MD04G1213400.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004048
Physical Location & Seq
Reverse (-)
29709771 .. 29715246
5476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1213400.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGAACTAGATGATAACTATATGGAAACAGATAACAGGAAGGAAATAGAGAGTCCTGCAGAAGCGTTGAATAATCCAGAGTCATGTGATCAAATCCTCTCTCCTGCAGAAACATCCAACCCACTGAAGTCATCTGAACTGAAGATCCCCTCTTCTGCTGAAGCGTTTTGTGGACCTGCTGAAGCGTTGAATAATCCAGAGTCATCTGAACAAATCCACTCTCCTGTGGAAACATCCAACACACCAGAGTCATCTGAACTGAAGATCCCCTCTTCTGCTCAAGTGATCAGTTTACCTGAAGTGTTGGATAATCCAGAGCCATCAGAACAAATCCCCTCACCTGCAGAAACCTCCAACACCCCATATTCATCTGAACCAAAGATCTTTTCTTCTGCTGAAGTGTTCGGTGTACCTGAGTCTGTTGAAAAGAAAATGTCTTCTCCTGAGAAAGTGTCTAAGAAACCTGATTTATCTGAAAAGAAAACTGCCTCTTCTGCTGGAGCATCCAGTTCTGGAAAGAGGATCCCAAGATCATTGAAGGGAAAAGCTAAAATTGTGAAGAAAACTCTTGTTCAAAACAGTTTGAAAACTAGCAAAGGTACAGGTACTTCACAAGTTAATGGGAGGAAAAGGAAAAGGAATAGGGGTAACAACGAGAGTAGCAAAACAAGAGAAGAAGATGGCAAGAAGCTGAGTTTAAGTGAACAGAACCAACAGAATATCTTGAAAGAAGAGGCTACAGAGAAGAGCCATCAGGCTCAGAAGAATCCAGAAAAATTTGACGAATCCGAAAAGAGCCAACAAAAACGGAGTACAAAAAAACGACGTGAGTCAGACAAGAGTGTCAAGAGTGAAAAAAACAGTGAAAAACGTGATTATAAGAGAGAAAAGCTTGGTGGTTTGATCTTCATGTGCAGCGGAAAGACAAAGCCAGACTGTTTCCATTACCGTATCATGGGTGTTACACTGGGTAAAAAAGATGTCGTTTTAGGTATCAAACCTGGGTTGAAGCTTTTTCTCTATGATTTCGATCTTAAGCTTTTATATGGGGTTTACAAAGCATCCTCTCGTGGAGGCATAAAACTTGAGCCAAAAGCTTTTGGTGGGGCCTTCCCTGCTCAGGTGCGGTTCAATGTGGAGAAAGATTGTCTTCCCCTACCTGAGAGTGTTTTCAAGAGGGCAATAAAGGAAAACTATGATGGGAAAAACAAGTTCAAAACTGAACTTACAGTTCGACAAGTCAGAAGGCTCACAGCACTTTTCCGACCAGCTCAAGTGCATTCATCAGTTCTAGCCCCCCGTTCCCCGCTGGAAACAAAAGCTCGGGATCGGGGAGTTCATGAGGGGGTGAGAGAATCACGGCCGCTTTCACACAGGGATGCACGTACTAGAGATCCTTATGCCCATGCTGATGCAAGGAGGTACCCGGTGTTAGCTCATGAAAGGGACCAGCATGTTGCACACCGAGAGGTGGTACCTGCAAGGAGAGAGGAAACTTCTCATGATTTGTACCCAACTGAAAAGGAATATCGAGCTTATGGTCTTCAGGGAGACAGGAGAAATGTGACTTCTCTTCCAATTACTTCAGCTGTGGAATCTCGCCATAGGGACTATGAAGGAGAGCATCTTCTAAGACAGACAAACTTCATTTACAGAGATCCTGTTCCTGCACATCGAGAGAATGTTAATTCCGATCATCTCTACTTGAATGATCCTCTTACTCTTGGTGCTAGACATGAGTTGCCACCTGCAACATCTGCTACTGCTGTCGATGCATATGCAAGGGAGCCATATTATCGTTCCTATCATGGTTCTTCATTGTCGGACCCATACCTGGCACCCTATAGGAGAGATGATGTTCTGTCAGGATCTTATTCCGTTGGTGGAAGGAGAGAGAACTACCTAATTGAGGCTGATCCCGTGCAAAGGAGAGAAACCAGCTACGGACCAAGAGACAGGTACCTGATTGAGACTGATCCTGTGTATAGGAGAGAACCTGATCGTCATGTAGAGAGATTATCATTGTACTCGAGACATGATGCTGCTGCTGCTGATGCTTCATTAGGTTATAACCAGAGAGAAACCTACCAGGCGACCAACCCGGATCCTGTGCATGCACCAGTCTCATCTCGTTATGCTTTTGCAGGTCCGTCATATACCTACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

