Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010005

Physical Location & Seq

Genomic Coordinates Reverse (-)

5723870 .. 5730276

Gene Structure 6407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1035500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGGAGGAGTATCAAGAACTCTACTCACTTTCTCTTTCTCCTCAAACCTCTTCGCCTTATCTCGTCTCCCGGCCGCCAAATGCTTCTCTCTCATCAAACCCACCAGGGTTTTAACAGGGTTTAGGCCTCTTTGCACCGCCACTGCCACAACCACCACCGCCAGCCCCGCCAGCGCGGTTGATTTCGAAGACGAAGAAGTCCAACCCCTGAAGCACTCGTTGCTTCTTGAGAGGCTGCGGCTGAGACACCTCAAGAGTAATGCCAAGCCCCAAGCCAGAACCGGTGGCGGAAATTCAATTGGGGATGCCCAGAGGCAGAGCGATGAGGGGTTGGGGAGGTCCGAGAGTAGGAAGAAGAGGGAAGTTGGGAGTTTTGGGGAGTTGGGTGTGAGCGAGGAGGTCATGGCGGCGGTGCGAGAGATGGGGATTGAGGTTCCCACTGAAATCCAGTGCATTGGGATACCGGCGGTGTTGGAAGGGAAGACTGTGGTCTTAGGTTCACACACTGGCTCCGGCAAGACTCTGGCTTACATGTTGCCTCTTGCCCAGTTGTTGAGAAGGGATGAAGCTGAAAATGGTATTCAAATGAAGCCCAGGCGTCCTAGAGCTGTTGTGCTATGCCCCACAAGGGAGCTGTCTGAGCAGGTTTTTCGTGTTTCCAAGTTTGTAAGTCACCATGCAAGGTTCAGGTCTACTATGGTAAGCGGTGGTGGTCGGTTGAGACCTCAGGAGGATTCATTGAATAACCCAATTGAAATGGTAGTGGGTACCCCTGGAAGGGTTCTCCAACATATTGAGGATGGCAACTTGGTTTATGGTGACATCAAATACTTAGTATTGGATGAGGCAGATACCATGTTTGATCGTGGCTTTGGTCCAGATATACGCAAGTTTCTAGTCCCATTAAAACATCGTGCATCAAAACCTGGAGGGCAAGGGTTCCAAACTGTTTTGGTCTCAGCAACAATGACAAAGGCAGTGCAAAATCTGATCGATGAGGAGTTCCAAGGAATATTACACTTGCGCACATCCTCACTGCATAAGAAGATTGCATCTGCTCGCCATGATTTTGTTAAGATTTCAGGTTCTGAGAACAAGCTGGAATCACTTCTACAGGTTCTGGAGCCAAGCTTAGCAAAGGGAAATAGAGTAATGGTGTTCTGTAACACATTAAGTTCCAGCCGTGCTGTGGATCACTTCCTCAATGAAAACCAGATCTCTACTGTCAATTATCATGGTGAGGTGCCAGCAGAACAAAGGGTTGAGAACCTCAAAAGGTTTAAGAGCAACGATGGAGATTGCCCTACATTGGTCTGCACAGACCTGGCTGCAAGGGGACTGGACTTGGATGTAGACCATGTTATCATGTTTGATTTTCCTTTGAACTCTATTGACTACCTTCATCGTACAGGAAGAACCGCTCGTATGGGTGCAAAAGGGAAAGTAACGAGTTTGGTTGCTAAGAAGAACCTTATGTTAGCAAACCGAATTGAGGAAGCAATAAAGAAGAATGAAAGCTTAGAATCTCTCAGCGTAGATAGTGTCAAGAGGGACATTGCCCGCTTGCGAATTACTCCACAGAATGGAAAATTGGTAAGAGTCTCAAATCAGAAAAACAAGAGCAGGCCAGCATTTGCACCTGCAAAATCTGCAAAACCATCTTTTCAGTCATCGAAATCAGTAAAGCCTTCCAGAGCAAGCAGCTCCAAAAGAGCTCCTTCCAATGCAAGCAACTCAAGAAAAGCTCCTTCCAATGCAAGCAGCTCGAGAAAAGCATCTTTTAGTGAGAAGAGGCAAACAGAAGGCAGAAGATCCAGTACAGTTAAATCAACACCCTCGAAGTTAAGTGTTGTTGGGTTTAGGGGTCGGGCTTCTTGGTCTAATAAGAGAGAATCGTTGGCGTCAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0010501 GO:0016043 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019843 GO:0022607 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034622 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0065003 GO:0070013 GO:0070035 GO:0070180 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0110102 GO:0140098 GO:1901259 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

