pycom12g19510

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002353
Physical Location & Seq
Reverse (-)
21326250 .. 21327491
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19510.1

Sequence Viewer

Length: 1242 bp
ATGGAGCTCAAAACTTCACCCGGCAATGGCACCCAACCCCCAAACCCTGAACCGAAACCCGACCCTCCAAACCCTAGCAATGGCGAAAGCCAGCTTCAAGACCAGCTTTTCTTTGAAGACTACGACAGCACCTGCTCCACGCCTTACGTCAGCGCGCCGTCGAGCCCCGGACGGAGTGGGACGGTCGGTGGTTTTTTCTACAGTGCTCCGGCGAGCCCAATGCACTTCGGGTTGACCACAAAGTCAAACTCTTGTTCGCTTGAGAAGAATGGCGGCAACGGCTCTGTTTCTATGGGTTTTGAGTTCGAGTTCTCTGCGCGGTTCGGGTCGAGCGGGTCGGGCTCGACCGGGCCGATGAGCTCTGCCGACGAGTTGTTCTTGAACGGGAAGATCCGACCCATGAAGCTTTCGACCCACCTGGAAAAGCCCCAGACTCTGGCTCCGTTGTTGGATCTCGAGGCGGAAAATGGGGAAGACGAGGCGGATCGGGGCGGTTTGGAGCCCGTCAGAGGCAGAGATCTGAGGTTCCGCGACAAGTCGCTGCGGCGGAGGACGAGATCTATGTCCCCTCTGAGAAATGAGCCCTTCGAGTGGGAACAGAGCGACGAGATTATTGAAGCTGATGAGAAAGACTGCAACTTAGCTGAAGATCCGAAAGTGGTGGCGAAAAATGGGGAAGAAGACGAAGAGGGAGCGGAGGAGAGCAGCACCGCCACTACTACTCCTTCGGTTTCGGCTTCGTCGTCTCGGTCGTCGTCGGGTGGGAGGAACTCGAAGAAGTGGGTTTTTTTGAAGGAGTTTCTTCACCGGAGTAAAAGCGAAGGGCGGAGCAGCAACCACAAGTTCTGGTCGTCGATTTCGTTCTCCGCCGCGAAGGAGAAAAAACCCATGAAACAGGGCGGTCAGAATACCCAGGGCTCTGCTCTAAAAGACCCCAAATGTAGCAACCCTTTGAACTTGGGCTCAGAGGGGCAAAAACTGAAGGGAAGTGGGGGTGGTGGTTCGGGGAAGAAGATGGCGGCTGGAAAACCCACCAACGGAGTTGGTAAGCGGCGGATGGCCCCGTCGCTTCACGAGCTGCATTACACTGCAAACAGGGCTCAGGCAGAGGAAATGAGGAAGAAGACTTACTTACCTTACAGGCAAGGGTTGCTTGGATGCTTAGGGTTTAGCTCAAAGGGTTATGGAGCTGCTATGAACTCTGGGTTTGCTAGGGCTTTGAATCCTGTTTCTTCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

414

Amino Acids

44.42

Weight (kDa)

