Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0002353

Physical Location & Seq

Genomic Coordinates Forward (+)

1300028 .. 1304226

Gene Structure 4199 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0016400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGAGGTATGAGTTGGCGGAAGAGTGCGCAGATGACGATGATGCAAAGCTAGGAATTTCTGAGTTAAGGAGTGCTGTTCTTCAGGAACTGAAAATGCATAATTCATTCGTGCAGGAATGGGGTTTAGATGCTAAGGACATCTCTATCAGCTCTGCAGCAGTAAAGTACACAGAATTCTTATTGGCAACAGCCTCTGGTAAAGTTGAAGGAGTAAAAGGCCCTGGTAAACTTGCGACTCCATTTGAAAGAACCAAAGTTGCTGCTTACACCCTTGGTGCCATGACTCCTTGCATGAGATTGTATGCCTTTATAGGTAAGGAGTTCAAGGCACTTCTAGATCCCAAAGACGGCACTCATCCATACAAGAAGTGGATCGACAATTATTCTTCCGAAAGTTTTCAGGCATCAGCTGTGCAAACTGAAGAGTTGCTGGATAAGCTAAGTGTCTCTTTGACTGGCGAGGAGCTTGACATCATTGAAAAGCTTTATCATCAAGCCATGAAACTTGAGATAGAGTTCTTTTCAGCCCAGTCACTTCTTCAACCAACTGTAGTTCCTCTTATCAAAGATCATGACTCTGCAAAAGATCACCTCATGATATTTTCAGATTTTGATTTGACTTGTACTGTTGTTGATTCCTCTGCCATTTTGGCTGAAATTGCAATAGTAACTGCCCCAAAACCAGATCAAAATCAATCTGAAGATCAGATTACGCGGATGTCATCGGCTGATCTCAGAAATACATGGTGCCTTCTTTCCAAGCAGTACACAGAAGAGTACGAGCAATGCATAGAAAGCATTATTCCTACTAAAAGAGCGGAGTTTGTCTATAACAATCTGCATAAAGCACTCGAGCAACTTTCAGAGTTCGAGAAAAAGGCAAACAATAGAGTGATAGAGTCAGGGGTTCTGAAGGGTCTTAATATTGAAGATATAAAAAGAGCTGGTGAACGTCTCATTCTTCAAGATGGTTGTATTGATTTCTTCAAGAAAATTATCAAAAGTGATAACTTGAACGCAAATGTTCATGTTCTTTCATATTGTTGGTGTGGTGATCTTATTAGGTCAGCTTTTTCATCAGGGGGTTTACATGAACCAAATGTTCATGCAAATGAGTTCACATTTGAAGAATCTATTTCCACTGGTAAAATTGTTAAGAAGGTGGAGTCTCCTATTGACAAGGTTCAAGCTTTCAGAGATATTATAAAAAGTTGTCAAAGTGACAGGAAGAACTTGACTATTTACATTGGAGACTCGGTGGGTGACTTACTATGCCTCCTGGAGGCAGATATAGGAATTGTCATAGGGTCAAGCTCAAGCCTGAGGAGAGTGGGGACTCAATTCGGTGTTTCTTTTGTTCCACTGTTTCCTGGTTTAGTTAAGAAACAAAAAGAATGCGTTGAAGGAAGGTCTTCTAATTGGAAAGGGTTAACTGGCATTCTTTATACAGCATCTAGTTGGGCTGAAATACATGCCTTCATTTTGGGGTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0006457 GO:0006950 GO:0007275 GO:0008150 GO:0009505 GO:0009553 GO:0009566 GO:0009567 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0012505 GO:0016020 GO:0016853 GO:0016860 GO:0016864 GO:0019953 GO:0022414 GO:0030312 GO:0031090 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046686 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0050896 GO:0051704 GO:0051716 GO:0061458 GO:0071944 GO:0098588 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

