Rh3AG023200

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010655
Physical Location & Seq
Reverse (-)
1566067 .. 1570519
4453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG023200.1

Sequence Viewer

Length: 1083 bp
ATGGACCCATCGGTTCTTGATGACATCATCAGCCGTCTTCTTGAAGTCCGAGGCCGACCCGGAAAGCAGGTGCAGCTATCCGAGTCCGAGATCCGACAACTCTGCTCTGCCTCTAAAGATATCTTCTTGCAACAACCCAATTTGTTAGAGCTTGAAGCGCCCATCAAGATTTGTGGTGACATACATGGTCAATACTCGGATCTCTTAAGGCTTTTTGAGTATGGCGGATTACCTCCTAATTCCAATTACTTGTTCTTGGGAGATTATGTGGATCGAGGCAAGCAAAGTTTAGAAACAATATGTCTTCTCCTTGCATACAAAATAAAGTACCCTGAGAATTTTTTCCTCTTGAGAGGAAACCATGAATGTGCTTCTATAAACCGTATATATGGATTTTATGATGAGTGTAAGAGAAGATTCAATGTGAGGCTATGGAAGACATTTACGGATTGTTTCAACTGCCTACCAGTTGCAGCCCTGATTGATGAAAAGATTCTCTGCATGCATGGGGGTCTTTCTCCTGAGCTGAATAATTTAGATCAAATTAGATCTTTACACCGGCCAACTGATGTACCAGATACAGGTTTACTCTGTGATCTTCTCTGGTCTGATCCCAGTAAAGATGTTCAAGGTTGGGGAGGGAACGACCGGGGAGTGTCCTACACATTTGGTCCTGATACAGTGACGGAGTTTCTTGAGAAGCATGATTTGGATCTCGTTTGTCGTGCTCACCAGGTTGTGGAGGATGGGTATGAGTTATTTGCCAACCGCCAACTTGTGACAATATTTTCAGCACCTAATTATTGTGGGGAATTCGATAATGCTGGTGCCATGATGAGCGTGGATGACACGTTAATGTGCTCTTTCCAAATATTAAAACCTGCAGACAAGAAGGTGAAGTCTAACTTGGGTAGCCTAACTACAGCTAAGCCTGGAAATTCTTCTACTGGTGTTTTTGGGAGCACAACTACAGCTAAGCCTAGCAATACTCCAGCAGGAGTCAAGGTAAATGCAACCTCTTACCAAAGCAGAAATGAAATTAAGTTTGGTGAAGTAAGCTTTAATGGTACAATGGATGTTTAA

Protein Analysis

360

Amino Acids

40.14

Weight (kDa)

