Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007921

Physical Location & Seq

Genomic Coordinates Forward (+)

31456231 .. 31461644

Gene Structure 5414 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1244000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGGGTTTCGATTCGGAGTATTTTCTTCATTTGGGTTTACAATTTATGGCTGAAGCTGATAAACTCTATGGGGAACTTGATGGAGGTGAAGTACCTGTGACCGTGGCTGAAATCAATGCCGAGATTGAGAATGCAGAGCCGCTGGTAGATGACCAGGCTACGGCACTAGACCCGGCTTCGGAGCTGACAGAGGCCGATGTACAGGGGTATGAACAAGAGCATGGAGTGACATTTGCACAAGAACAAGAAGATGCACTTGCCCAGTTCCGGGCACAGGAGCAGGAAGAGGCACTGGCCCAGTTCCAGGCACAGGAGCAGGAAGAGGCACTGGCCCAGTTCCAGGCACAGGAGCAAGAAGAGGCACTGGCCCAGTTCCAGGCGCAGGAACAAGTAGAGTTATCGGTAGAAGTGCAAGAGGAGGAGGCGCAGTTATCCGAACAGAACCCAGAGCGTCACGAAGATGCTGCTGCCTTTGGTGGAGGTGAGAAGAAGTGGCCTGGATGGCCCGGAGAGAGTGTTTTCCGGATGCTGGTTCCTGCGCAGAAGGTTGGCAGTATAATTGGACGGAAAGGGGAGTTCATTAAGAAAATAGTTGAGGAGACGAGAGCTCGCATTAAAATTCTTGATGGACCCCCAGGAACGACTGAAAGAGCCGTAATGGTTTCTGGTAAGGAGGAGCCTGATTCTTCTCTTCCCCCTGCAATGGATGGCCTTTTGAGGGTTCACACGCGTATTATTGATGGTTTGGATGGTGATTCATCTCATGCTCCACCGGGCATGGGTGGCAAAGTCTCTACAAAGCTGCTGGTTGCAGCTTCACAGGCAGGAAGCTTGATTGGGAAACAGGGAGGAACTGTTAAATCCATTCAAGAAGCATCTAACTGCATAGTTAGAGTTCTTGGATCAGAAGACCTACCGATTTTTGCTCTTCAAGATGACAGAGTGGTTGAAGTAGTGGGTGAAGCTGTTGGTGTGCACAAAGCAATTGAACTAATTGCATCTCATCTTAGGAAGTTTTTAGTTGACCGCAGTATAATTCCAATATTTGAAATGCATATGCAAATGGCAAATCCTCAGATGGAACATGGGCCACCTCATCAAAATTGGGGTCCACCTCAAGGTCATCCCCACAATGCTGGTCATGGGGGTCCTGGATTTGGGCCGCCTAATCCCCAATACATGCCACCTCCTCGGCAACATGACAATTACTATCCACCAGCTGACATGCCTCCACCAATGGAGAAACAGCCTCACCATGGTATATCGGCTTATGGAAGAGAAGCTCCAATGGGTGTCCACCAGTCATCAAATACTCAGTCCGCACCAGGAATGGTAACACAGATCACCAAGCAATTGCAAATTCCGCTGTCATATGCTGATGCTGTTATTGGCACAGCTGGTGCAAGTATAAGCTATATTCGACGAGCTAGTGGGGCAACTGTTACGATACAAGAGACTAGGGGTGTTCCTGGGGAGATGACTGTTGAAATCAGTGGAAGTGCTCCCGAAGTTCAAGCAGCTCAACAACTGATACAGAATTTCATGGCAGATGCTGGAGCACCGCAGCCGACACCAACTCCTGGCTCTGTGGACCAAGGTTATAACAACTATGCCACTCACGGTTCAGTTTATGCATCTCCCCCATCTAATCAAGGACACGCAGGACAACACGGCGGCTATGGCTCAGTCTATGGCTCGCATTATGGGTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009909 GO:0009911 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0097159 GO:1901363 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

571

Amino Acids

61.24

Weight (kDa)

4.74

Isoelectric Point (pI)

