Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0010005

Physical Location & Seq

Genomic Coordinates Reverse (-)

36931628 .. 36938196

Gene Structure 6569 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00194840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGGGAGGAGCAGCAAAGACCCTTCTCACTCTGTCTCTCTCCTCAAATCTCTTCACTCTCTCACGTCTCCCCGGACCCGCCAGACGCTTCTCTCTCACAAAACTACCAAAACCCACCAGGGTTTTAACAGGGTTTAGGCCTCTTTGCACTGCCACTGCCACCGTTAGTCCCATTCAAGTTGAAGACCAAGACGGACTTCAACCCTTGAAGCATTCCGTGCTTCTTGAGAGGCTTAGGCTCAGGCACCTCAAGAACTCTACTAAACCACAAAACCCAACGAGCACAAAGTCGGTTGGGTATGGGGAGAGGCAGAGTGAGGATAGGTCAGGGAATAAGAAGAAGATTGAGGTTGAGAGTTTTGAGGAATTGGGTGTGAGTGAGGAGGTGTTGGAGGCTTTGAGAGAGATGGGCATTCAAGTTCCTACTGAAATTCAGTGCATTGGAATCCCAGCAGTGTTGGAAGGCAAGACTGTGGTCTTGGGTTCTCATACTGGGTCTGGCAAGACTTTGGCTTACATGTTGCCTCTTGCACAGCTGCTGAGAAGGGATGAGGCCCAAAATGGTATTATAATGAAGCCCAGGCGTCCCCGAGCTGTTGTGCTATGCCCCACTAGAGAGCTGTCTGAGCAGGTGTTTCGTGTTGCCAAGTTCATCAGTCATCATGCAAGATTCAGGTCTACTATGGTAAGCGGTGGTGGTAGGTTGAGACCCCAGGAGGATTCGTTGAATAACCCAATTGACATGGTAGTTGGGACCCCTGGAAGGATTCTTCAACATATTGAGGATGGCAACTTGGTTTATGGTGACATCAAATACTTGGTGTTGGATGAGGCAGATACTATGTTTGATCGTGGCTTTGGTCCAGACATTCGCAAGTTTCTTGCCCCATTAAAGAATCGGGCTTCGAAACCTGATGGCCAAGGATTCCAAACTGTTTTGGTCTCTGCCACAATGACAAAGGGAGTGCAAACTCTGATTGATGAGGAGTTCCAAGGAATACTACATTTGCGCACATCGTCACTGCATAAGAAGATTGCATCTGCTCGTCATGATTTCATCAAGCTTTCTGGTTCTGAGAACAAGATGGAAGCATTGCTGCAGGTTCTTGAGCCAAGTTTAGCAAAGGGAAACAGAGTAATGGTGTTCTGCAACACTTTAAATTCTAGCCGAGCTGTGGATCACTTTCTCAATGAAAATCAGATCTCGACTGCCAATTACCACGGTGAGGTGCCAGCAGAACAAAGGGTTGAAAACCTCAAAAAGTTCAAGAGTGATGATGGAGATTGCCCAACGTTGGTGTGCACAGACCTAGCTGCAAGGGGACTAGACTTGGATGTAGACCATGTTATCATGTTTGATTTTCCCTTGAATTCTATTGACTACCTTCATCGTACCGGAAGAACAGCTCGCATGGGTGCCAAAGGGAAAGTGACGAGTTTGGTTGCTAAGAAGAACCTTATGTTAGCCACTCGTATTGAGGAAGCGATAAAGAAGAATGAGAGCTTAGAATCGCTGAGTGTAGACAGTGTCAAGAGGGATGTTGCTCGTGCCCGCATTACTCCACAAAAAGCGAGCAATCCAAAATTTACAAGAGTCTCAAATCAGAGATCCAAGAGCAGACCATCGGGAACAAAATATGCGAAGACATCTGTTCAGGCATCAAAGTCGGTAAAGGCTGTAAAGCCTTCCAACACAAATAACTCTAAGAAATCTTTTTCTTCTGGGAAGAGGCCATCAGAAAGCAGAAGGTCAGCTGCAGTTAAATCATCAGCTTCAAAGTTGAGTGTTGTTGGGTTTAGGGGACGGACTTCTTGGTCTAATAAGAAAGAATCATCAGTGTCCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0010501 GO:0016043 