Rw1G005370

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018880
Physical Location & Seq
Forward (+)
10377337 .. 10378365
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005370.1

Sequence Viewer

Length: 276 bp
ATGTCAGACTCCTCTTTTCATGCTTGTTGGAACACAAGCTATGCCATAGGCGGTGTTGCGGGTGGTGAAAGTAAGGATTCATTTTGGCGCATTGTTGCTCAGTGCACTGCTGCACTACCTGAGGACAAGCCGCGATATGTTATGCGCGTTGGTTATCCATTGGACATTGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTGTGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTCTTATACCCGAGGTAATTACTTCTAAAATGTGA

Protein Analysis

91

Amino Acids

9.92

Weight (kDa)

5.63

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 85 3.1e-29 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018880)

Species Orthologous Gene IDs
malus_domestica MD05G1028800.v1.1 MD10G1018600.v1.1
pyrus_communis pycom04g04790
rosa_laevigata RLG00000030175 RLG00000030179 RLG00000030182
rosa_samantha Rh1CG065500 Rh1DG070600
rosa_wichuraiana Rw1G005370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 133, 147
AciI CCGC 3 cut(s) 51, 59, 131
AfaI GTAC 1 cut(s) 223
AflIII ACRYGT 2 cut(s) 195, 218
AluBI AGCT 2 cut(s) 39, 242
AluI AGCT 2 cut(s) 39, 242
Alw21I GWGCWC 1 cut(s) 107
Alw44I GTGCAC 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 251
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 110
AspLEI GCGC 2 cut(s) 90, 147
AsuHPI GGTGA 1 cut(s) 77
AvaI CYCGRG 1 cut(s) 251
AxyI CCTNAGG 1 cut(s) 120
BaeGI GKGCMC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 97
BisI GCNGC 2 cut(s) 111, 131
BlsI GCNGC 2 cut(s) 112, 132
BmeT110I CYCGRG 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 221
BsaJI CCNNGG 1 cut(s) 252
Bse21I CCTNAGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 111, 113
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 2 cut(s) 133, 147
BsiHKAI GWGCWC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 107
BspACI CCGC 3 cut(s) 51, 59, 131
BspCNI CTCAG 2 cut(s) 112, 112
BspFNI CGCG 2 cut(s) 133, 147
BssECI CCNNGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 206
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 99, 120
BstFNI CGCG 2 cut(s) 133, 147
BstHHI GCGC 2 cut(s) 90, 147
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 2 cut(s) 133, 147
BstV1I GCAGC 1 cut(s) 97
Bsu36I CCTNAGG 1 cut(s) 120
BtsI GCAGTG 2 cut(s) 105, 184
BtsIMutI CAGTG 3 cut(s) 105, 107, 184
Cac8I GCNNGC 1 cut(s) 229
CfoI GCGC 2 cut(s) 90, 147
Csp6I GTAC 1 cut(s) 222
CviAII CATG 2 cut(s) 20, 196
CviJI RGCY 3 cut(s) 39, 130, 242
CviKI_1 RGCY 3 cut(s) 39, 130, 242
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 2 cut(s) 99, 120
Eco81I CCTNAGG 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 251
FaeI CATG 2 cut(s) 23, 199
FaiI YATR 8 cut(s) 21, 42, 47, 138, 143, 197, 201, 248
FatI CATG 2 cut(s) 19, 195
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 111, 131
Fsp4HI GCNGC 2 cut(s) 111, 131
GlaI GCGC 2 cut(s) 89, 146
GluI GCNGC 2 cut(s) 111, 131
HhaI GCGC 2 cut(s) 90, 147
Hin1II CATG 2 cut(s) 23, 199
Hin6I GCGC 2 cut(s) 88, 145
HinP1I GCGC 2 cut(s) 88, 145
HinfI GANTC 2 cut(s) 8, 77
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 7
Hpy8I GTNNAC 1 cut(s) 105
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 3 cut(s) 105, 113, 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 2 cut(s) 99, 120
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 2 cut(s) 23, 199
HspAI GCGC 2 cut(s) 88, 145
LpnPI CCDG 1 cut(s) 132
Lsp1109I GCAGC 1 cut(s) 97
MaeII ACGT 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 1 cut(s) 258
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 2 cut(s) 8, 239
MnlI CCTC 3 cut(s) 22, 115, 247
MvnI CGCG 2 cut(s) 133, 147
MwoI GCNNNNNNNGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 23, 199
NspI RCATGY 1 cut(s) 199
PciI ACATGT 1 cut(s) 195
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 112, 132
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 221
PscI ACATGT 1 cut(s) 195
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SatI GCNGC 2 cut(s) 111, 131
SchI GAGTC 1 cut(s) 2
SduI GDGCHC 1 cut(s) 107
SetI ASST 6 cut(s) 41, 121, 190, 223, 244, 258
Sse9I AATT 1 cut(s) 258
SsiI CCGC 3 cut(s) 51, 59, 131
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 1 cut(s) 223
TasI AATT 1 cut(s) 258
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 1 cut(s) 77
TscAI CASTG 3 cut(s) 107, 112, 184
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 8, 69
TspRI CASTG 3 cut(s) 107, 112, 184
VneI GTGCAC 1 cut(s) 103
XceI RCATGY 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.