Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006103

Physical Location & Seq

Genomic Coordinates Reverse (-)

29340048 .. 29343943

Gene Structure 3896 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1208500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1194 bp

ATGGAAGACGAAGACGGAGGGCAAAATACAGAGTCGGACCTGAACAAAAACAAGAAGAAAACAGTGATAAAAATACCGTCTTTTCAAGAAGTACTCGAGAGCTCGCAGTCTAAATCCACCCCACCTTCTCTCTTCACTCCTTCACATAGCTTTTCTCAAGCATTTGCATTCGTCAAGTCCTCTGAGTTCTACTCGCCGCCTCCTCCCTCCACCGCCGCCTCACAACCGCCGCAGGCCTCAGACGCCACCAATTCGAGGCAAACTGGCCAATCTGATGTTCCGTCTTCATCATCTTCACCTGCGAATGCAGTTGCATCATCTTCATCTGTTCAGCGTCGCAATGCAATTCTTGTGAGCCACAGACAGAAGGGAAACCCATTGCTGAAACATATTAGAAATGTGAGGTGGGAGTTTGCAGATATTGTTTGTGATTACTTACTTGGGCAAAGCTCATGCGCTCTCTATCTCAGTCTTCGGTATCATCTGCTGCATCCAGACTACCTATATTTCCGTATCAGAGAATTGCAAAAGAACTTTAAGCTTCGTGTTGTCCTTTGCCATGTTGATGTGGAAGATGTAATCAAGCCTTTACTTGAAGTTACTAAAACTGCTCTGCTTCATGAATGCGCATTATTATGCGGTTGGAGCTTGGAGGAATGTGGTCGATACTTGGAGACTATAAAAGTCTATGAAAACAAGCCTGCAGACATTATTCAAGGTCAAATGGATACAGACTATTTATCGCGGCTAAATCACGCATTAACGACAGTTCGACATGTTAACAAGACTGATGTTGTCACCCTTGGTACTACGTTCGGGTCTCTTTCTCATATCATGGATGTTTCCATGGAAGATCTAGCTCGTTGCCCTGGGATAGGAGAGCGTAAGGTGAAACGTTTGTATGATACATTTCATGAACCATTTAAGCGTGTGGTTGCCAGTCGCCCTGCTGTTCCAGAAACCTCTGTCCCGAGCAATGCTGAACCTCGTTCAGTGGATGAAGATAAAGCAGCAGAGGAAACAGAAGATGAAAGCAAACGTGGAAAGAAAGAACGAAAATTGAGTGTTAAGTCAGCCTTGTCTGCTGCCTTTTCCAAATATGCTGATAAAGTTAATAGAAAGGGCATCAACTCGCAAGGAGAGAAACAAGGAGAAAAAAGTGCTGCCATGGAAGCTGAAACTGAAAACGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000014 GO:0000109 GO:0000110 GO:0000710 GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003697 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006294 GO:0006296 GO:0006298 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010212 GO:0010213 GO:0010224 GO:0010332 GO:0016043 GO:0016787 GO:0016788 GO:0016888 GO:0016893 GO:0017108 GO:0022402 GO:0022414 GO:0022607 GO:0032991 GO:0033554 GO:0033683 GO:0034622 GO:0034641 GO:0034644 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048256 GO:0050896 GO:0051321 GO:0051716 GO:0061982 GO:0065003 GO:0065004 GO:0070522 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0104004 GO:0140097 GO:1901360 GO:1901363 GO:1903046 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03420 ko03460 map01524 map03420 map03460

Pfam Domains

Protein Families

HHH_2 HHH_5 Rad10

Protein Analysis

398

Amino Acids

44.21

Weight (kDa)

6.85

Isoelectric Point (pI)

