Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006583

Physical Location & Seq

Genomic Coordinates Forward (+)

4270394 .. 4285507

Gene Structure 15114 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03740.12

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2874 bp

ATGTCAATGGAGGCCACATTGTCCGCCGCGACGCCCACAACGGCAGCTCTCCTTGCCGCCTCCAAATCCCTCAACCGCCGACTCGCCAGGACCTTCCTCAGTCAACGCCTCCCCAGTCGTCCCCTTGCTCGCAGGCACCTCACTTCCCTTCACACCAACTACTACGGCTTTCAGACCCCTAAACCTAATCCTTTCTCTTCCTCGTCTCTGAGGAGTCTCTGCGTCGTTGATCCGCTCTTACGGCGCCGTTTAGAGTGTGTTGGTAGCTCCGCTGCTTCATTTGCCTCTGGTGGTGGAAATGGTGGCTTTGGCGGAGAGAGTGGCGGCAGCGGCGGTGACGGCGAGGGAGGTTCCGGCGGCGGCGATGCCAAGTCCAAGTTTGTTGCTGCTGGAGGCGATGAGGTTTCGGTGCTCTCCTCTGATGTCATTATTCTCGATGTTGGAGGAATGACATGTGGGGGATGTGCAGCCAGTGTGAAGCGAATTCTAGAAAATCAACCTCAGGTGTCGTCTGCGAGTGTAAATCTCACAACAGAGACGGCAATTGTGTGGCCTGTGTCCGAAGCGAAGGCTGCACCAAACTGGCAAAAGCAGCTAGGAGAGACACTTGCAAACCAATTGACCAGTTGCGGGTTCAAGTCTAACCTTCGTGATTCAGGACGAGACAGTTTTCTCAAAGTTTTTGAAAGGAAAATGGAAGAAAAGCGTAAACGCCTAAAAGAAAGTGGAAATGAGCTTGCATTCTCTTGGGCTCTGTGTGCTGTCTGCCTAATTGGCCATGCATCTCATTTCTTTGGGGCTAAGGCACCATGGATCCATGCGCTTCATTCGACAGGATTCCATTTGTCCTTATGTCTATTTACATTGCTTGGTCCTGGGCGTCGACTTATCTTTGATGGTTTGAGGAGTCTTGTTAAAGGGGCTCCAAACATGAATACTTTGGTTGGTCTAGGGGCCTTATCGTCATTTACTGTCAGTTCATTAGCTGCCTTTATACCAAAACTGGGCTGGAAGACTTTCTTCGAGGAACCAATTATGTTGATAGCATTCGTCTTGTTAGGGAGGAATCTTGAACAAAGAGCTAAAATCAAAGCAAGCAGTGATATGACTGAACTTCTTAGTATTGTACCGTCAAAAGCACGTCTTTTGGTCAATAACGGTGAACAAGAGTTGGAATCAGTAGTTGAAGTTTCTACTAACAGCCTCTCTGTTGGAGATAAAATTGTTGTACTACCTGGAGACCGTGTTCCAGTAGATGGAATTGTTAAAGCTGGTAGGAGTATCATTGATGAGTCAAGTTTTACAGGAGAGCCGTTGCCTGTAACCAAACTACCTGGGAGTCAAGTTGCCGCCGGAAGCATAAATCTCAATGGCACTCTTACAGTTGAAGTACAGAGACCAGGTGGTGAGACTGCTATGGCAGACATTGTTCGCTTGGTAGAAGAAGCACAGAGTAGGGAAGCTCCTGTACAGCGCTTGGCCGACAAGGTGTCTGGACATTTTACATATGGAGTAATGACACTCTCTGCTGCTACATTTCTATTTTGGAGCTTGATTGGTGGTCATATTTTACCTGCTGCTTTTCACGGAGGAAATTCAGTTTCGTTAGCATTGCAACTCTCTTGCAGTGTACTGGTTGTTGCTTGTCCATGTGCTCTTGGGCTAGCCACACCTACAGCTGTGCTGGTTGGGACTTCGTTGGGAGCTAAAAGAGGACTACTTTTGCGTGGCGGTAGTATTTTAGAGAAGTTTTCAATGGTGAACACTATAGTGTTTGATAAAACAGGGACTCTGACAGTGGGCAAACCTGTTGTGACTAATATTGTGACTCCTGAACGTTCGAAAGTTACTGACTTAAAAGAAAAGATAAACCATACTTGGTCAGAAGTTGATGTTCTGAAGTTTGCTGCTGGAGTAGAATCGAATACAGTTCATCCTGTTGCAAAAGCTATTGTGGAAGCTAGTCAGGCTCTTAATTGTCAGAATATGAAGGTTGCGGATGGAACATTCTTGGAAGAACCTGGCTCTGGTGCTGTAGCAACTATTGAGAACAAAAAGGTTTCTGTTGGGACTTTGGAATGGGTTCAAAGGCATGGAGTTACTGAGAATCCATTTGAAGAAGTAGAAGCCCATACAAGTCAATCTATTGTATATGTTGGCATTGATAGTATTCTTGCCGGTCTCATTTATTTTGAGGATCAGATAAGGGAAGATGCTGGACAAGTTGTTAAATCTTTATCTAAGCAAGGGATCGATGTATACATGCTATCTGGGGACAAAAGAAATAATGCGGAGTACGTCGCGTCTGCTGTTGGTATTCCGAAAGAGAAGGTGATATCTGGTGTTAAACCGACAGAAAAGAAGAAGTTCATCATGGAACTTCAGAAGGATCAGAACATTGTAGCAATGGTTGGTGATGGAATTAATGATGCTGCAGCATTGGCTTCATCGCATGTTGGAATTGCCATGGGTGGTGGTGTTGGAGCTGCTAGTGAGGTGTCTTCTATTGTGCTATTGGGCAACAGACTTTCACAGTTACTTGATGCATTGGAGCTGAGTAGGCTAACTATGAAGACTGTGAAGCAAAATCTGTGGTGGGCTTTTGCATACAATATTGTAGGACTTCCAATTGCTGCTGGAGTTTTGCTTCCAGTAACTGGGACCATTCTGACGCCGTCAATTGCTGGAGGCCTCATGGGCTTGAGTTCTGTAGGGGTTATGGCAAATTCATTGTTTCTGAGATACAAGTTCTCTAAACAGGAAAAAAAATATAGTGCATCTGCTCGCTCCGAAACTAATGAGGAATCTGATCTTCTCATCGACGTAAGTGCAGAAGAAGAGCATCCTCCTTCTGATGGTAAATGGAAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008152 GO:0008324 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009767 GO:0009941 GO:0009987 GO:0015075 GO:0015318 GO:0015979 GO:0016530 GO:0016531 GO:0019684 GO:0022857 GO:0022890 GO:0022900 GO:0030001 GO:0031967 GO:0031975 GO:0034220 GO:0035434 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046873 GO:0046914 GO:0046915 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055070 GO:0055076 GO:0055080 GO:0055085 GO:0055114 GO:0065007 GO:0065008 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0140104

