Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006583

Physical Location & Seq

Genomic Coordinates Forward (+)

7720455 .. 7731708

Gene Structure 11254 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1044900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGGTAGTCTCAACCAGCAGCTCATTTTGGTGGATAAGTTTGAGCAGCATTTCTGTTATGGAGCACCCATTTGGTTTTGGAGGATTTTCGATTCCACTTCTACTCTCCGAGCTTAGCAATTCCTTGGTTACTGATCGTTTGCCCATAGGCATCGCTAGTGTTCGTATATGCTTATCTACAATTACGGAAAGCATTCAATATTTTCTCTTCTGTTTTTGTAATCTGTACTTTGTAGTAGAGGTGTCGCTATTTACAAAGATTAAATTTCTTTTGATGAGCATATACGAATTGTATTGTCAAGGGCGATCGTTTGAGGATCTTCCTTATGGTCGAGACAATTTTCTCAAAGTTTTTGAAAGGAAAATGGAAGAAAAGCGTAAACGCCTAAAAGATAGTGGAAATGAGCTTGCATTCTCTTGGGCTCTGTGTGCTGTCTGCCTAGTTGGCCATGCATCTCATTTCTTTGGGGCTAAGGCTCCATGGATCCATGCGCTCCATTCGACAGGATTCCATTTGTCCTTATGTCTATTTACATTGCTTGGTCCTGGGCGTCGACTTATCTTTGATGGTTTGAGGAGTCTTGTTAAAGGGGCTCCAAACATGAATACTTTGGTTGGTTTAGGGGCCTTATCGTCATTTACTGTCAGTTCATTAGCTGCCTTTATACCAAAACTGGGCTGGAAGACTTTCTTCGAGGAACCAATTATGTTGATAGCATTTGTCTTGTTAGGGAGGAATCTTGAACAAAGAGCTAAAATCAAAGCAAGCAGTGATATGACTGAACTTCTTAGTATTGTACCGTCAAAGGCACGTCTTTTGGTCAATAACGGTGAACAAGAGTTGGAATCAGTAGTTGAAGTTTCTACTAACAGCCTCTCTGTTGGAGATAAAATTGTTGTACTACCTGGAGACCGTGTTCCAGTAGATGGAATTGTTAAAGCTGGTAGGAGTATCATTGATGAGTCAAGTTTTACAGGAGAGCCGTTGCCTGTAACCAAACTACCTGGGAGTCAAGTTGCCGCCGGAAGCATAAATCTCAATGGCACTCTTACAGTTGAAGTACAGAGACCAGGTGGTGAGACTGCTATGGCAGACATTGTTCGCTTGGTAGAAGAAGCACAGAGTAGGGAAGCTCCTGTACAGCGCTTGGCTGACAAGGTGTCTGGACATTTTACATATGGAGTAATGACACTCTCTGCTGCTACATTTCTATTTTGGAGCTTGATTGGTGGTCATATTTTACCTGCTGCTTTTCACGGAGGAAATTCAGTTTCGTTAGCATTGCAACTCTCTTGCAGTGTACTGGTTGTTGCTTGTCCATGTGCTCTTGGGCTAGCCACACCTACAGCTGTGCTGGTTGGGACTTCATTGGGAGCTAAAAGAGGACTACTTTTGCGTGGCGGTAGTATTTTAGAGAAGTTTTCAATGGTGAACACTATAGTGTTTGATAAAACAGGGACTCTGACAGTGGGCAAACCTGTTGTGACTAAGATTGTGACTCCTGAACGTTCAAAAGTTACTGACTTAAAAGAAAAGATAAACCATACTTGGTCAGAAGTTGATGTTCTGAAGTTTGCTGCTGGAGTAGAATCGAATACAGTTCATCCTGTTGCAAAAGCTATTGTGGAAGCTAGTCAGGCTCTTAATTGTCAGAATATGAAGGTTGCGGATGGAACATTCTTGGAAGAACCTGGCTCTGGTGCTGTAGCAACTGTTGAGAACAAAAAGGTTTCTGTTGGGACTTTGGAATGGGTTCAAAGGCATGGAGTTACTAAGAATCCATTTGACGAAGTAGAAGCCCATACGAGTCAATCTATTGTATATGTAGGCATTGATAGTACTCTTGCCGGTCTCATTTATTTGGAGGATCAGATAAGGGATGATGCTGGACAAGTTGTTAAATCTTTATCTAAGCAAGGGATCGATGTATACATGCTATCTGGGGACAAAAGAAATAATGCGGAGTACGTCGCGTCTGCTGTTGGTATTCCGAAAGAGAAGGTGATATCTGGTGTTAAACCGACGGAAAAGAAGAAGTTCATAATGGAGCTTCAGAAGGATCAGAACATTGTAGCAATGGTTGGTGATGGAATTAATGATGCTGCAGCATTGGCTTCATCGCATGTTGGAATTGCCATGGGTGGTGGTGTTGGAGCTGCTAGTGAGGTGTCTTCTATTGTGCTATTGGGCAACAGACTTTCACAGTTACTTGATGCATTGGAGCTGAGTAGACTAACTATGAAGACTGTGAAGCAAAATCTGTGGTGGGCTTTTGCATACAATATTGTAGGACTTCCAATTGCTGCTGGAGTGTTGCTTCCAGTAACTGGGACCATTCTGACGCCGTCAATTGCTGGAGGCCTCATGGGCTTGAGTTCTGTAGGGGTTATGGCAAATTCATTGTTTCTGAGATACAAGTTCTCTAAACAGGAACACAAATATAGTGGATCTGCTCGCTCCAAAACTAACGAGGAATCCGATCTTCTCATCGACGTAAGTGCAGAAGAAGAGCATCCTCGTTCTGATGGTAAATGGAAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008152 GO:0008324 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009767 GO:0009941 GO:0009987 GO:0015075 GO:0015318 GO:0015979 GO:0016530 GO:0016531 GO:0019684 GO:0022857 GO:0022890 GO:0022900 GO:0030001 GO:0031967 GO:0031975 GO:0034220 GO:0035434 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046873 GO:0046914 GO:0046915 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055070 GO:0055076 GO:0055080 GO:0055085 GO:0055114 GO:0065007 GO:0065008 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0140104

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

848

Amino Acids

91.18

Weight (kDa)

6.98

Isoelectric Point (pI)

30.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	267 - 371	4.4e-23	P-type ATPase actuator domain
Hydrolase	PF00702	478 - 706	2.6e-29	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33520 AT4G33520
fragaria_vesca	FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930
malus_domestica	MD05G1044900.v1.1
prunus_persica	Prupe.8G060400_v2.0.a1
pyrus_communis	pycom05g03740
rosa_chinensis	RchiOBHm_Chr6g0271821
rosa_laevigata	RLG00000013712
rosa_multiflora	Rmu_sc0002396.1_g000018
rosa_roxburghii	Rroxscaffold_173G00435020 Rroxscaffold_7G00196710
rosa_rugosa	Rorug06G0063300 Rorug06G0063400
rosa_samantha	Rh6AG181500 Rh6BG186000 Rh6CG182200 Rh6DG173300
rosa_wichuraiana	Rw6G015660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1252
AccB7I	CCANNNNNTGG	2 cut(s)	926, 2327
AccI	GTMKAC	3 cut(s)	553, 1929, 2230
AccII	CGCG	1 cut(s)	1973
AciI	CCGC	4 cut(s)	1020, 1401, 1667, 1961
