MD05G1028800.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0018880
Physical Location & Seq
Reverse (-)
4695102 .. 4698782
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1028800.v1.1.491

Sequence Viewer

Length: 825 bp
ATGATAGCATTTCTTTTCATTTCGAAGTTTAGCAGTTTTCTTTCTAATATTAGAGAGGTTTATAAATGTGGTAATCTAGTTGATCTACAAAGACAGATTCTTTTTCCGATCTTGAGGGAACAAATTACCCAAGTATGGGATGAACAGTGTGGAACCTCTTTCATTAAGACTTGCTTCCATAATAAAAAGGTTCTTCTCATTCTTGATGATGTGGATGAATCAAGACAATTTGAAGTATTGGTTGGAAAGAGGGATTGGTTTGGTAAGGGGAGTAGAATCATCATTACAACTAGAGACCCTGACAAAGGTTTTCTGGAATCGTCCGAGTGTTTTGTAAATTATGCCCAAGGCCTTCCACTAGCTCTTAAAATTTTGGGATGTTCTTTGTATAAGAGAGATCAAGATGAATGGAAAAGTGAATTAGAAAAGCTACAGAAAATTCCTAAGTCAACAATTTTTGATGCACTCAAAATAAGTTATGATGGACTAGATGAGATGAACAAGAGAATATTTCTGGATATTGCACATTTCCATAAGGGGAAGGACAAAGAGGAAGTAATTGAAATACTAGACACCTATTGCCTTTATGGTCTGATCGGGGTAGATGCTCTCATCCAGAAGTCCTTATTAACTATGGAGAAATCCCAGTTTCATATTTCATACATCAATGTGGAGATGCATGATTTGGTACAACAAATGGCACTGGAAATTGTTCATCAAAAGTCTTGTACTGGAGAGCCTGGTGAACGTGTTCATTTGTGTCATCCTGAGGACATCTTTCAAGTGTTCATTAACAACACGGGAACGAGCCAAATTGAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

275

Amino Acids

31.67

Weight (kDa)

5.54

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 165 - 242 1.3e-10 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018880)

