FvH4_6g07220

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002353
Physical Location & Seq
Forward (+)
4352882 .. 4355434
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07220.t1

Sequence Viewer

Length: 1194 bp
ATGGAGCTCAAACCAACCTCCAACAATGGCCAAACCTCAACCCCTGAGAATGAGCCTAACCCTAGCAATGGCGAAACCCACGACCCCCATTTCTTCTACGAAGACATTGACAGCAACTGCTCCACTCCTTACGTCAGCGCTCCCTCCAGCCCCGGCCGCGGCCCCATCTCCGGTTTCTTCTACAGTGCACCGGCGAGCCCAATGCACTTTGTGCTCTCTTCCGCTTCGATGAACAATCACAACTCTTCTTGTTTTGATACTTCCGCGTCGGAGTTCGAGTTCTCAGCTCGATTCGGGTCCGGTGGATCTAGTGCCACCGGGCCGATGAGCTCCGCGGATGAGTTGTTCTTGGATGGAAAGATCCGGCCAATGAAGCTGTCGACCCACCTGGAGCAGCCGCAGGCGCCGCTGCTCGATCTGCAAGGTGTCAATGAGGAAAATGGTGTCGAGGTGTTCGAGTTCGGGCCGATGAGGGGGAGGGATCTGAGGTTTAGGGACAAGTCGCTGCGGAGGAGGACTAGATCCATGTCGCCGATGAGAAACATGCCTTTCGGGTGCGAGATTTACGGCGATAATGAAATCAGTACTGATCATTCTTGTTTTGATAAAGATCCAGAGCATGGCGAGTTGGGGAAGAAGGCGGAGAATGGCGAGGAGGAGGAGGAAGTTGGGAGCTTGGAGACTACTCCTTCGGTTTCGGCCTCGTCTTCGAGGTCGTCCTCAGCTGGAAGAAACTCGAAACGATGGGTGTTTCTGAAGGATTTTCTTCACAGGAGTAAAAGTGAAGGAAGAAGCAACAACAAATTCTGGGCCACAATCTCATTCTCTGGAAGTGGCAAAGAGAAGAAGACAATGAAGAGTGGGAGTCAAAATAAGGAGGGTTTGAGTTTGGGCCCGGAGCCGGCGACTCAGAAGGCCAAGGCGGCAATTGGGTCTGCTTCAGGGAAGAAGCCGGTGGCCGGAAAGCCAGCCAATGGAGTAGGGAAGAGGAGGATGGTGCCGTCGCTTCATGAGCTGCATTACACTGCTAATAGAGCTCAGGCGGAGGAAATGAGGAAGAAGACTTACTTGCCCTACAGGCAAGGATTGCTTGGATGCTTGGGTTTTAGCTCGAAGGGGTATGGAGCTGCCATGAGTACTGGGTTTGCCAGAGCACTAAACCCAGTCTCTTCAAGGACACATCGCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

398

Amino Acids

43.21

Weight (kDa)

