Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0007921

Physical Location & Seq

Genomic Coordinates Forward (+)

30648762 .. 30653696

Gene Structure 4935 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1227000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGTTGGTTCGTCATTGGGTACAATTTATGGCTGAAGCTGATAAACTCTATGGGGAACTTGATGGAGGTGAAGTACCTGTGACCGTGGCTGAAATCGATGGTGAGATTGAGAATGCACAGCCACTGGTAGAAGACCAGGCTACGGCTCTAGACCCAGCTCCGGAGCTGACGGAGGCCAATGTACAGGGGTATGAACAAGAACATGGAGTGACATTTGCACAAGAACAAGAAGATGCATTTGCACAGTTCCAGGCACAGCAGCAGGAAGATGCACTTGCACAGTTCCAGGCACAGAAGCAGGAAGACGCACTGGCTCAGTTCCAGGCACAGGAGCAGGAAGAGGCACTGGCCCAGTTCCAGGCGCAGGAACAAGTAGAGTTATCAGTAGAAGTACAAGAGGAGGAGGCGCAATTACCTGAACAGAACCAAGAGCGTCATGAAGATGCTGCTGTGGCGGGTGGAGGTGAGAAGAAGTGGCCTGGATGGCCAGGAGAGAGTGTTTTCCGGATGCTGGTTCCTGCGCAAAAGGTTGGCAGTATAATTGGACGCAAAGGGGAGTTCATTAAGAAAATAGTTGAGGAGACAAGAGCTCGCATTAAAATTCTTGATGGTCCCCCGGGAACAACTGAAAGAGCTGTAATGGTTTCTGGCAAGGAGGAGCCAGATTCTTCTCTGCCCCCAGCAATGGATGGCCTTTTGAGGGTTCACAAGCGTATTATTGATGGTTTGGATGGTGATTCTTCTCATGCTCCACCGGGCATGGGTGGCAAAGTATCCACAAAGCTGCTGGTCGCAGCTTCACAGGCAGGAAGCTTGATAGGGAAACAGGGAGGAACTGTTAAATCCATTCAAGAAGCATCTAACTGCATAGTTAGAGTTCTTGGATCAGAAGACCTACCGATTTTTGCTCTTCAAGATGACAGAGTGGTTGAAGTAGTGGGTGAGGGTGTTGGTGTGCACAAAGCAATTGAACTAATTGCAGCTCATCTTAGGAAGTTTTTAGTTGACCGCAGTATAATTCCAATATTTGAAATGCATATGCAAATGGCAAATCCTCAGATGGATCATGGGCCACCTCATCAAAATTGGGGTCCACCTCAAGGTCATCCCCACAATGCTGGTCATGGGGGTCCTGGATATGGGCCGCCTAATCCCCAATACATGCCACCTCCTCGGCAGCTTGACAATTACTATCCACCGGCTGACATGCCTCCACCTATGGAGAAACAGCCTCATCATGGTATATCGGCTTATGGAAGAGAAGCTCCAATGGGTTCCCACCAATCATCAAATGCTCAGTCCGCACCAGGAATGGTCACACAGATCACCAAGCAATTGCAAATTCCGCTGTCATATGCGGATGCTGTAATTGGCACAGCTGGTGCAAGTATAAGCTATATTCGACGCGCTAGTGGGGCAACTGTTACGATACAAGAGACTAGGGGTGTACCTGGGGAGATGACTGTTGAAATCAGTGGAAGTGCTCCCGAAGTTCAAGCAGCTCAGCAACTGATACAGGCAATGCTCAATTTCATGGCTGATGCTGGGGCACCGCAGCCGACCCCAACTCCTGGCTCTGTGGACCAAGGTTATAACAACTATGCAACTCATGGTTCGGTGTATGCATCTCCCCCATCTAATCAAGGACATGCAGGACAACATGGGGGCTATGGCTCAGCCTACGGCTCGCATTATGGGTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009909 GO:0009911 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0097159 GO:1901363 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