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019843 GO:0022607 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034622 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0065003 GO:0070013 GO:0070035 GO:0070180 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0110102 GO:0140098 GO:1901259 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

615

Amino Acids

67.71

Weight (kDa)

9.97

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	142 - 324	4.4e-39	DEAD/DEAH box helicase
Helicase_C	PF00271	361 - 472	5.7e-28	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010005)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09730
fragaria_vesca	FvH4_2g12870 FvH4_2g12870 FvH4_2g12870 FvH4_2g12870
malus_domestica	MD05G1035500.v1.1 MD10G1037800.v1.1
prunus_persica	Prupe.8G043300_v2.0.a1
pyrus_communis	pycom05g02600 pycom10g02570
rosa_chinensis	RchiOBHm_Chr6g0273971
rosa_laevigata	RLG00000013588
rosa_multiflora	Rmu_sc0000045.1_g000081 Rmu_sc0013374.1_g000007
rosa_roxburghii	Rroxscaffold_7G00194840
rosa_rugosa	Rorug06G0077900
rosa_samantha	Rh6AG193500 Rh6BG197100 Rh6CG197000 Rh6DG188900
rosa_wichuraiana	Rw6G016870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	569
AarI	CACCTGC	1 cut(s)	619
AasI	GACNNNNNNGTC	1 cut(s)	1813
Acc16I	TGCGCA	1 cut(s)	1010
Acc36I	ACCTGC	2 cut(s)	619, 1090
AccB1I	GGYRCC	3 cut(s)	243, 1228, 1415
AccI	GTMKAC	3 cut(s)	677, 1338, 1521
AciI	CCGC	3 cut(s)	78, 690, 1552
AclI	AACGTT	1 cut(s)	1292
AclWI	GGATC	2 cut(s)	1185, 1602
AcoI	YGGCCR	1 cut(s)	916
AcsI	RAATTY	4 cut(s)	429, 1159, 1369, 1583
AcyI	GRCGYC	1 cut(s)	583
AfaI	GTAC	1 cut(s)	1393
AfiI	CCNNNNNNNGG	4 cut(s)	762, 1174, 1225, 1294
AflIII	ACRYGT	1 cut(s)	516
AjiI	CACGTC	1 cut(s)	65
AjnI	CCWGG	4 cut(s)	116, 578, 711, 757
AjuI	GAANNNNNNNTTGG	2 cut(s)	180, 212
Alw21I	GWGCWC	2 cut(s)	284, 1304
Alw26I	GTCTC	5 cut(s)	39, 71, 700, 946, 1600
Alw44I	GTGCAC	1 cut(s)	1300
AlwI	GGATC	2 cut(s)	1185, 1602
AlwNI	CAGNNNCTG	1 cut(s)	538
Ama87I	CYCGRG	1 cut(s)	588
AoxI	GGCC	4 cut(s)	137, 552, 916, 1730
ApaLI	GTGCAC	1 cut(s)	1300
ApeKI	GCWGC	5 cut(s)	11, 535, 1096, 1313, 1754
ApoI	RAATTY	4 cut(s)	429, 1159, 1369, 1583
AspLEI	GCGC	1 cut(s)	1011
AspS9I	GGNCC	4 cut(s)	74, 553, 753, 860
AsuC2I	CCSGG	1 cut(s)	72
