MD05G1034700.v1.1

Large subunit GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010426
Physical Location & Seq
Forward (+)
5618955 .. 5622175
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1034700.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGGGTAAGAACGAGAAGACGGGGCTGGGTAGGGCCCTTGTGAGACAGCATAACCAACTGGTTCAGCAGACGAAAGAGAAAGGCCTGATGTACAAGAGGCAGCAGAAGAAGGTTCTCGAATCCGTCACCGAAGTCAGCGACATCGACGCCATCGTCGAAAAAGCCGACGAAGCCGACCGCCTCTTCTCCCTCGACCACGCTGCCCCTAACCTCCTCATCGACCTAGATGGGAATGAGATAACCCCGGAGCAGCGGAGGGAGCAGAAGAGGAAAGAGGAGGCCCTGCACGCCGGAAGTCTGCGCGTGCCGCGGAGGCCTCCGTGGACCCCTCAAATGTCTGTGGAGGAGCTGGATAACAATGAAAGACAGGCCTTCTTGACCTGGCGCAGAAGCCTTGCCAGGCTTGAGGAGAACGACAAGCTTCTCCTTACTCCTTTCGAGAAGAACCTCGATATTTGGAGGCAGCTGTGGCGGGTTGTCGAGCGTAGCGATTTGATTGTGATGGTTGTTGATGCGCGAGACCCTCTGTTCTATCGCTGCCCCGATCTTGAGGTTTATGCACGAGAGGTCGACAAGCACAAAAGGACAATGCTTCTTGTTAACAAAGCAGATCTCTTACCTTTATCTGTTAGGGAGAAGTGGGCACAATATTTCCGTTCGCAAGATATTCTCTTTGTGTTTTGGTCGGCTAAAGCTGCTACAGCAGCTACAGAAGGGAAAAGTCTCAGTTCCCCGTGGACAGAAAATAGCCTGCAGGAGTCAGAAGACCCTGATACAAAAATATATGGGAGGATTGAGCTTTTGGCTCGTTTACAATCTGAGGCAGAAGAGATAGTAAAACTGAGGAAATCAGGATCCAGTGGTTCTCGAAGTGGAAATATTGTTAGAAATTCAGCTTCTAGTAATGTAGTTGTGGGATTTGTTGGATATCCAAATGTGGGAAAGAGCTCGACAATTAATGCCTTGGTAGGCCAGAAGAAGACAGGTGTTACCTCTACTCCTGGGAAGACAAAGCATTTCCAAACATTGATTATGTCTGACGAGTTAATGCTATGTGATTGCCCTGGATTGGTCTTTCCATCCTTTTCAAGCTCAAGACATGAAATGATTGCTTCTGGTGTATTGCCAATTGATCGAATGACTGAGAATAGGGAGGCTGTGCAGGTAGTGGCCAATCGAATTCCACGACATGTTATTGAAGCGGTTTATAGGATTGATCTGCCCAAGCCCAAATCCTATGAACTGCAGTCTCGACCACCTTTGGCAGCAGAATTTCTGAGGGTCTATTGTGCCTCTCGTGGTTATGTCGCCTCAAGTGGGCTTCCTGATGAAACGAGAGCTGCCCGCCAAATTTTGAAGGATTACATTGATGGGAAGCTGCCCCATTTTCAAATGCCCCCTGGAATGACTACTGAAGAAGATGCTGTGGGAAACGGCTTATCTGAGCAGCATAAATCAGACTCCTCTGAGCATGAACACTCTGTAGATGATGAAGGTGAGGACGAGCCAGAGCTTGATCATGTCTTGGAAGACCTCAATTCATTTGACTTAGCTAATGGGCTTGCTACCAAGAAGAAAGTCACTGTCAGAAAGCCAACTGCACCCCATAAACAACACAAAAAGACTCAGAGGAAGAAGGATCGCTCCTGGAGGGTAGGGAATGACAGTGGCGATGGAATGCCAGTTGTAAGAGTCTTTCAAAAACCAGCAAACACCGGTCCTCTTAAGGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005783 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010154 GO:0010467 GO:0010646 GO:0010817 GO:0012505 GO:0015030 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016072 GO:0016462 GO:0016604 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0022414 GO:0022613 GO:0023051 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042254 GO:0042886 GO:0043021 GO:0043022 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046626 GO:0046907 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051049 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0090069 GO:0090070 GO:0090304 GO:0097159 GO:0097367 GO:0099402 GO:1900076 GO:1901265 GO:1901360 GO:1901363 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