ERp29 Thioredoxin

Protein Analysis

497

Amino Acids

55.36

Weight (kDa)

5.24

Isoelectric Point (pI)

45.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TENA_THI-4	PF03070	86 - 176	3.5e-09	TENA/THI-4/PQQC family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62630
fragaria_vesca	FvH4_6g07220 FvH4_6g07220 FvH4_6g07220
malus_domestica	MD04G1195400.v1.1 MD12G1207500.v1.1
prunus_persica	Prupe.6G315100_v2.0.a1
pyrus_communis	pycom04g17260 pycom12g19510 pycom15g32780
rosa_chinensis	RchiOBHm_Chr1g0333511 RchiOBHm_Chr1g0352421 RchiOBHm_Chr2g0129671 RchiOBHm_Chr3g0456851 RchiOBHm_Chr5g0038721 RchiOBHm_Chr6g0249361
rosa_laevigata	RLG00000023272 RLG00000025274
rosa_multiflora	Rmu_co8019462.1_g000001 Rmu_sc0000133.1_g000019 Rmu_sc0000741.1_g000014 Rmu_sc0001016.1_g000061 Rmu_sc0001890.1_g000010 Rmu_sc0002095.1_g000008 Rmu_sc0002955.1_g000021 Rmu_sc0005500.1_g000015 Rmu_sc0010617.1_g000006 Rmu_sc0020548.1_g000004 Rmu_sc0020548.1_g000005 Rmu_sc0033338.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223580 Rroxscaffold_6G00422440
rosa_rugosa	Rorug03G0016400
rosa_samantha	Rh1AG132800 Rh1AG157400 Rh1CG147200 Rh3AG076700 Rh3BG078500 Rh3CG078300 Rh3DG080300 Rh4DG243900
rosa_wichuraiana	Rw3G006580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1205
Acc16I	TGCGCA	1 cut(s)	28
AccB1I	GGYRCC	2 cut(s)	275, 747
AccBSI	CCGCTC	1 cut(s)	818
AccII	CGCG	1 cut(s)	715
AciI	CCGC	3 cut(s)	17, 715, 818
AclWI	GGATC	2 cut(s)	332, 380
AcsI	RAATTY	2 cut(s)	54, 173
AcuI	CTGAAG	4 cut(s)	65, 441, 720, 932
AfaI	GTAC	4 cut(s)	167, 625, 767, 779
AfiI	CCNNNNNNNGG	2 cut(s)	347, 1282
AjnI	CCWGG	3 cut(s)	220, 1278, 1369
Alw26I	GTCTC	4 cut(s)	451, 959, 1173, 1245
AlwI	GGATC	2 cut(s)	332, 380
AlwNI	CAGNNNCTG	2 cut(s)	88, 194
Ama87I	CYCGRG	1 cut(s)	851
AoxI	GGCC	1 cut(s)	217
ApeKI	GCWGC	2 cut(s)	155, 260
ApoI	RAATTY	2 cut(s)	54, 173
Asp700I	GAANNNNTTC	2 cut(s)	396, 1411
AspLEI	GCGC	1 cut(s)	29
AspS9I	GGNCC	1 cut(s)	218
AsuHPI	GGTGA	4 cut(s)	581, 959, 1064, 1274
AvaI	CYCGRG	1 cut(s)	851
AxyI	CCTNAGG	1 cut(s)	1322
BanI	GGYRCC	2 cut(s)	275, 747
BbsI	GAAGAC	1 cut(s)	1404
