Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0006583

Physical Location & Seq

Genomic Coordinates Forward (+)

30270316 .. 30291440

Gene Structure 21125 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG173300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGAGTCAACACTGAACGCGAGCACATCGACCACATTGGCTCTGCTCACCGTTACGAAAGCCCTGAACCGCCGACTTTGCAAGATCTTTCCGAGTCATCAACCCCTCGCGAGTCGATTGATAGCTCGCAGGCTCACTTGCAACTACGGCGGCTTTCATAGGCAGAGGCCTCTCTCTTCATCGACGCTGAGAAGGAGCCTCTGCGTGGTTGATCCTCCGACACGGCGTCGTTTGGAGTGCGTTGCGAGCAACCATGCGTCATTTGCTTCCGGCGGTGGAAATGGTGGCTTCGGCGGTGAGAGTGGTGGCAGCGGCGGCGATGGTGGAGGTAGTTCGGACGGTGGCGATGCCAAGTCCAAGGTCGTTGCGTCTGGGGGCGATGACGTCTCGGCCGTCTCGACTGATGTCATACTGCTGGATGTTGGAGGAATGACATGCGGGGGATGTGCAGCAAGTGTGAAGCGAATTCTAGAAAGCCAACCACAGGTGTCATCTGCTAGTGTAAATCTTACAACTGAGGTGGCAATTGTCTGGCCTGTGTCTGAAGCCAAGCTCTCACCAAACTGGCAAAAGCAGTTGGGAGAGACACTTGCAAACCATTTGACTAATTGTGGTTTCAATTCTAACATTCGTGATTCAGGAAGAAACAGTTTTCTTAAGATGTTTGAAAGGAAAATTGAAGATAAGCGCAAACGCCTGAAAGAAAGTGGTCATGAGCTTGCTTTCTCTTGGGCTCTATGTGCTGTCTGCCTAGTTGGTCATCTTTCTCATTTCCTTGGAGCTAAGGCTTCATGGATCCATGCTTTTCATTCAACAGGATTCCATCTGTCTCTATCTTTATTCACATTGCTTGGTCCTGGGCGTCGGCTTATCCTTGATGGTTTGAAGAGTCTTGTTAAAGGGGCTCCAAACATGAACACTTTAGTTGGTCTTGGGGCTTTATCTTCATTTACAGTCAGTTCATTAGCTGCCTTTATACCAAAGCTGGGTTGGAAGACTTTCTTTGAGGAACCAATAATGTTGATAGCATTCGTGCTGCTGGGGAGGAATCTTGAACAAAGAGCTAAAATCAAAGCAAGCAGTGATATGACTGAGCTTCTTAGTATTTTACCAGCAAAAGCTCGTCTTCTGGTCAATGATGGTGTTAAAGAGTCGGAAACAATAGTTGAAGTTCCATCTAACAGTCTCTGTGTAGGAGATCAAGTTGTTGTATTACCTGGAGACCGTGTTCCAGTAGATGGAATTGTTATAGCTGGTAGAAGTACCATTGATGAGTCAAGTTTTACTGGAGAGCCATTGCCTGTGACCAAACTACCTGGGAGTCAAGTTGCAGCTGGAAGTATAAATCTCAATGGAAGTCTTACGATTGTAGTGCAGAGACCAGGTGGTGAGACTGCTATGGCAGACATTGTTCGTCTAGTAGAAGAAGCACAGAGTCGGGAGGCTCCAGTCCAGCGTCTGGCTGACAAGGTATCTGGGCATTTTACATATGGAGTAATGGGACTCTCTGCGGCTACATTTTTGTTCTGGAGTTTGATTGGTGGACGTATTTTACCTGCGGTTCTTCAAGGAGGAAATTCAGTTTCTTTAGCATTGCAACTCTCTTGCAGTGTTCTGGTTGTTGCTTGTCCATGTGCCCTTGGGCTGGCTACACCTACAGCTGTGCTGGTAGGAACTTCAATAGGAGCCACAAGAGGACTGCTTTTGCGTGGTGGAAATGTTTTAGAGAAGTTTTCGATGGTGAACACTGTAGTATTTGATAAAACAGGGACTCTGACAATGGGCAAGCCAGTTGTGACAAAAATTTTGACTCCCGAACATGCAGAAGTTACTGATGTAGAAGAAAAGTTGAACCATACTTGGTCAGACCTTGAGGTGCTCAAGTTTGCTGCTGGAGTAGAATCAAATACAATTCATCCTGTTGGGAAAGCTATTGTGGAAGCCGCTCGGGCTGTTAATTGTCAGCATATACAGGTTGCAGATGGAACATTTATAGAAGAACCTGGCTCTGGTGCTGTAGCTATTATTGAAGATAAAAAGGTTTCTGTTGGAACTTTGGACTGGGTCCAACGGCATGGAGTCAATGAGAATCCATTTGAAGAAGTTGAGGCCCATAAGAGTCAATCTGTTGTGTATGTTGCCATTGATAGTACTCTTGCTGGTCTTATTTATTTTGAGGATCAGATAAGAGATGATGCCGGACAAGTTGTTAAATCCTTATCTAGCCAAGGGATTTCTGTATACATGCTATCTGGGGACAAAACAGAAAACGCAGAGTACGTTGCATCTGTAGTTGGTATTCCAAAAGAGAAGGTCAGGGATTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008152 GO:0008324 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009767 GO:0009941 GO:0009987 GO:0015075 GO:0015318 GO:0015979 GO:0016530 GO:0016531 GO:0019684 GO:0022857 GO:0022890 GO:0022900 GO:0030001 GO:0031967 GO:0031975 GO:0034220 GO:0035434 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046873 GO:0046914 GO:0046915 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055070 GO:0055076 GO:0055080 GO:0055085 GO:0055114 GO:0065007 GO:0065008 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0140104

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

775

Amino Acids

82.0

Weight (kDa)

6.99

Isoelectric Point (pI)

38.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	139 - 183	5.5e-09	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	372 - 475	1.2e-25	P-type ATPase actuator domain
Hydrolase	PF00702	582 - 767	2.8e-18	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33520 AT4G33520
fragaria_vesca	FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930
malus_domestica	MD05G1044900.v1.1
prunus_persica	Prupe.8G060400_v2.0.a1
pyrus_communis	pycom05g03740
rosa_chinensis	RchiOBHm_Chr6g0271821
rosa_laevigata	RLG00000013712
rosa_multiflora	Rmu_sc0002396.1_g000018
rosa_roxburghii	Rroxscaffold_173G00435020 Rroxscaffold_7G00196710
rosa_rugosa	Rorug06G0063300 Rorug06G0063400
rosa_samantha	Rh6AG181500 Rh6BG186000 Rh6CG182200 Rh6DG173300
rosa_wichuraiana	Rw6G015660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	387
