MD12G1228500.v1.1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004048
Physical Location & Seq
Reverse (-)
30401068 .. 30406431
5364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1228500.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGAACTAGATGATAACTATATGGAAACAGATAACAGGAGGGAAATAGAGAGTCCTGCAGAAGTGTTTAATAATCCAGAGCCATCTGAGCAAATCATCTCTCCGGCAGAAACAACCAGCCCACTGGAGTCATCTGAACTGAAGGTCCCCTCTTCTGCTGAAGTGATCAGTGTACCTGCTGAATTGTTGAATAATCCAGAGCCATCAGAACAAGTCCCCTCACCTGCAGAAACCTCCAACACCCAAGAGTCATCTGAACTAAAGTTCCCTTCTTCTGCTGAAGTGTTTGGTGGACCTGCTGAAGTGTTGAATAATCCAGTGTCATCTGAACAAATCCCCTCTCCTGCAGAAACATCCAGCACACCAGAGTCATCTGAACTGAAGGCCCCCTCGTCTTCTGAAGCGTTCAGTGGACCTGAGTCAGCTGAAAAGAAAATGGCTTCTCCTGGGGAAGGGTCTAAGAAACCTGATTTATCTGAAAAGAAAACTTCTTCTGCGGGAGCATCCATTTCTGGAAAGAGGATCCCAAGATCATTGAAGGGAAAAGCTAAAATCGTGAAGAAAACTCCTATTCAAAACAGTTTGAAAATTAGTAGAGGTGCAGATACTTCACAGGTTAATGGGAGGAAAAGGAAAAGGAATAGGGGTAACAACGTGAGTAGAAAAACAAGAGAACAAGATGGCAAAAAGCTGAGTTCAACGAAAGAGAACCAACAGAATATCTCGAACGAAGAGGCTACAGAGAAGAGCCATCAGGTTCAAAAGAATCCAGGAAAATCTGATGAATCCGAAAAGAGCCAACAAAAACAGATGAGTACAGAAAAACGACGTGAGTCAGACAAGAGTGCCAAGAGTGAAAAGAACAGTGAAAAACGTGATGATAAGAGAGAAAAGCTTGGCGGTTTGATCTTCATGTGCAGTGGAAAGACAAGGCCAGACTGTTTCCAATACCGTATCATGGGTGTTACAATGGGTAAAAAAGAAGTTGTTTTAGGTATCAAACCTGGGTTGAAGCTTTTTCTCTATGATTTCGATCGTAAGGTTTTGTACGGGGTTTACAAAGCATCCTCTTCTGGAGGCTTAAAACTTGAGCCAAAGGCTTTTGGTGGGGCCTTCCCTGCTCAGGTGCGGTTCAATGTGGAGAAAGATTGTCTCCCTCTGCCTGAAAGTGTTTTCAAGAAGGCAATAAAGGAAAACTATGATAAGAAAAACAAGTTCAAAACTGAACTTACAGTCCGACAAGTCAGGAGGCTCACAGCACTTTTCCGACCAGCTCAAGTGCATTCATCGGTTTTAGCCCCCCGTTCCTCGCCGGAAGCAAAAGCTCGGGATCGGGGAGTTCATGAGGGGGTGAGAGAATCACGGCCACTTTCACAAAGGGATGCACATACTATAGATCCTTATGCCCATACTGATGCTAGGAGTTATCCGGTGTTTGCCCATGAAAGGGACCGGCGTGTTGCATACCGAGAAGTGGCACCTGCAAGAAGAGAGGAGACTTCTCATGATTTGTACATAACTGAAAAGGAATATCGAGCTTATGGTCTTCGGGGAGACAGGGGAAATGTGACTTCTCATTCAATTACTTCTGGGGAATCGCGCCGTAGGGAGTATGAAGGAGAGCATCTTCTACGACAGACAAACTTCATTTACAGAGATGATGTCCCTACACATCGAGAGAATGTTCATTCTGATCGTCCTTACTTGAATGACACTCATACTCTTGGCACCAGACATGAATTGCCACCTGCAACATCTGCTACTGCCGTGGATGCATATGCAAGGGATCCATATTATCGCTCCTATTATGATTCTTCATTGTCGGACCCATACCTGGCTCCCAATAGGAGAGAGGATGTTATGTCAGGGCCTTATTCCGTTGGTGGAAGGAGGGAGAACTACCTAATTGAGGCTGATCCTGTGCAAAGGAGAGAAACCAGCAACAGACGAAGAGACAGTTACCGGATTGAGACTGATCCTGTGTATAGGAGAGAACCCGTTCGTCATGTAGAGAGATTGTCATTGTACTCGAGGCAAGATGCTGCTGCTGCTGATGCTCCATTAGGTTATAACCTTACAGAAGCCTACCAGGTGACCAAGCCGGATCCTGTGCATGCACCAGTTTCCTCTCGTTATGCTTTTGCAGGTCCGTCATACACCTTCCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

