MD04G1226700.v1.1

NAD(P)-binding Rossmann-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013079
Physical Location & Seq
Reverse (-)
30618312 .. 30621791
3480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1226700.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGCTTTGCTCCTTCCACTCCTCTCTCCGCCTCCACCCCCTCAAAACCCACAAGCCCCTAACCATAATCTGCTCCTCCCAGCCCAAACCACCACCACCACCCAAGAAACGAGCCGCCGCGGACGGCCGAAGACCCTCCCCCAGGTTCGGTAAAAAGCACGGCGCCTCGAGAAGGTCGGTTCTGAAGAAAACGTTCAAGCAGGAGCAGGTGAGCTTCACCACTCCAATTTCCGATGACCCCATTGTTGGTATCATCGGCGGAGGCATGGCGGGCCTCGCCTGTGCTCTCTTTCTGGAGAAACGCGGCGTCCGCTCCACCGTTTTCGACACGGGTATTCATGGTTTGGGAGGAAGATTGGGAACCAGAGTAGTGGATCCTCAGCCTCTGATATTTGATCATGCAGCTCAGTTCTTTACAGCCAGTGATCCTCAGTTTGTTGGGTTGGTTCAAGGGTGGTTGAAGCAAGGTCTGGTTCAAGAATGGCAGGGTGCGGTAGGAGAGCTTGAAGTGGGTGGTAATTTTGTTCCGCTTCCTTCTTCTTCTCCAAGGTACATAGGTGTTAATGGAATGCGCCCCGTTGCTGACTCATTACTTTCTCAGGCTCGTATGATTAATGTGGAGAGACCTTGCTGGATAAGTAAACTCGAGCCCTTCAATGGGATGTGGCATTTGAGTGAGAATGGAAAACCGTATGGGAAGTTTGATGCGATTGTTATCGCACATAATGGTAAATGTGCAAACCGCTTACTTGCATCATCGGGCTTACCACTAGTTTATGGACAAATGAAGAGGCTGGAATTGAGCTCGATATGGGCCCTCCTCGCAGCATTTGAGGATCCACTTCAAATGCCTTTCGAAGGAGCTTTTGTAAAAGGGGTTGATTCCATCTCTTGGATGGCAAATAATACCACAAAACTCCAGGGTTCCCAAAGTAAAGGTCCTCACTGTTGGACCTTTCTTAGTACTGCAGCTTATGGGAAGCGGAACAAGGTTCCACAGGAGAATATACCAACTGCCACGGCAGAGAAGGTGAAGGCTGGCATGCTTTCGGGTGTTGAAGATGCTCTCGGATTACCTAAAGGATCACTTCAGAGCCCCTTTTACACTCGAATCCAGTTATGGGGTGCGGCACTTCCAACTAATACACCCGGCGTCCCCTGCATCTTTGATCCTCTCGGAAGGGCTGGTATATGCGGTGATTGGTTACTGGGTTCAAATGTCGAGTCAGCCGCCTTAAGTGGAATTGCTCTTGCCAATCATATTGCAGACTATTTCCAAAGCAGCGGAGACCGTCCTGAAGAGTTTGCTGTTGGTTTACGCAACGAGTTTCAAAATCATAAAGGACATGATATCGGACAATTTCCCGGGATGGAGTCTGAAACGCAGACGGCTACAGAACAAGCATACGAACTTACCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_8

Protein Analysis

474

Amino Acids

51.17

Weight (kDa)

9.09

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 84 - 144 4.3e-12 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04650
fragaria_vesca FvH4_4g17540
malus_domestica MD04G1226700.v1.1
prunus_persica Prupe.6G345400_v2.0.a1 Prupe.6G345400_v2.0.a1 Prupe.6G345400_v2.0.a1 Prupe.6G345400_v2.0.a1
pyrus_communis pycom04g19990
rosa_chinensis RchiOBHm_Chr4g0421451
rosa_laevigata RLG00000007683
rosa_multiflora Rmu_sc0001962.1_g000014
rosa_roxburghii Rroxscaffold_5G00363720
rosa_rugosa Rorug04G0171300
rosa_samantha Rh4AG232000 Rh4BG234900 Rh4CG246500 Rh4DG230500
rosa_wichuraiana Rw4G020210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 891
AccBSI CCGCTC 1 cut(s) 312
AccII CGCG 2 cut(s) 119, 303
AclI AACGTT 1 cut(s) 191
AclWI GGATC 7 cut(s) 368, 381, 419, 830, 843, 1090, 1163
AcoI YGGCCR 1 cut(s) 124
