pycom05g00460

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015543
Physical Location & Seq
Forward (+)
521571 .. 524130
2560 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00460.1

Sequence Viewer

Length: 1131 bp
ATGGCTAAAACAGCTCCGGTAGTTTTGACCCAAAACATTAATATTTCTGATACCCAAAGGGCCACCTCCTTTAATCATTCAACTGATTCCTCTAGCATCAACTTCCAAGAGCACTCCATTTCAGCTCCTGATGATGAAGTCCCCACCATTGATTACAGCATGATCTTCTCTGGTGATGTCGATCAACGACCCAAGGCCCTCCAATATCTTGCCTATGTTTGCGAGGAGTACGGCTTTTTTTATGTATGGAATTCCTCTCATCTACACACATTGTATCTGTATCTGATAAATCATGGTTTGCCGGATAGCGTAATTGACAAAGCACTGAAGGGAGTTTCCGAGTTCTTTAATCTGACGGAGGAGGAAAAGTTGGAGTATGAGAAGAAGGATTCGGCAGATAGGATCAGATGGGGGCTAGGTTTCTCACCTGGGGACCATGAAGCTGTCAAGCGGGAGTATCTCAAAGTTCTCCCACATCCAAAGTTTGAAGGGCCTGACAAACCAGCAGGTTTCAGCTTCGTAACTACTGGCCTTTCAACATTTTCTTTTACGTGTTACAGCAAGTCTTTGGAAGATTATTACCAAAGGGACCGAGAGGTGATGATAAACTTGGCAAAGGCAGTATCAAAAACGTTGGGATTCGAAGAAAATTACTTAGAGAAGGAACTAGGGTTGGAAATTGGCGCCGATGTTTCTGCAATGAACGTGTATCCCCCCTGGTTTAAATCCAACACTCCAATCGGTCTGCCGGCGCATCACGATCCTGGCTACCTAGTTTCCCTTGTACAAAACGTCAACGGCGGTCTCCAATTACACTATAAGCAAAAGTGGATCAATGTTCATATGCCTTCTAATTCCATTTTTGTTAATATTGGGGATCATCTTGAGGTTTTAACTAATGGGAAGTACAAGAGTCCTATGCATCGTGTGATCCTAAACAACAAGGTTACAAGAGTCTCTGTGGCCACAGTACACGGACCATCACACAACACATTCGTGAAACCAGCTCCAGAGTTTGTGGACGAGTCTCACCCGCCAAAATACCGAGGGATGATATACAAGGACTCCTTGGAAGCCAATGGTTACCATGAGATTGATGGAAAATCATGCCTACAACAACTTCGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

377

Amino Acids

42.58

Weight (kDa)

