Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0019781

Physical Location & Seq

Genomic Coordinates Forward (+)

25451591 .. 25453329

Gene Structure 1739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1130100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 696 bp

ATGGTGAAGAGAGGCTCCTGGCGTATCCATGGAACTTTGTGGTGGGACCATGTGAGCAATACTTCTGAAATACGCGGGTTCAAGATAATCTCAGAACAGGGTATTGCATCTGGAATAAGGCGGATAGAAGCTGTGGCAGGTGATGCCTTTATTGAATATGTCAATGCCAGAGATTATTACATGAAACAACTATGTTCCACTCTCAAAGTGAAAGCTGAAGAAGTAACAAACAGGGCAGATAATCTGCTAGAGGAGTTACGGATGAAAAGAAACGAAGCTTCTACCTTACGAGAAAAAGCAGCAGTTTACAAAGGATCAATCACGGCAAGCAAAGCGATTTCTGTGGGAACTTCACAAAAAGTCAGGGTACTAGTTGAATCCATGGAAGACACTGATGCCGATTCCCTAAAAAAGGCAGCCGAATATCTGATTGAGACGCTAGAAGATCCGGCAGCTGTAATTCTGGGCTCTTGTCCAGGTGACGAGAAGTTAAGTCTGGTGGCTGCATTCACCCCAGGGGTTGTCAAGTTTGGAATACAGGCCGGGAAGTTCATAGGGCCCATAGCTAATTTGTGCGGTGGTGGAGGGGGTGGAAGACCCAACTTTGCTCAGGCAGGAGGGCGGAAGCCTGACAATTTGTCCAATGCACTTGAAACAGCTCGGTCGGAGATCATCTCAGTTGAAAAGTCAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000049 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016020 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0031406 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

232

Amino Acids

24.92

Weight (kDa)

8.36

Isoelectric Point (pI)

39.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_SAD	PF07973	17 - 43	1.3e-07	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	121 - 224	2.1e-15	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019781)

