MD04G1195400.v1.1

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002353
Physical Location & Seq
Reverse (-)
28407643 .. 28408899
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1195400.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGGAGCTCAAAACTTCGCCCGGCAATGGTACCCAACCCCCAAACCCTGAACCGAAACCCGACCCGTCAAACCCTGGTAATGCCGAAACCCAGCAAACCCAGCTAGAAAACCAGCTCTTCTTTGAAGACTACGACAGCACCTGCTCCACACCTTACGTCAGCGCGCCGTCGAGCCCCGGACGGAGTGGGCCGGTCAGTGGGTTTTTCTACAGTGCTCCGGCGAGCCCAATGCACTTCGCATTGACCACAAAGTCGAACTCATCTTCGTTTGAGAAGAATGGCGGGAACGGCAGCGACTCTGTTTCTATGGGTTTTGAGTTCGAGTTCTCTGCGCGGTTCGGGTCGAGCGGGTCCGGCCCGACCGGTCCGATGAGCTCGGCCGACGAGTTGTTCTTGAACGGCAAGATCCGACCCATGAAGCTCTCGACCCACCTGGAAAAGCCCCAGACTTTGGCTCCGTTGCTGGATCTCGAACCGGAAACTGGGGAAGACGAAGCGGATCGGGTGGGGTTCGAACCCGTGAGAGGCAGAGATCTGAGGTTCCGTGACAAGTCCCTGCGGCGGAGGACGAGATCTTTGTCCCCTTTGAGACATGAGCCATTCGAGTGGGGACAGAACGACGAGATTCTTGAAGCTGAAGAGAAAGACTGCAACTTTGCTGAAGATCCGAAAGGGGCGGCGAAAAACGGGGAAGAAGACGAAGAGGGAGTGGAGGAGAGCAGCAGCACCACCACTACTCCTTCGGTTTCAGCTTCGTCGTCGCGGTCGTCGTCGGTGGGGCGGAACTCGAAAAGGTGGGTTTTTCTGAAGGAGTTTCTTCACCGGAGTAAGAGTGAAGGGCGGAGCAGCAACCACAAGTTCTGGTCGTCGATTTCGTTCTCCGCCGCGAAAGAGAAGAAACCCATGAAACAGGGCGGTCAGAATGCTCAGGGCTCTGTTTCAAAAGACCCCAAATGCAGCAACCCTTTGAACTTGGGCTCAGAGGGGCAAAAATTGAAGGGAAGTGGGAGCGGTGGTTCGGGGAAGAAGACGGGGGCCGGAAAACCCACCAACGGTGTCGGGAAGCGGCGTCATCCGCCGTCGCTTCACGAGCTGCATTACACTGCAAACAGGGCTCAGGCTGAGGAAATGAGGAAAAAGACTTACTTGCCTTACAGGCAAGGGTTGCTCGGATGCTTAGGGTTTAGCTCAAAGGGTTATGGAGCTGCCATGAATTCTGGGTTTGCTAGGGCTTTGAATCCAGTTTCTTCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

419

Amino Acids

45.16

Weight (kDa)

8.84

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 127 - 384 1.2e-42 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
AasI GACNNNNNNGTC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 149
Acc65I GGTACC 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 451
AccBSI CCGCTC 2 cut(s) 348, 1011
AccII CGCG 4 cut(s) 164, 334, 763, 887
AclWI GGATC 4 cut(s) 400, 474, 507, 659
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 1 cut(s) 1213
AcuI CTGAAG 3 cut(s) 657, 681, 827
AcyI GRCGYC 1 cut(s) 1069
AfaI GTAC 1 cut(s) 31
AfiI CCNNNNNNNGG 7 cut(s) 26, 197, 451, 482, 524, 561, 1052
AgeI ACCGGT 1 cut(s) 362
AgsI TTSAA 8 cut(s) 125, 397, 632, 942, 970, 997, 1237, 1251
AjnI CCWGG 2 cut(s) 73, 432
AloI GAACNNNNNNTCC 2 cut(s) 1000, 1032
Alw21I GWGCWC 3 cut(s) 9, 217, 377
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 4 cut(s) 400, 474, 507, 659
AlwNI CAGNNNCTG 1 cut(s) 141
AoxI GGCC 4 cut(s) 188, 355, 378, 1035
ApeKI GCWGC 7 cut(s) 291, 720, 723, 846, 957, 1093, 1205
ApoI RAATTY 1 cut(s) 1213
