pycom05g02530

Large subunit GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010426
Physical Location & Seq
Forward (+)
3030325 .. 3032125
1801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02530.5

Sequence Viewer

Length: 930 bp
ATGGGTAAGAACGAGAAGACGGGGCTGGGTAGGGCCCTTGTGAGACAGCATAACCAACTTGTTCAGCAGACGAAAGAGAAGGGCCTGATGTACAAGAGGCAGCAGAAGAAGGTTCTCGAATCCGTCACCGAAGTCAGCGACATCGACGCCATCGTCGAAAAAGCCGACGAAGCCGACCGCCTCTTCTCCCTCGACCGCCCTGCCCCTAACCTCCTCATCGACCTAAGAGATGGGAATGAGATAACCCCGGAGCAGCGGAGGGAGCAGAAGAGGAAAGAGGAGGCCCTGCACGCCGGAAGTCTGCGCGTGCCGCGGAGGCCTCCGTGGACCCCTCAAATGTCTGTGGAGGAGCTGGATAACAATGAAAGACAGGCCTTCTTGACCTGGCGCAGAAGCCTTGCCAGGCTTGAGGAGAACGACAAGCTTCTCCTTACTCCTTTCGAGAAGAACCTCGATATTTGGAGGCAGCTGTGGCGGGTTGTCGAGCGTAGCGATTTGCTTGTGATGGTTGTTGATGCGCGAGACCCTCTATTCTATCGCTGCCCTGATCTTGAGGTTTATGCACGAGAGGTCGACAAGCACAAAAGGACAATGCTTCTTGTTAACAAAGCAGATCTCTTACCTTTATCTGTTAGGGAGAAGTGGGCACAATATTTCCGTTCGCAAGGTATTCTCTTTGTGTTTTGGTCGGCTAAAGCTGCTACAGCAGCTACAGAAGGGAAAAGTCTCAGTTCCTCGTGGACAGAAAATAGCCTGCAGGAGTCAGAAGACCCTGATACAAAAATATATGGGAGGATTGAGCTTTTGGCTCGTTTACAATCTGAGGCAGAAGAGATAGTCAAACTGAGGAAATCAGGATCCAGTGGTTCTCGAAGTGGAAATGTTGTTGGAAATTCAGCTTCTAGTAATGTAGTTGTGGGATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005783 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010154 GO:0010467 GO:0010646 GO:0010817 GO:0012505 GO:0015030 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016072 GO:0016462 GO:0016604 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0022414 GO:0022613 GO:0023051 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042254 GO:0042886 GO:0043021 GO:0043022 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046626 GO:0046907 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051049 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0090069 GO:0090070 GO:0090304 GO:0097159 GO:0097367 GO:0099402 GO:1900076 GO:1901265 GO:1901360 GO:1901363 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

310

Amino Acids

35.46

Weight (kDa)