Acc36I	ACCTGC	1 cut(s)	1564
AccB7I	CCANNNNNTGG	2 cut(s)	1238, 1459
AccBSI	CCGCTC	1 cut(s)	1946
AccI	GTMKAC	1 cut(s)	2241
AccII	CGCG	2 cut(s)	20, 110
AclWI	GGATC	4 cut(s)	206, 790, 803, 2187
AcoI	YGGCCR	1 cut(s)	390
AcsI	RAATTY	3 cut(s)	465, 1576, 1803
AcuI	CTGAAG	1 cut(s)	564
AcyI	GRCGYC	3 cut(s)	226, 384, 862
AfaI	GTAC	3 cut(s)	1264, 2152, 2279
AfiI	CCNNNNNNNGG	7 cut(s)	205, 484, 986, 1238, 1459, 1645, 2009
AflII	CTTAAG	1 cut(s)	656
AhdI	GACNNNNNGTC	1 cut(s)	225
AjnI	CCWGG	5 cut(s)	856, 1216, 1315, 1381, 2002
AloI	GAACNNNNNNTCC	2 cut(s)	1212, 1244
Alw21I	GWGCWC	2 cut(s)	26, 1881
Alw26I	GTCTC	8 cut(s)	391, 400, 578, 834, 1190, 1215, 1372, 1385
AlwI	GGATC	4 cut(s)	206, 790, 803, 2187
AlwNI	CAGNNNCTG	2 cut(s)	1188, 1459
Ama87I	CYCGRG	1 cut(s)	1947
AoxI	GGCC	4 cut(s)	167, 390, 533, 2109
ApeKI	GCWGC	6 cut(s)	309, 449, 968, 1036, 1331, 1889
ApoI	RAATTY	3 cut(s)	465, 1576, 1803
ArsI	GACNNNNNNTTYG	2 cut(s)	1789, 1821
Asp700I	GAANNNNTTC	1 cut(s)	998
AspLEI	GCGC	1 cut(s)	690
AspS9I	GGNCC	3 cut(s)	854, 2065, 2110
AsuHPI	GGTGA	5 cut(s)	40, 308, 549, 1400, 1753
AvaI	CYCGRG	1 cut(s)	1947
AvaII	GGWCC	2 cut(s)	854, 2065
BaeGI	GKGCMC	1 cut(s)	1639
BamHI	GGATCC	1 cut(s)	795
BanII	GRGCYC	2 cut(s)	736, 907
BbsI	GAAGAC	2 cut(s)	1001, 1118
Bbv12I	GWGCWC	2 cut(s)	26, 1881
BbvI	GCAGC	6 cut(s)	321, 461, 955, 1023, 1343, 1876
BccI	CCATC	8 cut(s)	314, 831, 872, 1133, 1183, 1232, 1732, 1976
BceAI	ACGGC	4 cut(s)	163, 239, 377, 2087
BcgI	CGANNNNNNTGC	2 cut(s)	1354, 1388
BciT130I	CCWGG	5 cut(s)	858, 1218, 1317, 1383, 2004
BcoDI	GTCTC	8 cut(s)	391, 400, 578, 834, 1190, 1215, 1372, 1385
BfaI	CTAG	5 cut(s)	470, 498, 752, 1418, 2223
BfmI	CTRYAG	4 cut(s)	1656, 1749, 2016, 2289
BfrI	CTTAAG	1 cut(s)	656
BfuAI	ACCTGC	1 cut(s)	1564
BglI	GCCNNNNNGGC	1 cut(s)	1949
BglII	AGATCT	1 cut(s)	84
BmcAI	AGTACT	1 cut(s)	2152
Bme1390I	CCNGG	5 cut(s)	858, 1218, 1317, 1383, 2004
Bme18I	GGWCC	2 cut(s)	854, 2065
BmeRI	GACNNNNNGTC	1 cut(s)	225
BmeT110I	CYCGRG	1 cut(s)	1947
BmgT120I	GGNCC	3 cut(s)	854, 2065, 2110
