MD05G1170600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015343
Physical Location & Seq
Reverse (-)
30002219 .. 30003481
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1170600.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGTCAGAATTTTCTTTCCAACCAAAGACGTCCAAGAAAATCAAAATCTCTAGTCTTCTTCTCGCTGACTTGTTCAATCTTTGTTTCTGCATTTTGTCGCACCCTCTTTACTTCTCCTACTTCATATTTTTCTCACCCTACCTCCTCAAGCTCCTTTCTTTTCTGTCTCCTCTTTTCATCACCACAGCTCTTCTTGTGGTTGCTTATCTAACGGTCCAACACGACGTTGCTGATTCCAAATCGTCGAAAGTCAAATTGGGTTGTCTTGTCACTGCGTACCAAACTGTTTTGGAAGGTTTGCACAAGTTTAGAGTTGATGATAGTGATGGCGGTGATCGTGATCATGATCATGAAGAATTCCGGTCTTCCGTGGAGCTCGAAGCGTATAGAATTGTGTTTGACACGTCCACGTTCGAAATCAACGAGACCCCAGCTGAGAAAAATTGCTCACAACTCTTTGAAGCACAAGTTGAAGATGTGTCTAGTCATGAAGCTAATTGTGCTTCTGATGTTTCTGATGCTGCAAGTGATGTTTTCGACGACAAGTTCGAAATCCCAGCTGAGGCATTCGTAGAAGAAGAAGAAGAGAATTGGCCAGAAAACTTGTGCAAAAAGGAAGAGGAAGAAGTGATCAAGCCACCACCAAGCACAGAGTCCAAGAAAGTTGAAGAACAAGAAGGAAAAGAAAAAAGGTTTATGAGAAGAGGAGAATCCAAGGATTATTTGGGTGATGGAGGTGAGTTAAATTCCAAATTATCCAGGGTAAATTCCCAAACCCTTGGAGCAAATCTTTGGGAATCCAGGTTAAATCCCCAAACCCTTGGAGCAAATGTTGGAGAATGTTACTACAACATGAGTCCAAATCTTGGAAGTTTTGGATCAATGAGGAAAGAGAAAGAGTGGAGAAGGACACTGGCATGCAAGCTTTTTGAGGAGAGGCATCAAAATGTGGATGGAGGAGGAGGAGGAGGAGGAGAAGGGATGGACATGCTTTGGGAGACATATGATGAGACAGAATCATTAAAAGTGGTGAAAGGTAAAAAAAGCAAGTCAAAGAAGGGGAAAGATGGGAAAGCTGAAAGCAACGAGGGTGAAGAGGAAGAGTTTGATGGGCAATTGTGTTGCTTGCAGGCATTGAAATTCTCAGCTGGGAAGATGAATTTGGGGATGGGAAGGCCTAATCTTGTCAAGTTTTCCAAGGCACTAAAAGGGTTTGGATGGTTACACCATGTCACCAAGCATGGCAAGAAGGGGCACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

47.22

Weight (kDa)

5.35

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25130
fragaria_vesca FvH4_2g18490
malus_domestica MD05G1170600.v1.1 MD10G1158800.v1.1
prunus_persica Prupe.8G197900_v2.0.a1
pyrus_communis pycom05g15780
rosa_chinensis RchiOBHm_Chr6g0284001
rosa_laevigata RLG00000012779
rosa_multiflora Rmu_sc0003102.1_g000005 Rmu_ssc0000442.1_g000040
rosa_rugosa Rorug06G0159200
rosa_samantha Rh6AG272600 Rh6BG273200 Rh6CG274400 Rh6DG267600
rosa_wichuraiana Rw6G023470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 1254
AccB7I CCANNNNNTGG 1 cut(s) 866
AciI CCGC 1 cut(s) 330
AclWI GGATC 1 cut(s) 886
AcoI YGGCCR 1 cut(s) 593
AcsI RAATTY 6 cut(s) 8, 356, 745, 766, 1139, 1159
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 2 cut(s) 562, 866
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 5 cut(s) 76, 461, 473, 668, 1138
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 2 cut(s) 758, 800
AjuI GAANNNNNNNTTGG 4 cut(s) 239, 271, 1145, 1177
AluBI AGCT 9 cut(s) 151, 188, 376, 434, 494, 560, 925, 1076, 1148
AluI AGCT 9 cut(s) 151, 188, 376, 434, 494, 560, 925, 1076, 1148
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 4 cut(s) 171, 419, 992, 1004
AlwI GGATC 1 cut(s) 886
AoxI GGCC 2 cut(s) 593, 1175
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 6 cut(s) 8, 356, 745, 766, 1139, 1159
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 8 cut(s) 126, 172, 344, 740, 749, 1042, 1103, 1225