8.78

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 122 - 379 2.3e-43 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 140
AasI GACNNNNNNGTC 1 cut(s) 241
Acc36I ACCTGC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 436
AccBSI CCGCTC 2 cut(s) 333, 695
AccII CGCG 4 cut(s) 155, 319, 531, 872
AclWI GGATC 4 cut(s) 385, 459, 492, 644
AcuI CTGAAG 2 cut(s) 666, 1001
AfiI CCNNNNNNNGG 6 cut(s) 26, 80, 436, 509, 591, 1037
AgsI TTSAA 8 cut(s) 98, 116, 382, 617, 793, 955, 1222, 1236
AjnI CCWGG 2 cut(s) 417, 912
Alw21I GWGCWC 3 cut(s) 9, 208, 362
Alw26I GTCTC 1 cut(s) 750
AlwI GGATC 4 cut(s) 385, 459, 492, 644
AlwNI CAGNNNCTG 2 cut(s) 132, 436
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 2 cut(s) 350, 1059
ApeKI GCWGC 5 cut(s) 541, 705, 831, 1078, 1190
AspLEI GCGC 3 cut(s) 155, 157, 319
AspS9I GGNCC 2 cut(s) 350, 1060
AsuC2I CCSGG 3 cut(s) 21, 168, 349
AsuHPI GGTGA 2 cut(s) 9, 797
AvaI CYCGRG 1 cut(s) 455
BanI GGYRCC 1 cut(s) 29
BarI GAAGNNNNNNTAC 2 cut(s) 1112, 1144
BauI CACGAG 1 cut(s) 1073
BbsI GAAGAC 4 cut(s) 123, 480, 687, 1130
Bbv12I GWGCWC 3 cut(s) 9, 208, 362
BbvI GCAGC 5 cut(s) 528, 717, 843, 1065, 1177
BccI CCATC 2 cut(s) 1009, 1051
BceAI ACGGC 2 cut(s) 142, 295
BcgI CGANNNNNNTGC 4 cut(s) 202, 236, 296, 330
BciT130I CCWGG 2 cut(s) 419, 914
BcnI CCSGG 3 cut(s) 21, 168, 349
BcoDI GTCTC 1 cut(s) 750
BfaI CTAG 2 cut(s) 75, 1212
BfmI CTRYAG 1 cut(s) 199
BfuAI ACCTGC 1 cut(s) 140
BglII AGATCT 2 cut(s) 517, 557
Bme1390I CCNGG 5 cut(s) 21, 168, 349, 419, 914
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 2 cut(s) 350, 1060
BmiI GGNNCC 5 cut(s) 31, 441, 501, 527, 1062
BmrFI CCNGG 5 cut(s) 21, 168, 349, 419, 914
BmsI GCATC 1 cut(s) 1148
BpiI GAAGAC 4 cut(s) 123, 480, 687, 1130
Bpu10I CCTNAGC 2 cut(s) 1101, 1162
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 3 cut(s) 21, 168, 349
BsaJI CCNNGG 3 cut(s) 166, 912, 913
BsaWI WCCGGW 1 cut(s) 807
BsaXI ACNNNNNCTCC 4 cut(s) 424, 454, 706, 736
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 80, 436, 509, 591, 1037
Bse3DI GCAATG 2 cut(s) 31, 85
BseBI CCWGG 2 cut(s) 419, 914
BseDI CCNNGG 3 cut(s) 166, 912, 913
BseGI GGATG 2 cut(s) 1062, 1163
BseLI CCNNNNNNNGG 6 cut(s) 26, 80, 436, 509, 591, 1037
BseMI GCAATG 2 cut(s) 31, 85
BseMII CTCAG 4 cut(s) 512, 563, 978, 1115
BsePI GCGCGC 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 5 cut(s) 528, 717, 843, 1065, 1177
Bsh1236I CGCG 4 cut(s) 155, 319, 531, 872
Bsh1285I CGRYCG 3 cut(s) 186, 348, 752
BshFI GGCC 2 cut(s) 352, 1061
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 3 cut(s) 186, 348, 752
BsiHKAI GWGCWC 3 cut(s) 9, 208, 362
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 5 cut(s) 21, 168, 209, 348, 808
BslFI GGGAC 2 cut(s) 193, 550
BslI CCNNNNNNNGG 6 cut(s) 26, 80, 436, 509, 591, 1037
BsmAI GTCTC 1 cut(s) 750
BsmBI CGTCTC 1 cut(s) 750
BsmFI GGGAC 2 cut(s) 193, 550
BsnI GGCC 2 cut(s) 352, 1061
BsoBI CYCGRG 1 cut(s) 455
