Prupe.6G333600_v2.0.a1

DCD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0004048
Physical Location & Seq
Reverse (-)
28998159 .. 29003939
5781 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G333600.10

Sequence Viewer

Length: 2142 bp
ATGGAACTAGATGATAACTATATGGAAACAGATAACAGGAAGGAGATAAATAGTCCTGCAGTAGTGTTGAATAATTCAGAGTCATCTGAGCAAATCCCTTCCCCTGCAGAAGCATCCAACAACCCGGAGTCATCTGAACAAATCCCCTCTCCAGCAGAAGCATCCAAAAAACCAGACTCATCTGAACTGAAGACCTCTTCTGCTGAAGCGTTCAGTGGACCTGAGTTAGCTAAAATGAAAATGTCTTCTCCTGCAGAAGTGTCTAATAAACCTGATGTATCTGAAAAGAAAATTGCCTCTCCTGCAGAAGCATCCAACTCTGGAAAGAAAACCCCAAGATCATTGAAGGGCAAAGCTAAAATAGTGAAGAAAACTCCCATTCATAACAGCTTGAAAACAAGCAAAGGTACAACTAGCTCACAGGTTAATGGGAGGAAACGAAGGCGGAATAGGGGTAACAACGAGAGTAGCAACAAAAGAGAAGAAGATGCCAAGAAGATGAATTCAAAAAGAGAGAAACAAGAGAATATCACGAATGAAAAGCATGCCGAGAAGAGCCATCGGGCTCAAAAGAATCCAGGAAAGATTGACGAATCCGAAAAGAGCCTACAAAACCAGCAGAGTACAGAAAAACGACGTGGGTCAGACAAGAGTGTGAAGAGTCAAAGGAACAGCGAAAAACATGATGAAAAGAGGGAAAAGCTTGGTGGTTTGATCTTCATGTGCAGTGGAAAGACAAAGCCAGACTGCTTCCATTACCGTATCATGGGTGTTTCAATGGGTAAAAAAGACCTTGTGTTGGGTATCAAACCTGGGTTGAAGCTTTTTCTCTTTGATTTTGATCTTAAGCTTTTGTATGGGGTTTACAAAGCATCCTCTTCAGGTGGCCTAAAACTCGAGCCAAAAGCATTTGGTGGAGCCTTCCCTGCTCAGGTGCGGTTCAACGTGGAGAAAGATTGTCTTCCTCTACCTGAGAGCGTTTTCAAGAAGGCAATACAAGAAAACTATAATGAGAAAAAGAAGTTCAAAACAGAACTTACAGTTCGACAAGTCAGGAAGCTCACAGCACTTTTCCGACCAGCTCAAGTCCATTCAACTGCTCTAGCTACCCGTTCCCCAGTCCAGGCTAAAGTTCGGGATCGTGGAGTTCATGAAGGAGCAAGGGAATCAATGCCACATGCCCACAGGGATGCACATGCTAGAGATCCTTATGCCAATGGTGATGCTAGGAGTTATCCAGTGTTAGCTCATGAACGGGACCAGCATGTTAAATACCGAGATGTGGCATCAGTAAGGAGAGAGGAAACTTCTCATGATTTATACCTTACTGAAAAGGAATATCGAGCTTATGGTCTTCGGGGAGAGAGAAGAAATGTGTCTCCTCGTATCGTTCCTCCAGCTCTGGAAGCTCGCCATAGAGAGTATGAAAGAGAGCATCTTGCAAGACAGCCAAACCTCATTTACCGGGAGGCTGTCCCTGCACATCAAGAAAATGTTCATAACGTGTCTCCTCGTATCGTTCCTCCAGCTCTGGAAGCTCGCCATAGAGAGTATGAAAGAGAGCATCTTGCAAGACAGCCAACCCTCATTTACCGGGAGGCTGTCCCTGCACATCGAGAAAATGTTCATTCCGATCCTCTCTACTTGAATGATAGAGATTACCCTGCTTATACTCACGGTGCTAGACATGAGTTGCCACCTGCAACATCTGCTACTGCTGTAGATGCGTATGCAAGGGATCCATATTACGGCTACTCCTATTATGGTTCTTCATCATTGGACCCATATTTGGCACCCCAGAGGAGAGAGGAAGTTCCTTCAGGATCTTTTTCTGTTGTCGGGAGGAGAGAGAATTATCTAATTGAAACTGATCCTCCAAGGAGAGAAACTGATCGTGTGGAGAGATTATACTCAACACATGCTTTTGGTGGGAGGAGAGAGAACTGCCTGATTGAGACTGATCCTGTGCTAAGGAGAGAAACTGATCGTGGAGAGAGACTATACTCGATGCATGCTGCTCCTGCTGATGCTGCATCCGGTTATAACAGGACAGAAAACTACCAGGCAACCAAGGCTGATGCATTGCCTGCGCCAGTTTCGTCTCGTTATGCTTTTGCAGGTCCGTCGTACTCCTACCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dev_Cell_Death

Protein Analysis

714

Amino Acids

80.64

Weight (kDa)

9.48

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2043
AarI CACCTGC 1 cut(s) 1708
Acc36I ACCTGC 2 cut(s) 1708, 2108
AccB1I GGYRCC 1 cut(s) 1792
AciI CCGC 2 cut(s) 445, 937
AclWI GGATC 8 cut(s) 1146, 1199, 1628, 1733, 1746, 1831, 1865, 1955
AcsI RAATTY 1 cut(s) 502
AcuI CTGAAG 4 cut(s) 209, 225, 864, 1803
AfaI GTAC 3 cut(s) 409, 625, 2129
AfiI CCNNNNNNNGG 7 cut(s) 766, 799, 931, 1123, 1282, 1748, 1789
AflII CTTAAG 1 cut(s) 845
AflIII ACRYGT 1 cut(s) 1503
AjiI CACGTC 1 cut(s) 638
AjnI CCWGG 4 cut(s) 577, 811, 1122, 2061
Alw26I GTCTC 5 cut(s) 1383, 1512, 1949, 1990, 2106
AlwI GGATC 8 cut(s) 1146, 1199, 1628, 1733, 1746, 1831, 1865, 1955
Ama87I CYCGRG 1 cut(s) 896
AoxI GGCC 1 cut(s) 886
ApeKI GCWGC 2 cut(s) 2015, 2030
ApoI RAATTY 1 cut(s) 502
Asp700I GAANNNNTTC 2 cut(s) 1494, 1623
AspLEI GCGC 1 cut(s) 2092
AspS9I GGNCC 4 cut(s) 218, 1258, 1780, 2120
AsuC2I CCSGG 3 cut(s) 125, 1466, 1595
AsuHPI GGTGA 1 cut(s) 1232
AvaI CYCGRG 1 cut(s) 896
AvaII GGWCC 4 cut(s) 218, 1258, 1780, 2120
BamHI GGATCC 1 cut(s) 1738
BanI GGYRCC 1 cut(s) 1792
BanII GRGCYC 1 cut(s) 568
BbsI GAAGAC 4 cut(s) 197, 237, 953, 1346
BbvI GCAGC 2 cut(s) 2002, 2017
BccI CCATC 1 cut(s) 567
BceAI ACGGC 1 cut(s) 1765
BcgI CGANNNNNNTGC 2 cut(s) 2106, 2140
BciT130I CCWGG 4 cut(s) 579, 813, 1124, 2063
BcnI CCSGG 3 cut(s) 125, 1466, 1595
BcoDI GTCTC 5 cut(s) 1383, 1512, 1949, 1990, 2106
BfaI CTAG 6 cut(s) 8, 414, 1103, 1200, 1227, 1683
BfmI CTRYAG 5 cut(s) 57, 105, 252, 303, 1719
BfrI CTTAAG 1 cut(s) 845
BfuAI ACCTGC 2 cut(s) 1708, 2108
BisI GCNGC 2 cut(s) 2016, 2031
BlsI GCNGC 2 cut(s) 2017, 2032
Bme1390I CCNGG 7 cut(s) 125, 579, 813, 1124, 1466, 1595, 2063
Bme18I GGWCC 4 cut(s) 218, 1258, 1780, 2120