AclI	AACGTT	1 cut(s)	1507
AclWI	GGATC	7 cut(s)	324, 478, 491, 1875, 1928, 2067, 2455
AcoI	YGGCCR	1 cut(s)	445
AcsI	RAATTY	3 cut(s)	263, 1264, 2395
AcuI	CTGAAG	2 cut(s)	1589, 2036
AcyI	GRCGYC	2 cut(s)	550, 2342
AfaI	GTAC	8 cut(s)	227, 798, 900, 1062, 1140, 1302, 1840, 1967
AfeI	AGCGCT	1 cut(s)	1145
AfiI	CCNNNNNNNGG	4 cut(s)	674, 926, 1697, 2327
AgsI	TTSAA	8 cut(s)	197, 356, 743, 857, 1058, 1425, 1512, 1757
AhdI	GACNNNNNGTC	1 cut(s)	1159
AjiI	CACGTC	1 cut(s)	812
AjnI	CCWGG	5 cut(s)	544, 904, 1003, 1069, 1690
AleI	CACNNNNGTG	1 cut(s)	1439
AloI	GAACNNNNNNTCC	2 cut(s)	900, 932
Alw21I	GWGCWC	2 cut(s)	66, 1327
Alw26I	GTCTC	6 cut(s)	13, 327, 903, 1060, 1073, 1856
AlwI	GGATC	7 cut(s)	324, 478, 491, 1875, 1928, 2067, 2455
AlwNI	CAGNNNCTG	1 cut(s)	2327
Aor51HI	AGCGCT	1 cut(s)	1145
AoxI	GGCC	3 cut(s)	445, 624, 2359
ApoI	RAATTY	3 cut(s)	263, 1264, 2395
AseI	ATTAAT	1 cut(s)	2094
Asp700I	GAANNNNTTC	4 cut(s)	192, 686, 1752, 2036
AspLEI	GCGC	2 cut(s)	493, 1146
AspS9I	GGNCC	3 cut(s)	542, 624, 2331
AsuHPI	GGTGA	5 cut(s)	842, 1088, 1441, 2014, 2096
AsuNHI	GCTAGC	1 cut(s)	1333
AvaII	GGWCC	2 cut(s)	542, 2331
BalI	TGGCCA	1 cut(s)	447
BamHI	GGATCC	1 cut(s)	483
BanII	GRGCYC	2 cut(s)	424, 595
BarI	GAAGNNNNNNTAC	2 cut(s)	1122, 1154
BbsI	GAAGAC	3 cut(s)	689, 2163, 2249
Bbv12I	GWGCWC	2 cut(s)	66, 1327
BccI	CCATC	5 cut(s)	560, 920, 1664, 2081, 2519
BceAI	ACGGC	2 cut(s)	967, 2329
BcgI	CGANNNNNNTGC	2 cut(s)	2480, 2514
BciT130I	CCWGG	5 cut(s)	546, 906, 1005, 1071, 1692
BcoDI	GTCTC	6 cut(s)	13, 327, 903, 1060, 1073, 1856
BfaI	CTAG	5 cut(s)	156, 440, 1334, 1632, 2160
BfmI	CTRYAG	5 cut(s)	1344, 1437, 1704, 2103, 2379
BfoI	RGCGCY	1 cut(s)	1147
BfuAI	ACCTGC	1 cut(s)	1252
BglI	GCCNNNNNGGC	2 cut(s)	444, 2367
BlpI	GCTNAGC	1 cut(s)	113
BmcAI	AGTACT	1 cut(s)	1840
Bme1390I	CCNGG	5 cut(s)	546, 906, 1005, 1071, 1692
Bme18I	GGWCC	2 cut(s)	542, 2331
BmeRI	GACNNNNNGTC	1 cut(s)	1159
BmgBI	CACGTC	1 cut(s)	812
BmgT120I	GGNCC	3 cut(s)	542, 624, 2331
BmiI	GGNNCC	6 cut(s)	477, 485, 594, 625, 699, 2332
BmrFI	CCNGG	5 cut(s)	546, 906, 1005, 1071, 1692
BmrI	ACTGGG	2 cut(s)	683, 2337
BmsI	GCATC	6 cut(s)	159, 461, 1873, 2089, 2203, 2521
BmtI	GCTAGC	1 cut(s)	1337
BmuI	ACTGGG	2 cut(s)	683, 2337