637

Amino Acids

69.66

Weight (kDa)

10.08

Isoelectric Point (pI)

46.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	147 - 329	1.4e-37	DEAD/DEAH box helicase
Helicase_C	PF00271	366 - 477	2.5e-27	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09730
fragaria_vesca	FvH4_2g12870 FvH4_2g12870 FvH4_2g12870 FvH4_2g12870
malus_domestica	MD05G1035500.v1.1 MD10G1037800.v1.1
prunus_persica	Prupe.8G043300_v2.0.a1
pyrus_communis	pycom05g02600 pycom10g02570
rosa_chinensis	RchiOBHm_Chr6g0273971
rosa_laevigata	RLG00000013588
rosa_multiflora	Rmu_sc0000045.1_g000081 Rmu_sc0013374.1_g000007
rosa_roxburghii	Rroxscaffold_7G00194840
rosa_rugosa	Rorug06G0077900
rosa_samantha	Rh6AG193500 Rh6BG197100 Rh6CG197000 Rh6DG188900
rosa_wichuraiana	Rw6G016870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1648
AasI	GACNNNNNNGTC	1 cut(s)	197
Acc16I	TGCGCA	1 cut(s)	1025
Acc36I	ACCTGC	2 cut(s)	634, 1648
Acc65I	GGTACC	1 cut(s)	767
AccB1I	GGYRCC	2 cut(s)	767, 1243
AccB7I	CCANNNNNTGG	1 cut(s)	1583
AccBSI	CCGCTC	1 cut(s)	1421
AccI	GTMKAC	2 cut(s)	692, 1353
AccII	CGCG	1 cut(s)	176
AclWI	GGATC	2 cut(s)	1200, 1806
AcoI	YGGCCR	1 cut(s)	72
AcsI	RAATTY	1 cut(s)	292
AcuI	CTGAAG	1 cut(s)	230
AcyI	GRCGYC	2 cut(s)	598, 1901
AfaI	GTAC	3 cut(s)	769, 1408, 1819
AfiI	CCNNNNNNNGG	8 cut(s)	348, 628, 777, 778, 1189, 1309, 1492, 1583
AflIII	ACRYGT	1 cut(s)	531
AgeI	ACCGGT	1 cut(s)	281
AgsI	TTSAA	5 cut(s)	297, 584, 742, 755, 1384
AjnI	CCWGG	5 cut(s)	104, 593, 772, 925, 1323
AjuI	GAANNNNNNNTTGG	2 cut(s)	1574, 1606
Alw21I	GWGCWC	1 cut(s)	1717
Alw26I	GTCTC	5 cut(s)	71, 238, 715, 961, 1606
AlwI	GGATC	2 cut(s)	1200, 1806
AlwNI	CAGNNNCTG	2 cut(s)	1088, 1120
Ama87I	CYCGRG	1 cut(s)	1765
AoxI	GGCC	3 cut(s)	72, 125, 1625
ApeKI	GCWGC	4 cut(s)	235, 1328, 1701, 1761
ApoI	RAATTY	1 cut(s)	292
AsiGI	ACCGGT	1 cut(s)	281
Asp700I	GAANNNNTTC	1 cut(s)	1107
Asp718I	GGTACC	1 cut(s)	767
AspLEI	GCGC	2 cut(s)	176, 1026
AspS9I	GGNCC	2 cut(s)	339, 875
AsuC2I	CCSGG	1 cut(s)	71
AsuHPI	GGTGA	3 cut(s)	665, 830, 1250
AsuII	TTCGAA	1 cut(s)	186