47.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	174 - 226	1.6e-14	KH domain
KH_1	PF00013	266 - 333	4.6e-14	KH domain
KH_1	PF00013	449 - 512	6.5e-12	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04610 AT3G04610 AT3G04610 AT3G04610
fragaria_vesca	FvH4_6g02580 FvH4_6g02580 FvH4_6g02580 FvH4_6g02580
malus_domestica	MD04G1227000.v1.1 MD12G1244000.v1.1
prunus_persica	Prupe.6G345700_v2.0.a1 Prupe.6G345700_v2.0.a1
pyrus_communis	pycom04g20030 pycom12g22300
rosa_chinensis	RchiOBHm_Chr3g0450491
rosa_laevigata	RLG00000025727
rosa_multiflora	Rmu_sc0005951.1_g000006
rosa_roxburghii	Rroxscaffold_6G00428170
rosa_rugosa	Rorug02G0628000
rosa_samantha	Rh3AG028700 Rh3BG029200 Rh3CG028000 Rh3DG028900
rosa_wichuraiana	Rw3G002210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1602
Acc16I	TGCGCA	1 cut(s)	540
AccB7I	CCANNNNNTGG	1 cut(s)	1580
AccII	CGCG	1 cut(s)	730
AccIII	TCCGGA	1 cut(s)	522
AciI	CCGC	7 cut(s)	140, 1027, 1163, 1320, 1364, 1562, 1674
AclWI	GGATC	1 cut(s)	910
AcsI	RAATTY	3 cut(s)	618, 1359, 1537
AcuI	CTGAAG	1 cut(s)	72
AfaI	GTAC	3 cut(s)	93, 201, 1709
AflIII	ACRYGT	1 cut(s)	728
AgsI	TTSAA	7 cut(s)	869, 932, 950, 989, 1049, 1487, 1514
Alw21I	GWGCWC	4 cut(s)	610, 978, 1504, 1561
Alw26I	GTCTC	3 cut(s)	593, 796, 1448
Alw44I	GTGCAC	1 cut(s)	974
AlwI	GGATC	1 cut(s)	910
AlwNI	CAGNNNCTG	1 cut(s)	1553
Aor13HI	TCCGGA	1 cut(s)	522
AoxI	GGCC	9 cut(s)	192, 294, 330, 366, 494, 503, 709, 1088, 1160
ApaLI	GTGCAC	1 cut(s)	974
ApeKI	GCWGC	6 cut(s)	464, 467, 802, 812, 1517, 1564
ApoI	RAATTY	3 cut(s)	618, 1359, 1537
ArsI	GACNNNNNNTTYG	4 cut(s)	1093, 1125, 1301, 1333
AspLEI	GCGC	3 cut(s)	382, 427, 541
AsuC2I	CCSGG	4 cut(s)	173, 269, 507, 774
AsuHPI	GGTGA	6 cut(s)	98, 494, 764, 971, 1244, 1336
AvaII	GGWCC	4 cut(s)	629, 1109, 1148, 1591
BaeGI	GKGCMC	2 cut(s)	274, 978
BanII	GRGCYC	1 cut(s)	610
BbsI	GAAGAC	1 cut(s)	915
Bbv12I	GWGCWC	4 cut(s)	610, 978, 1504, 1561
BbvI	GCAGC	6 cut(s)	451, 454, 789, 824, 1529, 1576
BccI	CCATC	8 cut(s)	74, 495, 620, 701, 734, 743, 1072, 1651
BceAI	ACGGC	3 cut(s)	177, 638, 1687
BcgI	CGANNNNNNTGC	2 cut(s)	446, 480
BcnI	CCSGG	4 cut(s)	173, 269, 507, 774
