Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016519

Physical Location & Seq

Genomic Coordinates Reverse (-)

31464840 .. 31466333

Gene Structure 1494 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1186200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGGCGGCTGCTATTTCTTGCTCAGCCACCGACCTCCGACCCCTTTTGGGTGCAGCTGCCAACGCCACTGCGGCTGCAGAATACATCTGCGGCCGCTTTGATGCTGTGTCAAACAAGTTTGTCGACACAGCCTATGCTGTGGACAACACCTATCTTCTGTTCTCTGCCTACCTTGTTTTTGCAATGCAGCTAGGGTTTGCCATGCTCTGCGCAGGATCAGTTCGCGCGAAAAATACCATGAACATTATGCTCACCAACGTCCTTGACGCTGCCACGGGCGGCATCTTCTACTACCTCTTTGGATTTGCATTGGCCTTTGGCACACCGTCAAACGGTTTCATAGGGCAGCACTTCTTCGGCCTCAGCAAGTTTCCCTCTCAATCTTTTGATTACGGTTACTTCCTTTATCAATGGGCTTTTGCCATTGCAGCTGCTGGGATCACAAGTGGTTCCATTGCTGAACGAACCCAATTCGTCGCGTACTTGATTTATTCGTCCTTCCTGACTGGATTTGTCTACCCAATTGTGTCCCATTGGTTCTGGTCTGCTGAAGGATGGGCTAGCCCGGCACGTGCTGACAATCTCTTATTCGAATCCGGGGTCATTGATTTCGCAGGTTCCGGCGTTGTTCACTTGGTTGGTGGCATTGCTGGCTTGTGGGGTGCCCTAATTGAAGGCCCCCGCATTGGACGTTTTGATCACGAAGGCAAGTCAGTGGCACTGCGTGGACACAGCGGCACATTGGTTGTTTTGGGCACATTTCTTCTCTGGTTTGGTTGGTATGGCTTCAATGCTGGTTCTTTCCTCAACATTCTAAAGGCATACGGTGCAAGCGGATCATATTACGGGCAATGGAGTGCAATTGGAAGAACGGCGGTCACAACCACACTCGCTGGATGCTCAGCAGCACTCACTACTCTCTTCGGAAAGCGCTTGTTCTCCGGTCATTGGAACGTCACTGATGTCTGCAACGGTTTGCTTGGTGGGTTTGCAGCAATCACTTCTGGCTGCTCGGTTGTCGATCCTTGGGCTGCAATCATTTGCGGGTTTGTGGCGGCTTGGGTTTTGATTGGGTGCAACAAGCTTGCTGAGAAGTTGAAGTATGATGATCCGCTTGAAGCAGCACAGCTCCATGGGGGGTGTGGCTCGTGGGGGATTATTTTCACGGCATTGTTTGCGAAAAAGGCTTATGTGAATGAGATATATGCAGGGCAGCCAAACAGGCCGTATGGGCTTCTGATGGGCGGTGGCGGAAAACTACTGGCGGCGCATTTGGTCCAGATTGTGGTGGTTATAGGGTTTGTGAGTGTGACAATGGGGACATTGTTTTTCTTGCTTCACAAGTTAAAGCTCTTGAGGATTTCAACTGAGGAGGAGATGGCAGGAATGGATGTCACAAGTCATGGTGGATTGGCTTATGTGTATAACGATGAATTCGGCCATCCGGCAACACCGAAACCGGGCTCAAACACAGGCACTACTCAATCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008519 GO:0009506 GO:0015075 GO:0015291 GO:0015398 GO:0015695 GO:0015696 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0072488 GO:0098655

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ammonium_transp

Protein Analysis

498

Amino Acids

52.83

Weight (kDa)

6.82

Isoelectric Point (pI)