BbvI	GCAGC	2 cut(s)	167, 247
BccI	CCATC	1 cut(s)	962
BceAI	ACGGC	1 cut(s)	364
BciT130I	CCWGG	3 cut(s)	222, 1280, 1371
BcoDI	GTCTC	4 cut(s)	451, 959, 1173, 1245
BfaI	CTAG	3 cut(s)	50, 335, 1455
BfmI	CTRYAG	2 cut(s)	153, 549
BglI	GCCNNNNNGGC	1 cut(s)	650
BisI	GCNGC	2 cut(s)	156, 261
BlsI	GCNGC	2 cut(s)	157, 262
Bme1390I	CCNGG	3 cut(s)	222, 1280, 1371
BmeT110I	CYCGRG	1 cut(s)	851
BmgT120I	GGNCC	1 cut(s)	218
BmiI	GGNNCC	2 cut(s)	277, 749
BmrFI	CCNGG	3 cut(s)	222, 1280, 1371
BmrI	ACTGGG	1 cut(s)	523
BmsI	GCATC	4 cut(s)	31, 118, 413, 1460
BmuI	ACTGGG	1 cut(s)	523
BpiI	GAAGAC	1 cut(s)	1404
BpmI	CTGGAG	1 cut(s)	1301
Bpu10I	CCTNAGC	1 cut(s)	132
BpuEI	CTTGAG	2 cut(s)	527, 1300
BsaBI	GATNNNNATC	1 cut(s)	690
BsaJI	CCNNGG	2 cut(s)	220, 271
BsaXI	ACNNNNNCTCC	4 cut(s)	1242, 1260, 1272, 1290
Bsc4I	CCNNNNNNNGG	2 cut(s)	347, 1282
Bse1I	ACTGG	4 cut(s)	460, 529, 1147, 1438
Bse21I	CCTNAGG	1 cut(s)	1322
Bse3DI	GCAATG	1 cut(s)	791
Bse8I	GATNNNNATC	1 cut(s)	690
BseBI	CCWGG	3 cut(s)	222, 1280, 1371
BseDI	CCNNGG	2 cut(s)	220, 271
BseGI	GGATG	2 cut(s)	355, 723
BseJI	GATNNNNATC	1 cut(s)	690
BseLI	CCNNNNNNNGG	2 cut(s)	347, 1282
BseMI	GCAATG	1 cut(s)	791
BseMII	CTCAG	3 cut(s)	51, 748, 1313
BseNI	ACTGG	4 cut(s)	460, 529, 1147, 1438
BseRI	GAGGAG	2 cut(s)	476, 1339
BseXI	GCAGC	2 cut(s)	167, 247
BsgI	GTGCAG	1 cut(s)	131
Bsh1236I	CGCG	1 cut(s)	715
BshFI	GGCC	1 cut(s)	219
BshNI	GGYRCC	2 cut(s)	275, 747
BsiHKCI	CYCGRG	1 cut(s)	851
BslFI	GGGAC	1 cut(s)	1348
BslI	CCNNNNNNNGG	2 cut(s)	347, 1282
BsmAI	GTCTC	4 cut(s)	451, 959, 1173, 1245
BsmBI	CGTCTC	1 cut(s)	959
BsmFI	GGGAC	1 cut(s)	1348
BsmI	GAATGC	2 cut(s)	1400, 1437
BsnI	GGCC	1 cut(s)	219
BsoBI	CYCGRG	1 cut(s)	851
Bsp143I	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
BspACI	CCGC	3 cut(s)	17, 715, 818
BspANI	GGCC	1 cut(s)	219
BspCNI	CTCAG	3 cut(s)	52, 747, 1314
BspFNI	CGCG	1 cut(s)	715
BspHI	TCATGA	2 cut(s)	571, 594
BspLI	GGNNCC	2 cut(s)	277, 749
BspMAI	CTGCAG	1 cut(s)	157
BspPI	GGATC	2 cut(s)	332, 380
BspT107I	GGYRCC	2 cut(s)	275, 747
BsrBI	CCGCTC	1 cut(s)	818
BsrDI	GCAATG	1 cut(s)	791
BsrI	ACTGG	4 cut(s)	460, 529, 1147, 1438
