MD10G1037500.v1.1

Large subunit GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010426
Physical Location & Seq
Forward (+)
4933159 .. 4948777
15619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1037500.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGGTAAGAACGAGAAGACGGGTCTGGGTAGGGCTCTGGTGAGACAGCACAACCAGCTGGTTCAGCAAACGAAAGAGAAAGGCCTGATGTACAAGAGGCAGCAGAAGAAGGTTCTCGAATCCGTCACTGAAGTCAGCGACATCGACGCCATCGTCGAACAAGCTGACGAGGCCGACCGCCTCTTCTCCCTCGACCACCCTGCCCCTAACCTCCTCATCGACCTAGATGGGAATGAGATAACCCCGGAGCAGGGGAGGGAGCAGAAGAGGAAAGAGGAGGCCCTGCACGCCGGAAGTCTGCGGGTGCCGCGGAGGCCTGCGTGGACCCCTCAAATGTCTGTGGAGGAGCTGGATAACAATGAAAGGCAAGCCTTCTTGACTTGGCGCAGAAGCCTTGCCAGGCTTGAGGAGAACGACAAGCTTCTCCTTACTCCTTTCGAGAAGAACCTCGATATTTGGAGGCAGCTATGGCGGGTTGTGGAGCGTAGCGATTTGCTTGTGATGGTTGTTGATGCGCGAGACCCGCTATTCTATCGCTGCCCTGATCTTGAGGTGAAGCTTTGGTGTGAAGCTTTGTGGGTTTATGCACGAGAGGTCGACAAGCACAAAAGGACAATGCTTCTTGTTAACAAAGCAGATCTCTTACCTTTATCTGTTAGAGAGAAATGGGCCAATTATTTCTGTTCGCAAGATATTCTCTTTGTGTTTTGGTCGGCTAAAGCCGCTACAGCAGCTACAGAAGGGAAAAATCTCAGTTCCCCATGGACAGAAGATAACCTGCACGAGTCAGAAGACCCTGATACTAAAATATACGGGAGGATTGAGCTATTGGCTCGTTTACAATCTGAGGCTGAAGAAATAGTCAGACTGAGGAAATCAGGATCCAGTGATTCTCGAAGTGGAAGTGTTGTTGGAAATTCAGCTTCCAGTAATGTAGTTGTGGGATTTGTTGGATATCCAAATGTGGGAAAGAGCTCGACTATTAATGCCTTGGTAGGCCAGAAGAAGACAGGTGTTACCTCTACTCCTGGGAAGACAAAGCATTTCCAAACATTGATTATGTCTGATGAGTTAATGCTATGTGATTGCCCTGGATTAGTCTTTCCATCGTTTTCAAGCTCCAGACATGAGATGATTGCTTCTGGTGTATTGCCAATTGATCGAATGACCGAGAATAGGGAGGCTGTGCAGGTAGTGGCCAATCGAGTTCCAAGACATGTTATTGAAGCGGTTTACAGGATTGATCTGCCCAAACCCAAATCCTATGAACTGCAGTCGCGACCACCTCTGGCAGCAGAATTTTTGAGGGCCTATTGTGCCTCTCGTGGTTATGTTGCCTCTAGTGGACTTCCTGATGAAACAAAAGCTGCCCGCCAAATTTTGAAGGATTACATTGATGGGAAACTGCCCCATTATCAAATGCCCCCTGGAATGACTGCTGAGGAAGATGATGTGGGAAACGGCTTACCTGAACATCATAAGTCAGGCTCCTCTGATGATGAAAACTCTGAAGATGATGAAGGTGAAGACGAGCCGGAGCTTGATCATGCCCTGGAAGACCTCAATTCATTTGACATAGCTAATGGGCTTGCTACCAAGAAGAAAGTCGCTGTCAGGAAGCCCACTGCACCTCATAAACAACACAAGAAGACTCAGAGGAAAAAGGATCGCTCCTGGAGGGTAGGCAATGATGGTGGAGATGGAATGCCAGTTGTAAGAGTGTTTCAGAAACCAGCGAACACCGGTCCTGTTAAGGTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005783 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010154 GO:0010467 GO:0010646 GO:0010817 GO:0012505 GO:0015030 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016072 GO:0016462 GO:0016604 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0022414 GO:0022613 GO:0023051 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042254 GO:0042886 GO:0043021 GO:0043022 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046626 GO:0046907 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051049 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0090069 GO:0090070 GO:0090304 GO:0097159 GO:0097367 GO:0099402 GO:1900076 GO:1901265 GO:1901360 GO:1901363 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