56.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad10	PF03834	116 - 228	3.7e-44	Binding domain of DNA repair protein Ercc1 (rad10/Swi10)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05210 AT3G05210
fragaria_vesca	FvH4_6g05460 FvH4_6g05460 FvH4_6g05460 FvH4_6g05460 FvH4_6g05460 FvH4_6g05460 FvH4_6g05460
malus_domestica	MD04G1208500.v1.1
prunus_persica	Prupe.6G329400_v2.0.a1 Prupe.6G329400_v2.0.a1 Prupe.6G329400_v2.0.a1 Prupe.6G329400_v2.0.a1
pyrus_communis	pycom04g18460
rosa_chinensis	RchiOBHm_Chr3g0454231
rosa_laevigata	RLG00000025474
rosa_multiflora	Rmu_sc0026184.1_g000002 Rmu_sc0038077.1_g000001
rosa_roxburghii	Rroxscaffold_165G00437010 Rroxscaffold_6G00425350
rosa_rugosa	Rorug02G0653600
rosa_samantha	Rh3AG056900 Rh3BG058300 Rh3CG057500 Rh3DG058800
rosa_wichuraiana	Rw3G004380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	307
Acc16I	TGCGCA	1 cut(s)	628
Acc36I	ACCTGC	1 cut(s)	307
AccII	CGCG	1 cut(s)	745
AciI	CCGC	7 cut(s)	197, 213, 216, 227, 230, 639, 745
AclI	AACGTT	1 cut(s)	895
AcoI	YGGCCR	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	243
AfaI	GTAC	2 cut(s)	93, 808
AfiI	CCNNNNNNNGG	2 cut(s)	255, 875
AflIII	ACRYGT	1 cut(s)	775
AgsI	TTSAA	3 cut(s)	86, 596, 716
AjnI	CCWGG	1 cut(s)	868
AjuI	GAANNNNNNNTTGG	2 cut(s)	261, 293
AluBI	AGCT	7 cut(s)	102, 150, 450, 541, 648, 860, 1175
AluI	AGCT	7 cut(s)	102, 150, 450, 541, 648, 860, 1175
Alw21I	GWGCWC	1 cut(s)	104
Alw26I	GTCTC	2 cut(s)	668, 825
Ama87I	CYCGRG	2 cut(s)	95, 970
AoxI	GGCC	2 cut(s)	234, 265
ApeKI	GCWGC	4 cut(s)	487, 1010, 1085, 1163
AspLEI	GCGC	2 cut(s)	458, 629
AspS9I	GGNCC	1 cut(s)	37
AsuHPI	GGTGA	3 cut(s)	288, 790, 901
AvaI	CYCGRG	2 cut(s)	95, 970
AvaII	GGWCC	1 cut(s)	37
BalI	TGGCCA	1 cut(s)	267
BanII	GRGCYC	1 cut(s)	104
BbsI	GAAGAC	4 cut(s)	12, 18, 276, 464
Bbv12I	GWGCWC	1 cut(s)	104
BbvI	GCAGC	4 cut(s)	474, 1022, 1072, 1150
BciT130I	CCWGG	1 cut(s)	870
BciVI	GTATCC	1 cut(s)	721
BcoDI	GTCTC	2 cut(s)	668, 825
BfaI	CTAG	1 cut(s)	857
BfmI	CTRYAG	1 cut(s)	702
BfuAI	ACCTGC	1 cut(s)	307
BfuI	GTATCC	1 cut(s)	721
BglII	AGATCT	1 cut(s)	853