AsuHPI	GGTGA	2 cut(s)	815, 1235
AsuII	TTCGAA	1 cut(s)	905
AvaI	CYCGRG	1 cut(s)	588
AvaII	GGWCC	3 cut(s)	74, 753, 860
BaeGI	GKGCMC	2 cut(s)	1304, 1552
BalI	TGGCCA	1 cut(s)	918
BanI	GGYRCC	3 cut(s)	243, 1228, 1415
BauI	CACGAG	1 cut(s)	1545
BbsI	GAAGAC	2 cut(s)	189, 1649
Bbv12I	GWGCWC	2 cut(s)	284, 1304
BbvI	GCAGC	5 cut(s)	23, 522, 1083, 1300, 1741
BccI	CCATC	7 cut(s)	400, 779, 908, 1078, 1271, 1630, 1741
BcgI	CGANNNNNNTGC	4 cut(s)	617, 651, 1647, 1681
BciT130I	CCWGG	4 cut(s)	118, 580, 713, 759
BcnI	CCSGG	1 cut(s)	72
BcoDI	GTCTC	5 cut(s)	39, 71, 700, 946, 1600
BfaI	CTAG	4 cut(s)	612, 1164, 1310, 1325
BfmI	CTRYAG	2 cut(s)	1097, 1755
BfuAI	ACCTGC	2 cut(s)	619, 1090
BglII	AGATCT	1 cut(s)	1200
BisI	GCNGC	5 cut(s)	12, 536, 1097, 1314, 1755
BlsI	GCNGC	5 cut(s)	13, 537, 1098, 1315, 1756
Bme1390I	CCNGG	5 cut(s)	72, 118, 580, 713, 759
Bme18I	GGWCC	3 cut(s)	74, 753, 860
BmeT110I	CYCGRG	1 cut(s)	588
BmgBI	CACGTC	1 cut(s)	65
BmgT120I	GGNCC	4 cut(s)	74, 553, 753, 860
BmiI	GGNNCC	6 cut(s)	76, 245, 754, 755, 1230, 1417
BmrFI	CCNGG	5 cut(s)	72, 118, 580, 713, 759
BmrI	ACTGGG	1 cut(s)	501
BmsI	GCATC	2 cut(s)	1046, 1667
BmuI	ACTGGG	1 cut(s)	501
BoxI	GACNNNNGTC	1 cut(s)	473
BpiI	GAAGAC	2 cut(s)	189, 1649
Bpu10I	CCTNAGC	2 cut(s)	233, 239
Bpu14I	TTCGAA	1 cut(s)	905
BpuEI	CTTGAG	3 cut(s)	233, 245, 1127
BpuMI	CCSGG	1 cut(s)	72
BsaHI	GRCGYC	1 cut(s)	583
BsaI	GGTCTC	2 cut(s)	700, 946
BsaJI	CCNNGG	8 cut(s)	70, 117, 578, 711, 757, 919, 991, 1219
BsaWI	WCCGGW	1 cut(s)	1394
BsaXI	ACNNNNNCTCC	2 cut(s)	707, 737
Bsc4I	CCNNNNNNNGG	4 cut(s)	762, 1174, 1225, 1294
Bse1I	ACTGG	1 cut(s)	496
Bse3DI	GCAATG	1 cut(s)	1091
BseBI	CCWGG	4 cut(s)	118, 580, 713, 759
BseDI	CCNNGG	8 cut(s)	70, 117, 578, 711, 757, 919, 991, 1219
BseGI	GGATG	5 cut(s)	553, 790, 832, 1339, 1543
BseLI	CCNNNNNNNGG	4 cut(s)	762, 1174, 1225, 1294
BseMI	GCAATG	1 cut(s)	1091
BseMII	CTCAG	5 cut(s)	253, 530, 615, 1065, 1505
BseNI	ACTGG	1 cut(s)	496
BseRI	GAGGAG	4 cut(s)	21, 31, 395, 998
BseSI	GKGCMC	2 cut(s)	1304, 1552
BseXI	GCAGC	5 cut(s)	23, 522, 1083, 1300, 1741
BseYI	CCCAGC	1 cut(s)	448
BshFI	GGCC	4 cut(s)	139, 554, 918, 1732
BshNI	GGYRCC	3 cut(s)	243, 1228, 1415
BsiHKAI	GWGCWC	2 cut(s)	284, 1304
BsiHKCI	CYCGRG	1 cut(s)	588
BsiSI	CCGG	2 cut(s)	72, 1395