BpiI	GAAGAC	3 cut(s)	689, 2163, 2249
BpmI	CTGGAG	4 cut(s)	927, 1602, 2328, 2376
Bpu10I	CCTNAGC	1 cut(s)	471
Bpu1102I	GCTNAGC	1 cut(s)	113
BpuEI	CTTGAG	1 cut(s)	2392
Bsa29I	ATCGAT	1 cut(s)	1923
BsaHI	GRCGYC	2 cut(s)	550, 2342
BsaI	GGTCTC	3 cut(s)	903, 1060, 1856
BsaJI	CCNNGG	5 cut(s)	123, 479, 545, 1004, 2136
BsaXI	ACNNNNNCTCC	2 cut(s)	900, 930
Bsc4I	CCNNNNNNNGG	4 cut(s)	674, 926, 1697, 2327
Bse118I	RCCGGY	1 cut(s)	1847
Bse1I	ACTGG	5 cut(s)	678, 920, 1308, 2321, 2332
Bse3DI	GCAATG	3 cut(s)	533, 1280, 2082
BseBI	CCWGG	5 cut(s)	546, 906, 1005, 1071, 1692
BseCI	ATCGAT	1 cut(s)	1923
BseDI	CCNNGG	5 cut(s)	123, 479, 545, 1004, 2136
BseGI	GGATG	4 cut(s)	1603, 1675, 1885, 2512
BseLI	CCNNNNNNNGG	4 cut(s)	674, 926, 1697, 2327
BseMI	GCAATG	3 cut(s)	533, 1280, 2082
BseMII	CTCAG	2 cut(s)	2216, 2399
BseNI	ACTGG	5 cut(s)	678, 920, 1308, 2321, 2332
BseRI	GAGGAG	1 cut(s)	589
BsgI	GTGCAG	1 cut(s)	2520
Bsh1236I	CGCG	1 cut(s)	1973
Bsh1285I	CGRYCG	1 cut(s)	308
BshFI	GGCC	3 cut(s)	447, 626, 2361
BshVI	ATCGAT	1 cut(s)	1923
BsiEI	CGRYCG	1 cut(s)	308
BsiHKAI	GWGCWC	2 cut(s)	66, 1327
BsiSI	CCGG	2 cut(s)	1023, 1848
BslFI	GGGAC	5 cut(s)	1375, 1471, 1753, 1958, 2344
BslI	CCNNNNNNNGG	4 cut(s)	674, 926, 1697, 2327
BsmAI	GTCTC	6 cut(s)	13, 327, 903, 1060, 1073, 1856
BsmFI	GGGAC	5 cut(s)	1375, 1471, 1753, 1958, 2344
BsmI	GAATGC	2 cut(s)	192, 410
BsnI	GGCC	3 cut(s)	447, 626, 2361
Bso31I	GGTCTC	3 cut(s)	903, 1060, 1856
Bsp1286I	GDGCHC	4 cut(s)	66, 424, 595, 1327
Bsp1407I	TGTACA	1 cut(s)	1138
Bsp143I	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
Bsp1720I	GCTNAGC	1 cut(s)	113
Bsp19I	CCATGG	2 cut(s)	479, 2136
BspACI	CCGC	4 cut(s)	1020, 1401, 1667, 1961
BspANI	GGCC	3 cut(s)	447, 626, 2361
BspCNI	CTCAG	2 cut(s)	2217, 2400
BspDI	ATCGAT	1 cut(s)	1923
BspFNI	CGCG	1 cut(s)	1973
BspLI	GGNNCC	6 cut(s)	477, 485, 594, 625, 699, 2332
BspMAI	CTGCAG	1 cut(s)	2107
BspMI	ACCTGC	1 cut(s)	1252
BspOI	GCTAGC	1 cut(s)	1337
BspPI	GGATC	7 cut(s)	324, 478, 491, 1875, 1928, 2067, 2455
BspQI	GCTCTTC	1 cut(s)	2502
BspTNI	GGTCTC	3 cut(s)	903, 1060, 1856
BsrDI	GCAATG	3 cut(s)	533, 1280, 2082
BsrFI	RCCGGY	1 cut(s)	1847
BsrGI	TGTACA	1 cut(s)	1138
BsrI	ACTGG	5 cut(s)	678, 920, 1308, 2321, 2332
BssAI	RCCGGY	1 cut(s)	1847
