MD10G1158800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015343
Physical Location & Seq
Reverse (-)
24844158 .. 24845414
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1158800.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGTCAGATTTTTCTTTCCAACCAAACACGTCCAAGAAAACCAAAATCTCTAGTCTTCTCGCTGACTTGTTCAGTCTTTGTTTCTGCATTTTGTCACACCCTCTTTACGTCTCCTACTTCATTTTTTTCTCACCGTACCTCCTCAAGCTCCTTTCTTTTCTATCTCCTCTCTTTGTCACCACAGCTCTTCTTGTGTTGGCTTATCTCACTGTCCAAAACGATGTTGCTAATTCCGATTCGTCGAAATTTAAACTGGGTTGTCTTGTCACCGCCTACCAAACTGTTTTGGAAGGGTTGAAGAAGTCTAAAGTTGATGATAGTGATCGTGGTGATCGTGATCATGACCGCGAAGAATTTCGGTCTGCTGTGGAGCTCGAAGCGTATAAAATCGTGTTTGACACGTCGACGTTCGAAATCAACGAGACCCCAGTTGAAGAAAATTGCTCACAAGTCTTTGAATCACAAGTTGAAGATGTGTCAAGTCATGAAGCTAATGGTGCTTCTGATGCTTTTGATGCTGCAAGTGATGTTTTCGAAGACAAGTTCGAAATCCCAGCTGAGGCATTCGTACAAGAAGAAGAGAATTGGTCAGAAAACTTGTGCACAAAGGAAGAGGAAGAAGAAGTGATCAGGCCACCACCAAGCACAGAATCCAAGAAAGTTGAAGAACAAGAAGGAAAAGGAAAGAGGTCTGTGAGAAGAAGAGAATCCAAGGATTATTTGGGTGATGGAAGTGAGTTAAATTCCAAACTATCCATGGTAAATTCTCAAACCCTAGGAGCAAATCTTGGGGAATCCAGGTTAAATCCCCAAACCCTAGGATCAAATGTTGGAGAATGTTACTTCAACACGAGTCCAAATCTTGGAAGTTTTGGATCAATGAGGAAAGAGAAGGAGTGGAGGAGGACACTGGCATGCAAGCTTTTTGAGGAGAGGCATCAAAATGTGGATGGAGGAGAAGGAGGAGAGGGGATGGACATGCTTTGGGAGACATATGAAGAGACAGAATCATTAAAAGTCGTGAAAGGTAACAAAAGTAAGTCAAAGAAGGGGAAAAATGGGAAAGTTGAGAGCAATGATGAAGTGGAAGAGGGAGAGTTTGATGGGCAGTTGTGCTGCTTGCAAGCATTGAAACTCTCAGCTGGGAAGATGAATTTGGGAATGGGGAGGCCTAATCTTGTCAAGTTTTCCAAGGCCCTAAAAGGGTTTGGATGGTTGCACCATGTCACCAAGCATGGCAAGAAGGGGCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

46.82

Weight (kDa)

5.16

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25130
fragaria_vesca FvH4_2g18490
malus_domestica MD05G1170600.v1.1 MD10G1158800.v1.1
prunus_persica Prupe.8G197900_v2.0.a1
pyrus_communis pycom05g15780
rosa_chinensis RchiOBHm_Chr6g0284001
rosa_laevigata RLG00000012779
rosa_multiflora Rmu_sc0003102.1_g000005 Rmu_ssc0000442.1_g000040
rosa_rugosa Rorug06G0159200
rosa_samantha Rh6AG272600 Rh6BG273200 Rh6CG274400 Rh6DG267600
rosa_wichuraiana Rw6G023470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 863
AccI GTMKAC 1 cut(s) 404
AccII CGCG 1 cut(s) 348
AciI CCGC 2 cut(s) 270, 346
AclWI GGATC 2 cut(s) 829, 883
AcsI RAATTY 5 cut(s) 245, 353, 742, 763, 1153
AfaI GTAC 2 cut(s) 137, 570
AfiI CCNNNNNNNGG 3 cut(s) 559, 863, 1203
AflIII ACRYGT 2 cut(s) 27, 399
AgsI TTSAA 7 cut(s) 298, 434, 458, 470, 665, 847, 1132
AjiI CACGTC 2 cut(s) 30, 402
AjnI CCWGG 1 cut(s) 797