AsuII TTCGAA 2 cut(s) 414, 549
AvaII GGWCC 1 cut(s) 214
BaeGI GKGCMC 1 cut(s) 1257
BalI TGGCCA 1 cut(s) 595
BanI GGYRCC 1 cut(s) 1254
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 47, 357
Bbv12I GWGCWC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 508
BccI CCATC 8 cut(s) 320, 725, 947, 976, 1061, 1103, 1162, 1212
BciT130I CCWGG 2 cut(s) 760, 802
BclI TGATCA 3 cut(s) 340, 346, 630
BcoDI GTCTC 4 cut(s) 171, 419, 992, 1004
BfaI CTAG 2 cut(s) 51, 483
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 760, 802
Bme18I GGWCC 1 cut(s) 214
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 1256
BmrFI CCNGG 2 cut(s) 760, 802
BmsI GCATC 2 cut(s) 508, 949
BpiI GAAGAC 2 cut(s) 47, 357
Bpu10I CCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 2 cut(s) 414, 549
BpuEI CTTGAG 1 cut(s) 131
BsaBI GATNNNNATC 2 cut(s) 339, 345
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 6 cut(s) 369, 714, 759, 778, 820, 1197
BsaWI WCCGGW 1 cut(s) 360
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 816, 846
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 866
Bse1I ACTGG 1 cut(s) 918
Bse8I GATNNNNATC 2 cut(s) 339, 345
BseBI CCWGG 2 cut(s) 760, 802
BseDI CCNNGG 6 cut(s) 369, 714, 759, 778, 820, 1197
BseGI GGATG 4 cut(s) 958, 987, 1173, 1223
BseJI GATNNNNATC 2 cut(s) 339, 345
BseLI CCNNNNNNNGG 2 cut(s) 562, 866
BseMII CTCAG 3 cut(s) 426, 552, 1158
BseNI ACTGG 1 cut(s) 918
BseSI GKGCMC 1 cut(s) 1257
BseXI GCAGC 1 cut(s) 508
BseYI CCCAGC 3 cut(s) 430, 556, 1148
BshFI GGCC 2 cut(s) 595, 1177
BshNI GGYRCC 1 cut(s) 1254
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 1 cut(s) 361
BslI CCNNNNNNNGG 2 cut(s) 562, 866
BsmAI GTCTC 4 cut(s) 171, 419, 992, 1004
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 2 cut(s) 595, 1177
Bso31I GGTCTC 1 cut(s) 419
Bsp119I TTCGAA 2 cut(s) 414, 549
Bsp1286I GDGCHC 2 cut(s) 378, 1257
Bsp143I GATC 5 cut(s) 334, 340, 346, 630, 878
BspACI CCGC 1 cut(s) 330
BspANI GGCC 2 cut(s) 595, 1177
BspCNI CTCAG 3 cut(s) 427, 553, 1157
BspHI TCATGA 3 cut(s) 343, 349, 487
BspLI GGNNCC 1 cut(s) 1256
BspPI GGATC 1 cut(s) 886
BspQI GCTCTTC 1 cut(s) 195
BspT104I TTCGAA 2 cut(s) 414, 549
BspT107I GGYRCC 1 cut(s) 1254
BspTNI GGTCTC 1 cut(s) 419
BsrI ACTGG 1 cut(s) 918
BssECI CCNNGG 6 cut(s) 369, 714, 759, 778, 820, 1197
BssMI GATC 5 cut(s) 334, 340, 346, 630, 878
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 4 cut(s) 714, 778, 820, 1197
Bst2UI CCWGG 2 cut(s) 760, 802
Bst4CI ACNGT 2 cut(s) 214, 286
Bst6I CTCTTC 6 cut(s) 195, 579, 612, 697, 1089, 1095
BstACI GRCGYC 1 cut(s) 29
BstBI TTCGAA 2 cut(s) 414, 549
BstC8I GCNNGC 4 cut(s) 919, 923, 1127, 1131
BstDEI CTNAG 3 cut(s) 435, 561, 1144
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 4 cut(s) 958, 987, 1173, 1223
BstKTI GATC 5 cut(s) 337, 343, 349, 633, 881
BstMAI GTCTC 4 cut(s) 171, 419, 992, 1004
BstMBI GATC 5 cut(s) 334, 340, 346, 630, 878
BstMWI GCNNNNNNNGC 1 cut(s) 500