BslFI	GGGAC	5 cut(s)	153, 570, 766, 1335, 1815
BslI	CCNNNNNNNGG	4 cut(s)	762, 1174, 1225, 1294
BsmAI	GTCTC	5 cut(s)	39, 71, 700, 946, 1600
BsmBI	CGTCTC	1 cut(s)	71
BsmFI	GGGAC	5 cut(s)	153, 570, 766, 1335, 1815
BsmI	GAATGC	2 cut(s)	211, 411
BsnI	GGCC	4 cut(s)	139, 554, 918, 1732
Bso31I	GGTCTC	2 cut(s)	700, 946
BsoBI	CYCGRG	1 cut(s)	588
Bsp119I	TTCGAA	1 cut(s)	905
Bsp1286I	GDGCHC	3 cut(s)	284, 1304, 1552
Bsp143I	GATC	4 cut(s)	847, 1177, 1200, 1607
BspACI	CCGC	3 cut(s)	78, 690, 1552
BspANI	GGCC	4 cut(s)	139, 554, 918, 1732
BspCNI	CTCAG	5 cut(s)	252, 531, 616, 1066, 1506
BspHI	TCATGA	1 cut(s)	1048
BspLI	GGNNCC	6 cut(s)	76, 245, 754, 755, 1230, 1417
BspMAI	CTGCAG	2 cut(s)	1101, 1759
BspMI	ACCTGC	2 cut(s)	619, 1090
BspPI	GGATC	2 cut(s)	1185, 1602
BspT104I	TTCGAA	1 cut(s)	905
BspT107I	GGYRCC	3 cut(s)	243, 1228, 1415
BspTNI	GGTCTC	2 cut(s)	700, 946
BsrDI	GCAATG	1 cut(s)	1091
BsrI	ACTGG	1 cut(s)	496
BssECI	CCNNGG	8 cut(s)	70, 117, 578, 711, 757, 919, 991, 1219
BssMI	GATC	4 cut(s)	847, 1177, 1200, 1607
BssNI	GRCGYC	1 cut(s)	583
BssSI	CACGAG	1 cut(s)	1545
BssT1I	CCWWGG	2 cut(s)	919, 991
Bst2BI	CACGAG	1 cut(s)	1545
Bst2UI	CCWGG	4 cut(s)	118, 580, 713, 759
Bst4CI	ACNGT	5 cut(s)	163, 472, 934, 1223, 1526
Bst6I	CTCTTC	2 cut(s)	56, 1721
BstACI	GRCGYC	1 cut(s)	583
BstAPI	GCANNNNNTGC	1 cut(s)	217
BstBI	TTCGAA	1 cut(s)	905
BstC8I	GCNNGC	4 cut(s)	1233, 1408, 1552, 1573
BstDEI	CTNAG	9 cut(s)	233, 239, 539, 624, 1074, 1446, 1504, 1514, 1704
BstDSI	CCRYGG	1 cut(s)	1219
BstF5I	GGATG	5 cut(s)	553, 790, 832, 1339, 1543
BstHHI	GCGC	1 cut(s)	1011
BstKTI	GATC	4 cut(s)	850, 1180, 1203, 1610
BstMAI	GTCTC	5 cut(s)	39, 71, 700, 946, 1600
BstMBI	GATC	4 cut(s)	847, 1177, 1200, 1607
BstMWI	GCNNNNNNNGC	2 cut(s)	217, 625
BstNI	CCWGG	4 cut(s)	118, 580, 713, 759
BstNSI	RCATGY	1 cut(s)	520
BstPAI	GACNNNNGTC	1 cut(s)	473
BstSCI	CCNGG	5 cut(s)	70, 116, 578, 711, 757
BstSFI	CTRYAG	2 cut(s)	1097, 1755
BstSLI	GKGCMC	2 cut(s)	1304, 1552
BstV1I	GCAGC	5 cut(s)	23, 522, 1083, 1300, 1741
BstV2I	GAAGAC	2 cut(s)	189, 1649
BstX2I	RGATCY	2 cut(s)	1200, 1607
BstYI	RGATCY	2 cut(s)	1200, 1607
BsuRI	GGCC	4 cut(s)	139, 554, 918, 1732
BtgI	CCRYGG	1 cut(s)	1219
BtrI	CACGTC	1 cut(s)	65
BtsCI	GGATG	5 cut(s)	553, 790, 832, 1339, 1543