Bsp143I GATC 6 cut(s) 390, 451, 484, 517, 557, 649
BspANI GGCC 2 cut(s) 352, 1061
BspCNI CTCAG 4 cut(s) 513, 564, 977, 1114
BspFNI CGCG 4 cut(s) 155, 319, 531, 872
BspLI GGNNCC 5 cut(s) 31, 441, 501, 527, 1062
BspMI ACCTGC 1 cut(s) 140
BspPI GGATC 4 cut(s) 385, 459, 492, 644
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 2 cut(s) 333, 695
BsrDI GCAATG 2 cut(s) 31, 85
BssECI CCNNGG 3 cut(s) 166, 912, 913
BssHII GCGCGC 1 cut(s) 153
BssMI GATC 6 cut(s) 390, 451, 484, 517, 557, 649
BssSI CACGAG 1 cut(s) 1073
Bst2BI CACGAG 1 cut(s) 1073
Bst2UI CCWGG 2 cut(s) 419, 914
Bst4CI ACNGT 2 cut(s) 184, 203
Bst6I CTCTTC 1 cut(s) 681
BstAPI GCANNNNNTGC 1 cut(s) 1150
BstC8I GCNNGC 3 cut(s) 92, 155, 214
BstDEI CTNAG 6 cut(s) 521, 572, 640, 964, 1101, 1162
BstF5I GGATG 2 cut(s) 1062, 1163
BstFNI CGCG 4 cut(s) 155, 319, 531, 872
BstHHI GCGC 3 cut(s) 155, 157, 319
BstKTI GATC 6 cut(s) 393, 454, 487, 520, 560, 652
BstMAI GTCTC 1 cut(s) 750
BstMBI GATC 6 cut(s) 390, 451, 484, 517, 557, 649
BstMCI CGRYCG 3 cut(s) 186, 348, 752
BstMWI GCNNNNNNNGC 5 cut(s) 279, 339, 1075, 1097, 1150
BstNI CCWGG 2 cut(s) 419, 914
BstSCI CCNGG 5 cut(s) 19, 166, 347, 417, 912
BstSFI CTRYAG 1 cut(s) 199
BstUI CGCG 4 cut(s) 155, 319, 531, 872
BstV1I GCAGC 5 cut(s) 528, 717, 843, 1065, 1177
BstV2I GAAGAC 4 cut(s) 123, 480, 687, 1130
BstX2I RGATCY 5 cut(s) 390, 451, 517, 557, 649
BstYI RGATCY 5 cut(s) 390, 451, 517, 557, 649
BsuRI GGCC 2 cut(s) 352, 1061
BtsCI GGATG 2 cut(s) 1062, 1163
BtsI GCAGTG 1 cut(s) 1086
BtsIMutI CAGTG 2 cut(s) 208, 1086
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 3 cut(s) 92, 155, 214
CaiI CAGNNNCTG 2 cut(s) 132, 436
CfoI GCGC 3 cut(s) 155, 157, 319
Cfr13I GGNCC 2 cut(s) 350, 1060
CviAII CATG 2 cut(s) 400, 889
DdeI CTNAG 6 cut(s) 521, 572, 640, 964, 1101, 1162
DpnI GATC 6 cut(s) 392, 453, 486, 519, 559, 651
DpnII GATC 6 cut(s) 390, 451, 484, 517, 557, 649
DrdI GACNNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
EciI GGCGGA 6 cut(s) 476, 497, 562, 841, 856, 1069
Ecl136II GAGCTC 2 cut(s) 7, 360
Eco53kI GAGCTC 2 cut(s) 7, 360
Eco57I CTGAAG 2 cut(s) 666, 1001
Eco88I CYCGRG 1 cut(s) 455
EcoICRI GAGCTC 2 cut(s) 7, 360
EcoRII CCWGG 2 cut(s) 417, 912
Esp3I CGTCTC 1 cut(s) 750
FaeI CATG 2 cut(s) 403, 892
FaiI YATR 6 cut(s) 293, 401, 563, 890, 1185, 1196
FalI AAGNNNNNCTT 6 cut(s) 90, 122, 1115, 1147, 1137, 1169
FaqI GGGAC 2 cut(s) 193, 550
FatI CATG 2 cut(s) 399, 888
FauI CCCGC 1 cut(s) 326
FokI GGATG 2 cut(s) 1069, 1170
FspBI CTAG 2 cut(s) 75, 1212
GlaI GCGC 3 cut(s) 154, 156, 318
HaeIII GGCC 2 cut(s) 352, 1061
HapII CCGG 5 cut(s) 21, 168, 209, 348, 808
HhaI GCGC 3 cut(s) 155, 157, 319
Hin1II CATG 2 cut(s) 403, 892
Hin6I GCGC 3 cut(s) 153, 155, 317
HinP1I GCGC 3 cut(s) 153, 155, 317
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 2 cut(s) 433, 1222
HpaII CCGG 5 cut(s) 21, 168, 209, 348, 808
HphI GGTGA 2 cut(s) 9, 797
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 7 cut(s) 395, 509, 522, 573, 654, 906, 967
