pycom05g17070

Ammonium transporter 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016519
Physical Location & Seq
Reverse (-)
20330049 .. 20331542
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17070.1

Sequence Viewer

Length: 1494 bp
ATGGCGGCTGCTATTTCTTGCTCAGCCACCGACCTCCGACCCCTTTTGGGCGCAGCTGCCAACGCGAGTGCAGCTGCAGAATACATCTGCAGCCGCTTTGATGCTGTGTCAAACAAGTTTGTCGACACAGCCTATGCTGTGGACAACACCTATCTTCTGTTCTCTGCCTACCTTGTTTTTGCAATGCAGTTTGGGTTTGCCATGCTCTGCGCAGGATCAGTTCGCGCGAAAAACACCATGAACATTATGCTCACCAACGTCCTTGACGCTGCAACGAGCAGCATCTTCTACTACCTCTTTGGATTTGCATTGGCCTTTGGCAAACCGTCAAACGGTTTCATAGGGCAGCACTTCTTCGGCCTCAACAAGTTTCCCTCTCAATCTTTCGATTACGGTTACTTCCTTTATCAATGGTCTTTTGCCATTGCAGCTGCTGGGATCACAAGTGGTTCCATTGCTGAACGAACCCAATTCGTCGCGTACTTGATCTATTCGTCCTTCCTGACTGGATTTGTCTACCCAATTGTGTCCCATTGGTTCTGGTCTGCCGACGGATGGGCTAGCCCGACACGTGCTGACAATCTCTTATTCGGGTCTGGGGTCATTGATTTCGCAGGCTCCGGCGTTGTTCACTTGGTTGGTGGCATTGCTGGCTTGTGGGGTGCCCTAATTGAAGGCCCCCGCATTGGACGTTTTGATCAAGAAGGCAGGTCCGTGGCAATGCGTGGACACAACGGCACATTGGTTGTTTTGGGCACATTTCTTCTCTGGTTTGGTTGGTATGGCTTCAATGCTGGCTCTTTCCTCAACATCCTAAAGGCATACAGAACAAGCGGATCATATTACGGGCAATGGAGTGCAATTGGAAGAACTGCGGTCACAACCACACTCGCTGGATGCTCAGCAGCACTCACTACTCTCTTCGGAAAGCGCTTGCTCTCCAGTCATTGGAACGTTACTGATGTCTGCAAGGGTTTGCTTGGTGGGTTTGCAGCAATCACTTCTGGCTGCTCGGTTGTCGATCCTTGGGCTGCAATCATTTGCGGGTTTGTGGTAGCTTGGGTTTTGATTGGGTACAACAAGCTTGCTGAGAAGTTGAAGTATGATGATCCGCTTGAAGCAGCACAGCTCCATGGGGGGTGTGGCTCGTGGGGGATTATTTTCACGGCATTGTTTGCAAAAAAGGCTTATGTGAATGAGATATATGCAGGGCAGCCAAACAGGCCGTATGGGCTTCTGATGGGCGGTGGCGGAAAACTACTGGCGGCGCATTTGGTCCAGATTGTGGTGATTATAGGGTTTGTGAGCGTGACAATGGGGACATTGTTTTTCTTGCTTCACAAGTTAAAGCTCTTGAGGATTTCAACTGAGGTGGAGATGGCAGGAATGGATGTCACAAGTCATGGTGGATTGGCTTATGTGTATGACGATGAATTCAGCTATCCGGCAACACCAAAACTGGGCTCAAACACAGGCACTACTCAATCTGTTTGA

Protein Analysis

498

Amino Acids

53.3

Weight (kDa)

8.29

Isoelectric Point (pI)