BstNI CCWGG 2 cut(s) 760, 802
BstNSI RCATGY 2 cut(s) 921, 991
BstSCI CCNGG 2 cut(s) 758, 800
BstSLI GKGCMC 1 cut(s) 1257
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 2 cut(s) 47, 357
BstXI CCANNNNNNTGG 2 cut(s) 779, 821
BsuRI GGCC 2 cut(s) 595, 1177
BtgI CCRYGG 1 cut(s) 369
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 4 cut(s) 958, 987, 1173, 1223
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 270, 911
Cac8I GCNNGC 4 cut(s) 919, 923, 1127, 1131
CciI TCATGA 3 cut(s) 343, 349, 487
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 277
CviAII CATG 8 cut(s) 344, 350, 488, 853, 918, 988, 1229, 1241
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 435, 561, 1144
DpnI GATC 5 cut(s) 336, 342, 348, 632, 880
DpnII GATC 5 cut(s) 334, 340, 346, 630, 878
EaeI YGGCCR 1 cut(s) 593
Eam1104I CTCTTC 6 cut(s) 195, 579, 612, 697, 1089, 1095
EarI CTCTTC 6 cut(s) 195, 579, 612, 697, 1089, 1095
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 4 cut(s) 714, 778, 820, 1197
Eco147I AGGCCT 1 cut(s) 1177
Eco24I GRGCYC 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 758, 800
EcoT14I CCWWGG 4 cut(s) 714, 778, 820, 1197
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 4 cut(s) 714, 778, 820, 1197
FaeI CATG 8 cut(s) 347, 353, 491, 856, 921, 991, 1232, 1244
FatI CATG 8 cut(s) 343, 349, 487, 852, 917, 987, 1228, 1240
FauNDI CATATG 1 cut(s) 1003
FbaI TGATCA 3 cut(s) 340, 346, 630
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 4 cut(s) 965, 994, 1180, 1230
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 2 cut(s) 51, 483
GluI GCNGC 1 cut(s) 522
GsaI CCCAGC 3 cut(s) 434, 560, 1152
HaeIII GGCC 2 cut(s) 595, 1177
HapII CCGG 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 8 cut(s) 347, 353, 491, 856, 921, 991, 1232, 1244
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 6 cut(s) 233, 653, 710, 797, 856, 1016
HpaII CCGG 1 cut(s) 361
HphI GGTGA 8 cut(s) 126, 172, 344, 740, 749, 1042, 1103, 1225
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 3 cut(s) 7, 508, 517
Hpy188III TCNNGA 4 cut(s) 338, 344, 350, 488
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 3 cut(s) 227, 247, 542
HpyAV CCTTC 7 cut(s) 287, 671, 900, 971, 1051, 1167, 1243
HpyCH4III ACNGT 2 cut(s) 214, 286
HpyCH4IV ACGT 4 cut(s) 29, 225, 404, 410
HpyCH4V TGCA 6 cut(s) 90, 301, 524, 609, 921, 1129
HpyF10VI GCNNNNNNNGC 1 cut(s) 500
HpyF3I CTNAG 3 cut(s) 435, 561, 1144
HpySE526I ACGT 4 cut(s) 29, 225, 404, 410
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 8 cut(s) 347, 353, 491, 856, 921, 991, 1232, 1244
Ksp22I TGATCA 3 cut(s) 340, 346, 630
Kzo9I GATC 5 cut(s) 334, 340, 346, 630, 878
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 4 cut(s) 156, 373, 782, 824
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 2 cut(s) 508, 949
MaeI CTAG 2 cut(s) 51, 483
MaeII ACGT 4 cut(s) 29, 225, 404, 410
MaeIII GTNAC 4 cut(s) 268, 842, 1221, 1231
MalI GATC 5 cut(s) 336, 342, 348, 632, 880