ArsI GACNNNNNNTTYG 4 cut(s) 559, 591, 638, 670
AsiGI ACCGGT 1 cut(s) 362
Asp718I GGTACC 1 cut(s) 29
AspLEI GCGC 3 cut(s) 164, 166, 334
AspS9I GGNCC 5 cut(s) 188, 351, 356, 365, 1035
AsuC2I CCSGG 2 cut(s) 21, 177
AsuHPI GGTGA 1 cut(s) 812
AsuII TTCGAA 1 cut(s) 513
AvaII GGWCC 2 cut(s) 351, 365
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 7 cut(s) 9, 176, 227, 377, 935, 980, 1117
BauI CACGAG 1 cut(s) 1088
BbsI GAAGAC 4 cut(s) 132, 495, 702, 1034
Bbv12I GWGCWC 3 cut(s) 9, 217, 377
BbvCI CCTCAGC 1 cut(s) 1122
BbvI GCAGC 7 cut(s) 303, 732, 735, 858, 969, 1080, 1192
BceAI ACGGC 4 cut(s) 151, 304, 415, 1063
BcgI CGANNNNNNTGC 4 cut(s) 211, 245, 311, 345
BciT130I CCWGG 2 cut(s) 75, 434
BcnI CCSGG 2 cut(s) 21, 177
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 2 cut(s) 104, 1227
BfmI CTRYAG 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 1156
BglII AGATCT 2 cut(s) 532, 572
Bme1390I CCNGG 4 cut(s) 21, 75, 177, 434
Bme18I GGWCC 2 cut(s) 351, 365
BmgT120I GGNCC 5 cut(s) 188, 351, 356, 365, 1035
BmiI GGNNCC 5 cut(s) 31, 352, 456, 542, 1036
BmrFI CCNGG 4 cut(s) 21, 75, 177, 434
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 1 cut(s) 1163
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 4 cut(s) 132, 495, 702, 1034
Bpu10I CCTNAGC 4 cut(s) 927, 1116, 1122, 1177
Bpu14I TTCGAA 1 cut(s) 513
BpuMI CCSGG 2 cut(s) 21, 177
BsaHI GRCGYC 1 cut(s) 1069
BsaJI CCNNGG 2 cut(s) 73, 175
BsaWI WCCGGW 3 cut(s) 362, 475, 822
BsaXI ACNNNNNCTCC 8 cut(s) 439, 469, 721, 751, 817, 847, 1000, 1030
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 197, 451, 482, 524, 561, 1052
Bse118I RCCGGY 2 cut(s) 190, 362
Bse1I ACTGG 2 cut(s) 487, 1241
Bse3DI GCAATG 1 cut(s) 31
BseBI CCWGG 2 cut(s) 75, 434
BseDI CCNNGG 2 cut(s) 73, 175
BseGI GGATG 2 cut(s) 1072, 1178
BseLI CCNNNNNNNGG 7 cut(s) 26, 197, 451, 482, 524, 561, 1052
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 5 cut(s) 527, 941, 993, 1113, 1130
BseNI ACTGG 2 cut(s) 487, 1241
BsePI GCGCGC 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 728
BseX3I CGGCCG 1 cut(s) 378
BseXI GCAGC 7 cut(s) 303, 732, 735, 858, 969, 1080, 1192
BseYI CCCAGC 2 cut(s) 90, 99
Bsh1236I CGCG 4 cut(s) 164, 334, 763, 887
Bsh1285I CGRYCG 3 cut(s) 363, 381, 767
BshFI GGCC 4 cut(s) 190, 357, 380, 1037
BshNI GGYRCC 1 cut(s) 29
BshTI ACCGGT 1 cut(s) 362
BsiEI CGRYCG 3 cut(s) 363, 381, 767
BsiHKAI GWGCWC 3 cut(s) 9, 217, 377
BsiSI CCGG 9 cut(s) 21, 177, 191, 218, 354, 363, 476, 823, 1038
BslFI GGGAC 3 cut(s) 538, 565, 624
BslI CCNNNNNNNGG 7 cut(s) 26, 197, 451, 482, 524, 561, 1052
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 3 cut(s) 538, 565, 624