21.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 51 - 473 1.7e-128 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28700
fragaria_vesca FvH4_2g20250
malus_domestica MD05G1186200.v1.1
prunus_persica Prupe.1G573000_v2.0.a1 Prupe.8G213000_v2.0.a1
pyrus_communis pycom05g17070
rosa_chinensis RchiOBHm_Chr6g0286021
rosa_laevigata RLG00000012589
rosa_roxburghii Rroxscaffold_7G00182520
rosa_rugosa Rorug06G0178300
rosa_samantha Rh6AG290300 Rh6CG293300 Rh6DG285400
rosa_wichuraiana Rw6G025020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 211
Acc36I ACCTGC 1 cut(s) 699
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 2 cut(s) 948, 1285
AccI GTMKAC 2 cut(s) 123, 516
AccII CGCG 4 cut(s) 65, 225, 227, 479
AclI AACGTT 1 cut(s) 954
AclWI GGATC 5 cut(s) 223, 446, 844, 1016, 1103
AcsI RAATTY 1 cut(s) 1433
AcvI CACGTG 1 cut(s) 572
AfaI GTAC 2 cut(s) 482, 1076
AfeI AGCGCT 1 cut(s) 932
AfiI CCNNNNNNNGG 7 cut(s) 47, 332, 555, 686, 948, 1285, 1460
AflIII ACRYGT 1 cut(s) 569
AgsI TTSAA 5 cut(s) 674, 790, 1099, 1118, 1365
AluBI AGCT 8 cut(s) 56, 74, 431, 1058, 1084, 1129, 1351, 1440
AluI AGCT 8 cut(s) 56, 74, 431, 1058, 1084, 1129, 1351, 1440
AlwI GGATC 5 cut(s) 223, 446, 844, 1016, 1103
AlwNI CAGNNNCTG 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 932
AoxI GGCC 4 cut(s) 312, 358, 676, 1223
ApoI RAATTY 1 cut(s) 1433
AspLEI GCGC 5 cut(s) 53, 212, 227, 933, 1270
AspS9I GGNCC 3 cut(s) 677, 711, 1276
AsuHPI GGTGA 2 cut(s) 244, 1300
AsuNHI GCTAGC 1 cut(s) 560
AvaII GGWCC 2 cut(s) 711, 1276
BaeGI GKGCMC 2 cut(s) 667, 758
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 1466
BauI CACGAG 1 cut(s) 1147
BbrPI CACGTG 1 cut(s) 572
BccI CCATC 3 cut(s) 549, 1234, 1372
BceAI ACGGC 3 cut(s) 751, 1182, 1210
BclI TGATCA 1 cut(s) 697
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 2 cut(s) 75, 88
BfoI RGCGCY 1 cut(s) 934
BfuAI ACCTGC 1 cut(s) 699
BglI GCCNNNNNGGC 2 cut(s) 1222, 1231
BlpI GCTNAGC 2 cut(s) 22, 901
Bme18I GGWCC 2 cut(s) 711, 1276
BmgT120I GGNCC 3 cut(s) 677, 711, 1276
BmiI GGNNCC 4 cut(s) 451, 619, 664, 679
BmrI ACTGGG 1 cut(s) 1469
BmsI GCATC 3 cut(s) 91, 291, 887
BmtI GCTAGC 1 cut(s) 564
BmuI ACTGGG 1 cut(s) 1469
BpmI CTGGAG 1 cut(s) 925
Bpu1102I GCTNAGC 2 cut(s) 22, 901
BpuEI CTTGAG 1 cut(s) 1375
BsaAI YACGTR 1 cut(s) 572
BsaJI CCNNGG 3 cut(s) 714, 1025, 1132
Bsc4I CCNNNNNNNGG 7 cut(s) 47, 332, 555, 686, 948, 1285, 1460
Bse1I ACTGG 4 cut(s) 511, 942, 1266, 1464