5.18

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STPPase_N PF16891 6 - 52 8.6e-23 Serine-threonine protein phosphatase N-terminal domain
Metallophos PF00149 54 - 247 2.8e-52 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010655)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29400 AT3G46820 AT5G59160 AT5G59160 AT5G59160
fragaria_vesca FvH4_6g02160 FvH4_6g02160
malus_domestica MD04G1229100.v1.1 MD12G1246200.v1.1
prunus_persica Prupe.6G349000_v2.0.a1
pyrus_communis pycom04g20300 pycom12g22570
rosa_chinensis RchiOBHm_Chr3g0449941
rosa_laevigata RLG00000025773
rosa_multiflora Rmu_sc0002646.1_g000002
rosa_roxburghii Rroxscaffold_6G00427730
rosa_rugosa Rorug02G0624100
rosa_samantha Rh3AG023200 Rh3DG023700
rosa_wichuraiana Rw3G001860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 2 cut(s) 58, 889
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 739
AciI CCGC 2 cut(s) 225, 769
AclWI GGATC 5 cut(s) 85, 207, 279, 605, 720
AcoI YGGCCR 1 cut(s) 560
AcsI RAATTY 3 cut(s) 337, 812, 937
AfaI GTAC 3 cut(s) 329, 573, 1069
AfiI CCNNNNNNNGG 2 cut(s) 581, 739
AflII CTTAAG 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 849
AgsI TTSAA 5 cut(s) 44, 155, 421, 457, 629
AjnI CCWGG 2 cut(s) 732, 931
AjuI GAANNNNNNNTTGG 8 cut(s) 236, 268, 692, 724, 890, 922, 1029, 1061
AluBI AGCT 6 cut(s) 76, 151, 526, 926, 974, 1059
AluI AGCT 6 cut(s) 76, 151, 526, 926, 974, 1059
Alw21I GWGCWC 3 cut(s) 730, 863, 965
AlwI GGATC 5 cut(s) 85, 207, 279, 605, 720
AoxI GGCC 2 cut(s) 52, 560
ApeKI GCWGC 2 cut(s) 73, 473
ApoI RAATTY 3 cut(s) 337, 812, 937
Asp700I GAANNNNTTC 3 cut(s) 341, 492, 940
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 2 cut(s) 4, 671
AsuC2I CCSGG 2 cut(s) 60, 650
AsuHPI GGTGA 4 cut(s) 188, 722, 907, 1061
AvaII GGWCC 2 cut(s) 4, 671
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BanI GGYRCC 1 cut(s) 827
BbsI GAAGAC 3 cut(s) 29, 296, 443
Bbv12I GWGCWC 3 cut(s) 730, 863, 965
BbvI GCAGC 2 cut(s) 85, 485
BccI CCATC 3 cut(s) 16, 170, 740
BceAI ACGGC 1 cut(s) 18
BcgI CGANNNNNNTGC 2 cut(s) 84, 118
BciT130I CCWGG 2 cut(s) 734, 933
BcnI CCSGG 2 cut(s) 60, 650
BfaI CTAG 1 cut(s) 981
BfmI CTRYAG 3 cut(s) 882, 921, 969
BfoI RGCGCY 1 cut(s) 161
BfrI CTTAAG 1 cut(s) 205
BfuAI ACCTGC 2 cut(s) 58, 889
BglII AGATCT 1 cut(s) 548
BisI GCNGC 2 cut(s) 74, 474
BlpI GCTNAGC 2 cut(s) 927, 975
BlsI GCNGC 2 cut(s) 75, 475
Bme1390I CCNGG 4 cut(s) 60, 650, 734, 933
Bme18I GGWCC 2 cut(s) 4, 671
BmgT120I GGNCC 2 cut(s) 4, 671
BmiI GGNNCC 2 cut(s) 6, 829
BmrFI CCNGG 4 cut(s) 60, 650, 734, 933
BmrI ACTGGG 1 cut(s) 609
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 3 cut(s) 29, 296, 443
BpmI CTGGAG 1 cut(s) 975
Bpu10I CCTNAGC 1 cut(s) 522
Bpu1102I GCTNAGC 2 cut(s) 927, 975
BpuEI CTTGAG 2 cut(s) 370, 716
BpuMI CCSGG 2 cut(s) 60, 650
BsaBI GATNNNNATC 1 cut(s) 711
BsaJI CCNNGG 2 cut(s) 49, 649
BsaXI ACNNNNNCTCC 6 cut(s) 252, 282, 734, 764, 990, 1020
Bsc4I CCNNNNNNNGG 2 cut(s) 581, 739
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 3 cut(s) 467, 615, 952
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 2 cut(s) 734, 933
BseDI CCNNGG 2 cut(s) 49, 649
BseGI GGATG 3 cut(s) 751, 850, 1081
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 2 cut(s) 581, 739
BseMII CTCAG 2 cut(s) 324, 513
BseNI ACTGG 3 cut(s) 467, 615, 952
BseXI GCAGC 2 cut(s) 85, 485