20.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ammonium_transp	PF00909	51 - 473	1.2e-130	Ammonium Transporter Family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016519)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28700
fragaria_vesca	FvH4_2g20250
malus_domestica	MD05G1186200.v1.1
prunus_persica	Prupe.1G573000_v2.0.a1 Prupe.8G213000_v2.0.a1
pyrus_communis	pycom05g17070
rosa_chinensis	RchiOBHm_Chr6g0286021
rosa_laevigata	RLG00000012589
rosa_roxburghii	Rroxscaffold_7G00182520
rosa_rugosa	Rorug06G0178300
rosa_samantha	Rh6AG290300 Rh6CG293300 Rh6DG285400
rosa_wichuraiana	Rw6G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	211
Acc36I	ACCTGC	1 cut(s)	605
AccB1I	GGYRCC	1 cut(s)	662
AccB7I	CCANNNNNTGG	1 cut(s)	1285
AccI	GTMKAC	2 cut(s)	123, 516
AccII	CGCG	3 cut(s)	225, 227, 479
AclWI	GGATC	5 cut(s)	223, 446, 844, 1016, 1103
AcoI	YGGCCR	2 cut(s)	91, 1438
AcsI	RAATTY	1 cut(s)	1433
AcuI	CTGAAG	1 cut(s)	570
AcvI	CACGTG	1 cut(s)	572
AdeI	CACNNNGTG	1 cut(s)	725
AfaI	GTAC	1 cut(s)	482
AfeI	AGCGCT	1 cut(s)	932
AfiI	CCNNNNNNNGG	6 cut(s)	47, 332, 686, 948, 1285, 1460
AgsI	TTSAA	5 cut(s)	674, 790, 1099, 1118, 1365
AluBI	AGCT	6 cut(s)	56, 190, 431, 1084, 1129, 1351
AluI	AGCT	6 cut(s)	56, 190, 431, 1084, 1129, 1351
AlwI	GGATC	5 cut(s)	223, 446, 844, 1016, 1103
AlwNI	CAGNNNCTG	1 cut(s)	434
Aor51HI	AGCGCT	1 cut(s)	932
AoxI	GGCC	6 cut(s)	91, 312, 358, 676, 1223, 1438
ApoI	RAATTY	1 cut(s)	1433
ArsI	GACNNNNNNTTYG	2 cut(s)	585, 617
AspLEI	GCGC	4 cut(s)	212, 227, 933, 1270
AspS9I	GGNCC	2 cut(s)	677, 1276
AsuC2I	CCSGG	3 cut(s)	566, 598, 1461
AsuHPI	GGTGA	1 cut(s)	244
AsuII	TTCGAA	1 cut(s)	591
AsuNHI	GCTAGC	1 cut(s)	560
AvaII	GGWCC	1 cut(s)	1276
BaeGI	GKGCMC	2 cut(s)	667, 758
BanI	GGYRCC	1 cut(s)	662
BanII	GRGCYC	1 cut(s)	1466
BauI	CACGAG	1 cut(s)	1147
BbrPI	CACGTG	1 cut(s)	572
BbvCI	CCTCAGC	1 cut(s)	362
BccI	CCATC	4 cut(s)	549, 1234, 1372, 1449
BceAI	ACGGC	3 cut(s)	888, 1182, 1210
BclI	TGATCA	1 cut(s)	697
BcnI	CCSGG	3 cut(s)	566, 598, 1461
BfaI	CTAG	2 cut(s)	191, 561
BfmI	CTRYAG	1 cut(s)	75
BfoI	RGCGCY	1 cut(s)	934
BfuAI	ACCTGC	1 cut(s)	605
BglI	GCCNNNNNGGC	3 cut(s)	71, 1222, 1231
