MD04G1229100.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010655
Physical Location & Seq
Forward (+)
30925518 .. 30929906
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1229100.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGACCAATCGGTTCTGGACGACATAATCAACCGACTCCTCGAAGTCCGAGGCCGGCCCGGGAAGCAGGTCCAGCTGTCGGAGTCGGAGATCCGGCAGCTTTGTTTGGTCTCTAAAGAAATCTTCTTGCAGCAGCCTAATTTGTTAGAGCTCGAAGCACCCATCAAGATTTGTGGTGATATACATGGTCAGTACTCGGATCTTTTAAGGCTTTTCGAGTACGGTGGACTGCCTCCTAATGCCAATTACTTGTTCTTGGGGGATTATGTGGATCGGGGAAAGCAAAGTTTAGAAACAATATGTCTTCTCCTTGCATATAAAATAAAATATCCTGAGAATTTTTTCCTCTTGAGGGGAAACCATGAATGTGCATCTATAAACCGTATATACGGATTTTATGATGAGTGTAAGAGAAGATTCAATGTGAGGCTATGGAAGACATTCACAGAGTGTTTTAACTGCCTTCCAGTGGCAGCTCTGATTGATGAAAAGATTCTCTGCATGCATGGTGGTCTTTCTCCCGACCTGCATAATTTAGATCAAATCAGAAATTTACAGCGGCCAACAGATGTACCAGACACAGGTTTACTTTGTGACCTTCTCTGGTCTGATCCCAGTAAAGATGTTCATGGTTGGGGAGGGAACGAGCGGGGAGTTTCGTTTACATTTGGTCCTGACACGGTGACGGAGTTTCTTCAGAAGCATGATTTGGATCTCATTTGTCGTGCTCACCAGGTTGTGGAGGATGGCTACGAGTTATTTGCCAACCGACAACTTGTGACGATATTTTCAGCACCTAATTATTGTGGGGAATTTGATAATGCTGGTGCCATGATGAGTGTGGATGAGACATTAATGTGCTCTTTTCAAATATTAAAACCCGCAGAGAAAAAGCCGAAATTTAACTTTGGGAGCATGACTGCAGCTAAGCCTGGAAATACTTCATCTGGAGTTGGTGCTTTTGCGAGCATAGCAGCAGCTAAGCCTGGGAACACTGTTACAGGAATCAAGTCTCTGATGCACAGGTAA

Protein Analysis

343

Amino Acids

38.38

Weight (kDa)

5.67

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STPPase_N PF16891 6 - 52 1.4e-22 Serine-threonine protein phosphatase N-terminal domain
Metallophos PF00149 54 - 247 6.4e-53 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010655)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29400 AT3G46820 AT5G59160 AT5G59160 AT5G59160
fragaria_vesca FvH4_6g02160 FvH4_6g02160
malus_domestica MD04G1229100.v1.1 MD12G1246200.v1.1
prunus_persica Prupe.6G349000_v2.0.a1
pyrus_communis pycom04g20300 pycom12g22570
rosa_chinensis RchiOBHm_Chr3g0449941
rosa_laevigata RLG00000025773
rosa_multiflora Rmu_sc0002646.1_g000002
rosa_roxburghii Rroxscaffold_6G00427730
rosa_rugosa Rorug02G0624100
rosa_samantha Rh3AG023200 Rh3DG023700
rosa_wichuraiana Rw3G001860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 58, 534
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 739
AccBSI CCGCTC 1 cut(s) 649
AciI CCGC 3 cut(s) 559, 649, 882
AclWI GGATC 5 cut(s) 85, 207, 279, 605, 720
AcoI YGGCCR 1 cut(s) 560
AcsI RAATTY 4 cut(s) 337, 550, 812, 899
AcuI CTGAAG 1 cut(s) 680
AdeI CACNNNGTG 1 cut(s) 450
AfaI GTAC 3 cut(s) 194, 221, 573
AfiI CCNNNNNNNGG 5 cut(s) 79, 352, 469, 581, 739