723

Amino Acids

81.35

Weight (kDa)

9.14

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 299 - 421 3.4e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 879, 2070
AarI CACCTGC 2 cut(s) 349, 1756
AasI GACNNNNNNGTC 1 cut(s) 842
Acc36I ACCTGC 5 cut(s) 184, 349, 1486, 1756, 2135
Acc65I GGTACC 3 cut(s) 1422, 1474, 1959
AccB1I GGYRCC 4 cut(s) 1422, 1474, 1837, 1959
AciI CCGC 5 cut(s) 918, 1126, 1307, 1364, 2164
AcoI YGGCCR 1 cut(s) 1361
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 9 cut(s) 146, 161, 180, 201, 281, 318, 417, 1529, 1569
AfiI CCNNNNNNNGG 6 cut(s) 955, 1120, 1471, 1834, 1909, 1926
AflII CTTAAG 1 cut(s) 1034
AjiI CACGTC 1 cut(s) 827
AjnI CCWGG 3 cut(s) 1000, 1833, 2088
AjuI GAANNNNNNNTTGG 2 cut(s) 370, 402
AloI GAACNNNNNNTCC 4 cut(s) 129, 161, 249, 281
Alw26I GTCTC 5 cut(s) 1545, 1947, 1964, 2026, 2128
Ama87I CYCGRG 2 cut(s) 1323, 2029
AoxI GGCC 2 cut(s) 1107, 1361
ApeKI GCWGC 4 cut(s) 915, 2042, 2045, 2048
ApoI RAATTY 1 cut(s) 776
Asp718I GGTACC 3 cut(s) 1422, 1474, 1959
AspS9I GGNCC 6 cut(s) 173, 1107, 1447, 1825, 1946, 2147
AsuC2I CCSGG 2 cut(s) 1427, 2102
AsuHPI GGTGA 2 cut(s) 330, 1360
AvaI CYCGRG 2 cut(s) 1323, 2029
AvaII GGWCC 5 cut(s) 173, 1447, 1825, 1946, 2147
BamHI GGATCC 2 cut(s) 522, 2104
BanI GGYRCC 4 cut(s) 1422, 1474, 1837, 1959
BarI GAAGNNNNNNTAC 2 cut(s) 592, 624
BauI CACGAG 1 cut(s) 1068
BbsI GAAGAC 3 cut(s) 429, 1142, 1535
BbvI GCAGC 4 cut(s) 927, 2029, 2032, 2035
BccI CCATC 4 cut(s) 328, 674, 759, 1193
BceAI ACGGC 1 cut(s) 1376
BcgI CGANNNNNNTGC 2 cut(s) 1760, 1794
BciT130I CCWGG 3 cut(s) 1002, 1835, 2090
BclI TGATCA 2 cut(s) 88, 285
BcnI CCSGG 2 cut(s) 1427, 2102
BcoDI GTCTC 5 cut(s) 1545, 1947, 1964, 2026, 2128
BfaI CTAG 5 cut(s) 8, 591, 1292, 1389, 1731
BfmI CTRYAG 5 cut(s) 57, 105, 342, 738, 1843
BfrI CTTAAG 1 cut(s) 1034
BfuAI ACCTGC 5 cut(s) 184, 349, 1486, 1756, 2135
BglII AGATCT 1 cut(s) 381
BisI GCNGC 5 cut(s) 916, 1364, 2043, 2046, 2049