BmeT110I CYCGRG 1 cut(s) 896
BmgBI CACGTC 1 cut(s) 638
BmgT120I GGNCC 4 cut(s) 218, 1258, 1780, 2120
BmiI GGNNCC 5 cut(s) 919, 1259, 1740, 1782, 1794
BmrFI CCNGG 7 cut(s) 125, 579, 813, 1124, 1466, 1595, 2063
BmrI ACTGGG 1 cut(s) 1112
BmuI ACTGGG 1 cut(s) 1112
BoxI GACNNNNGTC 1 cut(s) 640
BpiI GAAGAC 4 cut(s) 197, 237, 953, 1346
BpmI CTGGAG 3 cut(s) 135, 1380, 1509
Bpu10I CCTNAGC 2 cut(s) 930, 1970
BpuEI CTTGAG 1 cut(s) 1068
BpuMI CCSGG 3 cut(s) 125, 1466, 1595
BsaBI GATNNNNATC 1 cut(s) 840
BsaJI CCNNGG 3 cut(s) 812, 1877, 2070
BsaWI WCCGGW 1 cut(s) 2036
BsaXI ACNNNNNCTCC 4 cut(s) 1739, 1769, 1858, 1888
Bsc4I CCNNNNNNNGG 7 cut(s) 766, 799, 931, 1123, 1282, 1748, 1789
Bse1I ACTGG 3 cut(s) 1118, 1238, 2093
Bse3DI GCAATG 1 cut(s) 2081
Bse8I GATNNNNATC 1 cut(s) 840
BseBI CCWGG 4 cut(s) 579, 813, 1124, 2063
BseDI CCNNGG 3 cut(s) 812, 1877, 2070
BseGI GGATG 6 cut(s) 113, 161, 311, 872, 1195, 2033
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 7 cut(s) 766, 799, 931, 1123, 1282, 1748, 1789
BseMI GCAATG 1 cut(s) 2081
BseMII CTCAG 4 cut(s) 78, 213, 944, 963
BseNI ACTGG 3 cut(s) 1118, 1238, 2093
BseRI GAGGAG 5 cut(s) 1371, 1500, 1816, 1858, 1948
BseXI GCAGC 2 cut(s) 2002, 2017
BsgI GTGCAG 3 cut(s) 745, 1464, 1593
BshFI GGCC 1 cut(s) 888
BshNI GGYRCC 1 cut(s) 1792
BsiHKCI CYCGRG 1 cut(s) 896
BsiSI CCGG 4 cut(s) 125, 1465, 1594, 2037
BslFI GGGAC 3 cut(s) 1271, 1460, 1589
BslI CCNNNNNNNGG 7 cut(s) 766, 799, 931, 1123, 1282, 1748, 1789
BsmAI GTCTC 5 cut(s) 1383, 1512, 1949, 1990, 2106
BsmBI CGTCTC 1 cut(s) 2106
BsmFI GGGAC 3 cut(s) 1271, 1460, 1589
BsnI GGCC 1 cut(s) 888
BsoBI CYCGRG 1 cut(s) 896
Bsp1286I GDGCHC 1 cut(s) 568
BspACI CCGC 2 cut(s) 445, 937
BspANI GGCC 1 cut(s) 888
BspCNI CTCAG 4 cut(s) 79, 214, 943, 964
BspHI TCATGA 3 cut(s) 1150, 1249, 1312
BspLI GGNNCC 5 cut(s) 919, 1259, 1740, 1782, 1794
BspMAI CTGCAG 4 cut(s) 61, 109, 256, 307
BspMI ACCTGC 2 cut(s) 1708, 2108
BspPI GGATC 8 cut(s) 1146, 1199, 1628, 1733, 1746, 1831, 1865, 1955
BspQI GCTCTTC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 1792
BspTI CTTAAG 1 cut(s) 845
BsrDI GCAATG 1 cut(s) 2081
