Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain
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| Species | Orthologous Gene IDs |
|---|---|
| malus_domestica | MD05G1130100.v1.1 MD05G1130200.v1.1 |
| rosa_multiflora | Rmu_sc0000258.1_g000017 |
| rosa_roxburghii | Rroxscaffold_7G00189760 Rroxscaffold_7G00190040 |
| rosa_samantha | Rh6BG231000 Rh7DG059100 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 143 |
| Acc36I | ACCTGC | 1 cut(s) | 143 |
| AcsI | RAATTY | 1 cut(s) | 171 |
| AjnI | CCWGG | 1 cut(s) | 52 |
| AoxI | GGCC | 2 cut(s) | 8, 95 |
| ApeKI | GCWGC | 1 cut(s) | 41 |
| ApoI | RAATTY | 1 cut(s) | 171 |
| AspS9I | GGNCC | 1 cut(s) | 95 |
| AsuHPI | GGTGA | 1 cut(s) | 40 |
| BbvI | GCAGC | 1 cut(s) | 28 |
| BciT130I | CCWGG | 1 cut(s) | 54 |
| BfuAI | ACCTGC | 1 cut(s) | 143 |
| BisI | GCNGC | 1 cut(s) | 42 |
| BlsI | GCNGC | 1 cut(s) | 43 |
| Bme1390I | CCNGG | 1 cut(s) | 54 |
| BmgT120I | GGNCC | 1 cut(s) | 95 |
| BmrFI | CCNGG | 1 cut(s) | 54 |
| Bpu10I | CCTNAGC | 1 cut(s) | 147 |
| BsaJI | CCNNGG | 3 cut(s) | 32, 52, 53 |
| Bse1I | ACTGG | 1 cut(s) | 64 |
| BseBI | CCWGG | 1 cut(s) | 54 |
| BseDI | CCNNGG | 3 cut(s) | 32, 52, 53 |
| BseGI | GGATG | 1 cut(s) | 165 |
| BseMII | CTCAG | 1 cut(s) | 161 |
| BseNI | ACTGG | 1 cut(s) | 64 |
| BseXI | GCAGC | 1 cut(s) | 28 |
| BshFI | GGCC | 2 cut(s) | 10, 97 |
| BsmI | GAATGC | 2 cut(s) | 44, 78 |
| BsnI | GGCC | 2 cut(s) | 10, 97 |
| BspANI | GGCC | 2 cut(s) | 10, 97 |
| BspCNI | CTCAG | 1 cut(s) | 160 |
| BspMI | ACCTGC | 1 cut(s) | 143 |
| BsrI | ACTGG | 1 cut(s) | 64 |
| BssECI | CCNNGG | 3 cut(s) | 32, 52, 53 |
| BssT1I | CCWWGG | 1 cut(s) | 32 |
| Bst2UI | CCWGG | 1 cut(s) | 54 |
| Bst4CI | ACNGT | 1 cut(s) | 200 |
| BstC8I | GCNNGC | 1 cut(s) | 78 |
| BstDEI | CTNAG | 3 cut(s) | 105, 147, 213 |
| BstF5I | GGATG | 1 cut(s) | 165 |
| BstNI | CCWGG | 1 cut(s) | 54 |
| BstNSI | RCATGY | 1 cut(s) | 80 |
| BstSCI | CCNGG | 1 cut(s) | 52 |
| BstV1I | GCAGC | 1 cut(s) | 28 |
| BsuRI | GGCC | 2 cut(s) | 10, 97 |
| BtsCI | GGATG | 1 cut(s) | 165 |
| BveI | ACCTGC | 1 cut(s) | 143 |
| Cac8I | GCNNGC | 1 cut(s) | 78 |
| Cfr13I | GGNCC | 1 cut(s) | 95 |
| CviAII | CATG | 1 cut(s) | 77 |
| CviJI | RGCY | 3 cut(s) | 10, 97, 136 |