AgsI TTSAA 2 cut(s) 421, 869
AjnI CCWGG 3 cut(s) 732, 931, 985
AjuI GAANNNNNNNTTGG 4 cut(s) 236, 268, 692, 724
AluBI AGCT 6 cut(s) 76, 100, 151, 476, 926, 980
AluI AGCT 6 cut(s) 76, 100, 151, 476, 926, 980
Alw21I GWGCWC 3 cut(s) 153, 730, 863
Alw26I GTCTC 3 cut(s) 115, 842, 1017
AlwI GGATC 5 cut(s) 85, 207, 279, 605, 720
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 3 cut(s) 52, 56, 560
ApeKI GCWGC 7 cut(s) 97, 130, 133, 473, 923, 974, 977
ApoI RAATTY 4 cut(s) 337, 550, 812, 899
AseI ATTAAT 1 cut(s) 854
Asp700I GAANNNNTTC 4 cut(s) 341, 440, 492, 940
AspS9I GGNCC 4 cut(s) 4, 57, 70, 671
AsuC2I CCSGG 2 cut(s) 60, 61
AsuHPI GGTGA 3 cut(s) 188, 694, 722
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 3 cut(s) 4, 70, 671
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 153
BbsI GAAGAC 2 cut(s) 296, 443
Bbv12I GWGCWC 3 cut(s) 153, 730, 863
BbvI GCAGC 7 cut(s) 109, 142, 145, 485, 935, 986, 989
BccI CCATC 2 cut(s) 170, 740
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 3 cut(s) 734, 933, 987
BcnI CCSGG 2 cut(s) 60, 61
BcoDI GTCTC 3 cut(s) 115, 842, 1017
BfmI CTRYAG 1 cut(s) 921
BfuAI ACCTGC 2 cut(s) 58, 534
BisI GCNGC 8 cut(s) 98, 131, 134, 474, 560, 924, 975, 978
BlpI GCTNAGC 2 cut(s) 927, 981
BlsI GCNGC 8 cut(s) 99, 132, 135, 475, 561, 925, 976, 979
BmcAI AGTACT 1 cut(s) 194
Bme1390I CCNGG 5 cut(s) 60, 61, 734, 933, 987
Bme18I GGWCC 3 cut(s) 4, 70, 671
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 4 cut(s) 4, 57, 70, 671
BmiI GGNNCC 1 cut(s) 829
BmrFI CCNGG 5 cut(s) 60, 61, 734, 933, 987
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 380, 1008
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 2 cut(s) 296, 443
BpmI CTGGAG 1 cut(s) 969
Bpu1102I GCTNAGC 2 cut(s) 927, 981
BpuEI CTTGAG 1 cut(s) 370
BpuMI CCSGG 2 cut(s) 60, 61
BsaBI GATNNNNATC 1 cut(s) 711
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 3 cut(s) 49, 59, 986
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 352, 469, 581, 739
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 2 cut(s) 467, 615
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 3 cut(s) 734, 933, 987
BseDI CCNNGG 3 cut(s) 49, 59, 986
BseGI GGATG 2 cut(s) 751, 850
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 5 cut(s) 79, 352, 469, 581, 739
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 467, 615
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 7 cut(s) 109, 142, 145, 485, 935, 986, 989
BshFI GGCC 3 cut(s) 54, 58, 562
