MD05G1082600.v1.1

NYN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013466
Physical Location & Seq
Forward (+)
17265440 .. 17268429
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1082600.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGCTTTCCCATATACATCAACATCAATAGTGCCTTCAGAACTGTCAGAGCAAAGAGGAGCAAATATATCAGATTCAAACACAAACATGTTTCAAGCTCCAACAAACCAGCCCAGCCGAGGCTCCTTTGATGGTCCTGTGGCTATCTTTTGGGATATTGAGAACTGCCCTGTCCCTAGTGATGTCCGTCCAGAAGATGTAGCTGGTAATATCAGAATGGCTTTACAGGTGCATCCCATAATTAAAGGAGCAGTTATGACGTTTTCTGCTTATGGGGACTTTAATGGCTTCCCTAGACGACTCCGCGAGGGATGCCAGAGAACTGGTGTTAGACTAATTGATGTTCCAAATGGAAGAAAGGATGCTGCTGACAAAGCTATCCTGGTTGATATGTTCCTGTTTGCCCTTGACAATCCTCCACCTTCTTCCATCATGCTCATCTCCGGGGATGTTGATTTTGCTCCAGCACTTCATATACTTGGGCAACGTGGATATATTGTTATCCTGGTAATCCCATCTGGAGTTGGTGTTTCATCTGCTCTCAGCAATGCTGGTAAGTTCGTGTGGGACTGGCCTAGTGTGGCTCGTGGAGACGGCTTTGTGCCTTCTACTAAAGTTTTAATGCATCCTCGTGGGGGTCACACTGATATTTCTGGGTATCTTATGGGTTGCCATATCAATGATAATGTGGATATCCAAAATGAAGAAGAAGCTATCTTGTATAAGGGAATATCACAAAGCTATTACAACTCGAGAGATTTTTCAATAGTCTCACAATCACTATCTGAATTCAACAGTAGTTCCGTAATGGTGCCTTGCTGTCCTGCTGCTTCAAGGTCACACAGTCTCCCGTCTGGTCTCAATGAAGTTTCTGCTGGGCCTACAACATCTGGTGATCAAAATGAATCTACTTGGTGGGTCCAGCCAGGGGACCTAAATGGTCTGAAGGGACAGCTTGTAAGACTGCTTGAGCTATCGGGAGGTTGCATGCCTCTTATGAAAGTTCCAACCGAGTATCAGAAAGTTTTTGGAAGGCCTCTTTATGTATCAGAGTATGGTGCATTCAAGCTCGTAAATCTGTTCAGGAAGTTGGGTGACACAATGGCTGTAGAGGGAAAAGGGAACAAGAGATTTGTCTACCTCCGGAACTGTAAAACGGGCACTAGTGCACCACCTTTGGTTTTGTTAAAGAGGGATAACAAGAAAGGGAAGGGGACTCAAGAAGAGTGCATGGATATTACTACAGGCAATGGCTCATCAGATGAGTTCTCAGAGGAGGAAAGAGTTGTTGTAGAAGAATACGATGAGACGAGCCAAGGAAAGACCAATGTGAGAACAGTAGGTGAATGTGGAATTGATGATCCTAGTCTGGAGAATTTCAAATATGAGCTGCAAGAGATTCTTGTAAGCTACTCTTGTCGGATTTTCTTGGGTTGCTTTGAGGAGGTCTACCAGCAGAGGTATAAAAAGACACTGGACTATCGGAAGTTTGGAGTTAATCAGCTAGAGCAACTGTTTGAGAAGGTGACAGATGTGGTGGTGTTGCTTGAAGAACCAGTTGGCAAGAGGAAGTTCCTGGCTGCAAGTGGTGGCTATAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN OST-HTH

Protein Analysis

535

Amino Acids

58.81

Weight (kDa)

5.36

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NYN PF01936 47 - 184 1.2e-26 NYN domain
OST-HTH PF12872 312 - 379 1.6e-09 OST-HTH/LOTUS domain
OST-HTH PF12872 456 - 520 8.2e-07 OST-HTH/LOTUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013466)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15560 AT2G15560
fragaria_vesca FvH4_2g05850 FvH4_2g05850
malus_domestica MD05G1082600.v1.1
prunus_persica Prupe.8G127000_v2.0.a1 Prupe.8G127000_v2.0.a1 Prupe.8G127000_v2.0.a1
pyrus_communis pycom05g07760
rosa_chinensis RchiOBHm_Chr6g0257731
rosa_laevigata RLG00000014719
rosa_multiflora Rmu_sc0011315.1_g000007
