Rorug06G0178300

Ammonium transporter 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016519
Physical Location & Seq
Reverse (-)
28181863 .. 28185044
3182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0178300.1

Sequence Viewer

Length: 783 bp
ATGGCCTTTAAGGAGTTAACCGGAAAGGATTACGATAAAAGCCAACTGAAAAATAAGTGGGATTCACTGAAAAATGATTGGAAATTGTGGAGTTCAGTTATGCATAAGGAAACTGGTATTGGATGGGATCCGGCTAAGAAAACTGTCGACGCACCTGCTGAATGGTGGGAATCCAAAATTCAGATCAATCCAGAGTATCGCAAGTTTTGGGAAGTGGGAATTAGTCCTGAAATGATGGTTGTTTATGACAACATGTTCAGGGGTAGCACAGCCCTAGGTCACTTTGTCATGATTCCCTCGGCTACCATAGATATTGAAGAGGTTGTGGAGGATTCAGAGCATAATTTAATTTCTGGAGATGATGAAGAGGATTCTGACCAAGATAATGAACGTAGAGGCAAAAAGAGAACAAATTTGGAGCGCCAAACAGAGGTTGATAAAGAAAAAAAAGGAAAAGGAGTTATGGGAGGGCCAAAGGGGAAGAAAGAAAAGGTGGGAGGTGCGGCGAAATTGTCTAAACAAATCGATCGTCTTGTTGATGTAGTCGAGAGTAGGAGTACAGCCACATCTGTACGTGATAATGGTACATCACCAGGAACTAGTATTCAAGAGGTGATGAGAGTTGTTGCAACATTACCAGGAGCAGAAACCGGTACCAAGCTATGGTGGTTTGCAACGGAGTTGTTCTGCTCTCAGGAGAAGCGAGAGATGTTTTCTATTATGACAGATGCTGATCTCAAGCTCCAGTTTCTGATTCTTAATAAGAAAAAAGCTGAAAATTAA

Protein Analysis

260

Amino Acids

29.53

Weight (kDa)