BlpI	GCTNAGC	2 cut(s)	22, 901
Bme1390I	CCNGG	3 cut(s)	566, 598, 1461
Bme18I	GGWCC	1 cut(s)	1276
BmgT120I	GGNCC	2 cut(s)	677, 1276
BmiI	GGNNCC	4 cut(s)	451, 619, 664, 679
BmrFI	CCNGG	3 cut(s)	566, 598, 1461
BmsI	GCATC	3 cut(s)	91, 291, 887
BmtI	GCTAGC	1 cut(s)	564
Bpu10I	CCTNAGC	1 cut(s)	362
Bpu1102I	GCTNAGC	2 cut(s)	22, 901
Bpu14I	TTCGAA	1 cut(s)	591
BpuEI	CTTGAG	1 cut(s)	1375
BpuMI	CCSGG	3 cut(s)	566, 598, 1461
BsaAI	YACGTR	1 cut(s)	572
BsaJI	CCNNGG	4 cut(s)	273, 597, 1025, 1132
BsaWI	WCCGGW	1 cut(s)	941
Bsc4I	CCNNNNNNNGG	6 cut(s)	47, 332, 686, 948, 1285, 1460
Bse1I	ACTGG	2 cut(s)	511, 1266
Bse3DI	GCAATG	5 cut(s)	189, 423, 453, 645, 857
BseDI	CCNNGG	4 cut(s)	273, 597, 1025, 1132
BseGI	GGATG	4 cut(s)	560, 902, 1396, 1441
BseLI	CCNNNNNNNGG	6 cut(s)	47, 332, 686, 948, 1285, 1460
BseMI	GCAATG	5 cut(s)	189, 423, 453, 645, 857
BseMII	CTCAG	5 cut(s)	36, 376, 915, 1080, 1359
BseNI	ACTGG	2 cut(s)	511, 1266
BseRI	GAGGAG	2 cut(s)	1385, 1388
BseSI	GKGCMC	2 cut(s)	667, 758
BseX3I	CGGCCG	1 cut(s)	91
BseYI	CCCAGC	1 cut(s)	434
BsgI	GTGCAG	1 cut(s)	72
Bsh1236I	CGCG	3 cut(s)	225, 227, 479
Bsh1285I	CGRYCG	1 cut(s)	94
BshFI	GGCC	6 cut(s)	93, 314, 360, 678, 1225, 1440
BshNI	GGYRCC	1 cut(s)	662
BsiEI	CGRYCG	1 cut(s)	94
BsiSI	CCGG	6 cut(s)	566, 597, 621, 942, 1445, 1460
BslFI	GGGAC	2 cut(s)	514, 1333
BslI	CCNNNNNNNGG	6 cut(s)	47, 332, 686, 948, 1285, 1460
BsmFI	GGGAC	2 cut(s)	514, 1333
BsnI	GGCC	6 cut(s)	93, 314, 360, 678, 1225, 1440
Bsp119I	TTCGAA	1 cut(s)	591
Bsp1286I	GDGCHC	3 cut(s)	667, 758, 1466
Bsp143I	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
Bsp1720I	GCTNAGC	2 cut(s)	22, 901
Bsp19I	CCATGG	1 cut(s)	1132
BspANI	GGCC	6 cut(s)	93, 314, 360, 678, 1225, 1440
BspCNI	CTCAG	5 cut(s)	35, 375, 914, 1081, 1360
BspFNI	CGCG	3 cut(s)	225, 227, 479
BspLI	GGNNCC	4 cut(s)	451, 619, 664, 679
BspMAI	CTGCAG	1 cut(s)	79
BspMI	ACCTGC	1 cut(s)	605
BspOI	GCTAGC	1 cut(s)	564
BspPI	GGATC	5 cut(s)	223, 446, 844, 1016, 1103
BspT104I	TTCGAA	1 cut(s)	591
BspT107I	GGYRCC	1 cut(s)	662
BsrDI	GCAATG	5 cut(s)	189, 423, 453, 645, 857
BsrI	ACTGG	2 cut(s)	511, 1266
