MD05G1016200.v1.1

Belongs to the glycosyltransferase 8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008607
Physical Location & Seq
Reverse (-)
2875268 .. 2878212
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1016200.v1.1.491

Sequence Viewer

Length: 1647 bp
ATGAAAAGGAGCGTTCCAGCAGATTTACGTTATATATCATCTTTACACATAAACTGGAGTGAAATTTTGGACTTCGTTGGAAAACTTACTCACAAAAATGATTATCGGAGAATTGGCCTATTAAACTTTAATGACAATGAAATTGACAATTGGAAGGAGCTCTTTCCAGATTCTGAGCAGGTTGTTCTGCATCTCAACCATGCACCAAACAACATAACATGGGAATCCCTCTATCCTGAATGGATAGACGAAGAACAAGAATCCAAAGTTCCTACTTGTCCCTCTCTACCAAAGCTTCTAATTCCTGAAAGTTCACCACTTGGTCTGGTTGCTGTCAAGCTTCCCTGCAACAAGTCAGGCAGTTGGTCAAGGGACGTGGCTCGTTTCCACTTGCAGCTTGAAGCAGCAAGGCTGGCTGCGTTATCGGAAGGATATCACCCAGTGCATGTGCTTTTGTTGACTGACTGCTTCCCCATTCCAAGTTTATTCACTTGCAAAGATCTTGTTAGACGTGAAGGGAATGCATGGCTCTATGAACCGAAACTTACTACCTTGAGGGAGAAGCTACAGCTCCCTGTAGGATCATGTGAGCTTGCAGTTCCTCTCAAGGCTAAAGAGCATTTTGACTCGGAAAAACCCCACCGAGAAGCATATGCAACAATCCTACACTCTGCAGATGTATATGTTTGTGGAGCCATTGCTGCAGCGCAGAGCATCCGTATGGCTGGTTCAACACGAGATCTTGTGATACTGGTCGATGAAACAATCACTCAGTACCATCGGAGAGGACTGGAGGCTGCTGGGTGGAAGATCCACACAATCCAAAGAATCAGGAACCCAAAAGCTGAACCAGAAGCTTACAACGAATGGAACTACAGCAAATTCCGTCTTTGGCAGTTGACTGATTACGACAAGATCATTTTCATTGATGCCGACATGCTTATACTTCGGAATATCGATTTCCTGTTTCAAATGCCAGAAATATCAGCCACGGGAAACAATGCTACTTTGTTCAACTCAGGTGTGATGGTCGTCGAACCATCAAATTGTACATTCCAGCTGCTCATGGATCATATCAACGAAATTGTATCCTACAACGGTGGTGACCAGGGGTATCTGAATGAGGTATTTACATGGTGGCACAGGATTCCGAAACACATGAACTTCTTGAAACATTTCTGGGAAGGTGATGAGCCGGAGATAAAGCAAATGAAAACTCACCTCTTTCAAGCTGACCCTCCAATCATCTATGTCCTCCATTACCTTGGTAAGAAACCGTGGCTTTGCTTCCGCGACTATGACTGCAATTGGAATGTCGATTTTATGCAGGAGTTTGCGAGTGATGTTGCACATAATACGTGGTGGAGAGTGCACGATACTATGCCAGGAAACTTGCAAAAGTTCTGTTTGCTTCGGACACAGCAAAAGGCAGGACTAGAGTGGGACCGAAGGCAAGCGGAAAAAGCAAATTACACCGATGGTCACTGGAAAATTAAAATAAAAGACAAGCGTCTTAAGATATGCGTCCAGGATTTCTGCTCCTGGAAGAGCATGTTACGGCACTGGGGTGAAAAAAGTTGGACAGATAACGCTACTGTTACTCCCTCACTACCTGCACTTACTACTTCATCGGTCACTTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_8

Protein Analysis

549

Amino Acids

63.67

Weight (kDa)