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 4 cut(s) 190, 357, 380, 1037
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 8 cut(s) 9, 176, 217, 227, 377, 935, 980, 1117
Bsp143I GATC 6 cut(s) 405, 466, 499, 532, 572, 664
BspANI GGCC 4 cut(s) 190, 357, 380, 1037
BspCNI CTCAG 5 cut(s) 528, 940, 992, 1114, 1129
BspFNI CGCG 4 cut(s) 164, 334, 763, 887
BspLI GGNNCC 5 cut(s) 31, 352, 456, 542, 1036
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 4 cut(s) 400, 474, 507, 659
BspQI GCTCTTC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 2 cut(s) 348, 1011
BsrDI GCAATG 1 cut(s) 31
BsrFI RCCGGY 2 cut(s) 190, 362
BsrI ACTGG 2 cut(s) 487, 1241
BssAI RCCGGY 2 cut(s) 190, 362
BssECI CCNNGG 2 cut(s) 73, 175
BssHII GCGCGC 1 cut(s) 162
BssMI GATC 6 cut(s) 405, 466, 499, 532, 572, 664
BssNI GRCGYC 1 cut(s) 1069
BssSI CACGAG 1 cut(s) 1088
Bst2BI CACGAG 1 cut(s) 1088
Bst2UI CCWGG 2 cut(s) 75, 434
Bst4CI ACNGT 2 cut(s) 212, 1055
Bst6I CTCTTC 3 cut(s) 122, 633, 696
BstACI GRCGYC 1 cut(s) 1069
BstAPI GCANNNNNTGC 1 cut(s) 1165
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 164, 223
BstDEI CTNAG 6 cut(s) 536, 927, 979, 1116, 1122, 1177
BstF5I GGATG 2 cut(s) 1072, 1178
BstFNI CGCG 4 cut(s) 164, 334, 763, 887
BstHHI GCGC 3 cut(s) 164, 166, 334
BstKTI GATC 6 cut(s) 408, 469, 502, 535, 575, 667
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 6 cut(s) 405, 466, 499, 532, 572, 664
BstMCI CGRYCG 3 cut(s) 363, 381, 767
BstMWI GCNNNNNNNGC 8 cut(s) 100, 288, 354, 1075, 1090, 1112, 1156, 1165
BstNI CCWGG 2 cut(s) 75, 434
BstSCI CCNGG 4 cut(s) 19, 73, 175, 432
BstSFI CTRYAG 1 cut(s) 208
BstUI CGCG 4 cut(s) 164, 334, 763, 887
BstV1I GCAGC 7 cut(s) 303, 732, 735, 858, 969, 1080, 1192
BstV2I GAAGAC 4 cut(s) 132, 495, 702, 1034
BstX2I RGATCY 5 cut(s) 405, 466, 532, 572, 664
BstXI CCANNNNNNTGG 1 cut(s) 606
BstYI RGATCY 5 cut(s) 405, 466, 532, 572, 664
BstZI CGGCCG 1 cut(s) 378
BsuRI GGCC 4 cut(s) 190, 357, 380, 1037
BtsCI GGATG 2 cut(s) 1072, 1178
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 3 cut(s) 202, 217, 1101
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 2 cut(s) 164, 223
CaiI CAGNNNCTG 1 cut(s) 141
CfoI GCGC 3 cut(s) 164, 166, 334
Cfr10I RCCGGY 2 cut(s) 190, 362
Cfr13I GGNCC 5 cut(s) 188, 351, 356, 365, 1035
CpoI CGGWCCG 1 cut(s) 365
CseI GACGC 1 cut(s) 1058
Csp6I GTAC 1 cut(s) 30
CspAI ACCGGT 1 cut(s) 362
CspI CGGWCCG 1 cut(s) 365
CviAII CATG 4 cut(s) 415, 593, 904, 1210
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 6 cut(s) 536, 927, 979, 1116, 1122, 1177
DpnI GATC 6 cut(s) 407, 468, 501, 534, 574, 666
DpnII GATC 6 cut(s) 405, 466, 499, 532, 572, 664