BsrI ACTGG 3 cut(s) 1118, 1238, 2093
BssECI CCNNGG 3 cut(s) 812, 1877, 2070
BssT1I CCWWGG 2 cut(s) 1877, 2070
Bst2UI CCWGG 4 cut(s) 579, 813, 1124, 2063
Bst4CI ACNGT 4 cut(s) 761, 1042, 1679, 2138
Bst6I CTCTTC 4 cut(s) 202, 548, 653, 883
BstAFI CTTAAG 1 cut(s) 845
BstAPI GCANNNNNTGC 2 cut(s) 1709, 2087
BstC8I GCNNGC 5 cut(s) 546, 1411, 1540, 2013, 2088
BstDEI CTNAG 5 cut(s) 87, 222, 930, 972, 1970
BstF5I GGATG 6 cut(s) 113, 161, 311, 872, 1195, 2033
BstHHI GCGC 1 cut(s) 2092
BstMAI GTCTC 5 cut(s) 1383, 1512, 1949, 1990, 2106
BstNI CCWGG 4 cut(s) 579, 813, 1124, 2063
BstNSI RCATGY 6 cut(s) 548, 1181, 1199, 1268, 1922, 2015
BstPAI GACNNNNGTC 1 cut(s) 640
BstSCI CCNGG 7 cut(s) 123, 577, 811, 1122, 1464, 1593, 2061
BstSFI CTRYAG 5 cut(s) 57, 105, 252, 303, 1719
BstV1I GCAGC 2 cut(s) 2002, 2017
BstV2I GAAGAC 4 cut(s) 197, 237, 953, 1346
BstX2I RGATCY 3 cut(s) 1204, 1738, 1823
BstYI RGATCY 3 cut(s) 1204, 1738, 1823
BsuRI GGCC 1 cut(s) 888
BtrI CACGTC 1 cut(s) 638
BtsCI GGATG 6 cut(s) 113, 161, 311, 872, 1195, 2033
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 3 cut(s) 220, 733, 1245
BveI ACCTGC 2 cut(s) 1708, 2108
Cac8I GCNNGC 5 cut(s) 546, 1411, 1540, 2013, 2088
CciI TCATGA 3 cut(s) 1150, 1249, 1312
CfoI GCGC 1 cut(s) 2092
Cfr13I GGNCC 4 cut(s) 218, 1258, 1780, 2120
Csp6I GTAC 3 cut(s) 408, 624, 2128
CviQI GTAC 3 cut(s) 408, 624, 2128
DdeI CTNAG 5 cut(s) 87, 222, 930, 972, 1970
Eam1104I CTCTTC 4 cut(s) 202, 548, 653, 883
EarI CTCTTC 4 cut(s) 202, 548, 653, 883
EciI GGCGGA 1 cut(s) 460
Eco130I CCWWGG 2 cut(s) 1877, 2070
Eco24I GRGCYC 1 cut(s) 568
Eco47I GGWCC 4 cut(s) 218, 1258, 1780, 2120
Eco57I CTGAAG 4 cut(s) 209, 225, 864, 1803
Eco88I CYCGRG 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 4 cut(s) 577, 811, 1122, 2061
EcoT14I CCWWGG 2 cut(s) 1877, 2070
EcoT22I ATGCAT 2 cut(s) 2013, 2083
EcoT38I GRGCYC 1 cut(s) 568
ErhI CCWWGG 2 cut(s) 1877, 2070
Esp3I CGTCTC 1 cut(s) 2106
FalI AAGNNNNNCTT 2 cut(s) 945, 977
FaqI GGGAC 3 cut(s) 1271, 1460, 1589
Fnu4HI GCNGC 2 cut(s) 2016, 2031
FokI GGATG 6 cut(s) 100, 148, 298, 859, 1202, 2020
FriOI GRGCYC 1 cut(s) 568