BtsI	GCAGTG	4 cut(s)	147, 153, 459, 1019
BtsIMutI	CAGTG	7 cut(s)	147, 153, 440, 459, 1019, 1531, 1842
BveI	ACCTGC	2 cut(s)	619, 1090
Cac8I	GCNNGC	4 cut(s)	1233, 1408, 1552, 1573
CaiI	CAGNNNCTG	1 cut(s)	538
CciI	TCATGA	1 cut(s)	1048
CfoI	GCGC	1 cut(s)	1011
Cfr13I	GGNCC	4 cut(s)	74, 553, 753, 860
CseI	GACGC	2 cut(s)	93, 572
Csp6I	GTAC	1 cut(s)	1392
CspCI	CAANNNNNGTGG	2 cut(s)	1456, 1491
CviAII	CATG	7 cut(s)	517, 662, 742, 1049, 1343, 1351, 1411
CviQI	GTAC	1 cut(s)	1392
DdeI	CTNAG	9 cut(s)	233, 239, 539, 624, 1074, 1446, 1504, 1514, 1704
DpnI	GATC	4 cut(s)	849, 1179, 1202, 1609
DpnII	GATC	4 cut(s)	847, 1177, 1200, 1607
DraI	TTTAAA	1 cut(s)	1158
DrdI	GACNNNNNNGTC	1 cut(s)	1813
DseDI	GACNNNNNNGTC	1 cut(s)	1813
EaeI	YGGCCR	1 cut(s)	916
Eam1104I	CTCTTC	2 cut(s)	56, 1721
EarI	CTCTTC	2 cut(s)	56, 1721
Eco130I	CCWWGG	2 cut(s)	919, 991
Eco147I	AGGCCT	1 cut(s)	139
Eco31I	GGTCTC	2 cut(s)	700, 946
Eco47I	GGWCC	3 cut(s)	74, 753, 860
Eco88I	CYCGRG	1 cut(s)	588
EcoO109I	RGGNCCY	1 cut(s)	753
EcoRI	GAATTC	1 cut(s)	1369
EcoRII	CCWGG	4 cut(s)	116, 578, 711, 757
EcoT14I	CCWWGG	2 cut(s)	919, 991
ErhI	CCWWGG	2 cut(s)	919, 991
Esp3I	CGTCTC	1 cut(s)	71
FaeI	CATG	7 cut(s)	520, 665, 745, 1052, 1346, 1354, 1414
FalI	AAGNNNNNCTT	2 cut(s)	180, 212
FaqI	GGGAC	5 cut(s)	153, 570, 766, 1335, 1815
FatI	CATG	7 cut(s)	516, 661, 741, 1048, 1342, 1350, 1410
FauI	CCCGC	2 cut(s)	85, 1559
FblI	GTMKAC	3 cut(s)	677, 1338, 1521
Fnu4HI	GCNGC	5 cut(s)	12, 536, 1097, 1314, 1755
FokI	GGATG	5 cut(s)	560, 797, 839, 1346, 1550
Fsp4HI	GCNGC	5 cut(s)	12, 536, 1097, 1314, 1755
FspBI	CTAG	4 cut(s)	612, 1164, 1310, 1325
FspI	TGCGCA	1 cut(s)	1010
GlaI	GCGC	1 cut(s)	1010
GluI	GCNGC	5 cut(s)	12, 536, 1097, 1314, 1755
GsaI	CCCAGC	1 cut(s)	452
HaeIII	GGCC	4 cut(s)	139, 554, 918, 1732
HapII	CCGG	2 cut(s)	72, 1395
HgaI	GACGC	2 cut(s)	93, 572
HhaI	GCGC	1 cut(s)	1011
Hin1I	GRCGYC	1 cut(s)	583
Hin1II	CATG	7 cut(s)	520, 665, 745, 1052, 1346, 1354, 1414
Hin6I	GCGC	1 cut(s)	1009
HinP1I	GCGC	1 cut(s)	1009
HindIII	AAGCTT	1 cut(s)	1061
HinfI	GANTC	9 cut(s)	444, 669, 719, 766, 895, 924, 1508, 1593, 1829
HpaII	CCGG	2 cut(s)	72, 1395
HphI	GGTGA	2 cut(s)	815, 1235
Hpy166II	GTNNAC	4 cut(s)	678, 1302, 1339, 1522