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 378
EagI CGGCCG 1 cut(s) 378
Eam1104I CTCTTC 3 cut(s) 122, 633, 696
EarI CTCTTC 3 cut(s) 122, 633, 696
EciI GGCGGA 5 cut(s) 577, 796, 856, 871, 1065
Ecl136II GAGCTC 2 cut(s) 7, 375
EclXI CGGCCG 1 cut(s) 378
Eco24I GRGCYC 7 cut(s) 9, 176, 227, 377, 935, 980, 1117
Eco47I GGWCC 2 cut(s) 351, 365
Eco52I CGGCCG 1 cut(s) 378
Eco53kI GAGCTC 2 cut(s) 7, 375
Eco57I CTGAAG 3 cut(s) 657, 681, 827
EcoICRI GAGCTC 2 cut(s) 7, 375
EcoRI GAATTC 1 cut(s) 1213
EcoRII CCWGG 2 cut(s) 73, 432
EcoT38I GRGCYC 7 cut(s) 9, 176, 227, 377, 935, 980, 1117
FaeI CATG 4 cut(s) 418, 596, 907, 1213
FaiI YATR 6 cut(s) 308, 416, 594, 905, 1200, 1211
FalI AAGNNNNNCTT 2 cut(s) 1130, 1162
FaqI GGGAC 3 cut(s) 538, 565, 624
FatI CATG 4 cut(s) 414, 592, 903, 1209
FauI CCCGC 2 cut(s) 275, 341
FokI GGATG 2 cut(s) 1059, 1185
FriOI GRGCYC 7 cut(s) 9, 176, 227, 377, 935, 980, 1117
FspBI CTAG 2 cut(s) 104, 1227
GlaI GCGC 3 cut(s) 163, 165, 333
GsaI CCCAGC 2 cut(s) 94, 103
HaeIII GGCC 4 cut(s) 190, 357, 380, 1037
HapII CCGG 9 cut(s) 21, 177, 191, 218, 354, 363, 476, 823, 1038
HgaI GACGC 1 cut(s) 1058
HhaI GCGC 3 cut(s) 164, 166, 334
Hin1I GRCGYC 1 cut(s) 1069
Hin1II CATG 4 cut(s) 418, 596, 907, 1213
Hin6I GCGC 3 cut(s) 162, 164, 332
HinP1I GCGC 3 cut(s) 162, 164, 332
HinfI GANTC 3 cut(s) 296, 625, 1237
HpaII CCGG 9 cut(s) 21, 177, 191, 218, 354, 363, 476, 823, 1038
HphI GGTGA 1 cut(s) 812
Hpy188I TCNGA 8 cut(s) 369, 410, 537, 669, 807, 921, 982, 1172
Hpy188III TCNNGA 6 cut(s) 394, 424, 470, 629, 1060, 1088
Hpy99I CGWCG 9 cut(s) 172, 386, 623, 760, 763, 772, 775, 871, 1084
HpyAV CCTTC 4 cut(s) 750, 802, 830, 991
HpyCH4III ACNGT 2 cut(s) 212, 1055
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 5 cut(s) 232, 651, 957, 1096, 1106
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 288, 354, 1075, 1090, 1112, 1156, 1165
HpyF3I CTNAG 6 cut(s) 536, 927, 979, 1116, 1122, 1177
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 1 cut(s) 1069
Hsp92II CATG 4 cut(s) 418, 596, 907, 1213
HspAI GCGC 3 cut(s) 162, 164, 332
KpnI GGTACC 1 cut(s) 33
Kzo9I GATC 6 cut(s) 405, 466, 499, 532, 572, 664
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 7 cut(s) 4, 149, 220, 460, 843, 1008, 1202
Lsp1109I GCAGC 7 cut(s) 303, 732, 735, 858, 969, 1080, 1192
LweI GCATC 1 cut(s) 1163
MaeI CTAG 2 cut(s) 104, 1227
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 545
MalI GATC 6 cut(s) 407, 468, 501, 534, 574, 666
MbiI CCGCTC 2 cut(s) 348, 1011
MboI GATC 6 cut(s) 405, 466, 499, 532, 572, 664
MflI RGATCY 5 cut(s) 405, 466, 532, 572, 664