BlsI GCNGC 5 cut(s) 917, 1365, 2044, 2047, 2050
Bme1390I CCNGG 5 cut(s) 1002, 1427, 1835, 2090, 2102
Bme18I GGWCC 5 cut(s) 173, 1447, 1825, 1946, 2147
BmeT110I CYCGRG 2 cut(s) 1323, 2029
BmgBI CACGTC 1 cut(s) 827
BmgT120I GGNCC 6 cut(s) 173, 1107, 1447, 1825, 1946, 2147
BmrFI CCNGG 5 cut(s) 1002, 1427, 1835, 2090, 2102
BmrI ACTGGG 1 cut(s) 977
BmsI GCATC 8 cut(s) 512, 1070, 1369, 1402, 1633, 1762, 2029, 2044
BmuI ACTGGG 1 cut(s) 977
BoxI GACNNNNGTC 1 cut(s) 829
BpiI GAAGAC 3 cut(s) 429, 1142, 1535
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 1119
BpuEI CTTGAG 3 cut(s) 264, 1106, 1257
BpuMI CCSGG 2 cut(s) 1427, 2102
BsaAI YACGTR 1 cut(s) 1385
BsaBI GATNNNNATC 1 cut(s) 1029
BsaJI CCNNGG 1 cut(s) 1001
Bsc4I CCNNNNNNNGG 6 cut(s) 955, 1120, 1471, 1834, 1909, 1926
Bse1I ACTGG 3 cut(s) 507, 972, 2120
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 3 cut(s) 1002, 1835, 2090
BseDI CCNNGG 1 cut(s) 1001
BseGI GGATG 5 cut(s) 113, 233, 503, 1061, 1384
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 6 cut(s) 955, 1120, 1471, 1834, 1909, 1926
BseMII CTCAG 6 cut(s) 405, 435, 683, 773, 1133, 1152
BseNI ACTGG 3 cut(s) 507, 972, 2120
BseX3I CGGCCG 1 cut(s) 1361
BseXI GCAGC 4 cut(s) 927, 2029, 2032, 2035
BsgI GTGCAG 2 cut(s) 934, 1653
Bsh1285I CGRYCG 1 cut(s) 1364
BshFI GGCC 2 cut(s) 1109, 1363
BshNI GGYRCC 4 cut(s) 1422, 1474, 1837, 1959
BsiEI CGRYCG 1 cut(s) 1364
BsiHKCI CYCGRG 2 cut(s) 1323, 2029
BsiSI CCGG 2 cut(s) 1427, 2102
BslFI GGGAC 2 cut(s) 1460, 1622
BslI CCNNNNNNNGG 6 cut(s) 955, 1120, 1471, 1834, 1909, 1926
BsmAI GTCTC 5 cut(s) 1545, 1947, 1964, 2026, 2128
BsmFI GGGAC 2 cut(s) 1460, 1622
BsmI GAATGC 1 cut(s) 1279
BsnI GGCC 2 cut(s) 1109, 1363
BsoBI CYCGRG 2 cut(s) 1323, 2029
BspACI CCGC 5 cut(s) 918, 1126, 1307, 1364, 2164
BspANI GGCC 2 cut(s) 1109, 1363
BspCNI CTCAG 6 cut(s) 406, 436, 684, 772, 1132, 1153
BspHI TCATGA 3 cut(s) 1339, 1438, 1501
BspMAI CTGCAG 3 cut(s) 61, 109, 346
BspMI ACCTGC 5 cut(s) 184, 349, 1486, 1756, 2135
BspQI GCTCTTC 1 cut(s) 740
BspT107I GGYRCC 4 cut(s) 1422, 1474, 1837, 1959
BspTI CTTAAG 1 cut(s) 1034
BsrI ACTGG 3 cut(s) 507, 972, 2120
BssECI CCNNGG 1 cut(s) 1001
BssSI CACGAG 1 cut(s) 1068
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 3 cut(s) 1002, 1835, 2090