Bse3DI GCAATG 6 cut(s) 189, 423, 453, 645, 726, 857
BseDI CCNNGG 3 cut(s) 714, 1025, 1132
BseGI GGATG 4 cut(s) 560, 810, 902, 1396
BseLI CCNNNNNNNGG 7 cut(s) 47, 332, 555, 686, 948, 1285, 1460
BseMI GCAATG 6 cut(s) 189, 423, 453, 645, 726, 857
BseMII CTCAG 4 cut(s) 36, 915, 1080, 1359
BseNI ACTGG 4 cut(s) 511, 942, 1266, 1464
BseSI GKGCMC 2 cut(s) 667, 758
BseYI CCCAGC 1 cut(s) 434
BsgI GTGCAG 1 cut(s) 90
Bsh1236I CGCG 4 cut(s) 65, 225, 227, 479
BshFI GGCC 4 cut(s) 314, 360, 678, 1225
BshNI GGYRCC 1 cut(s) 662
BsiSI CCGG 2 cut(s) 621, 1445
BslFI GGGAC 2 cut(s) 514, 1333
BslI CCNNNNNNNGG 7 cut(s) 47, 332, 555, 686, 948, 1285, 1460
BsmFI GGGAC 2 cut(s) 514, 1333
BsnI GGCC 4 cut(s) 314, 360, 678, 1225
Bsp1286I GDGCHC 3 cut(s) 667, 758, 1466
Bsp143I GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
Bsp1720I GCTNAGC 2 cut(s) 22, 901
Bsp19I CCATGG 1 cut(s) 1132
BspANI GGCC 4 cut(s) 314, 360, 678, 1225
BspCNI CTCAG 4 cut(s) 35, 914, 1081, 1360
BspFNI CGCG 4 cut(s) 65, 225, 227, 479
BspLI GGNNCC 4 cut(s) 451, 619, 664, 679
BspMAI CTGCAG 2 cut(s) 79, 92
BspMI ACCTGC 1 cut(s) 699
BspOI GCTAGC 1 cut(s) 564
BspPI GGATC 5 cut(s) 223, 446, 844, 1016, 1103
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 6 cut(s) 189, 423, 453, 645, 726, 857
BsrI ACTGG 4 cut(s) 511, 942, 1266, 1464
BssECI CCNNGG 3 cut(s) 714, 1025, 1132
BssMI GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
BssSI CACGAG 1 cut(s) 1147
BssT1I CCWWGG 2 cut(s) 1025, 1132
Bst2BI CACGAG 1 cut(s) 1147
Bst4CI ACNGT 3 cut(s) 327, 335, 395
Bst6I CTCTTC 1 cut(s) 926
BstAPI GCANNNNNTGC 1 cut(s) 1175
BstBAI YACGTR 1 cut(s) 572
BstC8I GCNNGC 6 cut(s) 562, 616, 652, 796, 935, 1086
BstDEI CTNAG 4 cut(s) 22, 901, 1089, 1368
BstDSI CCRYGG 2 cut(s) 714, 1132
BstF5I GGATG 4 cut(s) 560, 810, 902, 1396
BstFNI CGCG 4 cut(s) 65, 225, 227, 479
BstH2I RGCGCY 1 cut(s) 934
BstHHI GCGC 5 cut(s) 53, 212, 227, 933, 1270
BstKTI GATC 7 cut(s) 218, 441, 489, 700, 839, 1024, 1111
BstMBI GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
BstMWI GCNNNNNNNGC 8 cut(s) 62, 71, 428, 651, 1175, 1184, 1222, 1231
BstSFI CTRYAG 2 cut(s) 75, 88
BstSLI GKGCMC 2 cut(s) 667, 758
BstUI CGCG 4 cut(s) 65, 225, 227, 479
BsuRI GGCC 4 cut(s) 314, 360, 678, 1225
BtgI CCRYGG 2 cut(s) 714, 1132
BtsCI GGATG 4 cut(s) 560, 810, 902, 1396
BveI ACCTGC 1 cut(s) 699
Cac8I GCNNGC 6 cut(s) 562, 616, 652, 796, 935, 1086