5.93

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 93 - 134 1.3e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 232 - 326 2.4e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015543)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15310 FvH4_2g15312
malus_domestica MD05G1008900.v1.1
prunus_persica Prupe.8G011000_v2.0.a1 Prupe.8G011200_v2.0.a1
pyrus_communis pycom05g00460
rosa_chinensis RchiOBHm_Chr6g0278051
rosa_laevigata RLG00000013242
rosa_multiflora Rmu_sc0000791.1_g000018
rosa_roxburghii Rroxscaffold_7G00190560
rosa_rugosa Rorug06G0114500
rosa_samantha Rh6AG224300 Rh6BG228300 Rh6CG230700 Rh6DG221800
rosa_wichuraiana Rw6G019580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 683
AciI CCGC 3 cut(s) 451, 801, 1034
AclI AACGTT 1 cut(s) 632
AclWI GGATC 5 cut(s) 410, 755, 839, 885, 925
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 4 cut(s) 230, 786, 908, 972
AflIII ACRYGT 2 cut(s) 551, 705
AgsI TTSAA 3 cut(s) 81, 488, 537
AjnI CCWGG 3 cut(s) 427, 716, 763
AleI CACNNNNGTG 1 cut(s) 995
AluBI AGCT 5 cut(s) 14, 125, 443, 516, 1007
AluI AGCT 5 cut(s) 14, 125, 443, 516, 1007
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 3 cut(s) 809, 961, 1032
AlwI GGATC 5 cut(s) 410, 755, 839, 885, 925
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 5 cut(s) 60, 195, 491, 529, 963
ApoI RAATTY 1 cut(s) 250
AseI ATTAAT 1 cut(s) 39
AspLEI GCGC 2 cut(s) 686, 754
AspS9I GGNCC 6 cut(s) 60, 196, 433, 491, 589, 977
AsuHPI GGTGA 4 cut(s) 185, 417, 610, 1022
AsuII TTCGAA 1 cut(s) 642
AvaII GGWCC 3 cut(s) 433, 589, 977
BalI TGGCCA 1 cut(s) 965
BanI GGYRCC 1 cut(s) 683
Bbv12I GWGCWC 1 cut(s) 114
BccI CCATC 3 cut(s) 402, 988, 1091
BceAI ACGGC 2 cut(s) 247, 814
BcgI CGANNNNNNTGC 2 cut(s) 677, 711
BciT130I CCWGG 3 cut(s) 429, 718, 765
BciVI GTATCC 1 cut(s) 720
BcoDI GTCTC 3 cut(s) 809, 961, 1032
BfaI CTAG 4 cut(s) 93, 416, 668, 773
BfoI RGCGCY 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 1 cut(s) 720
Bme1390I CCNGG 3 cut(s) 429, 718, 765
Bme18I GGWCC 3 cut(s) 433, 589, 977
BmgT120I GGNCC 6 cut(s) 60, 196, 433, 491, 589, 977
BmiI GGNNCC 3 cut(s) 434, 590, 685
BmrFI CCNGG 3 cut(s) 429, 718, 765
BmsI GCATC 3 cut(s) 105, 763, 931
BpmI CTGGAG 1 cut(s) 993
Bpu14I TTCGAA 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 905
BsaAI YACGTR 1 cut(s) 552
BsaBI GATNNNNATC 1 cut(s) 180
BsaHI GRCGYC 1 cut(s) 684
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 5 cut(s) 192, 428, 716, 1045, 1068
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 1049, 1079
Bse118I RCCGGY 1 cut(s) 748
Bse1I ACTGG 1 cut(s) 532
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 3 cut(s) 429, 718, 765
BseDI CCNNGG 5 cut(s) 192, 428, 716, 1045, 1068
BseGI GGATG 2 cut(s) 475, 1056
BseJI GATNNNNATC 1 cut(s) 180
BseMI GCAATG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 532
BseRI GAGGAG 2 cut(s) 239, 374
BshFI GGCC 5 cut(s) 62, 197, 493, 531, 965
BshNI GGYRCC 1 cut(s) 683
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 3 cut(s) 17, 302, 749
BslFI GGGAC 3 cut(s) 125, 446, 602
BsmAI GTCTC 3 cut(s) 809, 961, 1032
BsmFI GGGAC 3 cut(s) 125, 446, 602
BsnI GGCC 5 cut(s) 62, 197, 493, 531, 965
Bso31I GGTCTC 1 cut(s) 809
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 784
Bsp143I GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
BspACI CCGC 3 cut(s) 451, 801, 1034
BspANI GGCC 5 cut(s) 62, 197, 493, 531, 965
BspLI GGNNCC 3 cut(s) 434, 590, 685
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 5 cut(s) 410, 755, 839, 885, 925
BspT104I TTCGAA 1 cut(s) 642
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 748
BsrGI TGTACA 1 cut(s) 784
BsrI ACTGG 1 cut(s) 532
BssAI RCCGGY 1 cut(s) 748
BssECI CCNNGG 5 cut(s) 192, 428, 716, 1045, 1068
BssMI GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
BssNI GRCGYC 1 cut(s) 684
BssT1I CCWWGG 2 cut(s) 192, 1068
Bst2UI CCWGG 3 cut(s) 429, 718, 765
Bst4CI ACNGT 1 cut(s) 970
BstACI GRCGYC 1 cut(s) 684
BstAUI TGTACA 1 cut(s) 784
BstBAI YACGTR 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 1 cut(s) 655
BstEII GGTNACC 1 cut(s) 1082
BstF5I GGATG 2 cut(s) 475, 1056
BstH2I RGCGCY 1 cut(s) 687
BstHHI GCGC 2 cut(s) 686, 754
BstKTI GATC 7 cut(s) 165, 184, 405, 763, 834, 880, 933
BstMAI GTCTC 3 cut(s) 809, 961, 1032
BstMBI GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 3 cut(s) 429, 718, 765
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 3 cut(s) 427, 716, 763
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 5 cut(s) 62, 197, 493, 531, 965
BtsCI GGATG 2 cut(s) 475, 1056
BtsIMutI CAGTG 1 cut(s) 323
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 750
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 2 cut(s) 686, 754
Cfr10I RCCGGY 1 cut(s) 748
Cfr13I GGNCC 6 cut(s) 60, 196, 433, 491, 589, 977
Csp6I GTAC 4 cut(s) 229, 785, 907, 971
CviAII CATG 5 cut(s) 160, 293, 437, 1088, 1107
CviQI GTAC 4 cut(s) 229, 785, 907, 971
DdeI CTNAG 1 cut(s) 655
DinI GGCGCC 1 cut(s) 685
DpnI GATC 7 cut(s) 164, 183, 404, 762, 833, 879, 932
DpnII GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
DraI TTTAAA 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 963
Eco130I CCWWGG 2 cut(s) 192, 1068
Eco31I GGTCTC 1 cut(s) 809
Eco47I GGWCC 3 cut(s) 433, 589, 977
Eco57I CTGAAG 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 1082
EcoO109I RGGNCCY 2 cut(s) 196, 491
EcoO65I GGTNACC 1 cut(s) 1082
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 3 cut(s) 427, 716, 763
EcoT14I CCWWGG 2 cut(s) 192, 1068
EcoT22I ATGCAT 1 cut(s) 924
EgeI GGCGCC 1 cut(s) 685
EheI GGCGCC 1 cut(s) 685
ErhI CCWWGG 2 cut(s) 192, 1068
FaeI CATG 5 cut(s) 163, 296, 440, 1091, 1110
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 3 cut(s) 125, 446, 602
FatI CATG 5 cut(s) 159, 292, 436, 1087, 1106
FauI CCCGC 2 cut(s) 444, 1041
FauNDI CATATG 1 cut(s) 843
FokI GGATG 2 cut(s) 462, 1063
FspBI CTAG 4 cut(s) 93, 416, 668, 773
GlaI GCGC 2 cut(s) 685, 753
GsuI CTGGAG 1 cut(s) 993
HaeII RGCGCY 1 cut(s) 687
HaeIII GGCC 5 cut(s) 62, 197, 493, 531, 965
HapII CCGG 3 cut(s) 17, 302, 749
HhaI GCGC 2 cut(s) 686, 754
Hin1I GRCGYC 1 cut(s) 684
Hin1II CATG 5 cut(s) 163, 296, 440, 1091, 1110
Hin6I GCGC 2 cut(s) 684, 752
HinP1I GCGC 2 cut(s) 684, 752
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 7 cut(s) 86, 389, 639, 913, 954, 1025, 1064
HpaII CCGG 3 cut(s) 17, 302, 749
HphI GGTGA 4 cut(s) 185, 417, 610, 1022
Hpy166II GTNNAC 3 cut(s) 796, 973, 1021
Hpy188I TCNGA 6 cut(s) 49, 285, 340, 354, 407, 1125
Hpy188III TCNNGA 5 cut(s) 128, 758, 884, 997, 1010
Hpy8I GTNNAC 3 cut(s) 796, 973, 1021
HpyAV CCTTC 5 cut(s) 322, 379, 482, 655, 858
HpyCH4III ACNGT 1 cut(s) 970
HpyCH4IV ACGT 4 cut(s) 551, 632, 705, 792
HpyCH4V TGCA 2 cut(s) 698, 922
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 655
HpySE526I ACGT 4 cut(s) 551, 632, 705, 792
Hsp92I GRCGYC 1 cut(s) 684
Hsp92II CATG 5 cut(s) 163, 296, 440, 1091, 1110