579

Amino Acids

65.24

Weight (kDa)

8.13

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 305 - 375 2.2e-15 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 1153
AccI GTMKAC 1 cut(s) 570
AccII CGCG 3 cut(s) 303, 310, 517
AciI CCGC 7 cut(s) 178, 253, 308, 310, 472, 1202, 1345
AclWI GGATC 3 cut(s) 849, 862, 1647
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 4 cut(s) 889, 1179, 1271, 1350
AcuI CTGAAG 1 cut(s) 1434
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 3 cut(s) 938, 1069, 1317
AflII CTTAAG 1 cut(s) 1724
AflIII ACRYGT 1 cut(s) 1189
AgeI ACCGGT 1 cut(s) 1715
AgsI TTSAA 5 cut(s) 1089, 1199, 1357, 1391, 1700
AjnI CCWGG 6 cut(s) 380, 398, 1000, 1063, 1399, 1646
Alw21I GWGCWC 1 cut(s) 950
Alw26I GTCTC 4 cut(s) 37, 513, 728, 1254
AlwI GGATC 3 cut(s) 849, 862, 1647
AoxI GGCC 7 cut(s) 33, 82, 279, 314, 369, 970, 1170
ApaI GGGCCC 1 cut(s) 37
ApoI RAATTY 4 cut(s) 889, 1179, 1271, 1350
AseI ATTAAT 1 cut(s) 957
AsiGI ACCGGT 1 cut(s) 1715
AspLEI GCGC 3 cut(s) 303, 387, 517
AspS9I GGNCC 5 cut(s) 33, 34, 280, 324, 1718
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 118, 1508
AvaII GGWCC 2 cut(s) 324, 1718
BaeGI GKGCMC 2 cut(s) 37, 646
BalI TGGCCA 1 cut(s) 1172
BamHI GGATCC 1 cut(s) 854
BanII GRGCYC 2 cut(s) 37, 950
BauI CACGAG 2 cut(s) 561, 1296
BbsI GAAGAC 5 cut(s) 23, 771, 986, 1013, 1536
Bbv12I GWGCWC 1 cut(s) 950
BccI CCATC 6 cut(s) 158, 221, 496, 1087, 1364, 1667
BceAI ACGGC 1 cut(s) 1450
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 6 cut(s) 382, 400, 1002, 1065, 1401, 1648
BclI TGATCA 1 cut(s) 1516
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 4 cut(s) 37, 513, 728, 1254
BfaI CTAG 2 cut(s) 224, 900
BfmI CTRYAG 5 cut(s) 699, 708, 752, 1244, 1482
BfrI CTTAAG 1 cut(s) 1724
BfuAI ACCTGC 1 cut(s) 1153
BglI GCCNNNNNGGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 610
Bme1390I CCNGG 7 cut(s) 245, 382, 400, 1002, 1065, 1401, 1648
Bme18I GGWCC 2 cut(s) 324, 1718
BmgT120I GGNCC 5 cut(s) 33, 34, 280, 324, 1718
BmiI GGNNCC 3 cut(s) 35, 326, 856
BmrFI CCNGG 7 cut(s) 245, 382, 400, 1002, 1065, 1401, 1648
BmsI GCATC 2 cut(s) 502, 1411
BpiI GAAGAC 5 cut(s) 23, 771, 986, 1013, 1536
BpmI CTGGAG 1 cut(s) 1669
BpuEI CTTGAG 4 cut(s) 425, 569, 1078, 1297