BssECI	CCNNGG	2 cut(s)	220, 271
BssMI	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
BssT1I	CCWWGG	1 cut(s)	271
Bst2UI	CCWGG	3 cut(s)	222, 1280, 1371
Bst4CI	ACNGT	3 cut(s)	550, 628, 1365
Bst6I	CTCTTC	3 cut(s)	15, 417, 768
BstDEI	CTNAG	5 cut(s)	60, 132, 440, 734, 1322
BstENI	CCTNNNNNAGG	1 cut(s)	1280
BstF5I	GGATG	2 cut(s)	355, 723
BstFNI	CGCG	1 cut(s)	715
BstHHI	GCGC	1 cut(s)	29
BstKTI	GATC	8 cut(s)	340, 375, 571, 589, 688, 706, 733, 1057
BstMAI	GTCTC	4 cut(s)	451, 959, 1173, 1245
BstMBI	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
BstMWI	GCNNNNNNNGC	4 cut(s)	436, 650, 659, 795
BstNI	CCWGG	3 cut(s)	222, 1280, 1371
BstNSI	RCATGY	1 cut(s)	1475
BstSCI	CCNGG	3 cut(s)	220, 1278, 1369
BstSFI	CTRYAG	2 cut(s)	153, 549
BstUI	CGCG	1 cut(s)	715
BstV1I	GCAGC	2 cut(s)	167, 247
BstV2I	GAAGAC	1 cut(s)	1404
BstX2I	RGATCY	1 cut(s)	337
BstYI	RGATCY	1 cut(s)	337
Bsu36I	CCTNAGG	1 cut(s)	1322
BsuRI	GGCC	1 cut(s)	219
BtsCI	GGATG	2 cut(s)	355, 723
BtsIMutI	CAGTG	2 cut(s)	1140, 1361
CaiI	CAGNNNCTG	2 cut(s)	88, 194
CciI	TCATGA	2 cut(s)	571, 594
CfoI	GCGC	1 cut(s)	29
Cfr13I	GGNCC	1 cut(s)	218
Csp6I	GTAC	4 cut(s)	166, 624, 766, 778
CviQI	GTAC	4 cut(s)	166, 624, 766, 778
DdeI	CTNAG	5 cut(s)	60, 132, 440, 734, 1322
DpnI	GATC	8 cut(s)	339, 374, 570, 588, 687, 705, 732, 1056
DpnII	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
Eam1104I	CTCTTC	3 cut(s)	15, 417, 768
EarI	CTCTTC	3 cut(s)	15, 417, 768
EciI	GGCGGA	1 cut(s)	32
Eco130I	CCWWGG	1 cut(s)	271
Eco57I	CTGAAG	4 cut(s)	65, 441, 720, 932
Eco81I	CCTNAGG	1 cut(s)	1322
Eco88I	CYCGRG	1 cut(s)	851
EcoNI	CCTNNNNNAGG	1 cut(s)	1280
EcoO109I	RGGNCCY	1 cut(s)	218
EcoRI	GAATTC	1 cut(s)	173
EcoRII	CCWGG	3 cut(s)	220, 1278, 1369
EcoT14I	CCWWGG	1 cut(s)	271
EcoT22I	ATGCAT	2 cut(s)	99, 791
ErhI	CCWWGG	1 cut(s)	271
Esp3I	CGTCTC	1 cut(s)	959
FalI	AAGNNNNNCTT	2 cut(s)	433, 465
FaqI	GGGAC	1 cut(s)	1348
Fnu4HI	GCNGC	2 cut(s)	156, 261
FokI	GGATG	2 cut(s)	342, 730
Fsp4HI	GCNGC	2 cut(s)	156, 261
FspBI	CTAG	3 cut(s)	50, 335, 1455
FspI	TGCGCA	1 cut(s)	28
GlaI	GCGC	1 cut(s)	28
GluI	GCNGC	2 cut(s)	156, 261
GsuI	CTGGAG	1 cut(s)	1301