723

Amino Acids

81.1

Weight (kDa)

9.18

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 300 - 422 4e-49 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2070
AarI CACCTGC 3 cut(s) 232, 1489, 1756
Acc36I ACCTGC 6 cut(s) 184, 232, 304, 1489, 1756, 2135
AccB1I GGYRCC 2 cut(s) 1477, 1727
AccII CGCG 1 cut(s) 1600
AciI CCGC 4 cut(s) 497, 900, 1129, 2164
AcoI YGGCCR 1 cut(s) 1364
AcuI CTGAAG 6 cut(s) 161, 180, 300, 321, 401, 420
AfaI GTAC 5 cut(s) 174, 817, 1049, 1514, 2027
AfiI CCNNNNNNNGG 8 cut(s) 452, 958, 1123, 1446, 1447, 1474, 1834, 1909
AjiI CACGTC 1 cut(s) 830
AjnI CCWGG 5 cut(s) 445, 769, 1003, 1833, 2088
AloI GAACNNNNNNTCC 6 cut(s) 129, 161, 249, 281, 1218, 1250
AluBI AGCT 8 cut(s) 425, 548, 691, 895, 1015, 1274, 1325, 1538
AluI AGCT 8 cut(s) 425, 548, 691, 895, 1015, 1274, 1325, 1538
Alw26I GTCTC 5 cut(s) 1157, 1490, 1548, 1947, 1964
Ama87I CYCGRG 2 cut(s) 1326, 2029
AoxI GGCC 5 cut(s) 384, 932, 1110, 1364, 1868
ApeKI GCWGC 3 cut(s) 2042, 2045, 2048
Asp700I GAANNNNTTC 1 cut(s) 1998
AspLEI GCGC 1 cut(s) 1602
AspS9I GGNCC 9 cut(s) 145, 293, 385, 413, 1110, 1450, 1825, 1868, 2147
AsuHPI GGTGA 3 cut(s) 213, 1363, 2104
AvaI CYCGRG 2 cut(s) 1326, 2029
AvaII GGWCC 6 cut(s) 145, 293, 413, 1450, 1825, 2147
BamHI GGATCC 3 cut(s) 522, 1786, 2104
BanI GGYRCC 2 cut(s) 1477, 1727
BbsI GAAGAC 2 cut(s) 387, 1538
BbvI GCAGC 3 cut(s) 2029, 2032, 2035
BccI CCATC 4 cut(s) 91, 211, 674, 759
BceAI ACGGC 3 cut(s) 1379, 1587, 1751
BciT130I CCWGG 5 cut(s) 447, 771, 1005, 1835, 2090
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 5 cut(s) 1157, 1490, 1548, 1947, 1964
BfaI CTAG 2 cut(s) 8, 1419
BfmI CTRYAG 5 cut(s) 57, 225, 345, 738, 1392
BfuAI ACCTGC 6 cut(s) 184, 232, 304, 1489, 1756, 2135
BisI GCNGC 3 cut(s) 2043, 2046, 2049
BlsI GCNGC 3 cut(s) 2044, 2047, 2050
Bme1390I CCNGG 5 cut(s) 447, 771, 1005, 1835, 2090
Bme18I GGWCC 6 cut(s) 145, 293, 413, 1450, 1825, 2147
BmeT110I CYCGRG 2 cut(s) 1326, 2029
BmgBI CACGTC 1 cut(s) 830
BmgT120I GGNCC 9 cut(s) 145, 293, 385, 413, 1110, 1450, 1825, 1868, 2147
BmrFI CCNGG 5 cut(s) 447, 771, 1005, 1835, 2090
BmsI GCATC 8 cut(s) 512, 1073, 1372, 1405, 1633, 1762, 2029, 2044
BoxI GACNNNNGTC 2 cut(s) 418, 832
BpiI GAAGAC 2 cut(s) 387, 1538
BpmI CTGGAG 2 cut(s) 146, 1095
Bpu10I CCTNAGC 1 cut(s) 1122