BssECI	CCNNGG	4 cut(s)	273, 597, 1025, 1132
BssMI	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
BssSI	CACGAG	1 cut(s)	1147
BssT1I	CCWWGG	2 cut(s)	1025, 1132
Bst2BI	CACGAG	1 cut(s)	1147
Bst4CI	ACNGT	5 cut(s)	327, 335, 395, 827, 974
Bst6I	CTCTTC	1 cut(s)	926
BstAPI	GCANNNNNTGC	2 cut(s)	827, 1175
BstBAI	YACGTR	1 cut(s)	572
BstBI	TTCGAA	1 cut(s)	591
BstC8I	GCNNGC	4 cut(s)	562, 652, 832, 1086
BstDEI	CTNAG	5 cut(s)	22, 362, 901, 1089, 1368
BstDSI	CCRYGG	2 cut(s)	273, 1132
BstF5I	GGATG	4 cut(s)	560, 902, 1396, 1441
BstFNI	CGCG	3 cut(s)	225, 227, 479
BstH2I	RGCGCY	1 cut(s)	934
BstHHI	GCGC	4 cut(s)	212, 227, 933, 1270
BstKTI	GATC	6 cut(s)	218, 441, 700, 839, 1024, 1111
BstMBI	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
BstMCI	CGRYCG	1 cut(s)	94
BstSCI	CCNGG	3 cut(s)	564, 596, 1459
BstSFI	CTRYAG	1 cut(s)	75
BstSLI	GKGCMC	2 cut(s)	667, 758
BstUI	CGCG	3 cut(s)	225, 227, 479
BstZI	CGGCCG	1 cut(s)	91
BsuRI	GGCC	6 cut(s)	93, 314, 360, 678, 1225, 1440
BtgI	CCRYGG	2 cut(s)	273, 1132
BtsCI	GGATG	4 cut(s)	560, 902, 1396, 1441
BtsI	GCAGTG	2 cut(s)	66, 719
BtsIMutI	CAGTG	4 cut(s)	66, 719, 720, 957
BveI	ACCTGC	1 cut(s)	605
Cac8I	GCNNGC	4 cut(s)	562, 652, 832, 1086
CaiI	CAGNNNCTG	1 cut(s)	434
CciNI	GCGGCCGC	1 cut(s)	91
CfoI	GCGC	4 cut(s)	212, 227, 933, 1270
Cfr13I	GGNCC	2 cut(s)	677, 1276
CseI	GACGC	1 cut(s)	275
Csp6I	GTAC	1 cut(s)	481
CviAII	CATG	4 cut(s)	202, 238, 1133, 1403
CviQI	GTAC	1 cut(s)	481
DdeI	CTNAG	5 cut(s)	22, 362, 901, 1089, 1368
DpnI	GATC	6 cut(s)	217, 440, 699, 838, 1023, 1110
DpnII	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
DraIII	CACNNNGTG	1 cut(s)	725
EaeI	YGGCCR	2 cut(s)	91, 1438
EagI	CGGCCG	1 cut(s)	91
Eam1104I	CTCTTC	1 cut(s)	926
EarI	CTCTTC	1 cut(s)	926
EciI	GGCGGA	1 cut(s)	1266
EclXI	CGGCCG	1 cut(s)	91
Eco130I	CCWWGG	2 cut(s)	1025, 1132
Eco24I	GRGCYC	1 cut(s)	1466
Eco47I	GGWCC	1 cut(s)	1276
Eco47III	AGCGCT	1 cut(s)	932
Eco52I	CGGCCG	1 cut(s)	91
Eco57I	CTGAAG	1 cut(s)	570
Eco72I	CACGTG	1 cut(s)	572
EcoO109I	RGGNCCY	1 cut(s)	677
EcoRI	GAATTC	1 cut(s)	1433
EcoT14I	CCWWGG	2 cut(s)	1025, 1132
EcoT38I	GRGCYC	1 cut(s)	1466
ErhI	CCWWGG	2 cut(s)	1025, 1132
