Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0018880

Physical Location & Seq

Genomic Coordinates Reverse (-)

56993132 .. 56997643

Gene Structure 4512 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030175

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGAAACTTATGGTGGTAGATAGGAAGAACTCAAGACGTCATCGGTGGCAAATCGAGGTTCTGAGGCAGTGGCGGCTTCGCAATTGTCGAAAGTGGCTGCAACTCGGGACTCGGCCTGCACCGATCAGATCTGTTGAGTTTTCAGAACTCCGCTCATGGCGTCGCTACTCGTGGAGTTGTGGCTCTGCTCTAAGCTACGACAACGCGTCTCGCTCCGAGCTTCTGAAGACTCAGGGAATCTCTCGTGTTCTCAACATTTCTAGTACTGGCTTCGATGCCTTAGCTTTATTCCCAGATTTCTATTGGAAGAACAGAGCAGCTAATGCAGCTAGTAATGGATGGGGAAGTCTACGTCGACGTGCTTCAAAGCGTCAATTCATGCTTGGTGAGCAGCTTCAGTTTCTTCCTAATCAAATCAAAGGGCAACCTCAAATGCTCAATAGGATTCATTCTGCCATAAACTCGGCTTCTCCTTTAGCATTTGAAGCTTCAAATTTGAGGTTTTGGGGAGGTTTAATAGCTGACACTCCCTCACTTTGTATGTCAGACCCCTCTTTTCATGCCTGTTGGAACACAAGCTATGCCACATATGGTCTTGCGGGTGGTAAAAGTAAGGATTCATTTTGGCGCATTGTTGCTCAGTGCACTGCTGCACTGCCTGAGGACAAGCGGCGATATGTTATGGGTGTTGGTTATCCATTGGACATTGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTCTGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTCTTATACCTGAGACAGATGGGCATCAGAAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008479 GO:0009451 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016740 GO:0016757 GO:0016763 GO:0019867 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046982 GO:0046983 GO:0071704 GO:0090304 GO:0098588 GO:0098805 GO:0101030 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TGT

Protein Analysis

273

Amino Acids

30.91

Weight (kDa)

10.1

Isoelectric Point (pI)

62.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGT	PF01702	193 - 265	7.5e-24	Queuine tRNA-ribosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0018880)