BpuEI CTTGAG 2 cut(s) 1109, 1260
BsaBI GATNNNNATC 1 cut(s) 1032
BsaJI CCNNGG 3 cut(s) 446, 1004, 1767
BsaWI WCCGGW 2 cut(s) 1429, 1962
Bsc4I CCNNNNNNNGG 8 cut(s) 452, 958, 1123, 1446, 1447, 1474, 1834, 1909
Bse118I RCCGGY 1 cut(s) 1452
Bse1I ACTGG 3 cut(s) 129, 317, 2120
Bse8I GATNNNNATC 1 cut(s) 1032
BseBI CCWGG 5 cut(s) 447, 771, 1005, 1835, 2090
BseDI CCNNGG 3 cut(s) 446, 1004, 1767
BseGI GGATG 6 cut(s) 353, 503, 1064, 1387, 1777, 1861
BseJI GATNNNNATC 1 cut(s) 1032
BseLI CCNNNNNNNGG 8 cut(s) 452, 958, 1123, 1446, 1447, 1474, 1834, 1909
BseMII CTCAG 4 cut(s) 78, 408, 683, 1136
BseNI ACTGG 3 cut(s) 129, 317, 2120
BseRI GAGGAG 1 cut(s) 1508
BseXI GCAGC 3 cut(s) 2029, 2032, 2035
BsgI GTGCAG 2 cut(s) 621, 937
Bsh1236I CGCG 1 cut(s) 1600
Bsh1285I CGRYCG 1 cut(s) 1036
BshFI GGCC 5 cut(s) 386, 934, 1112, 1366, 1870
BshNI GGYRCC 2 cut(s) 1477, 1727
BsiEI CGRYCG 1 cut(s) 1036
BsiHKCI CYCGRG 2 cut(s) 1326, 2029
BsiSI CCGG 6 cut(s) 104, 1313, 1430, 1453, 1963, 2102
BslFI GGGAC 4 cut(s) 131, 200, 1463, 1649
BslI CCNNNNNNNGG 8 cut(s) 452, 958, 1123, 1446, 1447, 1474, 1834, 1909
BsmAI GTCTC 5 cut(s) 1157, 1490, 1548, 1947, 1964
BsmFI GGGAC 4 cut(s) 131, 200, 1463, 1649
BsmI GAATGC 1 cut(s) 1282
BsnI GGCC 5 cut(s) 386, 934, 1112, 1366, 1870
BsoBI CYCGRG 2 cut(s) 1326, 2029
Bsp1407I TGTACA 1 cut(s) 1512
BspACI CCGC 4 cut(s) 497, 900, 1129, 2164
BspANI GGCC 5 cut(s) 386, 934, 1112, 1366, 1870
BspCNI CTCAG 4 cut(s) 79, 409, 684, 1135
BspFNI CGCG 1 cut(s) 1600
BspHI TCATGA 2 cut(s) 1342, 1504
BspMAI CTGCAG 3 cut(s) 61, 229, 349
BspMI ACCTGC 6 cut(s) 184, 232, 304, 1489, 1756, 2135
BspQI GCTCTTC 1 cut(s) 740
BspT107I GGYRCC 2 cut(s) 1477, 1727
BsrFI RCCGGY 1 cut(s) 1452
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 3 cut(s) 129, 317, 2120
BssAI RCCGGY 1 cut(s) 1452
BssECI CCNNGG 3 cut(s) 446, 1004, 1767
Bst2UI CCWGG 5 cut(s) 447, 771, 1005, 1835, 2090
Bst4CI ACNGT 6 cut(s) 581, 866, 941, 953, 1234, 1958
Bst6I CTCTTC 6 cut(s) 157, 726, 740, 1075, 1483, 1945
BstAPI GCANNNNNTGC 1 cut(s) 1757
BstAUI TGTACA 1 cut(s) 1512
BstC8I GCNNGC 1 cut(s) 2115
BstDEI CTNAG 5 cut(s) 87, 417, 459, 692, 1122
BstDSI CCRYGG 1 cut(s) 1767
BstEII GGTNACC 1 cut(s) 2092
BstENI CCTNNNNNAGG 2 cut(s) 450, 1907
BstF5I GGATG 6 cut(s) 353, 503, 1064, 1387, 1777, 1861
BstFNI CGCG 1 cut(s) 1600
BstHHI GCGC 1 cut(s) 1602
BstMAI GTCTC 5 cut(s) 1157, 1490, 1548, 1947, 1964
