RchiOBHm_Chr3g0453261

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004048
Physical Location & Seq
Forward (+)
3708465 .. 3713961
5497 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42036

Sequence Viewer

Length: 2394 bp
ATGGATCACAATAACTATACGAAAACAGACAAGGGGAATGAAATAAAGAGTCCTACAGAAGGTTTGAATAATACAGAATCTTCTGACCATATCCCCTCCTCTCCTGCAGAAGCACCCAACAAACCAGTGTCATCTGAACAGGAGATCCCTTCTTCTGTGGAACTGCTAAGTACAACTGAGTTAGAACCATTGAATAATCCAGAGTCATCAGGTTATATCTCCTCTCCTGCAGAAGCACCCAACAAACCGGAGTCATCTGAACAGGAGAGCCCCTCTTCTGTGGAAGTGCTGAGTACACCCGAGTTAGAAGTCTTGAATATTTTTCCAGAGTCATCTGACCATATCCATTCTTCTGCAGAAGCACCCAAAAAACCCGAGTCATCAAACATCCCCTCTTCTGCTGAAGTGCTCAATAGACCCGAGTTAGAACTGTTGAATAATCCAGATTTATCTGACCATAGCCCCTCTGCTGCAGAAGCAACTAACAAACCAGAGTCATCCGAATTAAAGATCCCCTCTTCTGCTGACGTACTCAGTACACCTGAGTCAGCTGCAAATAAAATGTCGTCTCCTGCAGAAGCATCAAAAAAACCTGATTCGACTGAAAAGAAAGCCGTGTCCCCTGCAGAAGCATCAAATACTAGAAAGAAAACCCCAAAATCATTGAAGGGCAAAGCTAAAATCGTGAAGAAATCTCTGATTGATAACAGTTTGAAAGGTAGCAAAGCTACTAGTAGTTCGCTGGTTAATGCGAAGAGGAGGAGGTTCAGGAATAAGGGTCCCAAAGAGAGCAGCAACAAAAGAGAAGATGTGAAGAAGGTGAGTTCAAGAAAAGATAAGCAACAGAAAAATACCGATGAACAGCAGACAGATAAGAGCCATCAGGCACAAAAGAATAAAGGCAAGGTGGACGAAGTTGAAAAGAGCCAACAAAAGAATACAGAAAAACGTCATGGGCCGGACAAGAGTGTAAAGAGTGAAATGAATGGCAAAAAACGTGATGAAAAGAGAGAAAAGCTTGGTGGTTTAATATTCATGTGCAGTGGAAAGACAAAGCCAGACTGCTTCCATTATCGTATAATGGGTGTTTCAGTGGGTAAAAAAGACACTGTGTTGGGTATTAAACCTGGGTTGAAGCTTTTTCTCTATGATTTTGACCTTAAGCTTTTGTACGGAGTTTACAAGGCATCCTCTTCAGGTGGCTTAAAACTCGAGCCAAAAGCTTTCGGTGGGGCCTTCCCTGCTCAGGTTCGGTTCAATGTGGAGAAAGATTGCCTTCCTCTGCATGAAAGAGTTTTCAGGAAGGCAATAAAGGAAAACTATGATGAGAAGAACAAGTTCAAAACAGAACTTACAGTTCGACAAGTCAGGAAGCTCACAGCACTTTTCCGACCAGCTCAAGTTCATTTATCTTCTCTACCCACACGTTCCCCACTCAGGGCAGACAATCGGGATAGGGAAACTCATGGGAGGGAGAGAGAAGTGCTGCCACTTTCACATAGGGATGCACATGCTAGAGATCCCTATCCCAATGGCAATGCCAGGAGTTATCCAGCGTTAGCTCATGAAAGGGAGCAGCATGCAAGGAGAGAGGAATTTCCTCGTGATTTGTACCTCTCCGAAAAGGAATATCGAGCTTATGGTCTTCAAGGAGAGAGAAGAAATCTGGCTCCTCGCCCTGTTAGTCCAGCTGTAGAAGTTCAGCACAGAGATTATGAAAGGGACCATTTTGTAAGACAGCCAAACCTCTATAGGGAGGCTGCCTCTGTACATCGAGAAACTGTTCCTTCTGATCCTCTCTACTTGAATGAGAAAGAGTACCCAGCTTATACTGTTGGTGCTAGACATGAGTTGCCTCCTGCAACAGCTGCTGCCGCTGTAGATTTGTATGCAAGGGATCCTTACTATAGTAACTATAGCTATTATGGGGCTAAATCATTGGACCAATATACAGCCCCTCAGAGGAGAGAGGAGGTTCCTTTAGGATCTTACTTAGCTGGTGCAAGGAGAGAATCTTACCTGGTTGAGAATGATCCCATGCAAAGGAGAGCACCTGATCATGTAGAGAGAAGTTTGTACTCCGCACATGCTGCTGCTGCCATTGATGATCGTTTAGAGAGAAGCTTATATTCAGGACGTGCTGTAGGCGATGATCGTTTAGAGAGAAGCTTATACTCGGCACGTGCTGCAGGCGATGATCGTTTAGAGAGAACCTTATACTCGGCACATGCTGCTACTGTTGGTGATGATCGTTTAGGGAGAAGCTTATACTCGGCACGTGCTGCTACTGCTCTGGATGCTGTTGCTCCATACCAGGTGACGAAGCCTGAAGCTCTGCGTACACCAGTTTCATCTCGCTATGCATTTGCTGGTGCATCATACTCCTATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

797

Amino Acids

88.92

Weight (kDa)

9.13

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 341 - 463 1.8e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 1875, 2082
AclWI GGATC 9 cut(s) 12, 139, 505, 1514, 1787, 1892, 1905, 1993, 2027
AcsI RAATTY 1 cut(s) 1595
AcuI CTGAAG 3 cut(s) 423, 1179, 2349
AcvI CACGTG 2 cut(s) 2183, 2279
AfiI CCNNNNNNNGG 7 cut(s) 59, 1246, 1437, 1438, 1753, 1962, 2043
AflII CTTAAG 1 cut(s) 1160
AflIII ACRYGT 1 cut(s) 1424
AhlI ACTAGT 1 cut(s) 731
AjiI CACGTC 1 cut(s) 2138
AjnI CCWGG 4 cut(s) 1126, 1541, 2019, 2313
AloI GAACNNNNNNTCC 2 cut(s) 129, 161
Alw21I GWGCWC 2 cut(s) 411, 2053
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 9 cut(s) 12, 139, 505, 1514, 1787, 1892, 1905, 1993, 2027
AlwNI CAGNNNCTG 1 cut(s) 1871
Ama87I CYCGRG 4 cut(s) 299, 374, 419, 1211
AoxI GGCC 2 cut(s) 956, 1233
ApoI RAATTY 1 cut(s) 1595
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 2 cut(s) 1337, 1782
AspS9I GGNCC 5 cut(s) 779, 956, 1233, 1723, 1942
AsuHPI GGTGA 3 cut(s) 832, 2255, 2329
AvaI CYCGRG 4 cut(s) 299, 374, 419, 1211
AvaII GGWCC 3 cut(s) 779, 1723, 1942
BamHI GGATCC 1 cut(s) 1897
BanII GRGCYC 1 cut(s) 272
BauI CACGAG 1 cut(s) 1602
BbrPI CACGTG 2 cut(s) 2183, 2279
BbsI GAAGAC 1 cut(s) 1637
Bbv12I GWGCWC 2 cut(s) 411, 2053
BccI CCATC 1 cut(s) 888
BceAI ACGGC 1 cut(s) 599
BciT130I CCWGG 4 cut(s) 1128, 1543, 2021, 2315
BclI TGATCA 1 cut(s) 2056
BcoDI GTCTC 1 cut(s) 573
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 5 cut(s) 642, 732, 1515, 1842, 2392
BfrI CTTAAG 1 cut(s) 1160