588

Amino Acids

66.0

Weight (kDa)

6.22

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 314 - 385 2.2e-15 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
Acc36I ACCTGC 2 cut(s) 786, 1180
AccB1I GGYRCC 1 cut(s) 304
AccI GTMKAC 1 cut(s) 597
AccII CGCG 3 cut(s) 310, 517, 1279
AciI CCGC 9 cut(s) 178, 301, 308, 310, 472, 524, 723, 1229, 1372
AclWI GGATC 3 cut(s) 876, 889, 1674
AcoI YGGCCR 1 cut(s) 1197
AcsI RAATTY 3 cut(s) 916, 1298, 1377
AcuI CTGAAG 3 cut(s) 150, 873, 1530
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 4 cut(s) 250, 251, 965, 1176
AflIII ACRYGT 1 cut(s) 1216
AgeI ACCGGT 1 cut(s) 1742
AgsI TTSAA 3 cut(s) 1116, 1226, 1384
AjnI CCWGG 6 cut(s) 398, 1027, 1090, 1426, 1551, 1673
Alw21I GWGCWC 1 cut(s) 977
Alw26I GTCTC 2 cut(s) 37, 513
AlwI GGATC 3 cut(s) 876, 889, 1674
AoxI GGCC 8 cut(s) 82, 171, 279, 314, 669, 997, 1197, 1308
ApeKI GCWGC 6 cut(s) 100, 463, 537, 731, 1292, 1367
ApoI RAATTY 3 cut(s) 916, 1298, 1377
AseI ATTAAT 1 cut(s) 984
AsiGI ACCGGT 1 cut(s) 1742
AspLEI GCGC 2 cut(s) 387, 517
AspS9I GGNCC 5 cut(s) 280, 324, 669, 1308, 1745
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 3 cut(s) 52, 565, 1535
AvaII GGWCC 2 cut(s) 324, 1745
BalI TGGCCA 1 cut(s) 1199
BamHI GGATCC 1 cut(s) 881
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 2 cut(s) 37, 977
BauI CACGAG 3 cut(s) 588, 782, 1323
BbsI GAAGAC 7 cut(s) 23, 798, 1013, 1040, 1533, 1563, 1655
Bbv12I GWGCWC 1 cut(s) 977
BbvCI CCTCAGC 1 cut(s) 1440
BbvI GCAGC 6 cut(s) 112, 475, 524, 743, 1304, 1354
BccI CCATC 7 cut(s) 158, 221, 496, 1114, 1391, 1685, 1694
BceAI ACGGC 1 cut(s) 1477
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 6 cut(s) 400, 1029, 1092, 1428, 1553, 1675
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 37, 513
BfaI CTAG 2 cut(s) 224, 1341
BfmI CTRYAG 3 cut(s) 726, 735, 1271
BfuAI ACCTGC 2 cut(s) 786, 1180
BglI GCCNNNNNGGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 637
BisI GCNGC 8 cut(s) 101, 308, 464, 538, 723, 732, 1293, 1368
BlsI GCNGC 8 cut(s) 102, 309, 465, 539, 724, 733, 1294, 1369
Bme1390I CCNGG 7 cut(s) 245, 400, 1029, 1092, 1428, 1553, 1675
Bme18I GGWCC 2 cut(s) 324, 1745
BmgT120I GGNCC 5 cut(s) 280, 324, 669, 1308, 1745
BmiI GGNNCC 4 cut(s) 306, 326, 883, 1489