6.35

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_8 PF01501 222 - 428 4.3e-13 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 169, 1621
AccII CGCG 1 cut(s) 1293
AciI CCGC 2 cut(s) 1291, 1457
AclWI GGATC 3 cut(s) 589, 805, 1077
AcsI RAATTY 2 cut(s) 63, 881
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 2 cut(s) 776, 1051
AflII CTTAAG 1 cut(s) 1514
AgsI TTSAA 6 cut(s) 401, 732, 971, 1015, 1171, 1229
AjiI CACGTC 2 cut(s) 376, 512
AjnI CCWGG 4 cut(s) 1107, 1384, 1527, 1541
AleI CACNNNNGTG 1 cut(s) 1566
Alw21I GWGCWC 2 cut(s) 162, 1374
Alw44I GTGCAC 1 cut(s) 1370
AlwI GGATC 3 cut(s) 589, 805, 1077
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 1370
ApeKI GCWGC 7 cut(s) 394, 404, 416, 701, 704, 797, 1060
ApoI RAATTY 2 cut(s) 63, 881
Asp700I GAANNNNTTC 1 cut(s) 1175
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 1 cut(s) 1444
AsuHPI GGTGA 6 cut(s) 306, 428, 1115, 1199, 1211, 1580
AvaII GGWCC 1 cut(s) 1444
BaeGI GKGCMC 1 cut(s) 1374
BanII GRGCYC 1 cut(s) 162
BauI CACGAG 1 cut(s) 735
Bbv12I GWGCWC 2 cut(s) 162, 1374
BbvI GCAGC 7 cut(s) 403, 406, 416, 688, 716, 784, 1047
BccI CCATC 4 cut(s) 786, 1021, 1048, 1472
BceAI ACGGC 1 cut(s) 1574
BciT130I CCWGG 4 cut(s) 1109, 1386, 1529, 1543
BciVI GTATCC 1 cut(s) 1099
BfaI CTAG 1 cut(s) 1436
BfmI CTRYAG 5 cut(s) 566, 576, 672, 702, 874
BfrI CTTAAG 1 cut(s) 1514
BfuAI ACCTGC 2 cut(s) 169, 1621
BfuI GTATCC 1 cut(s) 1099
BglII AGATCT 2 cut(s) 499, 739
BisI GCNGC 7 cut(s) 395, 405, 417, 702, 705, 798, 1061
BlsI GCNGC 7 cut(s) 396, 406, 418, 703, 706, 799, 1062
Bme1390I CCNGG 4 cut(s) 1109, 1386, 1529, 1543
Bme18I GGWCC 1 cut(s) 1444
BmgBI CACGTC 2 cut(s) 376, 512
BmgT120I GGNCC 1 cut(s) 1444
BmiI GGNNCC 3 cut(s) 694, 836, 1445
BmrFI CCNGG 4 cut(s) 1109, 1386, 1529, 1543
BmrI ACTGGG 2 cut(s) 434, 1573
BmsI GCATC 3 cut(s) 199, 723, 919
BmuI ACTGGG 2 cut(s) 434, 1573
BoxI GACNNNNGTC 1 cut(s) 1509
BpmI CTGGAG 2 cut(s) 76, 812
BpuEI CTTGAG 2 cut(s) 574, 590
Bsa29I ATCGAT 1 cut(s) 957
BsaAI YACGTR 1 cut(s) 1359
BsaJI CCNNGG 4 cut(s) 990, 1108, 1264, 1277
BsaXI ACNNNNNCTCC 2 cut(s) 1585, 1615
Bse1I ACTGG 6 cut(s) 59, 440, 756, 795, 1490, 1568
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 4 cut(s) 1109, 1386, 1529, 1543
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 4 cut(s) 990, 1108, 1264, 1277
BseGI GGATG 1 cut(s) 714
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 3 cut(s) 165, 785, 1032
BseNI ACTGG 6 cut(s) 59, 440, 756, 795, 1490, 1568
BseSI GKGCMC 1 cut(s) 1374
BseXI GCAGC 7 cut(s) 403, 406, 416, 688, 716, 784, 1047
BseYI CCCAGC 1 cut(s) 800
BsgI GTGCAG 1 cut(s) 1599
Bsh1236I CGCG 1 cut(s) 1293
BshFI GGCC 1 cut(s) 117
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 2 cut(s) 162, 1374
BsiSI CCGG 1 cut(s) 1196
BslFI GGGAC 3 cut(s) 264, 386, 1457