BssECI	CCNNGG	5 cut(s)	123, 479, 545, 1004, 2136
BssMI	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
BssNAI	GTATAC	1 cut(s)	1930
BssNI	GRCGYC	2 cut(s)	550, 2342
BssT1I	CCWWGG	3 cut(s)	123, 479, 2136
Bst1107I	GTATAC	1 cut(s)	1930
Bst2UI	CCWGG	5 cut(s)	546, 906, 1005, 1071, 1692
Bst6I	CTCTTC	2 cut(s)	212, 2502
BstACI	GRCGYC	2 cut(s)	550, 2342
BstAUI	TGTACA	1 cut(s)	1138
BstC8I	GCNNGC	4 cut(s)	408, 766, 1335, 2455
BstDEI	CTNAG	8 cut(s)	113, 471, 788, 1488, 1773, 1911, 2225, 2408
BstDSI	CCRYGG	2 cut(s)	479, 2136
BstF5I	GGATG	4 cut(s)	1603, 1675, 1885, 2512
BstFNI	CGCG	1 cut(s)	1973
BstH2I	RGCGCY	1 cut(s)	1147
BstHHI	GCGC	2 cut(s)	493, 1146
BstKTI	GATC	9 cut(s)	136, 308, 319, 486, 1870, 1923, 2062, 2450, 2482
BstMAI	GTCTC	6 cut(s)	13, 327, 903, 1060, 1073, 1856
BstMBI	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
BstMCI	CGRYCG	1 cut(s)	308
BstMWI	GCNNNNNNNGC	5 cut(s)	428, 444, 1637, 2111, 2367
BstNI	CCWGG	5 cut(s)	546, 906, 1005, 1071, 1692
BstNSI	RCATGY	2 cut(s)	1936, 2126
BstSCI	CCNGG	5 cut(s)	544, 904, 1003, 1069, 1690
BstSFI	CTRYAG	5 cut(s)	1344, 1437, 1704, 2103, 2379
BstUI	CGCG	1 cut(s)	1973
BstV2I	GAAGAC	3 cut(s)	689, 2163, 2249
BstX2I	RGATCY	3 cut(s)	316, 483, 2447
BstYI	RGATCY	3 cut(s)	316, 483, 2447
BstZ17I	GTATAC	1 cut(s)	1930
Bsu15I	ATCGAT	1 cut(s)	1923
BsuRI	GGCC	3 cut(s)	447, 626, 2361
BsuTUI	ATCGAT	1 cut(s)	1923
BtgI	CCRYGG	2 cut(s)	479, 2136
BtgZI	GCGATG	2 cut(s)	136, 2103
BtrI	CACGTC	1 cut(s)	812
BtsCI	GGATG	4 cut(s)	1603, 1675, 1885, 2512
BtsI	GCAGTG	2 cut(s)	775, 1303
BtsIMutI	CAGTG	3 cut(s)	775, 1303, 1473
BveI	ACCTGC	1 cut(s)	1252
Cac8I	GCNNGC	4 cut(s)	408, 766, 1335, 2455
CaiI	CAGNNNCTG	1 cut(s)	2327
CfoI	GCGC	2 cut(s)	493, 1146
Cfr10I	RCCGGY	1 cut(s)	1847
Cfr13I	GGNCC	3 cut(s)	542, 624, 2331
ClaI	ATCGAT	1 cut(s)	1923
CseI	GACGC	3 cut(s)	539, 1962, 2350
CsiI	ACCWGGT	1 cut(s)	1069
Csp6I	GTAC	8 cut(s)	226, 797, 899, 1061, 1139, 1301, 1839, 1966
CspCI	CAANNNNNGTGG	4 cut(s)	2243, 2278, 2425, 2460
CviQI	GTAC	8 cut(s)	226, 797, 899, 1061, 1139, 1301, 1839, 1966
DdeI	CTNAG	8 cut(s)	113, 471, 788, 1488, 1773, 1911, 2225, 2408
DpnI	GATC	9 cut(s)	135, 307, 318, 485, 1869, 1922, 2061, 2449, 2481
DpnII	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