BmiI	GGNNCC	7 cut(s)	197, 797, 906, 1011, 1446, 1687, 2066
BmrFI	CCNGG	5 cut(s)	858, 1218, 1317, 1383, 2004
BmrI	ACTGGG	1 cut(s)	2071
BmsI	GCATC	3 cut(s)	337, 2185, 2294
BmuI	ACTGGG	1 cut(s)	2071
BoxI	GACNNNNGTC	1 cut(s)	404
BpiI	GAAGAC	2 cut(s)	1001, 1118
BpmI	CTGGAG	5 cut(s)	1239, 1308, 1431, 1549, 1914
Bpu10I	CCTNAGC	1 cut(s)	783
BpuEI	CTTGAG	2 cut(s)	1865, 1892
BsaHI	GRCGYC	3 cut(s)	226, 384, 862
BsaI	GGTCTC	2 cut(s)	1215, 1372
BsaJI	CCNNGG	6 cut(s)	357, 775, 857, 1316, 1639, 2227
BsaXI	ACNNNNNCTCC	4 cut(s)	1188, 1212, 1218, 1242
Bsc4I	CCNNNNNNNGG	7 cut(s)	205, 484, 986, 1238, 1459, 1645, 2009
Bse1I	ACTGG	6 cut(s)	569, 1232, 1291, 1448, 1790, 2066
Bse3DI	GCAATG	3 cut(s)	845, 1295, 1592
BseBI	CCWGG	5 cut(s)	858, 1218, 1317, 1383, 2004
BseDI	CCNNGG	6 cut(s)	357, 775, 857, 1316, 1639, 2227
BseGI	GGATG	3 cut(s)	424, 449, 1915
BseLI	CCNNNNNNNGG	7 cut(s)	205, 484, 986, 1238, 1459, 1645, 2009
BseMI	GCAATG	3 cut(s)	845, 1295, 1592
BseMII	CTCAG	3 cut(s)	179, 507, 1083
BseNI	ACTGG	6 cut(s)	569, 1232, 1291, 1448, 1790, 2066
BseSI	GKGCMC	1 cut(s)	1639
BseX3I	CGGCCG	1 cut(s)	390
BseXI	GCAGC	6 cut(s)	321, 461, 955, 1023, 1343, 1876
BseYI	CCCAGC	2 cut(s)	985, 1039
BsgI	GTGCAG	2 cut(s)	468, 1394
Bsh1236I	CGCG	2 cut(s)	20, 110
Bsh1285I	CGRYCG	1 cut(s)	393
BshFI	GGCC	4 cut(s)	169, 392, 535, 2111
BsiEI	CGRYCG	1 cut(s)	393
BsiHKAI	GWGCWC	2 cut(s)	26, 1881
BsiHKCI	CYCGRG	1 cut(s)	1947
BsiSI	CCGG	2 cut(s)	270, 2199
BslFI	GGGAC	3 cut(s)	1515, 1783, 2270
BslI	CCNNNNNNNGG	7 cut(s)	205, 484, 986, 1238, 1459, 1645, 2009
BsmAI	GTCTC	8 cut(s)	391, 400, 578, 834, 1190, 1215, 1372, 1385
BsmBI	CGTCTC	2 cut(s)	391, 400
BsmFI	GGGAC	3 cut(s)	1515, 1783, 2270
BsmI	GAATGC	1 cut(s)	1028
BsnI	GGCC	4 cut(s)	169, 392, 535, 2111
Bso31I	GGTCTC	2 cut(s)	1215, 1372
BsoBI	CYCGRG	1 cut(s)	1947
Bsp1286I	GDGCHC	5 cut(s)	26, 736, 907, 1639, 1881
Bsp143I	GATC	5 cut(s)	84, 211, 795, 1198, 2179
Bsp68I	TCGCGA	1 cut(s)	110
BspANI	GGCC	4 cut(s)	169, 392, 535, 2111
BspCNI	CTCAG	3 cut(s)	180, 508, 1084
BspFNI	CGCG	2 cut(s)	20, 110
BspHI	TCATGA	1 cut(s)	712