BsmFI GGGAC 3 cut(s) 264, 386, 1457
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 162, 1374
Bsp1407I TGTACA 1 cut(s) 1049
Bsp143I GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
BspACI CCGC 2 cut(s) 1291, 1457
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 3 cut(s) 166, 784, 1031
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 1 cut(s) 1293
BspLI GGNNCC 3 cut(s) 694, 836, 1445
BspMAI CTGCAG 2 cut(s) 676, 706
BspMI ACCTGC 2 cut(s) 169, 1621
BspPI GGATC 3 cut(s) 589, 805, 1077
BspQI GCTCTTC 1 cut(s) 1541
BspTI CTTAAG 1 cut(s) 1514
BsrDI GCAATG 1 cut(s) 696
BsrGI TGTACA 1 cut(s) 1049
BsrI ACTGG 6 cut(s) 59, 440, 756, 795, 1490, 1568
BssECI CCNNGG 4 cut(s) 990, 1108, 1264, 1277
BssMI GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
BssSI CACGAG 1 cut(s) 735
BssT1I CCWWGG 1 cut(s) 1264
Bst2BI CACGAG 1 cut(s) 735
Bst2UI CCWGG 4 cut(s) 1109, 1386, 1529, 1543
Bst4CI ACNGT 3 cut(s) 1100, 1278, 1597
Bst6I CTCTTC 1 cut(s) 1541
BstAFI CTTAAG 1 cut(s) 1514
BstAUI TGTACA 1 cut(s) 1049
BstBAI YACGTR 1 cut(s) 1359
BstC8I GCNNGC 3 cut(s) 414, 594, 1455
BstDEI CTNAG 3 cut(s) 174, 771, 1018
BstDSI CCRYGG 2 cut(s) 990, 1277
BstEII GGTNACC 1 cut(s) 1103
BstF5I GGATG 1 cut(s) 714
BstFNI CGCG 1 cut(s) 1293
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 6 cut(s) 502, 584, 742, 813, 918, 1072
BstMBI GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 413, 701, 1463
BstNI CCWGG 4 cut(s) 1109, 1386, 1529, 1543
BstNSI RCATGY 3 cut(s) 449, 940, 1555
BstPAI GACNNNNGTC 1 cut(s) 1509
BstPI GGTNACC 1 cut(s) 1103
BstSCI CCNGG 4 cut(s) 1107, 1384, 1527, 1541
BstSFI CTRYAG 5 cut(s) 566, 576, 672, 702, 874
BstSLI GKGCMC 1 cut(s) 1374
BstUI CGCG 1 cut(s) 1293
BstV1I GCAGC 7 cut(s) 403, 406, 416, 688, 716, 784, 1047
BstX2I RGATCY 3 cut(s) 499, 739, 810
BstXI CCANNNNNNTGG 1 cut(s) 1265
BstYI RGATCY 3 cut(s) 499, 739, 810
Bsu15I ATCGAT 1 cut(s) 957
BsuI GTATCC 1 cut(s) 1099
BsuRI GGCC 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 2 cut(s) 990, 1277
BtrI CACGTC 2 cut(s) 376, 512
BtsCI GGATG 1 cut(s) 714
BtsIMutI CAGTG 3 cut(s) 447, 1483, 1561
BveI ACCTGC 2 cut(s) 169, 1621
Cac8I GCNNGC 3 cut(s) 414, 594, 1455
CaiI CAGNNNCTG 1 cut(s) 173
CfoI GCGC 1 cut(s) 709
Cfr13I GGNCC 1 cut(s) 1444
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 2 cut(s) 1499, 1513
Csp6I GTAC 2 cut(s) 775, 1050
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CviQI GTAC 2 cut(s) 775, 1050
DdeI CTNAG 3 cut(s) 174, 771, 1018
DpnI GATC 6 cut(s) 501, 583, 741, 812, 917, 1071
DpnII GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
DraIII CACNNNGTG 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 1541
EarI CTCTTC 1 cut(s) 1541
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 1264
Eco24I GRGCYC 1 cut(s) 162