8.93

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 112 - 358 3.9e-43 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 403, 997
AccB7I CCANNNNNTGG 2 cut(s) 620, 974
AccI GTMKAC 1 cut(s) 380
AccII CGCG 3 cut(s) 159, 266, 335
AclWI GGATC 5 cut(s) 313, 355, 489, 516, 605
AcoI YGGCCR 4 cut(s) 28, 154, 365, 957
AcsI RAATTY 1 cut(s) 803
AcuI CTGAAG 2 cut(s) 776, 924
AcyI GRCGYC 1 cut(s) 404
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 2 cut(s) 586, 1138
AfeI AGCGCT 1 cut(s) 139
AfiI CCNNNNNNNGG 8 cut(s) 68, 158, 170, 473, 620, 901, 959, 974
AgsI TTSAA 1 cut(s) 1173
AjnI CCWGG 1 cut(s) 387
AloI GAACNNNNNNTCC 4 cut(s) 263, 295, 329, 361
Alw21I GWGCWC 6 cut(s) 9, 190, 216, 332, 1039, 1156
Alw26I GTCTC 2 cut(s) 674, 1171
Alw44I GTGCAC 1 cut(s) 186
AlwI GGATC 5 cut(s) 313, 355, 489, 516, 605
AlwNI CAGNNNCTG 1 cut(s) 117
Aor51HI AGCGCT 1 cut(s) 139
ApaI GGGCCC 1 cut(s) 896
ApaLI GTGCAC 1 cut(s) 186
ApeKI GCWGC 5 cut(s) 394, 409, 505, 1015, 1127
ApoI RAATTY 1 cut(s) 803
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
AspLEI GCGC 2 cut(s) 140, 406
AspS9I GGNCC 7 cut(s) 161, 297, 320, 464, 810, 892, 893
AsuC2I CCSGG 3 cut(s) 153, 319, 896
AvaII GGWCC 1 cut(s) 297
BaeGI GKGCMC 2 cut(s) 190, 896
BalI TGGCCA 1 cut(s) 30
BanI GGYRCC 2 cut(s) 403, 997
BanII GRGCYC 5 cut(s) 9, 200, 332, 896, 1039
BarI GAAGNNNNNNTAC 2 cut(s) 1049, 1081
BbsI GAAGAC 4 cut(s) 108, 699, 854, 1067
Bbv12I GWGCWC 6 cut(s) 9, 190, 216, 332, 1039, 1156
BbvCI CCTCAGC 1 cut(s) 721
BbvI GCAGC 5 cut(s) 396, 406, 492, 1002, 1114
BccI CCATC 4 cut(s) 173, 347, 738, 988
BceAI ACGGC 2 cut(s) 583, 985
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 3 cut(s) 153, 319, 896
BcoDI GTCTC 2 cut(s) 674, 1171
BfaI CTAG 3 cut(s) 63, 309, 519
BfmI CTRYAG 2 cut(s) 181, 1075
BfoI RGCGCY 2 cut(s) 141, 407
BglI GCCNNNNNGGC 2 cut(s) 923, 1078
BmcAI AGTACT 2 cut(s) 586, 1138
Bme1390I CCNGG 4 cut(s) 153, 319, 389, 896
Bme18I GGWCC 1 cut(s) 297
BmgT120I GGNCC 7 cut(s) 161, 297, 320, 464, 810, 892, 893
BmiI GGNNCC 6 cut(s) 163, 298, 405, 894, 900, 999
BmrFI CCNGG 4 cut(s) 153, 319, 389, 896
BmrI ACTGGG 2 cut(s) 1149, 1157
BmsI GCATC 1 cut(s) 1085
BmuI ACTGGG 2 cut(s) 1149, 1157
BpiI GAAGAC 4 cut(s) 108, 699, 854, 1067
BpmI CTGGAG 2 cut(s) 130, 410
Bpu10I CCTNAGC 2 cut(s) 721, 1038
BpuMI CCSGG 3 cut(s) 153, 319, 896
BsaBI GATNNNNATC 1 cut(s) 609
BsaHI GRCGYC 1 cut(s) 404
BsaJI CCNNGG 4 cut(s) 151, 157, 333, 918
BsaWI WCCGGW 2 cut(s) 170, 299
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 766, 796
Bsc4I CCNNNNNNNGG 8 cut(s) 68, 158, 170, 473, 620, 901, 959, 974
Bse118I RCCGGY 3 cut(s) 190, 901, 952
Bse1I ACTGG 2 cut(s) 1144, 1163
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 4 cut(s) 151, 157, 333, 918
BseGI GGATG 4 cut(s) 343, 358, 999, 1100
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 8 cut(s) 68, 158, 170, 473, 620, 901, 959, 974