Species Orthologous Gene IDs
malus_domestica MD05G1028800.v1.1 MD10G1018600.v1.1
pyrus_communis pycom04g04790
rosa_laevigata RLG00000030175 RLG00000030179 RLG00000030182
rosa_samantha Rh1CG065500 Rh1DG070600
rosa_wichuraiana Rw1G005370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
AcsI RAATTY 2 cut(s) 369, 438
AfaI GTAC 2 cut(s) 690, 730
AfiI CCNNNNNNNGG 4 cut(s) 135, 136, 305, 817
AflIII ACRYGT 1 cut(s) 748
AgsI TTSAA 3 cut(s) 233, 563, 782
AjnI CCWGG 1 cut(s) 739
AjuI GAANNNNNNNTTGG 4 cut(s) 225, 238, 257, 270
AluBI AGCT 2 cut(s) 362, 430
AluI AGCT 2 cut(s) 362, 430
Alw26I GTCTC 1 cut(s) 288
AoxI GGCC 1 cut(s) 349
ApoI RAATTY 2 cut(s) 369, 438
Asp700I GAANNNNTTC 2 cut(s) 711, 750
AsuHPI GGTGA 1 cut(s) 755
AsuII TTCGAA 1 cut(s) 23
AxyI CCTNAGG 1 cut(s) 768
BccI CCATC 1 cut(s) 476
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 5 cut(s) 77, 291, 359, 488, 569
BfmI CTRYAG 1 cut(s) 431
Bme1390I CCNGG 1 cut(s) 741
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 741
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 3 cut(s) 451, 595, 666
BmuI ACTGGG 1 cut(s) 640
BpmI CTGGAG 1 cut(s) 753
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 346
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 136, 305, 817
Bse1I ACTGG 3 cut(s) 646, 708, 736
Bse21I CCTNAGG 1 cut(s) 768
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 5 cut(s) 145, 220, 383, 612, 763
BseLI CCNNNNNNNGG 4 cut(s) 135, 136, 305, 817
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 3 cut(s) 646, 708, 736
BshFI GGCC 1 cut(s) 351
BslI CCNNNNNNNGG 4 cut(s) 135, 136, 305, 817
BsmAI GTCTC 1 cut(s) 288
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 1 cut(s) 288
Bsp119I TTCGAA 1 cut(s) 23
Bsp143I GATC 4 cut(s) 82, 108, 397, 594
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 760
BspLI GGNNCC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 288
BsrI ACTGG 3 cut(s) 646, 708, 736
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 4 cut(s) 82, 108, 397, 594
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 444, 768
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 5 cut(s) 145, 220, 383, 612, 763
BstKTI GATC 4 cut(s) 85, 111, 400, 597
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 82, 108, 397, 594
BstNI CCWGG 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 431
Bsu36I CCTNAGG 1 cut(s) 768
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 5 cut(s) 145, 220, 383, 612, 763
BtsIMutI CAGTG 2 cut(s) 152, 701
Csp6I GTAC 2 cut(s) 689, 729
CviAII CATG 1 cut(s) 680
CviJI RGCY 5 cut(s) 351, 362, 430, 739, 810
CviKI_1 RGCY 5 cut(s) 351, 362, 430, 739, 810
CviQI GTAC 2 cut(s) 689, 729
DdeI CTNAG 2 cut(s) 444, 768
DpnI GATC 4 cut(s) 84, 110, 399, 596
DpnII GATC 4 cut(s) 82, 108, 397, 594
Eco130I CCWWGG 1 cut(s) 346
Eco147I AGGCCT 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 288
Eco81I CCTNAGG 1 cut(s) 768
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 1 cut(s) 683
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 1 cut(s) 679
FokI GGATG 5 cut(s) 152, 227, 390, 599, 750
FspBI CTAG 5 cut(s) 77, 291, 359, 488, 569
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 1 cut(s) 351
Hin1II CATG 1 cut(s) 683
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 4 cut(s) 97, 218, 276, 317
HphI GGTGA 1 cut(s) 755
Hpy166II GTNNAC 2 cut(s) 450, 746
Hpy188I TCNGA 3 cut(s) 108, 325, 594
Hpy188III TCNNGA 8 cut(s) 112, 203, 222, 314, 401, 515, 616, 767
Hpy8I GTNNAC 2 cut(s) 450, 746
HpyAV CCTTC 2 cut(s) 362, 535
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 1 cut(s) 748
HpyCH4V TGCA 3 cut(s) 464, 524, 679
HpyF3I CTNAG 2 cut(s) 444, 768
HpySE526I ACGT 1 cut(s) 748
Hsp92II CATG 1 cut(s) 683
Kzo9I GATC 4 cut(s) 82, 108, 397, 594
LweI GCATC 3 cut(s) 451, 595, 666
MaeI CTAG 5 cut(s) 77, 291, 359, 488, 569
MaeII ACGT 1 cut(s) 748
MalI GATC 4 cut(s) 84, 110, 399, 596
MboI GATC 4 cut(s) 82, 108, 397, 594
MboII GAAGA 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 7 cut(s) 49, 108, 166, 243, 544, 763, 811
Mph1103I ATGCAT 1 cut(s) 681
MroXI GAANNNNTTC 2 cut(s) 711, 750
MseI TTAA 4 cut(s) 165, 366, 629, 792
MslI CAYNNNNRTG 1 cut(s) 668
MspR9I CCNGG 1 cut(s) 741
MvaI CCWGG 1 cut(s) 741
NdeII GATC 4 cut(s) 82, 108, 397, 594
NlaIII CATG 1 cut(s) 683
NlaIV GGNNCC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 681
NspV TTCGAA 1 cut(s) 23
PceI AGGCCT 1 cut(s) 351
PdmI GAANNNNTTC 2 cut(s) 711, 750
PfeI GAWTC 4 cut(s) 97, 218, 276, 317
PsiI TTATAA 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 1 cut(s) 154
RsaI GTAC 2 cut(s) 690, 730
RsaNI GTAC 2 cut(s) 689, 729
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 4 cut(s) 165, 366, 629, 792
Sau3AI GATC 4 cut(s) 82, 108, 397, 594
ScrFI CCNGG 1 cut(s) 741
SetI ASST 8 cut(s) 60, 158, 192, 310, 364, 432, 578, 751
SfaNI GCATC 3 cut(s) 451, 595, 666
SfcI CTRYAG 1 cut(s) 431
SfuI TTCGAA 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
SseBI AGGCCT 1 cut(s) 351
SspI AATATT 2 cut(s) 49, 510
SspMI CTAG 5 cut(s) 77, 291, 359, 488, 569
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 739
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 1 cut(s) 751
TaqI TCGA 1 cut(s) 23
TatI WGTACW 1 cut(s) 728
TfiI GAWTC 4 cut(s) 97, 218, 276, 317
Tru1I TTAA 4 cut(s) 165, 366, 629, 792
Tru9I TTAA 4 cut(s) 165, 366, 629, 792
TscAI CASTG 2 cut(s) 152, 708
TspRI CASTG 2 cut(s) 152, 708
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 2 cut(s) 369, 438
XmnI GAANNNNTTC 2 cut(s) 711, 750
XspI CTAG 5 cut(s) 77, 291, 359, 488, 569
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.