Fsp4HI GCNGC 2 cut(s) 2016, 2031
FspBI CTAG 6 cut(s) 8, 414, 1103, 1200, 1227, 1683
GlaI GCGC 1 cut(s) 2091
GluI GCNGC 2 cut(s) 2016, 2031
GsuI CTGGAG 3 cut(s) 135, 1380, 1509
HaeIII GGCC 1 cut(s) 888
HapII CCGG 4 cut(s) 125, 1465, 1594, 2037
HhaI GCGC 1 cut(s) 2092
Hin6I GCGC 1 cut(s) 2090
HinP1I GCGC 1 cut(s) 2090
HindIII AAGCTT 3 cut(s) 701, 821, 848
HinfI GANTC 7 cut(s) 80, 128, 176, 574, 593, 661, 1166
HpaII CCGG 4 cut(s) 125, 1465, 1594, 2037
HphI GGTGA 1 cut(s) 1232
Hpy166II GTNNAC 2 cut(s) 218, 865
Hpy188I TCNGA 9 cut(s) 79, 88, 136, 184, 283, 598, 646, 1076, 1633
Hpy8I GTNNAC 2 cut(s) 218, 865
Hpy99I CGWCG 2 cut(s) 639, 2128
HpyAV CCTTC 8 cut(s) 34, 108, 340, 435, 931, 982, 1148, 1827
HpyCH4III ACNGT 4 cut(s) 761, 1042, 1679, 2138
HpyCH4IV ACGT 3 cut(s) 637, 945, 1503
HpyF3I CTNAG 5 cut(s) 87, 222, 930, 972, 1970
HpySE526I ACGT 3 cut(s) 637, 945, 1503
HspAI GCGC 1 cut(s) 2090
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 3 cut(s) 917, 1157, 2023
Lsp1109I GCAGC 2 cut(s) 2002, 2017
MaeI CTAG 6 cut(s) 8, 414, 1103, 1200, 1227, 1683
MaeII ACGT 3 cut(s) 637, 945, 1503
MaeIII GTNAC 1 cut(s) 455
MflI RGATCY 3 cut(s) 1204, 1738, 1823
MhlI GDGCHC 1 cut(s) 568
MluCI AATT 5 cut(s) 73, 291, 502, 1852, 1860
MlyI GAGTC 4 cut(s) 89, 137, 170, 670
MmeI TCCRAC 3 cut(s) 141, 339, 1099
Mph1103I ATGCAT 2 cut(s) 2013, 2083
MroXI GAANNNNTTC 2 cut(s) 1494, 1623
MseI TTAA 4 cut(s) 426, 846, 1269, 2140
MslI CAYNNNNRTG 1 cut(s) 1188
MspCI CTTAAG 1 cut(s) 845
MspI CCGG 4 cut(s) 125, 1465, 1594, 2037
MspR9I CCNGG 7 cut(s) 125, 579, 813, 1124, 1466, 1595, 2063
MvaI CCWGG 4 cut(s) 579, 813, 1124, 2063
NciI CCSGG 3 cut(s) 125, 1466, 1595
NlaIV GGNNCC 5 cut(s) 919, 1259, 1740, 1782, 1794
NmeAIII GCCGAG 1 cut(s) 574
NsiI ATGCAT 2 cut(s) 2013, 2083
NspI RCATGY 6 cut(s) 548, 1181, 1199, 1268, 1922, 2015
PaeI GCATGC 2 cut(s) 548, 2015
PaeR7I CTCGAG 1 cut(s) 896
PagI TCATGA 3 cut(s) 1150, 1249, 1312
PaqCI CACCTGC 1 cut(s) 1708
PciSI GCTCTTC 1 cut(s) 548
PdmI GAANNNNTTC 2 cut(s) 1494, 1623
PfeI GAWTC 3 cut(s) 574, 593, 1166
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 2 cut(s) 2017, 2032