BpuMI CCSGG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 513
BsaJI CCNNGG 8 cut(s) 243, 308, 320, 734, 963, 1001, 1063, 1399
BsaWI WCCGGW 1 cut(s) 1715
BsaXI ACNNNNNCTCC 2 cut(s) 982, 1012
Bsc4I CCNNNNNNNGG 3 cut(s) 938, 1069, 1317
Bse118I RCCGGY 1 cut(s) 1715
Bse1I ACTGG 3 cut(s) 63, 858, 1682
BseBI CCWGG 6 cut(s) 382, 400, 1002, 1065, 1401, 1648
BseDI CCNNGG 8 cut(s) 243, 308, 320, 734, 963, 1001, 1063, 1399
BseGI GGATG 1 cut(s) 1079
BseLI CCNNNNNNNGG 3 cut(s) 938, 1069, 1317
BseMII CTCAG 8 cut(s) 739, 810, 833, 1134, 1268, 1434, 1458, 1640
BseNI ACTGG 3 cut(s) 63, 858, 1682
BseRI GAGGAG 5 cut(s) 203, 290, 359, 422, 1453
BseSI GKGCMC 2 cut(s) 37, 646
BseYI CCCAGC 1 cut(s) 25
BsgI GTGCAG 3 cut(s) 269, 1181, 1584
Bsh1236I CGCG 3 cut(s) 303, 310, 517
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 7 cut(s) 35, 84, 281, 316, 371, 972, 1172
BshTI ACCGGT 1 cut(s) 1715
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 950
BsiSI CCGG 3 cut(s) 245, 291, 1716
BslI CCNNNNNNNGG 3 cut(s) 938, 1069, 1317
BsmAI GTCTC 4 cut(s) 37, 513, 728, 1254
BsmI GAATGC 1 cut(s) 1683
BsnI GGCC 7 cut(s) 35, 84, 281, 316, 371, 972, 1172
Bso31I GGTCTC 1 cut(s) 513
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 37, 646, 950
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
BspACI CCGC 7 cut(s) 178, 253, 308, 310, 472, 1202, 1345
BspANI GGCC 7 cut(s) 35, 84, 281, 316, 371, 972, 1172
BspCNI CTCAG 8 cut(s) 738, 811, 834, 1135, 1269, 1435, 1459, 1639
BspFNI CGCG 3 cut(s) 303, 310, 517
BspLI GGNNCC 3 cut(s) 35, 326, 856
BspMAI CTGCAG 2 cut(s) 756, 1248
BspMI ACCTGC 1 cut(s) 1153
BspPI GGATC 3 cut(s) 849, 862, 1647
BspTI CTTAAG 1 cut(s) 1724
BspTNI GGTCTC 1 cut(s) 513
BsrFI RCCGGY 1 cut(s) 1715
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 3 cut(s) 63, 858, 1682
BssAI RCCGGY 1 cut(s) 1715
BssECI CCNNGG 8 cut(s) 243, 308, 320, 734, 963, 1001, 1063, 1399
BssMI GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 2 cut(s) 561, 1296
BssT1I CCWWGG 1 cut(s) 963
Bst2BI CACGAG 2 cut(s) 561, 1296
Bst2UI CCWGG 6 cut(s) 382, 400, 1002, 1065, 1401, 1648
Bst4CI ACNGT 2 cut(s) 1585, 1667
Bst6I CTCTTC 3 cut(s) 188, 260, 822
BstACI GRCGYC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 1724
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 5 cut(s) 288, 305, 752, 1345, 1563
BstDEI CTNAG 9 cut(s) 725, 819, 842, 1143, 1277, 1443, 1467, 1549, 1626
BstDSI CCRYGG 3 cut(s) 308, 320, 734
BstF5I GGATG 1 cut(s) 1079
BstFNI CGCG 3 cut(s) 303, 310, 517
BstHHI GCGC 3 cut(s) 303, 387, 517