HspAI GCGC 2 cut(s) 684, 752
KasI GGCGCC 1 cut(s) 683
KroI GCCGGC 1 cut(s) 748
KroNI GCCGGC 1 cut(s) 750
Kzo9I GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
LmnI GCTCC 3 cut(s) 19, 130, 1012
LweI GCATC 3 cut(s) 105, 763, 931
MaeI CTAG 4 cut(s) 93, 416, 668, 773
MaeII ACGT 4 cut(s) 551, 632, 705, 792
MaeIII GTNAC 4 cut(s) 520, 554, 946, 1082
MalI GATC 7 cut(s) 164, 183, 404, 762, 833, 879, 932
MboI GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
MboII GAAGA 4 cut(s) 157, 394, 584, 656
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 965
MluCI AATT 6 cut(s) 250, 312, 649, 678, 809, 853
MluNI TGGCCA 1 cut(s) 965
Mly113I GGCGCC 1 cut(s) 684
MlyI GAGTC 4 cut(s) 922, 963, 1034, 1058
MmeI TCCRAC 3 cut(s) 351, 654, 753
Mox20I TGGCCA 1 cut(s) 965
Mph1103I ATGCAT 1 cut(s) 924
MroNI GCCGGC 1 cut(s) 748
MscI TGGCCA 1 cut(s) 965
MseI TTAA 6 cut(s) 39, 72, 348, 723, 867, 893
MslI CAYNNNNRTG 1 cut(s) 995
Msp20I TGGCCA 1 cut(s) 965
MspI CCGG 3 cut(s) 17, 302, 749
MspR9I CCNGG 3 cut(s) 429, 718, 765
MvaI CCWGG 3 cut(s) 429, 718, 765
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 750
NarI GGCGCC 1 cut(s) 684
NdeI CATATG 1 cut(s) 843
NdeII GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
NgoMIV GCCGGC 1 cut(s) 748
NlaIII CATG 5 cut(s) 163, 296, 440, 1091, 1110
NlaIV GGNNCC 3 cut(s) 434, 590, 685
NsiI ATGCAT 1 cut(s) 924
NspV TTCGAA 1 cut(s) 642
OliI CACNNNNGTG 1 cut(s) 995
PdiI GCCGGC 1 cut(s) 750
PfeI GAWTC 3 cut(s) 86, 389, 639
PleI GAGTC 4 cut(s) 921, 962, 1033, 1058
PluTI GGCGCC 1 cut(s) 687
PpsI GAGTC 4 cut(s) 921, 962, 1033, 1058
Ppu21I YACGTR 1 cut(s) 552
PshBI ATTAAT 1 cut(s) 39
Psp1406I AACGTT 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 427, 716, 763
PspEI GGTNACC 1 cut(s) 1082
PspGI CCWGG 3 cut(s) 427, 716, 763
PspN4I GGNNCC 3 cut(s) 434, 590, 685
PspPI GGNCC 6 cut(s) 60, 196, 433, 491, 589, 977
PstNI CAGNNNCTG 1 cut(s) 128
RsaI GTAC 4 cut(s) 230, 786, 908, 972
RsaNI GTAC 4 cut(s) 229, 785, 907, 971
RseI CAYNNNNRTG 1 cut(s) 995
SaqAI TTAA 6 cut(s) 39, 72, 348, 723, 867, 893
Sau3AI GATC 7 cut(s) 162, 181, 402, 760, 831, 877, 930
Sau96I GGNCC 6 cut(s) 60, 196, 433, 491, 589, 977
SchI GAGTC 4 cut(s) 922, 963, 1034, 1058
ScrFI CCNGG 3 cut(s) 429, 718, 765
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 3 cut(s) 105, 763, 931
SfoI GGCGCC 1 cut(s) 685
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 3 cut(s) 433, 589, 977
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
Sse9I AATT 6 cut(s) 250, 312, 649, 678, 809, 853
SsiI CCGC 3 cut(s) 451, 801, 1034
SspDI GGCGCC 1 cut(s) 683
SspI AATATT 2 cut(s) 43, 871
SspMI CTAG 4 cut(s) 93, 416, 668, 773
StyD4I CCNGG 3 cut(s) 427, 716, 763
StyI CCWWGG 2 cut(s) 192, 1068
TaaI ACNGT 1 cut(s) 970
TaiI ACGT 4 cut(s) 554, 635, 708, 795
TaqI TCGA 2 cut(s) 180, 642
TaqII GACCGA 2 cut(s) 606, 731
TasI AATT 6 cut(s) 250, 312, 649, 678, 809, 853
TatI WGTACW 3 cut(s) 784, 906, 970
TfiI GAWTC 3 cut(s) 86, 389, 639
Tru1I TTAA 6 cut(s) 39, 72, 348, 723, 867, 893
Tru9I TTAA 6 cut(s) 39, 72, 348, 723, 867, 893
TscAI CASTG 1 cut(s) 330
TspDTI ATGAA 4 cut(s) 150, 453, 716, 830
TspGWI ACGGA 2 cut(s) 371, 990
TspRI CASTG 1 cut(s) 330
VpaK11BI GGWCC 3 cut(s) 433, 589, 977
VspI ATTAAT 1 cut(s) 39
XapI RAATTY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 1094
XspI CTAG 4 cut(s) 93, 416, 668, 773
Zsp2I ATGCAT 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.