Rh1CG065500

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0018880
Physical Location & Seq
Forward (+)
13347689 .. 13354472
6784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG065500.1

Sequence Viewer

Length: 330 bp
ATGTCAGACTCCTCTTTTCATGCTTGTTGGAACACAAGCTATGCCATAGGCGGTATTGCGGGTGGCGAAAGTAAGGATTCATTTTGGCACATTGTTGCTCAGTGCACTGCTGCACTGCCTGAGGACAAGCTGCGATATGTTATGGGCGTTGGTTATCCATTGGACATTGTAGTTTGCAATGCTTTAGGTGCTGACATGTATGACTATGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTTTTATACCCAAGGGAACAGAAGCCATTAAAGGCATATTGTTATACTTGCCTGAATTAGAAGAGGTAGATTGTAATCTTGCAACTTGA

Protein Analysis

109

Amino Acids

11.88

Weight (kDa)

4.6

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 91 2.9e-28 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018880)

Species Orthologous Gene IDs
malus_domestica MD05G1028800.v1.1 MD10G1018600.v1.1
pyrus_communis pycom04g04790
rosa_laevigata RLG00000030175 RLG00000030179 RLG00000030182
rosa_samantha Rh1CG065500 Rh1DG070600
rosa_wichuraiana Rw1G005370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 51, 59
AfaI GTAC 1 cut(s) 223
AflIII ACRYGT 2 cut(s) 195, 218
AluBI AGCT 3 cut(s) 39, 130, 242
AluI AGCT 3 cut(s) 39, 130, 242
Alw21I GWGCWC 1 cut(s) 107
Alw44I GTGCAC 1 cut(s) 103
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 2 cut(s) 110, 130
AxyI CCTNAGG 1 cut(s) 120
BaeGI GKGCMC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 97, 117
BisI GCNGC 2 cut(s) 111, 131
BlsI GCNGC 2 cut(s) 112, 132
BsaAI YACGTR 1 cut(s) 221
BsaBI GATNNNNATC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 252
Bse21I CCTNAGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 252
BseJI GATNNNNATC 1 cut(s) 315
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 111, 113
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 2 cut(s) 97, 117
BsgI GTGCAG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 107
BspACI CCGC 2 cut(s) 51, 59
BspCNI CTCAG 2 cut(s) 112, 112
BsrDI GCAATG 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 297
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 99, 120
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 97, 117
Bsu36I CCTNAGG 1 cut(s) 120
BtsI GCAGTG 2 cut(s) 105, 113
BtsIMutI CAGTG 3 cut(s) 105, 107, 113
Cac8I GCNNGC 1 cut(s) 229
Csp6I GTAC 1 cut(s) 222
CviAII CATG 2 cut(s) 20, 196
CviJI RGCY 4 cut(s) 39, 130, 242, 266
CviKI_1 RGCY 4 cut(s) 39, 130, 242, 266
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 2 cut(s) 99, 120
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 252
Eco81I CCTNAGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 23, 199
FatI CATG 2 cut(s) 19, 195
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 111, 131
Fsp4HI GCNGC 2 cut(s) 111, 131
GluI GCNGC 2 cut(s) 111, 131
Hin1II CATG 2 cut(s) 23, 199
HinfI GANTC 2 cut(s) 8, 77
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 1 cut(s) 105
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 4 cut(s) 105, 113, 177, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 2 cut(s) 99, 120
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 2 cut(s) 23, 199
LpnPI CCDG 2 cut(s) 132, 306
Lsp1109I GCAGC 2 cut(s) 97, 117
MaeII ACGT 1 cut(s) 220
MboII GAAGA 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 1 cut(s) 296
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 2 cut(s) 8, 239
MnlI CCTC 3 cut(s) 22, 115, 298
MseI TTAA 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 23, 199
NspI RCATGY 1 cut(s) 199
PciI ACATGT 1 cut(s) 195
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 112, 132
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 221
PscI ACATGT 1 cut(s) 195
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 2 cut(s) 111, 131
SchI GAGTC 1 cut(s) 2
SduI GDGCHC 1 cut(s) 107
SetI ASST 6 cut(s) 41, 132, 190, 223, 244, 309
Sse9I AATT 1 cut(s) 296
SsiI CCGC 2 cut(s) 51, 59
StyI CCWWGG 1 cut(s) 252
TaiI ACGT 1 cut(s) 223
TasI AATT 1 cut(s) 296
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 3 cut(s) 107, 112, 120
TseI GCWGC 2 cut(s) 110, 130
TspDTI ATGAA 2 cut(s) 8, 69
TspRI CASTG 3 cut(s) 107, 112, 120
VneI GTGCAC 1 cut(s) 103
XceI RCATGY 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.