BstKTI GATC 7 cut(s) 547, 613, 857, 1135, 1219, 1519, 1642
BstMAI GTCTC 4 cut(s) 37, 513, 728, 1254
BstMBI GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 9 cut(s) 170, 259, 287, 307, 313, 469, 695, 701, 704
BstNI CCWGG 6 cut(s) 382, 400, 1002, 1065, 1401, 1648
BstNSI RCATGY 1 cut(s) 1193
BstSCI CCNGG 7 cut(s) 243, 380, 398, 1000, 1063, 1399, 1646
BstSFI CTRYAG 5 cut(s) 699, 708, 752, 1244, 1482
BstSLI GKGCMC 2 cut(s) 37, 646
BstUI CGCG 3 cut(s) 303, 310, 517
BstV2I GAAGAC 5 cut(s) 23, 771, 986, 1013, 1536
BstX2I RGATCY 2 cut(s) 610, 854
BstYI RGATCY 2 cut(s) 610, 854
BsuRI GGCC 7 cut(s) 35, 84, 281, 316, 371, 972, 1172
BtgI CCRYGG 3 cut(s) 308, 320, 734
BtgZI GCGATG 1 cut(s) 1686
BtsCI GGATG 1 cut(s) 1079
BtsIMutI CAGTG 3 cut(s) 865, 1581, 1672
BveI ACCTGC 1 cut(s) 1153
Cac8I GCNNGC 5 cut(s) 288, 305, 752, 1345, 1563
CfoI GCGC 3 cut(s) 303, 387, 517
Cfr10I RCCGGY 1 cut(s) 1715
Cfr13I GGNCC 5 cut(s) 33, 34, 280, 324, 1718
Cfr42I CCGCGG 1 cut(s) 311
CseI GACGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 91
CspAI ACCGGT 1 cut(s) 1715
CviAII CATG 4 cut(s) 1100, 1190, 1472, 1520
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 9 cut(s) 725, 819, 842, 1143, 1277, 1443, 1467, 1549, 1626
DpnI GATC 7 cut(s) 546, 612, 856, 1134, 1218, 1518, 1641
DpnII GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 3 cut(s) 188, 260, 822
EarI CTCTTC 3 cut(s) 188, 260, 822
Ecl136II GAGCTC 1 cut(s) 948
Eco130I CCWWGG 1 cut(s) 963
Eco147I AGGCCT 3 cut(s) 84, 316, 371
Eco24I GRGCYC 2 cut(s) 37, 950
Eco31I GGTCTC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 929
Eco47I GGWCC 2 cut(s) 324, 1718
Eco53kI GAGCTC 1 cut(s) 948
Eco57I CTGAAG 1 cut(s) 1434
EcoICRI GAGCTC 1 cut(s) 948
EcoO109I RGGNCCY 3 cut(s) 33, 34, 280
EcoRI GAATTC 1 cut(s) 1179
EcoRII CCWGG 6 cut(s) 380, 398, 1000, 1063, 1399, 1646
EcoRV GATATC 1 cut(s) 929
EcoT14I CCWWGG 1 cut(s) 963
EcoT38I GRGCYC 2 cut(s) 37, 950
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 4 cut(s) 1103, 1193, 1475, 1523
FatI CATG 4 cut(s) 1099, 1189, 1471, 1519
FauI CCCGC 2 cut(s) 465, 1352
FbaI TGATCA 1 cut(s) 1516
FblI GTMKAC 1 cut(s) 570
FokI GGATG 1 cut(s) 1066
FriOI GRGCYC 2 cut(s) 37, 950
FspBI CTAG 2 cut(s) 224, 900
GlaI GCGC 3 cut(s) 302, 386, 516
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 1669
HaeIII GGCC 7 cut(s) 35, 84, 281, 316, 371, 972, 1172
HapII CCGG 3 cut(s) 245, 291, 1716
HgaI GACGC 1 cut(s) 155
HhaI GCGC 3 cut(s) 303, 387, 517
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 1103, 1193, 1475, 1523