HaeIII	GGCC	1 cut(s)	219
HhaI	GCGC	1 cut(s)	29
Hin6I	GCGC	1 cut(s)	27
HinP1I	GCGC	1 cut(s)	27
HincII	GTYRAC	1 cut(s)	1431
HindII	GTYRAC	1 cut(s)	1431
HindIII	AAGCTT	2 cut(s)	482, 1188
HinfI	GANTC	9 cut(s)	235, 283, 575, 635, 899, 1130, 1166, 1253, 1336
HpaI	GTTAAC	1 cut(s)	1431
HphI	GGTGA	4 cut(s)	581, 959, 1064, 1274
Hpy166II	GTNNAC	7 cut(s)	168, 227, 768, 950, 1088, 1119, 1431
Hpy188I	TCNGA	9 cut(s)	61, 391, 607, 700, 708, 737, 865, 912, 1196
Hpy188III	TCNNGA	7 cut(s)	83, 335, 572, 595, 871, 965, 988
Hpy8I	GTNNAC	7 cut(s)	168, 227, 768, 950, 1088, 1119, 1431
HpyAV	CCTTC	7 cut(s)	200, 761, 907, 1153, 1397, 1401, 1486
HpyCH4III	ACNGT	3 cut(s)	550, 628, 1365
HpyCH4IV	ACGT	1 cut(s)	952
HpyF10VI	GCNNNNNNNGC	4 cut(s)	436, 650, 659, 795
HpyF3I	CTNAG	5 cut(s)	60, 132, 440, 734, 1322
HpySE526I	ACGT	1 cut(s)	952
HspAI	GCGC	1 cut(s)	27
KspAI	GTTAAC	1 cut(s)	1431
Kzo9I	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
LmnI	GCTCC	1 cut(s)	463
Lsp1109I	GCAGC	2 cut(s)	167, 247
LweI	GCATC	4 cut(s)	31, 118, 413, 1460
MaeI	CTAG	3 cut(s)	50, 335, 1455
MaeII	ACGT	1 cut(s)	952
MaeIII	GTNAC	4 cut(s)	531, 667, 1220, 1262
MalI	GATC	8 cut(s)	339, 374, 570, 588, 687, 705, 732, 1056
MbiI	CCGCTC	1 cut(s)	818
MboI	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
MflI	RGATCY	1 cut(s)	337
MlyI	GAGTC	7 cut(s)	229, 277, 569, 908, 1175, 1247, 1330
MnlI	CCTC	8 cut(s)	202, 454, 567, 602, 649, 1276, 1286, 1317
Mph1103I	ATGCAT	2 cut(s)	99, 791
MroXI	GAANNNNTTC	2 cut(s)	396, 1411
MseI	TTAA	6 cut(s)	65, 921, 1155, 1380, 1430, 1492
MslI	CAYNNNNRTG	1 cut(s)	1110
MspA1I	CMGCKG	1 cut(s)	410
MspR9I	CCNGG	3 cut(s)	222, 1280, 1371
Mva1269I	GAATGC	2 cut(s)	1400, 1437
MvaI	CCWGG	3 cut(s)	222, 1280, 1371
MvnI	CGCG	1 cut(s)	715
MwoI	GCNNNNNNNGC	4 cut(s)	436, 650, 659, 795
NdeII	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
NlaIV	GGNNCC	2 cut(s)	277, 749
NmuCI	GTSAC	3 cut(s)	531, 1220, 1262
NsbI	TGCGCA	1 cut(s)	28
NsiI	ATGCAT	2 cut(s)	99, 791
NspI	RCATGY	1 cut(s)	1475
PaeR7I	CTCGAG	1 cut(s)	851
PagI	TCATGA	2 cut(s)	571, 594
PctI	GAATGC	2 cut(s)	1400, 1437
