Rmu_sc0000070.1_g000049

Large subunit GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000070.1
OG0010426
Physical Location & Seq
Forward (+)
147739 .. 148469
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000070.1_g000049.1.cds

Sequence Viewer

Length: 522 bp
atgccgccgaagaacgagaaggcgaagctaaggagagccctggttcggcagcacaaccagttgattcagcagaccaaagagaagggcatcatgtacagaaagatgcagaagaaggttctacaatccgtcacggaggtcagcgacatcgacgccgttattgaattgggattgtttccaagggacgggagtgagataaccccgccggagcaaaggagggagcagcagaggaaggaggaggccttgcacgccggtagtctccaggtgccacgtagaccgccgtggactccagagatgtcggtggaagagctcgatgacaatgagaggcaggccttcttggtctggcgcagaagcctcgccaggctggaggagaatgacaaacttgttctcactccttttgagaagaatttggacatctggaggcagctgtggcgtgtcgttgagcgtagcgatttacttgtgatggttgttgatgcccgggatcctctattctaccgctgccctgatcttgaggtttgcagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005783 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010154 GO:0010467 GO:0010646 GO:0010817 GO:0012505 GO:0015030 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016072 GO:0016462 GO:0016604 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0022414 GO:0022613 GO:0023051 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042254 GO:0042886 GO:0043021 GO:0043022 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046626 GO:0046907 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051049 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0090069 GO:0090070 GO:0090304 GO:0097159 GO:0097367 GO:0099402 GO:1900076 GO:1901265 GO:1901360 GO:1901363 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

173

Amino Acids

20.48

Weight (kDa)

7.77

Isoelectric Point (pI)