BmrFI CCNGG 7 cut(s) 245, 400, 1029, 1092, 1428, 1553, 1675
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 7 cut(s) 23, 798, 1013, 1040, 1533, 1563, 1655
BpmI CTGGAG 2 cut(s) 1105, 1696
Bpu10I CCTNAGC 1 cut(s) 1440
BpuEI CTTGAG 2 cut(s) 425, 569
BpuMI CCSGG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 513
BsaJI CCNNGG 8 cut(s) 243, 308, 761, 990, 1028, 1090, 1426, 1551
BsaWI WCCGGW 1 cut(s) 1742
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 251, 965, 1176
Bse118I RCCGGY 1 cut(s) 1742
Bse1I ACTGG 3 cut(s) 885, 927, 1709
Bse3DI GCAATG 1 cut(s) 1693
BseBI CCWGG 6 cut(s) 400, 1029, 1092, 1428, 1553, 1675
BseDI CCNNGG 8 cut(s) 243, 308, 761, 990, 1028, 1090, 1426, 1551
BseLI CCNNNNNNNGG 4 cut(s) 250, 251, 965, 1176
BseMI GCAATG 1 cut(s) 1693
BseMII CTCAG 5 cut(s) 766, 837, 860, 1431, 1667
BseNI ACTGG 3 cut(s) 885, 927, 1709
BseRI GAGGAG 5 cut(s) 203, 290, 359, 422, 1480
BseXI GCAGC 6 cut(s) 112, 475, 524, 743, 1304, 1354
BsgI GTGCAG 4 cut(s) 269, 764, 1208, 1611
Bsh1236I CGCG 3 cut(s) 310, 517, 1279
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 8 cut(s) 84, 173, 281, 316, 671, 999, 1199, 1310
BshNI GGYRCC 1 cut(s) 304
BshTI ACCGGT 1 cut(s) 1742
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 977
BsiSI CCGG 4 cut(s) 245, 291, 1535, 1743
BslI CCNNNNNNNGG 4 cut(s) 250, 251, 965, 1176
BsmAI GTCTC 2 cut(s) 37, 513
BsmI GAATGC 1 cut(s) 1710
BsnI GGCC 8 cut(s) 84, 173, 281, 316, 671, 999, 1199, 1310
Bso31I GGTCTC 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 37, 977
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
Bsp19I CCATGG 1 cut(s) 761
Bsp68I TCGCGA 1 cut(s) 1279
BspACI CCGC 9 cut(s) 178, 301, 308, 310, 472, 524, 723, 1229, 1372
BspANI GGCC 8 cut(s) 84, 173, 281, 316, 671, 999, 1199, 1310
BspCNI CTCAG 5 cut(s) 765, 838, 861, 1432, 1666
BspFNI CGCG 3 cut(s) 310, 517, 1279
BspLI GGNNCC 4 cut(s) 306, 326, 883, 1489
BspMAI CTGCAG 1 cut(s) 1275
BspMI ACCTGC 2 cut(s) 786, 1180
BspPI GGATC 3 cut(s) 876, 889, 1674
BspT107I GGYRCC 1 cut(s) 304
BspTNI GGTCTC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 1693
BsrFI RCCGGY 1 cut(s) 1742
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 3 cut(s) 885, 927, 1709
BssAI RCCGGY 1 cut(s) 1742
BssECI CCNNGG 8 cut(s) 243, 308, 761, 990, 1028, 1090, 1426, 1551
BssMI GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 3 cut(s) 588, 782, 1323
BssT1I CCWWGG 2 cut(s) 761, 990
Bst2BI CACGAG 3 cut(s) 588, 782, 1323
Bst2UI CCWGG 6 cut(s) 400, 1029, 1092, 1428, 1553, 1675
Bst6I CTCTTC 2 cut(s) 188, 260