568

Amino Acids

60.85

Weight (kDa)

4.86

Isoelectric Point (pI)

47.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	168 - 220	1.6e-14	KH domain
KH_1	PF00013	260 - 327	1.5e-13	KH domain
KH_1	PF00013	443 - 506	6.6e-12	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04610 AT3G04610 AT3G04610 AT3G04610
fragaria_vesca	FvH4_6g02580 FvH4_6g02580 FvH4_6g02580 FvH4_6g02580
malus_domestica	MD04G1227000.v1.1 MD12G1244000.v1.1
prunus_persica	Prupe.6G345700_v2.0.a1 Prupe.6G345700_v2.0.a1
pyrus_communis	pycom04g20030 pycom12g22300
rosa_chinensis	RchiOBHm_Chr3g0450491
rosa_laevigata	RLG00000025727
rosa_multiflora	Rmu_sc0005951.1_g000006
rosa_roxburghii	Rroxscaffold_6G00428170
rosa_rugosa	Rorug02G0628000
rosa_samantha	Rh3AG028700 Rh3BG029200 Rh3CG028000 Rh3DG028900
rosa_wichuraiana	Rw3G002210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1593
Acc16I	TGCGCA	1 cut(s)	522
AccB1I	GGYRCC	1 cut(s)	1549
AccB7I	CCANNNNNTGG	1 cut(s)	1571
AccII	CGCG	1 cut(s)	1407
AccIII	TCCGGA	2 cut(s)	160, 504
AciI	CCGC	7 cut(s)	455, 1009, 1145, 1302, 1346, 1358, 1553
AclWI	GGATC	2 cut(s)	892, 1071
AcoI	YGGCCR	1 cut(s)	485
AcsI	RAATTY	2 cut(s)	600, 1341
AcuI	CTGAAG	1 cut(s)	54
AfaI	GTAC	6 cut(s)	21, 75, 183, 393, 1449, 1700
AgsI	TTSAA	7 cut(s)	851, 914, 932, 971, 1031, 1469, 1496
Alw21I	GWGCWC	3 cut(s)	592, 960, 1486
Alw26I	GTCTC	2 cut(s)	575, 1430
Alw44I	GTGCAC	1 cut(s)	956
AlwI	GGATC	2 cut(s)	892, 1071
AlwNI	CAGNNNCTG	2 cut(s)	124, 1510
Ama87I	CYCGRG	1 cut(s)	616
Aor13HI	TCCGGA	2 cut(s)	160, 504
AoxI	GGCC	7 cut(s)	174, 348, 476, 485, 691, 1070, 1142
ApaLI	GTGCAC	1 cut(s)	956
ApeKI	GCWGC	8 cut(s)	259, 446, 784, 794, 980, 1177, 1499, 1555
ApoI	RAATTY	2 cut(s)	600, 1341
ArsI	GACNNNNNNTTYG	4 cut(s)	1075, 1107, 1283, 1315
AspLEI	GCGC	4 cut(s)	364, 409, 523, 1409
AspS9I	GGNCC	7 cut(s)	349, 611, 1070, 1091, 1130, 1142, 1582
AsuC2I	CCSGG	3 cut(s)	617, 618, 756
AsuHPI	GGTGA	6 cut(s)	80, 113, 476, 746, 953, 1318
AvaI	CYCGRG	1 cut(s)	616
AvaII	GGWCC	4 cut(s)	611, 1091, 1130, 1582