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 3 cut(s) 153, 730, 863
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 3 cut(s) 55, 60, 94
BslI CCNNNNNNNGG 5 cut(s) 79, 352, 469, 581, 739
BsmAI GTCTC 3 cut(s) 115, 842, 1017
BsnI GGCC 3 cut(s) 54, 58, 562
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 153, 730, 863
Bsp143I GATC 6 cut(s) 90, 199, 271, 538, 610, 712
Bsp1720I GCTNAGC 2 cut(s) 927, 981
BspACI CCGC 3 cut(s) 559, 649, 882
BspANI GGCC 3 cut(s) 54, 58, 562
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 829
BspMAI CTGCAG 1 cut(s) 925
BspMI ACCTGC 2 cut(s) 58, 534
BspPI GGATC 5 cut(s) 85, 207, 279, 605, 720
BspT107I GGYRCC 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 115
BsrBI CCGCTC 1 cut(s) 649
BsrFI RCCGGY 1 cut(s) 54
BsrI ACTGG 2 cut(s) 467, 615
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 3 cut(s) 49, 59, 986
BssMI GATC 6 cut(s) 90, 199, 271, 538, 610, 712
Bst2UI CCWGG 3 cut(s) 734, 933, 987
Bst4CI ACNGT 4 cut(s) 224, 383, 682, 997
BstC8I GCNNGC 3 cut(s) 56, 503, 967
BstDEI CTNAG 3 cut(s) 333, 927, 981
BstF5I GGATG 2 cut(s) 751, 850
BstKTI GATC 6 cut(s) 93, 202, 274, 541, 613, 715
BstMAI GTCTC 3 cut(s) 115, 842, 1017
BstMBI GATC 6 cut(s) 90, 199, 271, 538, 610, 712
BstMWI GCNNNNNNNGC 3 cut(s) 64, 73, 971
BstNI CCWGG 3 cut(s) 734, 933, 987
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 5 cut(s) 58, 59, 732, 931, 985
BstSFI CTRYAG 1 cut(s) 921
BstV1I GCAGC 7 cut(s) 109, 142, 145, 485, 935, 986, 989
BstV2I GAAGAC 2 cut(s) 296, 443
BstX2I RGATCY 3 cut(s) 90, 199, 712
BstYI RGATCY 3 cut(s) 90, 199, 712
BsuRI GGCC 3 cut(s) 54, 58, 562
BtsCI GGATG 2 cut(s) 751, 850
BtsIMutI CAGTG 2 cut(s) 474, 993
BveI ACCTGC 2 cut(s) 58, 534
Cac8I GCNNGC 3 cut(s) 56, 503, 967
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 4 cut(s) 4, 57, 70, 671
Cfr9I CCCGGG 1 cut(s) 59
CsiI ACCWGGT 1 cut(s) 732
Csp6I GTAC 3 cut(s) 193, 220, 572
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 8 cut(s) 185, 362, 502, 506, 629, 704, 832, 916
CviQI GTAC 3 cut(s) 193, 220, 572
DdeI CTNAG 3 cut(s) 333, 927, 981
DpnI GATC 6 cut(s) 92, 201, 273, 540, 612, 714
DpnII GATC 6 cut(s) 90, 199, 271, 538, 610, 712
DraIII CACNNNGTG 1 cut(s) 450
EaeI YGGCCR 1 cut(s) 560
Ecl136II GAGCTC 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 3 cut(s) 4, 70, 671
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 59
EcoICRI GAGCTC 1 cut(s) 151
EcoRII CCWGG 3 cut(s) 732, 931, 985
EcoT22I ATGCAT 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 153