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 6 cut(s) 36, 297, 476, 735, 923, 1052
BseNI ACTGG 2 cut(s) 1144, 1163
BseRI GAGGAG 5 cut(s) 526, 668, 671, 674, 1003
BseSI GKGCMC 2 cut(s) 190, 896
BseX3I CGGCCG 1 cut(s) 154
BseXI GCAGC 5 cut(s) 396, 406, 492, 1002, 1114
Bsh1236I CGCG 3 cut(s) 159, 266, 335
Bsh1285I CGRYCG 1 cut(s) 157
BshNI GGYRCC 2 cut(s) 403, 997
BsiEI CGRYCG 1 cut(s) 157
BsiHKAI GWGCWC 6 cut(s) 9, 190, 216, 332, 1039, 1156
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 8 cut(s) 68, 158, 170, 473, 620, 901, 959, 974
BsmAI GTCTC 2 cut(s) 674, 1171
BsmFI GGGAC 1 cut(s) 509
Bsp120I GGGCCC 1 cut(s) 892
Bsp1286I GDGCHC 8 cut(s) 9, 190, 200, 216, 332, 896, 1039, 1156
Bsp143I GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
BspCNI CTCAG 6 cut(s) 37, 296, 477, 734, 922, 1051
BspFNI CGCG 3 cut(s) 159, 266, 335
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 6 cut(s) 163, 298, 405, 894, 900, 999
BspPI GGATC 5 cut(s) 313, 355, 489, 516, 605
BspT107I GGYRCC 2 cut(s) 403, 997
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 3 cut(s) 190, 901, 952
BsrI ACTGG 2 cut(s) 1144, 1163
BssAI RCCGGY 3 cut(s) 190, 901, 952
BssECI CCNNGG 4 cut(s) 151, 157, 333, 918
BssMI GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
BssNI GRCGYC 1 cut(s) 404
BssT1I CCWWGG 1 cut(s) 918
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 185
Bst6I CTCTTC 5 cut(s) 223, 250, 851, 980, 1174
BstACI GRCGYC 1 cut(s) 404
BstAPI GCANNNNNTGC 2 cut(s) 211, 1087
BstC8I GCNNGC 4 cut(s) 196, 402, 903, 969
BstDEI CTNAG 6 cut(s) 45, 283, 485, 721, 909, 1038
BstDSI CCRYGG 2 cut(s) 157, 333
BstF5I GGATG 4 cut(s) 343, 358, 999, 1100
BstFNI CGCG 3 cut(s) 159, 266, 335
BstH2I RGCGCY 2 cut(s) 141, 407
BstHHI GCGC 2 cut(s) 140, 406
BstKTI GATC 7 cut(s) 308, 363, 418, 484, 524, 592, 613
BstMAI GTCTC 2 cut(s) 674, 1171
BstMBI GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
BstMCI CGRYCG 1 cut(s) 157
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 4 cut(s) 151, 317, 387, 894
BstSFI CTRYAG 2 cut(s) 181, 1075
BstSLI GKGCMC 2 cut(s) 190, 896
BstUI CGCG 3 cut(s) 159, 266, 335
BstV1I GCAGC 5 cut(s) 396, 406, 492, 1002, 1114
BstV2I GAAGAC 4 cut(s) 108, 699, 854, 1067
BstX2I RGATCY 5 cut(s) 305, 360, 481, 521, 610
BstYI RGATCY 5 cut(s) 305, 360, 481, 521, 610
BstZI CGGCCG 1 cut(s) 154
BtgI CCRYGG 2 cut(s) 157, 333
BtgZI GCGATG 1 cut(s) 1166
BtsCI GGATG 4 cut(s) 343, 358, 999, 1100
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 2 cut(s) 190, 1023
Cac8I GCNNGC 4 cut(s) 196, 402, 903, 969
CaiI CAGNNNCTG 1 cut(s) 117
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 2 cut(s) 140, 406
Cfr10I RCCGGY 3 cut(s) 190, 901, 952
Cfr13I GGNCC 7 cut(s) 161, 297, 320, 464, 810, 892, 893
Cfr42I CCGCGG 2 cut(s) 160, 336
CseI GACGC 1 cut(s) 255