Bme1390I CCNGG 4 cut(s) 1128, 1543, 2021, 2315
Bme18I GGWCC 3 cut(s) 779, 1723, 1942
BmeT110I CYCGRG 4 cut(s) 299, 374, 419, 1211
BmgBI CACGTC 1 cut(s) 2138
BmgT120I GGNCC 5 cut(s) 779, 956, 1233, 1723, 1942
BmiI GGNNCC 7 cut(s) 780, 781, 1234, 1671, 1724, 1899, 1977
BmrFI CCNGG 4 cut(s) 1128, 1543, 2021, 2315
BmsI GCATC 6 cut(s) 590, 641, 1196, 1495, 2287, 2384
BpiI GAAGAC 1 cut(s) 1637
BplI GAGNNNNNCTC 4 cut(s) 257, 289, 1748, 1780
Bpu10I CCTNAGC 1 cut(s) 1245
BpuEI CTTGAG 1 cut(s) 1383
BsaAI YACGTR 2 cut(s) 2183, 2279
BsaBI GATNNNNATC 1 cut(s) 1524
BsaJI CCNNGG 1 cut(s) 1127
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 1246, 1437, 1438, 1753, 1962, 2043
Bse1I ACTGG 2 cut(s) 125, 2345
Bse3DI GCAATG 1 cut(s) 1543
Bse8I GATNNNNATC 1 cut(s) 1524
BseBI CCWGG 4 cut(s) 1128, 1543, 2021, 2315
BseDI CCNNGG 1 cut(s) 1127
BseGI GGATG 5 cut(s) 387, 497, 1187, 1510, 2302
BseJI GATNNNNATC 1 cut(s) 1524
BseLI CCNNNNNNNGG 7 cut(s) 59, 1246, 1437, 1438, 1753, 1962, 2043
BseMI GCAATG 1 cut(s) 1543
BseMII CTCAG 7 cut(s) 168, 281, 534, 547, 1259, 1450, 1973
BseNI ACTGG 2 cut(s) 125, 2345
BseRI GAGGAG 7 cut(s) 88, 211, 772, 775, 1662, 1978, 1985
BseYI CCCAGC 1 cut(s) 1822
BsgI GTGCAG 1 cut(s) 1060
BshFI GGCC 2 cut(s) 958, 1235
BsiHKAI GWGCWC 2 cut(s) 411, 2053
BsiHKCI CYCGRG 4 cut(s) 299, 374, 419, 1211
BsiSI CCGG 2 cut(s) 248, 959
BslFI GGGAC 3 cut(s) 604, 765, 1736
BslI CCNNNNNNNGG 7 cut(s) 59, 1246, 1437, 1438, 1753, 1962, 2043
BsmAI GTCTC 1 cut(s) 573
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 3 cut(s) 604, 765, 1736
BsnI GGCC 2 cut(s) 958, 1235
BsoBI CYCGRG 4 cut(s) 299, 374, 419, 1211
Bsp1286I GDGCHC 3 cut(s) 272, 411, 2053
Bsp1407I TGTACA 1 cut(s) 1768
BspACI CCGC 2 cut(s) 1875, 2082
BspANI GGCC 2 cut(s) 958, 1235
BspCNI CTCAG 7 cut(s) 169, 282, 535, 546, 1258, 1449, 1972
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 7 cut(s) 780, 781, 1234, 1671, 1724, 1899, 1977
BspMAI CTGCAG 7 cut(s) 109, 232, 358, 475, 577, 628, 2191
BspPI GGATC 9 cut(s) 12, 139, 505, 1514, 1787, 1892, 1905, 1993, 2027
BspTI CTTAAG 1 cut(s) 1160
BsrDI GCAATG 1 cut(s) 1543
BsrGI TGTACA 1 cut(s) 1768