BspLI	GGNNCC	7 cut(s)	197, 797, 906, 1011, 1446, 1687, 2066
BspMI	ACCTGC	1 cut(s)	1564
BspPI	GGATC	4 cut(s)	206, 790, 803, 2187
BspTI	CTTAAG	1 cut(s)	656
BspTNI	GGTCTC	2 cut(s)	1215, 1372
BsrBI	CCGCTC	1 cut(s)	1946
BsrDI	GCAATG	3 cut(s)	845, 1295, 1592
BsrI	ACTGG	6 cut(s)	569, 1232, 1291, 1448, 1790, 2066
BssECI	CCNNGG	6 cut(s)	357, 775, 857, 1316, 1639, 2227
BssMI	GATC	5 cut(s)	84, 211, 795, 1198, 2179
BssNAI	GTATAC	1 cut(s)	2242
BssNI	GRCGYC	3 cut(s)	226, 384, 862
BssT1I	CCWWGG	4 cut(s)	357, 775, 1639, 2227
Bst1107I	GTATAC	1 cut(s)	2242
Bst2UI	CCWGG	5 cut(s)	858, 1218, 1317, 1383, 2004
Bst4CI	ACNGT	7 cut(s)	52, 341, 650, 955, 1184, 1226, 1750
Bst6I	CTCTTC	2 cut(s)	181, 881
BstACI	GRCGYC	3 cut(s)	226, 384, 862
BstAFI	CTTAAG	1 cut(s)	656
BstC8I	GCNNGC	8 cut(s)	22, 127, 131, 247, 720, 1078, 1647, 1787
BstDEI	CTNAG	5 cut(s)	188, 516, 783, 1092, 1100
BstF5I	GGATG	3 cut(s)	424, 449, 1915
BstFNI	CGCG	2 cut(s)	20, 110
BstHHI	GCGC	1 cut(s)	690
BstKTI	GATC	5 cut(s)	87, 214, 798, 1201, 2182
BstMAI	GTCTC	8 cut(s)	391, 400, 578, 834, 1190, 1215, 1372, 1385
BstMBI	GATC	5 cut(s)	84, 211, 795, 1198, 2179
BstMCI	CGRYCG	1 cut(s)	393
BstMWI	GCNNNNNNNGC	7 cut(s)	78, 147, 246, 263, 315, 740, 1949
BstNI	CCWGG	5 cut(s)	858, 1218, 1317, 1383, 2004
BstNSI	RCATGY	3 cut(s)	438, 1823, 2248
BstPAI	GACNNNNGTC	1 cut(s)	404
BstSCI	CCNGG	5 cut(s)	856, 1216, 1315, 1381, 2002
BstSFI	CTRYAG	4 cut(s)	1656, 1749, 2016, 2289
BstSLI	GKGCMC	1 cut(s)	1639
BstUI	CGCG	2 cut(s)	20, 110
BstV1I	GCAGC	6 cut(s)	321, 461, 955, 1023, 1343, 1876
BstV2I	GAAGAC	2 cut(s)	1001, 1118
BstX2I	RGATCY	2 cut(s)	84, 795
BstXI	CCANNNNNNTGG	1 cut(s)	2075
BstYI	RGATCY	2 cut(s)	84, 795
BstZ17I	GTATAC	1 cut(s)	2242
BstZI	CGGCCG	1 cut(s)	390
BsuRI	GGCC	4 cut(s)	169, 392, 535, 2111
BtgZI	GCGATG	3 cut(s)	333, 360, 393
BtsCI	GGATG	3 cut(s)	424, 449, 1915
BtsI	GCAGTG	2 cut(s)	1087, 1615
BtsIMutI	CAGTG	4 cut(s)	11, 1087, 1615, 1746
BtuMI	TCGCGA	1 cut(s)	110
BveI	ACCTGC	1 cut(s)	1564
Cac8I	GCNNGC	8 cut(s)	22, 127, 131, 247, 720, 1078, 1647, 1787
CaiI	CAGNNNCTG	2 cut(s)	1188, 1459
CciI	TCATGA	1 cut(s)	712
