RLG00000030179

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018880
Physical Location & Seq
Reverse (-)
57092095 .. 57094431
2337 bp
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UTR
Exon/CDS
Intron
RLM00000030179

Sequence Viewer

Length: 441 bp
ATGCTTGGTGAGCAGCTTCAGTTTCTTCCTAATCAAATCAAAGGGCAACCTCAAATGCTCAATAGGATTCATTCTGCCATAAACTCGGCTTCTCCTTTAGCATTTGAAGCTTCAAATTTGAGGTTTTGGGGAGGTTTAATAGCTGACACTCCCTCACTTTGTATGTCAGACCCCTCTTTTCATGCCTGTTGGAACACAAGCTATGCCACATATGGTCTTGCGGGTGGTGAAAGTAAGGATTCATTTTGGCGCATTGTTGCTCAGTGCACTGCTGCACTGCGTGAGGACAAGCGGAGATATGTTATGGGCGTTGGTTATCCATTGGACATAGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTGTGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTCTTATTCATGAGACAGATGGGCATCAGAAACTATAG

Protein Analysis

147

Amino Acids

16.12

Weight (kDa)

6.39

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 67 - 139 3.3e-25 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018880)

Species Orthologous Gene IDs
malus_domestica MD05G1028800.v1.1 MD10G1018600.v1.1
pyrus_communis pycom04g04790
rosa_laevigata RLG00000030175 RLG00000030179 RLG00000030182
rosa_samantha Rh1CG065500 Rh1DG070600
rosa_wichuraiana Rw1G005370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 221, 292
AcsI RAATTY 1 cut(s) 115
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 1 cut(s) 385
AflIII ACRYGT 2 cut(s) 357, 380
AgsI TTSAA 2 cut(s) 107, 114
AluBI AGCT 5 cut(s) 16, 110, 143, 201, 404
AluI AGCT 5 cut(s) 16, 110, 143, 201, 404
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 1 cut(s) 410
Alw44I GTGCAC 1 cut(s) 265
ApaLI GTGCAC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 13, 272
ApoI RAATTY 1 cut(s) 115
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 20, 239
BaeGI GKGCMC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 2 cut(s) 25, 259
BccI CCATC 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 437
BisI GCNGC 2 cut(s) 14, 273
BlsI GCNGC 2 cut(s) 15, 274
BmsI GCATC 1 cut(s) 436
BsaAI YACGTR 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 426
Bse8I GATNNNNATC 1 cut(s) 426
BseJI GATNNNNATC 1 cut(s) 426
BseMII CTCAG 1 cut(s) 275
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 2 cut(s) 25, 259
BsgI GTGCAG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 269
BspACI CCGC 2 cut(s) 221, 292
BspCNI CTCAG 1 cut(s) 274
BspHI TCATGA 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 368
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 410
BstMWI GCNNNNNNNGC 3 cut(s) 10, 107, 350
BstNSI RCATGY 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 437
BstSLI GKGCMC 1 cut(s) 269
BstV1I GCAGC 2 cut(s) 25, 259
BtsI GCAGTG 3 cut(s) 267, 275, 346
BtsIMutI CAGTG 4 cut(s) 267, 269, 275, 346
Cac8I GCNNGC 1 cut(s) 391
CciI TCATGA 1 cut(s) 412
CfoI GCGC 1 cut(s) 252
Csp6I GTAC 1 cut(s) 384
CviAII CATG 3 cut(s) 182, 358, 413
CviJI RGCY 6 cut(s) 16, 89, 110, 143, 201, 404
CviKI_1 RGCY 6 cut(s) 16, 89, 110, 143, 201, 404
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 1 cut(s) 261
DraIII CACNNNGTG 1 cut(s) 281
FaeI CATG 3 cut(s) 185, 361, 416
FatI CATG 3 cut(s) 181, 357, 412
FauI CCCGC 1 cut(s) 214
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 14, 273
Fsp4HI GCNGC 2 cut(s) 14, 273
GlaI GCGC 1 cut(s) 251
GluI GCNGC 2 cut(s) 14, 273
HhaI GCGC 1 cut(s) 252
Hin1II CATG 3 cut(s) 185, 361, 416
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 2 cut(s) 67, 239
HphI GGTGA 2 cut(s) 20, 239
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 2 cut(s) 169, 432
Hpy188III TCNNGA 1 cut(s) 413
Hpy8I GTNNAC 1 cut(s) 267
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 3 cut(s) 267, 275, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 107, 350
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 3 cut(s) 185, 361, 416
HspAI GCGC 1 cut(s) 250
LpnPI CCDG 1 cut(s) 199
Lsp1109I GCAGC 2 cut(s) 25, 259
LweI GCATC 1 cut(s) 436
MaeII ACGT 1 cut(s) 382
MboII GAAGA 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 1 cut(s) 115
MmeI TCCRAC 2 cut(s) 170, 401
MnlI CCTC 6 cut(s) 60, 114, 125, 163, 184, 277
MseI TTAA 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 10, 107, 350
NdeI CATATG 1 cut(s) 211
NlaIII CATG 3 cut(s) 185, 361, 416
NmeAIII GCCGAG 1 cut(s) 65
NspI RCATGY 1 cut(s) 361
PagI TCATGA 1 cut(s) 412
PciI ACATGT 1 cut(s) 357
PfeI GAWTC 2 cut(s) 67, 239
PkrI GCNGC 2 cut(s) 15, 274
Ppu21I YACGTR 1 cut(s) 383
PscI ACATGT 1 cut(s) 357
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 2 cut(s) 14, 273
SduI GDGCHC 1 cut(s) 269
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 437
Sse9I AATT 1 cut(s) 115
SsiI CCGC 2 cut(s) 221, 292
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 1 cut(s) 385
TasI AATT 1 cut(s) 115
TfiI GAWTC 2 cut(s) 67, 239
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TscAI CASTG 4 cut(s) 269, 274, 282, 346
TseI GCWGC 2 cut(s) 13, 272
TspDTI ATGAA 4 cut(s) 59, 170, 231, 401
TspRI CASTG 4 cut(s) 269, 274, 282, 346
VneI GTGCAC 1 cut(s) 265
XapI RAATTY 1 cut(s) 115
XceI RCATGY 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.