5.65

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_3 PF12776 2 - 57 1.4e-10 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28700
fragaria_vesca FvH4_2g20250
malus_domestica MD05G1186200.v1.1
prunus_persica Prupe.1G573000_v2.0.a1 Prupe.8G213000_v2.0.a1
pyrus_communis pycom05g17070
rosa_chinensis RchiOBHm_Chr6g0286021
rosa_laevigata RLG00000012589
rosa_roxburghii Rroxscaffold_7G00182520
rosa_rugosa Rorug06G0178300
rosa_samantha Rh6AG290300 Rh6CG293300 Rh6DG285400
rosa_wichuraiana Rw6G025020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 1 cut(s) 163
Acc65I GGTACC 1 cut(s) 653
AccB1I GGYRCC 1 cut(s) 653
AccB7I CCANNNNNTGG 1 cut(s) 663
AccI GTMKAC 1 cut(s) 147
AciI CCGC 1 cut(s) 503
AclWI GGATC 2 cut(s) 122, 135
AcsI RAATTY 2 cut(s) 177, 412
AfaI GTAC 4 cut(s) 559, 573, 586, 655
AfiI CCNNNNNNNGG 2 cut(s) 430, 663
AflIII ACRYGT 1 cut(s) 252
AgeI ACCGGT 1 cut(s) 650
AgsI TTSAA 2 cut(s) 317, 608
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 2 cut(s) 592, 637
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 3 cut(s) 661, 742, 773
AluI AGCT 3 cut(s) 661, 742, 773
AlwI GGATC 2 cut(s) 122, 135
AlwNI CAGNNNCTG 2 cut(s) 731, 751
AoxI GGCC 2 cut(s) 3, 470
ApoI RAATTY 2 cut(s) 177, 412
ArsI GACNNNNNNTTYG 2 cut(s) 514, 546
AsiGI ACCGGT 1 cut(s) 650
Asp718I GGTACC 1 cut(s) 653
AspA2I CCTAGG 1 cut(s) 274
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 582, 625
AvrII CCTAGG 1 cut(s) 274
BamHI GGATCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 653
BccI CCATC 2 cut(s) 117, 229
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 2 cut(s) 594, 639
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 2 cut(s) 275, 600
BfoI RGCGCY 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 163
BisI GCNGC 1 cut(s) 504
BlnI CCTAGG 1 cut(s) 274
BlsI GCNGC 1 cut(s) 505
Bme1390I CCNGG 2 cut(s) 594, 639
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 2 cut(s) 129, 655
BmrFI CCNGG 2 cut(s) 594, 639
BmsI GCATC 1 cut(s) 718
BpmI CTGGAG 2 cut(s) 375, 728
BpuEI CTTGAG 1 cut(s) 722
Bsa29I ATCGAT 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 732
BsaJI CCNNGG 2 cut(s) 274, 297
BsaWI WCCGGW 2 cut(s) 20, 650
Bsc4I CCNNNNNNNGG 2 cut(s) 430, 663
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 2 cut(s) 118, 745
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 594, 639
BseCI ATCGAT 1 cut(s) 525
BseDI CCNNGG 2 cut(s) 274, 297
BseGI GGATG 1 cut(s) 128
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 430, 663
BseMII CTCAG 1 cut(s) 707
BseNI ACTGG 2 cut(s) 118, 745
Bsh1285I CGRYCG 1 cut(s) 529
BshFI GGCC 2 cut(s) 5, 472
BshNI GGYRCC 1 cut(s) 653
BshTI ACCGGT 1 cut(s) 650
BshVI ATCGAT 1 cut(s) 525
BsiEI CGRYCG 1 cut(s) 529
BsiSI CCGG 3 cut(s) 21, 131, 651
BslI CCNNNNNNNGG 2 cut(s) 430, 663
BsnI GGCC 2 cut(s) 5, 472
Bsp143I GATC 4 cut(s) 127, 183, 526, 733
BspACI CCGC 1 cut(s) 503
BspANI GGCC 2 cut(s) 5, 472
BspCNI CTCAG 1 cut(s) 706
BspDI ATCGAT 1 cut(s) 525
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 129, 655
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 2 cut(s) 122, 135
BspT107I GGYRCC 1 cut(s) 653
BsrFI RCCGGY 1 cut(s) 650
BsrI ACTGG 2 cut(s) 118, 745
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 2 cut(s) 274, 297
BssMI GATC 4 cut(s) 127, 183, 526, 733
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 594, 639
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 312, 360
BstBAI YACGTR 1 cut(s) 575
BstDEI CTNAG 2 cut(s) 135, 693
BstF5I GGATG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 4 cut(s) 130, 186, 529, 736
BstMBI GATC 4 cut(s) 127, 183, 526, 733
BstMCI CGRYCG 1 cut(s) 529
BstNI CCWGG 2 cut(s) 594, 639
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 2 cut(s) 592, 637
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 525
BsuRI GGCC 2 cut(s) 5, 472
BsuTUI ATCGAT 1 cut(s) 525
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 65
BveI ACCTGC 1 cut(s) 163
CaiI CAGNNNCTG 2 cut(s) 731, 751
CciI TCATGA 1 cut(s) 288
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 470
ClaI ATCGAT 1 cut(s) 525
CseI GACGC 1 cut(s) 158