Species	Orthologous Gene IDs
malus_domestica	MD05G1130100.v1.1 MD05G1130200.v1.1
rosa_multiflora	Rmu_sc0000258.1_g000017
rosa_roxburghii	Rroxscaffold_7G00189760 Rroxscaffold_7G00190040
rosa_samantha	Rh6BG231000 Rh7DG059100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	128
Acc36I	ACCTGC	1 cut(s)	128
AccII	CGCG	1 cut(s)	75
AciI	CCGC	4 cut(s)	75, 121, 576, 622
AclWI	GGATC	2 cut(s)	322, 440
AcuI	CTGAAG	1 cut(s)	237
AfaI	GTAC	1 cut(s)	369
AfiI	CCNNNNNNNGG	1 cut(s)	412
AgsI	TTSAA	5 cut(s)	82, 155, 377, 653, 683
AhdI	GACNNNNNGTC	1 cut(s)	637
AhlI	ACTAGT	1 cut(s)	370
AjnI	CCWGG	3 cut(s)	17, 475, 514
AluBI	AGCT	6 cut(s)	131, 215, 278, 455, 566, 659
AluI	AGCT	6 cut(s)	131, 215, 278, 455, 566, 659
Alw26I	GTCTC	1 cut(s)	428
AlwI	GGATC	2 cut(s)	322, 440
AoxI	GGCC	2 cut(s)	540, 557
ApaI	GGGCCC	1 cut(s)	561
ApeKI	GCWGC	4 cut(s)	299, 416, 452, 503
AspS9I	GGNCC	3 cut(s)	46, 557, 558
AsuC2I	CCSGG	1 cut(s)	544
AsuHPI	GGTGA	4 cut(s)	16, 152, 491, 502
AvaII	GGWCC	1 cut(s)	46
BaeGI	GKGCMC	1 cut(s)	561
BanII	GRGCYC	2 cut(s)	470, 561
BbsI	GAAGAC	2 cut(s)	393, 601
BbvI	GCAGC	4 cut(s)	311, 428, 464, 490
BceAI	ACGGC	1 cut(s)	339
BciT130I	CCWGG	3 cut(s)	19, 477, 516
BciVI	GTATCC	1 cut(s)	35
BcnI	CCSGG	1 cut(s)	544
BcoDI	GTCTC	1 cut(s)	428
BcuI	ACTAGT	1 cut(s)	370
BfaI	CTAG	3 cut(s)	248, 371, 440
BfuAI	ACCTGC	1 cut(s)	128
BfuI	GTATCC	1 cut(s)	35
BisI	GCNGC	4 cut(s)	300, 417, 453, 504
BlsI	GCNGC	4 cut(s)	301, 418, 454, 505
Bme1390I	CCNGG	4 cut(s)	19, 477, 516, 544
Bme18I	GGWCC	1 cut(s)	46
BmeRI	GACNNNNNGTC	1 cut(s)	637
BmgT120I	GGNCC	3 cut(s)	46, 557, 558
BmiI	GGNNCC	3 cut(s)	16, 47, 559
BmrFI	CCNGG	4 cut(s)	19, 477, 516, 544
BmsI	GCATC	3 cut(s)	116, 133, 385
BpiI	GAAGAC	2 cut(s)	393, 601
BplI	GAGNNNNNCTC	2 cut(s)	659, 691
Bpu10I	CCTNAGC	1 cut(s)	609
BpuMI	CCSGG	1 cut(s)	544
BsaJI	CCNNGG	4 cut(s)	28, 381, 514, 515
Bsc4I	CCNNNNNNNGG	1 cut(s)	412
BseBI	CCWGG	3 cut(s)	19, 477, 516
BseDI	CCNNGG	4 cut(s)	28, 381, 514, 515
BseGI	GGATG	1 cut(s)	267
BseLI	CCNNNNNNNGG	1 cut(s)	412
BseMII	CTCAG	3 cut(s)	105, 623, 690
BseRI	GAGGAG	1 cut(s)	266
BseSI	GKGCMC	1 cut(s)	561
BseXI	GCAGC	4 cut(s)	311, 428, 464, 490
Bsh1236I	CGCG	1 cut(s)	75
Bsh1285I	CGRYCG	1 cut(s)	665
BshFI	GGCC	2 cut(s)	542, 559
BsiEI	CGRYCG	1 cut(s)	665
BsiSI	CCGG	2 cut(s)	449, 543
BslFI	GGGAC	1 cut(s)	59
BslI	CCNNNNNNNGG	1 cut(s)	412
BsmAI	GTCTC	1 cut(s)	428
BsmBI	CGTCTC	1 cut(s)	428
BsmFI	GGGAC	1 cut(s)	59
BsmI	GAATGC	1 cut(s)	506
BsnI	GGCC	2 cut(s)	542, 559
Bsp120I	GGGCCC	1 cut(s)	557
Bsp1286I	GDGCHC	2 cut(s)	470, 561
Bsp143I	GATC	3 cut(s)	314, 445, 669
Bsp19I	CCATGG	2 cut(s)	28, 381