AcuI CTGAAG 3 cut(s) 203, 1073, 1317
AcyI GRCGYC 3 cut(s) 162, 306, 1152
AfaI GTAC 2 cut(s) 551, 964
AfiI CCNNNNNNNGG 9 cut(s) 141, 146, 171, 245, 656, 657, 857, 891, 1120
AflII CTTAAG 1 cut(s) 1234
AhlI ACTAGT 1 cut(s) 769
AjnI CCWGG 2 cut(s) 140, 918
AluBI AGCT 6 cut(s) 213, 404, 502, 804, 863, 971
AluI AGCT 6 cut(s) 213, 404, 502, 804, 863, 971
Alw21I GWGCWC 2 cut(s) 286, 806
Alw26I GTCTC 2 cut(s) 616, 1281
AlwI GGATC 7 cut(s) 368, 381, 419, 830, 843, 1090, 1163
AlwNI CAGNNNCTG 1 cut(s) 385
Ama87I CYCGRG 3 cut(s) 166, 644, 1364
AoxI GGCC 3 cut(s) 124, 271, 813
ApaI GGGCCC 1 cut(s) 817
ApeKI GCWGC 4 cut(s) 401, 824, 968, 1281
ArsI GACNNNNNNTTYG 4 cut(s) 922, 954, 1208, 1240
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 191
AspLEI GCGC 2 cut(s) 164, 573
AspS9I GGNCC 5 cut(s) 271, 813, 814, 938, 951
AsuC2I CCSGG 3 cut(s) 1149, 1365, 1366
AsuHPI GGTGA 4 cut(s) 208, 220, 1042, 1208
AsuII TTCGAA 1 cut(s) 855
AvaI CYCGRG 3 cut(s) 166, 644, 1364
AvaII GGWCC 2 cut(s) 938, 951
BaeGI GKGCMC 1 cut(s) 817
BaeI ACNNNNGTAYC 2 cut(s) 541, 574
BamHI GGATCC 2 cut(s) 373, 835
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 4 cut(s) 651, 806, 817, 1097
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 2 cut(s) 286, 806
BbvCI CCTCAGC 1 cut(s) 378
BbvI GCAGC 4 cut(s) 413, 836, 980, 1293
BccI CCATC 3 cut(s) 889, 893, 1363
BceAI ACGGC 4 cut(s) 139, 175, 1035, 1404
BciT130I CCWGG 2 cut(s) 142, 920
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 3 cut(s) 1149, 1365, 1366
BcoDI GTCTC 2 cut(s) 616, 1281
BcuI ACTAGT 1 cut(s) 769
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 2 cut(s) 966, 1392
BfoI RGCGCY 1 cut(s) 165
BfrI CTTAAG 1 cut(s) 1234
BfuAI ACCTGC 1 cut(s) 196
BisI GCNGC 9 cut(s) 114, 117, 304, 402, 825, 969, 1128, 1230, 1282
BlsI GCNGC 9 cut(s) 115, 118, 305, 403, 826, 970, 1129, 1231, 1283
BmcAI AGTACT 1 cut(s) 964
Bme1390I CCNGG 5 cut(s) 142, 920, 1149, 1365, 1366
Bme18I GGWCC 2 cut(s) 938, 951
BmeT110I CYCGRG 3 cut(s) 166, 644, 1364
BmgT120I GGNCC 5 cut(s) 271, 813, 814, 938, 951
BmiI GGNNCC 7 cut(s) 163, 361, 375, 815, 837, 925, 993
BmrFI CCNGG 5 cut(s) 142, 920, 1149, 1365, 1366
BmrI ACTGGG 1 cut(s) 1217
BmsI GCATC 4 cut(s) 694, 761, 1051, 1170
BmuI ACTGGG 1 cut(s) 1217
BpiI GAAGAC 1 cut(s) 136
BpmI CTGGAG 2 cut(s) 314, 902
Bpu10I CCTNAGC 1 cut(s) 378
Bpu14I TTCGAA 1 cut(s) 855
BpuMI CCSGG 3 cut(s) 1149, 1365, 1366
BsaBI GATNNNNATC 1 cut(s) 713
BsaHI GRCGYC 3 cut(s) 162, 306, 1152
BsaI GGTCTC 2 cut(s) 616, 1281
BsaJI CCNNGG 6 cut(s) 117, 140, 545, 919, 1017, 1364
BsaXI ACNNNNNCTCC 2 cut(s) 852, 882
Bsc4I CCNNNNNNNGG 9 cut(s) 141, 146, 171, 245, 656, 657, 857, 891, 1120
Bse1I ACTGG 3 cut(s) 420, 1114, 1212