FaeI CATG 8 cut(s) 188, 365, 505, 509, 632, 707, 835, 919
FatI CATG 8 cut(s) 184, 361, 501, 505, 628, 703, 831, 915
FauI CCCGC 2 cut(s) 642, 889
Fnu4HI GCNGC 8 cut(s) 98, 131, 134, 474, 560, 924, 975, 978
FokI GGATG 2 cut(s) 758, 857
FriOI GRGCYC 1 cut(s) 153
FseI GGCCGGCC 1 cut(s) 58
Fsp4HI GCNGC 8 cut(s) 98, 131, 134, 474, 560, 924, 975, 978
GluI GCNGC 8 cut(s) 98, 131, 134, 474, 560, 924, 975, 978
GsuI CTGGAG 1 cut(s) 969
HaeIII GGCC 3 cut(s) 54, 58, 562
HapII CCGG 3 cut(s) 55, 60, 94
Hin1II CATG 8 cut(s) 188, 365, 505, 509, 632, 707, 835, 919
HinfI GANTC 5 cut(s) 36, 83, 417, 493, 1005
HpaII CCGG 3 cut(s) 55, 60, 94
HphI GGTGA 3 cut(s) 188, 694, 722
Hpy166II GTNNAC 3 cut(s) 227, 587, 663
Hpy188I TCNGA 9 cut(s) 50, 82, 88, 199, 480, 548, 610, 699, 1017
Hpy188III TCNNGA 7 cut(s) 17, 166, 332, 349, 521, 674, 948
Hpy8I GTNNAC 3 cut(s) 227, 587, 663
HpyAV CCTTC 2 cut(s) 473, 608
HpyCH4III ACNGT 4 cut(s) 224, 383, 682, 997
HpyCH4V TGCA 8 cut(s) 130, 314, 371, 501, 505, 529, 923, 1021
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 73, 971
HpyF3I CTNAG 3 cut(s) 333, 927, 981
Hsp92II CATG 8 cut(s) 188, 365, 505, 509, 632, 707, 835, 919
KroI GCCGGC 1 cut(s) 54
KroNI GCCGGC 1 cut(s) 56
Kzo9I GATC 6 cut(s) 90, 199, 271, 538, 610, 712
LmnI GCTCC 1 cut(s) 912
Lsp1109I GCAGC 7 cut(s) 109, 142, 145, 485, 935, 986, 989
LweI GCATC 2 cut(s) 380, 1008
MabI ACCWGGT 1 cut(s) 732
MaeIII GTNAC 4 cut(s) 593, 682, 778, 997
MalI GATC 6 cut(s) 92, 201, 273, 540, 612, 714
MbiI CCGCTC 1 cut(s) 649
MboI GATC 6 cut(s) 90, 199, 271, 538, 610, 712
MboII GAAGA 5 cut(s) 115, 296, 426, 448, 686
MflI RGATCY 3 cut(s) 90, 199, 712
MhlI GDGCHC 3 cut(s) 153, 730, 863
MluCI AATT 8 cut(s) 139, 244, 337, 532, 550, 799, 812, 899
MlyI GAGTC 2 cut(s) 30, 92
MmeI TCCRAC 2 cut(s) 60, 66
MnlI CCTC 8 cut(s) 44, 50, 243, 345, 356, 420, 632, 736
Mph1103I ATGCAT 1 cut(s) 507
MroNI GCCGGC 1 cut(s) 54
MroXI GAANNNNTTC 4 cut(s) 341, 440, 492, 940
MseI TTAA 5 cut(s) 206, 456, 854, 875, 903
MslI CAYNNNNRTG 2 cut(s) 366, 856
MspA1I CMGCKG 2 cut(s) 76, 559
MspI CCGG 3 cut(s) 55, 60, 94
MspR9I CCNGG 5 cut(s) 60, 61, 734, 933, 987
MvaI CCWGG 3 cut(s) 734, 933, 987
MwoI GCNNNNNNNGC 3 cut(s) 64, 73, 971
NaeI GCCGGC 1 cut(s) 56
NciI CCSGG 2 cut(s) 60, 61
NdeII GATC 6 cut(s) 90, 199, 271, 538, 610, 712
NgoMIV GCCGGC 1 cut(s) 54
NlaIII CATG 8 cut(s) 188, 365, 505, 509, 632, 707, 835, 919
NlaIV GGNNCC 1 cut(s) 829
NmuCI GTSAC 3 cut(s) 593, 682, 778
NsiI ATGCAT 1 cut(s) 507