BspACI	CCGC	4 cut(s)	75, 121, 576, 622
BspANI	GGCC	2 cut(s)	542, 559
BspCNI	CTCAG	3 cut(s)	104, 622, 689
BspFNI	CGCG	1 cut(s)	75
BspLI	GGNNCC	3 cut(s)	16, 47, 559
BspMI	ACCTGC	1 cut(s)	128
BspPI	GGATC	2 cut(s)	322, 440
BssECI	CCNNGG	4 cut(s)	28, 381, 514, 515
BssMI	GATC	3 cut(s)	314, 445, 669
BssT1I	CCWWGG	2 cut(s)	28, 381
Bst2UI	CCWGG	3 cut(s)	19, 477, 516
Bst6I	CTCTTC	1 cut(s)	2
BstAPI	GCANNNNNTGC	1 cut(s)	143
BstC8I	GCNNGC	1 cut(s)	328
BstDEI	CTNAG	3 cut(s)	91, 609, 676
BstDSI	CCRYGG	2 cut(s)	28, 381
BstENI	CCTNNNNNAGG	1 cut(s)	410
BstF5I	GGATG	1 cut(s)	267
BstFNI	CGCG	1 cut(s)	75
BstKTI	GATC	3 cut(s)	317, 448, 672
BstMAI	GTCTC	1 cut(s)	428
BstMBI	GATC	3 cut(s)	314, 445, 669
BstMCI	CGRYCG	1 cut(s)	665
BstMWI	GCNNNNNNNGC	2 cut(s)	143, 332
BstNI	CCWGG	3 cut(s)	19, 477, 516
BstSCI	CCNGG	4 cut(s)	17, 475, 514, 542
BstSLI	GKGCMC	1 cut(s)	561
BstUI	CGCG	1 cut(s)	75
BstV1I	GCAGC	4 cut(s)	311, 428, 464, 490
BstV2I	GAAGAC	2 cut(s)	393, 601
BstX2I	RGATCY	1 cut(s)	445
BstYI	RGATCY	1 cut(s)	445
BsuI	GTATCC	1 cut(s)	35
BsuRI	GGCC	2 cut(s)	542, 559
BtgI	CCRYGG	2 cut(s)	28, 381
BtsCI	GGATG	1 cut(s)	267
BtsIMutI	CAGTG	1 cut(s)	390
BveI	ACCTGC	1 cut(s)	128
Cac8I	GCNNGC	1 cut(s)	328
Cfr13I	GGNCC	3 cut(s)	46, 557, 558
CseI	GACGC	1 cut(s)	445
Csp6I	GTAC	1 cut(s)	368
CviAII	CATG	4 cut(s)	29, 50, 181, 382
CviQI	GTAC	1 cut(s)	368
DdeI	CTNAG	3 cut(s)	91, 609, 676
DpnI	GATC	3 cut(s)	316, 447, 671
DpnII	GATC	3 cut(s)	314, 445, 669
DriI	GACNNNNNGTC	1 cut(s)	637
Eam1104I	CTCTTC	1 cut(s)	2
Eam1105I	GACNNNNNGTC	1 cut(s)	637
EarI	CTCTTC	1 cut(s)	2
EciI	GGCGGA	2 cut(s)	136, 637
Eco130I	CCWWGG	2 cut(s)	28, 381
Eco24I	GRGCYC	2 cut(s)	470, 561
Eco47I	GGWCC	1 cut(s)	46
Eco57I	CTGAAG	1 cut(s)	237
EcoNI	CCTNNNNNAGG	1 cut(s)	410
EcoO109I	RGGNCCY	1 cut(s)	557
EcoRII	CCWGG	3 cut(s)	17, 475, 514
EcoT14I	CCWWGG	2 cut(s)	28, 381
EcoT38I	GRGCYC	2 cut(s)	470, 561
ErhI	CCWWGG	2 cut(s)	28, 381
Esp3I	CGTCTC	1 cut(s)	428
FaeI	CATG	4 cut(s)	32, 53, 184, 385
FaiI	YATR	8 cut(s)	30, 51, 159, 182, 193, 383, 554, 563
FaqI	GGGAC	1 cut(s)	59
FatI	CATG	4 cut(s)	28, 49, 180, 381
FauI	CCCGC	1 cut(s)	68
Fnu4HI	GCNGC	4 cut(s)	300, 417, 453, 504
FokI	GGATG	1 cut(s)	274
FriOI	GRGCYC	2 cut(s)	470, 561
Fsp4HI	GCNGC	4 cut(s)	300, 417, 453, 504
FspBI	CTAG	3 cut(s)	248, 371, 440
GluI	GCNGC	4 cut(s)	300, 417, 453, 504
HaeIII	GGCC	2 cut(s)	542, 559
HapII	CCGG	2 cut(s)	449, 543
HgaI	GACGC	1 cut(s)	445
Hin1II	CATG	4 cut(s)	32, 53, 184, 385
HindIII	AAGCTT	1 cut(s)	276
HinfI	GANTC	2 cut(s)	377, 401
HpaII	CCGG	2 cut(s)	449, 543
HphI	GGTGA	4 cut(s)	16, 152, 491, 502
Hpy166II	GTNNAC	1 cut(s)	307