CfoI	GCGC	1 cut(s)	690
Cfr13I	GGNCC	3 cut(s)	854, 2065, 2110
CseI	GACGC	6 cut(s)	193, 215, 246, 357, 851, 1445
CsiI	ACCWGGT	1 cut(s)	1381
Csp6I	GTAC	3 cut(s)	1263, 2151, 2278
CspCI	CAANNNNNGTGG	2 cut(s)	501, 536
CviQI	GTAC	3 cut(s)	1263, 2151, 2278
DdeI	CTNAG	5 cut(s)	188, 516, 783, 1092, 1100
DpnI	GATC	5 cut(s)	86, 213, 797, 1200, 2181
DpnII	GATC	5 cut(s)	84, 211, 795, 1198, 2179
DriI	GACNNNNNGTC	1 cut(s)	225
EaeI	YGGCCR	1 cut(s)	390
EagI	CGGCCG	1 cut(s)	390
Eam1104I	CTCTTC	2 cut(s)	181, 881
Eam1105I	GACNNNNNGTC	1 cut(s)	225
EarI	CTCTTC	2 cut(s)	181, 881
EclXI	CGGCCG	1 cut(s)	390
Eco130I	CCWWGG	4 cut(s)	357, 775, 1639, 2227
Eco147I	AGGCCT	1 cut(s)	169
Eco24I	GRGCYC	2 cut(s)	736, 907
Eco31I	GGTCTC	2 cut(s)	1215, 1372
Eco47I	GGWCC	2 cut(s)	854, 2065
Eco52I	CGGCCG	1 cut(s)	390
Eco57I	CTGAAG	1 cut(s)	564
Eco88I	CYCGRG	1 cut(s)	1947
EcoRI	GAATTC	1 cut(s)	465
EcoRII	CCWGG	5 cut(s)	856, 1216, 1315, 1381, 2002
EcoT14I	CCWWGG	4 cut(s)	357, 775, 1639, 2227
EcoT38I	GRGCYC	2 cut(s)	736, 907
ErhI	CCWWGG	4 cut(s)	357, 775, 1639, 2227
Esp3I	CGTCTC	2 cut(s)	391, 400
FalI	AAGNNNNNCTT	4 cut(s)	986, 1018, 1110, 1142
FaqI	GGGAC	3 cut(s)	1515, 1783, 2270
FauI	CCCGC	1 cut(s)	431
FauNDI	CATATG	1 cut(s)	1489
FblI	GTMKAC	1 cut(s)	2241
FokI	GGATG	3 cut(s)	431, 456, 1902
FriOI	GRGCYC	2 cut(s)	736, 907
FspBI	CTAG	5 cut(s)	470, 498, 752, 1418, 2223
GlaI	GCGC	1 cut(s)	689
GsaI	CCCAGC	2 cut(s)	989, 1043
GsuI	CTGGAG	5 cut(s)	1239, 1308, 1431, 1549, 1914
HaeIII	GGCC	4 cut(s)	169, 392, 535, 2111
HapII	CCGG	2 cut(s)	270, 2199
HgaI	GACGC	6 cut(s)	193, 215, 246, 357, 851, 1445
HhaI	GCGC	1 cut(s)	690
Hin1I	GRCGYC	3 cut(s)	226, 384, 862
Hin6I	GCGC	1 cut(s)	688
HinP1I	GCGC	1 cut(s)	688
HincII	GTYRAC	1 cut(s)	9
HindII	GTYRAC	1 cut(s)	9
HpaII	CCGG	2 cut(s)	270, 2199
HphI	GGTGA	5 cut(s)	40, 308, 549, 1400, 1753
Hpy166II	GTNNAC	4 cut(s)	9, 1544, 1744, 2242
Hpy188I	TCNGA	8 cut(s)	93, 219, 337, 544, 1156, 1776, 1867, 2184
Hpy8I	GTNNAC	4 cut(s)	9, 1544, 1744, 2242
Hpy99I	CGWCG	3 cut(s)	187, 231, 867
HpyAV	CCTTC	2 cut(s)	186, 2305