FaeI	CATG	4 cut(s)	205, 241, 1136, 1406
FaqI	GGGAC	2 cut(s)	514, 1333
FatI	CATG	4 cut(s)	201, 237, 1132, 1402
FauI	CCCGC	2 cut(s)	689, 1037
FbaI	TGATCA	1 cut(s)	697
FblI	GTMKAC	2 cut(s)	123, 516
FokI	GGATG	4 cut(s)	567, 909, 1403, 1428
FriOI	GRGCYC	1 cut(s)	1466
FspBI	CTAG	2 cut(s)	191, 561
FspI	TGCGCA	1 cut(s)	211
GlaI	GCGC	4 cut(s)	211, 226, 932, 1269
GsaI	CCCAGC	1 cut(s)	438
HaeII	RGCGCY	1 cut(s)	934
HaeIII	GGCC	6 cut(s)	93, 314, 360, 678, 1225, 1440
HapII	CCGG	6 cut(s)	566, 597, 621, 942, 1445, 1460
HgaI	GACGC	1 cut(s)	275
HhaI	GCGC	4 cut(s)	212, 227, 933, 1270
Hin1II	CATG	4 cut(s)	205, 241, 1136, 1406
Hin6I	GCGC	4 cut(s)	210, 225, 931, 1268
HinP1I	GCGC	4 cut(s)	210, 225, 931, 1268
HincII	GTYRAC	1 cut(s)	124
HindII	GTYRAC	1 cut(s)	124
HindIII	AAGCTT	1 cut(s)	1082
HinfI	GANTC	1 cut(s)	593
HpaII	CCGG	6 cut(s)	566, 597, 621, 942, 1445, 1460
HphI	GGTGA	1 cut(s)	244
Hpy166II	GTNNAC	5 cut(s)	124, 142, 517, 631, 728
Hpy188I	TCNGA	3 cut(s)	38, 926, 1239
Hpy188III	TCNNGA	4 cut(s)	502, 701, 1279, 1354
Hpy8I	GTNNAC	5 cut(s)	124, 142, 517, 631, 728
Hpy99I	CGWCG	1 cut(s)	479
HpyAV	CCTTC	4 cut(s)	508, 545, 668, 698
HpyCH4III	ACNGT	5 cut(s)	327, 335, 395, 827, 974
HpyCH4IV	ACGT	4 cut(s)	258, 571, 691, 954
HpyF3I	CTNAG	5 cut(s)	22, 362, 901, 1089, 1368
HpySE526I	ACGT	4 cut(s)	258, 571, 691, 954
Hsp92II	CATG	4 cut(s)	205, 241, 1136, 1406
HspAI	GCGC	4 cut(s)	210, 225, 931, 1268
Ksp22I	TGATCA	1 cut(s)	697
Kzo9I	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
LmnI	GCTCC	1 cut(s)	1134
LweI	GCATC	3 cut(s)	91, 291, 887
MaeI	CTAG	2 cut(s)	191, 561
MaeII	ACGT	4 cut(s)	258, 571, 691, 954
MaeIII	GTNAC	5 cut(s)	395, 877, 955, 1309, 1393
MalI	GATC	6 cut(s)	217, 440, 699, 838, 1023, 1110
MboI	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
MboII	GAAGA	6 cut(s)	146, 277, 346, 755, 879, 913
MfeI	CAATTG	2 cut(s)	522, 861
MhlI	GDGCHC	3 cut(s)	667, 758, 1466
MluCI	AATT	5 cut(s)	470, 522, 669, 861, 1433
MmeI	TCCRAC	1 cut(s)	61
MnlI	CCTC	8 cut(s)	44, 305, 371, 385, 815, 1350, 1363, 1366
MseI	TTAA	1 cut(s)	1346
MspA1I	CMGCKG	3 cut(s)	56, 431, 735
MspI	CCGG	6 cut(s)	566, 597, 621, 942, 1445, 1460