PdmI	GAANNNNTTC	2 cut(s)	396, 1411
PfeI	GAWTC	2 cut(s)	635, 1130
PfoI	TCCNGGA	1 cut(s)	1278
PkrI	GCNGC	2 cut(s)	157, 262
PleI	GAGTC	7 cut(s)	229, 277, 569, 907, 1174, 1247, 1330
PpsI	GAGTC	7 cut(s)	229, 277, 569, 907, 1174, 1247, 1330
PsiI	TTATAA	1 cut(s)	1205
Psp6I	CCWGG	3 cut(s)	220, 1278, 1369
PspGI	CCWGG	3 cut(s)	220, 1278, 1369
PspN4I	GGNNCC	2 cut(s)	277, 749
PspPI	GGNCC	1 cut(s)	218
PspXI	VCTCGAGB	1 cut(s)	851
PstI	CTGCAG	1 cut(s)	157
PstNI	CAGNNNCTG	2 cut(s)	88, 194
PsuI	RGATCY	1 cut(s)	337
PvuII	CAGCTG	1 cut(s)	410
RsaI	GTAC	4 cut(s)	167, 625, 767, 779
RsaNI	GTAC	4 cut(s)	166, 624, 766, 778
RseI	CAYNNNNRTG	1 cut(s)	1110
SaqAI	TTAA	6 cut(s)	65, 921, 1155, 1380, 1430, 1492
SatI	GCNGC	2 cut(s)	156, 261
Sau3AI	GATC	8 cut(s)	337, 372, 568, 586, 685, 703, 730, 1054
Sau96I	GGNCC	1 cut(s)	218
SchI	GAGTC	7 cut(s)	229, 277, 569, 908, 1175, 1247, 1330
ScrFI	CCNGG	3 cut(s)	222, 1280, 1371
SfaNI	GCATC	4 cut(s)	31, 118, 413, 1460
SfcI	CTRYAG	2 cut(s)	153, 549
Sfr274I	CTCGAG	1 cut(s)	851
SlaI	CTCGAG	1 cut(s)	851
SmiMI	CAYNNNNRTG	1 cut(s)	1110
SmlI	CTYRAG	3 cut(s)	506, 851, 1315
SmoI	CTYRAG	3 cut(s)	506, 851, 1315
SsiI	CCGC	3 cut(s)	17, 715, 818
SspI	AATATT	1 cut(s)	925
SspMI	CTAG	3 cut(s)	50, 335, 1455
StyD4I	CCNGG	3 cut(s)	220, 1278, 1369
StyI	CCWWGG	1 cut(s)	271
TaaI	ACNGT	3 cut(s)	550, 628, 1365
TaiI	ACGT	1 cut(s)	955
TaqI	TCGA	3 cut(s)	375, 852, 870
TatI	WGTACW	3 cut(s)	165, 623, 765
TfiI	GAWTC	2 cut(s)	635, 1130
Tru1I	TTAA	6 cut(s)	65, 921, 1155, 1380, 1430, 1492
Tru9I	TTAA	6 cut(s)	65, 921, 1155, 1380, 1430, 1492
TscAI	CASTG	2 cut(s)	1147, 1368
TseFI	GTSAC	3 cut(s)	531, 1220, 1262
TseI	GCWGC	2 cut(s)	155, 260
Tsp45I	GTSAC	3 cut(s)	531, 1220, 1262
TspDTI	ATGAA	8 cut(s)	93, 515, 1016, 1026, 1065, 1094, 1107, 1468
TspRI	CASTG	2 cut(s)	1147, 1368
XagI	CCTNNNNNAGG	1 cut(s)	1280
XapI	RAATTY	2 cut(s)	54, 173
XbaI	TCTAGA	1 cut(s)	334
XceI	RCATGY	1 cut(s)	1475
XcmI	CCANNNNNNNNNTGG	1 cut(s)	366
XhoI	CTCGAG	1 cut(s)	851
XmnI	GAANNNNTTC	2 cut(s)	396, 1411
XspI	CTAG	3 cut(s)	50, 335, 1455
Zsp2I	ATGCAT	2 cut(s)	99, 791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.