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

958

Amino Acids

101.26

Weight (kDa)

7.88

Isoelectric Point (pI)

37.86

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33520 AT4G33520
fragaria_vesca	FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930 FvH4_2g11930
malus_domestica	MD05G1044900.v1.1
prunus_persica	Prupe.8G060400_v2.0.a1
pyrus_communis	pycom05g03740
rosa_chinensis	RchiOBHm_Chr6g0271821
rosa_laevigata	RLG00000013712
rosa_multiflora	Rmu_sc0002396.1_g000018
rosa_roxburghii	Rroxscaffold_173G00435020 Rroxscaffold_7G00196710
rosa_rugosa	Rorug06G0063300 Rorug06G0063400
rosa_samantha	Rh6AG181500 Rh6BG186000 Rh6CG182200 Rh6DG173300
rosa_wichuraiana	Rw6G015660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1582
AccB1I	GGYRCC	3 cut(s)	135, 243, 805
AccB7I	CCANNNNNTGG	2 cut(s)	1256, 2657
AccBSI	CCGCTC	1 cut(s)	235
AccI	GTMKAC	2 cut(s)	883, 2259
AccII	CGCG	2 cut(s)	29, 2303
AclI	AACGTT	1 cut(s)	1837
AclWI	GGATC	6 cut(s)	224, 808, 821, 2205, 2258, 2397
AcoI	YGGCCR	2 cut(s)	775, 1479
AcsI	RAATTY	3 cut(s)	483, 1594, 2725
AcuI	CTGAAG	2 cut(s)	1919, 2366
AcyI	GRCGYC	4 cut(s)	32, 244, 880, 2672
AfaI	GTAC	6 cut(s)	1128, 1230, 1392, 1470, 1632, 2297
AfeI	AGCGCT	1 cut(s)	1475
AfiI	CCNNNNNNNGG	6 cut(s)	630, 1004, 1256, 2027, 2657, 2855
AflIII	ACRYGT	1 cut(s)	452
AgsI	TTSAA	8 cut(s)	637, 686, 1073, 1187, 1388, 1755, 2087, 2117
AhdI	GACNNNNNGTC	1 cut(s)	1489
AjiI	CACGTC	1 cut(s)	1142
AjnI	CCWGG	6 cut(s)	86, 874, 1234, 1333, 1399, 2020
AleI	CACNNNNGTG	1 cut(s)	1769
AloI	GAACNNNNNNTCC	2 cut(s)	1230, 1262
Alw21I	GWGCWC	2 cut(s)	414, 1657
Alw26I	GTCTC	9 cut(s)	210, 221, 530, 596, 657, 1233, 1390, 1403, 2186
AlwI	GGATC	6 cut(s)	224, 808, 821, 2205, 2258, 2397
AlwNI	CAGNNNCTG	1 cut(s)	2657
Aor51HI	AGCGCT	1 cut(s)	1475
AoxI	GGCC	6 cut(s)	12, 551, 775, 954, 1479, 2689
ApoI	RAATTY	3 cut(s)	483, 1594, 2725
AseI	ATTAAT	1 cut(s)	2424
Asp700I	GAANNNNTTC	3 cut(s)	1016, 2082, 2366
AspLEI	GCGC	3 cut(s)	246, 823, 1476
AspS9I	GGNCC	4 cut(s)	90, 872, 954, 2661
AsuHPI	GGTGA	6 cut(s)	347, 1172, 1418, 1771, 2344, 2426
AsuII	TTCGAA	1 cut(s)	1841
AsuNHI	GCTAGC	1 cut(s)	1663
AvaII	GGWCC	3 cut(s)	90, 872, 2661
AxyI	CCTNAGG	1 cut(s)	501
BalI	TGGCCA	1 cut(s)	777
BamHI	GGATCC	1 cut(s)	813
BanI	GGYRCC	3 cut(s)	135, 243, 805
BanII	GRGCYC	2 cut(s)	754, 925
BarI	GAAGNNNNNNTAC	2 cut(s)	1452, 1484
BbsI	GAAGAC	3 cut(s)	1019, 2493, 2579
Bbv12I	GWGCWC	2 cut(s)	414, 1657
BccI	CCATC	5 cut(s)	890, 1250, 1994, 2411, 2849
BceAI	ACGGC	8 cut(s)	57, 181, 231, 257, 355, 555, 1297, 2659
BcgI	CGANNNNNNTGC	2 cut(s)	2810, 2844