Hin6I GCGC 3 cut(s) 301, 385, 515
HinP1I GCGC 3 cut(s) 301, 385, 515
HincII GTYRAC 2 cut(s) 571, 601
HindII GTYRAC 2 cut(s) 571, 601
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 5 cut(s) 119, 758, 1460, 1624, 1692
HpaI GTTAAC 1 cut(s) 601
HpaII CCGG 3 cut(s) 245, 291, 1716
HphI GGTGA 2 cut(s) 118, 1508
Hpy166II GTNNAC 5 cut(s) 324, 571, 601, 738, 812
Hpy188I TCNGA 9 cut(s) 763, 820, 1039, 1278, 1444, 1459, 1468, 1589, 1629
Hpy188III TCNNGA 9 cut(s) 116, 376, 439, 548, 852, 867, 1095, 1251, 1325
Hpy8I GTNNAC 5 cut(s) 324, 571, 601, 738, 812
Hpy99I CGWCG 3 cut(s) 149, 158, 170
HpyAV CCTTC 6 cut(s) 103, 382, 707, 1351, 1487, 1630
HpyCH4III ACNGT 2 cut(s) 1585, 1667
HpyCH4V TGCA 6 cut(s) 286, 560, 754, 1162, 1246, 1601
HpyF10VI GCNNNNNNNGC 9 cut(s) 170, 259, 287, 307, 313, 469, 695, 701, 704
HpyF3I CTNAG 9 cut(s) 725, 819, 842, 1143, 1277, 1443, 1467, 1549, 1626
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 1103, 1193, 1475, 1523
HspAI GCGC 3 cut(s) 301, 385, 515
Ksp22I TGATCA 1 cut(s) 1516
KspAI GTTAAC 1 cut(s) 601
KspI CCGCGG 1 cut(s) 311
Kzo9I GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
LmnI GCTCC 4 cut(s) 247, 259, 346, 1649
LweI GCATC 2 cut(s) 502, 1411
MaeI CTAG 2 cut(s) 224, 900
MaeIII GTNAC 3 cut(s) 124, 988, 1579
MalI GATC 7 cut(s) 546, 612, 856, 1134, 1218, 1518, 1641
MboI GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
MfeI CAATTG 1 cut(s) 1128
MflI RGATCY 2 cut(s) 610, 854
MhlI GDGCHC 3 cut(s) 37, 646, 950
MlsI TGGCCA 1 cut(s) 1172
MluCI AATT 7 cut(s) 889, 954, 1128, 1179, 1271, 1350, 1537
MluNI TGGCCA 1 cut(s) 1172
MlyI GAGTC 4 cut(s) 767, 1454, 1618, 1701
MmeI TCCRAC 2 cut(s) 904, 1711
Mox20I TGGCCA 1 cut(s) 1172
MscI TGGCCA 1 cut(s) 1172
MseI TTAA 4 cut(s) 600, 957, 1046, 1725
Msp20I TGGCCA 1 cut(s) 1172
MspA1I CMGCKG 3 cut(s) 253, 310, 466
MspCI CTTAAG 1 cut(s) 1724
MspI CCGG 3 cut(s) 245, 291, 1716
MspR9I CCNGG 7 cut(s) 245, 382, 400, 1002, 1065, 1401, 1648
MunI CAATTG 1 cut(s) 1128
Mva1269I GAATGC 1 cut(s) 1683
MvaI CCWGG 6 cut(s) 382, 400, 1002, 1065, 1401, 1648
MvnI CGCG 3 cut(s) 303, 310, 517
MwoI GCNNNNNNNGC 9 cut(s) 170, 259, 287, 307, 313, 469, 695, 701, 704
NciI CCSGG 1 cut(s) 245
NdeII GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
NlaIII CATG 4 cut(s) 1103, 1193, 1475, 1523
NlaIV GGNNCC 3 cut(s) 35, 326, 856
NmuCI GTSAC 2 cut(s) 124, 1579
NspI RCATGY 1 cut(s) 1193
PceI AGGCCT 3 cut(s) 84, 316, 371
PciI ACATGT 1 cut(s) 1189
PcsI WCGNNNNNNNCGW 5 cut(s) 150, 153, 162, 486, 1183