BstMCI CGRYCG 1 cut(s) 1036
BstMWI GCNNNNNNNGC 6 cut(s) 88, 1118, 1757, 1772, 2048, 2054
BstNI CCWGG 5 cut(s) 447, 771, 1005, 1835, 2090
BstNSI RCATGY 1 cut(s) 2117
BstPAI GACNNNNGTC 2 cut(s) 418, 832
BstPI GGTNACC 1 cut(s) 2092
BstSCI CCNGG 5 cut(s) 445, 769, 1003, 1833, 2088
BstSFI CTRYAG 5 cut(s) 57, 225, 345, 738, 1392
BstUI CGCG 1 cut(s) 1600
BstV1I GCAGC 3 cut(s) 2029, 2032, 2035
BstV2I GAAGAC 2 cut(s) 387, 1538
BstX2I RGATCY 4 cut(s) 522, 1396, 1786, 2104
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 4 cut(s) 522, 1396, 1786, 2104
BsuRI GGCC 5 cut(s) 386, 934, 1112, 1366, 1870
BtgI CCRYGG 1 cut(s) 1767
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 6 cut(s) 353, 503, 1064, 1387, 1777, 1861
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 6 cut(s) 122, 175, 324, 415, 871, 925
BveI ACCTGC 6 cut(s) 184, 232, 304, 1489, 1756, 2135
Cac8I GCNNGC 1 cut(s) 2115
CciI TCATGA 2 cut(s) 1342, 1504
CfoI GCGC 1 cut(s) 1602
Cfr10I RCCGGY 1 cut(s) 1452
Cfr13I GGNCC 9 cut(s) 145, 293, 385, 413, 1110, 1450, 1825, 1868, 2147
CsiI ACCWGGT 1 cut(s) 2088
Csp6I GTAC 5 cut(s) 173, 816, 1048, 1513, 2026
CviAII CATG 8 cut(s) 913, 958, 1343, 1442, 1505, 1736, 2006, 2114
CviQI GTAC 5 cut(s) 173, 816, 1048, 1513, 2026
DdeI CTNAG 5 cut(s) 87, 417, 459, 692, 1122
EaeI YGGCCR 1 cut(s) 1364
Eam1104I CTCTTC 6 cut(s) 157, 726, 740, 1075, 1483, 1945
EarI CTCTTC 6 cut(s) 157, 726, 740, 1075, 1483, 1945
Eco47I GGWCC 6 cut(s) 145, 293, 413, 1450, 1825, 2147
Eco57I CTGAAG 6 cut(s) 161, 180, 300, 321, 401, 420
Eco88I CYCGRG 2 cut(s) 1326, 2029
Eco91I GGTNACC 1 cut(s) 2092
EcoNI CCTNNNNNAGG 2 cut(s) 450, 1907
EcoO109I RGGNCCY 4 cut(s) 145, 385, 1110, 1868
EcoO65I GGTNACC 1 cut(s) 2092
EcoRII CCWGG 5 cut(s) 445, 769, 1003, 1833, 2088
EcoT22I ATGCAT 1 cut(s) 1777
FaeI CATG 8 cut(s) 916, 961, 1346, 1445, 1508, 1739, 2009, 2117
FaqI GGGAC 4 cut(s) 131, 200, 1463, 1649
FatI CATG 8 cut(s) 912, 957, 1342, 1441, 1504, 1735, 2005, 2113
FauI CCCGC 1 cut(s) 490
FauNDI CATATG 1 cut(s) 1777
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 2043, 2046, 2049
FokI GGATG 6 cut(s) 340, 490, 1051, 1394, 1784, 1868
Fsp4HI GCNGC 3 cut(s) 2043, 2046, 2049
FspBI CTAG 2 cut(s) 8, 1419
GlaI GCGC 1 cut(s) 1601
GluI GCNGC 3 cut(s) 2043, 2046, 2049
GsuI CTGGAG 2 cut(s) 146, 1095
HaeIII GGCC 5 cut(s) 386, 934, 1112, 1366, 1870