Bse8I GATNNNNATC 1 cut(s) 713
BseBI CCWGG 2 cut(s) 142, 920
BseDI CCNNGG 6 cut(s) 117, 140, 545, 919, 1017, 1364
BseGI GGATG 3 cut(s) 666, 900, 1374
BseJI GATNNNNATC 1 cut(s) 713
BseLI CCNNNNNNNGG 9 cut(s) 141, 146, 171, 245, 656, 657, 857, 891, 1120
BseMII CTCAG 4 cut(s) 392, 419, 443, 611
BseNI ACTGG 3 cut(s) 420, 1114, 1212
BseRI GAGGAG 3 cut(s) 10, 64, 809
BseSI GKGCMC 1 cut(s) 817
BseX3I CGGCCG 1 cut(s) 124
BseXI GCAGC 4 cut(s) 413, 836, 980, 1293
BseYI CCCAGC 1 cut(s) 78
Bsh1236I CGCG 2 cut(s) 119, 303
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 3 cut(s) 126, 273, 815
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 2 cut(s) 286, 806
BsiHKCI CYCGRG 3 cut(s) 166, 644, 1364
BsiSI CCGG 2 cut(s) 1149, 1365
BslFI GGGAC 1 cut(s) 1139
BslI CCNNNNNNNGG 9 cut(s) 141, 146, 171, 245, 656, 657, 857, 891, 1120
BsmAI GTCTC 2 cut(s) 616, 1281
BsmFI GGGAC 1 cut(s) 1139
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 3 cut(s) 126, 273, 815
Bso31I GGTCTC 2 cut(s) 616, 1281
BsoBI CYCGRG 3 cut(s) 166, 644, 1364
Bsp119I TTCGAA 1 cut(s) 855
Bsp120I GGGCCC 1 cut(s) 813
Bsp1286I GDGCHC 5 cut(s) 286, 651, 806, 817, 1097
Bsp143I GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
BspANI GGCC 3 cut(s) 126, 273, 815
BspCNI CTCAG 4 cut(s) 391, 418, 442, 610
BspFNI CGCG 2 cut(s) 119, 303
BspLI GGNNCC 7 cut(s) 163, 361, 375, 815, 837, 925, 993
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 7 cut(s) 368, 381, 419, 830, 843, 1090, 1163
BspT104I TTCGAA 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 1234
BspTNI GGTCTC 2 cut(s) 616, 1281
BsrBI CCGCTC 1 cut(s) 312
BsrI ACTGG 3 cut(s) 420, 1114, 1212
BssECI CCNNGG 6 cut(s) 117, 140, 545, 919, 1017, 1364
BssMI GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
BssNI GRCGYC 3 cut(s) 162, 306, 1152
BssT1I CCWWGG 1 cut(s) 545
Bst2UI CCWGG 2 cut(s) 142, 920
Bst4CI ACNGT 4 cut(s) 319, 690, 947, 1292
Bst6I CTCTTC 2 cut(s) 782, 1293
BstACI GRCGYC 3 cut(s) 162, 306, 1152
BstAFI CTTAAG 1 cut(s) 1234
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 3 cut(s) 271, 1039, 1043
BstDEI CTNAG 5 cut(s) 378, 405, 429, 597, 959
BstDSI CCRYGG 2 cut(s) 117, 1017
BstENI CCTNNNNNAGG 3 cut(s) 139, 169, 855
BstF5I GGATG 3 cut(s) 666, 900, 1374
BstFNI CGCG 2 cut(s) 119, 303
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 573
BstKTI GATC 6 cut(s) 376, 397, 427, 838, 1085, 1171
BstMAI GTCTC 2 cut(s) 616, 1281
BstMBI GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 5 cut(s) 270, 275, 309, 821, 1158
BstNI CCWGG 2 cut(s) 142, 920
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 5 cut(s) 140, 918, 1147, 1363, 1364