Csp6I GTAC 2 cut(s) 585, 1137
CviAII CATG 5 cut(s) 526, 544, 620, 1010, 1132
CviQI GTAC 2 cut(s) 585, 1137
DdeI CTNAG 6 cut(s) 45, 283, 485, 721, 909, 1038
DinI GGCGCC 1 cut(s) 405
DpnI GATC 7 cut(s) 307, 362, 417, 483, 523, 591, 612
DpnII GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
DraIII CACNNNGTG 1 cut(s) 211
EaeI YGGCCR 4 cut(s) 28, 154, 365, 957
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 5 cut(s) 223, 250, 851, 980, 1174
EarI CTCTTC 5 cut(s) 223, 250, 851, 980, 1174
EciI GGCGGA 2 cut(s) 656, 1058
Ecl136II GAGCTC 3 cut(s) 7, 330, 1037
EclXI CGGCCG 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 918
Eco24I GRGCYC 5 cut(s) 9, 200, 332, 896, 1039
Eco47I GGWCC 1 cut(s) 297
Eco47III AGCGCT 1 cut(s) 139
Eco52I CGGCCG 1 cut(s) 154
Eco53kI GAGCTC 3 cut(s) 7, 330, 1037
Eco57I CTGAAG 2 cut(s) 776, 924
EcoICRI GAGCTC 3 cut(s) 7, 330, 1037
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 918
EcoT38I GRGCYC 5 cut(s) 9, 200, 332, 896, 1039
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 918
FaeI CATG 5 cut(s) 529, 547, 623, 1013, 1135
FaiI YATR 6 cut(s) 527, 545, 621, 1011, 1122, 1133
FalI AAGNNNNNCTT 4 cut(s) 1052, 1084, 1074, 1106
FaqI GGGAC 1 cut(s) 509
FatI CATG 5 cut(s) 525, 543, 619, 1009, 1131
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 380
FokI GGATG 4 cut(s) 350, 365, 1006, 1107
FriOI GRGCYC 5 cut(s) 9, 200, 332, 896, 1039
FspBI CTAG 3 cut(s) 63, 309, 519
GlaI GCGC 2 cut(s) 139, 405
GsuI CTGGAG 2 cut(s) 130, 410
HaeII RGCGCY 2 cut(s) 141, 407
HgaI GACGC 1 cut(s) 255
HhaI GCGC 2 cut(s) 140, 406
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 5 cut(s) 529, 547, 623, 1013, 1135
Hin6I GCGC 2 cut(s) 138, 404
HinP1I GCGC 2 cut(s) 138, 404
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 3 cut(s) 291, 865, 907
Hpy166II GTNNAC 2 cut(s) 188, 381
Hpy188I TCNGA 4 cut(s) 271, 486, 756, 912
Hpy188III TCNNGA 3 cut(s) 614, 828, 1010
Hpy8I GTNNAC 2 cut(s) 188, 381
Hpy99I CGWCG 2 cut(s) 271, 1006
HpyAV CCTTC 6 cut(s) 631, 699, 751, 779, 907, 1108
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 188, 205, 421, 1018
HpyF3I CTNAG 6 cut(s) 45, 283, 485, 721, 909, 1038
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 5 cut(s) 529, 547, 623, 1013, 1135
HspAI GCGC 2 cut(s) 138, 404
KasI GGCGCC 1 cut(s) 403
KroI GCCGGC 1 cut(s) 901
KroNI GCCGGC 1 cut(s) 903
Ksp22I TGATCA 1 cut(s) 589
KspI CCGCGG 2 cut(s) 160, 336
Kzo9I GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
LmnI GCTCC 8 cut(s) 4, 125, 145, 335, 391, 672, 898, 1124
Lsp1109I GCAGC 5 cut(s) 396, 406, 492, 1002, 1114
LweI GCATC 1 cut(s) 1085
MaeI CTAG 3 cut(s) 63, 309, 519
MaeII ACGT 1 cut(s) 132
MalI GATC 7 cut(s) 307, 362, 417, 483, 523, 591, 612
MboI GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 5 cut(s) 305, 360, 481, 521, 610