BaeGI	GKGCMC	2 cut(s)	960, 1552
BalI	TGGCCA	1 cut(s)	487
BanI	GGYRCC	1 cut(s)	1549
BanII	GRGCYC	1 cut(s)	592
BbsI	GAAGAC	3 cut(s)	138, 309, 897
Bbv12I	GWGCWC	3 cut(s)	592, 960, 1486
BbvI	GCAGC	8 cut(s)	271, 433, 771, 806, 992, 1189, 1511, 1567
BccI	CCATC	9 cut(s)	56, 92, 477, 602, 683, 716, 725, 1054, 1642
BceAI	ACGGC	2 cut(s)	159, 1699
BciVI	GTATCC	1 cut(s)	784
BcnI	CCSGG	3 cut(s)	617, 618, 756
BcoDI	GTCTC	2 cut(s)	575, 1430
BfaI	CTAG	4 cut(s)	149, 1410, 1440, 1702
BfuI	GTATCC	1 cut(s)	784
BglI	GCCNNNNNGGC	1 cut(s)	484
BisI	GCNGC	9 cut(s)	260, 447, 785, 795, 981, 1145, 1178, 1500, 1556
BlpI	GCTNAGC	2 cut(s)	1503, 1675
BlsI	GCNGC	9 cut(s)	261, 448, 786, 796, 982, 1146, 1179, 1501, 1557
Bme18I	GGWCC	4 cut(s)	611, 1091, 1130, 1582
BmeT110I	CYCGRG	1 cut(s)	616
BmgT120I	GGNCC	7 cut(s)	349, 611, 1070, 1091, 1130, 1142, 1582
BmiI	GGNNCC	7 cut(s)	516, 613, 660, 1092, 1131, 1276, 1551
BmrI	ACTGGG	1 cut(s)	346
BmsI	GCATC	8 cut(s)	223, 259, 433, 498, 866, 1351, 1531, 1634
BmuI	ACTGGG	1 cut(s)	346
BpiI	GAAGAC	3 cut(s)	138, 309, 897
Bpu1102I	GCTNAGC	2 cut(s)	1503, 1675
BpuEI	CTTGAG	1 cut(s)	1083
BpuMI	CCSGG	3 cut(s)	617, 618, 756
Bsa29I	ATCGAT	1 cut(s)	96
BsaJI	CCNNGG	6 cut(s)	84, 615, 616, 1172, 1451, 1585
BsaWI	WCCGGW	2 cut(s)	160, 504
BsaXI	ACNNNNNCTCC	8 cut(s)	57, 87, 483, 513, 822, 852, 1552, 1582
Bse118I	RCCGGY	1 cut(s)	1198
Bse1I	ACTGG	4 cut(s)	129, 315, 351, 352
Bse3DI	GCAATG	2 cut(s)	690, 1527
BseAI	TCCGGA	2 cut(s)	160, 504
BseCI	ATCGAT	1 cut(s)	96
BseDI	CCNNGG	6 cut(s)	84, 615, 616, 1172, 1451, 1585
BseGI	GGATG	6 cut(s)	488, 513, 694, 736, 1105, 1366
BseMI	GCAATG	2 cut(s)	690, 1527
BseMII	CTCAG	5 cut(s)	329, 1070, 1310, 1517, 1689
BseNI	ACTGG	4 cut(s)	129, 315, 351, 352
BseRI	GAGGAG	5 cut(s)	413, 416, 593, 671, 1161
BseSI	GKGCMC	2 cut(s)	960, 1552
BseXI	GCAGC	8 cut(s)	271, 433, 771, 806, 992, 1189, 1511, 1567
BseYI	CCCAGC	3 cut(s)	154, 679, 1544
Bsh1236I	CGCG	1 cut(s)	1407
BshFI	GGCC	7 cut(s)	176, 350, 478, 487, 693, 1072, 1144
BshNI	GGYRCC	1 cut(s)	1549
BshVI	ATCGAT	1 cut(s)	96
BsiHKAI	GWGCWC	3 cut(s)	592, 960, 1486
BsiHKCI	CYCGRG	1 cut(s)	616