HapII CCGG 6 cut(s) 104, 1313, 1430, 1453, 1963, 2102
HhaI GCGC 1 cut(s) 1602
Hin1II CATG 8 cut(s) 916, 961, 1346, 1445, 1508, 1739, 2009, 2117
Hin6I GCGC 1 cut(s) 1600
HinP1I GCGC 1 cut(s) 1600
HindIII AAGCTT 2 cut(s) 893, 1013
HpaII CCGG 6 cut(s) 104, 1313, 1430, 1453, 1963, 2102
HphI GGTGA 3 cut(s) 213, 1363, 2104
Hpy166II GTNNAC 4 cut(s) 173, 293, 413, 1057
Hpy8I GTNNAC 4 cut(s) 173, 293, 413, 1057
Hpy99I CGWCG 1 cut(s) 831
HpyAV CCTTC 9 cut(s) 136, 279, 376, 446, 532, 1123, 1174, 1610, 1881
HpyCH4III ACNGT 6 cut(s) 581, 866, 941, 953, 1234, 1958
HpyCH4IV ACGT 3 cut(s) 654, 829, 874
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 1118, 1757, 1772, 2048, 2054
HpyF3I CTNAG 5 cut(s) 87, 417, 459, 692, 1122
HpySE526I ACGT 3 cut(s) 654, 829, 874
Hsp92II CATG 8 cut(s) 916, 961, 1346, 1445, 1508, 1739, 2009, 2117
HspAI GCGC 1 cut(s) 1600
Ksp22I TGATCA 1 cut(s) 165
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 4 cut(s) 500, 1805, 1843, 2062
Lsp1109I GCAGC 3 cut(s) 2029, 2032, 2035
LweI GCATC 8 cut(s) 512, 1073, 1372, 1405, 1633, 1762, 2029, 2044
MabI ACCWGGT 1 cut(s) 2088
MaeI CTAG 2 cut(s) 8, 1419
MaeII ACGT 3 cut(s) 654, 829, 874
MaeIII GTNAC 5 cut(s) 647, 964, 1567, 1958, 2092
MflI RGATCY 4 cut(s) 522, 1396, 1786, 2104
MluCI AATT 5 cut(s) 182, 588, 1581, 1739, 1905
MlyI GAGTC 6 cut(s) 61, 137, 257, 377, 428, 842
MmeI TCCRAC 4 cut(s) 261, 1261, 1291, 1803
Mph1103I ATGCAT 1 cut(s) 1777
MroXI GAANNNNTTC 1 cut(s) 1998
MseI TTAA 3 cut(s) 69, 618, 1082
MslI CAYNNNNRTG 1 cut(s) 1413
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 6 cut(s) 104, 1313, 1430, 1453, 1963, 2102
MspR9I CCNGG 5 cut(s) 447, 771, 1005, 1835, 2090
Mva1269I GAATGC 1 cut(s) 1282
MvaI CCWGG 5 cut(s) 447, 771, 1005, 1835, 2090
MvnI CGCG 1 cut(s) 1600
MwoI GCNNNNNNNGC 6 cut(s) 88, 1118, 1757, 1772, 2048, 2054
NdeI CATATG 1 cut(s) 1777
NlaIII CATG 8 cut(s) 916, 961, 1346, 1445, 1508, 1739, 2009, 2117
NmuCI GTSAC 2 cut(s) 1567, 2092
NsiI ATGCAT 1 cut(s) 1777
NspI RCATGY 1 cut(s) 2117
PaeI GCATGC 1 cut(s) 2117
PaeR7I CTCGAG 1 cut(s) 2029
PagI TCATGA 2 cut(s) 1342, 1504
PaqCI CACCTGC 3 cut(s) 232, 1489, 1756
PciSI GCTCTTC 1 cut(s) 740
PctI GAATGC 1 cut(s) 1282
PdmI GAANNNNTTC 1 cut(s) 1998
PfeI GAWTC 5 cut(s) 766, 785, 1358, 1595, 1811
PfoI TCCNGGA 1 cut(s) 769
PkrI GCNGC 3 cut(s) 2044, 2047, 2050
Ple19I CGATCG 1 cut(s) 1036
PleI GAGTC 6 cut(s) 60, 136, 256, 376, 427, 841