AjuI GAANNNNNNNTTGG 2 cut(s) 1139, 1171
AluBI AGCT 7 cut(s) 148, 185, 373, 491, 557, 922, 1142
AluI AGCT 7 cut(s) 148, 185, 373, 491, 557, 922, 1142
Alw21I GWGCWC 2 cut(s) 375, 605
Alw26I GTCTC 4 cut(s) 115, 416, 983, 995
Alw44I GTGCAC 1 cut(s) 601
AlwI GGATC 2 cut(s) 829, 883
AoxI GGCC 3 cut(s) 632, 1169, 1194
ApaLI GTGCAC 1 cut(s) 601
ApeKI GCWGC 2 cut(s) 518, 1116
ApoI RAATTY 5 cut(s) 245, 353, 742, 763, 1153
Asp700I GAANNNNTTC 1 cut(s) 354
AspA2I CCTAGG 2 cut(s) 775, 817
AspS9I GGNCC 1 cut(s) 1195
AsuHPI GGTGA 6 cut(s) 123, 169, 259, 341, 737, 1219
AsuII TTCGAA 3 cut(s) 411, 534, 546
AvrII CCTAGG 2 cut(s) 775, 817
BaeGI GKGCMC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 375
BauI CACGAG 1 cut(s) 850
BbsI GAAGAC 2 cut(s) 47, 543
Bbv12I GWGCWC 2 cut(s) 375, 605
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 505, 1103
BccI CCATC 5 cut(s) 722, 944, 967, 1097, 1206
BciT130I CCWGG 1 cut(s) 799
BclI TGATCA 2 cut(s) 337, 627
BcoDI GTCTC 4 cut(s) 115, 416, 983, 995
BfaI CTAG 3 cut(s) 51, 776, 818
BisI GCNGC 2 cut(s) 519, 1117
BlnI CCTAGG 2 cut(s) 775, 817
BlsI GCNGC 2 cut(s) 520, 1118
Bme1390I CCNGG 1 cut(s) 799
BmgBI CACGTC 2 cut(s) 30, 402
BmgT120I GGNCC 1 cut(s) 1195
BmrFI CCNGG 1 cut(s) 799
BmrI ACTGGG 2 cut(s) 263, 422
BmsI GCATC 3 cut(s) 496, 505, 946
BmuI ACTGGG 2 cut(s) 263, 422
BpiI GAAGAC 2 cut(s) 47, 543
Bpu10I CCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 3 cut(s) 411, 534, 546
BpuEI CTTGAG 1 cut(s) 128
BsaBI GATNNNNATC 2 cut(s) 321, 336
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 5 cut(s) 711, 756, 775, 817, 1191
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 3 cut(s) 559, 863, 1203
Bse1I ACTGG 3 cut(s) 258, 428, 915
Bse3DI GCAATG 1 cut(s) 1081
Bse8I GATNNNNATC 2 cut(s) 321, 336
BseBI CCWGG 1 cut(s) 799
BseDI CCNNGG 5 cut(s) 711, 756, 775, 817, 1191
BseGI GGATG 3 cut(s) 955, 978, 1217
BseJI GATNNNNATC 2 cut(s) 321, 336
BseLI CCNNNNNNNGG 3 cut(s) 559, 863, 1203
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 2 cut(s) 549, 1152
BseNI ACTGG 3 cut(s) 258, 428, 915
BseRI GAGGAG 6 cut(s) 131, 156, 916, 944, 969, 978
BseSI GKGCMC 1 cut(s) 605
BseXI GCAGC 2 cut(s) 505, 1103
BseYI CCCAGC 2 cut(s) 553, 1142
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 3 cut(s) 634, 1171, 1196
BsiHKAI GWGCWC 2 cut(s) 375, 605
BslI CCNNNNNNNGG 3 cut(s) 559, 863, 1203
BsmAI GTCTC 4 cut(s) 115, 416, 983, 995
BsmBI CGTCTC 1 cut(s) 115
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 3 cut(s) 634, 1171, 1196