BstSFI CTRYAG 2 cut(s) 966, 1392
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 2 cut(s) 119, 303
BstV1I GCAGC 4 cut(s) 413, 836, 980, 1293
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 2 cut(s) 373, 835
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 2 cut(s) 373, 835
BstZI CGGCCG 1 cut(s) 124
BsuRI GGCC 3 cut(s) 126, 273, 815
BtgI CCRYGG 2 cut(s) 117, 1017
BtsCI GGATG 3 cut(s) 666, 900, 1374
BtsIMutI CAGTG 2 cut(s) 427, 943
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 271, 1039, 1043
CaiI CAGNNNCTG 1 cut(s) 385
CfoI GCGC 2 cut(s) 164, 573
Cfr13I GGNCC 5 cut(s) 271, 813, 814, 938, 951
Cfr42I CCGCGG 1 cut(s) 120
Cfr9I CCCGGG 1 cut(s) 1364
CseI GACGC 2 cut(s) 295, 1141
Csp6I GTAC 2 cut(s) 550, 963
CviAII CATG 5 cut(s) 265, 338, 398, 1042, 1346
CviQI GTAC 2 cut(s) 550, 963
DdeI CTNAG 5 cut(s) 378, 405, 429, 597, 959
DinI GGCGCC 1 cut(s) 163
DpnI GATC 6 cut(s) 375, 396, 426, 837, 1084, 1170
DpnII GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
EaeI YGGCCR 1 cut(s) 124
EagI CGGCCG 1 cut(s) 124
Eam1104I CTCTTC 2 cut(s) 782, 1293
EarI CTCTTC 2 cut(s) 782, 1293
EciI GGCGGA 2 cut(s) 17, 273
Ecl136II GAGCTC 1 cut(s) 804
EclXI CGGCCG 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 545
Eco24I GRGCYC 4 cut(s) 651, 806, 817, 1097
Eco31I GGTCTC 2 cut(s) 616, 1281
Eco32I GATATC 1 cut(s) 1351
Eco47I GGWCC 2 cut(s) 938, 951
Eco52I CGGCCG 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 804
Eco57I CTGAAG 3 cut(s) 203, 1073, 1317
Eco88I CYCGRG 3 cut(s) 166, 644, 1364
EcoICRI GAGCTC 1 cut(s) 804
EcoNI CCTNNNNNAGG 3 cut(s) 139, 169, 855
EcoO109I RGGNCCY 2 cut(s) 814, 938
EcoRII CCWGG 2 cut(s) 140, 918
EcoRV GATATC 1 cut(s) 1351
EcoT14I CCWWGG 1 cut(s) 545
EcoT38I GRGCYC 4 cut(s) 651, 806, 817, 1097
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 545
FaeI CATG 5 cut(s) 268, 341, 401, 1045, 1349
FalI AAGNNNNNCTT 2 cut(s) 1071, 1103
FaqI GGGAC 1 cut(s) 1139
FatI CATG 5 cut(s) 264, 337, 397, 1041, 1345
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 9 cut(s) 114, 117, 304, 402, 825, 969, 1128, 1230, 1282
FokI GGATG 3 cut(s) 673, 907, 1381
FriOI GRGCYC 4 cut(s) 651, 806, 817, 1097
Fsp4HI GCNGC 9 cut(s) 114, 117, 304, 402, 825, 969, 1128, 1230, 1282
FspBI CTAG 1 cut(s) 770
GlaI GCGC 2 cut(s) 163, 572
GluI GCNGC 9 cut(s) 114, 117, 304, 402, 825, 969, 1128, 1230, 1282
GsaI CCCAGC 1 cut(s) 82
GsuI CTGGAG 2 cut(s) 314, 902
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 126, 273, 815
HapII CCGG 2 cut(s) 1149, 1365
HgaI GACGC 2 cut(s) 295, 1141
HhaI GCGC 2 cut(s) 164, 573
Hin1I GRCGYC 3 cut(s) 162, 306, 1152
Hin1II CATG 5 cut(s) 268, 341, 401, 1045, 1349
Hin6I GCGC 2 cut(s) 162, 571