Hpy188I	TCNGA	4 cut(s)	67, 94, 429, 667
Hpy188III	TCNNGA	2 cut(s)	82, 111
Hpy8I	GTNNAC	1 cut(s)	307
HpyCH4V	TGCA	3 cut(s)	107, 506, 647
HpyF10VI	GCNNNNNNNGC	2 cut(s)	143, 332
HpyF3I	CTNAG	3 cut(s)	91, 609, 676
Hsp92II	CATG	4 cut(s)	32, 53, 184, 385
Kzo9I	GATC	3 cut(s)	314, 445, 669
LmnI	GCTCC	1 cut(s)	20
Lsp1109I	GCAGC	4 cut(s)	311, 428, 464, 490
LweI	GCATC	3 cut(s)	116, 133, 385
MaeI	CTAG	3 cut(s)	248, 371, 440
MaeIII	GTNAC	3 cut(s)	223, 255, 479
MalI	GATC	3 cut(s)	316, 447, 671
MboI	GATC	3 cut(s)	314, 445, 669
MboII	GAAGA	5 cut(s)	19, 230, 398, 455, 606
MflI	RGATCY	1 cut(s)	445
MhlI	GDGCHC	2 cut(s)	470, 561
MluCI	AATT	3 cut(s)	459, 568, 634
MmeI	TCCRAC	1 cut(s)	645
MnlI	CCTC	4 cut(s)	5, 244, 578, 611
MseI	TTAA	2 cut(s)	491, 694
MspA1I	CMGCKG	1 cut(s)	455
MspI	CCGG	2 cut(s)	449, 543
MspR9I	CCNGG	4 cut(s)	19, 477, 516, 544
Mva1269I	GAATGC	1 cut(s)	506
MvaI	CCWGG	3 cut(s)	19, 477, 516
MvnI	CGCG	1 cut(s)	75
MwoI	GCNNNNNNNGC	2 cut(s)	143, 332
NciI	CCSGG	1 cut(s)	544
NcoI	CCATGG	2 cut(s)	28, 381
NdeII	GATC	3 cut(s)	314, 445, 669
NlaIII	CATG	4 cut(s)	32, 53, 184, 385
NlaIV	GGNNCC	3 cut(s)	16, 47, 559
NmuCI	GTSAC	1 cut(s)	479
PaqCI	CACCTGC	1 cut(s)	128
PasI	CCCWGGG	1 cut(s)	515
PctI	GAATGC	1 cut(s)	506
PfeI	GAWTC	2 cut(s)	377, 401
PkrI	GCNGC	4 cut(s)	301, 418, 454, 505
Psp6I	CCWGG	3 cut(s)	17, 475, 514
PspGI	CCWGG	3 cut(s)	17, 475, 514
PspN4I	GGNNCC	3 cut(s)	16, 47, 559
PspOMI	GGGCCC	1 cut(s)	557
PspPI	GGNCC	3 cut(s)	46, 557, 558
PsuI	RGATCY	1 cut(s)	445
PvuII	CAGCTG	1 cut(s)	455
RsaI	GTAC	1 cut(s)	369
RsaNI	GTAC	1 cut(s)	368
SaqAI	TTAA	2 cut(s)	491, 694
SatI	GCNGC	4 cut(s)	300, 417, 453, 504
Sau3AI	GATC	3 cut(s)	314, 445, 669
Sau96I	GGNCC	3 cut(s)	46, 557, 558
ScrFI	CCNGG	4 cut(s)	19, 477, 516, 544
SduI	GDGCHC	2 cut(s)	470, 561
SetI	ASST	9 cut(s)	133, 142, 217, 280, 287, 457, 481, 568, 661
SfaNI	GCATC	3 cut(s)	116, 133, 385
SinI	GGWCC	1 cut(s)	46
SpeI	ACTAGT	1 cut(s)	370
Sse9I	AATT	3 cut(s)	459, 568, 634
SsiI	CCGC	4 cut(s)	75, 121, 576, 622
SspMI	CTAG	3 cut(s)	248, 371, 440
StyD4I	CCNGG	4 cut(s)	17, 475, 514, 542
StyI	CCWWGG	2 cut(s)	28, 381
TaqII	GACCGA	1 cut(s)	651
TasI	AATT	3 cut(s)	459, 568, 634
TfiI	GAWTC	2 cut(s)	377, 401
Tru1I	TTAA	2 cut(s)	491, 694
Tru9I	TTAA	2 cut(s)	491, 694
TscAI	CASTG	1 cut(s)	397
TseFI	GTSAC	1 cut(s)	479
TseI	GCWGC	4 cut(s)	299, 416, 452, 503
Tsp45I	GTSAC	1 cut(s)	479
TspDTI	ATGAA	3 cut(s)	197, 278, 541
TspGWI	ACGGA	1 cut(s)	274
TspRI	CASTG	1 cut(s)	397
VpaK11BI	GGWCC	1 cut(s)	46
XagI	CCTNNNNNAGG	1 cut(s)	410
XspI	CTAG	3 cut(s)	248, 371, 440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.