AvaI	CYCGRG	1 cut(s)	1765
AvaII	GGWCC	2 cut(s)	339, 875
AxyI	CCTNAGG	1 cut(s)	726
BanI	GGYRCC	2 cut(s)	767, 1243
BanII	GRGCYC	1 cut(s)	1717
BarI	GAAGNNNNNNTAC	2 cut(s)	1801, 1833
BbsI	GAAGAC	2 cut(s)	195, 488
Bbv12I	GWGCWC	1 cut(s)	1717
BbvI	GCAGC	4 cut(s)	222, 1315, 1713, 1773
BccI	CCATC	4 cut(s)	415, 794, 1286, 1666
BceAI	ACGGC	1 cut(s)	1167
BcgI	CGANNNNNNTGC	4 cut(s)	632, 666, 1746, 1780
BciT130I	CCWGG	5 cut(s)	106, 595, 774, 927, 1325
BciVI	GTATCC	1 cut(s)	453
BcnI	CCSGG	1 cut(s)	71
BcoDI	GTCTC	5 cut(s)	71, 238, 715, 961, 1606
BfaI	CTAG	2 cut(s)	603, 896
BfmI	CTRYAG	1 cut(s)	1112
BfuAI	ACCTGC	2 cut(s)	634, 1648
BfuI	GTATCC	1 cut(s)	453
BglII	AGATCT	1 cut(s)	1215
BisI	GCNGC	7 cut(s)	75, 236, 239, 408, 1329, 1702, 1762
BlpI	GCTNAGC	1 cut(s)	1132
BlsI	GCNGC	7 cut(s)	76, 237, 240, 409, 1330, 1703, 1763
Bme1390I	CCNGG	6 cut(s)	71, 106, 595, 774, 927, 1325
Bme18I	GGWCC	2 cut(s)	339, 875
BmeT110I	CYCGRG	1 cut(s)	1765
BmgT120I	GGNCC	2 cut(s)	339, 875
BmiI	GGNNCC	6 cut(s)	435, 511, 769, 941, 1125, 1245
BmrFI	CCNGG	6 cut(s)	71, 106, 595, 774, 927, 1325
BmrI	ACTGGG	1 cut(s)	541
BmsI	GCATC	4 cut(s)	295, 926, 1061, 1784
BmuI	ACTGGG	1 cut(s)	541
BoxI	GACNNNNGTC	1 cut(s)	488
BpiI	GAAGAC	2 cut(s)	195, 488
BpmI	CTGGAG	2 cut(s)	948, 1142
Bpu1102I	GCTNAGC	1 cut(s)	1132
Bpu14I	TTCGAA	1 cut(s)	186
BpuEI	CTTGAG	3 cut(s)	236, 248, 1720
BpuMI	CCSGG	1 cut(s)	71
Bsa29I	ATCGAT	1 cut(s)	993
BsaHI	GRCGYC	2 cut(s)	598, 1901
BsaI	GGTCTC	2 cut(s)	715, 961
BsaJI	CCNNGG	4 cut(s)	105, 593, 772, 1006
BsaWI	WCCGGW	1 cut(s)	281
BsaXI	ACNNNNNCTCC	2 cut(s)	494, 524
Bsc4I	CCNNNNNNNGG	8 cut(s)	348, 628, 777, 778, 1189, 1309, 1492, 1583
Bse118I	RCCGGY	2 cut(s)	281, 463
Bse1I	ACTGG	5 cut(s)	448, 511, 547, 1344, 1815
Bse21I	CCTNAGG	1 cut(s)	726
Bse3DI	GCAATG	1 cut(s)	1554
BseBI	CCWGG	5 cut(s)	106, 595, 774, 927, 1325
BseCI	ATCGAT	1 cut(s)	993
BseDI	CCNNGG	4 cut(s)	105, 593, 772, 1006
BseGI	GGATG	6 cut(s)	310, 568, 805, 847, 1028, 1354
BseLI	CCNNNNNNNGG	8 cut(s)	348, 628, 777, 778, 1189, 1309, 1492, 1583
BseMI	GCAATG	1 cut(s)	1554
BseMII	CTCAG	6 cut(s)	233, 630, 740, 972, 1080, 1543
BseNI	ACTGG	5 cut(s)	448, 511, 547, 1344, 1815
BseRI	GAGGAG	4 cut(s)	21, 31, 410, 1013
BseX3I	CGGCCG	1 cut(s)	72
BseXI	GCAGC	4 cut(s)	222, 1315, 1713, 1773
BsgI	GTGCAG	1 cut(s)	1300
Bsh1236I	CGCG	1 cut(s)	176