BcoDI	GTCTC	3 cut(s)	593, 796, 1448
BfaI	CTAG	4 cut(s)	167, 1428, 1458, 1711
BglI	GCCNNNNNGGC	1 cut(s)	502
BisI	GCNGC	9 cut(s)	140, 465, 468, 803, 813, 1163, 1518, 1565, 1675
BlsI	GCNGC	9 cut(s)	141, 466, 469, 804, 814, 1164, 1519, 1566, 1676
Bme18I	GGWCC	4 cut(s)	629, 1109, 1148, 1591
BmiI	GGNNCC	5 cut(s)	534, 631, 678, 1110, 1149
BmrI	ACTGGG	4 cut(s)	256, 292, 328, 364
BmsI	GCATC	8 cut(s)	241, 451, 516, 884, 1007, 1369, 1540, 1643
BmuI	ACTGGG	4 cut(s)	256, 292, 328, 364
BpiI	GAAGAC	1 cut(s)	915
BpmI	CTGGAG	1 cut(s)	1575
BpuEI	CTTGAG	1 cut(s)	1101
BpuMI	CCSGG	4 cut(s)	173, 269, 507, 774
BsaJI	CCNNGG	6 cut(s)	102, 634, 1190, 1255, 1469, 1594
BsaWI	WCCGGW	1 cut(s)	522
Bse1I	ACTGG	8 cut(s)	262, 297, 298, 333, 334, 369, 370, 1300
Bse3DI	GCAATG	1 cut(s)	708
BseAI	TCCGGA	1 cut(s)	522
BseDI	CCNNGG	6 cut(s)	102, 634, 1190, 1255, 1469, 1594
BseGI	GGATG	5 cut(s)	506, 531, 712, 754, 1123
BseMI	GCAATG	1 cut(s)	708
BseMII	CTCAG	3 cut(s)	1088, 1328, 1698
BseNI	ACTGG	8 cut(s)	262, 297, 298, 333, 334, 369, 370, 1300
BseRI	GAGGAG	5 cut(s)	431, 434, 611, 689, 1179
BseSI	GKGCMC	2 cut(s)	274, 978
BseXI	GCAGC	6 cut(s)	451, 454, 789, 824, 1529, 1576
Bsh1236I	CGCG	1 cut(s)	730
BshFI	GGCC	9 cut(s)	194, 296, 332, 368, 496, 505, 711, 1090, 1162
BsiHKAI	GWGCWC	4 cut(s)	610, 978, 1504, 1561
BsiSI	CCGG	5 cut(s)	173, 268, 507, 523, 773
BsmAI	GTCTC	3 cut(s)	593, 796, 1448
BsmBI	CGTCTC	1 cut(s)	593
BsmI	GAATGC	1 cut(s)	136
BsnI	GGCC	9 cut(s)	194, 296, 332, 368, 496, 505, 711, 1090, 1162
Bsp1286I	GDGCHC	5 cut(s)	274, 610, 978, 1504, 1561
Bsp13I	TCCGGA	1 cut(s)	522
Bsp1407I	TGTACA	1 cut(s)	199
Bsp143I	GATC	2 cut(s)	902, 1341
Bsp19I	CCATGG	1 cut(s)	1255
BspACI	CCGC	7 cut(s)	140, 1027, 1163, 1320, 1364, 1562, 1674
BspANI	GGCC	9 cut(s)	194, 296, 332, 368, 496, 505, 711, 1090, 1162
BspCNI	CTCAG	3 cut(s)	1087, 1327, 1697
BspEI	TCCGGA	1 cut(s)	522
BspFNI	CGCG	1 cut(s)	730
BspLI	GGNNCC	5 cut(s)	534, 631, 678, 1110, 1149
BspPI	GGATC	1 cut(s)	910
BspQI	GCTCTTC	1 cut(s)	933
BsrDI	GCAATG	1 cut(s)	708
BsrGI	TGTACA	1 cut(s)	199
BsrI	ACTGG	8 cut(s)	262, 297, 298, 333, 334, 369, 370, 1300
BssECI	CCNNGG	6 cut(s)	102, 634, 1190, 1255, 1469, 1594
