Prupe.8G011200_v2.0.a1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0015543
Physical Location & Seq
Reverse (-)
919277 .. 922002
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G011200.1

Sequence Viewer

Length: 1164 bp
ATGTATTCTCCTTATAAATTGGAGGAACGTATTGGCATGAATGCATGCAAAACTAATCTACTTGTCTCCCATTGCTCTTCCTTGTTAAGATCAACTAAATCTTTCTACCTCTCCAAACACACAAACAAAATGGCTGAGACAGCTCCAGTTCTTGTTAACCACAGGCCTTTTGAACCAAACCCTATCCTCTCCCTTCACTCCATTGACTCATCCATCATCAAACCTCATGATTCCATTGCGGTTGTTGATGATGTTGAGATCCCCACCGTCGATTACTTCATGTTATTCTCTGATGATCTCGATGAACGATCCAAAGCCCTCGAATACATAGGCCATGTGTGCAAGGACTTTGGCTTCTTCTATCTAGTAAACCATGGCATATCCGATAGCGTGTTTGAGGGTGTTTTCAAAGGAATTTCTGATTTCTTTAATCCAACGGAGATAGAGAGCCGAAAGCAGTACGAGAAAAAGAATCCAACAAATAGAATTCGATGGGGCTTACGATCTTCTCCTGGAGAGAATAGGGAATATCTCAAGATCATTGCGCATCCCCAGTATCACTGCCCTACTAAACCATCTGGTTTCAGTGAGTCCATGGAAGAGTATTTCAAGGGCTTGCGAGAGGTAGTACAGGGTTTGGGAAAGGCAGTGTCAAAAGCCTTGGGATTTGAAGAATGCTACATAGAAAAAGCTTTTAAGCTTGGAACGGGCTTTGATGTGTCTGCCATGAACCTCTACCCTCCAAATTTCAGATCAAAAGGTTCCATTGGTGTACCTGACCATACTGACCCTGGCTTCTTTGTTTCACTCATTCAAGATGTGAATGGTGGCCTACAAGTATTTTCCAATGATGGAAACTGCATCAAAGTCAATATGCCCCCTAATGCCATTTTTATAAACCTTGGGGACCATCTTGAGATATTGACAAATGGTAAATACAAGAGCCATGTTCACCGAGTGGTTGTGGACAACAACAAAGTGAAGAGGATCTCAGTGGCTACGCTTCATGGACCTTCATTGGATGCATTTGTGAGCCCAGCACCAGAGTTTGTGGATGACTCTCACCCACCTGCATATCGTGGAATGACCTACAAGCAATCCTTACAAGCTAATGGCTCCGATGAGATCGATGTCCAATCTAGCATCGAACAAATTCGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

388

Amino Acids

43.41

Weight (kDa)