PctI GAATGC 1 cut(s) 1683
PfeI GAWTC 1 cut(s) 119
PfoI TCCNGGA 1 cut(s) 1646
PinAI ACCGGT 1 cut(s) 1715
PleI GAGTC 4 cut(s) 766, 1454, 1618, 1700
PpsI GAGTC 4 cut(s) 766, 1454, 1618, 1700
PscI ACATGT 1 cut(s) 1189
PshBI ATTAAT 1 cut(s) 957
Psp124BI GAGCTC 1 cut(s) 950
Psp6I CCWGG 6 cut(s) 380, 398, 1000, 1063, 1399, 1646
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 6 cut(s) 380, 398, 1000, 1063, 1399, 1646
PspN4I GGNNCC 3 cut(s) 35, 326, 856
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 5 cut(s) 33, 34, 280, 324, 1718
PstI CTGCAG 2 cut(s) 756, 1248
PsuI RGATCY 2 cut(s) 610, 854
PvuII CAGCTG 1 cut(s) 466
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SacI GAGCTC 1 cut(s) 950
SacII CCGCGG 1 cut(s) 311
SalI GTCGAC 1 cut(s) 569
SaqAI TTAA 4 cut(s) 600, 957, 1046, 1725
Sau3AI GATC 7 cut(s) 544, 610, 854, 1132, 1216, 1516, 1639
Sau96I GGNCC 5 cut(s) 33, 34, 280, 324, 1718
SbfI CCTGCAGG 1 cut(s) 756
SchI GAGTC 4 cut(s) 767, 1454, 1618, 1701
ScrFI CCNGG 7 cut(s) 245, 382, 400, 1002, 1065, 1401, 1648
SdaI CCTGCAGG 1 cut(s) 756
SduI GDGCHC 3 cut(s) 37, 646, 950
SfaNI GCATC 2 cut(s) 502, 1411
SfcI CTRYAG 5 cut(s) 699, 708, 752, 1244, 1482
Sfr303I CCGCGG 1 cut(s) 311
SgrBI CCGCGG 1 cut(s) 311
SinI GGWCC 2 cut(s) 324, 1718
SmlI CTYRAG 5 cut(s) 404, 548, 1093, 1312, 1724
SmoI CTYRAG 5 cut(s) 404, 548, 1093, 1312, 1724
Sse8387I CCTGCAGG 1 cut(s) 756
Sse9I AATT 7 cut(s) 889, 954, 1128, 1179, 1271, 1350, 1537
SseBI AGGCCT 3 cut(s) 84, 316, 371
SsiI CCGC 7 cut(s) 178, 253, 308, 310, 472, 1202, 1345
SspI AATATT 2 cut(s) 650, 880
SspMI CTAG 2 cut(s) 224, 900
SstI GAGCTC 1 cut(s) 950
StuI AGGCCT 3 cut(s) 84, 316, 371
StyD4I CCNGG 7 cut(s) 243, 380, 398, 1000, 1063, 1399, 1646
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 2 cut(s) 1585, 1667
TasI AATT 7 cut(s) 889, 954, 1128, 1179, 1271, 1350, 1537
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 4 cut(s) 600, 957, 1046, 1725
Tru9I TTAA 4 cut(s) 600, 957, 1046, 1725
TscAI CASTG 3 cut(s) 865, 1588, 1672
TseFI GTSAC 2 cut(s) 124, 1579
Tsp45I GTSAC 2 cut(s) 124, 1579
TspDTI ATGAA 7 cut(s) 375, 1116, 1254, 1344, 1488, 1506, 1530
TspGWI ACGGA 3 cut(s) 112, 309, 644
TspRI CASTG 3 cut(s) 865, 1588, 1672
Vha464I CTTAAG 1 cut(s) 1724
VpaK11BI GGWCC 2 cut(s) 324, 1718
VspI ATTAAT 1 cut(s) 957
XapI RAATTY 4 cut(s) 889, 1179, 1271, 1350
XceI RCATGY 1 cut(s) 1193
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 2 cut(s) 224, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.