BssMI	GATC	2 cut(s)	902, 1341
BssT1I	CCWWGG	2 cut(s)	1255, 1594
Bst4CI	ACNGT	5 cut(s)	103, 856, 1441, 1483, 1622
Bst6I	CTCTTC	6 cut(s)	279, 315, 351, 696, 933, 1270
BstAUI	TGTACA	1 cut(s)	199
BstC8I	GCNNGC	2 cut(s)	610, 1697
BstDEI	CTNAG	4 cut(s)	1007, 1074, 1314, 1684
BstDSI	CCRYGG	2 cut(s)	102, 1255
BstF5I	GGATG	5 cut(s)	506, 531, 712, 754, 1123
BstFNI	CGCG	1 cut(s)	730
BstHHI	GCGC	3 cut(s)	382, 427, 541
BstKTI	GATC	2 cut(s)	905, 1344
BstMAI	GTCTC	3 cut(s)	593, 796, 1448
BstMBI	GATC	2 cut(s)	902, 1341
BstMWI	GCNNNNNNNGC	6 cut(s)	502, 783, 821, 1363, 1433, 1680
BstNSI	RCATGY	2 cut(s)	1183, 1228
BstSLI	GKGCMC	2 cut(s)	274, 978
BstUI	CGCG	1 cut(s)	730
BstV1I	GCAGC	6 cut(s)	451, 454, 789, 824, 1529, 1576
BstV2I	GAAGAC	1 cut(s)	915
BstXI	CCANNNNNNTGG	1 cut(s)	1136
BsuRI	GGCC	9 cut(s)	194, 296, 332, 368, 496, 505, 711, 1090, 1162
BtgI	CCRYGG	2 cut(s)	102, 1255
BtsCI	GGATG	5 cut(s)	506, 531, 712, 754, 1123
BtsIMutI	CAGTG	4 cut(s)	290, 326, 362, 1498
Cac8I	GCNNGC	2 cut(s)	610, 1697
CaiI	CAGNNNCTG	1 cut(s)	1553
CfoI	GCGC	3 cut(s)	382, 427, 541
CseI	GACGC	1 cut(s)	440
Csp6I	GTAC	3 cut(s)	92, 200, 1708
CviQI	GTAC	3 cut(s)	92, 200, 1708
DdeI	CTNAG	4 cut(s)	1007, 1074, 1314, 1684
DpnI	GATC	2 cut(s)	904, 1343
DpnII	GATC	2 cut(s)	902, 1341
Eam1104I	CTCTTC	6 cut(s)	279, 315, 351, 696, 933, 1270
EarI	CTCTTC	6 cut(s)	279, 315, 351, 696, 933, 1270
Ecl136II	GAGCTC	1 cut(s)	608
Eco130I	CCWWGG	2 cut(s)	1255, 1594
Eco24I	GRGCYC	1 cut(s)	610
Eco47I	GGWCC	4 cut(s)	629, 1109, 1148, 1591
Eco53kI	GAGCTC	1 cut(s)	608
Eco57I	CTGAAG	1 cut(s)	72
EcoICRI	GAGCTC	1 cut(s)	608
EcoO109I	RGGNCCY	1 cut(s)	1148
EcoT14I	CCWWGG	2 cut(s)	1255, 1594
EcoT22I	ATGCAT	2 cut(s)	1056, 1636
EcoT38I	GRGCYC	1 cut(s)	610
ErhI	CCWWGG	2 cut(s)	1255, 1594
Esp3I	CGTCTC	1 cut(s)	593
FalI	AAGNNNNNCTT	2 cut(s)	240, 272
FauNDI	CATATG	2 cut(s)	1056, 1372
Fnu4HI	GCNGC	9 cut(s)	140, 465, 468, 803, 813, 1163, 1518, 1565, 1675
FokI	GGATG	5 cut(s)	513, 538, 719, 761, 1110
FriOI	GRGCYC	1 cut(s)	610
Fsp4HI	GCNGC	9 cut(s)	140, 465, 468, 803, 813, 1163, 1518, 1565, 1675
FspBI	CTAG	4 cut(s)	167, 1428, 1458, 1711
FspI	TGCGCA	1 cut(s)	540