BsrI ACTGG 2 cut(s) 125, 2345
BssECI CCNNGG 1 cut(s) 1127
BssSI CACGAG 1 cut(s) 1602
Bst2BI CACGAG 1 cut(s) 1602
Bst2UI CCWGG 4 cut(s) 1128, 1543, 2021, 2315
Bst4CI ACNGT 7 cut(s) 432, 710, 1111, 1357, 1783, 1834, 2239
Bst6I CTCTTC 5 cut(s) 280, 400, 523, 749, 1198
BstAFI CTTAAG 1 cut(s) 1160
BstAPI GCANNNNNTGC 5 cut(s) 1868, 2090, 2186, 2231, 2282
BstAUI TGTACA 1 cut(s) 1768
BstBAI YACGTR 2 cut(s) 2183, 2279
BstC8I GCNNGC 2 cut(s) 1581, 2191
BstDEI CTNAG 9 cut(s) 167, 177, 290, 533, 543, 1245, 1436, 1959, 1993
BstENI CCTNNNNNAGG 1 cut(s) 57
BstF5I GGATG 5 cut(s) 387, 497, 1187, 1510, 2302
BstMAI GTCTC 1 cut(s) 573
BstNI CCWGG 4 cut(s) 1128, 1543, 2021, 2315
BstNSI RCATGY 4 cut(s) 1514, 1583, 2090, 2231
BstSCI CCNGG 4 cut(s) 1126, 1541, 2019, 2313
BstV2I GAAGAC 1 cut(s) 1637
BstX2I RGATCY 5 cut(s) 144, 510, 1519, 1897, 1985
BstYI RGATCY 5 cut(s) 144, 510, 1519, 1897, 1985
BsuRI GGCC 2 cut(s) 958, 1235
BtgZI GCGATG 2 cut(s) 2163, 2208
BtrI CACGTC 1 cut(s) 2138
BtsCI GGATG 5 cut(s) 387, 497, 1187, 1510, 2302
BtsI GCAGTG 1 cut(s) 1048
BtsIMutI CAGTG 4 cut(s) 132, 1048, 1098, 1107
Cac8I GCNNGC 2 cut(s) 1581, 2191
CaiI CAGNNNCTG 1 cut(s) 1871
CciI TCATGA 1 cut(s) 1564
Cfr13I GGNCC 5 cut(s) 779, 956, 1233, 1723, 1942
CsiI ACCWGGT 2 cut(s) 2019, 2313
DdeI CTNAG 9 cut(s) 167, 177, 290, 533, 543, 1245, 1436, 1959, 1993
Eam1104I CTCTTC 5 cut(s) 280, 400, 523, 749, 1198
EarI CTCTTC 5 cut(s) 280, 400, 523, 749, 1198
Eco24I GRGCYC 1 cut(s) 272
Eco47I GGWCC 3 cut(s) 779, 1723, 1942
Eco57I CTGAAG 3 cut(s) 423, 1179, 2349
Eco72I CACGTG 2 cut(s) 2183, 2279
Eco88I CYCGRG 4 cut(s) 299, 374, 419, 1211
EcoNI CCTNNNNNAGG 1 cut(s) 57
EcoO109I RGGNCCY 2 cut(s) 779, 1233
EcoRII CCWGG 4 cut(s) 1126, 1541, 2019, 2313
EcoT22I ATGCAT 1 cut(s) 2365
EcoT38I GRGCYC 1 cut(s) 272
Esp3I CGTCTC 1 cut(s) 573
FalI AAGNNNNNCTT 4 cut(s) 1260, 1292, 1885, 1917
FaqI GGGAC 3 cut(s) 604, 765, 1736
FbaI TGATCA 1 cut(s) 2056
FokI GGATG 5 cut(s) 374, 484, 1174, 1517, 2309
FriOI GRGCYC 1 cut(s) 272
FspBI CTAG 5 cut(s) 642, 732, 1515, 1842, 2392
GsaI CCCAGC 1 cut(s) 1826
HaeIII GGCC 2 cut(s) 958, 1235
HapII CCGG 2 cut(s) 248, 959