Species	Orthologous Gene IDs
malus_domestica	MD05G1028800.v1.1 MD10G1018600.v1.1
pyrus_communis	pycom04g04790
rosa_laevigata	RLG00000030175 RLG00000030179 RLG00000030182
rosa_samantha	Rh1CG065500 Rh1DG070600
rosa_wichuraiana	Rw1G005370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	40
AccBSI	CCGCTC	1 cut(s)	153
AccI	GTMKAC	2 cut(s)	349, 355
AccII	CGCG	1 cut(s)	206
AciI	CCGC	4 cut(s)	73, 151, 599, 670
AcsI	RAATTY	1 cut(s)	493
AcuI	CTGAAG	2 cut(s)	245, 380
AcyI	GRCGYC	2 cut(s)	37, 160
AfaI	GTAC	2 cut(s)	265, 763
AflIII	ACRYGT	3 cut(s)	204, 735, 758
AgsI	TTSAA	3 cut(s)	366, 485, 492
AhdI	GACNNNNNGTC	1 cut(s)	205
AjiI	CACGTC	1 cut(s)	359
Alw21I	GWGCWC	1 cut(s)	647
Alw26I	GTCTC	2 cut(s)	213, 788
Alw44I	GTGCAC	1 cut(s)	643
Ama87I	CYCGRG	1 cut(s)	104
AoxI	GGCC	1 cut(s)	113
ApaLI	GTGCAC	1 cut(s)	643
ApeKI	GCWGC	5 cut(s)	97, 317, 326, 391, 650
ApoI	RAATTY	1 cut(s)	493
AspLEI	GCGC	1 cut(s)	630
AsuHPI	GGTGA	1 cut(s)	398
AvaI	CYCGRG	1 cut(s)	104
AxyI	CCTNAGG	1 cut(s)	660
BaeGI	GKGCMC	1 cut(s)	647
BauI	CACGAG	2 cut(s)	169, 243
BbsI	GAAGAC	1 cut(s)	233
Bbv12I	GWGCWC	1 cut(s)	647
BbvI	GCAGC	5 cut(s)	84, 329, 338, 403, 637
BccI	CCATC	2 cut(s)	333, 794
BcoDI	GTCTC	2 cut(s)	213, 788
BfaI	CTAG	2 cut(s)	261, 330
BfmI	CTRYAG	1 cut(s)	815
BglII	AGATCT	1 cut(s)	128
BisI	GCNGC	7 cut(s)	74, 98, 318, 327, 392, 651, 671
BlsI	GCNGC	7 cut(s)	75, 99, 319, 328, 393, 652, 672
BmcAI	AGTACT	1 cut(s)	265
BmeRI	GACNNNNNGTC	1 cut(s)	205
BmeT110I	CYCGRG	1 cut(s)	104
BmgBI	CACGTC	1 cut(s)	359
BmsI	GCATC	2 cut(s)	265, 814
BpiI	GAAGAC	1 cut(s)	233
Bpu10I	CCTNAGC	1 cut(s)	280
BpuEI	CTTGAG	1 cut(s)	16
BsaAI	YACGTR	1 cut(s)	761
BsaBI	GATNNNNATC	1 cut(s)	804
BsaHI	GRCGYC	2 cut(s)	37, 160
Bse1I	ACTGG	1 cut(s)	271
Bse21I	CCTNAGG	1 cut(s)	660
Bse8I	GATNNNNATC	1 cut(s)	804
BseGI	GGATG	1 cut(s)	344
BseJI	GATNNNNATC	1 cut(s)	804
BseMII	CTCAG	5 cut(s)	53, 245, 651, 653, 783
BseNI	ACTGG	1 cut(s)	271
BseSI	GKGCMC	1 cut(s)	647
BseXI	GCAGC	5 cut(s)	84, 329, 338, 403, 637
BsgI	GTGCAG	2 cut(s)	102, 636
Bsh1236I	CGCG	1 cut(s)	206
BshFI	GGCC	1 cut(s)	115
BsiHKAI	GWGCWC	1 cut(s)	647
BsiHKCI	CYCGRG	1 cut(s)	104
BslFI	GGGAC	1 cut(s)	121
BsmAI	GTCTC	2 cut(s)	213, 788
BsmBI	CGTCTC	1 cut(s)	213
BsmFI	GGGAC	1 cut(s)	121
BsnI	GGCC	1 cut(s)	115
BsoBI	CYCGRG	1 cut(s)	104
Bsp1286I	GDGCHC	1 cut(s)	647
Bsp143I	GATC	2 cut(s)	123, 128
BspACI	CCGC	4 cut(s)	73, 151, 599, 670
BspANI	GGCC	1 cut(s)	115
BspCNI	CTCAG	5 cut(s)	54, 244, 652, 652, 784
BspFNI	CGCG	1 cut(s)	206
BsrBI	CCGCTC	1 cut(s)	153
BsrI	ACTGG	1 cut(s)	271
BssMI	GATC	2 cut(s)	123, 128