PpsI GAGTC 6 cut(s) 60, 136, 256, 376, 427, 841
PpuMI RGGWCCY 1 cut(s) 145
PshAI GACNNNNGTC 2 cut(s) 418, 832
PsiI TTATAA 1 cut(s) 2070
Psp5II RGGWCCY 1 cut(s) 145
Psp6I CCWGG 5 cut(s) 445, 769, 1003, 1833, 2088
PspEI GGTNACC 1 cut(s) 2092
PspGI CCWGG 5 cut(s) 445, 769, 1003, 1833, 2088
PspPI GGNCC 9 cut(s) 145, 293, 385, 413, 1110, 1450, 1825, 1868, 2147
PspPPI RGGWCCY 1 cut(s) 145
PspXI VCTCGAGB 1 cut(s) 2029
PstI CTGCAG 3 cut(s) 61, 229, 349
PsuI RGATCY 4 cut(s) 522, 1396, 1786, 2104
PvuI CGATCG 1 cut(s) 1036
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 5 cut(s) 174, 817, 1049, 1514, 2027
RsaNI GTAC 5 cut(s) 173, 816, 1048, 1513, 2026
RseI CAYNNNNRTG 1 cut(s) 1413
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 3 cut(s) 69, 618, 1082
SatI GCNGC 3 cut(s) 2043, 2046, 2049
Sau96I GGNCC 9 cut(s) 145, 293, 385, 413, 1110, 1450, 1825, 1868, 2147
SchI GAGTC 6 cut(s) 61, 137, 257, 377, 428, 842
ScrFI CCNGG 5 cut(s) 447, 771, 1005, 1835, 2090
SexAI ACCWGGT 1 cut(s) 2088
SfaNI GCATC 8 cut(s) 512, 1073, 1372, 1405, 1633, 1762, 2029, 2044
SfcI CTRYAG 5 cut(s) 57, 225, 345, 738, 1392
Sfr274I CTCGAG 1 cut(s) 2029
SinI GGWCC 6 cut(s) 145, 293, 413, 1450, 1825, 2147
SlaI CTCGAG 1 cut(s) 2029
SmiMI CAYNNNNRTG 1 cut(s) 1413
SmlI CTYRAG 3 cut(s) 1088, 1275, 2029
SmoI CTYRAG 3 cut(s) 1088, 1275, 2029
SphI GCATGC 1 cut(s) 2117
Sse9I AATT 5 cut(s) 182, 588, 1581, 1739, 1905
SsiI CCGC 4 cut(s) 497, 900, 1129, 2164
SspMI CTAG 2 cut(s) 8, 1419
StyD4I CCNGG 5 cut(s) 445, 769, 1003, 1833, 2088
TaaI ACNGT 6 cut(s) 581, 866, 941, 953, 1234, 1958
TaiI ACGT 3 cut(s) 657, 832, 877
TaqI TCGA 5 cut(s) 725, 1032, 1534, 1675, 2030
TasI AATT 5 cut(s) 182, 588, 1581, 1739, 1905
TatI WGTACW 3 cut(s) 815, 1512, 2025
TfiI GAWTC 5 cut(s) 766, 785, 1358, 1595, 1811
Tru1I TTAA 3 cut(s) 69, 618, 1082
Tru9I TTAA 3 cut(s) 69, 618, 1082
TscAI CASTG 6 cut(s) 129, 175, 324, 415, 871, 925
TseFI GTSAC 2 cut(s) 1567, 2092
TseI GCWGC 3 cut(s) 2042, 2045, 2048
Tsp45I GTSAC 2 cut(s) 1567, 2092
TspGWI ACGGA 2 cut(s) 1867, 2139
TspRI CASTG 6 cut(s) 129, 175, 324, 415, 871, 925
VpaK11BI GGWCC 6 cut(s) 145, 293, 413, 1450, 1825, 2147
XagI CCTNNNNNAGG 2 cut(s) 450, 1907
XceI RCATGY 1 cut(s) 2117
XhoI CTCGAG 1 cut(s) 2029
XmnI GAANNNNTTC 1 cut(s) 1998
XspI CTAG 2 cut(s) 8, 1419
Zsp2I ATGCAT 1 cut(s) 1777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.