BisI	GCNGC	8 cut(s)	197, 216, 230, 488, 746, 1011, 1086, 1164
BlsI	GCNGC	8 cut(s)	198, 217, 231, 489, 747, 1012, 1087, 1165
BmcAI	AGTACT	1 cut(s)	93
Bme1390I	CCNGG	1 cut(s)	870
Bme18I	GGWCC	1 cut(s)	37
BmeT110I	CYCGRG	2 cut(s)	95, 970
BmgT120I	GGNCC	1 cut(s)	37
BmrFI	CCNGG	1 cut(s)	870
BmsI	GCATC	3 cut(s)	323, 499, 1134
BpiI	GAAGAC	4 cut(s)	12, 18, 276, 464
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpuEI	CTTGAG	1 cut(s)	141
BsaHI	GRCGYC	1 cut(s)	243
BsaI	GGTCTC	1 cut(s)	825
BsaJI	CCNNGG	5 cut(s)	802, 846, 868, 869, 1167
Bsc4I	CCNNNNNNNGG	2 cut(s)	255, 875
Bse1I	ACTGG	2 cut(s)	268, 939
Bse3DI	GCAATG	3 cut(s)	346, 377, 982
BseBI	CCWGG	1 cut(s)	870
BseDI	CCNNGG	5 cut(s)	802, 846, 868, 869, 1167
BseGI	GGATG	3 cut(s)	490, 844, 1003
BseLI	CCNNNNNNNGG	2 cut(s)	255, 875
BseMI	GCAATG	3 cut(s)	346, 377, 982
BseMII	CTCAG	3 cut(s)	174, 252, 481
BseNI	ACTGG	2 cut(s)	268, 939
BseRI	GAGGAG	1 cut(s)	192
BseXI	GCAGC	4 cut(s)	474, 1022, 1072, 1150
Bsh1236I	CGCG	1 cut(s)	745
BshFI	GGCC	2 cut(s)	236, 267
BsiHKAI	GWGCWC	1 cut(s)	104
BsiHKCI	CYCGRG	2 cut(s)	95, 970
BslFI	GGGAC	1 cut(s)	953
BslI	CCNNNNNNNGG	2 cut(s)	255, 875
BsmAI	GTCTC	2 cut(s)	668, 825
BsmFI	GGGAC	1 cut(s)	953
BsmI	GAATGC	3 cut(s)	167, 310, 629
BsnI	GGCC	2 cut(s)	236, 267
Bso31I	GGTCTC	1 cut(s)	825
BsoBI	CYCGRG	2 cut(s)	95, 970
Bsp1286I	GDGCHC	1 cut(s)	104
Bsp143I	GATC	1 cut(s)	853
Bsp19I	CCATGG	2 cut(s)	846, 1167
BspACI	CCGC	7 cut(s)	197, 213, 216, 227, 230, 639, 745
BspANI	GGCC	2 cut(s)	236, 267
BspCNI	CTCAG	3 cut(s)	175, 251, 480
BspFNI	CGCG	1 cut(s)	745
BspHI	TCATGA	2 cut(s)	619, 913
BspMAI	CTGCAG	1 cut(s)	706
BspMI	ACCTGC	1 cut(s)	307
BspTNI	GGTCTC	1 cut(s)	825
BsrDI	GCAATG	3 cut(s)	346, 377, 982
BsrI	ACTGG	2 cut(s)	268, 939
BssECI	CCNNGG	5 cut(s)	802, 846, 868, 869, 1167
BssMI	GATC	1 cut(s)	853
BssNI	GRCGYC	1 cut(s)	243
BssT1I	CCWWGG	3 cut(s)	802, 846, 1167
Bst2UI	CCWGG	1 cut(s)	870
Bst4CI	ACNGT	3 cut(s)	64, 78, 769
Bst6I	CTCTTC	1 cut(s)	137
BstACI	GRCGYC	1 cut(s)	243
BstC8I	GCNNGC	3 cut(s)	104, 234, 702
BstDEI	CTNAG	3 cut(s)	183, 238, 467
BstDSI	CCRYGG	2 cut(s)	846, 1167