NspI RCATGY 1 cut(s) 505
PaeI GCATGC 1 cut(s) 505
PdiI GCCGGC 1 cut(s) 56
PdmI GAANNNNTTC 4 cut(s) 341, 440, 492, 940
PfeI GAWTC 3 cut(s) 417, 493, 1005
PflMI CCANNNNNTGG 1 cut(s) 739
PkrI GCNGC 8 cut(s) 99, 132, 135, 475, 561, 925, 976, 979
PleI GAGTC 2 cut(s) 30, 91
PpsI GAGTC 2 cut(s) 30, 91
PshBI ATTAAT 1 cut(s) 854
Psp124BI GAGCTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 732, 931, 985
PspGI CCWGG 3 cut(s) 732, 931, 985
PspN4I GGNNCC 1 cut(s) 829
PspPI GGNCC 4 cut(s) 4, 57, 70, 671
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 3 cut(s) 90, 199, 712
PvuII CAGCTG 1 cut(s) 76
RigI GGCCGGCC 1 cut(s) 58
RsaI GTAC 3 cut(s) 194, 221, 573
RsaNI GTAC 3 cut(s) 193, 220, 572
RseI CAYNNNNRTG 2 cut(s) 366, 856
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 5 cut(s) 206, 456, 854, 875, 903
SatI GCNGC 8 cut(s) 98, 131, 134, 474, 560, 924, 975, 978
Sau3AI GATC 6 cut(s) 90, 199, 271, 538, 610, 712
Sau96I GGNCC 4 cut(s) 4, 57, 70, 671
ScaI AGTACT 1 cut(s) 194
SchI GAGTC 2 cut(s) 30, 92
ScrFI CCNGG 5 cut(s) 60, 61, 734, 933, 987
SduI GDGCHC 3 cut(s) 153, 730, 863
SexAI ACCWGGT 1 cut(s) 732
SfaNI GCATC 2 cut(s) 380, 1008
SfcI CTRYAG 1 cut(s) 921
SinI GGWCC 3 cut(s) 4, 70, 671
SmaI CCCGGG 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 366, 856
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
SphI GCATGC 1 cut(s) 505
Sse9I AATT 8 cut(s) 139, 244, 337, 532, 550, 799, 812, 899
SsiI CCGC 3 cut(s) 559, 649, 882
SspI AATATT 1 cut(s) 873
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 5 cut(s) 58, 59, 732, 931, 985
TaaI ACNGT 4 cut(s) 224, 383, 682, 997
TaqI TCGA 3 cut(s) 42, 153, 216
TasI AATT 8 cut(s) 139, 244, 337, 532, 550, 799, 812, 899
TatI WGTACW 1 cut(s) 192
TauI GCSGC 1 cut(s) 562
TfiI GAWTC 3 cut(s) 417, 493, 1005
Tru1I TTAA 5 cut(s) 206, 456, 854, 875, 903
Tru9I TTAA 5 cut(s) 206, 456, 854, 875, 903
TscAI CASTG 2 cut(s) 474, 1000
TseFI GTSAC 3 cut(s) 593, 682, 778
TseI GCWGC 7 cut(s) 97, 130, 133, 473, 923, 974, 977
Tsp45I GTSAC 3 cut(s) 593, 682, 778
TspDTI ATGAA 4 cut(s) 378, 501, 617, 933
TspGWI ACGGA 2 cut(s) 405, 701
TspMI CCCGGG 1 cut(s) 59
TspRI CASTG 2 cut(s) 474, 1000
Van91I CCANNNNNTGG 1 cut(s) 739
VpaK11BI GGWCC 3 cut(s) 4, 70, 671
VspI ATTAAT 1 cut(s) 854
XapI RAATTY 4 cut(s) 337, 550, 812, 899
XceI RCATGY 1 cut(s) 505
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XmaI CCCGGG 1 cut(s) 59
XmnI GAANNNNTTC 4 cut(s) 341, 440, 492, 940
ZrmI AGTACT 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.