MD05G1166900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008838
Physical Location & Seq
Forward (+)
29702849 .. 29703307
459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1166900.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGTTGTGTTCCTTCTCAAACTCATCTTCTTCGCATTGAGCTTCGTATCGAACTTGGTTACGCGCTTGATCTTCTCTGCGACGGCTCATATTCTCGTGTTATTGATCCAGGGATTCAGAACACCAGGCCAAGCCATCCATGGCACATTGCAACTAGTCACCGAGGTTATCAAGTCATGTTCTGAGTACTTTGCGGGACTTGTAATGGAAGCAATAAGTGCTACAATCTCTACTTTCTTTGATTTTCTGAAAGGAAGTGTCACAGGATTTGCTGGCATTACCACTTCTGCGATTGTAGGTCTCGTGGAGAAAACAAAGACTTCTCTTGAATCGGTCGATGGGTTGCTCACTGATTATCCAGAGGTTTCGAAGGGGTTTTCTGAGATGGTTTATCGTATTGTGAATAATACGTGGAATGATTTTAAGGATGCGTTTGAATATGTAACAGAGAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.7

Weight (kDa)

5.06

Isoelectric Point (pI)

13.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 64
AciI CCGC 1 cut(s) 194
AclWI GGATC 1 cut(s) 100
AfaI GTAC 1 cut(s) 188
AgsI TTSAA 2 cut(s) 329, 437
AhlI ACTAGT 1 cut(s) 154
AjnI CCWGG 2 cut(s) 108, 124
AluBI AGCT 1 cut(s) 42
AluI AGCT 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 1 cut(s) 100
AoxI GGCC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 151
AsuII TTCGAA 1 cut(s) 368
BauI CACGAG 2 cut(s) 95, 302
BccI CCATC 3 cut(s) 143, 332, 379
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 2 cut(s) 110, 126
BcoDI GTCTC 1 cut(s) 305
BcuI ACTAGT 1 cut(s) 154
BfaI CTAG 1 cut(s) 155
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 110, 126
BmrFI CCNGG 2 cut(s) 110, 126
BmsI GCATC 1 cut(s) 418
Bpu14I TTCGAA 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 3 cut(s) 109, 139, 162
Bse3DI GCAATG 1 cut(s) 146
BseBI CCWGG 2 cut(s) 110, 126
BseDI CCNNGG 3 cut(s) 109, 139, 162
BseGI GGATG 2 cut(s) 135, 433
BseMI GCAATG 1 cut(s) 146
BseMII CTCAG 2 cut(s) 174, 372
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 1 cut(s) 129
Bso31I GGTCTC 1 cut(s) 305
Bsp119I TTCGAA 1 cut(s) 368
Bsp143I GATC 2 cut(s) 69, 105
Bsp19I CCATGG 1 cut(s) 139
BspACI CCGC 1 cut(s) 194
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 2 cut(s) 175, 373
BspFNI CGCG 1 cut(s) 64
BspPI GGATC 1 cut(s) 100
BspT104I TTCGAA 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 109, 139, 162
BssMI GATC 2 cut(s) 69, 105
BssSI CACGAG 2 cut(s) 95, 302
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 2 cut(s) 95, 302
Bst2UI CCWGG 2 cut(s) 110, 126
BstAPI GCANNNNNTGC 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 411
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 2 cut(s) 183, 381
BstDSI CCRYGG 1 cut(s) 139
BstF5I GGATG 2 cut(s) 135, 433
BstFNI CGCG 1 cut(s) 64
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 2 cut(s) 72, 108
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 2 cut(s) 69, 105
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNI CCWGG 2 cut(s) 110, 126
BstSCI CCNGG 2 cut(s) 108, 124
BstUI CGCG 1 cut(s) 64
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 139
BtsCI GGATG 2 cut(s) 135, 433
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 274
CfoI GCGC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 140, 177
CviJI RGCY 4 cut(s) 42, 86, 129, 134
CviKI_1 RGCY 4 cut(s) 42, 86, 129, 134
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 183, 381
DpnI GATC 2 cut(s) 71, 107
DpnII GATC 2 cut(s) 69, 105
Eco130I CCWWGG 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 108, 124
EcoT14I CCWWGG 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 2 cut(s) 143, 180
FaiI YATR 4 cut(s) 90, 141, 178, 441
FaqI GGGAC 1 cut(s) 210
FatI CATG 2 cut(s) 139, 176
FauI CCCGC 1 cut(s) 187
FokI GGATG 2 cut(s) 122, 440
FspBI CTAG 1 cut(s) 155
GlaI GCGC 1 cut(s) 65
HaeIII GGCC 1 cut(s) 129
HhaI GCGC 1 cut(s) 66
Hin1II CATG 2 cut(s) 143, 180
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HinfI GANTC 2 cut(s) 114, 329
HphI GGTGA 1 cut(s) 151
Hpy188I TCNGA 4 cut(s) 119, 184, 249, 382
Hpy188III TCNNGA 2 cut(s) 326, 359
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 2 cut(s) 23, 364
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 183, 381
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 2 cut(s) 143, 180
HspAI GCGC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 69, 105
LpnPI CCDG 7 cut(s) 95, 111, 122, 138, 249, 258, 372
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 155
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 4 cut(s) 58, 157, 259, 442
MalI GATC 2 cut(s) 71, 107
MboI GATC 2 cut(s) 69, 105
MboII GAAGA 3 cut(s) 19, 22, 64
MnlI CCTC 2 cut(s) 157, 355
MseI TTAA 1 cut(s) 423
MspR9I CCNGG 2 cut(s) 110, 126
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 2 cut(s) 110, 126
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 218
NcoI CCATGG 1 cut(s) 139
NdeII GATC 2 cut(s) 69, 105
NlaIII CATG 2 cut(s) 143, 180
NmuCI GTSAC 2 cut(s) 157, 259
NspV TTCGAA 1 cut(s) 368
PctI GAATGC 1 cut(s) 457
PfeI GAWTC 2 cut(s) 114, 329
Ppu21I YACGTR 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 108, 124
PspGI CCWGG 2 cut(s) 108, 124
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SaqAI TTAA 1 cut(s) 423
Sau3AI GATC 2 cut(s) 69, 105
ScaI AGTACT 1 cut(s) 188
ScrFI CCNGG 2 cut(s) 110, 126
SetI ASST 5 cut(s) 44, 168, 301, 366, 413
SfaNI GCATC 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 368
SpeI ACTAGT 1 cut(s) 154
SsiI CCGC 1 cut(s) 194
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 2 cut(s) 108, 124
StyI CCWWGG 1 cut(s) 139
TaiI ACGT 1 cut(s) 413
TaqI TCGA 3 cut(s) 50, 336, 368
TaqII GACCGA 1 cut(s) 322
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 2 cut(s) 114, 329
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 2 cut(s) 157, 259
Tsp45I GTSAC 2 cut(s) 157, 259
TspRI CASTG 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XspI CTAG 1 cut(s) 155
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.