Bst4CI ACNGT 5 cut(s) 581, 863, 938, 950, 1231
Bst6I CTCTTC 6 cut(s) 157, 277, 496, 726, 740, 1578
BstAFI CTTAAG 1 cut(s) 1034
BstAPI GCANNNNNTGC 1 cut(s) 1757
BstBAI YACGTR 1 cut(s) 1385
BstC8I GCNNGC 1 cut(s) 2115
BstDEI CTNAG 8 cut(s) 414, 444, 456, 692, 759, 1119, 1161, 1631
BstENI CCTNNNNNAGG 1 cut(s) 1907
BstF5I GGATG 5 cut(s) 113, 233, 503, 1061, 1384
BstMAI GTCTC 5 cut(s) 1545, 1947, 1964, 2026, 2128
BstMCI CGRYCG 1 cut(s) 1364
BstMWI GCNNNNNNNGC 6 cut(s) 494, 1115, 1757, 1772, 2048, 2054
BstNI CCWGG 3 cut(s) 1002, 1835, 2090
BstNSI RCATGY 2 cut(s) 1457, 2117
BstPAI GACNNNNGTC 1 cut(s) 829
BstSCI CCNGG 5 cut(s) 1000, 1425, 1833, 2088, 2100
BstSFI CTRYAG 5 cut(s) 57, 105, 342, 738, 1843
BstV1I GCAGC 4 cut(s) 927, 2029, 2032, 2035
BstV2I GAAGAC 3 cut(s) 429, 1142, 1535
BstX2I RGATCY 8 cut(s) 144, 264, 381, 522, 1393, 1657, 1868, 2104
BstYI RGATCY 8 cut(s) 144, 264, 381, 522, 1393, 1657, 1868, 2104
BstZI CGGCCG 1 cut(s) 1361
BsuRI GGCC 2 cut(s) 1109, 1363
BtrI CACGTC 1 cut(s) 827
BtsCI GGATG 5 cut(s) 113, 233, 503, 1061, 1384
BtsIMutI CAGTG 3 cut(s) 122, 868, 965
BveI ACCTGC 5 cut(s) 184, 349, 1486, 1756, 2135
Cac8I GCNNGC 1 cut(s) 2115
CciI TCATGA 3 cut(s) 1339, 1438, 1501
Cfr13I GGNCC 6 cut(s) 173, 1107, 1447, 1825, 1946, 2147
DdeI CTNAG 8 cut(s) 414, 444, 456, 692, 759, 1119, 1161, 1631
DrdI GACNNNNNNGTC 1 cut(s) 842
DseDI GACNNNNNNGTC 1 cut(s) 842
EaeI YGGCCR 1 cut(s) 1361
EagI CGGCCG 1 cut(s) 1361
Eam1104I CTCTTC 6 cut(s) 157, 277, 496, 726, 740, 1578
EarI CTCTTC 6 cut(s) 157, 277, 496, 726, 740, 1578
EclXI CGGCCG 1 cut(s) 1361
Eco47I GGWCC 5 cut(s) 173, 1447, 1825, 1946, 2147
Eco52I CGGCCG 1 cut(s) 1361
Eco57I CTGAAG 9 cut(s) 146, 161, 180, 201, 281, 318, 417, 1529, 1569
Eco88I CYCGRG 2 cut(s) 1323, 2029
EcoNI CCTNNNNNAGG 1 cut(s) 1907
EcoO109I RGGNCCY 1 cut(s) 1107
EcoRII CCWGG 3 cut(s) 1000, 1833, 2088
EcoT22I ATGCAT 1 cut(s) 1777
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FaqI GGGAC 2 cut(s) 1460, 1622
FauI CCCGC 1 cut(s) 1314
FauNDI CATATG 1 cut(s) 1777
FbaI TGATCA 2 cut(s) 88, 285
Fnu4HI GCNGC 5 cut(s) 916, 1364, 2043, 2046, 2049
FokI GGATG 5 cut(s) 100, 220, 490, 1048, 1391
Fsp4HI GCNGC 5 cut(s) 916, 1364, 2043, 2046, 2049