68.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 262
AccI GTMKAC 1 cut(s) 271
AciI CCGC 4 cut(s) 5, 200, 275, 493
AclWI GGATC 2 cut(s) 473, 486
AcsI RAATTY 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 45, 182
AgsI TTSAA 1 cut(s) 161
AjnI CCWGG 3 cut(s) 39, 258, 356
AluBI AGCT 3 cut(s) 28, 307, 424
AluI AGCT 3 cut(s) 28, 307, 424
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 2 cut(s) 473, 486
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 2 cut(s) 237, 327
ApeKI GCWGC 4 cut(s) 49, 220, 421, 495
ApoI RAATTY 1 cut(s) 403
AspLEI GCGC 1 cut(s) 345
AsuC2I CCSGG 2 cut(s) 475, 476
AvaI CYCGRG 1 cut(s) 474
BamHI GGATCC 1 cut(s) 478
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 2 cut(s) 40, 309
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 4 cut(s) 61, 232, 433, 482
BccI CCATC 1 cut(s) 454
BceAI ACGGC 2 cut(s) 137, 262
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 3 cut(s) 41, 260, 358
BcnI CCSGG 2 cut(s) 475, 476
BcoDI GTCTC 1 cut(s) 260
BisI GCNGC 5 cut(s) 5, 50, 221, 422, 496
BlsI GCNGC 5 cut(s) 6, 51, 222, 423, 497
Bme1390I CCNGG 5 cut(s) 41, 260, 358, 475, 476
BmeT110I CYCGRG 1 cut(s) 474
BmiI GGNNCC 2 cut(s) 264, 480
BmrFI CCNGG 5 cut(s) 41, 260, 358, 475, 476
BmsI GCATC 3 cut(s) 93, 96, 460
BpmI CTGGAG 4 cut(s) 242, 270, 383, 436
Bpu10I CCTNAGC 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 475, 476
BsaAI YACGTR 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 4 cut(s) 39, 176, 278, 474
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 182
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 58
BseBI CCWGG 3 cut(s) 41, 260, 358
BseDI CCNNGG 4 cut(s) 39, 176, 278, 474
BseLI CCNNNNNNNGG 2 cut(s) 45, 182
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 248, 380
BseXI GCAGC 4 cut(s) 61, 232, 433, 482
BshFI GGCC 2 cut(s) 239, 329
BshNI GGYRCC 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 3 cut(s) 203, 249, 475
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 2 cut(s) 45, 182
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 2 cut(s) 239, 329
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 40, 309
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 2 cut(s) 478, 502
BspACI CCGC 4 cut(s) 5, 200, 275, 493
BspANI GGCC 2 cut(s) 239, 329
BspLI GGNNCC 2 cut(s) 264, 480
BspPI GGATC 2 cut(s) 473, 486
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 1 cut(s) 58
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 4 cut(s) 39, 176, 278, 474
BssMI GATC 2 cut(s) 478, 502
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 41, 260, 358
Bst6I CTCTTC 1 cut(s) 297
BstACI GRCGYC 1 cut(s) 150
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 246, 327
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 278
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 2 cut(s) 481, 505
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 2 cut(s) 478, 502
BstMWI GCNNNNNNNGC 2 cut(s) 245, 427
BstNI CCWGG 3 cut(s) 41, 260, 358
BstSCI CCNGG 5 cut(s) 39, 258, 356, 473, 474
BstV1I GCAGC 4 cut(s) 61, 232, 433, 482
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 2 cut(s) 239, 329
BtgI CCRYGG 1 cut(s) 278
Cac8I GCNNGC 2 cut(s) 246, 327
CfoI GCGC 1 cut(s) 345
Cfr10I RCCGGY 1 cut(s) 248
Cfr9I CCCGGG 1 cut(s) 474
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 94
CviAII CATG 1 cut(s) 91
CviJI RGCY 8 cut(s) 28, 38, 239, 307, 329, 351, 361, 424
CviKI_1 RGCY 8 cut(s) 28, 38, 239, 307, 329, 351, 361, 424
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 29
DpnI GATC 2 cut(s) 480, 504
DpnII GATC 2 cut(s) 478, 502
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 176
Eco147I AGGCCT 2 cut(s) 239, 329
Eco24I GRGCYC 2 cut(s) 40, 309
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 474
EcoICRI GAGCTC 1 cut(s) 307
EcoRII CCWGG 3 cut(s) 39, 258, 356
EcoT14I CCWWGG 1 cut(s) 176
EcoT38I GRGCYC 2 cut(s) 40, 309
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 1 cut(s) 94
FaiI YATR 1 cut(s) 92
FaqI GGGAC 1 cut(s) 194
FatI CATG 1 cut(s) 90
FauI CCCGC 1 cut(s) 207
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 5 cut(s) 5, 50, 221, 422, 496
FriOI GRGCYC 2 cut(s) 40, 309
Fsp4HI GCNGC 5 cut(s) 5, 50, 221, 422, 496
GlaI GCGC 1 cut(s) 344
GluI GCNGC 5 cut(s) 5, 50, 221, 422, 496
GsuI CTGGAG 4 cut(s) 242, 270, 383, 436
HaeIII GGCC 2 cut(s) 239, 329
HapII CCGG 3 cut(s) 203, 249, 475
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HinfI GANTC 2 cut(s) 64, 283
HpaII CCGG 3 cut(s) 203, 249, 475
Hpy166II GTNNAC 2 cut(s) 272, 282
Hpy188III TCNNGA 3 cut(s) 287, 415, 506
Hpy8I GTNNAC 2 cut(s) 272, 282
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 13, 76, 106, 223, 340
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 3 cut(s) 106, 244, 516
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 427
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 343
Kzo9I GATC 2 cut(s) 478, 502
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 205, 217
Lsp1109I GCAGC 4 cut(s) 61, 232, 433, 482
LweI GCATC 3 cut(s) 93, 96, 460
MaeII ACGT 1 cut(s) 268
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 480, 504
MboI GATC 2 cut(s) 478, 502
MboII GAAGA 4 cut(s) 22, 121, 314, 412
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 2 cut(s) 40, 309
MluCI AATT 2 cut(s) 161, 403
MlyI GAGTC 1 cut(s) 277
MseI TTAA 1 cut(s) 520
MspA1I CMGCKG 2 cut(s) 424, 495
MspI CCGG 3 cut(s) 203, 249, 475
MspR9I CCNGG 5 cut(s) 41, 260, 358, 475, 476
MvaI CCWGG 3 cut(s) 41, 260, 358
MwoI GCNNNNNNNGC 2 cut(s) 245, 427
NciI CCSGG 2 cut(s) 475, 476
NdeII GATC 2 cut(s) 478, 502
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 2 cut(s) 264, 480
NmuCI GTSAC 1 cut(s) 127
PceI AGGCCT 2 cut(s) 239, 329
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 64
PkrI GCNGC 5 cut(s) 6, 51, 222, 423, 497
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 269
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 3 cut(s) 39, 258, 356
PspGI CCWGG 3 cut(s) 39, 258, 356
PspN4I GGNNCC 2 cut(s) 264, 480
PsuI RGATCY 1 cut(s) 478
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SacI GAGCTC 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 520
SatI GCNGC 5 cut(s) 5, 50, 221, 422, 496
Sau3AI GATC 2 cut(s) 478, 502
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 5 cut(s) 41, 260, 358, 475, 476
SduI GDGCHC 2 cut(s) 40, 309
SetI ASST 8 cut(s) 30, 117, 138, 264, 271, 309, 426, 513
SfaNI GCATC 3 cut(s) 93, 96, 460
SmaI CCCGGG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 2 cut(s) 161, 403
SseBI AGGCCT 2 cut(s) 239, 329
SsiI CCGC 4 cut(s) 5, 200, 275, 493
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 2 cut(s) 239, 329
StyD4I CCNGG 5 cut(s) 39, 258, 356, 473, 474
StyI CCWWGG 1 cut(s) 176
TaiI ACGT 1 cut(s) 271
TaqI TCGA 2 cut(s) 147, 309
TasI AATT 2 cut(s) 161, 403
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 1 cut(s) 520
Tru9I TTAA 1 cut(s) 520
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 4 cut(s) 49, 220, 421, 495
Tsp45I GTSAC 1 cut(s) 127
TspGWI ACGGA 2 cut(s) 115, 146
TspMI CCCGGG 1 cut(s) 474
XapI RAATTY 1 cut(s) 403
XmaI CCCGGG 1 cut(s) 474
XmiI GTMKAC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.