GlaI	GCGC	3 cut(s)	381, 426, 540
GluI	GCNGC	9 cut(s)	140, 465, 468, 803, 813, 1163, 1518, 1565, 1675
GsuI	CTGGAG	1 cut(s)	1575
HaeIII	GGCC	9 cut(s)	194, 296, 332, 368, 496, 505, 711, 1090, 1162
HapII	CCGG	5 cut(s)	173, 268, 507, 523, 773
HgaI	GACGC	1 cut(s)	440
HhaI	GCGC	3 cut(s)	382, 427, 541
Hin6I	GCGC	3 cut(s)	380, 425, 539
HinP1I	GCGC	3 cut(s)	380, 425, 539
HincII	GTYRAC	1 cut(s)	1024
HindII	GTYRAC	1 cut(s)	1024
HindIII	AAGCTT	1 cut(s)	829
HinfI	GANTC	3 cut(s)	11, 683, 755
HpaII	CCGG	5 cut(s)	173, 268, 507, 523, 773
HphI	GGTGA	6 cut(s)	98, 494, 764, 971, 1244, 1336
Hpy166II	GTNNAC	7 cut(s)	38, 724, 976, 1024, 1112, 1297, 1591
Hpy188I	TCNGA	5 cut(s)	16, 181, 436, 907, 1077
Hpy188III	TCNNGA	6 cut(s)	455, 523, 623, 869, 932, 1505
Hpy8I	GTNNAC	7 cut(s)	38, 724, 976, 1024, 1112, 1297, 1591
Hpy99I	CGWCG	1 cut(s)	1425
HpyAV	CCTTC	1 cut(s)	538
HpyCH4III	ACNGT	5 cut(s)	103, 856, 1441, 1483, 1622
HpyF10VI	GCNNNNNNNGC	6 cut(s)	502, 783, 821, 1363, 1433, 1680
HpyF3I	CTNAG	4 cut(s)	1007, 1074, 1314, 1684
HspAI	GCGC	3 cut(s)	380, 425, 539
Kpn2I	TCCGGA	1 cut(s)	522
Kzo9I	GATC	2 cut(s)	902, 1341
LguI	GCTCTTC	1 cut(s)	933
LmnI	GCTCC	9 cut(s)	181, 277, 313, 349, 676, 772, 1288, 1507, 1556
Lsp1109I	GCAGC	6 cut(s)	451, 454, 789, 824, 1529, 1576
LweI	GCATC	8 cut(s)	241, 451, 516, 884, 1007, 1369, 1540, 1643
MaeI	CTAG	4 cut(s)	167, 1428, 1458, 1711
MaeIII	GTNAC	5 cut(s)	97, 226, 452, 1333, 1441
MalI	GATC	2 cut(s)	904, 1343
MboI	GATC	2 cut(s)	902, 1341
MfeI	CAATTG	2 cut(s)	984, 1352
MhlI	GDGCHC	5 cut(s)	274, 610, 978, 1504, 1561
MluI	ACGCGT	1 cut(s)	728
Mph1103I	ATGCAT	2 cut(s)	1056, 1636
MroI	TCCGGA	1 cut(s)	522
MseI	TTAA	3 cut(s)	582, 615, 858
MslI	CAYNNNNRTG	1 cut(s)	459
MspA1I	CMGCKG	4 cut(s)	142, 1220, 1366, 1397
MspI	CCGG	5 cut(s)	173, 268, 507, 523, 773
MunI	CAATTG	2 cut(s)	984, 1352
Mva1269I	GAATGC	1 cut(s)	136
MvnI	CGCG	1 cut(s)	730
MwoI	GCNNNNNNNGC	6 cut(s)	502, 783, 821, 1363, 1433, 1680
NciI	CCSGG	4 cut(s)	173, 269, 507, 774
NcoI	CCATGG	1 cut(s)	1255
NdeI	CATATG	2 cut(s)	1056, 1372
NdeII	GATC	2 cut(s)	902, 1341
NlaIV	GGNNCC	5 cut(s)	534, 631, 678, 1110, 1149
NmeAIII	GCCGAG	2 cut(s)	145, 1171