PleI GAGTC 4 cut(s) 88, 136, 170, 669
PpsI GAGTC 4 cut(s) 88, 136, 170, 669
PshAI GACNNNNGTC 1 cut(s) 640
PsiI TTATAA 1 cut(s) 2043
Psp6I CCWGG 4 cut(s) 577, 811, 1122, 2061
PspGI CCWGG 4 cut(s) 577, 811, 1122, 2061
PspN4I GGNNCC 5 cut(s) 919, 1259, 1740, 1782, 1794
PspPI GGNCC 4 cut(s) 218, 1258, 1780, 2120
PspXI VCTCGAGB 1 cut(s) 896
PstI CTGCAG 4 cut(s) 61, 109, 256, 307
PsuI RGATCY 3 cut(s) 1204, 1738, 1823
RsaI GTAC 3 cut(s) 409, 625, 2129
RsaNI GTAC 3 cut(s) 408, 624, 2128
RseI CAYNNNNRTG 1 cut(s) 1188
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 4 cut(s) 426, 846, 1269, 2140
SatI GCNGC 2 cut(s) 2016, 2031
Sau96I GGNCC 4 cut(s) 218, 1258, 1780, 2120
SchI GAGTC 4 cut(s) 89, 137, 170, 670
ScrFI CCNGG 7 cut(s) 125, 579, 813, 1124, 1466, 1595, 2063
SduI GDGCHC 1 cut(s) 568
SfcI CTRYAG 5 cut(s) 57, 105, 252, 303, 1719
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 4 cut(s) 218, 1258, 1780, 2120
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 1188
SmlI CTYRAG 3 cut(s) 845, 896, 1083
SmoI CTYRAG 3 cut(s) 845, 896, 1083
SphI GCATGC 2 cut(s) 548, 2015
Sse9I AATT 5 cut(s) 73, 291, 502, 1852, 1860
SsiI CCGC 2 cut(s) 445, 937
SspMI CTAG 6 cut(s) 8, 414, 1103, 1200, 1227, 1683
StyD4I CCNGG 7 cut(s) 123, 577, 811, 1122, 1464, 1593, 2061
StyI CCWWGG 2 cut(s) 1877, 2070
TaaI ACNGT 4 cut(s) 761, 1042, 1679, 2138
TaiI ACGT 3 cut(s) 640, 948, 1506
TaqI TCGA 5 cut(s) 897, 1045, 1342, 1615, 2006
TasI AATT 5 cut(s) 73, 291, 502, 1852, 1860
TatI WGTACW 1 cut(s) 623
TfiI GAWTC 3 cut(s) 574, 593, 1166
Tru1I TTAA 4 cut(s) 426, 846, 1269, 2140
Tru9I TTAA 4 cut(s) 426, 846, 1269, 2140
TscAI CASTG 3 cut(s) 220, 733, 1245
TseI GCWGC 2 cut(s) 2015, 2030
TspGWI ACGGA 1 cut(s) 2112
TspRI CASTG 3 cut(s) 220, 733, 1245
Vha464I CTTAAG 1 cut(s) 845
VpaK11BI GGWCC 4 cut(s) 218, 1258, 1780, 2120
XapI RAATTY 1 cut(s) 502
XceI RCATGY 6 cut(s) 548, 1181, 1199, 1268, 1922, 2015
XhoI CTCGAG 1 cut(s) 896
XmnI GAANNNNTTC 2 cut(s) 1494, 1623
XspI CTAG 6 cut(s) 8, 414, 1103, 1200, 1227, 1683
Zsp2I ATGCAT 2 cut(s) 2013, 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.