Hpy188I	TCNGA	6 cut(s)	625, 975, 1075, 1200, 1605, 1738
Hpy188III	TCNNGA	9 cut(s)	224, 250, 863, 1049, 1106, 1204, 1267, 1531, 1626
Hpy8I	GTNNAC	4 cut(s)	678, 1302, 1339, 1522
HpyAV	CCTTC	7 cut(s)	32, 455, 537, 756, 1394, 1695, 1740
HpyCH4III	ACNGT	5 cut(s)	163, 472, 934, 1223, 1526
HpyCH4IV	ACGT	2 cut(s)	64, 1292
HpyF10VI	GCNNNNNNNGC	2 cut(s)	217, 625
HpyF3I	CTNAG	9 cut(s)	233, 239, 539, 624, 1074, 1446, 1504, 1514, 1704
HpySE526I	ACGT	2 cut(s)	64, 1292
Hsp92I	GRCGYC	1 cut(s)	583
Hsp92II	CATG	7 cut(s)	520, 665, 745, 1052, 1346, 1354, 1414
HspAI	GCGC	1 cut(s)	1009
KflI	GGGWCCC	1 cut(s)	753
Kzo9I	GATC	4 cut(s)	847, 1177, 1200, 1607
LmnI	GCTCC	1 cut(s)	8
Lsp1109I	GCAGC	5 cut(s)	23, 522, 1083, 1300, 1741
LweI	GCATC	2 cut(s)	1046, 1667
MaeI	CTAG	4 cut(s)	612, 1164, 1310, 1325
MaeII	ACGT	2 cut(s)	64, 1292
MaeIII	GTNAC	3 cut(s)	803, 1017, 1429
MalI	GATC	4 cut(s)	849, 1179, 1202, 1609
MboI	GATC	4 cut(s)	847, 1177, 1200, 1607
MfeI	CAATTG	1 cut(s)	735
MflI	RGATCY	2 cut(s)	1200, 1607
MhlI	GDGCHC	3 cut(s)	284, 1304, 1552
MlsI	TGGCCA	1 cut(s)	918
MluCI	AATT	7 cut(s)	365, 429, 735, 1159, 1213, 1369, 1583
MluNI	TGGCCA	1 cut(s)	918
MlyI	GAGTC	1 cut(s)	1602
MmeI	TCCRAC	4 cut(s)	369, 438, 804, 1713
Mox20I	TGGCCA	1 cut(s)	918
MscI	TGGCCA	1 cut(s)	918
MseI	TTAA	4 cut(s)	125, 890, 1157, 1761
Msp20I	TGGCCA	1 cut(s)	918
MspA1I	CMGCKG	2 cut(s)	535, 1754
MspI	CCGG	2 cut(s)	72, 1395
MspR9I	CCNGG	5 cut(s)	72, 118, 580, 713, 759
MunI	CAATTG	1 cut(s)	735
Mva1269I	GAATGC	2 cut(s)	211, 411
MvaI	CCWGG	4 cut(s)	118, 580, 713, 759
MwoI	GCNNNNNNNGC	2 cut(s)	217, 625
NciI	CCSGG	1 cut(s)	72
NdeII	GATC	4 cut(s)	847, 1177, 1200, 1607
NlaIII	CATG	7 cut(s)	520, 665, 745, 1052, 1346, 1354, 1414
NlaIV	GGNNCC	6 cut(s)	76, 245, 754, 755, 1230, 1417
NmeAIII	GCCGAG	1 cut(s)	1193
NmuCI	GTSAC	3 cut(s)	803, 1017, 1429
NsbI	TGCGCA	1 cut(s)	1010
NspI	RCATGY	1 cut(s)	520
NspV	TTCGAA	1 cut(s)	905
PagI	TCATGA	1 cut(s)	1048
PaqCI	CACCTGC	1 cut(s)	619
PceI	AGGCCT	1 cut(s)	139
PciI	ACATGT	1 cut(s)	516
PctI	GAATGC	2 cut(s)	211, 411
PfeI	GAWTC	8 cut(s)	444, 669, 719, 766, 895, 924, 1508, 1829
PflFI	GACNNNGTC	1 cut(s)	1526
PkrI	GCNGC	5 cut(s)	13, 537, 1098, 1315, 1756
PleI	GAGTC	1 cut(s)	1601
PpsI	GAGTC	1 cut(s)	1601
PpuMI	RGGWCCY	1 cut(s)	753