| CviKI_1 | RGCY | 3 cut(s) | 10, 97, 136 |
| DdeI | CTNAG | 3 cut(s) | 105, 147, 213 |
| Eco130I | CCWWGG | 1 cut(s) | 32 |
| Eco147I | AGGCCT | 1 cut(s) | 10 |
| EcoO109I | RGGNCCY | 1 cut(s) | 95 |
| EcoRII | CCWGG | 1 cut(s) | 52 |
| EcoT14I | CCWWGG | 1 cut(s) | 32 |
| EcoT22I | ATGCAT | 1 cut(s) | 78 |
| ErhI | CCWWGG | 1 cut(s) | 32 |
| FaeI | CATG | 1 cut(s) | 80 |
| FaiI | YATR | 3 cut(s) | 28, 78, 92 |
| FatI | CATG | 1 cut(s) | 76 |
| Fnu4HI | GCNGC | 1 cut(s) | 42 |
| FokI | GGATG | 1 cut(s) | 172 |
| Fsp4HI | GCNGC | 1 cut(s) | 42 |
| GluI | GCNGC | 1 cut(s) | 42 |
| HaeIII | GGCC | 2 cut(s) | 10, 97 |
| Hin1II | CATG | 1 cut(s) | 80 |
| HphI | GGTGA | 1 cut(s) | 40 |
| HpyCH4III | ACNGT | 1 cut(s) | 200 |
| HpyCH4V | TGCA | 4 cut(s) | 44, 76, 80, 184 |
| HpyF3I | CTNAG | 3 cut(s) | 105, 147, 213 |
| Hsp92II | CATG | 1 cut(s) | 80 |
| LpnPI | CCDG | 7 cut(s) | 39, 66, 66, 77, 134, 138, 180 |
| Lsp1109I | GCAGC | 1 cut(s) | 28 |
| MluCI | AATT | 3 cut(s) | 17, 139, 171 |
| MmeI | TCCRAC | 1 cut(s) | 182 |
| MnlI | CCTC | 1 cut(s) | 116 |
| Mph1103I | ATGCAT | 1 cut(s) | 78 |
| MspR9I | CCNGG | 1 cut(s) | 54 |
| Mva1269I | GAATGC | 2 cut(s) | 44, 78 |
| MvaI | CCWGG | 1 cut(s) | 54 |
| NlaIII | CATG | 1 cut(s) | 80 |
| NsiI | ATGCAT | 1 cut(s) | 78 |
| NspI | RCATGY | 1 cut(s) | 80 |
| PaeI | GCATGC | 1 cut(s) | 80 |
| PaqCI | CACCTGC | 1 cut(s) | 143 |
| PasI | CCCWGGG | 1 cut(s) | 53 |
| PceI | AGGCCT | 1 cut(s) | 10 |
| PctI | GAATGC | 2 cut(s) | 44, 78 |
| PkrI | GCNGC | 1 cut(s) | 43 |
| Psp6I | CCWGG | 1 cut(s) | 52 |
| PspGI | CCWGG | 1 cut(s) | 52 |
| PspPI | GGNCC | 1 cut(s) | 95 |
| SatI | GCNGC | 1 cut(s) | 42 |
| Sau96I | GGNCC | 1 cut(s) | 95 |
| ScrFI | CCNGG | 1 cut(s) | 54 |
| SetI | ASST | 2 cut(s) | 157, 169 |
| SphI | GCATGC | 1 cut(s) | 80 |
| Sse9I | AATT | 3 cut(s) | 17, 139, 171 |
| SseBI | AGGCCT | 1 cut(s) | 10 |
| StuI | AGGCCT | 1 cut(s) | 10 |
| StyD4I | CCNGG | 1 cut(s) | 52 |
| StyI | CCWWGG | 1 cut(s) | 32 |
| TaaI | ACNGT | 1 cut(s) | 200 |
| TaqI | TCGA | 1 cut(s) | 188 |
| TasI | AATT | 3 cut(s) | 17, 139, 171 |
| TseI | GCWGC | 1 cut(s) | 41 |
| TspDTI | ATGAA | 2 cut(s) | 79, 176 |
| XapI | RAATTY | 1 cut(s) | 171 |
| XceI | RCATGY | 1 cut(s) | 80 |
| Zsp2I | ATGCAT | 1 cut(s) | 78 |