DriI	GACNNNNNGTC	1 cut(s)	1159
EaeI	YGGCCR	1 cut(s)	445
Eam1104I	CTCTTC	2 cut(s)	212, 2502
Eam1105I	GACNNNNNGTC	1 cut(s)	1159
EarI	CTCTTC	2 cut(s)	212, 2502
Eco130I	CCWWGG	3 cut(s)	123, 479, 2136
Eco147I	AGGCCT	1 cut(s)	2361
Eco24I	GRGCYC	2 cut(s)	424, 595
Eco31I	GGTCTC	3 cut(s)	903, 1060, 1856
Eco32I	GATATC	1 cut(s)	2007
Eco47I	GGWCC	2 cut(s)	542, 2331
Eco47III	AGCGCT	1 cut(s)	1145
Eco57I	CTGAAG	2 cut(s)	1589, 2036
EcoO109I	RGGNCCY	1 cut(s)	624
EcoRII	CCWGG	5 cut(s)	544, 904, 1003, 1069, 1690
EcoRV	GATATC	1 cut(s)	2007
EcoT14I	CCWWGG	3 cut(s)	123, 479, 2136
EcoT22I	ATGCAT	2 cut(s)	454, 2218
EcoT38I	GRGCYC	2 cut(s)	424, 595
ErhI	CCWWGG	3 cut(s)	123, 479, 2136
FalI	AAGNNNNNCTT	2 cut(s)	674, 706
FaqI	GGGAC	5 cut(s)	1375, 1471, 1753, 1958, 2344
FauNDI	CATATG	1 cut(s)	1177
FblI	GTMKAC	3 cut(s)	553, 1929, 2230
FokI	GGATG	4 cut(s)	1590, 1682, 1892, 2499
FriOI	GRGCYC	2 cut(s)	424, 595
FspBI	CTAG	5 cut(s)	156, 440, 1334, 1632, 2160
GlaI	GCGC	2 cut(s)	492, 1145
GsuI	CTGGAG	4 cut(s)	927, 1602, 2328, 2376
HaeII	RGCGCY	1 cut(s)	1147
HaeIII	GGCC	3 cut(s)	447, 626, 2361
HapII	CCGG	2 cut(s)	1023, 1848
HgaI	GACGC	3 cut(s)	539, 1962, 2350
HhaI	GCGC	2 cut(s)	493, 1146
Hin1I	GRCGYC	2 cut(s)	550, 2342
Hin6I	GCGC	2 cut(s)	491, 1144
HinP1I	GCGC	2 cut(s)	491, 1144
HincII	GTYRAC	1 cut(s)	554
HindII	GTYRAC	1 cut(s)	554
HpaII	CCGG	2 cut(s)	1023, 1848
HphI	GGTGA	5 cut(s)	842, 1088, 1441, 2014, 2096
Hpy166II	GTNNAC	7 cut(s)	380, 554, 833, 1301, 1432, 1930, 2231
Hpy188III	TCNNGA	4 cut(s)	332, 740, 1164, 1502
Hpy8I	GTNNAC	7 cut(s)	380, 554, 833, 1301, 1432, 1930, 2231
Hpy99I	CGWCG	4 cut(s)	555, 1973, 2026, 2495
HpyAV	CCTTC	3 cut(s)	1654, 1993, 2050
HpyCH4IV	ACGT	4 cut(s)	811, 1507, 1968, 2493
HpyCH4V	TGCA	9 cut(s)	410, 452, 1285, 1296, 1613, 2105, 2216, 2276, 2501
HpyF10VI	GCNNNNNNNGC	5 cut(s)	428, 444, 1637, 2111, 2367
HpyF3I	CTNAG	8 cut(s)	113, 471, 788, 1488, 1773, 1911, 2225, 2408
HpySE526I	ACGT	4 cut(s)	811, 1507, 1968, 2493
Hsp92I	GRCGYC	2 cut(s)	550, 2342
HspAI	GCGC	2 cut(s)	491, 1144
Kzo9I	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
LguI	GCTCTTC	1 cut(s)	2502
LweI	GCATC	6 cut(s)	159, 461, 1873, 2089, 2203, 2521
MabI	ACCWGGT	1 cut(s)	1069