BstENI	CCTNNNNNAGG	1 cut(s)	873
BstF5I	GGATG	3 cut(s)	490, 844, 1003
BstFNI	CGCG	1 cut(s)	745
BstHHI	GCGC	2 cut(s)	458, 629
BstKTI	GATC	1 cut(s)	856
BstMAI	GTCTC	2 cut(s)	668, 825
BstMBI	GATC	1 cut(s)	853
BstMWI	GCNNNNNNNGC	4 cut(s)	242, 645, 1082, 1172
BstNI	CCWGG	1 cut(s)	870
BstNSI	RCATGY	1 cut(s)	779
BstSCI	CCNGG	1 cut(s)	868
BstSFI	CTRYAG	1 cut(s)	702
BstUI	CGCG	1 cut(s)	745
BstV1I	GCAGC	4 cut(s)	474, 1022, 1072, 1150
BstV2I	GAAGAC	4 cut(s)	12, 18, 276, 464
BstX2I	RGATCY	1 cut(s)	853
BstYI	RGATCY	1 cut(s)	853
BsuI	GTATCC	1 cut(s)	721
BsuRI	GGCC	2 cut(s)	236, 267
BtgI	CCRYGG	2 cut(s)	846, 1167
BtsCI	GGATG	3 cut(s)	490, 844, 1003
BtsIMutI	CAGTG	2 cut(s)	69, 999
BveI	ACCTGC	1 cut(s)	307
Cac8I	GCNNGC	3 cut(s)	104, 234, 702
CciI	TCATGA	2 cut(s)	619, 913
CfoI	GCGC	2 cut(s)	458, 629
Cfr13I	GGNCC	1 cut(s)	37
CseI	GACGC	2 cut(s)	251, 323
Csp6I	GTAC	2 cut(s)	92, 807
CviAII	CATG	8 cut(s)	453, 560, 620, 776, 835, 847, 914, 1168
CviQI	GTAC	2 cut(s)	92, 807
DdeI	CTNAG	3 cut(s)	183, 238, 467
DpnI	GATC	1 cut(s)	855
DpnII	GATC	1 cut(s)	853
EaeI	YGGCCR	1 cut(s)	265
Eam1104I	CTCTTC	1 cut(s)	137
EarI	CTCTTC	1 cut(s)	137
Ecl136II	GAGCTC	1 cut(s)	102
Eco130I	CCWWGG	3 cut(s)	802, 846, 1167
Eco147I	AGGCCT	1 cut(s)	236
Eco24I	GRGCYC	1 cut(s)	104
Eco31I	GGTCTC	1 cut(s)	825
Eco47I	GGWCC	1 cut(s)	37
Eco53kI	GAGCTC	1 cut(s)	102
Eco88I	CYCGRG	2 cut(s)	95, 970
EcoICRI	GAGCTC	1 cut(s)	102
EcoNI	CCTNNNNNAGG	1 cut(s)	873
EcoRII	CCWGG	1 cut(s)	868
EcoT14I	CCWWGG	3 cut(s)	802, 846, 1167
EcoT38I	GRGCYC	1 cut(s)	104
ErhI	CCWWGG	3 cut(s)	802, 846, 1167
FaeI	CATG	8 cut(s)	456, 563, 623, 779, 838, 850, 917, 1171
FalI	AAGNNNNNCTT	2 cut(s)	1061, 1093
FaqI	GGGAC	1 cut(s)	953
FatI	CATG	8 cut(s)	452, 559, 619, 775, 834, 846, 913, 1167
Fnu4HI	GCNGC	8 cut(s)	197, 216, 230, 488, 746, 1011, 1086, 1164
FokI	GGATG	3 cut(s)	477, 851, 1010
FriOI	GRGCYC	1 cut(s)	104
Fsp4HI	GCNGC	8 cut(s)	197, 216, 230, 488, 746, 1011, 1086, 1164
FspAI	RTGCGCAY	1 cut(s)	628
FspBI	CTAG	1 cut(s)	857
FspI	TGCGCA	1 cut(s)	628
GlaI	GCGC	2 cut(s)	457, 628
GluI	GCNGC	8 cut(s)	197, 216, 230, 488, 746, 1011, 1086, 1164
