RLG00000023272

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002353
Physical Location & Seq
Reverse (-)
20985488 .. 20988983
3496 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023272

Sequence Viewer

Length: 729 bp
ATGAGCTCGGCAGATGAGCTGTTCTTGAATGGAAAGATCCAGCCCATGAAGCTCTCCACCCACCTGGAAAAGCCCCAGGCGCCGTTGCAAGTTGTCAACAAGGAAGATGGAGATGATCTATTCGAGTTTGGGCCGCTGAGAGGCAGAGATCTGAGGATGAGGGACAAGTCTCTGCGACGGAGAACGAGATCCATGTCTCCGATGAGAAACATGCCCTTTGGCTGTGAGATTTACGGCGAAAACGAAATCAGTAATAATGACTGTTGCTTTGCGAAAGATCTGAAGGAGGGTGATTTGGGGAAGAAGAAGAAGAAGCCGGAGAATGGTGAGGAGGAGGAGGAGGAAGAAGTTGGGAGCTTGGAGACTACTCCTTCGGTTTCGGCTTCGTCTTCAAGATCATCCTCAACTGGAAGAAACTCGAAAAGATGGGTCTTTCTGAAAGATTTTCTCCACAAGAGTAAAAGTGAAGGGAGGAGTAACAACAAATTCTGGGACACAATCTCATTCTCGAGTAGTGGTAAGGAGAAGAAAATAGAGGCAAGTGTTGGGGGAGAAAATAGGCAGAAAATTGGCTCAGTGGAGTCGGACACGGAGGTGATTGAACAGAGCAATGATATGAGTCGGAATCCTATACAGGAGGTTGTTCCTGAGCCATCTCCTGGAACACATAGGCATGTGGCGAAGTCCCCAATGGTGCAGGAGATAGTCCACCCCAATAGATTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

243

Amino Acids

27.31

Weight (kDa)

6.56

Isoelectric Point (pI)