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 5 cut(s) 53, 212, 227, 933, 1270
Cfr13I GGNCC 3 cut(s) 677, 711, 1276
CseI GACGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 481, 1075
CspCI CAANNNNNGTGG 2 cut(s) 1353, 1388
CviAII CATG 4 cut(s) 202, 238, 1133, 1403
CviQI GTAC 2 cut(s) 481, 1075
DdeI CTNAG 4 cut(s) 22, 901, 1089, 1368
DpnI GATC 7 cut(s) 217, 440, 488, 699, 838, 1023, 1110
DpnII GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
Eam1104I CTCTTC 1 cut(s) 926
EarI CTCTTC 1 cut(s) 926
EciI GGCGGA 1 cut(s) 1266
Eco130I CCWWGG 2 cut(s) 1025, 1132
Eco24I GRGCYC 1 cut(s) 1466
Eco47I GGWCC 2 cut(s) 711, 1276
Eco47III AGCGCT 1 cut(s) 932
Eco72I CACGTG 1 cut(s) 572
EcoO109I RGGNCCY 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 1433
EcoT14I CCWWGG 2 cut(s) 1025, 1132
EcoT38I GRGCYC 1 cut(s) 1466
ErhI CCWWGG 2 cut(s) 1025, 1132
FaeI CATG 4 cut(s) 205, 241, 1136, 1406
FaqI GGGAC 2 cut(s) 514, 1333
FatI CATG 4 cut(s) 201, 237, 1132, 1402
FauI CCCGC 2 cut(s) 689, 1037
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 2 cut(s) 123, 516
FokI GGATG 4 cut(s) 567, 797, 909, 1403
FriOI GRGCYC 1 cut(s) 1466
FspBI CTAG 1 cut(s) 561
FspI TGCGCA 1 cut(s) 211
GlaI GCGC 5 cut(s) 52, 211, 226, 932, 1269
GsaI CCCAGC 1 cut(s) 438
GsuI CTGGAG 1 cut(s) 925
HaeII RGCGCY 1 cut(s) 934
HaeIII GGCC 4 cut(s) 314, 360, 678, 1225
HapII CCGG 2 cut(s) 621, 1445
HgaI GACGC 1 cut(s) 275
HhaI GCGC 5 cut(s) 53, 212, 227, 933, 1270
Hin1II CATG 4 cut(s) 205, 241, 1136, 1406
Hin6I GCGC 5 cut(s) 51, 210, 225, 931, 1268
HinP1I GCGC 5 cut(s) 51, 210, 225, 931, 1268
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 1082
HpaII CCGG 2 cut(s) 621, 1445
HphI GGTGA 2 cut(s) 244, 1300
Hpy166II GTNNAC 5 cut(s) 124, 142, 517, 631, 728
Hpy188I TCNGA 3 cut(s) 38, 926, 1239
Hpy188III TCNNGA 4 cut(s) 502, 701, 1279, 1354
Hpy8I GTNNAC 5 cut(s) 124, 142, 517, 631, 728
Hpy99I CGWCG 2 cut(s) 479, 554
HpyAV CCTTC 3 cut(s) 508, 668, 698
HpyCH4III ACNGT 3 cut(s) 327, 335, 395
HpyCH4IV ACGT 4 cut(s) 258, 571, 691, 954
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 71, 428, 651, 1175, 1184, 1222, 1231
HpyF3I CTNAG 4 cut(s) 22, 901, 1089, 1368
HpySE526I ACGT 4 cut(s) 258, 571, 691, 954
Hsp92II CATG 4 cut(s) 205, 241, 1136, 1406
HspAI GCGC 5 cut(s) 51, 210, 225, 931, 1268
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
LmnI GCTCC 2 cut(s) 623, 1134
LweI GCATC 3 cut(s) 91, 291, 887
MaeI CTAG 1 cut(s) 561