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	3 cut(s)	74, 127, 1627
BshNI	GGYRCC	2 cut(s)	767, 1243
BshTI	ACCGGT	1 cut(s)	281
BshVI	ATCGAT	1 cut(s)	993
BsiEI	CGRYCG	1 cut(s)	75
BsiHKAI	GWGCWC	1 cut(s)	1717
BsiHKCI	CYCGRG	1 cut(s)	1765
BsiSI	CCGG	4 cut(s)	71, 282, 464, 513
BslFI	GGGAC	3 cut(s)	884, 1350, 1565
BslI	CCNNNNNNNGG	8 cut(s)	348, 628, 777, 778, 1189, 1309, 1492, 1583
BsmAI	GTCTC	5 cut(s)	71, 238, 715, 961, 1606
BsmBI	CGTCTC	1 cut(s)	71
BsmFI	GGGAC	3 cut(s)	884, 1350, 1565
BsnI	GGCC	3 cut(s)	74, 127, 1627
Bso31I	GGTCTC	2 cut(s)	715, 961
BsoBI	CYCGRG	1 cut(s)	1765
Bsp119I	TTCGAA	1 cut(s)	186
Bsp1286I	GDGCHC	1 cut(s)	1717
Bsp143I	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
Bsp1720I	GCTNAGC	1 cut(s)	1132
BspANI	GGCC	3 cut(s)	74, 127, 1627
BspCNI	CTCAG	6 cut(s)	234, 631, 739, 971, 1081, 1542
BspDI	ATCGAT	1 cut(s)	993
BspFNI	CGCG	1 cut(s)	176
BspLI	GGNNCC	6 cut(s)	435, 511, 769, 941, 1125, 1245
BspMI	ACCTGC	2 cut(s)	634, 1648
BspPI	GGATC	2 cut(s)	1200, 1806
BspT104I	TTCGAA	1 cut(s)	186
BspT107I	GGYRCC	2 cut(s)	767, 1243
BspTNI	GGTCTC	2 cut(s)	715, 961
BsrBI	CCGCTC	1 cut(s)	1421
BsrDI	GCAATG	1 cut(s)	1554
BsrFI	RCCGGY	2 cut(s)	281, 463
BsrI	ACTGG	5 cut(s)	448, 511, 547, 1344, 1815
BssAI	RCCGGY	2 cut(s)	281, 463
BssECI	CCNNGG	4 cut(s)	105, 593, 772, 1006
BssMI	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
BssNI	GRCGYC	2 cut(s)	598, 1901
BssT1I	CCWWGG	1 cut(s)	1006
Bst2UI	CCWGG	5 cut(s)	106, 595, 774, 927, 1325
Bst4CI	ACNGT	4 cut(s)	487, 949, 1225, 1822
Bst6I	CTCTTC	3 cut(s)	56, 350, 1784
BstACI	GRCGYC	2 cut(s)	598, 1901
BstAPI	GCANNNNNTGC	2 cut(s)	220, 1649
BstBI	TTCGAA	1 cut(s)	186
BstF5I	GGATG	6 cut(s)	310, 568, 805, 847, 1028, 1354
BstFNI	CGCG	1 cut(s)	176
BstHHI	GCGC	2 cut(s)	176, 1026
BstKTI	GATC	5 cut(s)	865, 993, 1195, 1218, 1814
BstMAI	GTCTC	5 cut(s)	71, 238, 715, 961, 1606
BstMBI	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
BstMCI	CGRYCG	1 cut(s)	75
BstMWI	GCNNNNNNNGC	5 cut(s)	167, 171, 220, 640, 1649
BstNI	CCWGG	5 cut(s)	106, 595, 774, 927, 1325
BstNSI	RCATGY	1 cut(s)	535
BstPAI	GACNNNNGTC	1 cut(s)	488
BstSCI	CCNGG	6 cut(s)	69, 104, 593, 772, 925, 1323
BstSFI	CTRYAG	1 cut(s)	1112
BstUI	CGCG	1 cut(s)	176
BstV1I	GCAGC	4 cut(s)	222, 1315, 1713, 1773
BstV2I	GAAGAC	2 cut(s)	195, 488
BstX2I	RGATCY	2 cut(s)	1215, 1811
BstXI	CCANNNNNNTGG	1 cut(s)	455