70.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 4 - 189 2.9e-27 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 659
AciI CCGC 1 cut(s) 134
AclWI GGATC 2 cut(s) 31, 183
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 80
AfiI CCNNNNNNNGG 3 cut(s) 140, 323, 659
AgsI TTSAA 4 cut(s) 28, 393, 602, 725
AjnI CCWGG 3 cut(s) 63, 75, 658
AleI CACNNNNGTG 1 cut(s) 593
AluBI AGCT 4 cut(s) 6, 19, 52, 357
AluI AGCT 4 cut(s) 6, 19, 52, 357
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 3 cut(s) 174, 201, 356
AlwI GGATC 2 cut(s) 31, 183
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 1 cut(s) 131
ApoI RAATTY 1 cut(s) 485
ArsI GACNNNNNNTTYG 4 cut(s) 251, 283, 355, 387
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 3 cut(s) 302, 338, 607
AvaI CYCGRG 1 cut(s) 508
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 8
BccI CCATC 3 cut(s) 101, 420, 661
BceAI ACGGC 2 cut(s) 67, 250
BciT130I CCWGG 3 cut(s) 65, 77, 660
BcoDI GTCTC 3 cut(s) 174, 201, 356
BfoI RGCGCY 1 cut(s) 83
BglII AGATCT 2 cut(s) 148, 277
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
Bme1390I CCNGG 3 cut(s) 65, 77, 660
BmeT110I CYCGRG 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 3 cut(s) 65, 77, 660
BpiI GAAGAC 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 648
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 75
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 323, 659
Bse1I ACTGG 1 cut(s) 412
Bse3DI GCAATG 1 cut(s) 616
BseBI CCWGG 3 cut(s) 65, 77, 660
BseDI CCNNGG 1 cut(s) 75
BseGI GGATG 2 cut(s) 162, 398
BseLI CCNNNNNNNGG 3 cut(s) 140, 323, 659
BseMI GCAATG 1 cut(s) 616
BseMII CTCAG 4 cut(s) 128, 143, 588, 639
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 5 cut(s) 344, 347, 350, 353, 487
BsgI GTGCAG 1 cut(s) 716
BshFI GGCC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 1 cut(s) 317
BslFI GGGAC 3 cut(s) 176, 506, 670
BslI CCNNNNNNNGG 3 cut(s) 140, 323, 659
BsmAI GTCTC 3 cut(s) 174, 201, 356
BsmFI GGGAC 3 cut(s) 176, 506, 670
BsnI GGCC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 6 cut(s) 36, 115, 148, 188, 277, 395
BspACI CCGC 1 cut(s) 134
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 4 cut(s) 129, 144, 587, 640
BspLI GGNNCC 1 cut(s) 81
BspPI GGATC 2 cut(s) 31, 183
BspT107I GGYRCC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 616
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 6 cut(s) 36, 115, 148, 188, 277, 395
BssNI GRCGYC 1 cut(s) 80
Bst2UI CCWGG 3 cut(s) 65, 77, 660
Bst4CI ACNGT 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 80
BstDEI CTNAG 4 cut(s) 137, 152, 574, 648
BstF5I GGATG 2 cut(s) 162, 398
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 6 cut(s) 39, 118, 151, 191, 280, 398
BstMAI GTCTC 3 cut(s) 174, 201, 356
BstMBI GATC 6 cut(s) 36, 115, 148, 188, 277, 395
BstMWI GCNNNNNNNGC 2 cut(s) 49, 79
BstNI CCWGG 3 cut(s) 65, 77, 660
BstNSI RCATGY 2 cut(s) 214, 677
BstSCI CCNGG 3 cut(s) 63, 75, 658
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 4 cut(s) 36, 148, 188, 277
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 4 cut(s) 36, 148, 188, 277
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 2 cut(s) 162, 398
BtsIMutI CAGTG 1 cut(s) 582
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 131
CviAII CATG 4 cut(s) 46, 193, 211, 674
DdeI CTNAG 4 cut(s) 137, 152, 574, 648
DinI GGCGCC 1 cut(s) 81
DpnI GATC 6 cut(s) 38, 117, 150, 190, 279, 397
DpnII GATC 6 cut(s) 36, 115, 148, 188, 277, 395
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 508
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 3 cut(s) 63, 75, 658
EcoT38I GRGCYC 1 cut(s) 8
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
FaeI CATG 4 cut(s) 49, 196, 214, 677
FaiI YATR 7 cut(s) 47, 194, 212, 617, 632, 669, 675
FaqI GGGAC 3 cut(s) 176, 506, 670
FatI CATG 4 cut(s) 45, 192, 210, 673
Fnu4HI GCNGC 1 cut(s) 134
FokI GGATG 2 cut(s) 169, 385
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 1 cut(s) 134
GlaI GCGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 317
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 4 cut(s) 49, 196, 214, 677
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 3 cut(s) 581, 619, 625
HpaII CCGG 1 cut(s) 317
HphI GGTGA 3 cut(s) 302, 338, 607
Hpy166II GTNNAC 2 cut(s) 97, 709
Hpy188I TCNGA 6 cut(s) 153, 201, 282, 438, 586, 624
Hpy188III TCNNGA 4 cut(s) 25, 393, 508, 647
Hpy8I GTNNAC 2 cut(s) 97, 709
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 3 cut(s) 277, 381, 461
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 88, 697
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 79
HpyF3I CTNAG 4 cut(s) 137, 152, 574, 648
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 4 cut(s) 49, 196, 214, 677
HspAI GCGC 1 cut(s) 80
KasI GGCGCC 1 cut(s) 79
Kzo9I GATC 6 cut(s) 36, 115, 148, 188, 277, 395
LmnI GCTCC 1 cut(s) 354
MaeIII GTNAC 1 cut(s) 476
MalI GATC 6 cut(s) 38, 117, 150, 190, 279, 397
MboI GATC 6 cut(s) 36, 115, 148, 188, 277, 395
MboII GAAGA 9 cut(s) 116, 313, 316, 319, 322, 356, 381, 423, 538
MflI RGATCY 4 cut(s) 36, 148, 188, 277
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 485, 567
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 2 cut(s) 590, 628
MmeI TCCRAC 2 cut(s) 564, 602
MslI CAYNNNNRTG 2 cut(s) 593, 672
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 3 cut(s) 65, 77, 660
MvaI CCWGG 3 cut(s) 65, 77, 660
MwoI GCNNNNNNNGC 2 cut(s) 49, 79
NarI GGCGCC 1 cut(s) 80
NdeII GATC 6 cut(s) 36, 115, 148, 188, 277, 395
NlaIII CATG 4 cut(s) 49, 196, 214, 677
NlaIV GGNNCC 1 cut(s) 81
NspI RCATGY 2 cut(s) 214, 677
OliI CACNNNNGTG 1 cut(s) 593
PaeR7I CTCGAG 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 1 cut(s) 625
PflMI CCANNNNNTGG 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 1 cut(s) 135
PleI GAGTC 2 cut(s) 589, 627
PluTI GGCGCC 1 cut(s) 83
PpsI GAGTC 2 cut(s) 589, 627
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 63, 75, 658
PspGI CCWGG 3 cut(s) 63, 75, 658
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 131
PsuI RGATCY 4 cut(s) 36, 148, 188, 277
RseI CAYNNNNRTG 2 cut(s) 593, 672
SacI GAGCTC 1 cut(s) 8
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 6 cut(s) 36, 115, 148, 188, 277, 395
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 2 cut(s) 590, 628
ScrFI CCNGG 3 cut(s) 65, 77, 660
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 8, 21, 54, 66, 359, 597, 642
SfoI GGCGCC 1 cut(s) 81
Sfr274I CTCGAG 1 cut(s) 508
SlaI CTCGAG 1 cut(s) 508
SmiMI CAYNNNNRTG 2 cut(s) 593, 672
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 2 cut(s) 485, 567
SsiI CCGC 1 cut(s) 134
SspDI GGCGCC 1 cut(s) 79
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 63, 75, 658
TaaI ACNGT 1 cut(s) 263
TaqI TCGA 3 cut(s) 123, 419, 509
TasI AATT 2 cut(s) 485, 567
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 1 cut(s) 625
TscAI CASTG 1 cut(s) 582
TspDTI ATGAA 1 cut(s) 62
TspGWI ACGGA 2 cut(s) 193, 605
TspRI CASTG 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 659
XapI RAATTY 1 cut(s) 485
XceI RCATGY 2 cut(s) 214, 677
XhoI CTCGAG 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.