Eco32I GATATC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 1444
Eco53kI GAGCTC 1 cut(s) 160
Eco91I GGTNACC 1 cut(s) 1103
EcoICRI GAGCTC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 1103
EcoRII CCWGG 4 cut(s) 1107, 1384, 1527, 1541
EcoRV GATATC 1 cut(s) 434
EcoT14I CCWWGG 1 cut(s) 1264
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 1264
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 3 cut(s) 264, 386, 1457
FauNDI CATATG 1 cut(s) 652
Fnu4HI GCNGC 7 cut(s) 395, 405, 417, 702, 705, 798, 1061
FokI GGATG 1 cut(s) 701
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 7 cut(s) 395, 405, 417, 702, 705, 798, 1061
FspBI CTAG 1 cut(s) 1436
GlaI GCGC 1 cut(s) 708
GluI GCNGC 7 cut(s) 395, 405, 417, 702, 705, 798, 1061
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 2 cut(s) 76, 812
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 1196
HgaI GACGC 2 cut(s) 1499, 1513
HhaI GCGC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HincII GTYRAC 2 cut(s) 459, 900
HindII GTYRAC 2 cut(s) 459, 900
HindIII AAGCTT 3 cut(s) 293, 338, 855
HinfI GANTC 6 cut(s) 170, 224, 260, 626, 828, 1147
HpaII CCGG 1 cut(s) 1196
HphI GGTGA 6 cut(s) 306, 428, 1115, 1199, 1211, 1580
Hpy166II GTNNAC 4 cut(s) 314, 459, 900, 1372
Hpy188I TCNGA 9 cut(s) 108, 175, 427, 631, 783, 951, 1119, 1152, 1416
Hpy188III TCNNGA 6 cut(s) 167, 236, 305, 832, 1168, 1644
Hpy8I GTNNAC 4 cut(s) 314, 459, 900, 1372
Hpy99I CGWCG 1 cut(s) 1037
HpyAV CCTTC 5 cut(s) 148, 422, 509, 1178, 1443
HpyCH4III ACNGT 3 cut(s) 1100, 1278, 1597
HpyCH4IV ACGT 4 cut(s) 28, 375, 511, 1358
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 701, 1463
HpyF3I CTNAG 3 cut(s) 174, 771, 1018
HpySE526I ACGT 4 cut(s) 28, 375, 511, 1358
HspAI GCGC 1 cut(s) 707
Kzo9I GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
LguI GCTCTTC 1 cut(s) 1541
LmnI GCTCC 5 cut(s) 9, 157, 576, 692, 1544
Lsp1109I GCAGC 7 cut(s) 403, 406, 416, 688, 716, 784, 1047
LweI GCATC 3 cut(s) 199, 723, 919
MaeI CTAG 1 cut(s) 1436
MaeII ACGT 4 cut(s) 28, 375, 511, 1358
MaeIII GTNAC 5 cut(s) 1103, 1481, 1554, 1597, 1633
MalI GATC 6 cut(s) 501, 583, 741, 812, 917, 1071
MboI GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
MboII GAAGA 3 cut(s) 263, 820, 1558
MfeI CAATTG 2 cut(s) 148, 1306
MflI RGATCY 3 cut(s) 499, 739, 810
MhlI GDGCHC 2 cut(s) 162, 1374
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 2 cut(s) 58, 1559
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 1175
MseI TTAA 4 cut(s) 122, 129, 1494, 1515
MslI CAYNNNNRTG 3 cut(s) 96, 719, 1566
MspA1I CMGCKG 1 cut(s) 1060
MspCI CTTAAG 1 cut(s) 1514
MspI CCGG 1 cut(s) 1196
MspR9I CCNGG 4 cut(s) 1109, 1386, 1529, 1543
MunI CAATTG 2 cut(s) 148, 1306
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 4 cut(s) 1109, 1386, 1529, 1543
MvnI CGCG 1 cut(s) 1293