BsgI GTGCAG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 649
BshFI GGCC 2 cut(s) 54, 562
BshNI GGYRCC 1 cut(s) 827
BsiEI CGRYCG 1 cut(s) 649
BsiHKAI GWGCWC 3 cut(s) 730, 863, 965
BsiSI CCGG 3 cut(s) 60, 559, 649
BslI CCNNNNNNNGG 2 cut(s) 581, 739
BsnI GGCC 2 cut(s) 54, 562
Bsp1286I GDGCHC 3 cut(s) 730, 863, 965
Bsp143I GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
Bsp1720I GCTNAGC 2 cut(s) 927, 975
BspACI CCGC 2 cut(s) 225, 769
BspANI GGCC 2 cut(s) 54, 562
BspCNI CTCAG 2 cut(s) 325, 514
BspLI GGNNCC 2 cut(s) 6, 829
BspMAI CTGCAG 1 cut(s) 886
BspMI ACCTGC 2 cut(s) 58, 889
BspPI GGATC 5 cut(s) 85, 207, 279, 605, 720
BspT107I GGYRCC 1 cut(s) 827
BspTI CTTAAG 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 558
BsrI ACTGG 3 cut(s) 467, 615, 952
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 2 cut(s) 49, 649
BssMI GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
Bst2UI CCWGG 2 cut(s) 734, 933
Bst4CI ACNGT 2 cut(s) 383, 682
BstAFI CTTAAG 1 cut(s) 205
BstC8I GCNNGC 2 cut(s) 281, 503
BstDEI CTNAG 4 cut(s) 333, 522, 927, 975
BstF5I GGATG 3 cut(s) 751, 850, 1081
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 8 cut(s) 93, 202, 274, 541, 551, 598, 613, 715
BstMBI GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 2 cut(s) 73, 157
BstNI CCWGG 2 cut(s) 734, 933
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 4 cut(s) 58, 648, 732, 931
BstSFI CTRYAG 3 cut(s) 882, 921, 969
BstV1I GCAGC 2 cut(s) 85, 485
BstV2I GAAGAC 3 cut(s) 29, 296, 443
BstX2I RGATCY 4 cut(s) 90, 199, 548, 712
BstYI RGATCY 4 cut(s) 90, 199, 548, 712
BsuRI GGCC 2 cut(s) 54, 562
BtsCI GGATG 3 cut(s) 751, 850, 1081
BtsIMutI CAGTG 1 cut(s) 687
BveI ACCTGC 2 cut(s) 58, 889
Cac8I GCNNGC 2 cut(s) 281, 503
CfoI GCGC 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 2 cut(s) 4, 671
CsiI ACCWGGT 1 cut(s) 732
Csp6I GTAC 3 cut(s) 328, 572, 1068
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 6 cut(s) 185, 362, 502, 506, 704, 832
CviQI GTAC 3 cut(s) 328, 572, 1068
DdeI CTNAG 4 cut(s) 333, 522, 927, 975
DpnI GATC 8 cut(s) 92, 201, 273, 540, 550, 597, 612, 714
DpnII GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
EaeI YGGCCR 1 cut(s) 560
EciI GGCGGA 1 cut(s) 240
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 2 cut(s) 4, 671
EcoRI GAATTC 1 cut(s) 812
EcoRII CCWGG 2 cut(s) 732, 931
EcoRV GATATC 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 507
FaeI CATG 6 cut(s) 188, 365, 505, 509, 707, 835
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FatI CATG 6 cut(s) 184, 361, 501, 505, 703, 831
Fnu4HI GCNGC 2 cut(s) 74, 474
FokI GGATG 2 cut(s) 758, 857
Fsp4HI GCNGC 2 cut(s) 74, 474
FspBI CTAG 1 cut(s) 981
GlaI GCGC 1 cut(s) 159
GluI GCNGC 2 cut(s) 74, 474
GsuI CTGGAG 1 cut(s) 975
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 2 cut(s) 54, 562
HapII CCGG 3 cut(s) 60, 559, 649
HhaI GCGC 1 cut(s) 160
Hin1II CATG 6 cut(s) 188, 365, 505, 509, 707, 835
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HindIII AAGCTT 1 cut(s) 1057
HinfI GANTC 4 cut(s) 83, 417, 493, 999
HpaII CCGG 3 cut(s) 60, 559, 649
HphI GGTGA 4 cut(s) 188, 722, 907, 1061
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 6 cut(s) 50, 82, 88, 95, 199, 610
Hpy188III TCNNGA 7 cut(s) 17, 41, 166, 349, 521, 674, 695