BciT130I	CCWGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
BcoDI	GTCTC	9 cut(s)	210, 221, 530, 596, 657, 1233, 1390, 1403, 2186
BfaI	CTAG	6 cut(s)	488, 596, 950, 1664, 1962, 2490
BfmI	CTRYAG	5 cut(s)	1674, 1767, 2034, 2433, 2709
BfoI	RGCGCY	2 cut(s)	247, 1477
BfuAI	ACCTGC	1 cut(s)	1582
BglI	GCCNNNNNGGC	2 cut(s)	774, 2697
Bme1390I	CCNGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
Bme18I	GGWCC	3 cut(s)	90, 872, 2661
BmeRI	GACNNNNNGTC	1 cut(s)	1489
BmgBI	CACGTC	1 cut(s)	1142
BmgT120I	GGNCC	4 cut(s)	90, 872, 954, 2661
BmiI	GGNNCC	9 cut(s)	137, 245, 352, 807, 815, 924, 955, 1029, 2662
BmrFI	CCNGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
BmrI	ACTGGG	3 cut(s)	108, 1013, 2667
BmsI	GCATC	7 cut(s)	355, 791, 2203, 2419, 2533, 2786, 2851
BmtI	GCTAGC	1 cut(s)	1667
BmuI	ACTGGG	3 cut(s)	108, 1013, 2667
BpiI	GAAGAC	3 cut(s)	1019, 2493, 2579
BpmI	CTGGAG	5 cut(s)	411, 1257, 1932, 2658, 2706
Bpu10I	CCTNAGC	1 cut(s)	801
Bpu14I	TTCGAA	1 cut(s)	1841
BpuEI	CTTGAG	1 cut(s)	2722
Bsa29I	ATCGAT	1 cut(s)	2253
BsaHI	GRCGYC	4 cut(s)	32, 244, 880, 2672
BsaI	GGTCTC	3 cut(s)	1233, 1390, 2186
BsaJI	CCNNGG	4 cut(s)	809, 875, 1334, 2466
BsaXI	ACNNNNNCTCC	2 cut(s)	1230, 1260
Bsc4I	CCNNNNNNNGG	6 cut(s)	630, 1004, 1256, 2027, 2657, 2855
Bse118I	RCCGGY	1 cut(s)	2177
Bse1I	ACTGG	9 cut(s)	114, 471, 587, 624, 1008, 1250, 1638, 2651, 2662
Bse21I	CCTNAGG	1 cut(s)	501
Bse3DI	GCAATG	3 cut(s)	863, 1610, 2412
BseBI	CCWGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
BseCI	ATCGAT	1 cut(s)	2253
BseDI	CCNNGG	4 cut(s)	809, 875, 1334, 2466
BseGI	GGATG	4 cut(s)	467, 1933, 2005, 2842
BseLI	CCNNNNNNNGG	6 cut(s)	630, 1004, 1256, 2027, 2657, 2855
BseMI	GCAATG	3 cut(s)	863, 1610, 2412
BseMII	CTCAG	6 cut(s)	112, 200, 515, 2094, 2546, 2729
BseNI	ACTGG	9 cut(s)	114, 471, 587, 624, 1008, 1250, 1638, 2651, 2662
BseRI	GAGGAG	3 cut(s)	226, 406, 919
BsgI	GTGCAG	3 cut(s)	486, 558, 2850
Bsh1236I	CGCG	2 cut(s)	29, 2303
BshFI	GGCC	6 cut(s)	14, 553, 777, 956, 1481, 2691
BshNI	GGYRCC	3 cut(s)	135, 243, 805
BshVI	ATCGAT	1 cut(s)	2253
BsiHKAI	GWGCWC	2 cut(s)	414, 1657
BsiSI	CCGG	3 cut(s)	354, 1353, 2178
BslFI	GGGAC	6 cut(s)	105, 1705, 1801, 2083, 2288, 2674
BslI	CCNNNNNNNGG	6 cut(s)	630, 1004, 1256, 2027, 2657, 2855
BsmAI	GTCTC	9 cut(s)	210, 221, 530, 596, 657, 1233, 1390, 1403, 2186
BsmBI	CGTCTC	2 cut(s)	210, 530
BsmFI	GGGAC	6 cut(s)	105, 1705, 1801, 2083, 2288, 2674
BsmI	GAATGC	2 cut(s)	740, 1046
BsnI	GGCC	6 cut(s)	14, 553, 777, 956, 1481, 2691
Bso31I	GGTCTC	3 cut(s)	1233, 1390, 2186
Bsp119I	TTCGAA	1 cut(s)	1841
Bsp1286I	GDGCHC	4 cut(s)	414, 754, 925, 1657