HpyCH4III	ACNGT	7 cut(s)	52, 341, 650, 955, 1184, 1226, 1750
HpyCH4IV	ACGT	3 cut(s)	384, 1546, 2280
HpyF10VI	GCNNNNNNNGC	7 cut(s)	78, 147, 246, 263, 315, 740, 1949
HpyF3I	CTNAG	5 cut(s)	188, 516, 783, 1092, 1100
HpySE526I	ACGT	3 cut(s)	384, 1546, 2280
Hsp92I	GRCGYC	3 cut(s)	226, 384, 862
HspAI	GCGC	1 cut(s)	688
Kzo9I	GATC	5 cut(s)	84, 211, 795, 1198, 2179
LmnI	GCTCC	5 cut(s)	195, 779, 910, 1450, 1685
Lsp1109I	GCAGC	6 cut(s)	321, 461, 955, 1023, 1343, 1876
LweI	GCATC	3 cut(s)	337, 2185, 2294
MabI	ACCWGGT	1 cut(s)	1381
MaeI	CTAG	5 cut(s)	470, 498, 752, 1418, 2223
MaeII	ACGT	3 cut(s)	384, 1546, 2280
MaeIII	GTNAC	4 cut(s)	52, 1303, 1795, 1828
MalI	GATC	5 cut(s)	86, 213, 797, 1200, 2181
MbiI	CCGCTC	1 cut(s)	1946
MboI	GATC	5 cut(s)	84, 211, 795, 1198, 2179
MfeI	CAATTG	1 cut(s)	525
MflI	RGATCY	2 cut(s)	84, 795
MhlI	GDGCHC	5 cut(s)	26, 736, 907, 1639, 1881
MmeI	TCCRAC	6 cut(s)	242, 403, 971, 1134, 2029, 2092
MroXI	GAANNNNTTC	1 cut(s)	998
MseI	TTAA	6 cut(s)	657, 897, 1146, 1956, 2211, 2326
MspA1I	CMGCKG	3 cut(s)	312, 1334, 1661
MspCI	CTTAAG	1 cut(s)	656
MspI	CCGG	2 cut(s)	270, 2199
MspR9I	CCNGG	5 cut(s)	858, 1218, 1317, 1383, 2004
MunI	CAATTG	1 cut(s)	525
Mva1269I	GAATGC	1 cut(s)	1028
MvaI	CCWGG	5 cut(s)	858, 1218, 1317, 1383, 2004
MvnI	CGCG	2 cut(s)	20, 110
MwoI	GCNNNNNNNGC	7 cut(s)	78, 147, 246, 263, 315, 740, 1949
NdeI	CATATG	1 cut(s)	1489
NdeII	GATC	5 cut(s)	84, 211, 795, 1198, 2179
NlaIV	GGNNCC	7 cut(s)	197, 797, 906, 1011, 1446, 1687, 2066
NmeAIII	GCCGAG	1 cut(s)	368
NmuCI	GTSAC	2 cut(s)	1303, 1795
NruI	TCGCGA	1 cut(s)	110
NspI	RCATGY	3 cut(s)	438, 1823, 2248
PagI	TCATGA	1 cut(s)	712
PceI	AGGCCT	1 cut(s)	169
PcsI	WCGNNNNNNNCGW	3 cut(s)	390, 395, 2277
PctI	GAATGC	1 cut(s)	1028
PdmI	GAANNNNTTC	1 cut(s)	998
PfeI	GAWTC	5 cut(s)	635, 819, 1048, 1901, 2089
PflFI	GACNNNGTC	1 cut(s)	2063
PflMI	CCANNNNNTGG	2 cut(s)	1238, 1459
PshAI	GACNNNNGTC	1 cut(s)	404
Psp6I	CCWGG	5 cut(s)	856, 1216, 1315, 1381, 2002
PspFI	CCCAGC	2 cut(s)	985, 1039
PspGI	CCWGG	5 cut(s)	856, 1216, 1315, 1381, 2002
PspN4I	GGNNCC	7 cut(s)	197, 797, 906, 1011, 1446, 1687, 2066
