	605
NdeII	GATC	2 cut(s)	117, 217
NlaIII	CATG	3 cut(s)	527, 609, 1185
NlaIV	GGNNCC	6 cut(s)	130, 418, 419, 445, 493, 850
NmuCI	GTSAC	1 cut(s)	257
OliI	CACNNNNGTG	1 cut(s)	607
PagI	TCATGA	1 cut(s)	1181
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PdmI	GAANNNNTTC	1 cut(s)	761
PflFI	GACNNNGTC	1 cut(s)	170
PflMI	CCANNNNNTGG	2 cut(s)	529, 611
PfoI	TCCNGGA	1 cut(s)	564
PkrI	GCNGC	7 cut(s)	49, 226, 511, 803, 1006, 1036, 1089
PleI	GAGTC	2 cut(s)	67, 106
PpsI	GAGTC	2 cut(s)	67, 106
PpuMI	RGGWCCY	1 cut(s)	417
PshBI	ATTAAT	1 cut(s)	834
Psp124BI	GAGCTC	1 cut(s)	107
Psp5II	RGGWCCY	1 cut(s)	417
Psp6I	CCWGG	2 cut(s)	248, 564
PspGI	CCWGG	2 cut(s)	248, 564
PspN4I	GGNNCC	6 cut(s)	130, 418, 419, 445, 493, 850
PspPI	GGNCC	2 cut(s)	417, 492
PspPPI	RGGWCCY	1 cut(s)	417
PsrI	GAACNNNNNNTAC	2 cut(s)	1145, 1177
PstI	CTGCAG	1 cut(s)	514
PsyI	GACNNNGTC	1 cut(s)	170
PvuII	CAGCTG	1 cut(s)	50
RsaI	GTAC	4 cut(s)	35, 451, 534, 731
RsaNI	GTAC	4 cut(s)	34, 450, 533, 730
RseI	CAYNNNNRTG	2 cut(s)	607, 1180
SacI	GAGCTC	1 cut(s)	107
SaqAI	TTAA	6 cut(s)	15, 306, 326, 552, 663, 834
SatI	GCNGC	7 cut(s)	48, 225, 510, 802, 1005, 1035, 1088
Sau3AI	GATC	2 cut(s)	117, 217
Sau96I	GGNCC	2 cut(s)	417, 492
ScaI	AGTACT	1 cut(s)	35
SchI	GAGTC	2 cut(s)	67, 106
ScrFI	CCNGG	2 cut(s)	250, 566
SduI	GDGCHC	5 cut(s)	107, 133, 183, 354, 448
SfaNI	GCATC	1 cut(s)	688
SfcI	CTRYAG	1 cut(s)	510
SinI	GGWCC	2 cut(s)	417, 492
SmiMI	CAYNNNNRTG	2 cut(s)	607, 1180
SmlI	CTYRAG	3 cut(s)	992, 1091, 1145
SmoI	CTYRAG	3 cut(s)	992, 1091, 1145
Sse9I	AATT	5 cut(s)	396, 758, 901, 911, 1080
SsiI	CCGC	4 cut(s)	225, 801, 1035, 1087
SspI	AATATT	1 cut(s)	960
SspMI	CTAG	4 cut(s)	134, 293, 422, 936
SstI	GAGCTC	1 cut(s)	107
StyD4I	CCNGG	2 cut(s)	248, 564
StyI	CCWWGG	1 cut(s)	605
TaaI	ACNGT	3 cut(s)	263, 734, 1016
TaiI	ACGT	1 cut(s)	174
TaqI	TCGA	4 cut(s)	107, 198, 234, 320
TaqII	GACCGA	1 cut(s)	200
TasI	AATT	5 cut(s)	396, 758, 901, 911, 1080
TatI	WGTACW	1 cut(s)	33
TauI	GCSGC	4 cut(s)	227, 804, 1037, 1090
Tru1I	TTAA	6 cut(s)	15, 306, 326, 552, 663, 834
Tru9I	TTAA	6 cut(s)	15, 306, 326, 552, 663, 834
TscAI	CASTG	2 cut(s)	31, 266
TseFI	GTSAC	1 cut(s)	257
TseI	GCWGC	3 cut(s)	47, 509, 1004
Tsp45I	GTSAC	1 cut(s)	257
TspDTI	ATGAA	5 cut(s)	453, 585, 820, 1164, 1170
TspGWI	ACGGA	1 cut(s)	200
TspRI	CASTG	2 cut(s)	31, 266
Tth111I	GACNNNGTC	1 cut(s)	170
Van91I	CCANNNNNTGG	2 cut(s)	529, 611
VpaK11BI	GGWCC	2 cut(s)	417, 492
VspI	ATTAAT	1 cut(s)	834
XapI	RAATTY	3 cut(s)	758, 901, 1080
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1045
XmnI	GAANNNNTTC	1 cut(s)	761
XspI	CTAG	4 cut(s)	134, 293, 422, 936
ZraI	GACGTC	1 cut(s)	172
ZrmI	AGTACT	1 cut(s)	35

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.