BsiSI	CCGG	5 cut(s)	161, 505, 617, 755, 1199
BslFI	GGGAC	1 cut(s)	597
BsmAI	GTCTC	2 cut(s)	575, 1430
BsmFI	GGGAC	1 cut(s)	597
BsmI	GAATGC	1 cut(s)	118
BsnI	GGCC	7 cut(s)	176, 350, 478, 487, 693, 1072, 1144
BsoBI	CYCGRG	1 cut(s)	616
Bsp1286I	GDGCHC	4 cut(s)	592, 960, 1486, 1552
Bsp13I	TCCGGA	2 cut(s)	160, 504
Bsp1407I	TGTACA	1 cut(s)	181
Bsp143I	GATC	3 cut(s)	884, 1063, 1323
Bsp1720I	GCTNAGC	2 cut(s)	1503, 1675
BspACI	CCGC	7 cut(s)	455, 1009, 1145, 1302, 1346, 1358, 1553
BspANI	GGCC	7 cut(s)	176, 350, 478, 487, 693, 1072, 1144
BspCNI	CTCAG	5 cut(s)	328, 1069, 1309, 1516, 1688
BspDI	ATCGAT	1 cut(s)	96
BspEI	TCCGGA	2 cut(s)	160, 504
BspFNI	CGCG	1 cut(s)	1407
BspHI	TCATGA	1 cut(s)	436
BspLI	GGNNCC	7 cut(s)	516, 613, 660, 1092, 1131, 1276, 1551
BspPI	GGATC	2 cut(s)	892, 1071
BspQI	GCTCTTC	1 cut(s)	915
BspT107I	GGYRCC	1 cut(s)	1549
BsrDI	GCAATG	2 cut(s)	690, 1527
BsrFI	RCCGGY	1 cut(s)	1198
BsrGI	TGTACA	1 cut(s)	181
BsrI	ACTGG	4 cut(s)	129, 315, 351, 352
BssAI	RCCGGY	1 cut(s)	1198
BssECI	CCNNGG	6 cut(s)	84, 615, 616, 1172, 1451, 1585
BssMI	GATC	3 cut(s)	884, 1063, 1323
BssT1I	CCWWGG	1 cut(s)	1585
Bst4CI	ACNGT	6 cut(s)	85, 246, 282, 838, 1423, 1465
Bst6I	CTCTTC	3 cut(s)	333, 915, 1252
BstAUI	TGTACA	1 cut(s)	181
BstC8I	GCNNGC	2 cut(s)	592, 1688
BstDEI	CTNAG	6 cut(s)	315, 989, 1056, 1296, 1503, 1675
BstDSI	CCRYGG	1 cut(s)	84
BstF5I	GGATG	6 cut(s)	488, 513, 694, 736, 1105, 1366
BstFNI	CGCG	1 cut(s)	1407
BstHHI	GCGC	4 cut(s)	364, 409, 523, 1409
BstKTI	GATC	3 cut(s)	887, 1066, 1326
BstMAI	GTCTC	2 cut(s)	575, 1430
BstMBI	GATC	3 cut(s)	884, 1063, 1323
BstMWI	GCNNNNNNNGC	7 cut(s)	452, 484, 765, 803, 1301, 1345, 1415
BstNSI	RCATGY	3 cut(s)	1165, 1210, 1652
BstSLI	GKGCMC	2 cut(s)	960, 1552
BstUI	CGCG	1 cut(s)	1407
BstV1I	GCAGC	8 cut(s)	271, 433, 771, 806, 992, 1189, 1511, 1567
BstV2I	GAAGAC	3 cut(s)	138, 309, 897
BstXI	CCANNNNNNTGG	1 cut(s)	1118
Bsu15I	ATCGAT	1 cut(s)	96
BsuI	GTATCC	1 cut(s)	784
BsuRI	GGCC	7 cut(s)	176, 350, 478, 487, 693, 1072, 1144
BsuTUI	ATCGAT	1 cut(s)	96
BtgI	CCRYGG	1 cut(s)	84
BtsCI	GGATG	6 cut(s)	488, 513, 694, 736, 1105, 1366