BstYI	RGATCY	2 cut(s)	1215, 1811
BstZI	CGGCCG	1 cut(s)	72
Bsu15I	ATCGAT	1 cut(s)	993
Bsu36I	CCTNAGG	1 cut(s)	726
BsuI	GTATCC	1 cut(s)	453
BsuRI	GGCC	3 cut(s)	74, 127, 1627
BsuTUI	ATCGAT	1 cut(s)	993
BtgZI	GCGATG	1 cut(s)	336
BtsCI	GGATG	6 cut(s)	310, 568, 805, 847, 1028, 1354
BtsI	GCAGTG	3 cut(s)	141, 984, 1034
BtsIMutI	CAGTG	6 cut(s)	141, 438, 455, 504, 984, 1034
BveI	ACCTGC	2 cut(s)	634, 1648
CaiI	CAGNNNCTG	2 cut(s)	1088, 1120
CfoI	GCGC	2 cut(s)	176, 1026
Cfr10I	RCCGGY	2 cut(s)	281, 463
Cfr13I	GGNCC	2 cut(s)	339, 875
ClaI	ATCGAT	1 cut(s)	993
CseI	GACGC	2 cut(s)	587, 1890
Csp6I	GTAC	3 cut(s)	768, 1407, 1818
CspAI	ACCGGT	1 cut(s)	281
CviAII	CATG	8 cut(s)	403, 532, 677, 856, 1064, 1235, 1358, 1366
CviQI	GTAC	3 cut(s)	768, 1407, 1818
DpnI	GATC	5 cut(s)	864, 992, 1194, 1217, 1813
DpnII	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
DrdI	GACNNNNNNGTC	1 cut(s)	197
DseDI	GACNNNNNNGTC	1 cut(s)	197
EaeI	YGGCCR	1 cut(s)	72
EagI	CGGCCG	1 cut(s)	72
Eam1104I	CTCTTC	3 cut(s)	56, 350, 1784
EarI	CTCTTC	3 cut(s)	56, 350, 1784
EciI	GGCGGA	1 cut(s)	303
Ecl136II	GAGCTC	1 cut(s)	1715
EclXI	CGGCCG	1 cut(s)	72
Eco130I	CCWWGG	1 cut(s)	1006
Eco147I	AGGCCT	1 cut(s)	127
Eco24I	GRGCYC	1 cut(s)	1717
Eco31I	GGTCTC	2 cut(s)	715, 961
Eco47I	GGWCC	2 cut(s)	339, 875
Eco52I	CGGCCG	1 cut(s)	72
Eco53kI	GAGCTC	1 cut(s)	1715
Eco57I	CTGAAG	1 cut(s)	230
Eco81I	CCTNAGG	1 cut(s)	726
Eco88I	CYCGRG	1 cut(s)	1765
EcoICRI	GAGCTC	1 cut(s)	1715
EcoRII	CCWGG	5 cut(s)	104, 593, 772, 925, 1323
EcoT14I	CCWWGG	1 cut(s)	1006
EcoT38I	GRGCYC	1 cut(s)	1717
ErhI	CCWWGG	1 cut(s)	1006
Esp3I	CGTCTC	1 cut(s)	71
FaeI	CATG	8 cut(s)	406, 535, 680, 859, 1067, 1238, 1361, 1369
FalI	AAGNNNNNCTT	2 cut(s)	1702, 1734
FaqI	GGGAC	3 cut(s)	884, 1350, 1565
FatI	CATG	8 cut(s)	402, 531, 676, 855, 1063, 1234, 1357, 1365
FauI	CCCGC	2 cut(s)	175, 1568
FblI	GTMKAC	2 cut(s)	692, 1353
Fnu4HI	GCNGC	7 cut(s)	75, 236, 239, 408, 1329, 1702, 1762
FokI	GGATG	6 cut(s)	317, 575, 812, 854, 1015, 1361
FriOI	GRGCYC	1 cut(s)	1717
Fsp4HI	GCNGC	7 cut(s)	75, 236, 239, 408, 1329, 1702, 1762
FspBI	CTAG	2 cut(s)	603, 896
FspI	TGCGCA	1 cut(s)	1025
GlaI	GCGC	2 cut(s)	175, 1025
GluI	GCNGC	7 cut(s)	75, 236, 239, 408, 1329, 1702, 1762
GsuI	CTGGAG	2 cut(s)	948, 1142
HaeIII	GGCC	3 cut(s)	74, 127, 1627
HapII	CCGG	4 cut(s)	71, 282, 464, 513
HgaI	GACGC	2 cut(s)	587, 1890
