MD12G1207500.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002353
Physical Location & Seq
Reverse (-)
28732569 .. 28733816
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1207500.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGAGCTCAAAACTTCACCCGGCAATGGCACCCAACCCCCAAACCCTGAACCGAAACCCGACCCTCCAAACCCTAGCAATGGCGAAACCCAGCTTCAAGACCAGCTTTTCTTTGAAGACTACGACAGCACCTGCTCCACGCCTTACGTCAGCGCGCCGTCGAGCCCCGGACGGAGTGGGCCGGTCAGTGGTTTTTTCTACAGTGCTCCGGCGAGCCCAATGCACTTCGGGTTGAGCACAAAGTCAAACTCTTGTTCGCTTGAGAAGAATGGCGGCAACGGCAGCGGCTCTGTTTCTATGGGTTTTGAGTTCGAGTTCTCTGCGCGGTTCGGGTCGAGCGGGTCGGGCTCCACCGGTCCGATGAGCTCTGCCGACGAGTTGTTCTTGAACGGGAAGATCCGACCCATGAAACTTTCGACCCACCTGGAAAAGCCCCAGACTTTGGCTCCGTTGTTGGATCTCGAGGCGGAAAATGGGGAAGACGAGGCGGATCGGGGCGGTTTGGAGCCCGTGAGAGGCAGAGATCTGAGGTTCCGCGACAAGTCGCTGCGGCGGAGGACGAGATCTATGTCCCCTCTGAGAAATGAGCCCTTCGAGTGGGAACAGAGGGACGAGATTATTGAAGCTGATGAGAAGGACTGCAACTTTGCTGAAGATTCGAAAGCGCCGGCGAAAAATGGGGAAGAAGACGAAGAGGGAGCGGAGGAGAGCAGCACCGCCACTACTACTCCTTCGGTTTCGGCTTCGTCGTCTCGGTCGTCGTCGGGTGGGAGGAACTCGAAGAAGTGGGTTTTTTTGAAGGAGTTTCTTCACCGGAGTAAAAGCGAAGGGCGGAGCAGCAACCACAAGTTCTGGTCGTCGATTTCGTTCTCCGCCGCGAAGGAGAAGAAACCCACGAAACAGGGCGGTCAGAATACCCAGGGCTCTGCTCTAAAAGACCCCAAATGTAGCAATCCTTTGAACTTGGGCTCAGAGGGGCAAAAACTGAAGGGAAGTGGGGGTGGTGGTTCGGGGAAGAAGACGGCGGCCGTAAAACCCACCAACGGAGTTGGTAAGCGGCGGATGGCGCCGTCGCTTCACGAGCTGCATTACACTGCAAACAGGGCTCAGGCAGAGGAAATGAGGAAGAAGACTTACTTGCCTTACAGGCAAGGGTTGCTTGGATGCTTAGGGTTTAGCTCAAAGGGTTATGGAGCTGCCATGAACTCTGGGTTTGCTAGGGCTTTGAATCCTGTTTCTTCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

416

Amino Acids

44.63

Weight (kDa)

8.9

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 124 - 381 2.3e-42 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 140
AccB1I GGYRCC 2 cut(s) 29, 1066
AccB7I CCANNNNNTGG 1 cut(s) 442
AccBSI CCGCTC 2 cut(s) 339, 701
AccII CGCG 4 cut(s) 155, 325, 537, 878
AclWI GGATC 3 cut(s) 391, 465, 498
AcoI YGGCCR 1 cut(s) 1026
AcuI CTGAAG 2 cut(s) 672, 1007
AcyI GRCGYC 1 cut(s) 1067
AfiI CCNNNNNNNGG 7 cut(s) 26, 80, 188, 442, 515, 597, 1043
AgeI ACCGGT 1 cut(s) 353
AgsI TTSAA 8 cut(s) 98, 116, 388, 623, 799, 961, 1228, 1242
AjnI CCWGG 2 cut(s) 423, 918
AluBI AGCT 8 cut(s) 7, 94, 106, 366, 626, 1084, 1179, 1196
AluI AGCT 8 cut(s) 7, 94, 106, 366, 626, 1084, 1179, 1196
Alw21I GWGCWC 4 cut(s) 9, 208, 239, 368
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 3 cut(s) 391, 465, 498
AlwNI CAGNNNCTG 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 2 cut(s) 179, 1026