Csp6I GTAC 4 cut(s) 558, 572, 585, 654
CspAI ACCGGT 1 cut(s) 650
CviAII CATG 2 cut(s) 253, 289
CviQI GTAC 4 cut(s) 558, 572, 585, 654
DdeI CTNAG 2 cut(s) 135, 693
DpnI GATC 4 cut(s) 129, 185, 528, 735
DpnII GATC 4 cut(s) 127, 183, 526, 733
Eam1104I CTCTTC 2 cut(s) 312, 360
EarI CTCTTC 2 cut(s) 312, 360
Eco130I CCWWGG 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 592, 637
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 256, 292
FatI CATG 2 cut(s) 252, 288
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 2 cut(s) 275, 600
GlaI GCGC 1 cut(s) 422
GluI GCNGC 1 cut(s) 504
GsuI CTGGAG 2 cut(s) 375, 728
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 2 cut(s) 5, 472
HapII CCGG 3 cut(s) 21, 131, 651
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 423
Hin1II CATG 2 cut(s) 256, 292
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 2 cut(s) 18, 148
HindII GTYRAC 2 cut(s) 18, 148
HinfI GANTC 6 cut(s) 62, 170, 292, 332, 371, 754
HpaI GTTAAC 1 cut(s) 18
HpaII CCGG 3 cut(s) 21, 131, 651
HphI GGTGA 2 cut(s) 582, 625
Hpy166II GTNNAC 2 cut(s) 18, 148
Hpy188I TCNGA 4 cut(s) 183, 337, 376, 753
Hpy188III TCNNGA 7 cut(s) 191, 227, 289, 354, 547, 608, 695
Hpy8I GTNNAC 2 cut(s) 18, 148
Hpy99I CGWCG 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 2 cut(s) 391, 574
HpyCH4V TGCA 3 cut(s) 103, 629, 674
HpyF3I CTNAG 2 cut(s) 135, 693
HpySE526I ACGT 2 cut(s) 391, 574
Hsp92II CATG 2 cut(s) 256, 292
HspAI GCGC 1 cut(s) 421
KpnI GGTACC 1 cut(s) 657
KspAI GTTAAC 1 cut(s) 18
Kzo9I GATC 4 cut(s) 127, 183, 526, 733
LmnI GCTCC 3 cut(s) 418, 641, 747
LweI GCATC 1 cut(s) 718
MaeI CTAG 2 cut(s) 275, 600
MaeII ACGT 2 cut(s) 391, 574
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 129, 185, 528, 735
MboI GATC 4 cut(s) 127, 183, 526, 733
MboII GAAGA 3 cut(s) 329, 377, 493
MflI RGATCY 1 cut(s) 127
MluCI AATT 8 cut(s) 83, 177, 219, 343, 348, 412, 509, 778
MnlI CCTC 9 cut(s) 307, 313, 322, 361, 389, 424, 461, 491, 604
Mph1103I ATGCAT 1 cut(s) 105
MseI TTAA 5 cut(s) 9, 17, 347, 759, 781
MspI CCGG 3 cut(s) 21, 131, 651
MspR9I CCNGG 2 cut(s) 594, 639
MvaI CCWGG 2 cut(s) 594, 639
NdeII GATC 4 cut(s) 127, 183, 526, 733
NlaIII CATG 2 cut(s) 256, 292
NlaIV GGNNCC 2 cut(s) 129, 655
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 105
NspI RCATGY 1 cut(s) 256
PagI TCATGA 1 cut(s) 288
PaqCI CACCTGC 1 cut(s) 163
PciI ACATGT 1 cut(s) 252
PfeI GAWTC 6 cut(s) 62, 170, 292, 332, 371, 754
PflMI CCANNNNNTGG 1 cut(s) 663
PinAI ACCGGT 1 cut(s) 650
PkrI GCNGC 1 cut(s) 505
Ple19I CGATCG 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 575
PscI ACATGT 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 592, 637
PspGI CCWGG 2 cut(s) 592, 637
PspN4I GGNNCC 2 cut(s) 129, 655
PspPI GGNCC 1 cut(s) 470
PstNI CAGNNNCTG 2 cut(s) 731, 751
PsuI RGATCY 1 cut(s) 127
PvuI CGATCG 1 cut(s) 529
RsaI GTAC 4 cut(s) 559, 573, 586, 655
RsaNI GTAC 4 cut(s) 558, 572, 585, 654
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 5 cut(s) 9, 17, 347, 759, 781
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 4 cut(s) 127, 183, 526, 733
Sau96I GGNCC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 594, 639
SfaNI GCATC 1 cut(s) 718
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 8 cut(s) 83, 177, 219, 343, 348, 412, 509, 778
SsiI CCGC 1 cut(s) 503
SspMI CTAG 2 cut(s) 275, 600
StyD4I CCNGG 2 cut(s) 592, 637
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 2 cut(s) 394, 577
TaqI TCGA 3 cut(s) 147, 525, 546
TasI AATT 8 cut(s) 83, 177, 219, 343, 348, 412, 509, 778
TatI WGTACW 1 cut(s) 557
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 6 cut(s) 62, 170, 292, 332, 371, 754
Tru1I TTAA 5 cut(s) 9, 17, 347, 759, 781
Tru9I TTAA 5 cut(s) 9, 17, 347, 759, 781
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 2 cut(s) 378, 402
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 1 cut(s) 72
Van91I CCANNNNNTGG 1 cut(s) 663
XapI RAATTY 2 cut(s) 177, 412
XceI RCATGY 1 cut(s) 256
XmaJI CCTAGG 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 275, 600
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.