HhaI	GCGC	2 cut(s)	176, 1026
Hin1I	GRCGYC	2 cut(s)	598, 1901
Hin1II	CATG	8 cut(s)	406, 535, 680, 859, 1067, 1238, 1361, 1369
Hin6I	GCGC	2 cut(s)	174, 1024
HinP1I	GCGC	2 cut(s)	174, 1024
HindIII	AAGCTT	2 cut(s)	1129, 1516
HinfI	GANTC	6 cut(s)	520, 734, 1103, 1523, 1599, 1892
HpaII	CCGG	4 cut(s)	71, 282, 464, 513
HphI	GGTGA	3 cut(s)	665, 830, 1250
Hpy166II	GTNNAC	3 cut(s)	500, 693, 1354
Hpy188I	TCNGA	5 cut(s)	343, 640, 990, 1090, 1611
Hpy8I	GTNNAC	3 cut(s)	500, 693, 1354
HpyAV	CCTTC	8 cut(s)	470, 552, 771, 1409, 1698, 1728, 1758, 1796
HpyCH4III	ACNGT	4 cut(s)	487, 949, 1225, 1822
HpyF10VI	GCNNNNNNNGC	5 cut(s)	167, 171, 220, 640, 1649
Hsp92I	GRCGYC	2 cut(s)	598, 1901
Hsp92II	CATG	8 cut(s)	406, 535, 680, 859, 1067, 1238, 1361, 1369
HspAI	GCGC	2 cut(s)	174, 1024
KpnI	GGTACC	1 cut(s)	771
Kzo9I	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
LmnI	GCTCC	6 cut(s)	515, 631, 1123, 1709, 1720, 1750
Lsp1109I	GCAGC	4 cut(s)	222, 1315, 1713, 1773
LweI	GCATC	4 cut(s)	295, 926, 1061, 1784
MaeI	CTAG	2 cut(s)	603, 896
MaeIII	GTNAC	4 cut(s)	671, 818, 1163, 1444
MalI	GATC	5 cut(s)	864, 992, 1194, 1217, 1813
MbiI	CCGCTC	1 cut(s)	1421
MboI	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
MfeI	CAATTG	2 cut(s)	297, 750
MflI	RGATCY	2 cut(s)	1215, 1811
MhlI	GDGCHC	1 cut(s)	1717
MluCI	AATT	7 cut(s)	292, 297, 750, 1228, 1488, 1569, 1589
MlyI	GAGTC	2 cut(s)	514, 1608
MmeI	TCCRAC	3 cut(s)	226, 453, 811
MroXI	GAANNNNTTC	1 cut(s)	1107
MseI	TTAA	7 cut(s)	113, 905, 1074, 1172, 1281, 1824, 1844
MspI	CCGG	4 cut(s)	71, 282, 464, 513
MspR9I	CCNGG	6 cut(s)	71, 106, 595, 774, 927, 1325
MunI	CAATTG	2 cut(s)	297, 750
MvaI	CCWGG	5 cut(s)	106, 595, 774, 927, 1325
MvnI	CGCG	1 cut(s)	176
MwoI	GCNNNNNNNGC	5 cut(s)	167, 171, 220, 640, 1649
NciI	CCSGG	1 cut(s)	71
NdeII	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
NlaIII	CATG	8 cut(s)	406, 535, 680, 859, 1067, 1238, 1361, 1369
NlaIV	GGNNCC	6 cut(s)	435, 511, 769, 941, 1125, 1245
NmuCI	GTSAC	2 cut(s)	671, 818
NsbI	TGCGCA	1 cut(s)	1025
NspI	RCATGY	1 cut(s)	535
NspV	TTCGAA	1 cut(s)	186
PaeR7I	CTCGAG	1 cut(s)	1765
PaqCI	CACCTGC	1 cut(s)	1648
PceI	AGGCCT	1 cut(s)	127
PciI	ACATGT	1 cut(s)	531
PdmI	GAANNNNTTC	1 cut(s)	1107
PfeI	GAWTC	4 cut(s)	734, 1103, 1523, 1892
PflMI	CCANNNNNTGG	1 cut(s)	1583
PinAI	ACCGGT	1 cut(s)	281
PkrI	GCNGC	7 cut(s)	76, 237, 240, 409, 1330, 1703, 1763