NmuCI	GTSAC	3 cut(s)	97, 226, 452
NsbI	TGCGCA	1 cut(s)	540
NsiI	ATGCAT	2 cut(s)	1056, 1636
NspI	RCATGY	2 cut(s)	1183, 1228
PciSI	GCTCTTC	1 cut(s)	933
PctI	GAATGC	1 cut(s)	136
PfeI	GAWTC	3 cut(s)	11, 683, 755
PflMI	CCANNNNNTGG	1 cut(s)	1580
PfoI	TCCNGGA	1 cut(s)	1150
PkrI	GCNGC	9 cut(s)	141, 466, 469, 804, 814, 1164, 1519, 1566, 1676
PpuMI	RGGWCCY	1 cut(s)	1148
PsiI	TTATAA	1 cut(s)	1602
Psp124BI	GAGCTC	1 cut(s)	610
Psp5II	RGGWCCY	1 cut(s)	1148
PspN4I	GGNNCC	5 cut(s)	534, 631, 678, 1110, 1149
PspPPI	RGGWCCY	1 cut(s)	1148
PstNI	CAGNNNCTG	1 cut(s)	1553
PvuII	CAGCTG	2 cut(s)	1220, 1397
RsaI	GTAC	3 cut(s)	93, 201, 1709
RsaNI	GTAC	3 cut(s)	92, 200, 1708
RseI	CAYNNNNRTG	1 cut(s)	459
SacI	GAGCTC	1 cut(s)	610
SapI	GCTCTTC	1 cut(s)	933
SaqAI	TTAA	3 cut(s)	582, 615, 858
SatI	GCNGC	9 cut(s)	140, 465, 468, 803, 813, 1163, 1518, 1565, 1675
Sau3AI	GATC	2 cut(s)	902, 1341
SduI	GDGCHC	5 cut(s)	274, 610, 978, 1504, 1561
SfaNI	GCATC	8 cut(s)	241, 451, 516, 884, 1007, 1369, 1540, 1643
SfiI	GGCCNNNNNGGCC	1 cut(s)	502
SinI	GGWCC	4 cut(s)	629, 1109, 1148, 1591
SmiMI	CAYNNNNRTG	1 cut(s)	459
SmlI	CTYRAG	1 cut(s)	1116
SmoI	CTYRAG	1 cut(s)	1116
SsiI	CCGC	7 cut(s)	140, 1027, 1163, 1320, 1364, 1562, 1674
SspI	AATATT	1 cut(s)	1044
SspMI	CTAG	4 cut(s)	167, 1428, 1458, 1711
SstI	GAGCTC	1 cut(s)	610
StyI	CCWWGG	2 cut(s)	1255, 1594
TaaI	ACNGT	5 cut(s)	103, 856, 1441, 1483, 1622
TaqI	TCGA	2 cut(s)	9, 1420
TatI	WGTACW	1 cut(s)	199
TauI	GCSGC	3 cut(s)	142, 1165, 1677
TfiI	GAWTC	3 cut(s)	11, 683, 755
Tru1I	TTAA	3 cut(s)	582, 615, 858
Tru9I	TTAA	3 cut(s)	582, 615, 858
TscAI	CASTG	4 cut(s)	297, 333, 369, 1498
TseFI	GTSAC	3 cut(s)	97, 226, 452
TseI	GCWGC	6 cut(s)	464, 467, 802, 812, 1517, 1564
Tsp45I	GTSAC	3 cut(s)	97, 226, 452
TspDTI	ATGAA	5 cut(s)	17, 225, 568, 747, 1531
TspGWI	ACGGA	1 cut(s)	580
TspRI	CASTG	4 cut(s)	297, 333, 369, 1498
Van91I	CCANNNNNTGG	1 cut(s)	1580
VneI	GTGCAC	1 cut(s)	974
VpaK11BI	GGWCC	4 cut(s)	629, 1109, 1148, 1591
XapI	RAATTY	3 cut(s)	618, 1359, 1537
XceI	RCATGY	2 cut(s)	1183, 1228
XspI	CTAG	4 cut(s)	167, 1428, 1458, 1711
Zsp2I	ATGCAT	2 cut(s)	1056, 1636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.