FspBI CTAG 5 cut(s) 8, 591, 1292, 1389, 1731
GluI GCNGC 5 cut(s) 916, 1364, 2043, 2046, 2049
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 2 cut(s) 1109, 1363
HapII CCGG 2 cut(s) 1427, 2102
HindIII AAGCTT 4 cut(s) 890, 1010, 1037, 1095
HpaII CCGG 2 cut(s) 1427, 2102
HphI GGTGA 2 cut(s) 330, 1360
Hpy166II GTNNAC 5 cut(s) 173, 293, 410, 704, 1054
Hpy8I GTNNAC 5 cut(s) 173, 293, 410, 704, 1054
Hpy99I CGWCG 1 cut(s) 828
HpyAV CCTTC 6 cut(s) 34, 532, 1120, 1239, 1610, 1881
HpyCH4III ACNGT 5 cut(s) 581, 863, 938, 950, 1231
HpyCH4IV ACGT 3 cut(s) 826, 871, 1384
HpyF10VI GCNNNNNNNGC 6 cut(s) 494, 1115, 1757, 1772, 2048, 2054
HpyF3I CTNAG 8 cut(s) 414, 444, 456, 692, 759, 1119, 1161, 1631
HpySE526I ACGT 3 cut(s) 826, 871, 1384
KpnI GGTACC 3 cut(s) 1426, 1478, 1963
Ksp22I TGATCA 2 cut(s) 88, 285
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 2 cut(s) 500, 1786
Lsp1109I GCAGC 4 cut(s) 927, 2029, 2032, 2035
LweI GCATC 8 cut(s) 512, 1070, 1369, 1402, 1633, 1762, 2029, 2044
MaeI CTAG 5 cut(s) 8, 591, 1292, 1389, 1731
MaeII ACGT 3 cut(s) 826, 871, 1384
MaeIII GTNAC 3 cut(s) 647, 961, 1564
MflI RGATCY 8 cut(s) 144, 264, 381, 522, 1393, 1657, 1868, 2104
MluCI AATT 5 cut(s) 552, 776, 1578, 1687, 1905
MlyI GAGTC 6 cut(s) 61, 89, 209, 257, 425, 839
MmeI TCCRAC 6 cut(s) 141, 261, 285, 378, 1288, 1803
Mph1103I ATGCAT 1 cut(s) 1777
MseI TTAA 4 cut(s) 618, 698, 1035, 1686
MslI CAYNNNNRTG 2 cut(s) 1377, 1410
MspA1I CMGCKG 3 cut(s) 918, 1309, 1589
MspCI CTTAAG 1 cut(s) 1034
MspI CCGG 2 cut(s) 1427, 2102
MspR9I CCNGG 5 cut(s) 1002, 1427, 1835, 2090, 2102
Mva1269I GAATGC 1 cut(s) 1279
MvaI CCWGG 3 cut(s) 1002, 1835, 2090
MwoI GCNNNNNNNGC 6 cut(s) 494, 1115, 1757, 1772, 2048, 2054
NciI CCSGG 2 cut(s) 1427, 2102
NdeI CATATG 1 cut(s) 1777
NmuCI GTSAC 1 cut(s) 1564
NsiI ATGCAT 1 cut(s) 1777
NspI RCATGY 2 cut(s) 1457, 2117
PaeI GCATGC 1 cut(s) 2117
PaeR7I CTCGAG 1 cut(s) 2029
PagI TCATGA 3 cut(s) 1339, 1438, 1501
PaqCI CACCTGC 2 cut(s) 349, 1756
PciSI GCTCTTC 1 cut(s) 740
PctI GAATGC 1 cut(s) 1279
PfeI GAWTC 4 cut(s) 766, 785, 1355, 1595
PkrI GCNGC 5 cut(s) 917, 1365, 2044, 2047, 2050
PleI GAGTC 6 cut(s) 60, 88, 208, 256, 424, 838
PpsI GAGTC 6 cut(s) 60, 88, 208, 256, 424, 838