ApeKI GCWGC 6 cut(s) 282, 547, 711, 837, 1084, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AsiGI ACCGGT 1 cut(s) 353
AspLEI GCGC 5 cut(s) 155, 157, 325, 667, 1069
AspS9I GGNCC 2 cut(s) 179, 356
AsuC2I CCSGG 2 cut(s) 21, 168
AsuHPI GGTGA 2 cut(s) 9, 803
AsuII TTCGAA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 356
BanI GGYRCC 2 cut(s) 29, 1066
BarI GAAGNNNNNNTAC 2 cut(s) 1118, 1150
BauI CACGAG 1 cut(s) 1079
BbsI GAAGAC 5 cut(s) 123, 486, 693, 1025, 1136
Bbv12I GWGCWC 4 cut(s) 9, 208, 239, 368
BbvI GCAGC 6 cut(s) 294, 534, 723, 849, 1071, 1183
BccI CCATC 1 cut(s) 1057
BceAI ACGGC 5 cut(s) 142, 295, 1013, 1038, 1054
BcgI CGANNNNNNTGC 4 cut(s) 202, 236, 302, 336
BciT130I CCWGG 2 cut(s) 425, 920
BcnI CCSGG 2 cut(s) 21, 168
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 2 cut(s) 75, 1218
BfmI CTRYAG 1 cut(s) 199
BfoI RGCGCY 2 cut(s) 668, 1070
BfuAI ACCTGC 1 cut(s) 140
BglI GCCNNNNNGGC 1 cut(s) 1147
BglII AGATCT 2 cut(s) 523, 563
Bme1390I CCNGG 4 cut(s) 21, 168, 425, 920
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 179, 356
BmiI GGNNCC 6 cut(s) 31, 349, 447, 507, 533, 1068
BmrFI CCNGG 4 cut(s) 21, 168, 425, 920
BmsI GCATC 1 cut(s) 1154
BpiI GAAGAC 5 cut(s) 123, 486, 693, 1025, 1136
Bpu10I CCTNAGC 2 cut(s) 1107, 1168
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 21, 168
BsaHI GRCGYC 1 cut(s) 1067
BsaJI CCNNGG 3 cut(s) 166, 918, 919
BsaWI WCCGGW 2 cut(s) 353, 813
BsaXI ACNNNNNCTCC 4 cut(s) 430, 460, 712, 742
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 80, 188, 442, 515, 597, 1043
Bse118I RCCGGY 3 cut(s) 181, 353, 667
Bse3DI GCAATG 2 cut(s) 31, 85
BseBI CCWGG 2 cut(s) 425, 920
BseDI CCNNGG 3 cut(s) 166, 918, 919
BseGI GGATG 2 cut(s) 1068, 1169
BseLI CCNNNNNNNGG 7 cut(s) 26, 80, 188, 442, 515, 597, 1043
BseMI GCAATG 2 cut(s) 31, 85
BseMII CTCAG 4 cut(s) 518, 569, 984, 1121
BsePI GCGCGC 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 719
BseX3I CGGCCG 1 cut(s) 1026
BseXI GCAGC 6 cut(s) 294, 534, 723, 849, 1071, 1183
BseYI CCCAGC 1 cut(s) 90
Bsh1236I CGCG 4 cut(s) 155, 325, 537, 878
Bsh1285I CGRYCG 2 cut(s) 758, 1029
BshFI GGCC 2 cut(s) 181, 1028
BshNI GGYRCC 2 cut(s) 29, 1066
BshTI ACCGGT 1 cut(s) 353
BsiEI CGRYCG 2 cut(s) 758, 1029
BsiHKAI GWGCWC 4 cut(s) 9, 208, 239, 368
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 7 cut(s) 21, 168, 182, 209, 354, 668, 814
BslFI GGGAC 2 cut(s) 556, 623