HindIII AAGCTT 7 cut(s) 1016, 1136, 1163, 1221, 2122, 2167, 2263
HpaII CCGG 2 cut(s) 248, 959
HphI GGTGA 3 cut(s) 832, 2255, 2329
Hpy166II GTNNAC 5 cut(s) 296, 539, 910, 1180, 2342
Hpy8I GTNNAC 5 cut(s) 296, 539, 910, 1180, 2342
HpyAV CCTTC 8 cut(s) 53, 159, 661, 811, 1246, 1286, 1297, 1797
HpyCH4III ACNGT 7 cut(s) 432, 710, 1111, 1357, 1783, 1834, 2239
HpyCH4IV ACGT 7 cut(s) 528, 949, 997, 1426, 2137, 2182, 2278
HpyF3I CTNAG 9 cut(s) 167, 177, 290, 533, 543, 1245, 1436, 1959, 1993
HpySE526I ACGT 7 cut(s) 528, 949, 997, 1426, 2137, 2182, 2278
KflI GGGWCCC 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 2056
LmnI GCTCC 3 cut(s) 1573, 1675, 2311
LweI GCATC 6 cut(s) 590, 641, 1196, 1495, 2287, 2384
MabI ACCWGGT 2 cut(s) 2019, 2313
MaeI CTAG 5 cut(s) 642, 732, 1515, 1842, 2392
MaeII ACGT 7 cut(s) 528, 949, 997, 1426, 2137, 2182, 2278
MaeIII GTNAC 2 cut(s) 1910, 2317
MflI RGATCY 5 cut(s) 144, 510, 1519, 1897, 1985
MhlI GDGCHC 3 cut(s) 272, 411, 2053
MluCI AATT 2 cut(s) 503, 1595
MlyI GAGTC 7 cut(s) 58, 212, 260, 338, 386, 503, 554
MmeI TCCRAC 1 cut(s) 1414
Mph1103I ATGCAT 1 cut(s) 2365
MroXI GAANNNNTTC 2 cut(s) 1337, 1782
MseI TTAA 6 cut(s) 506, 747, 1028, 1122, 1161, 1205
MslI CAYNNNNRTG 1 cut(s) 1503
MspA1I CMGCKG 4 cut(s) 551, 1691, 1868, 1877
MspCI CTTAAG 1 cut(s) 1160
MspI CCGG 2 cut(s) 248, 959
MspR9I CCNGG 4 cut(s) 1128, 1543, 2021, 2315
MvaI CCWGG 4 cut(s) 1128, 1543, 2021, 2315
NlaIV GGNNCC 7 cut(s) 780, 781, 1234, 1671, 1724, 1899, 1977
NmeAIII GCCGAG 3 cut(s) 2156, 2201, 2252
NmuCI GTSAC 1 cut(s) 2317
NsiI ATGCAT 1 cut(s) 2365
NspI RCATGY 4 cut(s) 1514, 1583, 2090, 2231
PaeI GCATGC 1 cut(s) 1583
PaeR7I CTCGAG 1 cut(s) 1211
PagI TCATGA 1 cut(s) 1564
PdmI GAANNNNTTC 2 cut(s) 1337, 1782
PfeI GAWTC 3 cut(s) 77, 596, 2012
PleI GAGTC 7 cut(s) 57, 211, 259, 337, 385, 502, 553
PmaCI CACGTG 2 cut(s) 2183, 2279
PmlI CACGTG 2 cut(s) 2183, 2279
PpsI GAGTC 7 cut(s) 57, 211, 259, 337, 385, 502, 553
Ppu21I YACGTR 2 cut(s) 2183, 2279
PpuMI RGGWCCY 1 cut(s) 779
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 4 cut(s) 1126, 1541, 2019, 2313
PspCI CACGTG 2 cut(s) 2183, 2279