Bsp1407I	TGTACA	1 cut(s)	1468
Bsp143I	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
Bsp19I	CCATGG	2 cut(s)	809, 2466
BspANI	GGCC	6 cut(s)	14, 553, 777, 956, 1481, 2691
BspCNI	CTCAG	6 cut(s)	111, 201, 514, 2095, 2547, 2730
BspDI	ATCGAT	1 cut(s)	2253
BspFNI	CGCG	2 cut(s)	29, 2303
BspLI	GGNNCC	9 cut(s)	137, 245, 352, 807, 815, 924, 955, 1029, 2662
BspMAI	CTGCAG	1 cut(s)	2437
BspMI	ACCTGC	1 cut(s)	1582
BspOI	GCTAGC	1 cut(s)	1667
BspPI	GGATC	6 cut(s)	224, 808, 821, 2205, 2258, 2397
BspQI	GCTCTTC	1 cut(s)	2832
BspT104I	TTCGAA	1 cut(s)	1841
BspT107I	GGYRCC	3 cut(s)	135, 243, 805
BspTNI	GGTCTC	3 cut(s)	1233, 1390, 2186
BsrBI	CCGCTC	1 cut(s)	235
BsrDI	GCAATG	3 cut(s)	863, 1610, 2412
BsrFI	RCCGGY	1 cut(s)	2177
BsrGI	TGTACA	1 cut(s)	1468
BsrI	ACTGG	9 cut(s)	114, 471, 587, 624, 1008, 1250, 1638, 2651, 2662
BssAI	RCCGGY	1 cut(s)	2177
BssECI	CCNNGG	4 cut(s)	809, 875, 1334, 2466
BssMI	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
BssNAI	GTATAC	1 cut(s)	2260
BssNI	GRCGYC	4 cut(s)	32, 244, 880, 2672
BssT1I	CCWWGG	2 cut(s)	809, 2466
Bst1107I	GTATAC	1 cut(s)	2260
Bst2UI	CCWGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
Bst6I	CTCTTC	2 cut(s)	202, 2832
BstACI	GRCGYC	4 cut(s)	32, 244, 880, 2672
BstAUI	TGTACA	1 cut(s)	1468
BstBI	TTCGAA	1 cut(s)	1841
BstC8I	GCNNGC	6 cut(s)	130, 134, 738, 1096, 1665, 2785
BstDEI	CTNAG	9 cut(s)	98, 209, 501, 801, 1118, 2103, 2241, 2555, 2738
BstDSI	CCRYGG	2 cut(s)	809, 2466
BstF5I	GGATG	4 cut(s)	467, 1933, 2005, 2842
BstFNI	CGCG	2 cut(s)	29, 2303
BstH2I	RGCGCY	2 cut(s)	247, 1477
BstHHI	GCGC	3 cut(s)	246, 823, 1476
BstKTI	GATC	6 cut(s)	232, 816, 2200, 2253, 2392, 2812
BstMAI	GTCTC	9 cut(s)	210, 221, 530, 596, 657, 1233, 1390, 1403, 2186
BstMBI	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
BstNI	CCWGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
BstNSI	RCATGY	3 cut(s)	456, 2266, 2456
BstSCI	CCNGG	6 cut(s)	86, 874, 1234, 1333, 1399, 2020
BstSFI	CTRYAG	5 cut(s)	1674, 1767, 2034, 2433, 2709
BstUI	CGCG	2 cut(s)	29, 2303
BstV2I	GAAGAC	3 cut(s)	1019, 2493, 2579
BstX2I	RGATCY	1 cut(s)	813
BstYI	RGATCY	1 cut(s)	813
BstZ17I	GTATAC	1 cut(s)	2260
Bsu15I	ATCGAT	1 cut(s)	2253
Bsu36I	CCTNAGG	1 cut(s)	501
BsuRI	GGCC	6 cut(s)	14, 553, 777, 956, 1481, 2691
BsuTUI	ATCGAT	1 cut(s)	2253
BtgI	CCRYGG	2 cut(s)	809, 2466
BtgZI	GCGATG	3 cut(s)	378, 411, 2433
BtrI	CACGTC	1 cut(s)	1142
BtsCI	GGATG	4 cut(s)	467, 1933, 2005, 2842
BtsI	GCAGTG	2 cut(s)	1105, 1633
BtsIMutI	CAGTG	4 cut(s)	478, 1105, 1633, 1803
BveI	ACCTGC	1 cut(s)	1582
Cac8I	GCNNGC	6 cut(s)	130, 134, 738, 1096, 1665, 2785