BslI CCNNNNNNNGG 7 cut(s) 26, 80, 188, 442, 515, 597, 1043
BsmAI GTCTC 1 cut(s) 756
BsmBI CGTCTC 1 cut(s) 756
BsmFI GGGAC 2 cut(s) 556, 623
BsnI GGCC 2 cut(s) 181, 1028
BsoBI CYCGRG 1 cut(s) 461
Bsp119I TTCGAA 1 cut(s) 659
Bsp143I GATC 5 cut(s) 396, 457, 490, 523, 563
BspANI GGCC 2 cut(s) 181, 1028
BspCNI CTCAG 4 cut(s) 519, 570, 983, 1120
BspFNI CGCG 4 cut(s) 155, 325, 537, 878
BspLI GGNNCC 6 cut(s) 31, 349, 447, 507, 533, 1068
BspMI ACCTGC 1 cut(s) 140
BspPI GGATC 3 cut(s) 391, 465, 498
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 2 cut(s) 29, 1066
BsrBI CCGCTC 2 cut(s) 339, 701
BsrDI GCAATG 2 cut(s) 31, 85
BsrFI RCCGGY 3 cut(s) 181, 353, 667
BssAI RCCGGY 3 cut(s) 181, 353, 667
BssECI CCNNGG 3 cut(s) 166, 918, 919
BssHII GCGCGC 1 cut(s) 153
BssMI GATC 5 cut(s) 396, 457, 490, 523, 563
BssNI GRCGYC 1 cut(s) 1067
BssSI CACGAG 1 cut(s) 1079
Bst2BI CACGAG 1 cut(s) 1079
Bst2UI CCWGG 2 cut(s) 425, 920
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 1067
BstAPI GCANNNNNTGC 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 3 cut(s) 155, 214, 669
BstDEI CTNAG 5 cut(s) 527, 578, 970, 1107, 1168
BstF5I GGATG 2 cut(s) 1068, 1169
BstFNI CGCG 4 cut(s) 155, 325, 537, 878
BstH2I RGCGCY 2 cut(s) 668, 1070
BstHHI GCGC 5 cut(s) 155, 157, 325, 667, 1069
BstKTI GATC 5 cut(s) 399, 460, 493, 526, 566
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 5 cut(s) 396, 457, 490, 523, 563
BstMCI CGRYCG 2 cut(s) 758, 1029
BstMWI GCNNNNNNNGC 8 cut(s) 279, 282, 345, 1066, 1081, 1103, 1147, 1156
BstNI CCWGG 2 cut(s) 425, 920
BstSCI CCNGG 4 cut(s) 19, 166, 423, 918
BstSFI CTRYAG 1 cut(s) 199
BstUI CGCG 4 cut(s) 155, 325, 537, 878
BstV1I GCAGC 6 cut(s) 294, 534, 723, 849, 1071, 1183
BstV2I GAAGAC 5 cut(s) 123, 486, 693, 1025, 1136
BstX2I RGATCY 4 cut(s) 396, 457, 523, 563
BstYI RGATCY 4 cut(s) 396, 457, 523, 563
BstZI CGGCCG 1 cut(s) 1026
BsuRI GGCC 2 cut(s) 181, 1028
BtsCI GGATG 2 cut(s) 1068, 1169
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 3 cut(s) 193, 208, 1092
BveI ACCTGC 1 cut(s) 140
Cac8I GCNNGC 3 cut(s) 155, 214, 669
CaiI CAGNNNCTG 1 cut(s) 132
CfoI GCGC 5 cut(s) 155, 157, 325, 667, 1069
Cfr10I RCCGGY 3 cut(s) 181, 353, 667
Cfr13I GGNCC 2 cut(s) 179, 356
CpoI CGGWCCG 1 cut(s) 356
CspAI ACCGGT 1 cut(s) 353
CspI CGGWCCG 1 cut(s) 356
CviAII CATG 2 cut(s) 406, 1201
DdeI CTNAG 5 cut(s) 527, 578, 970, 1107, 1168
DinI GGCGCC 1 cut(s) 1068
DpnI GATC 5 cut(s) 398, 459, 492, 525, 565
