MD05G1008900.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015543
Physical Location & Seq
Reverse (-)
1935938 .. 1939970
4033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1008900.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCTGCTGAGACAGCTCCAGTGCTTATTAATCATATGACTATTCAACCCAACCCTGCCATCTCCCTTCCCTCCATTAACTCACCTTCCATCATCAACCCTAATGATTCTGTTGTAGCTGTTGGGGCTGATGAAATCCCCACCATTGATTATATCATGTTATTCTCTAATGATTTTGATGAACGATCCAAAGCCCTCGAAAACTTAGGCCATGTCTGCAAGGACTTCGGCTTTTTCTATCTTGTAAATCATGGCATCTCCAACGGCGTGTTTGAGGGTGTTTTCAAAGGAATTTCTGACTTCTTTAACCCGGAGGAGATAGAGGACCGAAAGCAGTACGAGAAAAAGAGCCCAACGGATAGAATTCGATGGGGCTTGCGATCTTCTCCTGGAGAGAATAGGGAATATCTGAAGGTCATTGCACATCCCCAGTTTCACTGCCCTTCTAAACCAGCTGGTTTCAGTGACGCTTTGGAAGAGTATTTCAAAGCATCGCGAGAGATAGTAAAAGGTTTGGGAAAGGCAGTGTCAAAAGGCTTGGGATTTGAAGAATGCTACGTAGAAAAAGCTTTTAAGCTTGAATCTGGTTTTGATATGTCTGCCATGAACCTATACCCACCCAATTTCAGATCAAAAGGATCAATTGGTGTGCCTGATCATACTGACCCAGGCTTCTTTGTGTCACTCATACAAGATGTGAATGGTGGTCTGCAAATACTATCCAATAGTGGTCAATGCATCAATGTTAATATCCCCCATAATGCCATTTTTATAAACCTTGGTGACCATCTTGAGATCTTAACCAATGGGAAGTACAAGAGCCATGTTCATCGAGTGGTGGTGGACAACAACAAAATACAGAGGATCTCGGTGGCAACACTACATGGACCATCGTTGGATGCACTAGTGGGACCAGCACCAGAGTTTGTAGATGAATGTCACCCACCAGCATATCGTGCAATGACCTACAAACAATCCATAGAAGCTAATGGCGGTGATGAGATTGATGTCCAGTCAAGCATCGACCAAATTCGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

347

Amino Acids

38.19

Weight (kDa)