PleI	GAGTC	2 cut(s)	514, 1607
PpsI	GAGTC	2 cut(s)	514, 1607
PscI	ACATGT	1 cut(s)	531
PshAI	GACNNNNGTC	1 cut(s)	488
Psp124BI	GAGCTC	1 cut(s)	1717
Psp6I	CCWGG	5 cut(s)	104, 593, 772, 925, 1323
PspGI	CCWGG	5 cut(s)	104, 593, 772, 925, 1323
PspN4I	GGNNCC	6 cut(s)	435, 511, 769, 941, 1125, 1245
PspPI	GGNCC	2 cut(s)	339, 875
PstNI	CAGNNNCTG	2 cut(s)	1088, 1120
PsuI	RGATCY	2 cut(s)	1215, 1811
RsaI	GTAC	3 cut(s)	769, 1408, 1819
RsaNI	GTAC	3 cut(s)	768, 1407, 1818
SacI	GAGCTC	1 cut(s)	1717
SaqAI	TTAA	7 cut(s)	113, 905, 1074, 1172, 1281, 1824, 1844
SatI	GCNGC	7 cut(s)	75, 236, 239, 408, 1329, 1702, 1762
Sau3AI	GATC	5 cut(s)	862, 990, 1192, 1215, 1811
Sau96I	GGNCC	2 cut(s)	339, 875
SchI	GAGTC	2 cut(s)	514, 1608
ScrFI	CCNGG	6 cut(s)	71, 106, 595, 774, 927, 1325
SduI	GDGCHC	1 cut(s)	1717
SfaNI	GCATC	4 cut(s)	295, 926, 1061, 1784
SfcI	CTRYAG	1 cut(s)	1112
Sfr274I	CTCGAG	1 cut(s)	1765
SfuI	TTCGAA	1 cut(s)	186
SinI	GGWCC	2 cut(s)	339, 875
SlaI	CTCGAG	1 cut(s)	1765
SmlI	CTYRAG	4 cut(s)	227, 251, 1735, 1765
SmoI	CTYRAG	4 cut(s)	227, 251, 1735, 1765
Sse9I	AATT	7 cut(s)	292, 297, 750, 1228, 1488, 1569, 1589
SseBI	AGGCCT	1 cut(s)	127
SspI	AATATT	1 cut(s)	1014
SspMI	CTAG	2 cut(s)	603, 896
SstI	GAGCTC	1 cut(s)	1717
StuI	AGGCCT	1 cut(s)	127
StyD4I	CCNGG	6 cut(s)	69, 104, 593, 772, 925, 1323
StyI	CCWWGG	1 cut(s)	1006
TaaI	ACNGT	4 cut(s)	487, 949, 1225, 1822
TaqI	TCGA	5 cut(s)	186, 993, 1673, 1766, 1838
TasI	AATT	7 cut(s)	292, 297, 750, 1228, 1488, 1569, 1589
TatI	WGTACW	1 cut(s)	1817
TauI	GCSGC	3 cut(s)	77, 241, 410
TfiI	GAWTC	4 cut(s)	734, 1103, 1523, 1892
Tru1I	TTAA	7 cut(s)	113, 905, 1074, 1172, 1281, 1824, 1844
Tru9I	TTAA	7 cut(s)	113, 905, 1074, 1172, 1281, 1824, 1844
TscAI	CASTG	6 cut(s)	148, 445, 455, 511, 984, 1041
TseFI	GTSAC	2 cut(s)	671, 818
TseI	GCWGC	4 cut(s)	235, 1328, 1701, 1761
Tsp45I	GTSAC	2 cut(s)	671, 818
TspDTI	ATGAA	6 cut(s)	579, 602, 726, 1221, 1391, 1527
TspRI	CASTG	6 cut(s)	148, 445, 455, 511, 984, 1041
Van91I	CCANNNNNTGG	1 cut(s)	1583
VpaK11BI	GGWCC	2 cut(s)	339, 875
XapI	RAATTY	1 cut(s)	292
XceI	RCATGY	1 cut(s)	535
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1186
XhoI	CTCGAG	1 cut(s)	1765
XmiI	GTMKAC	2 cut(s)	692, 1353
XmnI	GAANNNNTTC	1 cut(s)	1107
XspI	CTAG	2 cut(s)	603, 896

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.