HaeIII	GGCC	2 cut(s)	236, 267
HgaI	GACGC	2 cut(s)	251, 323
HhaI	GCGC	2 cut(s)	458, 629
Hin1I	GRCGYC	1 cut(s)	243
Hin1II	CATG	8 cut(s)	456, 563, 623, 779, 838, 850, 917, 1171
Hin6I	GCGC	2 cut(s)	456, 627
HinP1I	GCGC	2 cut(s)	456, 627
HincII	GTYRAC	1 cut(s)	781
HindII	GTYRAC	1 cut(s)	781
HindIII	AAGCTT	1 cut(s)	539
HinfI	GANTC	1 cut(s)	32
HpaI	GTTAAC	1 cut(s)	781
HphI	GGTGA	3 cut(s)	288, 790, 901
Hpy166II	GTNNAC	1 cut(s)	781
Hpy188I	TCNGA	5 cut(s)	37, 184, 241, 274, 518
Hpy188III	TCNNGA	7 cut(s)	86, 97, 494, 620, 914, 956, 970
Hpy8I	GTNNAC	1 cut(s)	781
Hpy99I	CGWCG	1 cut(s)	339
HpyAV	CCTTC	3 cut(s)	135, 150, 361
HpyCH4III	ACNGT	3 cut(s)	64, 78, 769
HpyCH4IV	ACGT	3 cut(s)	812, 895, 1039
HpyCH4V	TGCA	8 cut(s)	167, 308, 314, 344, 416, 490, 526, 704
HpyF10VI	GCNNNNNNNGC	4 cut(s)	242, 645, 1082, 1172
HpyF3I	CTNAG	3 cut(s)	183, 238, 467
HpySE526I	ACGT	3 cut(s)	812, 895, 1039
Hsp92I	GRCGYC	1 cut(s)	243
Hsp92II	CATG	8 cut(s)	456, 563, 623, 779, 838, 850, 917, 1171
HspAI	GCGC	2 cut(s)	456, 627
KspAI	GTTAAC	1 cut(s)	781
Kzo9I	GATC	1 cut(s)	853
LmnI	GCTCC	1 cut(s)	645
Lsp1109I	GCAGC	4 cut(s)	474, 1022, 1072, 1150
LweI	GCATC	3 cut(s)	323, 499, 1134
MaeI	CTAG	1 cut(s)	857
MaeII	ACGT	3 cut(s)	812, 895, 1039
MaeIII	GTNAC	2 cut(s)	598, 796
MalI	GATC	1 cut(s)	855
MboI	GATC	1 cut(s)	853
MflI	RGATCY	1 cut(s)	853
MhlI	GDGCHC	1 cut(s)	104
MlsI	TGGCCA	1 cut(s)	267
MluCI	AATT	4 cut(s)	250, 345, 521, 1058
MluNI	TGGCCA	1 cut(s)	267
MlyI	GAGTC	1 cut(s)	41
MmeI	TCCRAC	2 cut(s)	15, 623
Mox20I	TGGCCA	1 cut(s)	267
MscI	TGGCCA	1 cut(s)	267
MseI	TTAA	6 cut(s)	537, 761, 780, 924, 1068, 1113
MslI	CAYNNNNRTG	2 cut(s)	564, 634
Msp20I	TGGCCA	1 cut(s)	267
MspR9I	CCNGG	1 cut(s)	870
Mva1269I	GAATGC	3 cut(s)	167, 310, 629
MvaI	CCWGG	1 cut(s)	870
MvnI	CGCG	1 cut(s)	745
MwoI	GCNNNNNNNGC	4 cut(s)	242, 645, 1082, 1172
NcoI	CCATGG	2 cut(s)	846, 1167
NdeII	GATC	1 cut(s)	853
NlaIII	CATG	8 cut(s)	456, 563, 623, 779, 838, 850, 917, 1171
NmuCI	GTSAC	1 cut(s)	796
NsbI	TGCGCA	1 cut(s)	628
NspI	RCATGY	1 cut(s)	779
PaeR7I	CTCGAG	1 cut(s)	95
PagI	TCATGA	2 cut(s)	619, 913