CaiI	CAGNNNCTG	1 cut(s)	2657
CfoI	GCGC	3 cut(s)	246, 823, 1476
Cfr10I	RCCGGY	1 cut(s)	2177
Cfr13I	GGNCC	4 cut(s)	90, 872, 954, 2661
ClaI	ATCGAT	1 cut(s)	2253
CseI	GACGC	5 cut(s)	40, 211, 869, 2292, 2680
CsiI	ACCWGGT	1 cut(s)	1399
Csp6I	GTAC	6 cut(s)	1127, 1229, 1391, 1469, 1631, 2296
CspCI	CAANNNNNGTGG	2 cut(s)	2573, 2608
CviQI	GTAC	6 cut(s)	1127, 1229, 1391, 1469, 1631, 2296
DdeI	CTNAG	9 cut(s)	98, 209, 501, 801, 1118, 2103, 2241, 2555, 2738
DinI	GGCGCC	1 cut(s)	245
DpnI	GATC	6 cut(s)	231, 815, 2199, 2252, 2391, 2811
DpnII	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
DriI	GACNNNNNGTC	1 cut(s)	1489
EaeI	YGGCCR	2 cut(s)	775, 1479
Eam1104I	CTCTTC	2 cut(s)	202, 2832
Eam1105I	GACNNNNNGTC	1 cut(s)	1489
EarI	CTCTTC	2 cut(s)	202, 2832
EciI	GGCGGA	2 cut(s)	13, 327
Eco130I	CCWWGG	2 cut(s)	809, 2466
Eco147I	AGGCCT	1 cut(s)	2691
Eco24I	GRGCYC	2 cut(s)	754, 925
Eco31I	GGTCTC	3 cut(s)	1233, 1390, 2186
Eco32I	GATATC	1 cut(s)	2337
Eco47I	GGWCC	3 cut(s)	90, 872, 2661
Eco47III	AGCGCT	1 cut(s)	1475
Eco57I	CTGAAG	2 cut(s)	1919, 2366
Eco81I	CCTNAGG	1 cut(s)	501
EcoO109I	RGGNCCY	2 cut(s)	90, 954
EcoRI	GAATTC	1 cut(s)	483
EcoRII	CCWGG	6 cut(s)	86, 874, 1234, 1333, 1399, 2020
EcoRV	GATATC	1 cut(s)	2337
EcoT14I	CCWWGG	2 cut(s)	809, 2466
EcoT22I	ATGCAT	2 cut(s)	784, 2548
EcoT38I	GRGCYC	2 cut(s)	754, 925
EgeI	GGCGCC	1 cut(s)	245
EheI	GGCGCC	1 cut(s)	245
ErhI	CCWWGG	2 cut(s)	809, 2466
Esp3I	CGTCTC	2 cut(s)	210, 530
FalI	AAGNNNNNCTT	4 cut(s)	1004, 1036, 1128, 1160
FaqI	GGGAC	6 cut(s)	105, 1705, 1801, 2083, 2288, 2674
FauI	CCCGC	1 cut(s)	623
FauNDI	CATATG	1 cut(s)	1507
FblI	GTMKAC	2 cut(s)	883, 2259
FokI	GGATG	4 cut(s)	474, 1920, 2012, 2829
FriOI	GRGCYC	2 cut(s)	754, 925
FspBI	CTAG	6 cut(s)	488, 596, 950, 1664, 1962, 2490
GlaI	GCGC	3 cut(s)	245, 822, 1475
GsuI	CTGGAG	5 cut(s)	411, 1257, 1932, 2658, 2706
HaeII	RGCGCY	2 cut(s)	247, 1477
HaeIII	GGCC	6 cut(s)	14, 553, 777, 956, 1481, 2691
HapII	CCGG	3 cut(s)	354, 1353, 2178
HgaI	GACGC	5 cut(s)	40, 211, 869, 2292, 2680
HhaI	GCGC	3 cut(s)	246, 823, 1476
Hin1I	GRCGYC	4 cut(s)	32, 244, 880, 2672
Hin6I	GCGC	3 cut(s)	244, 821, 1474
HinP1I	GCGC	3 cut(s)	244, 821, 1474
HincII	GTYRAC	2 cut(s)	104, 884
HindII	GTYRAC	2 cut(s)	104, 884
HpaII	CCGG	3 cut(s)	354, 1353, 2178
HphI	GGTGA	6 cut(s)	347, 1172, 1418, 1771, 2344, 2426
Hpy166II	GTNNAC	7 cut(s)	104, 710, 884, 1163, 1631, 1762, 2260
Hpy188III	TCNNGA	7 cut(s)	434, 488, 650, 657, 1070, 1494, 1832
Hpy8I	GTNNAC	7 cut(s)	104, 710, 884, 1163, 1631, 1762, 2260
Hpy99I	CGWCG	5 cut(s)	34, 227, 885, 2303, 2825
HpyAV	CCTTC	8 cut(s)	103, 158, 562, 656, 1984, 2323, 2380, 2859