MaeI	CTAG	5 cut(s)	156, 440, 1334, 1632, 2160
MaeII	ACGT	4 cut(s)	811, 1507, 1968, 2493
MaeIII	GTNAC	8 cut(s)	127, 991, 1483, 1495, 1516, 1768, 2205, 2323
MalI	GATC	9 cut(s)	135, 307, 318, 485, 1869, 1922, 2061, 2449, 2481
MboI	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
MfeI	CAATTG	2 cut(s)	2298, 2349
MflI	RGATCY	3 cut(s)	316, 483, 2447
MhlI	GDGCHC	4 cut(s)	66, 424, 595, 1327
MlsI	TGGCCA	1 cut(s)	447
MluNI	TGGCCA	1 cut(s)	447
MlyI	GAGTC	6 cut(s)	586, 971, 1018, 1453, 1492, 1816
MmeI	TCCRAC	4 cut(s)	822, 862, 2107, 2131
Mox20I	TGGCCA	1 cut(s)	447
Mph1103I	ATGCAT	2 cut(s)	454, 2218
MroXI	GAANNNNTTC	4 cut(s)	192, 686, 1752, 2036
MscI	TGGCCA	1 cut(s)	447
MseI	TTAA	8 cut(s)	261, 585, 936, 1526, 1644, 1899, 2016, 2094
MslI	CAYNNNNRTG	2 cut(s)	28, 1439
Msp20I	TGGCCA	1 cut(s)	447
MspA1I	CMGCKG	1 cut(s)	1349
MspI	CCGG	2 cut(s)	1023, 1848
MspR9I	CCNGG	5 cut(s)	546, 906, 1005, 1071, 1692
MunI	CAATTG	2 cut(s)	2298, 2349
Mva1269I	GAATGC	2 cut(s)	192, 410
MvaI	CCWGG	5 cut(s)	546, 906, 1005, 1071, 1692
MvnI	CGCG	1 cut(s)	1973
MwoI	GCNNNNNNNGC	5 cut(s)	428, 444, 1637, 2111, 2367
NcoI	CCATGG	2 cut(s)	479, 2136
NdeI	CATATG	1 cut(s)	1177
NdeII	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
NheI	GCTAGC	1 cut(s)	1333
NlaIV	GGNNCC	6 cut(s)	477, 485, 594, 625, 699, 2332
NmuCI	GTSAC	2 cut(s)	1483, 1495
NsiI	ATGCAT	2 cut(s)	454, 2218
NspI	RCATGY	2 cut(s)	1936, 2126
OliI	CACNNNNGTG	1 cut(s)	1439
PceI	AGGCCT	1 cut(s)	2361
PciSI	GCTCTTC	1 cut(s)	2502
PcsI	WCGNNNNNNNCGW	1 cut(s)	2475
PctI	GAATGC	2 cut(s)	192, 410
PdmI	GAANNNNTTC	4 cut(s)	192, 686, 1752, 2036
PfeI	GAWTC	7 cut(s)	91, 507, 736, 845, 1589, 1777, 2474
PflFI	GACNNNGTC	1 cut(s)	2344
PflMI	CCANNNNNTGG	2 cut(s)	926, 2327
Ple19I	CGATCG	1 cut(s)	308
PleI	GAGTC	6 cut(s)	585, 970, 1017, 1453, 1492, 1815
PpsI	GAGTC	6 cut(s)	585, 970, 1017, 1453, 1492, 1815
PshBI	ATTAAT	1 cut(s)	2094
Psp1406I	AACGTT	1 cut(s)	1507
Psp6I	CCWGG	5 cut(s)	544, 904, 1003, 1069, 1690
PspGI	CCWGG	5 cut(s)	544, 904, 1003, 1069, 1690
PspN4I	GGNNCC	6 cut(s)	477, 485, 594, 625, 699, 2332
PspPI	GGNCC	3 cut(s)	542, 624, 2331
PstI	CTGCAG	1 cut(s)	2107
PstNI	CAGNNNCTG	1 cut(s)	2327
PsuI	RGATCY	3 cut(s)	316, 483, 2447
PsyI	GACNNNGTC	1 cut(s)	2344