BstACI GRCGYC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 5 cut(s) 288, 318, 369, 1372, 1590
BstDEI CTNAG 5 cut(s) 752, 846, 869, 1440, 1653
BstDSI CCRYGG 2 cut(s) 308, 761
BstFNI CGCG 3 cut(s) 310, 517, 1279
BstHHI GCGC 2 cut(s) 387, 517
BstKTI GATC 7 cut(s) 547, 640, 884, 1162, 1246, 1546, 1669
BstMAI GTCTC 2 cut(s) 37, 513
BstMBI GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
BstMCI CGRYCG 1 cut(s) 178
BstNI CCWGG 6 cut(s) 400, 1029, 1092, 1428, 1553, 1675
BstNSI RCATGY 1 cut(s) 1220
BstSCI CCNGG 7 cut(s) 243, 398, 1027, 1090, 1426, 1551, 1673
BstSFI CTRYAG 3 cut(s) 726, 735, 1271
BstUI CGCG 3 cut(s) 310, 517, 1279
BstV1I GCAGC 6 cut(s) 112, 475, 524, 743, 1304, 1354
BstV2I GAAGAC 7 cut(s) 23, 798, 1013, 1040, 1533, 1563, 1655
BstX2I RGATCY 2 cut(s) 637, 881
BstYI RGATCY 2 cut(s) 637, 881
BsuRI GGCC 8 cut(s) 84, 173, 281, 316, 671, 999, 1199, 1310
BtgI CCRYGG 2 cut(s) 308, 761
BtsI GCAGTG 1 cut(s) 1623
BtsIMutI CAGTG 3 cut(s) 126, 892, 1623
BtuMI TCGCGA 1 cut(s) 1279
BveI ACCTGC 2 cut(s) 786, 1180
Cac8I GCNNGC 5 cut(s) 288, 318, 369, 1372, 1590
CfoI GCGC 2 cut(s) 387, 517
Cfr10I RCCGGY 1 cut(s) 1742
Cfr13I GGNCC 5 cut(s) 280, 324, 669, 1308, 1745
Cfr42I CCGCGG 1 cut(s) 311
CseI GACGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 91
CspAI ACCGGT 1 cut(s) 1742
CspCI CAANNNNNGTGG 2 cut(s) 1675, 1710
CviAII CATG 4 cut(s) 762, 1127, 1217, 1547
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 5 cut(s) 752, 846, 869, 1440, 1653
DpnI GATC 7 cut(s) 546, 639, 883, 1161, 1245, 1545, 1668
DpnII GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 1197
Eam1104I CTCTTC 2 cut(s) 188, 260
EarI CTCTTC 2 cut(s) 188, 260
Ecl136II GAGCTC 1 cut(s) 975
Eco130I CCWWGG 2 cut(s) 761, 990
Eco147I AGGCCT 2 cut(s) 84, 316
Eco24I GRGCYC 2 cut(s) 37, 977
Eco31I GGTCTC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 956
Eco47I GGWCC 2 cut(s) 324, 1745
Eco53kI GAGCTC 1 cut(s) 975
Eco57I CTGAAG 3 cut(s) 150, 873, 1530
EcoICRI GAGCTC 1 cut(s) 975
EcoO109I RGGNCCY 2 cut(s) 280, 1308
EcoRII CCWGG 6 cut(s) 398, 1027, 1090, 1426, 1551, 1673
EcoRV GATATC 1 cut(s) 956
EcoT14I CCWWGG 2 cut(s) 761, 990
EcoT38I GRGCYC 2 cut(s) 37, 977
ErhI CCWWGG 2 cut(s) 761, 990
FaeI CATG 4 cut(s) 765, 1130, 1220, 1550
FatI CATG 4 cut(s) 761, 1126, 1216, 1546
FauI CCCGC 4 cut(s) 294, 465, 531, 1379
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 8 cut(s) 101, 308, 464, 538, 723, 732, 1293, 1368
FriOI GRGCYC 2 cut(s) 37, 977
Fsp4HI GCNGC 8 cut(s) 101, 308, 464, 538, 723, 732, 1293, 1368
FspBI CTAG 2 cut(s) 224, 1341