MboI GATC 5 cut(s) 334, 340, 346, 630, 878
MfeI CAATTG 1 cut(s) 1115
MhlI GDGCHC 2 cut(s) 378, 1257
MlsI TGGCCA 1 cut(s) 595
MluNI TGGCCA 1 cut(s) 595
MlyI GAGTC 2 cut(s) 662, 865
MmeI TCCRAC 3 cut(s) 43, 241, 814
Mox20I TGGCCA 1 cut(s) 595
MscI TGGCCA 1 cut(s) 595
MseI TTAA 3 cut(s) 743, 806, 1022
MslI CAYNNNNRTG 3 cut(s) 348, 916, 945
Msp20I TGGCCA 1 cut(s) 595
MspA1I CMGCKG 3 cut(s) 434, 560, 1148
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 2 cut(s) 760, 802
MunI CAATTG 1 cut(s) 1115
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 2 cut(s) 760, 802
MwoI GCNNNNNNNGC 1 cut(s) 500
NdeI CATATG 1 cut(s) 1003
NdeII GATC 5 cut(s) 334, 340, 346, 630, 878
NlaIII CATG 8 cut(s) 347, 353, 491, 856, 921, 991, 1232, 1244
NlaIV GGNNCC 1 cut(s) 1256
NmuCI GTSAC 2 cut(s) 268, 1231
NspI RCATGY 2 cut(s) 921, 991
NspV TTCGAA 2 cut(s) 414, 549
PaeI GCATGC 1 cut(s) 921
PagI TCATGA 3 cut(s) 343, 349, 487
PceI AGGCCT 1 cut(s) 1177
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 546
PctI GAATGC 1 cut(s) 566
PfeI GAWTC 4 cut(s) 233, 710, 797, 1016
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 1 cut(s) 523
PleI GAGTC 2 cut(s) 661, 864
PpsI GAGTC 2 cut(s) 661, 864
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 758, 800
PspFI CCCAGC 3 cut(s) 430, 556, 1148
PspGI CCWGG 2 cut(s) 758, 800
PspN4I GGNNCC 1 cut(s) 1256
PspPI GGNCC 1 cut(s) 214
PvuII CAGCTG 3 cut(s) 434, 560, 1148
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 3 cut(s) 348, 916, 945
SacI GAGCTC 1 cut(s) 378
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 743, 806, 1022
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 5 cut(s) 334, 340, 346, 630, 878
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 2 cut(s) 662, 865
ScrFI CCNGG 2 cut(s) 760, 802
SduI GDGCHC 2 cut(s) 378, 1257
SfaNI GCATC 2 cut(s) 508, 949
SfuI TTCGAA 2 cut(s) 414, 549
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 3 cut(s) 348, 916, 945
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SphI GCATGC 1 cut(s) 921
SseBI AGGCCT 1 cut(s) 1177
SsiI CCGC 1 cut(s) 330
SspMI CTAG 2 cut(s) 51, 483
SstI GAGCTC 1 cut(s) 378
StuI AGGCCT 1 cut(s) 1177
StyD4I CCNGG 2 cut(s) 758, 800
StyI CCWWGG 4 cut(s) 714, 778, 820, 1197
TaaI ACNGT 2 cut(s) 214, 286
TaiI ACGT 4 cut(s) 32, 228, 407, 413
TaqI TCGA 5 cut(s) 245, 378, 414, 537, 549
TfiI GAWTC 4 cut(s) 233, 710, 797, 1016
Tru1I TTAA 3 cut(s) 743, 806, 1022
Tru9I TTAA 3 cut(s) 743, 806, 1022
TscAI CASTG 2 cut(s) 277, 918
TseFI GTSAC 2 cut(s) 268, 1231
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 268, 1231
TspDTI ATGAA 5 cut(s) 112, 166, 366, 504, 1172
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 2 cut(s) 277, 918
Van91I CCANNNNNTGG 1 cut(s) 866
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 6 cut(s) 8, 356, 745, 766, 1139, 1159
XceI RCATGY 2 cut(s) 921, 991
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XspI CTAG 2 cut(s) 51, 483
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.