6.21

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015543)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15310 FvH4_2g15312
malus_domestica MD05G1008900.v1.1
prunus_persica Prupe.8G011000_v2.0.a1 Prupe.8G011200_v2.0.a1
pyrus_communis pycom05g00460
rosa_chinensis RchiOBHm_Chr6g0278051
rosa_laevigata RLG00000013242
rosa_multiflora Rmu_sc0000791.1_g000018
rosa_roxburghii Rroxscaffold_7G00190560
rosa_rugosa Rorug06G0114500
rosa_samantha Rh6AG224300 Rh6BG228300 Rh6CG230700 Rh6DG221800
rosa_wichuraiana Rw6G019580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 896
AarI CACCTGC 1 cut(s) 1078
Acc16I TGCGCA 1 cut(s) 546
Acc36I ACCTGC 1 cut(s) 1078
AciI CCGC 1 cut(s) 239
AclWI GGATC 3 cut(s) 253, 303, 995
AcsI RAATTY 4 cut(s) 414, 486, 745, 1152
AdeI CACNNNGTG 1 cut(s) 958
AfaI GTAC 3 cut(s) 461, 630, 774
AgsI TTSAA 5 cut(s) 173, 409, 610, 671, 815
AjnI CCWGG 2 cut(s) 511, 790
AluBI AGCT 4 cut(s) 143, 692, 700, 1109
AluI AGCT 4 cut(s) 143, 692, 700, 1109
Alw26I GTCTC 2 cut(s) 70, 131
AlwI GGATC 3 cut(s) 253, 303, 995
AoxI GGCC 3 cut(s) 164, 331, 829
ApoI RAATTY 4 cut(s) 414, 486, 745, 1152
Asp700I GAANNNNTTC 1 cut(s) 1152
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 2 cut(s) 907, 1010
AsuHPI GGTGA 2 cut(s) 944, 1055
AvaII GGWCC 2 cut(s) 907, 1010
BanII GRGCYC 1 cut(s) 1037
BccI CCATC 5 cut(s) 221, 486, 583, 845, 918
BciT130I CCWGG 2 cut(s) 513, 792
BcoDI GTCTC 2 cut(s) 70, 131
BfaI CTAG 2 cut(s) 365, 1140
BfuAI ACCTGC 1 cut(s) 1078
Bme1390I CCNGG 2 cut(s) 513, 792
Bme18I GGWCC 2 cut(s) 907, 1010
BmgT120I GGNCC 2 cut(s) 907, 1010
BmiI GGNNCC 3 cut(s) 763, 908, 1117
BmrFI CCNGG 2 cut(s) 513, 792
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 4 cut(s) 556, 870, 1012, 1152
BmuI ACTGGG 1 cut(s) 547
BpmI CTGGAG 2 cut(s) 129, 534
BpuEI CTTGAG 2 cut(s) 518, 935
Bsa29I ATCGAT 1 cut(s) 1128
BsaJI CCNNGG 5 cut(s) 373, 594, 660, 790, 901
Bse1I ACTGG 2 cut(s) 146, 553
Bse3DI GCAATG 3 cut(s) 70, 234, 540
BseBI CCWGG 2 cut(s) 513, 792
BseCI ATCGAT 1 cut(s) 1128
BseDI CCNNGG 5 cut(s) 373, 594, 660, 790, 901
BseGI GGATG 4 cut(s) 209, 547, 1027, 1060
BseMI GCAATG 3 cut(s) 70, 234, 540
BseMII CTCAG 2 cut(s) 126, 1005
BseNI ACTGG 2 cut(s) 146, 553
BseYI CCCAGC 1 cut(s) 1036
BshFI GGCC 3 cut(s) 166, 333, 831
BshVI ATCGAT 1 cut(s) 1128
BslFI GGGAC 1 cut(s) 920
BsmAI GTCTC 2 cut(s) 70, 131
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 2 cut(s) 46, 680
BsnI GGCC 3 cut(s) 166, 333, 831
Bsp1286I GDGCHC 1 cut(s) 1037
Bsp143I GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
Bsp19I CCATGG 2 cut(s) 373, 594
BspACI CCGC 1 cut(s) 239
BspANI GGCC 3 cut(s) 166, 333, 831
BspCNI CTCAG 2 cut(s) 127, 1004
BspDI ATCGAT 1 cut(s) 1128
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 3 cut(s) 763, 908, 1117
BspMI ACCTGC 1 cut(s) 1078
BspPI GGATC 3 cut(s) 253, 303, 995
BspQI GCTCTTC 1 cut(s) 82
BsrDI GCAATG 3 cut(s) 70, 234, 540
BsrI ACTGG 2 cut(s) 146, 553