DpnII GATC 5 cut(s) 396, 457, 490, 523, 563
EaeI YGGCCR 1 cut(s) 1026
EagI CGGCCG 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
EciI GGCGGA 6 cut(s) 482, 503, 568, 847, 862, 1075
Ecl136II GAGCTC 2 cut(s) 7, 366
EclXI CGGCCG 1 cut(s) 1026
Eco47I GGWCC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 1026
Eco53kI GAGCTC 2 cut(s) 7, 366
Eco57I CTGAAG 2 cut(s) 672, 1007
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 2 cut(s) 7, 366
EcoRII CCWGG 2 cut(s) 423, 918
EgeI GGCGCC 1 cut(s) 1068
EheI GGCGCC 1 cut(s) 1068
Esp3I CGTCTC 1 cut(s) 756
FaeI CATG 2 cut(s) 409, 1204
FaiI YATR 5 cut(s) 299, 407, 569, 1191, 1202
FalI AAGNNNNNCTT 6 cut(s) 90, 122, 1121, 1153, 1143, 1175
FaqI GGGAC 2 cut(s) 556, 623
FatI CATG 2 cut(s) 405, 1200
FauI CCCGC 1 cut(s) 332
FokI GGATG 2 cut(s) 1075, 1176
FspBI CTAG 2 cut(s) 75, 1218
GlaI GCGC 5 cut(s) 154, 156, 324, 666, 1068
GsaI CCCAGC 1 cut(s) 94
HaeII RGCGCY 2 cut(s) 668, 1070
HaeIII GGCC 2 cut(s) 181, 1028
HapII CCGG 7 cut(s) 21, 168, 182, 209, 354, 668, 814
HhaI GCGC 5 cut(s) 155, 157, 325, 667, 1069
Hin1I GRCGYC 1 cut(s) 1067
Hin1II CATG 2 cut(s) 409, 1204
Hin6I GCGC 5 cut(s) 153, 155, 323, 665, 1067
HinP1I GCGC 5 cut(s) 153, 155, 323, 665, 1067
HinfI GANTC 2 cut(s) 656, 1228
HpaII CCGG 7 cut(s) 21, 168, 182, 209, 354, 668, 814
HphI GGTGA 2 cut(s) 9, 803
Hpy188I TCNGA 6 cut(s) 360, 401, 528, 579, 912, 973
Hpy188III TCNNGA 4 cut(s) 98, 385, 461, 1079
Hpy99I CGWCG 7 cut(s) 163, 377, 751, 763, 766, 862, 1075
HpyAV CCTTC 7 cut(s) 601, 628, 741, 793, 821, 874, 982
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 4 cut(s) 223, 642, 1087, 1097
HpyF10VI GCNNNNNNNGC 8 cut(s) 279, 282, 345, 1066, 1081, 1103, 1147, 1156
HpyF3I CTNAG 5 cut(s) 527, 578, 970, 1107, 1168
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 1067
Hsp92II CATG 2 cut(s) 409, 1204
HspAI GCGC 5 cut(s) 153, 155, 323, 665, 1067
KasI GGCGCC 1 cut(s) 1066
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Kzo9I GATC 5 cut(s) 396, 457, 490, 523, 563
LmnI GCTCC 9 cut(s) 4, 140, 211, 353, 451, 505, 698, 834, 1193
Lsp1109I GCAGC 6 cut(s) 294, 534, 723, 849, 1071, 1183
LweI GCATC 1 cut(s) 1154
MaeI CTAG 2 cut(s) 75, 1218
MaeII ACGT 1 cut(s) 147
MalI GATC 5 cut(s) 398, 459, 492, 525, 565
MbiI CCGCTC 2 cut(s) 339, 701
MboI GATC 5 cut(s) 396, 457, 490, 523, 563
MflI RGATCY 4 cut(s) 396, 457, 523, 563
Mly113I GGCGCC 1 cut(s) 1067
MmeI TCCRAC 2 cut(s) 424, 435
MreI CGCCGGCG 1 cut(s) 667