Hpy188III TCNNGA 4 cut(s) 98, 379, 455, 1073
Hpy8I GTNNAC 1 cut(s) 234
Hpy99I CGWCG 8 cut(s) 163, 371, 608, 745, 757, 760, 856, 1069
HpyAV CCTTC 6 cut(s) 595, 735, 787, 815, 868, 976
HpyCH4III ACNGT 2 cut(s) 184, 203
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 4 cut(s) 223, 636, 1081, 1091
HpyF10VI GCNNNNNNNGC 5 cut(s) 279, 339, 1075, 1097, 1150
HpyF3I CTNAG 6 cut(s) 521, 572, 640, 964, 1101, 1162
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 2 cut(s) 403, 892
HspAI GCGC 3 cut(s) 153, 155, 317
Kzo9I GATC 6 cut(s) 390, 451, 484, 517, 557, 649
LmnI GCTCC 8 cut(s) 4, 140, 211, 445, 499, 692, 828, 1187
Lsp1109I GCAGC 5 cut(s) 528, 717, 843, 1065, 1177
LweI GCATC 1 cut(s) 1148
MaeI CTAG 2 cut(s) 75, 1212
MaeII ACGT 1 cut(s) 147
MalI GATC 6 cut(s) 392, 453, 486, 519, 559, 651
MbiI CCGCTC 2 cut(s) 333, 695
MboI GATC 6 cut(s) 390, 451, 484, 517, 557, 649
MflI RGATCY 5 cut(s) 390, 451, 517, 557, 649
MlyI GAGTC 1 cut(s) 427
MmeI TCCRAC 2 cut(s) 418, 429
MspI CCGG 5 cut(s) 21, 168, 209, 348, 808
MspR9I CCNGG 5 cut(s) 21, 168, 349, 419, 914
MvaI CCWGG 2 cut(s) 419, 914
MvnI CGCG 4 cut(s) 155, 319, 531, 872
MwoI GCNNNNNNNGC 5 cut(s) 279, 339, 1075, 1097, 1150
NciI CCSGG 3 cut(s) 21, 168, 349
NdeII GATC 6 cut(s) 390, 451, 484, 517, 557, 649
NlaIII CATG 2 cut(s) 403, 892
NlaIV GGNNCC 5 cut(s) 31, 441, 501, 527, 1062
PaeR7I CTCGAG 1 cut(s) 455
PaqCI CACCTGC 1 cut(s) 140
PasI CCCWGGG 1 cut(s) 913
PauI GCGCGC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 4 cut(s) 350, 740, 749, 857
PfeI GAWTC 1 cut(s) 1222
PflMI CCANNNNNTGG 1 cut(s) 436
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp124BI GAGCTC 2 cut(s) 9, 362
Psp6I CCWGG 2 cut(s) 417, 912
PspGI CCWGG 2 cut(s) 417, 912
PspN4I GGNNCC 5 cut(s) 31, 441, 501, 527, 1062
PspPI GGNCC 2 cut(s) 350, 1060
PstNI CAGNNNCTG 2 cut(s) 132, 436
PsuI RGATCY 5 cut(s) 390, 451, 517, 557, 649
PteI GCGCGC 1 cut(s) 153
SacI GAGCTC 2 cut(s) 9, 362
Sau3AI GATC 6 cut(s) 390, 451, 484, 517, 557, 649
Sau96I GGNCC 2 cut(s) 350, 1060
SchI GAGTC 1 cut(s) 427
ScrFI CCNGG 5 cut(s) 21, 168, 349, 419, 914
SfaNI GCATC 1 cut(s) 1148
SfcI CTRYAG 1 cut(s) 199
Sfr274I CTCGAG 1 cut(s) 455
SlaI CTCGAG 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 260, 455
SmoI CTYRAG 2 cut(s) 260, 455
SspMI CTAG 2 cut(s) 75, 1212
SstI GAGCTC 2 cut(s) 9, 362
StyD4I CCNGG 5 cut(s) 19, 166, 347, 417, 912
TaaI ACNGT 2 cut(s) 184, 203
TaiI ACGT 1 cut(s) 150
TaqI TCGA 9 cut(s) 161, 306, 329, 344, 410, 456, 588, 773, 854
TaqII GACCGA 1 cut(s) 738
TauI GCSGC 5 cut(s) 276, 547, 872, 1022, 1054
TfiI GAWTC 1 cut(s) 1222
TscAI CASTG 2 cut(s) 208, 1093
TseI GCWGC 5 cut(s) 541, 705, 831, 1078, 1190
TspDTI ATGAA 3 cut(s) 416, 905, 1211
TspGWI ACGGA 3 cut(s) 187, 432, 1053
TspRI CASTG 2 cut(s) 208, 1093
Van91I CCANNNNNTGG 1 cut(s) 436
XhoI CTCGAG 1 cut(s) 455
XspI CTAG 2 cut(s) 75, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.