Bso31I GGTCTC 1 cut(s) 416
Bsp119I TTCGAA 3 cut(s) 411, 534, 546
Bsp1286I GDGCHC 2 cut(s) 375, 605
Bsp143I GATC 6 cut(s) 322, 331, 337, 627, 821, 875
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 2 cut(s) 270, 346
BspANI GGCC 3 cut(s) 634, 1171, 1196
BspCNI CTCAG 2 cut(s) 550, 1151
BspFNI CGCG 1 cut(s) 348
BspHI TCATGA 2 cut(s) 340, 484
BspPI GGATC 2 cut(s) 829, 883
BspQI GCTCTTC 1 cut(s) 192
BspT104I TTCGAA 3 cut(s) 411, 534, 546
BspTNI GGTCTC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 1081
BsrI ACTGG 3 cut(s) 258, 428, 915
BssECI CCNNGG 5 cut(s) 711, 756, 775, 817, 1191
BssMI GATC 6 cut(s) 322, 331, 337, 627, 821, 875
BssSI CACGAG 1 cut(s) 850
BssT1I CCWWGG 5 cut(s) 711, 756, 775, 817, 1191
Bst2BI CACGAG 1 cut(s) 850
Bst2UI CCWGG 1 cut(s) 799
Bst4CI ACNGT 3 cut(s) 135, 211, 283
Bst6I CTCTTC 6 cut(s) 192, 573, 606, 697, 993, 1083
BstBI TTCGAA 3 cut(s) 411, 534, 546
BstC8I GCNNGC 4 cut(s) 916, 920, 1121, 1125
BstDEI CTNAG 2 cut(s) 558, 1138
BstDSI CCRYGG 1 cut(s) 756
BstF5I GGATG 3 cut(s) 955, 978, 1217
BstFNI CGCG 1 cut(s) 348
BstKTI GATC 6 cut(s) 325, 334, 340, 630, 824, 878
BstMAI GTCTC 4 cut(s) 115, 416, 983, 995
BstMBI GATC 6 cut(s) 322, 331, 337, 627, 821, 875
BstMWI GCNNNNNNNGC 3 cut(s) 497, 506, 515
BstNI CCWGG 1 cut(s) 799
BstNSI RCATGY 2 cut(s) 918, 982
BstSCI CCNGG 1 cut(s) 797
BstSLI GKGCMC 1 cut(s) 605
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 505, 1103
BstV2I GAAGAC 2 cut(s) 47, 543
BsuRI GGCC 3 cut(s) 634, 1171, 1196
BtgI CCRYGG 1 cut(s) 756
BtrI CACGTC 2 cut(s) 30, 402
BtsCI GGATG 3 cut(s) 955, 978, 1217
BtsIMutI CAGTG 3 cut(s) 207, 908, 1252
Cac8I GCNNGC 4 cut(s) 916, 920, 1121, 1125
CciI TCATGA 2 cut(s) 340, 484
Cfr13I GGNCC 1 cut(s) 1195
Csp6I GTAC 2 cut(s) 136, 569
CviAII CATG 7 cut(s) 341, 485, 757, 915, 979, 1223, 1235
CviQI GTAC 2 cut(s) 136, 569
DdeI CTNAG 2 cut(s) 558, 1138
DpnI GATC 6 cut(s) 324, 333, 339, 629, 823, 877
DpnII GATC 6 cut(s) 322, 331, 337, 627, 821, 875
DraI TTTAAA 1 cut(s) 250
Eam1104I CTCTTC 6 cut(s) 192, 573, 606, 697, 993, 1083
EarI CTCTTC 6 cut(s) 192, 573, 606, 697, 993, 1083
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 5 cut(s) 711, 756, 775, 817, 1191
Eco147I AGGCCT 1 cut(s) 1171
Eco24I GRGCYC 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 416
Eco53kI GAGCTC 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 1195
EcoRII CCWGG 1 cut(s) 797
EcoT14I CCWWGG 5 cut(s) 711, 756, 775, 817, 1191