MroNI GCCGGC 1 cut(s) 667
MspA1I CMGCKG 1 cut(s) 285
MspI CCGG 7 cut(s) 21, 168, 182, 209, 354, 668, 814
MspR9I CCNGG 4 cut(s) 21, 168, 425, 920
MvaI CCWGG 2 cut(s) 425, 920
MvnI CGCG 4 cut(s) 155, 325, 537, 878
MwoI GCNNNNNNNGC 8 cut(s) 279, 282, 345, 1066, 1081, 1103, 1147, 1156
NaeI GCCGGC 1 cut(s) 669
NarI GGCGCC 1 cut(s) 1067
NciI CCSGG 2 cut(s) 21, 168
NdeII GATC 5 cut(s) 396, 457, 490, 523, 563
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 2 cut(s) 409, 1204
NlaIV GGNNCC 6 cut(s) 31, 349, 447, 507, 533, 1068
NspV TTCGAA 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 461
PaqCI CACCTGC 1 cut(s) 140
PasI CCCWGGG 1 cut(s) 919
PauI GCGCGC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 3 cut(s) 746, 755, 863
PdiI GCCGGC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 656, 1228
PflMI CCANNNNNTGG 1 cut(s) 442
PinAI ACCGGT 1 cut(s) 353
PluTI GGCGCC 1 cut(s) 1070
Psp124BI GAGCTC 2 cut(s) 9, 368
Psp6I CCWGG 2 cut(s) 423, 918
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 2 cut(s) 423, 918
PspN4I GGNNCC 6 cut(s) 31, 349, 447, 507, 533, 1068
PspPI GGNCC 2 cut(s) 179, 356
PstNI CAGNNNCTG 1 cut(s) 132
PsuI RGATCY 4 cut(s) 396, 457, 523, 563
PteI GCGCGC 1 cut(s) 153
Rsr2I CGGWCCG 1 cut(s) 356
RsrII CGGWCCG 1 cut(s) 356
SacI GAGCTC 2 cut(s) 9, 368
Sau3AI GATC 5 cut(s) 396, 457, 490, 523, 563
Sau96I GGNCC 2 cut(s) 179, 356
ScrFI CCNGG 4 cut(s) 21, 168, 425, 920
SfaNI GCATC 1 cut(s) 1154
SfcI CTRYAG 1 cut(s) 199
SfoI GGCGCC 1 cut(s) 1068
Sfr274I CTCGAG 1 cut(s) 461
SfuI TTCGAA 1 cut(s) 659
SgrAI CRCCGGYG 1 cut(s) 667
SinI GGWCC 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 461
SmlI CTYRAG 2 cut(s) 260, 461
SmoI CTYRAG 2 cut(s) 260, 461
SspDI GGCGCC 1 cut(s) 1066
SspMI CTAG 2 cut(s) 75, 1218
SstI GAGCTC 2 cut(s) 9, 368
StyD4I CCNGG 4 cut(s) 19, 166, 423, 918
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 1 cut(s) 150
TaqI TCGA 9 cut(s) 161, 312, 335, 416, 462, 594, 659, 779, 860
TaqII GACCGA 1 cut(s) 744
TauI GCSGC 6 cut(s) 276, 288, 553, 878, 1028, 1060
TfiI GAWTC 2 cut(s) 656, 1228
TscAI CASTG 3 cut(s) 193, 208, 1099
TseI GCWGC 6 cut(s) 282, 547, 711, 837, 1084, 1196
TspDTI ATGAA 2 cut(s) 422, 1217
TspGWI ACGGA 3 cut(s) 187, 438, 1059
TspRI CASTG 3 cut(s) 193, 208, 1099
Van91I CCANNNNNTGG 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 356
XhoI CTCGAG 1 cut(s) 461
XspI CTAG 2 cut(s) 75, 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.