PaqCI	CACCTGC	1 cut(s)	307
PasI	CCCWGGG	1 cut(s)	869
PceI	AGGCCT	1 cut(s)	236
PciI	ACATGT	1 cut(s)	775
PctI	GAATGC	3 cut(s)	167, 310, 629
PkrI	GCNGC	8 cut(s)	198, 217, 231, 489, 747, 1012, 1087, 1165
PleI	GAGTC	1 cut(s)	40
PpsI	GAGTC	1 cut(s)	40
PscI	ACATGT	1 cut(s)	775
Psp124BI	GAGCTC	1 cut(s)	104
Psp1406I	AACGTT	1 cut(s)	895
Psp6I	CCWGG	1 cut(s)	868
PspGI	CCWGG	1 cut(s)	868
PspPI	GGNCC	1 cut(s)	37
PstI	CTGCAG	1 cut(s)	706
PsuI	RGATCY	1 cut(s)	853
RsaI	GTAC	2 cut(s)	93, 808
RsaNI	GTAC	2 cut(s)	92, 807
RseI	CAYNNNNRTG	2 cut(s)	564, 634
SacI	GAGCTC	1 cut(s)	104
SaqAI	TTAA	6 cut(s)	537, 761, 780, 924, 1068, 1113
SatI	GCNGC	8 cut(s)	197, 216, 230, 488, 746, 1011, 1086, 1164
Sau3AI	GATC	1 cut(s)	853
Sau96I	GGNCC	1 cut(s)	37
ScaI	AGTACT	1 cut(s)	93
SchI	GAGTC	1 cut(s)	41
ScrFI	CCNGG	1 cut(s)	870
SduI	GDGCHC	1 cut(s)	104
SfaNI	GCATC	3 cut(s)	323, 499, 1134
SfcI	CTRYAG	1 cut(s)	702
Sfr274I	CTCGAG	1 cut(s)	95
SinI	GGWCC	1 cut(s)	37
SlaI	CTCGAG	1 cut(s)	95
SmiMI	CAYNNNNRTG	2 cut(s)	564, 634
SmlI	CTYRAG	2 cut(s)	95, 156
SmoI	CTYRAG	2 cut(s)	95, 156
Sse9I	AATT	4 cut(s)	250, 345, 521, 1058
SseBI	AGGCCT	1 cut(s)	236
SsiI	CCGC	7 cut(s)	197, 213, 216, 227, 230, 639, 745
SspMI	CTAG	1 cut(s)	857
SstI	GAGCTC	1 cut(s)	104
StuI	AGGCCT	1 cut(s)	236
StyD4I	CCNGG	1 cut(s)	868
StyI	CCWWGG	3 cut(s)	802, 846, 1167
TaaI	ACNGT	3 cut(s)	64, 78, 769
TaiI	ACGT	3 cut(s)	815, 898, 1042
TaqI	TCGA	4 cut(s)	96, 254, 664, 772
TasI	AATT	4 cut(s)	250, 345, 521, 1058
TatI	WGTACW	1 cut(s)	91
TauI	GCSGC	4 cut(s)	199, 218, 232, 748
Tru1I	TTAA	6 cut(s)	537, 761, 780, 924, 1068, 1113
Tru9I	TTAA	6 cut(s)	537, 761, 780, 924, 1068, 1113
TscAI	CASTG	2 cut(s)	69, 999
TseFI	GTSAC	1 cut(s)	796
TseI	GCWGC	4 cut(s)	487, 1010, 1085, 1163
Tsp45I	GTSAC	1 cut(s)	796
TspDTI	ATGAA	9 cut(s)	276, 312, 608, 636, 705, 902, 930, 1014, 1044
TspGWI	ACGGA	3 cut(s)	30, 270, 500
TspRI	CASTG	2 cut(s)	69, 999
VpaK11BI	GGWCC	1 cut(s)	37
XagI	CCTNNNNNAGG	1 cut(s)	873
XceI	RCATGY	1 cut(s)	779
XhoI	CTCGAG	1 cut(s)	95
XspI	CTAG	1 cut(s)	857
ZrmI	AGTACT	1 cut(s)	93

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.