GlaI GCGC 2 cut(s) 386, 516
GluI GCNGC 8 cut(s) 101, 308, 464, 538, 723, 732, 1293, 1368
GsuI CTGGAG 2 cut(s) 1105, 1696
HaeIII GGCC 8 cut(s) 84, 173, 281, 316, 671, 999, 1199, 1310
HapII CCGG 4 cut(s) 245, 291, 1535, 1743
HgaI GACGC 1 cut(s) 155
HhaI GCGC 2 cut(s) 387, 517
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 765, 1130, 1220, 1550
Hin6I GCGC 2 cut(s) 385, 515
HinP1I GCGC 2 cut(s) 385, 515
HincII GTYRAC 2 cut(s) 598, 628
HindII GTYRAC 2 cut(s) 598, 628
HindIII AAGCTT 3 cut(s) 419, 557, 570
HinfI GANTC 4 cut(s) 119, 785, 890, 1651
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 4 cut(s) 245, 291, 1535, 1743
HphI GGTGA 3 cut(s) 52, 565, 1535
Hpy166II GTNNAC 6 cut(s) 324, 598, 628, 839, 1234, 1346
Hpy188I TCNGA 8 cut(s) 790, 847, 866, 1066, 1495, 1510, 1656, 1728
Hpy8I GTNNAC 6 cut(s) 324, 598, 628, 839, 1234, 1346
Hpy99I CGWCG 2 cut(s) 149, 158
HpyAV CCTTC 5 cut(s) 103, 382, 734, 1378, 1514
HpyCH4V TGCA 6 cut(s) 286, 587, 781, 1189, 1273, 1628
HpyF3I CTNAG 5 cut(s) 752, 846, 869, 1440, 1653
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 765, 1130, 1220, 1550
HspAI GCGC 2 cut(s) 385, 515
Ksp22I TGATCA 1 cut(s) 1543
KspAI GTTAAC 1 cut(s) 628
KspI CCGCGG 1 cut(s) 311
Kzo9I GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
LmnI GCTCC 8 cut(s) 247, 259, 346, 481, 1124, 1493, 1537, 1676
Lsp1109I GCAGC 6 cut(s) 112, 475, 524, 743, 1304, 1354
LweI GCATC 1 cut(s) 502
MaeI CTAG 2 cut(s) 224, 1341
MaeIII GTNAC 2 cut(s) 124, 1015
MalI GATC 7 cut(s) 546, 639, 883, 1161, 1245, 1545, 1668
MboI GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 2 cut(s) 637, 881
MhlI GDGCHC 2 cut(s) 37, 977
MlsI TGGCCA 1 cut(s) 1199
MluCI AATT 6 cut(s) 673, 916, 1155, 1298, 1377, 1564
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 2 cut(s) 794, 1645
MmeI TCCRAC 3 cut(s) 892, 931, 1738
Mox20I TGGCCA 1 cut(s) 1199
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 4 cut(s) 627, 984, 1073, 1752
Msp20I TGGCCA 1 cut(s) 1199
MspA1I CMGCKG 2 cut(s) 58, 310
MspI CCGG 4 cut(s) 245, 291, 1535, 1743
MspR9I CCNGG 7 cut(s) 245, 400, 1029, 1092, 1428, 1553, 1675
MunI CAATTG 1 cut(s) 1155
Mva1269I GAATGC 1 cut(s) 1710
MvaI CCWGG 6 cut(s) 400, 1029, 1092, 1428, 1553, 1675
MvnI CGCG 3 cut(s) 310, 517, 1279
NciI CCSGG 1 cut(s) 245
NcoI CCATGG 1 cut(s) 761
NdeII GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
NlaIII CATG 4 cut(s) 765, 1130, 1220, 1550
NlaIV GGNNCC 4 cut(s) 306, 326, 883, 1489
NmuCI GTSAC 1 cut(s) 124
NruI TCGCGA 1 cut(s) 1279
NspI RCATGY 1 cut(s) 1220