MhlI GDGCHC 8 cut(s) 9, 190, 200, 216, 332, 896, 1039, 1156
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 2 cut(s) 803, 927
MluNI TGGCCA 1 cut(s) 30
Mly113I GGCGCC 1 cut(s) 404
MlyI GAGTC 2 cut(s) 874, 901
MmeI TCCRAC 2 cut(s) 45, 249
Mox20I TGGCCA 1 cut(s) 30
MroNI GCCGGC 1 cut(s) 901
MscI TGGCCA 1 cut(s) 30
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 4 cut(s) 159, 335, 409, 725
MspR9I CCNGG 4 cut(s) 153, 319, 389, 896
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 3 cut(s) 159, 266, 335
NaeI GCCGGC 1 cut(s) 903
NarI GGCGCC 1 cut(s) 404
NciI CCSGG 3 cut(s) 153, 319, 896
NdeII GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
NgoMIV GCCGGC 1 cut(s) 901
NlaIII CATG 5 cut(s) 529, 547, 623, 1013, 1135
NlaIV GGNNCC 6 cut(s) 163, 298, 405, 894, 900, 999
NspI RCATGY 1 cut(s) 547
PagI TCATGA 1 cut(s) 1009
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PdiI GCCGGC 1 cut(s) 903
PfeI GAWTC 1 cut(s) 291
PflMI CCANNNNNTGG 2 cut(s) 620, 974
PleI GAGTC 2 cut(s) 873, 901
PluTI GGCGCC 1 cut(s) 407
PpsI GAGTC 2 cut(s) 873, 901
Psp124BI GAGCTC 3 cut(s) 9, 332, 1039
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 6 cut(s) 163, 298, 405, 894, 900, 999
PspOMI GGGCCC 1 cut(s) 892
PspPI GGNCC 7 cut(s) 161, 297, 320, 464, 810, 892, 893
PstNI CAGNNNCTG 1 cut(s) 117
PsuI RGATCY 5 cut(s) 305, 360, 481, 521, 610
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 2 cut(s) 586, 1138
RsaNI GTAC 2 cut(s) 585, 1137
SacI GAGCTC 3 cut(s) 9, 332, 1039
SacII CCGCGG 2 cut(s) 160, 336
SalI GTCGAC 1 cut(s) 379
Sau3AI GATC 7 cut(s) 305, 360, 415, 481, 521, 589, 610
Sau96I GGNCC 7 cut(s) 161, 297, 320, 464, 810, 892, 893
ScaI AGTACT 2 cut(s) 586, 1138
SchI GAGTC 2 cut(s) 874, 901
ScrFI CCNGG 4 cut(s) 153, 319, 389, 896
SduI GDGCHC 8 cut(s) 9, 190, 200, 216, 332, 896, 1039, 1156
SfaNI GCATC 1 cut(s) 1085
SfcI CTRYAG 2 cut(s) 181, 1075
SfoI GGCGCC 1 cut(s) 405
Sfr303I CCGCGG 2 cut(s) 160, 336
SgrAI CRCCGGYG 1 cut(s) 190
SgrBI CCGCGG 2 cut(s) 160, 336
SinI GGWCC 1 cut(s) 297
Sse9I AATT 2 cut(s) 803, 927
SspDI GGCGCC 1 cut(s) 403
SspMI CTAG 3 cut(s) 63, 309, 519
SstI GAGCTC 3 cut(s) 9, 332, 1039
StyD4I CCNGG 4 cut(s) 151, 317, 387, 894
StyI CCWWGG 1 cut(s) 918
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 1 cut(s) 135
TasI AATT 2 cut(s) 803, 927
TatI WGTACW 2 cut(s) 584, 1136
TauI GCSGC 5 cut(s) 159, 162, 400, 409, 926
TfiI GAWTC 1 cut(s) 291
TscAI CASTG 2 cut(s) 190, 1030
TseI GCWGC 5 cut(s) 394, 409, 505, 1015, 1127
TspDTI ATGAA 5 cut(s) 245, 386, 591, 869, 998
TspRI CASTG 2 cut(s) 190, 1030
Van91I CCANNNNNTGG 2 cut(s) 620, 974
VneI GTGCAC 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 1 cut(s) 803
XceI RCATGY 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 380
XspI CTAG 3 cut(s) 63, 309, 519
ZrmI AGTACT 2 cut(s) 586, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.