Ppu21I YACGTR 1 cut(s) 1385
PshAI GACNNNNGTC 1 cut(s) 829
PsiI TTATAA 2 cut(s) 879, 2070
Psp6I CCWGG 3 cut(s) 1000, 1833, 2088
PspGI CCWGG 3 cut(s) 1000, 1833, 2088
PspPI GGNCC 6 cut(s) 173, 1107, 1447, 1825, 1946, 2147
PstI CTGCAG 3 cut(s) 61, 109, 346
PsuI RGATCY 8 cut(s) 144, 264, 381, 522, 1393, 1657, 1868, 2104
PvuII CAGCTG 1 cut(s) 1589
RseI CAYNNNNRTG 2 cut(s) 1377, 1410
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 4 cut(s) 618, 698, 1035, 1686
SatI GCNGC 5 cut(s) 916, 1364, 2043, 2046, 2049
Sau96I GGNCC 6 cut(s) 173, 1107, 1447, 1825, 1946, 2147
SchI GAGTC 6 cut(s) 61, 89, 209, 257, 425, 839
ScrFI CCNGG 5 cut(s) 1002, 1427, 1835, 2090, 2102
SfaNI GCATC 8 cut(s) 512, 1070, 1369, 1402, 1633, 1762, 2029, 2044
SfcI CTRYAG 5 cut(s) 57, 105, 342, 738, 1843
Sfr274I CTCGAG 1 cut(s) 2029
SinI GGWCC 5 cut(s) 173, 1447, 1825, 1946, 2147
SlaI CTCGAG 1 cut(s) 2029
SmiMI CAYNNNNRTG 2 cut(s) 1377, 1410
SmlI CTYRAG 5 cut(s) 279, 1034, 1085, 1272, 2029
SmoI CTYRAG 5 cut(s) 279, 1034, 1085, 1272, 2029
SphI GCATGC 1 cut(s) 2117
Sse9I AATT 5 cut(s) 552, 776, 1578, 1687, 1905
SsiI CCGC 5 cut(s) 918, 1126, 1307, 1364, 2164
SspMI CTAG 5 cut(s) 8, 591, 1292, 1389, 1731
StyD4I CCNGG 5 cut(s) 1000, 1425, 1833, 2088, 2100
TaaI ACNGT 5 cut(s) 581, 863, 938, 950, 1231
TaiI ACGT 3 cut(s) 829, 874, 1387
TaqI TCGA 6 cut(s) 1029, 1234, 1531, 1675, 1770, 2030
TasI AATT 5 cut(s) 552, 776, 1578, 1687, 1905
TatI WGTACW 2 cut(s) 812, 2025
TauI GCSGC 1 cut(s) 1366
TfiI GAWTC 4 cut(s) 766, 785, 1355, 1595
Tru1I TTAA 4 cut(s) 618, 698, 1035, 1686
Tru9I TTAA 4 cut(s) 618, 698, 1035, 1686
TscAI CASTG 3 cut(s) 129, 868, 972
TseFI GTSAC 1 cut(s) 1564
TseI GCWGC 4 cut(s) 915, 2042, 2045, 2048
Tsp45I GTSAC 1 cut(s) 1564
TspDTI ATGAA 9 cut(s) 357, 898, 1272, 1328, 1455, 1629, 1636, 1806, 2049
TspGWI ACGGA 4 cut(s) 823, 1867, 1959, 2139
TspRI CASTG 3 cut(s) 129, 868, 972
Vha464I CTTAAG 1 cut(s) 1034
VpaK11BI GGWCC 5 cut(s) 173, 1447, 1825, 1946, 2147
XagI CCTNNNNNAGG 1 cut(s) 1907
XapI RAATTY 1 cut(s) 776
XceI RCATGY 2 cut(s) 1457, 2117
XhoI CTCGAG 1 cut(s) 2029
XspI CTAG 5 cut(s) 8, 591, 1292, 1389, 1731
Zsp2I ATGCAT 1 cut(s) 1777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.