rosa_roxburghii Rroxscaffold_7G00208910
rosa_samantha Rh6AG085400 Rh6BG079700 Rh6CG074700 Rh6DG071600
rosa_wichuraiana Rw6G007670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 811
AccI GTMKAC 2 cut(s) 1137, 1449
AccII CGCG 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 1 cut(s) 304
AclWI GGATC 1 cut(s) 1355
AcsI RAATTY 2 cut(s) 788, 1375
AcuI CTGAAG 2 cut(s) 21, 965
AfiI CCNNNNNNNGG 3 cut(s) 119, 635, 928
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 8 cut(s) 78, 95, 765, 793, 834, 1066, 1381, 1550
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 4 cut(s) 381, 504, 925, 1575
AjuI GAANNNNNNNTTGG 2 cut(s) 1542, 1574
Alw21I GWGCWC 1 cut(s) 1171
Alw26I GTCTC 5 cut(s) 585, 775, 851, 863, 1301
Alw44I GTGCAC 1 cut(s) 1167
AlwI GGATC 1 cut(s) 1355
Ama87I CYCGRG 1 cut(s) 751
Aor13HI TCCGGA 1 cut(s) 1143
AoxI GGCC 3 cut(s) 572, 878, 1034
ApaLI GTGCAC 1 cut(s) 1167
ApeKI GCWGC 4 cut(s) 365, 827, 1390, 1580
ApoI RAATTY 2 cut(s) 788, 1375
AspS9I GGNCC 4 cut(s) 134, 878, 919, 931
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 4 cut(s) 905, 1106, 1355, 1537
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 3 cut(s) 134, 919, 931
BaeGI GKGCMC 2 cut(s) 1163, 1171
BanI GGYRCC 1 cut(s) 811
BauI CACGAG 2 cut(s) 585, 630
Bbv12I GWGCWC 1 cut(s) 1171
BbvI GCAGC 4 cut(s) 352, 814, 1377, 1567
BccI CCATC 3 cut(s) 125, 437, 523
BceAI ACGGC 1 cut(s) 610
BciT130I CCWGG 4 cut(s) 383, 506, 927, 1577
BclI TGATCA 1 cut(s) 895
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 5 cut(s) 585, 775, 851, 863, 1301
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 6 cut(s) 177, 294, 576, 1164, 1365, 1505
BfmI CTRYAG 2 cut(s) 1107, 1242
BisI GCNGC 4 cut(s) 366, 828, 1391, 1581
BlsI GCNGC 4 cut(s) 367, 829, 1392, 1582
Bme1390I CCNGG 5 cut(s) 383, 445, 506, 927, 1577
Bme18I GGWCC 3 cut(s) 134, 919, 931
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 4 cut(s) 134, 878, 919, 931
BmiI GGNNCC 4 cut(s) 124, 813, 920, 932
BmrFI CCNGG 5 cut(s) 383, 445, 506, 927, 1577
BmsI GCATC 4 cut(s) 241, 302, 352, 634
BpmI CTGGAG 3 cut(s) 447, 540, 1391
BpuEI CTTGAG 2 cut(s) 989, 1203
BpuMI CCSGG 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 863
BsaJI CCNNGG 4 cut(s) 118, 444, 926, 1315
BsaWI WCCGGW 1 cut(s) 1143
BsaXI ACNNNNNCTCC 4 cut(s) 513, 543, 831, 861
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 635, 928
Bse1I ACTGG 4 cut(s) 328, 575, 1479, 1556
Bse3DI GCAATG 2 cut(s) 553, 1255
BseAI TCCGGA 1 cut(s) 1143
BseBI CCWGG 4 cut(s) 383, 506, 927, 1577
BseDI CCNNGG 4 cut(s) 118, 444, 926, 1315
BseGI GGATG 5 cut(s) 232, 317, 367, 454, 625
BseLI CCNNNNNNNGG 3 cut(s) 119, 635, 928