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 5 cut(s) 711, 756, 775, 817, 1191
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 7 cut(s) 344, 488, 760, 918, 982, 1226, 1238
FatI CATG 7 cut(s) 340, 484, 756, 914, 978, 1222, 1234
FauNDI CATATG 1 cut(s) 994
FbaI TGATCA 2 cut(s) 337, 627
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 2 cut(s) 519, 1117
FokI GGATG 3 cut(s) 962, 985, 1224
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 2 cut(s) 519, 1117
FspBI CTAG 3 cut(s) 51, 776, 818
GluI GCNGC 2 cut(s) 519, 1117
GsaI CCCAGC 2 cut(s) 557, 1146
HaeIII GGCC 3 cut(s) 634, 1171, 1196
Hin1II CATG 7 cut(s) 344, 488, 760, 918, 982, 1226, 1238
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 7 cut(s) 236, 458, 650, 707, 794, 853, 1007
HphI GGTGA 6 cut(s) 123, 169, 259, 341, 737, 1219
Hpy166II GTNNAC 2 cut(s) 405, 603
Hpy188I TCNGA 4 cut(s) 7, 235, 505, 592
Hpy188III TCNNGA 4 cut(s) 335, 341, 485, 1021
Hpy8I GTNNAC 2 cut(s) 405, 603
Hpy99I CGWCG 3 cut(s) 244, 406, 409
HpyAV CCTTC 6 cut(s) 284, 668, 886, 953, 1042, 1237
HpyCH4III ACNGT 3 cut(s) 135, 211, 283
HpyCH4IV ACGT 4 cut(s) 29, 108, 401, 407
HpyCH4V TGCA 6 cut(s) 87, 521, 603, 918, 1123, 1219
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 506, 515
HpyF3I CTNAG 2 cut(s) 558, 1138
HpySE526I ACGT 4 cut(s) 29, 108, 401, 407
Hsp92II CATG 7 cut(s) 344, 488, 760, 918, 982, 1226, 1238
Ksp22I TGATCA 2 cut(s) 337, 627
Kzo9I GATC 6 cut(s) 322, 331, 337, 627, 821, 875
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 3 cut(s) 153, 370, 779
LpnPI CCDG 8 cut(s) 239, 441, 567, 616, 784, 811, 896, 1128
Lsp1109I GCAGC 2 cut(s) 505, 1103
LweI GCATC 3 cut(s) 496, 505, 946
MaeI CTAG 3 cut(s) 51, 776, 818
MaeII ACGT 4 cut(s) 29, 108, 401, 407
MaeIII GTNAC 6 cut(s) 93, 175, 265, 839, 1028, 1225
MalI GATC 6 cut(s) 324, 333, 339, 629, 823, 877
MboI GATC 6 cut(s) 322, 331, 337, 627, 821, 875
MhlI GDGCHC 2 cut(s) 375, 605
MluCI AATT 8 cut(s) 229, 245, 353, 439, 583, 742, 763, 1153
MlyI GAGTC 1 cut(s) 862
MmeI TCCRAC 2 cut(s) 43, 811
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 4 cut(s) 249, 740, 803, 1013
MslI CAYNNNNRTG 2 cut(s) 913, 942
MspA1I CMGCKG 2 cut(s) 557, 1142
MspR9I CCNGG 1 cut(s) 799
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 1 cut(s) 799
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 497, 506, 515
NcoI CCATGG 1 cut(s) 756
NdeI CATATG 1 cut(s) 994
NdeII GATC 6 cut(s) 322, 331, 337, 627, 821, 875
NlaIII CATG 7 cut(s) 344, 488, 760, 918, 982, 1226, 1238