8.82

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 573
AccII CGCG 3 cut(s) 306, 313, 520
AciI CCGC 6 cut(s) 178, 196, 256, 311, 313, 475
AclWI GGATC 2 cut(s) 852, 865
AcsI RAATTY 1 cut(s) 892
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 92
AjnI CCWGG 2 cut(s) 383, 401
AluBI AGCT 7 cut(s) 352, 424, 469, 698, 710, 802, 899
AluI AGCT 7 cut(s) 352, 424, 469, 698, 710, 802, 899
Alw26I GTCTC 3 cut(s) 37, 516, 731
AlwI GGATC 2 cut(s) 852, 865
AoxI GGCC 5 cut(s) 33, 82, 282, 317, 372
ApaI GGGCCC 1 cut(s) 37
ApeKI GCWGC 6 cut(s) 100, 253, 466, 540, 698, 707
ApoI RAATTY 1 cut(s) 892
AspLEI GCGC 3 cut(s) 306, 390, 520
AspS9I GGNCC 5 cut(s) 33, 34, 82, 283, 327
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 327
BaeGI GKGCMC 2 cut(s) 37, 649
BamHI GGATCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 37
BauI CACGAG 2 cut(s) 564, 736
BbsI GAAGAC 2 cut(s) 23, 774
BbvI GCAGC 6 cut(s) 112, 265, 478, 527, 685, 719
BccI CCATC 3 cut(s) 158, 224, 499
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 2 cut(s) 385, 403
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 37, 516, 731
BfaI CTAG 1 cut(s) 903
BfmI CTRYAG 3 cut(s) 702, 711, 755
BglI GCCNNNNNGGC 1 cut(s) 316
BglII AGATCT 1 cut(s) 613
BisI GCNGC 7 cut(s) 101, 254, 311, 467, 541, 699, 708
BlsI GCNGC 7 cut(s) 102, 255, 312, 468, 542, 700, 709
Bme1390I CCNGG 3 cut(s) 248, 385, 403
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 5 cut(s) 33, 34, 82, 283, 327
BmiI GGNNCC 3 cut(s) 35, 329, 859
BmrFI CCNGG 3 cut(s) 248, 385, 403
BmsI GCATC 1 cut(s) 505
BpiI GAAGAC 2 cut(s) 23, 774
BpuEI CTTGAG 2 cut(s) 428, 572
BpuMI CCSGG 1 cut(s) 248
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 246, 311, 323
Bse1I ACTGG 1 cut(s) 861
BseBI CCWGG 2 cut(s) 385, 403
BseDI CCNNGG 3 cut(s) 246, 311, 323
BseMII CTCAG 3 cut(s) 742, 813, 836
BseNI ACTGG 1 cut(s) 861
BseRI GAGGAG 4 cut(s) 203, 293, 362, 425
BseSI GKGCMC 2 cut(s) 37, 649
BseXI GCAGC 6 cut(s) 112, 265, 478, 527, 685, 719
BseYI CCCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 3 cut(s) 306, 313, 520
Bsh1285I CGRYCG 2 cut(s) 178, 196
BshFI GGCC 5 cut(s) 35, 84, 284, 319, 374
BsiEI CGRYCG 2 cut(s) 178, 196
BsiSI CCGG 2 cut(s) 248, 294
BsmAI GTCTC 3 cut(s) 37, 516, 731
BsnI GGCC 5 cut(s) 35, 84, 284, 319, 374
Bso31I GGTCTC 1 cut(s) 516
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 37, 649
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 3 cut(s) 547, 613, 857
BspACI CCGC 6 cut(s) 178, 196, 256, 311, 313, 475
BspANI GGCC 5 cut(s) 35, 84, 284, 319, 374
BspCNI CTCAG 3 cut(s) 741, 814, 837
BspFNI CGCG 3 cut(s) 306, 313, 520
BspLI GGNNCC 3 cut(s) 35, 329, 859
BspMAI CTGCAG 1 cut(s) 759
BspPI GGATC 2 cut(s) 852, 865
BspTNI GGTCTC 1 cut(s) 516
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 1 cut(s) 861
BssECI CCNNGG 3 cut(s) 246, 311, 323
BssMI GATC 3 cut(s) 547, 613, 857