BseMI GCAATG 2 cut(s) 553, 1255
BseMII CTCAG 2 cut(s) 556, 1284
BseNI ACTGG 4 cut(s) 328, 575, 1479, 1556
BseRI GAGGAG 3 cut(s) 72, 1289, 1457
BseSI GKGCMC 2 cut(s) 1163, 1171
BseXI GCAGC 4 cut(s) 352, 814, 1377, 1567
BseYI CCCAGC 2 cut(s) 113, 875
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 574, 880, 1036
BshNI GGYRCC 1 cut(s) 811
BsiHKAI GWGCWC 1 cut(s) 1171
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 2 cut(s) 444, 1144
BslFI GGGAC 6 cut(s) 158, 290, 581, 944, 963, 1228
BslI CCNNNNNNNGG 3 cut(s) 119, 635, 928
BsmAI GTCTC 5 cut(s) 585, 775, 851, 863, 1301
BsmBI CGTCTC 2 cut(s) 585, 1301
BsmFI GGGAC 6 cut(s) 158, 290, 581, 944, 963, 1228
BsmI GAATGC 1 cut(s) 1061
BsnI GGCC 3 cut(s) 574, 880, 1036
Bso31I GGTCTC 1 cut(s) 863
BsoBI CYCGRG 1 cut(s) 751
Bsp1286I GDGCHC 2 cut(s) 1163, 1171
Bsp13I TCCGGA 1 cut(s) 1143
Bsp143I GATC 2 cut(s) 895, 1360
BspACI CCGC 1 cut(s) 304
BspANI GGCC 3 cut(s) 574, 880, 1036
BspCNI CTCAG 2 cut(s) 555, 1283
BspEI TCCGGA 1 cut(s) 1143
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 4 cut(s) 124, 813, 920, 932
BspPI GGATC 1 cut(s) 1355
BspT107I GGYRCC 1 cut(s) 811
BspTNI GGTCTC 1 cut(s) 863
BsrDI GCAATG 2 cut(s) 553, 1255
BsrI ACTGG 4 cut(s) 328, 575, 1479, 1556
BssECI CCNNGG 4 cut(s) 118, 444, 926, 1315
BssMI GATC 2 cut(s) 895, 1360
BssSI CACGAG 2 cut(s) 585, 630
BssT1I CCWWGG 1 cut(s) 1315
Bst2BI CACGAG 2 cut(s) 585, 630
Bst2UI CCWGG 4 cut(s) 383, 506, 927, 1577
Bst4CI ACNGT 6 cut(s) 45, 797, 845, 1151, 1339, 1515
Bst6I CTCTTC 1 cut(s) 1218
BstC8I GCNNGC 1 cut(s) 989
BstDEI CTNAG 2 cut(s) 542, 1270
BstF5I GGATG 5 cut(s) 232, 317, 367, 454, 625
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 2 cut(s) 898, 1363
BstMAI GTCTC 5 cut(s) 585, 775, 851, 863, 1301
BstMBI GATC 2 cut(s) 895, 1360
BstMWI GCNNNNNNNGC 2 cut(s) 312, 374
BstNI CCWGG 4 cut(s) 383, 506, 927, 1577
BstNSI RCATGY 2 cut(s) 91, 991
BstSCI CCNGG 5 cut(s) 381, 443, 504, 925, 1575
BstSFI CTRYAG 2 cut(s) 1107, 1242
BstSLI GKGCMC 2 cut(s) 1163, 1171
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 4 cut(s) 352, 814, 1377, 1567
BstXI CCANNNNNNTGG 1 cut(s) 323
BsuRI GGCC 3 cut(s) 574, 880, 1036
BtsCI GGATG 5 cut(s) 232, 317, 367, 454, 625
BtsIMutI CAGTG 2 cut(s) 642, 1472
Cac8I GCNNGC 1 cut(s) 989
Cfr13I GGNCC 4 cut(s) 134, 878, 919, 931
CviAII CATG 4 cut(s) 88, 433, 988, 1231
DdeI CTNAG 2 cut(s) 542, 1270
DpnI GATC 2 cut(s) 897, 1362
DpnII GATC 2 cut(s) 895, 1360
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
Eco130I CCWWGG 1 cut(s) 1315
Eco147I AGGCCT 1 cut(s) 1036
Eco31I GGTCTC 1 cut(s) 863