PscI	ACATGT	1 cut(s)	516
PshAI	GACNNNNGTC	1 cut(s)	473
PsiI	TTATAA	1 cut(s)	569
Psp1406I	AACGTT	1 cut(s)	1292
Psp5II	RGGWCCY	1 cut(s)	753
Psp6I	CCWGG	4 cut(s)	116, 578, 711, 757
PspFI	CCCAGC	1 cut(s)	448
PspGI	CCWGG	4 cut(s)	116, 578, 711, 757
PspN4I	GGNNCC	6 cut(s)	76, 245, 754, 755, 1230, 1417
PspPI	GGNCC	4 cut(s)	74, 553, 753, 860
PspPPI	RGGWCCY	1 cut(s)	753
PstI	CTGCAG	2 cut(s)	1101, 1759
PstNI	CAGNNNCTG	1 cut(s)	538
PsuI	RGATCY	2 cut(s)	1200, 1607
PsyI	GACNNNGTC	1 cut(s)	1526
PvuII	CAGCTG	2 cut(s)	535, 1754
RsaI	GTAC	1 cut(s)	1393
RsaNI	GTAC	1 cut(s)	1392
SaqAI	TTAA	4 cut(s)	125, 890, 1157, 1761
SatI	GCNGC	5 cut(s)	12, 536, 1097, 1314, 1755
Sau3AI	GATC	4 cut(s)	847, 1177, 1200, 1607
Sau96I	GGNCC	4 cut(s)	74, 553, 753, 860
SchI	GAGTC	1 cut(s)	1602
ScrFI	CCNGG	5 cut(s)	72, 118, 580, 713, 759
SduI	GDGCHC	3 cut(s)	284, 1304, 1552
SfaNI	GCATC	2 cut(s)	1046, 1667
SfcI	CTRYAG	2 cut(s)	1097, 1755
SfuI	TTCGAA	1 cut(s)	905
SinI	GGWCC	3 cut(s)	74, 753, 860
SmlI	CTYRAG	3 cut(s)	224, 248, 1106
SmoI	CTYRAG	3 cut(s)	224, 248, 1106
Sse9I	AATT	7 cut(s)	365, 429, 735, 1159, 1213, 1369, 1583
SseBI	AGGCCT	1 cut(s)	139
SsiI	CCGC	3 cut(s)	78, 690, 1552
SspMI	CTAG	4 cut(s)	612, 1164, 1310, 1325
StuI	AGGCCT	1 cut(s)	139
StyD4I	CCNGG	5 cut(s)	70, 116, 578, 711, 757
StyI	CCWWGG	2 cut(s)	919, 991
TaaI	ACNGT	5 cut(s)	163, 472, 934, 1223, 1526
TaiI	ACGT	2 cut(s)	67, 1295
TaqI	TCGA	2 cut(s)	905, 1205
TasI	AATT	7 cut(s)	365, 429, 735, 1159, 1213, 1369, 1583
TfiI	GAWTC	8 cut(s)	444, 669, 719, 766, 895, 924, 1508, 1829
Tru1I	TTAA	4 cut(s)	125, 890, 1157, 1761
Tru9I	TTAA	4 cut(s)	125, 890, 1157, 1761
TscAI	CASTG	7 cut(s)	154, 160, 440, 459, 1026, 1531, 1842
TseFI	GTSAC	3 cut(s)	803, 1017, 1429
TseI	GCWGC	5 cut(s)	11, 535, 1096, 1313, 1754
Tsp45I	GTSAC	3 cut(s)	803, 1017, 1429
TspDTI	ATGAA	5 cut(s)	587, 640, 1045, 1206, 1376
TspGWI	ACGGA	3 cut(s)	205, 207, 1819
TspRI	CASTG	7 cut(s)	154, 160, 440, 459, 1026, 1531, 1842
Tth111I	GACNNNGTC	1 cut(s)	1526
VneI	GTGCAC	1 cut(s)	1300
VpaK11BI	GGWCC	3 cut(s)	74, 753, 860
XapI	RAATTY	4 cut(s)	429, 1159, 1369, 1583
XceI	RCATGY	1 cut(s)	520
XmiI	GTMKAC	3 cut(s)	677, 1338, 1521
XspI	CTAG	4 cut(s)	612, 1164, 1310, 1325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.