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 2 cut(s) 564, 736
Bst2BI CACGAG 2 cut(s) 564, 736
Bst2UI CCWGG 2 cut(s) 385, 403
Bst6I CTCTTC 3 cut(s) 188, 263, 825
BstACI GRCGYC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 291, 308, 755
BstDEI CTNAG 4 cut(s) 224, 728, 822, 845
BstDSI CCRYGG 2 cut(s) 311, 323
BstFNI CGCG 3 cut(s) 306, 313, 520
BstHHI GCGC 3 cut(s) 306, 390, 520
BstKTI GATC 3 cut(s) 550, 616, 860
BstMAI GTCTC 3 cut(s) 37, 516, 731
BstMBI GATC 3 cut(s) 547, 613, 857
BstMCI CGRYCG 2 cut(s) 178, 196
BstMWI GCNNNNNNNGC 9 cut(s) 170, 262, 290, 310, 316, 472, 698, 704, 707
BstNI CCWGG 2 cut(s) 385, 403
BstSCI CCNGG 3 cut(s) 246, 383, 401
BstSFI CTRYAG 3 cut(s) 702, 711, 755
BstSLI GKGCMC 2 cut(s) 37, 649
BstUI CGCG 3 cut(s) 306, 313, 520
BstV1I GCAGC 6 cut(s) 112, 265, 478, 527, 685, 719
BstV2I GAAGAC 2 cut(s) 23, 774
BstX2I RGATCY 2 cut(s) 613, 857
BstYI RGATCY 2 cut(s) 613, 857
BsuRI GGCC 5 cut(s) 35, 84, 284, 319, 374
BtgI CCRYGG 2 cut(s) 311, 323
BtsIMutI CAGTG 1 cut(s) 868
Cac8I GCNNGC 3 cut(s) 291, 308, 755
CfoI GCGC 3 cut(s) 306, 390, 520
Cfr13I GGNCC 5 cut(s) 33, 34, 82, 283, 327
Cfr42I CCGCGG 1 cut(s) 314
CseI GACGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 91
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 4 cut(s) 224, 728, 822, 845
DpnI GATC 3 cut(s) 549, 615, 859
DpnII GATC 3 cut(s) 547, 613, 857
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 3 cut(s) 188, 263, 825
EarI CTCTTC 3 cut(s) 188, 263, 825
Eco147I AGGCCT 2 cut(s) 319, 374
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 327
EcoO109I RGGNCCY 4 cut(s) 33, 34, 82, 283
EcoRII CCWGG 2 cut(s) 383, 401
EcoT38I GRGCYC 1 cut(s) 37
FaiI YATR 4 cut(s) 51, 561, 787, 789
FauI CCCGC 1 cut(s) 468
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 7 cut(s) 101, 254, 311, 467, 541, 699, 708
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 7 cut(s) 101, 254, 311, 467, 541, 699, 708
FspBI CTAG 1 cut(s) 903
GlaI GCGC 3 cut(s) 305, 389, 519
GluI GCNGC 7 cut(s) 101, 254, 311, 467, 541, 699, 708
GsaI CCCAGC 1 cut(s) 29
HaeIII GGCC 5 cut(s) 35, 84, 284, 319, 374
HapII CCGG 2 cut(s) 248, 294
HgaI GACGC 1 cut(s) 155
HhaI GCGC 3 cut(s) 306, 390, 520
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 3 cut(s) 304, 388, 518
HinP1I GCGC 3 cut(s) 304, 388, 518
HincII GTYRAC 2 cut(s) 574, 604
HindII GTYRAC 2 cut(s) 574, 604
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 2 cut(s) 119, 761
HpaI GTTAAC 1 cut(s) 604
HpaII CCGG 2 cut(s) 248, 294
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 5 cut(s) 327, 574, 604, 741, 815
Hpy188I TCNGA 2 cut(s) 766, 823
Hpy188III TCNNGA 6 cut(s) 116, 379, 442, 551, 855, 870
Hpy8I GTNNAC 5 cut(s) 327, 574, 604, 741, 815
Hpy99I CGWCG 3 cut(s) 149, 158, 170
HpyAV CCTTC 4 cut(s) 73, 103, 385, 710
HpyCH4V TGCA 3 cut(s) 289, 563, 757
HpyF10VI GCNNNNNNNGC 9 cut(s) 170, 262, 290, 310, 316, 472, 698, 704, 707