BtsIMutI	CAGTG	4 cut(s)	122, 308, 344, 1480
Cac8I	GCNNGC	2 cut(s)	592, 1688
CaiI	CAGNNNCTG	2 cut(s)	124, 1510
CciI	TCATGA	1 cut(s)	436
CfoI	GCGC	4 cut(s)	364, 409, 523, 1409
Cfr10I	RCCGGY	1 cut(s)	1198
Cfr13I	GGNCC	7 cut(s)	349, 611, 1070, 1091, 1130, 1142, 1582
Cfr9I	CCCGGG	1 cut(s)	616
ClaI	ATCGAT	1 cut(s)	96
CseI	GACGC	4 cut(s)	314, 422, 555, 1413
Csp6I	GTAC	6 cut(s)	20, 74, 182, 392, 1448, 1699
CviQI	GTAC	6 cut(s)	20, 74, 182, 392, 1448, 1699
DdeI	CTNAG	6 cut(s)	315, 989, 1056, 1296, 1503, 1675
DpnI	GATC	3 cut(s)	886, 1065, 1325
DpnII	GATC	3 cut(s)	884, 1063, 1323
EaeI	YGGCCR	1 cut(s)	485
Eam1104I	CTCTTC	3 cut(s)	333, 915, 1252
EarI	CTCTTC	3 cut(s)	333, 915, 1252
Ecl136II	GAGCTC	1 cut(s)	590
Eco130I	CCWWGG	1 cut(s)	1585
Eco24I	GRGCYC	1 cut(s)	592
Eco47I	GGWCC	4 cut(s)	611, 1091, 1130, 1582
Eco53kI	GAGCTC	1 cut(s)	590
Eco57I	CTGAAG	1 cut(s)	54
Eco88I	CYCGRG	1 cut(s)	616
EcoICRI	GAGCTC	1 cut(s)	590
EcoO109I	RGGNCCY	1 cut(s)	1130
EcoT14I	CCWWGG	1 cut(s)	1585
EcoT22I	ATGCAT	3 cut(s)	238, 1038, 1627
EcoT38I	GRGCYC	1 cut(s)	592
ErhI	CCWWGG	1 cut(s)	1585
FalI	AAGNNNNNCTT	2 cut(s)	258, 290
FaqI	GGGAC	1 cut(s)	597
FauI	CCCGC	1 cut(s)	448
FauNDI	CATATG	2 cut(s)	1038, 1354
Fnu4HI	GCNGC	9 cut(s)	260, 447, 785, 795, 981, 1145, 1178, 1500, 1556
FokI	GGATG	6 cut(s)	495, 520, 701, 743, 1092, 1373
FriOI	GRGCYC	1 cut(s)	592
Fsp4HI	GCNGC	9 cut(s)	260, 447, 785, 795, 981, 1145, 1178, 1500, 1556
FspBI	CTAG	4 cut(s)	149, 1410, 1440, 1702
FspI	TGCGCA	1 cut(s)	522
GlaI	GCGC	4 cut(s)	363, 408, 522, 1408
GluI	GCNGC	9 cut(s)	260, 447, 785, 795, 981, 1145, 1178, 1500, 1556
GsaI	CCCAGC	3 cut(s)	158, 683, 1548
HaeIII	GGCC	7 cut(s)	176, 350, 478, 487, 693, 1072, 1144
HapII	CCGG	5 cut(s)	161, 505, 617, 755, 1199
HgaI	GACGC	4 cut(s)	314, 422, 555, 1413
HhaI	GCGC	4 cut(s)	364, 409, 523, 1409
Hin6I	GCGC	4 cut(s)	362, 407, 521, 1407
HinP1I	GCGC	4 cut(s)	362, 407, 521, 1407
HincII	GTYRAC	1 cut(s)	1006
HindII	GTYRAC	1 cut(s)	1006
HindIII	AAGCTT	1 cut(s)	811
HinfI	GANTC	2 cut(s)	665, 737
HpaII	CCGG	5 cut(s)	161, 505, 617, 755, 1199
HphI	GGTGA	6 cut(s)	80, 113, 476, 746, 953, 1318