MhlI GDGCHC 8 cut(s) 9, 176, 217, 227, 377, 935, 980, 1117
MluCI AATT 2 cut(s) 992, 1213
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 433
MnlI CCTC 8 cut(s) 518, 531, 558, 697, 706, 976, 1117, 1125
MslI CAYNNNNRTG 1 cut(s) 604
MspI CCGG 9 cut(s) 21, 177, 191, 218, 354, 363, 476, 823, 1038
MspR9I CCNGG 4 cut(s) 21, 75, 177, 434
Mva1269I GAATGC 1 cut(s) 928
MvaI CCWGG 2 cut(s) 75, 434
MvnI CGCG 4 cut(s) 164, 334, 763, 887
MwoI GCNNNNNNNGC 8 cut(s) 100, 288, 354, 1075, 1090, 1112, 1156, 1165
NciI CCSGG 2 cut(s) 21, 177
NdeII GATC 6 cut(s) 405, 466, 499, 532, 572, 664
NlaIII CATG 4 cut(s) 418, 596, 907, 1213
NlaIV GGNNCC 5 cut(s) 31, 352, 456, 542, 1036
NmeAIII GCCGAG 1 cut(s) 356
NmuCI GTSAC 1 cut(s) 545
NspV TTCGAA 1 cut(s) 513
PaqCI CACCTGC 1 cut(s) 149
PauI GCGCGC 1 cut(s) 162
PciSI GCTCTTC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 3 cut(s) 764, 767, 872
PctI GAATGC 1 cut(s) 928
PfeI GAWTC 2 cut(s) 625, 1237
PflMI CCANNNNNTGG 1 cut(s) 451
PinAI ACCGGT 1 cut(s) 362
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp124BI GAGCTC 2 cut(s) 9, 377
Psp6I CCWGG 2 cut(s) 73, 432
PspFI CCCAGC 2 cut(s) 90, 99
PspGI CCWGG 2 cut(s) 73, 432
PspN4I GGNNCC 5 cut(s) 31, 352, 456, 542, 1036
PspPI GGNCC 5 cut(s) 188, 351, 356, 365, 1035
PstNI CAGNNNCTG 1 cut(s) 141
PsuI RGATCY 5 cut(s) 405, 466, 532, 572, 664
PteI GCGCGC 1 cut(s) 162
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 604
Rsr2I CGGWCCG 1 cut(s) 365
RsrII CGGWCCG 1 cut(s) 365
SacI GAGCTC 2 cut(s) 9, 377
SapI GCTCTTC 1 cut(s) 122
Sau3AI GATC 6 cut(s) 405, 466, 499, 532, 572, 664
Sau96I GGNCC 5 cut(s) 188, 351, 356, 365, 1035
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 4 cut(s) 21, 75, 177, 434
SduI GDGCHC 8 cut(s) 9, 176, 217, 227, 377, 935, 980, 1117
SfaNI GCATC 1 cut(s) 1163
SfcI CTRYAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 2 cut(s) 351, 365
SmiMI CAYNNNNRTG 1 cut(s) 604
Sse9I AATT 2 cut(s) 992, 1213
SspMI CTAG 2 cut(s) 104, 1227
SstI GAGCTC 2 cut(s) 9, 377
StyD4I CCNGG 4 cut(s) 19, 73, 175, 432
TaaI ACNGT 2 cut(s) 212, 1055
TaiI ACGT 1 cut(s) 159
TasI AATT 2 cut(s) 992, 1213
TauI GCSGC 4 cut(s) 562, 680, 887, 1069
TfiI GAWTC 2 cut(s) 625, 1237
TscAI CASTG 3 cut(s) 202, 217, 1108
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 7 cut(s) 291, 720, 723, 846, 957, 1093, 1205
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 3 cut(s) 431, 920, 1226
TspGWI ACGGA 3 cut(s) 196, 447, 533
TspRI CASTG 3 cut(s) 202, 217, 1108
Van91I CCANNNNNTGG 1 cut(s) 451
VpaK11BI GGWCC 2 cut(s) 351, 365
XapI RAATTY 1 cut(s) 1213
XspI CTAG 2 cut(s) 104, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.