HpyF3I CTNAG 4 cut(s) 224, 728, 822, 845
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 3 cut(s) 304, 388, 518
KspAI GTTAAC 1 cut(s) 604
KspI CCGCGG 1 cut(s) 314
Kzo9I GATC 3 cut(s) 547, 613, 857
LmnI GCTCC 3 cut(s) 250, 262, 349
Lsp1109I GCAGC 6 cut(s) 112, 265, 478, 527, 685, 719
LweI GCATC 1 cut(s) 505
MaeI CTAG 1 cut(s) 903
MaeIII GTNAC 1 cut(s) 124
MalI GATC 3 cut(s) 549, 615, 859
MboI GATC 3 cut(s) 547, 613, 857
MboII GAAGA 7 cut(s) 28, 118, 175, 280, 457, 779, 842
MflI RGATCY 2 cut(s) 613, 857
MhlI GDGCHC 2 cut(s) 37, 649
MluCI AATT 1 cut(s) 892
MlyI GAGTC 1 cut(s) 770
MmeI TCCRAC 1 cut(s) 868
MseI TTAA 1 cut(s) 603
MspA1I CMGCKG 3 cut(s) 256, 313, 469
MspI CCGG 2 cut(s) 248, 294
MspR9I CCNGG 3 cut(s) 248, 385, 403
MvaI CCWGG 2 cut(s) 385, 403
MvnI CGCG 3 cut(s) 306, 313, 520
MwoI GCNNNNNNNGC 9 cut(s) 170, 262, 290, 310, 316, 472, 698, 704, 707
NciI CCSGG 1 cut(s) 248
NdeII GATC 3 cut(s) 547, 613, 857
NlaIV GGNNCC 3 cut(s) 35, 329, 859
NmuCI GTSAC 1 cut(s) 124
PceI AGGCCT 2 cut(s) 319, 374
PcsI WCGNNNNNNNCGW 4 cut(s) 150, 153, 162, 489
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 7 cut(s) 102, 255, 312, 468, 542, 700, 709
PleI GAGTC 1 cut(s) 769
PpsI GAGTC 1 cut(s) 769
Psp6I CCWGG 2 cut(s) 383, 401
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 383, 401
PspN4I GGNNCC 3 cut(s) 35, 329, 859
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 5 cut(s) 33, 34, 82, 283, 327
PstI CTGCAG 1 cut(s) 759
PsuI RGATCY 2 cut(s) 613, 857
PvuII CAGCTG 1 cut(s) 469
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SacII CCGCGG 1 cut(s) 314
SalI GTCGAC 1 cut(s) 572
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 7 cut(s) 101, 254, 311, 467, 541, 699, 708
Sau3AI GATC 3 cut(s) 547, 613, 857
Sau96I GGNCC 5 cut(s) 33, 34, 82, 283, 327
SbfI CCTGCAGG 1 cut(s) 759
SchI GAGTC 1 cut(s) 770
ScrFI CCNGG 3 cut(s) 248, 385, 403
SdaI CCTGCAGG 1 cut(s) 759
SduI GDGCHC 2 cut(s) 37, 649
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 3 cut(s) 702, 711, 755
Sfr303I CCGCGG 1 cut(s) 314
SgrBI CCGCGG 1 cut(s) 314
SinI GGWCC 1 cut(s) 327
SmlI CTYRAG 2 cut(s) 407, 551
SmoI CTYRAG 2 cut(s) 407, 551
Sse8387I CCTGCAGG 1 cut(s) 759
Sse9I AATT 1 cut(s) 892
SseBI AGGCCT 2 cut(s) 319, 374
SsiI CCGC 6 cut(s) 178, 196, 256, 311, 313, 475
SspI AATATT 1 cut(s) 653
SspMI CTAG 1 cut(s) 903
StuI AGGCCT 2 cut(s) 319, 374
StyD4I CCNGG 3 cut(s) 246, 383, 401
TasI AATT 1 cut(s) 892
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 313
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TscAI CASTG 1 cut(s) 868
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 6 cut(s) 100, 253, 466, 540, 698, 707
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 378
TspGWI ACGGA 3 cut(s) 112, 312, 647
TspRI CASTG 1 cut(s) 868
VpaK11BI GGWCC 1 cut(s) 327
XapI RAATTY 1 cut(s) 892
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.