MaeII ACGT 4 cut(s) 258, 571, 691, 954
MaeIII GTNAC 5 cut(s) 395, 877, 955, 1309, 1393
MalI GATC 7 cut(s) 217, 440, 488, 699, 838, 1023, 1110
MboI GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
MboII GAAGA 6 cut(s) 146, 277, 346, 755, 879, 913
MfeI CAATTG 2 cut(s) 522, 861
MhlI GDGCHC 3 cut(s) 667, 758, 1466
MluCI AATT 5 cut(s) 470, 522, 669, 861, 1433
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 7 cut(s) 44, 305, 371, 385, 815, 1350, 1363
MseI TTAA 1 cut(s) 1346
MspA1I CMGCKG 3 cut(s) 56, 74, 431
MspI CCGG 2 cut(s) 621, 1445
MunI CAATTG 2 cut(s) 522, 861
MvnI CGCG 4 cut(s) 65, 225, 227, 479
MwoI GCNNNNNNNGC 8 cut(s) 62, 71, 428, 651, 1175, 1184, 1222, 1231
NcoI CCATGG 1 cut(s) 1132
NdeII GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
NheI GCTAGC 1 cut(s) 560
NlaIII CATG 4 cut(s) 205, 241, 1136, 1406
NlaIV GGNNCC 4 cut(s) 451, 619, 664, 679
NmuCI GTSAC 3 cut(s) 877, 1309, 1393
NsbI TGCGCA 1 cut(s) 211
PflMI CCANNNNNTGG 2 cut(s) 948, 1285
PmaCI CACGTG 1 cut(s) 572
PmlI CACGTG 1 cut(s) 572
Ppu21I YACGTR 1 cut(s) 572
Psp1406I AACGTT 1 cut(s) 954
PspCI CACGTG 1 cut(s) 572
PspFI CCCAGC 1 cut(s) 434
PspN4I GGNNCC 4 cut(s) 451, 619, 664, 679
PspPI GGNCC 3 cut(s) 677, 711, 1276
PstI CTGCAG 2 cut(s) 79, 92
PstNI CAGNNNCTG 1 cut(s) 434
PvuII CAGCTG 3 cut(s) 56, 74, 431
RsaI GTAC 2 cut(s) 482, 1076
RsaNI GTAC 2 cut(s) 481, 1075
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 1346
Sau3AI GATC 7 cut(s) 215, 438, 486, 697, 836, 1021, 1108
Sau96I GGNCC 3 cut(s) 677, 711, 1276
SduI GDGCHC 3 cut(s) 667, 758, 1466
SfaNI GCATC 3 cut(s) 91, 291, 887
SfcI CTRYAG 2 cut(s) 75, 88
SinI GGWCC 2 cut(s) 711, 1276
SmlI CTYRAG 1 cut(s) 1354
SmoI CTYRAG 1 cut(s) 1354
Sse9I AATT 5 cut(s) 470, 522, 669, 861, 1433
SspMI CTAG 1 cut(s) 561
StyI CCWWGG 2 cut(s) 1025, 1132
TaaI ACNGT 3 cut(s) 327, 335, 395
TaiI ACGT 4 cut(s) 261, 574, 694, 957
TaqI TCGA 3 cut(s) 123, 387, 1020
TasI AATT 5 cut(s) 470, 522, 669, 861, 1433
TauI GCSGC 3 cut(s) 8, 96, 1268
Tru1I TTAA 1 cut(s) 1346
Tru9I TTAA 1 cut(s) 1346
TseFI GTSAC 3 cut(s) 877, 1309, 1393
Tsp45I GTSAC 3 cut(s) 877, 1309, 1393
TspDTI ATGAA 3 cut(s) 254, 328, 1446
TspGWI ACGGA 2 cut(s) 567, 703
Van91I CCANNNNNTGG 2 cut(s) 948, 1285
VpaK11BI GGWCC 2 cut(s) 711, 1276
XapI RAATTY 1 cut(s) 1433
XcmI CCANNNNNNNNNTGG 1 cut(s) 1139
XmiI GTMKAC 2 cut(s) 123, 516
XspI CTAG 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.