NmuCI GTSAC 4 cut(s) 93, 175, 265, 1225
NspI RCATGY 2 cut(s) 918, 982
NspV TTCGAA 3 cut(s) 411, 534, 546
PaeI GCATGC 1 cut(s) 918
PagI TCATGA 2 cut(s) 340, 484
PceI AGGCCT 1 cut(s) 1171
PciSI GCTCTTC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 2 cut(s) 331, 417
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 6 cut(s) 236, 458, 650, 707, 794, 1007
PflMI CCANNNNNTGG 1 cut(s) 863
PkrI GCNGC 2 cut(s) 520, 1118
PleI GAGTC 1 cut(s) 861
PpsI GAGTC 1 cut(s) 861
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 797
PspFI CCCAGC 2 cut(s) 553, 1142
PspGI CCWGG 1 cut(s) 797
PspPI GGNCC 1 cut(s) 1195
PvuII CAGCTG 2 cut(s) 557, 1142
RsaI GTAC 2 cut(s) 137, 570
RsaNI GTAC 2 cut(s) 136, 569
RseI CAYNNNNRTG 2 cut(s) 913, 942
SacI GAGCTC 1 cut(s) 375
SalI GTCGAC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 4 cut(s) 249, 740, 803, 1013
SatI GCNGC 2 cut(s) 519, 1117
Sau3AI GATC 6 cut(s) 322, 331, 337, 627, 821, 875
Sau96I GGNCC 1 cut(s) 1195
SchI GAGTC 1 cut(s) 862
ScrFI CCNGG 1 cut(s) 799
SduI GDGCHC 2 cut(s) 375, 605
SfaNI GCATC 3 cut(s) 496, 505, 946
SfuI TTCGAA 3 cut(s) 411, 534, 546
SgrDI CGTCGACG 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 913, 942
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SphI GCATGC 1 cut(s) 918
Sse9I AATT 8 cut(s) 229, 245, 353, 439, 583, 742, 763, 1153
SseBI AGGCCT 1 cut(s) 1171
SsiI CCGC 2 cut(s) 270, 346
SspMI CTAG 3 cut(s) 51, 776, 818
SstI GAGCTC 1 cut(s) 375
StuI AGGCCT 1 cut(s) 1171
StyD4I CCNGG 1 cut(s) 797
StyI CCWWGG 5 cut(s) 711, 756, 775, 817, 1191
TaaI ACNGT 3 cut(s) 135, 211, 283
TaiI ACGT 4 cut(s) 32, 111, 404, 410
TaqI TCGA 6 cut(s) 242, 375, 404, 411, 534, 546
TaqII GACCGA 1 cut(s) 348
TasI AATT 8 cut(s) 229, 245, 353, 439, 583, 742, 763, 1153
TfiI GAWTC 6 cut(s) 236, 458, 650, 707, 794, 1007
Tru1I TTAA 4 cut(s) 249, 740, 803, 1013
Tru9I TTAA 4 cut(s) 249, 740, 803, 1013
TscAI CASTG 2 cut(s) 214, 915
TseFI GTSAC 4 cut(s) 93, 175, 265, 1225
TseI GCWGC 2 cut(s) 518, 1116
Tsp45I GTSAC 4 cut(s) 93, 175, 265, 1225
TspDTI ATGAA 5 cut(s) 109, 501, 1011, 1095, 1166
TspRI CASTG 2 cut(s) 214, 915
Van91I CCANNNNNTGG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 601
XapI RAATTY 5 cut(s) 245, 353, 742, 763, 1153
XceI RCATGY 2 cut(s) 918, 982
XcmI CCANNNNNNNNNTGG 2 cut(s) 718, 754
XmaJI CCTAGG 2 cut(s) 775, 817
XmiI GTMKAC 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 3 cut(s) 51, 776, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.