HpyCH4IV	ACGT	4 cut(s)	1141, 1837, 2298, 2823
HpyF3I	CTNAG	9 cut(s)	98, 209, 501, 801, 1118, 2103, 2241, 2555, 2738
HpySE526I	ACGT	4 cut(s)	1141, 1837, 2298, 2823
Hsp92I	GRCGYC	4 cut(s)	32, 244, 880, 2672
HspAI	GCGC	3 cut(s)	244, 821, 1474
KasI	GGCGCC	1 cut(s)	243
Kzo9I	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
LguI	GCTCTTC	1 cut(s)	2832
LmnI	GCTCC	8 cut(s)	272, 928, 1468, 1548, 1703, 2483, 2551, 2792
LweI	GCATC	7 cut(s)	355, 791, 2203, 2419, 2533, 2786, 2851
MabI	ACCWGGT	1 cut(s)	1399
MaeI	CTAG	6 cut(s)	488, 596, 950, 1664, 1962, 2490
MaeII	ACGT	4 cut(s)	1141, 1837, 2298, 2823
MaeIII	GTNAC	8 cut(s)	335, 1321, 1813, 1825, 1846, 2098, 2535, 2653
MalI	GATC	6 cut(s)	231, 815, 2199, 2252, 2391, 2811
MbiI	CCGCTC	1 cut(s)	235
MboI	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
MfeI	CAATTG	4 cut(s)	543, 617, 2628, 2679
MflI	RGATCY	1 cut(s)	813
MhlI	GDGCHC	4 cut(s)	414, 754, 925, 1657
MlsI	TGGCCA	1 cut(s)	777
MluNI	TGGCCA	1 cut(s)	777
Mly113I	GGCGCC	1 cut(s)	244
MlyI	GAGTC	7 cut(s)	75, 223, 916, 1301, 1348, 1783, 1822
MmeI	TCCRAC	5 cut(s)	421, 1152, 1192, 2437, 2461
Mox20I	TGGCCA	1 cut(s)	777
Mph1103I	ATGCAT	2 cut(s)	784, 2548
MroXI	GAANNNNTTC	3 cut(s)	1016, 2082, 2366
MscI	TGGCCA	1 cut(s)	777
MseI	TTAA	7 cut(s)	915, 1266, 1856, 1974, 2229, 2346, 2424
MslI	CAYNNNNRTG	1 cut(s)	1769
Msp20I	TGGCCA	1 cut(s)	777
MspA1I	CMGCKG	3 cut(s)	272, 330, 1679
MspI	CCGG	3 cut(s)	354, 1353, 2178
MspR9I	CCNGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
MunI	CAATTG	4 cut(s)	543, 617, 2628, 2679
Mva1269I	GAATGC	2 cut(s)	740, 1046
MvaI	CCWGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
MvnI	CGCG	2 cut(s)	29, 2303
NarI	GGCGCC	1 cut(s)	244
NcoI	CCATGG	2 cut(s)	809, 2466
NdeI	CATATG	1 cut(s)	1507
NdeII	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
NheI	GCTAGC	1 cut(s)	1663
NlaIV	GGNNCC	9 cut(s)	137, 245, 352, 807, 815, 924, 955, 1029, 2662
NmuCI	GTSAC	3 cut(s)	335, 1813, 1825
NsiI	ATGCAT	2 cut(s)	784, 2548
NspI	RCATGY	3 cut(s)	456, 2266, 2456
NspV	TTCGAA	1 cut(s)	1841
OliI	CACNNNNGTG	1 cut(s)	1769
PceI	AGGCCT	1 cut(s)	2691
PciI	ACATGT	1 cut(s)	452
PciSI	GCTCTTC	1 cut(s)	2832
PctI	GAATGC	2 cut(s)	740, 1046
PdmI	GAANNNNTTC	3 cut(s)	1016, 2082, 2366
PfeI	GAWTC	7 cut(s)	653, 837, 1066, 1175, 1919, 2107, 2804
PflFI	GACNNNGTC	1 cut(s)	2674
PflMI	CCANNNNNTGG	2 cut(s)	1256, 2657
PleI	GAGTC	7 cut(s)	75, 222, 915, 1300, 1347, 1783, 1822
PluTI	GGCGCC	1 cut(s)	247
PpsI	GAGTC	7 cut(s)	75, 222, 915, 1300, 1347, 1783, 1822
PpuMI	RGGWCCY	1 cut(s)	90
PscI	ACATGT	1 cut(s)	452
PshBI	ATTAAT	1 cut(s)	2424