BssNI	GRCGYC	2 cut(s)	37, 160
BssSI	CACGAG	2 cut(s)	169, 243
Bst2BI	CACGAG	2 cut(s)	169, 243
BstACI	GRCGYC	2 cut(s)	37, 160
BstAPI	GCANNNNNTGC	1 cut(s)	323
BstBAI	YACGTR	1 cut(s)	761
BstC8I	GCNNGC	2 cut(s)	117, 769
BstDEI	CTNAG	7 cut(s)	62, 191, 231, 280, 639, 660, 792
BstF5I	GGATG	1 cut(s)	344
BstFNI	CGCG	1 cut(s)	206
BstHHI	GCGC	1 cut(s)	630
BstKTI	GATC	2 cut(s)	126, 131
BstMAI	GTCTC	2 cut(s)	213, 788
BstMBI	GATC	2 cut(s)	123, 128
BstMWI	GCNNNNNNNGC	6 cut(s)	73, 323, 326, 388, 485, 728
BstNSI	RCATGY	1 cut(s)	739
BstSFI	CTRYAG	1 cut(s)	815
BstSLI	GKGCMC	1 cut(s)	647
BstUI	CGCG	1 cut(s)	206
BstV1I	GCAGC	5 cut(s)	84, 329, 338, 403, 637
BstV2I	GAAGAC	1 cut(s)	233
BstX2I	RGATCY	1 cut(s)	128
BstYI	RGATCY	1 cut(s)	128
Bsu36I	CCTNAGG	1 cut(s)	660
BsuRI	GGCC	1 cut(s)	115
BtrI	CACGTC	1 cut(s)	359
BtsCI	GGATG	1 cut(s)	344
BtsI	GCAGTG	4 cut(s)	74, 645, 653, 724
BtsIMutI	CAGTG	5 cut(s)	74, 645, 647, 653, 724
Cac8I	GCNNGC	2 cut(s)	117, 769
CfoI	GCGC	1 cut(s)	630
CseI	GACGC	3 cut(s)	149, 195, 359
Csp6I	GTAC	2 cut(s)	264, 762
CviAII	CATG	4 cut(s)	156, 379, 560, 736
CviQI	GTAC	2 cut(s)	264, 762
DdeI	CTNAG	7 cut(s)	62, 191, 231, 280, 639, 660, 792
DpnI	GATC	2 cut(s)	125, 130
DpnII	GATC	2 cut(s)	123, 128
DriI	GACNNNNNGTC	1 cut(s)	205
Eam1105I	GACNNNNNGTC	1 cut(s)	205
Eco57I	CTGAAG	2 cut(s)	245, 380
Eco81I	CCTNAGG	1 cut(s)	660
Eco88I	CYCGRG	1 cut(s)	104
Esp3I	CGTCTC	1 cut(s)	213
FaeI	CATG	4 cut(s)	159, 382, 563, 739
FaqI	GGGAC	1 cut(s)	121
FatI	CATG	4 cut(s)	155, 378, 559, 735
FauI	CCCGC	1 cut(s)	592
FauNDI	CATATG	1 cut(s)	589
FblI	GTMKAC	2 cut(s)	349, 355
Fnu4HI	GCNGC	7 cut(s)	74, 98, 318, 327, 392, 651, 671
FokI	GGATG	1 cut(s)	351
Fsp4HI	GCNGC	7 cut(s)	74, 98, 318, 327, 392, 651, 671
FspBI	CTAG	2 cut(s)	261, 330
GlaI	GCGC	1 cut(s)	629
GluI	GCNGC	7 cut(s)	74, 98, 318, 327, 392, 651, 671
HaeIII	GGCC	1 cut(s)	115
HgaI	GACGC	3 cut(s)	149, 195, 359
HhaI	GCGC	1 cut(s)	630
Hin1I	GRCGYC	2 cut(s)	37, 160
Hin1II	CATG	4 cut(s)	159, 382, 563, 739
Hin6I	GCGC	1 cut(s)	628
HinP1I	GCGC	1 cut(s)	628
HincII	GTYRAC	1 cut(s)	356
HindII	GTYRAC	1 cut(s)	356
HindIII	AAGCTT	1 cut(s)	486
HinfI	GANTC	6 cut(s)	109, 229, 237, 445, 617, 743
HphI	GGTGA	1 cut(s)	398
Hpy166II	GTNNAC	3 cut(s)	350, 356, 645
Hpy188I	TCNGA	7 cut(s)	63, 128, 145, 217, 225, 547, 810
Hpy188III	TCNNGA	2 cut(s)	33, 106
Hpy8I	GTNNAC	3 cut(s)	350, 356, 645
Hpy99I	CGWCG	3 cut(s)	165, 357, 360
HpyCH4IV	ACGT	4 cut(s)	37, 352, 358, 760
HpyCH4V	TGCA	6 cut(s)	100, 119, 326, 645, 653, 717
HpyF10VI	GCNNNNNNNGC	6 cut(s)	73, 323, 326, 388, 485, 728
HpyF3I	CTNAG	7 cut(s)	62, 191, 231, 280, 639, 660, 792