MwoI GCNNNNNNNGC 3 cut(s) 413, 701, 1463
NdeI CATATG 1 cut(s) 652
NdeII GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
NlaIV GGNNCC 3 cut(s) 694, 836, 1445
NmuCI GTSAC 3 cut(s) 1103, 1481, 1633
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 3 cut(s) 449, 940, 1555
OliI CACNNNNGTG 1 cut(s) 1566
PciSI GCTCTTC 1 cut(s) 1541
PctI GAATGC 1 cut(s) 526
PdmI GAANNNNTTC 1 cut(s) 1175
PfeI GAWTC 5 cut(s) 170, 224, 260, 828, 1147
PfoI TCCNGGA 2 cut(s) 1527, 1541
PkrI GCNGC 7 cut(s) 396, 406, 418, 703, 706, 799, 1062
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
Ppu21I YACGTR 1 cut(s) 1359
PshAI GACNNNNGTC 1 cut(s) 1509
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 4 cut(s) 1107, 1384, 1527, 1541
PspEI GGTNACC 1 cut(s) 1103
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 4 cut(s) 1107, 1384, 1527, 1541
PspN4I GGNNCC 3 cut(s) 694, 836, 1445
PspPI GGNCC 1 cut(s) 1444
PsrI GAACNNNNNNTAC 2 cut(s) 712, 744
PstI CTGCAG 2 cut(s) 676, 706
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 3 cut(s) 499, 739, 810
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 2 cut(s) 776, 1051
RsaNI GTAC 2 cut(s) 775, 1050
RseI CAYNNNNRTG 3 cut(s) 96, 719, 1566
SacI GAGCTC 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 1541
SaqAI TTAA 4 cut(s) 122, 129, 1494, 1515
SatI GCNGC 7 cut(s) 395, 405, 417, 702, 705, 798, 1061
Sau3AI GATC 6 cut(s) 499, 581, 739, 810, 915, 1069
Sau96I GGNCC 1 cut(s) 1444
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 4 cut(s) 1109, 1386, 1529, 1543
SduI GDGCHC 2 cut(s) 162, 1374
SfaNI GCATC 3 cut(s) 199, 723, 919
SfcI CTRYAG 5 cut(s) 566, 576, 672, 702, 874
SinI GGWCC 1 cut(s) 1444
SmiMI CAYNNNNRTG 3 cut(s) 96, 719, 1566
SmlI CTYRAG 3 cut(s) 553, 605, 1514
SmoI CTYRAG 3 cut(s) 553, 605, 1514
SsiI CCGC 2 cut(s) 1291, 1457
SspMI CTAG 1 cut(s) 1436
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 4 cut(s) 1107, 1384, 1527, 1541
StyI CCWWGG 1 cut(s) 1264
TaaI ACNGT 3 cut(s) 1100, 1278, 1597
TaiI ACGT 4 cut(s) 31, 378, 514, 1361
TaqI TCGA 4 cut(s) 756, 957, 1035, 1317
TaqII GACCGA 2 cut(s) 1461, 1621
TatI WGTACW 1 cut(s) 1049
TfiI GAWTC 5 cut(s) 170, 224, 260, 828, 1147
Tru1I TTAA 4 cut(s) 122, 129, 1494, 1515
Tru9I TTAA 4 cut(s) 122, 129, 1494, 1515
TscAI CASTG 3 cut(s) 447, 1490, 1568
TseFI GTSAC 3 cut(s) 1103, 1481, 1633
TseI GCWGC 7 cut(s) 394, 404, 416, 701, 704, 797, 1060
Tsp45I GTSAC 3 cut(s) 1103, 1481, 1633
TspDTI ATGAA 8 cut(s) 17, 153, 549, 774, 913, 1175, 1226, 1617
TspGWI ACGGA 2 cut(s) 707, 875
TspRI CASTG 3 cut(s) 447, 1490, 1568
Vha464I CTTAAG 1 cut(s) 1514
VneI GTGCAC 1 cut(s) 1370
VpaK11BI GGWCC 1 cut(s) 1444
XapI RAATTY 2 cut(s) 63, 881
XceI RCATGY 3 cut(s) 449, 940, 1555
XmnI GAANNNNTTC 1 cut(s) 1175
XspI CTAG 1 cut(s) 1436
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.