Psp1406I	AACGTT	1 cut(s)	1837
Psp5II	RGGWCCY	1 cut(s)	90
Psp6I	CCWGG	6 cut(s)	86, 874, 1234, 1333, 1399, 2020
PspGI	CCWGG	6 cut(s)	86, 874, 1234, 1333, 1399, 2020
PspN4I	GGNNCC	9 cut(s)	137, 245, 352, 807, 815, 924, 955, 1029, 2662
PspPI	GGNCC	4 cut(s)	90, 872, 954, 2661
PspPPI	RGGWCCY	1 cut(s)	90
PstI	CTGCAG	1 cut(s)	2437
PstNI	CAGNNNCTG	1 cut(s)	2657
PsuI	RGATCY	1 cut(s)	813
PsyI	GACNNNGTC	1 cut(s)	2674
PvuII	CAGCTG	1 cut(s)	1679
RsaI	GTAC	6 cut(s)	1128, 1230, 1392, 1470, 1632, 2297
RsaNI	GTAC	6 cut(s)	1127, 1229, 1391, 1469, 1631, 2296
RseI	CAYNNNNRTG	1 cut(s)	1769
SalI	GTCGAC	1 cut(s)	882
SapI	GCTCTTC	1 cut(s)	2832
SaqAI	TTAA	7 cut(s)	915, 1266, 1856, 1974, 2229, 2346, 2424
Sau3AI	GATC	6 cut(s)	229, 813, 2197, 2250, 2389, 2809
Sau96I	GGNCC	4 cut(s)	90, 872, 954, 2661
SchI	GAGTC	7 cut(s)	75, 223, 916, 1301, 1348, 1783, 1822
ScrFI	CCNGG	6 cut(s)	88, 876, 1236, 1335, 1401, 2022
SduI	GDGCHC	4 cut(s)	414, 754, 925, 1657
SexAI	ACCWGGT	1 cut(s)	1399
SfaNI	GCATC	7 cut(s)	355, 791, 2203, 2419, 2533, 2786, 2851
SfcI	CTRYAG	5 cut(s)	1674, 1767, 2034, 2433, 2709
SfoI	GGCGCC	1 cut(s)	245
SfuI	TTCGAA	1 cut(s)	1841
SinI	GGWCC	3 cut(s)	90, 872, 2661
SmiMI	CAYNNNNRTG	1 cut(s)	1769
SmlI	CTYRAG	1 cut(s)	2701
SmoI	CTYRAG	1 cut(s)	2701
SseBI	AGGCCT	1 cut(s)	2691
SspDI	GGCGCC	1 cut(s)	243
SspI	AATATT	2 cut(s)	1822, 2614
SspMI	CTAG	6 cut(s)	488, 596, 950, 1664, 1962, 2490
StuI	AGGCCT	1 cut(s)	2691
StyD4I	CCNGG	6 cut(s)	86, 874, 1234, 1333, 1399, 2020
StyI	CCWWGG	2 cut(s)	809, 2466
TaiI	ACGT	4 cut(s)	1144, 1840, 2301, 2826
TaqI	TCGA	8 cut(s)	435, 830, 883, 1023, 1841, 1922, 2253, 2820
TatI	WGTACW	4 cut(s)	1228, 1390, 1468, 1630
TauI	GCSGC	7 cut(s)	29, 59, 327, 333, 360, 363, 1352
TfiI	GAWTC	7 cut(s)	653, 837, 1066, 1175, 1919, 2107, 2804
Tru1I	TTAA	7 cut(s)	915, 1266, 1856, 1974, 2229, 2346, 2424
Tru9I	TTAA	7 cut(s)	915, 1266, 1856, 1974, 2229, 2346, 2424
TscAI	CASTG	4 cut(s)	478, 1105, 1633, 1803
TseFI	GTSAC	3 cut(s)	335, 1813, 1825
Tsp45I	GTSAC	3 cut(s)	335, 1813, 1825
TspGWI	ACGGA	1 cut(s)	1602
TspRI	CASTG	4 cut(s)	478, 1105, 1633, 1803
Tth111I	GACNNNGTC	1 cut(s)	2674
Van91I	CCANNNNNTGG	2 cut(s)	1256, 2657
VpaK11BI	GGWCC	3 cut(s)	90, 872, 2661
VspI	ATTAAT	1 cut(s)	2424
XapI	RAATTY	3 cut(s)	483, 1594, 2725
XbaI	TCTAGA	1 cut(s)	487
XceI	RCATGY	3 cut(s)	456, 2266, 2456
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1005
XmiI	GTMKAC	2 cut(s)	883, 2259
XmnI	GAANNNNTTC	3 cut(s)	1016, 2082, 2366
XspI	CTAG	6 cut(s)	488, 596, 950, 1664, 1962, 2490
Zsp2I	ATGCAT	2 cut(s)	784, 2548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.