PspPI	GGNCC	3 cut(s)	854, 2065, 2110
PstNI	CAGNNNCTG	2 cut(s)	1188, 1459
PsuI	RGATCY	2 cut(s)	84, 795
PsyI	GACNNNGTC	1 cut(s)	2063
PvuII	CAGCTG	2 cut(s)	1334, 1661
RruI	TCGCGA	1 cut(s)	110
RsaI	GTAC	3 cut(s)	1264, 2152, 2279
RsaNI	GTAC	3 cut(s)	1263, 2151, 2278
SaqAI	TTAA	6 cut(s)	657, 897, 1146, 1956, 2211, 2326
Sau3AI	GATC	5 cut(s)	84, 211, 795, 1198, 2179
Sau96I	GGNCC	3 cut(s)	854, 2065, 2110
ScaI	AGTACT	1 cut(s)	2152
ScrFI	CCNGG	5 cut(s)	858, 1218, 1317, 1383, 2004
SduI	GDGCHC	5 cut(s)	26, 736, 907, 1639, 1881
SexAI	ACCWGGT	1 cut(s)	1381
SfaNI	GCATC	3 cut(s)	337, 2185, 2294
SfcI	CTRYAG	4 cut(s)	1656, 1749, 2016, 2289
SinI	GGWCC	2 cut(s)	854, 2065
SmlI	CTYRAG	3 cut(s)	656, 1871, 1880
SmoI	CTYRAG	3 cut(s)	656, 1871, 1880
SseBI	AGGCCT	1 cut(s)	169
SspMI	CTAG	5 cut(s)	470, 498, 752, 1418, 2223
StuI	AGGCCT	1 cut(s)	169
StyD4I	CCNGG	5 cut(s)	856, 1216, 1315, 1381, 2002
StyI	CCWWGG	4 cut(s)	357, 775, 1639, 2227
TaaI	ACNGT	7 cut(s)	52, 341, 650, 955, 1184, 1226, 1750
TaiI	ACGT	3 cut(s)	387, 1549, 2283
TaqI	TCGA	5 cut(s)	29, 115, 182, 398, 1736
TatI	WGTACW	1 cut(s)	2150
TauI	GCSGC	5 cut(s)	153, 315, 318, 1514, 1946
TfiI	GAWTC	5 cut(s)	635, 819, 1048, 1901, 2089
Tru1I	TTAA	6 cut(s)	657, 897, 1146, 1956, 2211, 2326
Tru9I	TTAA	6 cut(s)	657, 897, 1146, 1956, 2211, 2326
TscAI	CASTG	4 cut(s)	18, 1087, 1615, 1753
TseFI	GTSAC	2 cut(s)	1303, 1795
TseI	GCWGC	6 cut(s)	309, 449, 968, 1036, 1331, 1889
Tsp45I	GTSAC	2 cut(s)	1303, 1795
TspDTI	ATGAA	8 cut(s)	146, 168, 780, 797, 929, 936, 951, 1904
TspRI	CASTG	4 cut(s)	18, 1087, 1615, 1753
Tth111I	GACNNNGTC	1 cut(s)	2063
Van91I	CCANNNNNTGG	2 cut(s)	1238, 1459
Vha464I	CTTAAG	1 cut(s)	656
VpaK11BI	GGWCC	2 cut(s)	854, 2065
XapI	RAATTY	3 cut(s)	465, 1576, 1803
XbaI	TCTAGA	1 cut(s)	469
XceI	RCATGY	3 cut(s)	438, 1823, 2248
XcmI	CCANNNNNNNNNTGG	1 cut(s)	987
XmiI	GTMKAC	1 cut(s)	2241
XmnI	GAANNNNTTC	1 cut(s)	998
XspI	CTAG	5 cut(s)	470, 498, 752, 1418, 2223
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	1 cut(s)	2152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.