HinP1I GCGC 2 cut(s) 162, 571
HinfI GANTC 5 cut(s) 584, 881, 1110, 1223, 1373
HpaII CCGG 2 cut(s) 1149, 1365
HphI GGTGA 4 cut(s) 208, 220, 1042, 1208
Hpy166II GTNNAC 2 cut(s) 641, 1316
Hpy188I TCNGA 8 cut(s) 183, 232, 387, 1070, 1092, 1178, 1355, 1378
Hpy188III TCNNGA 4 cut(s) 168, 293, 476, 1295
Hpy8I GTNNAC 2 cut(s) 641, 1316
HpyAV CCTTC 8 cut(s) 22, 165, 543, 661, 851, 1021, 1027, 1173
HpyCH4III ACNGT 4 cut(s) 319, 690, 947, 1292
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 6 cut(s) 401, 737, 752, 968, 1161, 1265
HpyF10VI GCNNNNNNNGC 5 cut(s) 270, 275, 309, 821, 1158
HpyF3I CTNAG 5 cut(s) 378, 405, 429, 597, 959
HpySE526I ACGT 1 cut(s) 191
Hsp92I GRCGYC 3 cut(s) 162, 306, 1152
Hsp92II CATG 5 cut(s) 268, 341, 401, 1045, 1349
HspAI GCGC 2 cut(s) 162, 571
KasI GGCGCC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 394
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
LmnI GCTCC 5 cut(s) 14, 77, 202, 317, 860
Lsp1109I GCAGC 4 cut(s) 413, 836, 980, 1293
LweI GCATC 4 cut(s) 694, 761, 1051, 1170
MaeI CTAG 1 cut(s) 770
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 1203
MalI GATC 6 cut(s) 375, 396, 426, 837, 1084, 1170
MbiI CCGCTC 1 cut(s) 312
MboI GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
MboII GAAGA 8 cut(s) 141, 196, 363, 528, 531, 799, 1070, 1310
MflI RGATCY 2 cut(s) 373, 835
MhlI GDGCHC 5 cut(s) 286, 651, 806, 817, 1097
MluCI AATT 5 cut(s) 225, 517, 797, 1242, 1358
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 3 cut(s) 578, 1232, 1382
MmeI TCCRAC 2 cut(s) 929, 1160
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 4 cut(s) 561, 612, 1235, 1420
MslI CAYNNNNRTG 1 cut(s) 672
MspA1I CMGCKG 2 cut(s) 119, 1284
MspCI CTTAAG 1 cut(s) 1234
MspI CCGG 2 cut(s) 1149, 1365
MspR9I CCNGG 5 cut(s) 142, 920, 1149, 1365, 1366
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 2 cut(s) 142, 920
MvnI CGCG 2 cut(s) 119, 303
MwoI GCNNNNNNNGC 5 cut(s) 270, 275, 309, 821, 1158
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 3 cut(s) 1149, 1365, 1366
NdeII GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
NlaIII CATG 5 cut(s) 268, 341, 401, 1045, 1349
NlaIV GGNNCC 7 cut(s) 163, 361, 375, 815, 837, 925, 993
NspI RCATGY 1 cut(s) 1045
NspV TTCGAA 1 cut(s) 855
PaeI GCATGC 1 cut(s) 1045
PaeR7I CTCGAG 2 cut(s) 166, 644
PaqCI CACCTGC 1 cut(s) 196
PctI GAATGC 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 881, 1110
PflMI CCANNNNNTGG 1 cut(s) 891
PkrI GCNGC 9 cut(s) 115, 118, 305, 403, 826, 970, 1129, 1231, 1283
PleI GAGTC 3 cut(s) 578, 1231, 1381
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 3 cut(s) 578, 1231, 1381
PpuMI RGGWCCY 1 cut(s) 938