Hpy166II	GTNNAC	6 cut(s)	706, 958, 1006, 1094, 1448, 1582
Hpy188I	TCNGA	2 cut(s)	889, 1059
Hpy188III	TCNNGA	8 cut(s)	149, 161, 437, 505, 605, 851, 914, 1487
Hpy8I	GTNNAC	6 cut(s)	706, 958, 1006, 1094, 1448, 1582
Hpy99I	CGWCG	1 cut(s)	1407
HpyCH4III	ACNGT	6 cut(s)	85, 246, 282, 838, 1423, 1465
HpyF10VI	GCNNNNNNNGC	7 cut(s)	452, 484, 765, 803, 1301, 1345, 1415
HpyF3I	CTNAG	6 cut(s)	315, 989, 1056, 1296, 1503, 1675
HspAI	GCGC	4 cut(s)	362, 407, 521, 1407
Kpn2I	TCCGGA	2 cut(s)	160, 504
Kzo9I	GATC	3 cut(s)	884, 1063, 1323
LguI	GCTCTTC	1 cut(s)	915
LmnI	GCTCC	7 cut(s)	163, 163, 331, 658, 754, 1270, 1489
Lsp1109I	GCAGC	8 cut(s)	271, 433, 771, 806, 992, 1189, 1511, 1567
LweI	GCATC	8 cut(s)	223, 259, 433, 498, 866, 1351, 1531, 1634
MaeI	CTAG	4 cut(s)	149, 1410, 1440, 1702
MaeIII	GTNAC	4 cut(s)	79, 208, 1315, 1423
MalI	GATC	3 cut(s)	886, 1065, 1325
MboI	GATC	3 cut(s)	884, 1063, 1323
MfeI	CAATTG	2 cut(s)	966, 1334
MhlI	GDGCHC	4 cut(s)	592, 960, 1486, 1552
MlsI	TGGCCA	1 cut(s)	487
MluNI	TGGCCA	1 cut(s)	487
Mox20I	TGGCCA	1 cut(s)	487
Mph1103I	ATGCAT	3 cut(s)	238, 1038, 1627
MroI	TCCGGA	2 cut(s)	160, 504
MscI	TGGCCA	1 cut(s)	487
MseI	TTAA	3 cut(s)	564, 597, 840
MslI	CAYNNNNRTG	1 cut(s)	441
Msp20I	TGGCCA	1 cut(s)	487
MspA1I	CMGCKG	2 cut(s)	1348, 1379
MspI	CCGG	5 cut(s)	161, 505, 617, 755, 1199
MunI	CAATTG	2 cut(s)	966, 1334
Mva1269I	GAATGC	1 cut(s)	118
MvnI	CGCG	1 cut(s)	1407
MwoI	GCNNNNNNNGC	7 cut(s)	452, 484, 765, 803, 1301, 1345, 1415
NciI	CCSGG	3 cut(s)	617, 618, 756
NdeI	CATATG	2 cut(s)	1038, 1354
NdeII	GATC	3 cut(s)	884, 1063, 1323
NlaIV	GGNNCC	7 cut(s)	516, 613, 660, 1092, 1131, 1276, 1551
NmeAIII	GCCGAG	1 cut(s)	1153
NmuCI	GTSAC	3 cut(s)	79, 208, 1315
NsbI	TGCGCA	1 cut(s)	522
NsiI	ATGCAT	3 cut(s)	238, 1038, 1627
NspI	RCATGY	3 cut(s)	1165, 1210, 1652
PagI	TCATGA	1 cut(s)	436
PciSI	GCTCTTC	1 cut(s)	915
PctI	GAATGC	1 cut(s)	118
PfeI	GAWTC	2 cut(s)	665, 737
PflMI	CCANNNNNTGG	1 cut(s)	1571
PfoI	TCCNGGA	1 cut(s)	1132
PkrI	GCNGC	9 cut(s)	261, 448, 786, 796, 982, 1146, 1179, 1501, 1557
PpuMI	RGGWCCY	1 cut(s)	1130
PsiI	TTATAA	1 cut(s)	1593