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 3 cut(s) 134, 919, 931
Eco57I CTGAAG 2 cut(s) 21, 965
Eco88I CYCGRG 1 cut(s) 751
EcoO109I RGGNCCY 1 cut(s) 931
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 4 cut(s) 381, 504, 925, 1575
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 1315
EcoT22I ATGCAT 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 1315
Esp3I CGTCTC 2 cut(s) 585, 1301
FaeI CATG 4 cut(s) 91, 436, 991, 1234
FalI AAGNNNNNCTT 4 cut(s) 1386, 1418, 1399, 1431
FaqI GGGAC 6 cut(s) 158, 290, 581, 944, 963, 1228
FatI CATG 4 cut(s) 87, 432, 987, 1230
FbaI TGATCA 1 cut(s) 895
FblI GTMKAC 2 cut(s) 1137, 1449
Fnu4HI GCNGC 4 cut(s) 366, 828, 1391, 1581
FokI GGATG 5 cut(s) 219, 324, 374, 461, 612
Fsp4HI GCNGC 4 cut(s) 366, 828, 1391, 1581
FspBI CTAG 6 cut(s) 177, 294, 576, 1164, 1365, 1505
GluI GCNGC 4 cut(s) 366, 828, 1391, 1581
GsaI CCCAGC 2 cut(s) 117, 879
GsuI CTGGAG 3 cut(s) 447, 540, 1391
HaeIII GGCC 3 cut(s) 574, 880, 1036
HapII CCGG 2 cut(s) 444, 1144
Hin1II CATG 4 cut(s) 91, 436, 991, 1234
HinfI GANTC 5 cut(s) 74, 300, 905, 1216, 1399
HpaII CCGG 2 cut(s) 444, 1144
HphI GGTGA 4 cut(s) 905, 1106, 1355, 1537
Hpy166II GTNNAC 3 cut(s) 1138, 1169, 1450
Hpy188III TCNNGA 8 cut(s) 191, 519, 753, 978, 1084, 1144, 1220, 1370
Hpy8I GTNNAC 3 cut(s) 1138, 1169, 1450
HpyAV CCTTC 7 cut(s) 45, 432, 615, 940, 1026, 1204, 1516
HpyCH4III ACNGT 6 cut(s) 45, 797, 845, 1151, 1339, 1515
HpyCH4IV ACGT 2 cut(s) 260, 487
HpyCH4V TGCA 8 cut(s) 232, 625, 987, 1061, 1169, 1230, 1393, 1583
HpyF10VI GCNNNNNNNGC 2 cut(s) 312, 374
HpyF3I CTNAG 2 cut(s) 542, 1270
HpySE526I ACGT 2 cut(s) 260, 487
Hsp92II CATG 4 cut(s) 91, 436, 991, 1234
Kpn2I TCCGGA 1 cut(s) 1143
Ksp22I TGATCA 1 cut(s) 895
Kzo9I GATC 2 cut(s) 895, 1360
LmnI GCTCC 5 cut(s) 59, 103, 128, 248, 466
Lsp1109I GCAGC 4 cut(s) 352, 814, 1377, 1567
LweI GCATC 4 cut(s) 241, 302, 352, 634
MaeI CTAG 6 cut(s) 177, 294, 576, 1164, 1365, 1505
MaeII ACGT 2 cut(s) 260, 487
MaeIII GTNAC 4 cut(s) 638, 837, 1094, 1525
MalI GATC 2 cut(s) 897, 1362
MboI GATC 2 cut(s) 895, 1360
MboII GAAGA 8 cut(s) 206, 366, 417, 716, 719, 1235, 1307, 1562
MhlI GDGCHC 2 cut(s) 1163, 1171
MluCI AATT 6 cut(s) 240, 336, 788, 1353, 1375, 1600
MlyI GAGTC 2 cut(s) 294, 1210
MmeI TCCRAC 3 cut(s) 125, 1031, 1400
Mph1103I ATGCAT 1 cut(s) 627
MroI TCCGGA 1 cut(s) 1143
MseI TTAA 6 cut(s) 243, 282, 620, 1187, 1497, 1603
MslI CAYNNNNRTG 3 cut(s) 86, 630, 678
MspI CCGG 2 cut(s) 444, 1144
MspR9I CCNGG 5 cut(s) 383, 445, 506, 927, 1577
Mva1269I GAATGC 1 cut(s) 1061
MvaI CCWGG 4 cut(s) 383, 506, 927, 1577
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 2 cut(s) 312, 374