PshBI ATTAAT 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 806
Psp1406I AACGTT 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 2 cut(s) 140, 918
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 2 cut(s) 140, 918
PspN4I GGNNCC 7 cut(s) 163, 361, 375, 815, 837, 925, 993
PspOMI GGGCCC 1 cut(s) 813
PspPI GGNCC 5 cut(s) 271, 813, 814, 938, 951
PspPPI RGGWCCY 1 cut(s) 938
PspXI VCTCGAGB 1 cut(s) 644
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 2 cut(s) 373, 835
RsaI GTAC 2 cut(s) 551, 964
RsaNI GTAC 2 cut(s) 550, 963
RseI CAYNNNNRTG 1 cut(s) 672
SacI GAGCTC 1 cut(s) 806
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 4 cut(s) 561, 612, 1235, 1420
SatI GCNGC 9 cut(s) 114, 117, 304, 402, 825, 969, 1128, 1230, 1282
Sau3AI GATC 6 cut(s) 373, 394, 424, 835, 1082, 1168
Sau96I GGNCC 5 cut(s) 271, 813, 814, 938, 951
ScaI AGTACT 1 cut(s) 964
SchI GAGTC 3 cut(s) 578, 1232, 1382
ScrFI CCNGG 5 cut(s) 142, 920, 1149, 1365, 1366
SduI GDGCHC 5 cut(s) 286, 651, 806, 817, 1097
SfaNI GCATC 4 cut(s) 694, 761, 1051, 1170
SfcI CTRYAG 2 cut(s) 966, 1392
SfoI GGCGCC 1 cut(s) 163
Sfr274I CTCGAG 2 cut(s) 166, 644
Sfr303I CCGCGG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 855
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 2 cut(s) 938, 951
SlaI CTCGAG 2 cut(s) 166, 644
SmaI CCCGGG 1 cut(s) 1366
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 3 cut(s) 166, 644, 1234
SmoI CTYRAG 3 cut(s) 166, 644, 1234
SpeI ACTAGT 1 cut(s) 769
SphI GCATGC 1 cut(s) 1045
Sse9I AATT 5 cut(s) 225, 517, 797, 1242, 1358
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 1 cut(s) 770
SstI GAGCTC 1 cut(s) 806
StyD4I CCNGG 5 cut(s) 140, 918, 1147, 1363, 1364
StyI CCWWGG 1 cut(s) 545
TaaI ACNGT 4 cut(s) 319, 690, 947, 1292
TaiI ACGT 1 cut(s) 194
TaqI TCGA 7 cut(s) 167, 324, 645, 806, 855, 1108, 1221
TasI AATT 5 cut(s) 225, 517, 797, 1242, 1358
TatI WGTACW 1 cut(s) 962
TauI GCSGC 5 cut(s) 116, 119, 306, 1130, 1232
TfiI GAWTC 2 cut(s) 881, 1110
Tru1I TTAA 4 cut(s) 561, 612, 1235, 1420
Tru9I TTAA 4 cut(s) 561, 612, 1235, 1420
TscAI CASTG 2 cut(s) 427, 950
TseI GCWGC 4 cut(s) 401, 824, 968, 1281
TspDTI ATGAA 2 cut(s) 326, 800
TspMI CCCGGG 1 cut(s) 1364
TspRI CASTG 2 cut(s) 427, 950
Van91I CCANNNNNTGG 1 cut(s) 891
Vha464I CTTAAG 1 cut(s) 1234
VpaK11BI GGWCC 2 cut(s) 938, 951
VspI ATTAAT 1 cut(s) 612
XagI CCTNNNNNAGG 3 cut(s) 139, 169, 855
XceI RCATGY 1 cut(s) 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XhoI CTCGAG 2 cut(s) 166, 644
XmaI CCCGGG 1 cut(s) 1364
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 1 cut(s) 770
ZrmI AGTACT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.