HpySE526I	ACGT	4 cut(s)	37, 352, 358, 760
Hsp92I	GRCGYC	2 cut(s)	37, 160
Hsp92II	CATG	4 cut(s)	159, 382, 563, 739
HspAI	GCGC	1 cut(s)	628
Kzo9I	GATC	2 cut(s)	123, 128
LmnI	GCTCC	1 cut(s)	218
LpnPI	CCDG	7 cut(s)	129, 218, 252, 306, 577, 672, 804
Lsp1109I	GCAGC	5 cut(s)	84, 329, 338, 403, 637
LweI	GCATC	2 cut(s)	265, 814
MaeI	CTAG	2 cut(s)	261, 330
MaeII	ACGT	4 cut(s)	37, 352, 358, 760
MalI	GATC	2 cut(s)	125, 130
MbiI	CCGCTC	1 cut(s)	153
MboI	GATC	2 cut(s)	123, 128
MboII	GAAGA	4 cut(s)	37, 238, 319, 395
MfeI	CAATTG	1 cut(s)	82
MflI	RGATCY	1 cut(s)	128
MhlI	GDGCHC	1 cut(s)	647
MluCI	AATT	3 cut(s)	82, 374, 493
MluI	ACGCGT	1 cut(s)	204
MlyI	GAGTC	3 cut(s)	103, 223, 737
MmeI	TCCRAC	2 cut(s)	548, 779
MnlI	CCTC	8 cut(s)	49, 57, 438, 492, 503, 541, 562, 655
MseI	TTAA	1 cut(s)	515
MunI	CAATTG	1 cut(s)	82
MvnI	CGCG	1 cut(s)	206
MwoI	GCNNNNNNNGC	6 cut(s)	73, 323, 326, 388, 485, 728
NdeI	CATATG	1 cut(s)	589
NdeII	GATC	2 cut(s)	123, 128
NlaIII	CATG	4 cut(s)	159, 382, 563, 739
NmeAIII	GCCGAG	2 cut(s)	91, 443
NspI	RCATGY	1 cut(s)	739
PciI	ACATGT	1 cut(s)	735
PcsI	WCGNNNNNNNCGW	1 cut(s)	85
PfeI	GAWTC	3 cut(s)	237, 445, 617
PkrI	GCNGC	7 cut(s)	75, 99, 319, 328, 393, 652, 672
PleI	GAGTC	3 cut(s)	103, 223, 737
PpsI	GAGTC	3 cut(s)	103, 223, 737
Ppu21I	YACGTR	1 cut(s)	761
PscI	ACATGT	1 cut(s)	735
PsuI	RGATCY	1 cut(s)	128
RsaI	GTAC	2 cut(s)	265, 763
RsaNI	GTAC	2 cut(s)	264, 762
SalI	GTCGAC	1 cut(s)	354
SaqAI	TTAA	1 cut(s)	515
SatI	GCNGC	7 cut(s)	74, 98, 318, 327, 392, 651, 671
Sau3AI	GATC	2 cut(s)	123, 128
ScaI	AGTACT	1 cut(s)	265
SchI	GAGTC	3 cut(s)	103, 223, 737
SduI	GDGCHC	1 cut(s)	647
SfaNI	GCATC	2 cut(s)	265, 814
SfcI	CTRYAG	1 cut(s)	815
SgrDI	CGTCGACG	1 cut(s)	354
SmlI	CTYRAG	1 cut(s)	31
SmoI	CTYRAG	1 cut(s)	31
Sse9I	AATT	3 cut(s)	82, 374, 493
SsiI	CCGC	4 cut(s)	73, 151, 599, 670
SspMI	CTAG	2 cut(s)	261, 330
TaiI	ACGT	4 cut(s)	40, 355, 361, 763
TaqI	TCGA	4 cut(s)	54, 88, 273, 355
TasI	AATT	3 cut(s)	82, 374, 493
TatI	WGTACW	1 cut(s)	263
TauI	GCSGC	2 cut(s)	76, 673
TfiI	GAWTC	3 cut(s)	237, 445, 617
Tru1I	TTAA	1 cut(s)	515
Tru9I	TTAA	1 cut(s)	515
TscAI	CASTG	5 cut(s)	74, 647, 652, 660, 724
TseI	GCWGC	5 cut(s)	97, 317, 326, 391, 650
TspDTI	ATGAA	5 cut(s)	17, 367, 437, 548, 609
TspRI	CASTG	5 cut(s)	74, 647, 652, 660, 724
VneI	GTGCAC	1 cut(s)	643
XapI	RAATTY	1 cut(s)	493
XceI	RCATGY	1 cut(s)	739
XcmI	CCANNNNNNNNNTGG	1 cut(s)	300
XmiI	GTMKAC	2 cut(s)	349, 355
XspI	CTAG	2 cut(s)	261, 330
ZraI	GACGTC	1 cut(s)	38
ZrmI	AGTACT	1 cut(s)	265

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.