PceI AGGCCT 2 cut(s) 84, 316
PciI ACATGT 1 cut(s) 1216
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 153
PctI GAATGC 1 cut(s) 1710
PfeI GAWTC 2 cut(s) 119, 890
PfoI TCCNGGA 1 cut(s) 1673
PinAI ACCGGT 1 cut(s) 1742
PkrI GCNGC 8 cut(s) 102, 309, 465, 539, 724, 733, 1294, 1369
PleI GAGTC 2 cut(s) 793, 1645
PpsI GAGTC 2 cut(s) 793, 1645
PscI ACATGT 1 cut(s) 1216
PshBI ATTAAT 1 cut(s) 984
Psp124BI GAGCTC 1 cut(s) 977
Psp6I CCWGG 6 cut(s) 398, 1027, 1090, 1426, 1551, 1673
PspGI CCWGG 6 cut(s) 398, 1027, 1090, 1426, 1551, 1673
PspN4I GGNNCC 4 cut(s) 306, 326, 883, 1489
PspPI GGNCC 5 cut(s) 280, 324, 669, 1308, 1745
PstI CTGCAG 1 cut(s) 1275
PsuI RGATCY 2 cut(s) 637, 881
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 1279
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SacI GAGCTC 1 cut(s) 977
SacII CCGCGG 1 cut(s) 311
SalI GTCGAC 1 cut(s) 596
SaqAI TTAA 4 cut(s) 627, 984, 1073, 1752
SatI GCNGC 8 cut(s) 101, 308, 464, 538, 723, 732, 1293, 1368
Sau3AI GATC 7 cut(s) 544, 637, 881, 1159, 1243, 1543, 1666
Sau96I GGNCC 5 cut(s) 280, 324, 669, 1308, 1745
SchI GAGTC 2 cut(s) 794, 1645
ScrFI CCNGG 7 cut(s) 245, 400, 1029, 1092, 1428, 1553, 1675
SduI GDGCHC 2 cut(s) 37, 977
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 3 cut(s) 726, 735, 1271
Sfr303I CCGCGG 1 cut(s) 311
SgrBI CCGCGG 1 cut(s) 311
SinI GGWCC 2 cut(s) 324, 1745
SmlI CTYRAG 2 cut(s) 404, 548
SmoI CTYRAG 2 cut(s) 404, 548
Sse9I AATT 6 cut(s) 673, 916, 1155, 1298, 1377, 1564
SseBI AGGCCT 2 cut(s) 84, 316
SsiI CCGC 9 cut(s) 178, 301, 308, 310, 472, 524, 723, 1229, 1372
SspMI CTAG 2 cut(s) 224, 1341
SstI GAGCTC 1 cut(s) 977
StuI AGGCCT 2 cut(s) 84, 316
StyD4I CCNGG 7 cut(s) 243, 398, 1027, 1090, 1426, 1551, 1673
StyI CCWWGG 2 cut(s) 761, 990
TaqII GACCGA 1 cut(s) 1184
TasI AATT 6 cut(s) 673, 916, 1155, 1298, 1377, 1564
TatI WGTACW 1 cut(s) 90
TauI GCSGC 2 cut(s) 310, 725
TfiI GAWTC 2 cut(s) 119, 890
Tru1I TTAA 4 cut(s) 627, 984, 1073, 1752
Tru9I TTAA 4 cut(s) 627, 984, 1073, 1752
TscAI CASTG 3 cut(s) 133, 892, 1630
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 6 cut(s) 100, 463, 537, 731, 1292, 1367
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 6 cut(s) 375, 1281, 1371, 1515, 1533, 1557
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 3 cut(s) 133, 892, 1630
VpaK11BI GGWCC 2 cut(s) 324, 1745
VspI ATTAAT 1 cut(s) 984
XapI RAATTY 3 cut(s) 916, 1298, 1377
XceI RCATGY 1 cut(s) 1220
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 2 cut(s) 224, 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.