5.37

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 46 - 118 6.6e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 199 - 296 4.5e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015543)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15310 FvH4_2g15312
malus_domestica MD05G1008900.v1.1
prunus_persica Prupe.8G011000_v2.0.a1 Prupe.8G011200_v2.0.a1
pyrus_communis pycom05g00460
rosa_chinensis RchiOBHm_Chr6g0278051
rosa_laevigata RLG00000013242
rosa_multiflora Rmu_sc0000791.1_g000018
rosa_roxburghii Rroxscaffold_7G00190560
rosa_rugosa Rorug06G0114500
rosa_samantha Rh6AG224300 Rh6BG228300 Rh6CG230700 Rh6DG221800
rosa_wichuraiana Rw6G019580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AccII CGCG 1 cut(s) 496
AciI CCGC 1 cut(s) 993
AclWI GGATC 3 cut(s) 180, 646, 872
AcsI RAATTY 3 cut(s) 291, 363, 1029
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 2 cut(s) 338, 815
AgsI TTSAA 5 cut(s) 47, 286, 487, 548, 581
AhlI ACTAGT 1 cut(s) 904
AjnI CCWGG 2 cut(s) 388, 667
AluBI AGCT 6 cut(s) 17, 119, 455, 569, 577, 986
AluI AGCT 6 cut(s) 17, 119, 455, 569, 577, 986
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 3 cut(s) 180, 646, 872
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 3 cut(s) 291, 363, 1029
AseI ATTAAT 1 cut(s) 30
AspS9I GGNCC 3 cut(s) 325, 887, 911
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 4 cut(s) 75, 794, 932, 1007
AvaII GGWCC 3 cut(s) 325, 887, 911
BanII GRGCYC 1 cut(s) 353
BccI CCATC 5 cut(s) 68, 98, 363, 795, 898
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 2 cut(s) 390, 669
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 5
BcuI ACTAGT 1 cut(s) 904
BfaI CTAG 1 cut(s) 905
BglII AGATCT 1 cut(s) 795
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 3 cut(s) 311, 390, 669
Bme18I GGWCC 3 cut(s) 325, 887, 911
BmgT120I GGNCC 3 cut(s) 325, 887, 911
BmiI GGNNCC 1 cut(s) 912
BmrFI CCNGG 3 cut(s) 311, 390, 669
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 5 cut(s) 264, 500, 747, 889, 1029
BmuI ACTGGG 1 cut(s) 424
BpmI CTGGAG 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 559
BsaJI CCNNGG 2 cut(s) 667, 778
Bse1I ACTGG 3 cut(s) 20, 430, 1012
Bse3DI GCAATG 2 cut(s) 417, 966
BseBI CCWGG 2 cut(s) 390, 669
BseDI CCNNGG 2 cut(s) 667, 778
BseGI GGATG 2 cut(s) 424, 904
BseMI GCAATG 2 cut(s) 417, 966
BseNI ACTGG 3 cut(s) 20, 430, 1012
BseRI GAGGAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 496
BshFI GGCC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 924
BsmAI GTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 924
BsmI GAATGC 1 cut(s) 557
BsnI GGCC 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
Bsp68I TCGCGA 1 cut(s) 496
BspACI CCGC 1 cut(s) 993
BspANI GGCC 1 cut(s) 210
BspFNI CGCG 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 912
BspPI GGATC 3 cut(s) 180, 646, 872
BsrDI GCAATG 2 cut(s) 417, 966
BsrI ACTGG 3 cut(s) 20, 430, 1012
BssECI CCNNGG 2 cut(s) 667, 778
BssMI GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
BssT1I CCWWGG 1 cut(s) 778
Bst2UI CCWGG 2 cut(s) 390, 669
Bst6I CTCTTC 1 cut(s) 471
BstAPI GCANNNNNTGC 1 cut(s) 956
BstBAI YACGTR 1 cut(s) 559
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 9, 205
BstEII GGTNACC 1 cut(s) 782
BstF5I GGATG 2 cut(s) 424, 904
BstFNI CGCG 1 cut(s) 496
BstKTI GATC 7 cut(s) 188, 383, 632, 641, 658, 798, 867
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
BstMWI GCNNNNNNNGC 4 cut(s) 14, 125, 216, 956
BstNI CCWGG 2 cut(s) 390, 669
BstPI GGTNACC 1 cut(s) 782
BstSCI CCNGG 3 cut(s) 309, 388, 667
BstSNI TACGTA 1 cut(s) 559
BstUI CGCG 1 cut(s) 496
BstX2I RGATCY 2 cut(s) 795, 864
BstYI RGATCY 2 cut(s) 795, 864
BsuRI GGCC 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 424, 904
BtsI GCAGTG 2 cut(s) 436, 531
BtsIMutI CAGTG 4 cut(s) 27, 436, 469, 531
BtuMI TCGCGA 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 377
Cfr13I GGNCC 3 cut(s) 325, 887, 911
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 337, 814
CviAII CATG 6 cut(s) 157, 212, 251, 604, 824, 884
CviQI GTAC 2 cut(s) 337, 814
DdeI CTNAG 2 cut(s) 9, 205
DpnI GATC 7 cut(s) 187, 382, 631, 640, 657, 797, 866
DpnII GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco105I TACGTA 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 3 cut(s) 325, 887, 911
Eco57I CTGAAG 1 cut(s) 431