BssECI CCNNGG 5 cut(s) 373, 594, 660, 790, 901
BssMI GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
BssT1I CCWWGG 4 cut(s) 373, 594, 660, 901
Bst2UI CCWGG 2 cut(s) 513, 792
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 3 cut(s) 82, 594, 977
BstC8I GCNNGC 2 cut(s) 46, 617
BstDEI CTNAG 2 cut(s) 135, 991
BstDSI CCRYGG 2 cut(s) 373, 594
BstF5I GGATG 4 cut(s) 209, 547, 1027, 1060
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 9 cut(s) 92, 261, 298, 311, 506, 540, 755, 990, 1128
BstMAI GTCTC 2 cut(s) 70, 131
BstMBI GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
BstMWI GCNNNNNNNGC 2 cut(s) 140, 339
BstNI CCWGG 2 cut(s) 513, 792
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 2 cut(s) 511, 790
BstX2I RGATCY 2 cut(s) 258, 987
BstYI RGATCY 2 cut(s) 258, 987
Bsu15I ATCGAT 1 cut(s) 1128
BsuRI GGCC 3 cut(s) 166, 333, 831
BsuTUI ATCGAT 1 cut(s) 1128
BtgI CCRYGG 2 cut(s) 373, 594
BtsCI GGATG 4 cut(s) 209, 547, 1027, 1060
BtsI GCAGTG 2 cut(s) 559, 654
BtsIMutI CAGTG 4 cut(s) 559, 592, 654, 999
BveI ACCTGC 1 cut(s) 1078
Cac8I GCNNGC 2 cut(s) 46, 617
CciI TCATGA 1 cut(s) 226
CfoI GCGC 1 cut(s) 547
Cfr13I GGNCC 2 cut(s) 907, 1010
ClaI ATCGAT 1 cut(s) 1128
Csp6I GTAC 3 cut(s) 460, 629, 773
CviQI GTAC 3 cut(s) 460, 629, 773
DdeI CTNAG 2 cut(s) 135, 991
DpnI GATC 9 cut(s) 91, 260, 297, 310, 505, 539, 754, 989, 1127
DpnII GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
DraIII CACNNNGTG 1 cut(s) 958
Eam1104I CTCTTC 3 cut(s) 82, 594, 977
EarI CTCTTC 3 cut(s) 82, 594, 977
Eco130I CCWWGG 4 cut(s) 373, 594, 660, 901
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 1037
Eco47I GGWCC 2 cut(s) 907, 1010
EcoRI GAATTC 1 cut(s) 486
EcoRII CCWGG 2 cut(s) 511, 790
EcoT14I CCWWGG 4 cut(s) 373, 594, 660, 901
EcoT22I ATGCAT 2 cut(s) 46, 1027
EcoT38I GRGCYC 1 cut(s) 1037
ErhI CCWWGG 4 cut(s) 373, 594, 660, 901
FalI AAGNNNNNCTT 2 cut(s) 1085, 1117
FaqI GGGAC 1 cut(s) 920
FokI GGATG 4 cut(s) 196, 534, 1034, 1067
FriOI GRGCYC 1 cut(s) 1037
FspBI CTAG 2 cut(s) 365, 1140
FspI TGCGCA 1 cut(s) 546
GlaI GCGC 1 cut(s) 546
GsaI CCCAGC 1 cut(s) 1040
GsuI CTGGAG 2 cut(s) 129, 534
HaeIII GGCC 3 cut(s) 166, 333, 831
HhaI GCGC 1 cut(s) 547
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 2 cut(s) 690, 698
HinfI GANTC 5 cut(s) 206, 230, 472, 590, 1058
HpaI GTTAAC 1 cut(s) 157
HphI GGTGA 2 cut(s) 944, 1055
Hpy166II GTNNAC 5 cut(s) 157, 370, 773, 952, 967
Hpy188I TCNGA 5 cut(s) 292, 385, 421, 752, 1120
Hpy188III TCNNGA 5 cut(s) 227, 299, 535, 815, 914
Hpy8I GTNNAC 5 cut(s) 157, 370, 773, 952, 967
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 2 cut(s) 203, 1023
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 6 cut(s) 44, 48, 342, 861, 1025, 1073
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 339
HpyF3I CTNAG 2 cut(s) 135, 991
HpySE526I ACGT 1 cut(s) 28
HspAI GCGC 1 cut(s) 545
KspAI GTTAAC 1 cut(s) 157
Kzo9I GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 148, 1121