PvuI	CGATCG	1 cut(s)	308
PvuII	CAGCTG	1 cut(s)	1349
RsaI	GTAC	8 cut(s)	227, 798, 900, 1062, 1140, 1302, 1840, 1967
RsaNI	GTAC	8 cut(s)	226, 797, 899, 1061, 1139, 1301, 1839, 1966
RseI	CAYNNNNRTG	2 cut(s)	28, 1439
SalI	GTCGAC	1 cut(s)	552
SapI	GCTCTTC	1 cut(s)	2502
SaqAI	TTAA	8 cut(s)	261, 585, 936, 1526, 1644, 1899, 2016, 2094
Sau3AI	GATC	9 cut(s)	133, 305, 316, 483, 1867, 1920, 2059, 2447, 2479
Sau96I	GGNCC	3 cut(s)	542, 624, 2331
ScaI	AGTACT	1 cut(s)	1840
SchI	GAGTC	6 cut(s)	586, 971, 1018, 1453, 1492, 1816
ScrFI	CCNGG	5 cut(s)	546, 906, 1005, 1071, 1692
SduI	GDGCHC	4 cut(s)	66, 424, 595, 1327
SexAI	ACCWGGT	1 cut(s)	1069
SfaNI	GCATC	6 cut(s)	159, 461, 1873, 2089, 2203, 2521
SfcI	CTRYAG	5 cut(s)	1344, 1437, 1704, 2103, 2379
SinI	GGWCC	2 cut(s)	542, 2331
SmiMI	CAYNNNNRTG	2 cut(s)	28, 1439
SmlI	CTYRAG	1 cut(s)	2371
SmoI	CTYRAG	1 cut(s)	2371
SseBI	AGGCCT	1 cut(s)	2361
SsiI	CCGC	4 cut(s)	1020, 1401, 1667, 1961
SspI	AATATT	2 cut(s)	200, 2284
SspMI	CTAG	5 cut(s)	156, 440, 1334, 1632, 2160
StuI	AGGCCT	1 cut(s)	2361
StyD4I	CCNGG	5 cut(s)	544, 904, 1003, 1069, 1690
StyI	CCWWGG	3 cut(s)	123, 479, 2136
TaiI	ACGT	4 cut(s)	814, 1510, 1971, 2496
TaqI	TCGA	8 cut(s)	89, 331, 500, 553, 693, 1592, 1923, 2490
TatI	WGTACW	6 cut(s)	225, 898, 1060, 1138, 1300, 1838
TauI	GCSGC	1 cut(s)	1022
TfiI	GAWTC	7 cut(s)	91, 507, 736, 845, 1589, 1777, 2474
Tru1I	TTAA	8 cut(s)	261, 585, 936, 1526, 1644, 1899, 2016, 2094
Tru9I	TTAA	8 cut(s)	261, 585, 936, 1526, 1644, 1899, 2016, 2094
TscAI	CASTG	3 cut(s)	775, 1303, 1473
TseFI	GTSAC	2 cut(s)	1483, 1495
Tsp45I	GTSAC	2 cut(s)	1483, 1495
TspDTI	ATGAA	9 cut(s)	617, 639, 1356, 1592, 1673, 2029, 2106, 2255, 2388
TspGWI	ACGGA	3 cut(s)	200, 1272, 2039
TspRI	CASTG	3 cut(s)	775, 1303, 1473
Tth111I	GACNNNGTC	1 cut(s)	2344
Van91I	CCANNNNNTGG	2 cut(s)	926, 2327
VpaK11BI	GGWCC	2 cut(s)	542, 2331
VspI	ATTAAT	1 cut(s)	2094
XapI	RAATTY	3 cut(s)	263, 1264, 2395
XceI	RCATGY	2 cut(s)	1936, 2126
XcmI	CCANNNNNNNNNTGG	1 cut(s)	675
XmiI	GTMKAC	3 cut(s)	553, 1929, 2230
XmnI	GAANNNNTTC	4 cut(s)	192, 686, 1752, 2036
XspI	CTAG	5 cut(s)	156, 440, 1334, 1632, 2160
ZrmI	AGTACT	1 cut(s)	1840
Zsp2I	ATGCAT	2 cut(s)	454, 2218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.