Psp124BI	GAGCTC	1 cut(s)	592
Psp5II	RGGWCCY	1 cut(s)	1130
PspFI	CCCAGC	3 cut(s)	154, 679, 1544
PspN4I	GGNNCC	7 cut(s)	516, 613, 660, 1092, 1131, 1276, 1551
PspPI	GGNCC	7 cut(s)	349, 611, 1070, 1091, 1130, 1142, 1582
PspPPI	RGGWCCY	1 cut(s)	1130
PstNI	CAGNNNCTG	2 cut(s)	124, 1510
PvuII	CAGCTG	1 cut(s)	1379
RsaI	GTAC	6 cut(s)	21, 75, 183, 393, 1449, 1700
RsaNI	GTAC	6 cut(s)	20, 74, 182, 392, 1448, 1699
RseI	CAYNNNNRTG	1 cut(s)	441
SacI	GAGCTC	1 cut(s)	592
SapI	GCTCTTC	1 cut(s)	915
SaqAI	TTAA	3 cut(s)	564, 597, 840
SatI	GCNGC	9 cut(s)	260, 447, 785, 795, 981, 1145, 1178, 1500, 1556
Sau3AI	GATC	3 cut(s)	884, 1063, 1323
Sau96I	GGNCC	7 cut(s)	349, 611, 1070, 1091, 1130, 1142, 1582
SduI	GDGCHC	4 cut(s)	592, 960, 1486, 1552
SfaNI	GCATC	8 cut(s)	223, 259, 433, 498, 866, 1351, 1531, 1634
SfiI	GGCCNNNNNGGCC	1 cut(s)	484
SinI	GGWCC	4 cut(s)	611, 1091, 1130, 1582
SmaI	CCCGGG	1 cut(s)	618
SmiMI	CAYNNNNRTG	1 cut(s)	441
SmlI	CTYRAG	1 cut(s)	1098
SmoI	CTYRAG	1 cut(s)	1098
SsiI	CCGC	7 cut(s)	455, 1009, 1145, 1302, 1346, 1358, 1553
SspI	AATATT	1 cut(s)	1026
SspMI	CTAG	4 cut(s)	149, 1410, 1440, 1702
SstI	GAGCTC	1 cut(s)	592
StyI	CCWWGG	1 cut(s)	1585
TaaI	ACNGT	6 cut(s)	85, 246, 282, 838, 1423, 1465
TaqI	TCGA	2 cut(s)	96, 1402
TatI	WGTACW	2 cut(s)	181, 391
TauI	GCSGC	1 cut(s)	1147
TfiI	GAWTC	2 cut(s)	665, 737
Tru1I	TTAA	3 cut(s)	564, 597, 840
Tru9I	TTAA	3 cut(s)	564, 597, 840
TscAI	CASTG	4 cut(s)	129, 315, 351, 1480
TseFI	GTSAC	3 cut(s)	79, 208, 1315
TseI	GCWGC	8 cut(s)	259, 446, 784, 794, 980, 1177, 1499, 1555
Tsp45I	GTSAC	3 cut(s)	79, 208, 1315
TspDTI	ATGAA	4 cut(s)	207, 453, 550, 1522
TspGWI	ACGGA	1 cut(s)	185
TspMI	CCCGGG	1 cut(s)	616
TspRI	CASTG	4 cut(s)	129, 315, 351, 1480
Van91I	CCANNNNNTGG	1 cut(s)	1571
VneI	GTGCAC	1 cut(s)	956
VpaK11BI	GGWCC	4 cut(s)	611, 1091, 1130, 1582
XapI	RAATTY	2 cut(s)	600, 1341
XbaI	TCTAGA	1 cut(s)	148
XceI	RCATGY	3 cut(s)	1165, 1210, 1652
XcmI	CCANNNNNNNNNTGG	1 cut(s)	784
XmaI	CCCGGG	1 cut(s)	616
XspI	CTAG	4 cut(s)	149, 1410, 1440, 1702
Zsp2I	ATGCAT	3 cut(s)	238, 1038, 1627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.