NciI CCSGG 1 cut(s) 445
NdeII GATC 2 cut(s) 895, 1360
NlaIII CATG 4 cut(s) 91, 436, 991, 1234
NlaIV GGNNCC 4 cut(s) 124, 813, 920, 932
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 4 cut(s) 638, 837, 1094, 1525
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 2 cut(s) 91, 991
PaeI GCATGC 1 cut(s) 991
PaeR7I CTCGAG 1 cut(s) 751
PceI AGGCCT 1 cut(s) 1036
PciI ACATGT 1 cut(s) 87
PctI GAATGC 1 cut(s) 1061
PfeI GAWTC 3 cut(s) 74, 905, 1399
PkrI GCNGC 4 cut(s) 367, 829, 1392, 1582
PleI GAGTC 2 cut(s) 294, 1210
PpsI GAGTC 2 cut(s) 294, 1210
PpuMI RGGWCCY 1 cut(s) 931
PscI ACATGT 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 4 cut(s) 381, 504, 925, 1575
PspFI CCCAGC 2 cut(s) 113, 875
PspGI CCWGG 4 cut(s) 381, 504, 925, 1575
PspN4I GGNNCC 4 cut(s) 124, 813, 920, 932
PspPI GGNCC 4 cut(s) 134, 878, 919, 931
PspPPI RGGWCCY 1 cut(s) 931
RseI CAYNNNNRTG 3 cut(s) 86, 630, 678
SaqAI TTAA 6 cut(s) 243, 282, 620, 1187, 1497, 1603
SatI GCNGC 4 cut(s) 366, 828, 1391, 1581
Sau3AI GATC 2 cut(s) 895, 1360
Sau96I GGNCC 4 cut(s) 134, 878, 919, 931
SchI GAGTC 2 cut(s) 294, 1210
ScrFI CCNGG 5 cut(s) 383, 445, 506, 927, 1577
SduI GDGCHC 2 cut(s) 1163, 1171
SfaNI GCATC 4 cut(s) 241, 302, 352, 634
SfcI CTRYAG 2 cut(s) 1107, 1242
Sfr274I CTCGAG 1 cut(s) 751
SinI GGWCC 3 cut(s) 134, 919, 931
SlaI CTCGAG 1 cut(s) 751
SmiMI CAYNNNNRTG 3 cut(s) 86, 630, 678
SmlI CTYRAG 3 cut(s) 751, 968, 1218
SmoI CTYRAG 3 cut(s) 751, 968, 1218
SpeI ACTAGT 1 cut(s) 1163
SphI GCATGC 1 cut(s) 991
Sse9I AATT 6 cut(s) 240, 336, 788, 1353, 1375, 1600
SseBI AGGCCT 1 cut(s) 1036
SsiI CCGC 1 cut(s) 304
SspMI CTAG 6 cut(s) 177, 294, 576, 1164, 1365, 1505
StuI AGGCCT 1 cut(s) 1036
StyD4I CCNGG 5 cut(s) 381, 443, 504, 925, 1575
StyI CCWWGG 1 cut(s) 1315
TaaI ACNGT 6 cut(s) 45, 797, 845, 1151, 1339, 1515
TaiI ACGT 2 cut(s) 263, 490
TaqI TCGA 1 cut(s) 752
TasI AATT 6 cut(s) 240, 336, 788, 1353, 1375, 1600
TfiI GAWTC 3 cut(s) 74, 905, 1399
Tru1I TTAA 6 cut(s) 243, 282, 620, 1187, 1497, 1603
Tru9I TTAA 6 cut(s) 243, 282, 620, 1187, 1497, 1603
TscAI CASTG 2 cut(s) 649, 1479
TseFI GTSAC 4 cut(s) 638, 837, 1094, 1525
TseI GCWGC 4 cut(s) 365, 827, 1390, 1580
Tsp45I GTSAC 4 cut(s) 638, 837, 1094, 1525
TspDTI ATGAA 6 cut(s) 461, 522, 717, 879, 918, 1013
TspGWI ACGGA 2 cut(s) 176, 793
TspRI CASTG 2 cut(s) 649, 1479
VneI GTGCAC 1 cut(s) 1167
VpaK11BI GGWCC 3 cut(s) 134, 919, 931
XapI RAATTY 2 cut(s) 788, 1375
XceI RCATGY 2 cut(s) 91, 991
XhoI CTCGAG 1 cut(s) 751
XmiI GTMKAC 2 cut(s) 1137, 1449
XspI CTAG 6 cut(s) 177, 294, 576, 1164, 1365, 1505
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.