PspFI CCCAGC 1 cut(s) 1822
PspGI CCWGG 4 cut(s) 1126, 1541, 2019, 2313
PspN4I GGNNCC 7 cut(s) 780, 781, 1234, 1671, 1724, 1899, 1977
PspPI GGNCC 5 cut(s) 779, 956, 1233, 1723, 1942
PspPPI RGGWCCY 1 cut(s) 779
PspXI VCTCGAGB 1 cut(s) 1211
PstI CTGCAG 7 cut(s) 109, 232, 358, 475, 577, 628, 2191
PstNI CAGNNNCTG 1 cut(s) 1871
PsuI RGATCY 5 cut(s) 144, 510, 1519, 1897, 1985
PvuII CAGCTG 3 cut(s) 551, 1691, 1868
RseI CAYNNNNRTG 1 cut(s) 1503
SaqAI TTAA 6 cut(s) 506, 747, 1028, 1122, 1161, 1205
Sau96I GGNCC 5 cut(s) 779, 956, 1233, 1723, 1942
SchI GAGTC 7 cut(s) 58, 212, 260, 338, 386, 503, 554
ScrFI CCNGG 4 cut(s) 1128, 1543, 2021, 2315
SduI GDGCHC 3 cut(s) 272, 411, 2053
SexAI ACCWGGT 2 cut(s) 2019, 2313
SfaNI GCATC 6 cut(s) 590, 641, 1196, 1495, 2287, 2384
Sfr274I CTCGAG 1 cut(s) 1211
SinI GGWCC 3 cut(s) 779, 1723, 1942
SlaI CTCGAG 1 cut(s) 1211
SmiMI CAYNNNNRTG 1 cut(s) 1503
SmlI CTYRAG 3 cut(s) 1160, 1211, 1398
SmoI CTYRAG 3 cut(s) 1160, 1211, 1398
SpeI ACTAGT 1 cut(s) 731
SphI GCATGC 1 cut(s) 1583
Sse9I AATT 2 cut(s) 503, 1595
SsiI CCGC 2 cut(s) 1875, 2082
SspI AATATT 2 cut(s) 319, 1032
SspMI CTAG 5 cut(s) 642, 732, 1515, 1842, 2392
StyD4I CCNGG 4 cut(s) 1126, 1541, 2019, 2313
TaaI ACNGT 7 cut(s) 432, 710, 1111, 1357, 1783, 1834, 2239
TaiI ACGT 7 cut(s) 531, 952, 1000, 1429, 2140, 2185, 2281
TaqI TCGA 5 cut(s) 599, 1212, 1360, 1633, 1774
TasI AATT 2 cut(s) 503, 1595
TatI WGTACW 5 cut(s) 170, 293, 536, 1768, 2076
TauI GCSGC 1 cut(s) 1877
TfiI GAWTC 3 cut(s) 77, 596, 2012
Tru1I TTAA 6 cut(s) 506, 747, 1028, 1122, 1161, 1205
Tru9I TTAA 6 cut(s) 506, 747, 1028, 1122, 1161, 1205
TscAI CASTG 4 cut(s) 132, 1048, 1098, 1114
TseFI GTSAC 1 cut(s) 2317
Tsp45I GTSAC 1 cut(s) 2317
TspGWI ACGGA 1 cut(s) 1188
TspRI CASTG 4 cut(s) 132, 1048, 1098, 1114
Vha464I CTTAAG 1 cut(s) 1160
VpaK11BI GGWCC 3 cut(s) 779, 1723, 1942
XagI CCTNNNNNAGG 1 cut(s) 57
XapI RAATTY 1 cut(s) 1595
XceI RCATGY 4 cut(s) 1514, 1583, 2090, 2231
XhoI CTCGAG 1 cut(s) 1211
XmnI GAANNNNTTC 2 cut(s) 1337, 1782
XspI CTAG 5 cut(s) 642, 732, 1515, 1842, 2392
Zsp2I ATGCAT 1 cut(s) 2365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.