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 1 cut(s) 886
HpyCH4III ACNGT 2 cut(s) 383, 682
HpyCH4IV ACGT 1 cut(s) 851
HpyCH4V TGCA 8 cut(s) 73, 130, 314, 473, 501, 505, 884, 1013
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 157
HpyF3I CTNAG 4 cut(s) 333, 522, 927, 975
HpySE526I ACGT 1 cut(s) 851
Hsp92II CATG 6 cut(s) 188, 365, 505, 509, 707, 835
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
LmnI GCTCC 1 cut(s) 960
Lsp1109I GCAGC 2 cut(s) 85, 485
MabI ACCWGGT 1 cut(s) 732
MaeI CTAG 1 cut(s) 981
MaeII ACGT 1 cut(s) 851
MaeIII GTNAC 3 cut(s) 176, 682, 778
MalI GATC 8 cut(s) 92, 201, 273, 540, 550, 597, 612, 714
MboI GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
MboII GAAGA 7 cut(s) 29, 115, 296, 426, 448, 590, 933
MflI RGATCY 4 cut(s) 90, 199, 548, 712
MhlI GDGCHC 3 cut(s) 730, 863, 965
MlyI GAGTC 2 cut(s) 92, 1008
MmeI TCCRAC 1 cut(s) 118
Mph1103I ATGCAT 1 cut(s) 507
MroXI GAANNNNTTC 3 cut(s) 341, 492, 940
MseI TTAA 6 cut(s) 206, 854, 875, 1041, 1062, 1081
MslI CAYNNNNRTG 2 cut(s) 366, 854
MspCI CTTAAG 1 cut(s) 205
MspI CCGG 3 cut(s) 60, 559, 649
MspR9I CCNGG 4 cut(s) 60, 650, 734, 933
MvaI CCWGG 2 cut(s) 734, 933
MwoI GCNNNNNNNGC 2 cut(s) 73, 157
NciI CCSGG 2 cut(s) 60, 650
NdeII GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
NlaIII CATG 6 cut(s) 188, 365, 505, 509, 707, 835
NlaIV GGNNCC 2 cut(s) 6, 829
NmuCI GTSAC 3 cut(s) 176, 682, 778
NsiI ATGCAT 1 cut(s) 507
NspI RCATGY 1 cut(s) 505
PaeI GCATGC 1 cut(s) 505
PaqCI CACCTGC 1 cut(s) 58
PdmI GAANNNNTTC 3 cut(s) 341, 492, 940
PfeI GAWTC 2 cut(s) 417, 493
PflMI CCANNNNNTGG 1 cut(s) 739
PkrI GCNGC 2 cut(s) 75, 475
PleI GAGTC 2 cut(s) 91, 1007
PpsI GAGTC 2 cut(s) 91, 1007
Psp6I CCWGG 2 cut(s) 732, 931
PspGI CCWGG 2 cut(s) 732, 931
PspN4I GGNNCC 2 cut(s) 6, 829
PspPI GGNCC 2 cut(s) 4, 671
PstI CTGCAG 1 cut(s) 886
PsuI RGATCY 4 cut(s) 90, 199, 548, 712
RsaI GTAC 3 cut(s) 329, 573, 1069
RsaNI GTAC 3 cut(s) 328, 572, 1068
RseI CAYNNNNRTG 2 cut(s) 366, 854
SaqAI TTAA 6 cut(s) 206, 854, 875, 1041, 1062, 1081
SatI GCNGC 2 cut(s) 74, 474
Sau3AI GATC 8 cut(s) 90, 199, 271, 538, 548, 595, 610, 712
Sau96I GGNCC 2 cut(s) 4, 671
SchI GAGTC 2 cut(s) 92, 1008
ScrFI CCNGG 4 cut(s) 60, 650, 734, 933
SduI GDGCHC 3 cut(s) 730, 863, 965
SexAI ACCWGGT 1 cut(s) 732
SfcI CTRYAG 3 cut(s) 882, 921, 969
SinI GGWCC 2 cut(s) 4, 671
SmiMI CAYNNNNRTG 2 cut(s) 366, 854
SmlI CTYRAG 3 cut(s) 205, 349, 695
SmoI CTYRAG 3 cut(s) 205, 349, 695
SphI GCATGC 1 cut(s) 505
SsiI CCGC 2 cut(s) 225, 769
SspI AATATT 2 cut(s) 786, 873
SspMI CTAG 1 cut(s) 981
StyD4I CCNGG 4 cut(s) 58, 648, 732, 931
TaaI ACNGT 2 cut(s) 383, 682
TaiI ACGT 1 cut(s) 854
TaqI TCGA 2 cut(s) 274, 816
TfiI GAWTC 2 cut(s) 417, 493
Tru1I TTAA 6 cut(s) 206, 854, 875, 1041, 1062, 1081
Tru9I TTAA 6 cut(s) 206, 854, 875, 1041, 1062, 1081
TscAI CASTG 1 cut(s) 687
TseFI GTSAC 3 cut(s) 176, 682, 778
TseI GCWGC 2 cut(s) 73, 473
Tsp45I GTSAC 3 cut(s) 176, 682, 778
TspDTI ATGAA 3 cut(s) 378, 501, 1050
TspGWI ACGGA 2 cut(s) 461, 701
TspRI CASTG 1 cut(s) 687
Van91I CCANNNNNTGG 1 cut(s) 739
Vha464I CTTAAG 1 cut(s) 205
VpaK11BI GGWCC 2 cut(s) 4, 671
XapI RAATTY 3 cut(s) 337, 812, 937
XceI RCATGY 1 cut(s) 505
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XmnI GAANNNNTTC 3 cut(s) 341, 492, 940
XspI CTAG 1 cut(s) 981
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.