LweI GCATC 4 cut(s) 556, 870, 1012, 1152
MaeI CTAG 2 cut(s) 365, 1140
MaeII ACGT 1 cut(s) 28
MalI GATC 9 cut(s) 91, 260, 297, 310, 505, 539, 754, 989, 1127
MboI GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
MboII GAAGA 6 cut(s) 69, 349, 498, 611, 683, 994
MflI RGATCY 2 cut(s) 258, 987
MhlI GDGCHC 1 cut(s) 1037
MluCI AATT 5 cut(s) 17, 414, 486, 745, 1152
MlyI GAGTC 3 cut(s) 200, 599, 1052
MmeI TCCRAC 2 cut(s) 458, 500
Mph1103I ATGCAT 2 cut(s) 46, 1027
MroXI GAANNNNTTC 1 cut(s) 1152
MseI TTAA 5 cut(s) 86, 156, 429, 696, 1162
MspR9I CCNGG 2 cut(s) 513, 792
Mva1269I GAATGC 2 cut(s) 46, 680
MvaI CCWGG 2 cut(s) 513, 792
MwoI GCNNNNNNNGC 2 cut(s) 140, 339
NcoI CCATGG 2 cut(s) 373, 594
NdeII GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
NlaIV GGNNCC 3 cut(s) 763, 908, 1117
NsbI TGCGCA 1 cut(s) 546
NsiI ATGCAT 2 cut(s) 46, 1027
NspI RCATGY 1 cut(s) 48
PaeI GCATGC 1 cut(s) 48
PagI TCATGA 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 1078
PceI AGGCCT 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 82
PctI GAATGC 2 cut(s) 46, 680
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 2 cut(s) 230, 472
PfoI TCCNGGA 1 cut(s) 511
PleI GAGTC 3 cut(s) 200, 598, 1052
PpsI GAGTC 3 cut(s) 200, 598, 1052
PsiI TTATAA 2 cut(s) 15, 896
Psp6I CCWGG 2 cut(s) 511, 790
PspFI CCCAGC 1 cut(s) 1036
PspGI CCWGG 2 cut(s) 511, 790
PspN4I GGNNCC 3 cut(s) 763, 908, 1117
PspPI GGNCC 2 cut(s) 907, 1010
PsuI RGATCY 2 cut(s) 258, 987
RsaI GTAC 3 cut(s) 461, 630, 774
RsaNI GTAC 3 cut(s) 460, 629, 773
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 5 cut(s) 86, 156, 429, 696, 1162
Sau3AI GATC 9 cut(s) 89, 258, 295, 308, 503, 537, 752, 987, 1125
Sau96I GGNCC 2 cut(s) 907, 1010
SchI GAGTC 3 cut(s) 200, 599, 1052
ScrFI CCNGG 2 cut(s) 513, 792
SduI GDGCHC 1 cut(s) 1037
SfaNI GCATC 4 cut(s) 556, 870, 1012, 1152
SinI GGWCC 2 cut(s) 907, 1010
SmlI CTYRAG 2 cut(s) 533, 914
SmoI CTYRAG 2 cut(s) 533, 914
SphI GCATGC 1 cut(s) 48
Sse9I AATT 5 cut(s) 17, 414, 486, 745, 1152
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 1 cut(s) 239
SspMI CTAG 2 cut(s) 365, 1140
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 2 cut(s) 511, 790
StyI CCWWGG 4 cut(s) 373, 594, 660, 901
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 31
TaqI TCGA 6 cut(s) 270, 300, 321, 490, 1128, 1146
TasI AATT 5 cut(s) 17, 414, 486, 745, 1152
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 2 cut(s) 230, 472
Tru1I TTAA 5 cut(s) 86, 156, 429, 696, 1162
Tru9I TTAA 5 cut(s) 86, 156, 429, 696, 1162
TscAI CASTG 4 cut(s) 566, 592, 654, 999
TspDTI ATGAA 6 cut(s) 53, 268, 318, 743, 995, 1005
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 4 cut(s) 566, 592, 654, 999
VpaK11BI GGWCC 2 cut(s) 907, 1010
XapI RAATTY 4 cut(s) 414, 486, 745, 1152
XceI RCATGY 1 cut(s) 48
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 2 cut(s) 365, 1140
Zsp2I ATGCAT 2 cut(s) 46, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.