MspR9I	CCNGG	3 cut(s)	566, 598, 1461
MunI	CAATTG	2 cut(s)	522, 861
MvnI	CGCG	3 cut(s)	225, 227, 479
NciI	CCSGG	3 cut(s)	566, 598, 1461
NcoI	CCATGG	1 cut(s)	1132
NdeII	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
NheI	GCTAGC	1 cut(s)	560
NlaIII	CATG	4 cut(s)	205, 241, 1136, 1406
NlaIV	GGNNCC	4 cut(s)	451, 619, 664, 679
NmuCI	GTSAC	4 cut(s)	877, 955, 1309, 1393
NotI	GCGGCCGC	1 cut(s)	91
NsbI	TGCGCA	1 cut(s)	211
NspV	TTCGAA	1 cut(s)	591
PfeI	GAWTC	1 cut(s)	593
PflMI	CCANNNNNTGG	1 cut(s)	1285
PmaCI	CACGTG	1 cut(s)	572
PmlI	CACGTG	1 cut(s)	572
Ppu21I	YACGTR	1 cut(s)	572
PspCI	CACGTG	1 cut(s)	572
PspFI	CCCAGC	1 cut(s)	434
PspN4I	GGNNCC	4 cut(s)	451, 619, 664, 679
PspPI	GGNCC	2 cut(s)	677, 1276
PstI	CTGCAG	1 cut(s)	79
PstNI	CAGNNNCTG	1 cut(s)	434
PvuII	CAGCTG	2 cut(s)	56, 431
RsaI	GTAC	1 cut(s)	482
RsaNI	GTAC	1 cut(s)	481
SalI	GTCGAC	1 cut(s)	122
SaqAI	TTAA	1 cut(s)	1346
Sau3AI	GATC	6 cut(s)	215, 438, 697, 836, 1021, 1108
Sau96I	GGNCC	2 cut(s)	677, 1276
ScrFI	CCNGG	3 cut(s)	566, 598, 1461
SduI	GDGCHC	3 cut(s)	667, 758, 1466
SfaNI	GCATC	3 cut(s)	91, 291, 887
SfcI	CTRYAG	1 cut(s)	75
SfuI	TTCGAA	1 cut(s)	591
SinI	GGWCC	1 cut(s)	1276
SmlI	CTYRAG	1 cut(s)	1354
SmoI	CTYRAG	1 cut(s)	1354
Sse9I	AATT	5 cut(s)	470, 522, 669, 861, 1433
SspMI	CTAG	2 cut(s)	191, 561
StyD4I	CCNGG	3 cut(s)	564, 596, 1459
StyI	CCWWGG	2 cut(s)	1025, 1132
TaaI	ACNGT	5 cut(s)	327, 335, 395, 827, 974
TaiI	ACGT	4 cut(s)	261, 574, 694, 957
TaqI	TCGA	3 cut(s)	123, 591, 1020
TasI	AATT	5 cut(s)	470, 522, 669, 861, 1433
TauI	GCSGC	8 cut(s)	8, 74, 93, 96, 282, 738, 1058, 1268
TfiI	GAWTC	1 cut(s)	593
Tru1I	TTAA	1 cut(s)	1346
Tru9I	TTAA	1 cut(s)	1346
TscAI	CASTG	4 cut(s)	73, 720, 726, 964
TseFI	GTSAC	4 cut(s)	877, 955, 1309, 1393
Tsp45I	GTSAC	4 cut(s)	877, 955, 1309, 1393
TspDTI	ATGAA	3 cut(s)	254, 328, 1446
TspRI	CASTG	4 cut(s)	73, 720, 726, 964
Van91I	CCANNNNNTGG	1 cut(s)	1285
VpaK11BI	GGWCC	1 cut(s)	1276
XapI	RAATTY	1 cut(s)	1433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1139
XmiI	GTMKAC	2 cut(s)	123, 516
XspI	CTAG	2 cut(s)	191, 561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.