Eco91I GGTNACC 1 cut(s) 782
EcoO65I GGTNACC 1 cut(s) 782
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 2 cut(s) 388, 667
EcoT14I CCWWGG 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 740
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 778
FaeI CATG 6 cut(s) 160, 215, 254, 607, 827, 887
FaqI GGGAC 1 cut(s) 924
FatI CATG 6 cut(s) 156, 211, 250, 603, 823, 883
FauNDI CATATG 1 cut(s) 36
FbaI TGATCA 1 cut(s) 655
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 411, 911
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 905
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 476
Hin1II CATG 6 cut(s) 160, 215, 254, 607, 827, 887
HindIII AAGCTT 2 cut(s) 567, 575
HinfI GANTC 2 cut(s) 107, 581
HpaII CCGG 1 cut(s) 311
HphI GGTGA 4 cut(s) 75, 794, 932, 1007
Hpy166II GTNNAC 1 cut(s) 844
Hpy188I TCNGA 3 cut(s) 298, 411, 629
Hpy188III TCNNGA 2 cut(s) 495, 791
Hpy8I GTNNAC 1 cut(s) 844
HpyAV CCTTC 4 cut(s) 77, 96, 406, 453
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 6 cut(s) 219, 422, 712, 738, 902, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 125, 216, 956
HpyF3I CTNAG 2 cut(s) 9, 205
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 6 cut(s) 160, 215, 254, 607, 827, 887
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
LmnI GCTCC 1 cut(s) 22
LweI GCATC 5 cut(s) 264, 500, 747, 889, 1029
MaeI CTAG 1 cut(s) 905
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 4 cut(s) 464, 681, 782, 938
MalI GATC 7 cut(s) 187, 382, 631, 640, 657, 797, 866
MboI GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
MboII GAAGA 3 cut(s) 375, 488, 560
MfeI CAATTG 1 cut(s) 642
MflI RGATCY 2 cut(s) 795, 864
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 5 cut(s) 291, 363, 622, 642, 1029
MmeI TCCRAC 2 cut(s) 285, 876
MnlI CCTC 6 cut(s) 82, 206, 268, 307, 316, 855
Mph1103I ATGCAT 1 cut(s) 740
MseI TTAA 6 cut(s) 30, 78, 306, 573, 747, 800
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 3 cut(s) 311, 390, 669
MunI CAATTG 1 cut(s) 642
Mva1269I GAATGC 1 cut(s) 557
MvaI CCWGG 2 cut(s) 390, 669
MvnI CGCG 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 14, 125, 216, 956
NciI CCSGG 1 cut(s) 311
NdeI CATATG 1 cut(s) 36
NdeII GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
NlaIII CATG 6 cut(s) 160, 215, 254, 607, 827, 887
NlaIV GGNNCC 1 cut(s) 912
NmuCI GTSAC 4 cut(s) 464, 681, 782, 938
NruI TCGCGA 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 740
PctI GAATGC 1 cut(s) 557
PfeI GAWTC 2 cut(s) 107, 581
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 559
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 773
Psp6I CCWGG 2 cut(s) 388, 667
PspEI GGTNACC 1 cut(s) 782
PspGI CCWGG 2 cut(s) 388, 667
PspN4I GGNNCC 1 cut(s) 912
PspPI GGNCC 3 cut(s) 325, 887, 911
PsuI RGATCY 2 cut(s) 795, 864
PvuII CAGCTG 1 cut(s) 455
RruI TCGCGA 1 cut(s) 496
RsaI GTAC 2 cut(s) 338, 815
RsaNI GTAC 2 cut(s) 337, 814
SaqAI TTAA 6 cut(s) 30, 78, 306, 573, 747, 800
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 7 cut(s) 185, 380, 629, 638, 655, 795, 864
Sau96I GGNCC 3 cut(s) 325, 887, 911
ScrFI CCNGG 3 cut(s) 311, 390, 669
SduI GDGCHC 1 cut(s) 353
SfaNI GCATC 5 cut(s) 264, 500, 747, 889, 1029
SinI GGWCC 3 cut(s) 325, 887, 911
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
SnaBI TACGTA 1 cut(s) 559
SpeI ACTAGT 1 cut(s) 904
Sse9I AATT 5 cut(s) 291, 363, 622, 642, 1029
SsiI CCGC 1 cut(s) 993
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 3 cut(s) 309, 388, 667
StyI CCWWGG 1 cut(s) 778
TaiI ACGT 1 cut(s) 561
TaqI TCGA 4 cut(s) 198, 367, 832, 1023
TaqII GACCGA 1 cut(s) 342
TasI AATT 5 cut(s) 291, 363, 622, 642, 1029
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 2 cut(s) 107, 581
Tru1I TTAA 6 cut(s) 30, 78, 306, 573, 747, 800
Tru9I TTAA 6 cut(s) 30, 78, 306, 573, 747, 800
TscAI CASTG 4 cut(s) 27, 443, 469, 531
TseFI GTSAC 4 cut(s) 464, 681, 782, 938
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 4 cut(s) 464, 681, 782, 938
TspDTI ATGAA 5 cut(s) 147, 195, 620, 818, 948
TspGWI ACGGA 1 cut(s) 371
TspRI CASTG 4 cut(s) 27, 443, 469, 531
VpaK11BI GGWCC 3 cut(s) 325, 887, 911
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 3 cut(s) 291, 363, 1029
XspI CTAG 1 cut(s) 905
Zsp2I ATGCAT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.