RchiOBHm_Chr6g0286021

Ammonium transporter 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016519
Physical Location & Seq
Reverse (-)
49289418 .. 49291313
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25656

Sequence Viewer

Length: 1479 bp
ATGGCGGCCATCACTTGCTCCGCCTCGGACCTCCAGCCCCTCCTGGGCCCCAATGCCACTGCAGCTGCAGAATACATCTGCGGCCGCTTTGGTGCCGTGTCGAACAAGTTTGTCGACACGGGCTATGCAGTTGACAACACGTATCTTCTCTTCTCTGCCTATCTTGTTTTTGCCATGCAGCTCGGCTTTGCAATGCTCTGCGCTGGCTCCGTTCGCGCCAAAAACACCATGAACATTATGCTCACCAATGTCCTCGACGCCGCCACCGGCGGCATCTTCTACTATCTTTTCGGTTTTGCCTTCGCCTTTGGAACTCCCTCCAATGGCTTCATTGGCAAGCAATTCTTTGGTCTCAGCCATTTTCCTTCCCCCTCTTTCGACTACGGCTACTTCCTCTACCAATGGGCCTTTGCCATTGCAGCTGCCGGAATCACTAGTGGTTCGATCGCCGAACGGACCCAATTCGTGGCCTATTTGATCTATTCATCTTTCTTGACCGGATTTGTGTACCCGATTGTGTCACATTGGTTTTGGTCTGCAGATGGATGGGCTAGCCCTGCTCGTGCTGAGAATCTCTTGTTTGGTTCTGGTGTGATTGACTTTGCAGGCTCCGGAGTTGTCCACTTGGTCGGAGGCATTGCCGGTTTGTGGGGTGCCCTGATCGAGGGGCCTAGGATCGGCCGGTTTGATCATGAAGGCCGGTCCGTGGCAATGCGTGGACATAGTGGCACATTGGTTGTTTTGGGCACATTTTTGTTGTGGTTTGGTTGGTATGGTTTCAATGCAGGTTCATTTCTCAACATATTGAAGGTTTATGGTGATAGTGGATCTTACTATGGACAATGGAGTGCTGTGGGCCGGACCGCCGTGACAACCACCTTGGCCGGATGCTCGGCAGCATTGACTACCCTCTTCGGAAAGCGGCTGCTCTCCGGGCACTGGAATGTCACTGATGTGTGCAATGGCTTGCTTGGCGGGTTCGCAGCTATTACATCTGGCTGTTCAGTGGTTGATCCTTGGGCAGCAATTATTTGTGGGATTGTGGCGGCTTGGGTTTTAATCTGGTGCAACAAGCTTGCCGAGAAGTTGAAGTATGATGATCCTTTGGAGGCTGCACAGCTTCATGGTGGGTGTGGTACGTGGGGGATCATATTCACGGCCTTGTTTGCCAAGAAGGCTTATGTGAATGAGGTCTATCCAGGGCACCCGGAGAGGCCATTTGGGCTGTTTATGGGAGGCGGTGCCAAACTGTTAGCTGCGCATTTGGTGCAGATTGTGGTGGTTGCTGTGTTTGTGAGTGTGACGATGGGGACATTGTTTTTCTTGCTGCACAAGTTGAAGCTGTTGAGGATTTCATCAGACGAGGAAATGGCAGGAATGGATGTGACGAGTCATGGAGGATTGGCTTATGTCTATACTGATGAAAGTGGCATGAAATCTGGATATATGGTGTCTAACACTGGTCCTCCACATGTTTGA

Protein Analysis

492

Amino Acids

52.54

Weight (kDa)

6.62

Isoelectric Point (pI)

18.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 48 - 470 2e-128 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28700
fragaria_vesca FvH4_2g20250
malus_domestica MD05G1186200.v1.1
prunus_persica Prupe.1G573000_v2.0.a1 Prupe.8G213000_v2.0.a1
pyrus_communis pycom05g17070
rosa_chinensis RchiOBHm_Chr6g0286021
rosa_laevigata RLG00000012589
rosa_roxburghii Rroxscaffold_7G00182520
rosa_rugosa Rorug06G0178300
rosa_samantha Rh6AG290300 Rh6CG293300 Rh6DG285400
rosa_wichuraiana Rw6G025020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1260
Acc36I ACCTGC 1 cut(s) 776
AccB1I GGYRCC 4 cut(s) 92, 653, 1203, 1241
AccB7I CCANNNNNTGG 1 cut(s) 466
AccI GTMKAC 1 cut(s) 114
AccII CGCG 1 cut(s) 216
AccIII TCCGGA 1 cut(s) 611
AclWI GGATC 5 cut(s) 683, 835, 1007, 1094, 1154
AcoI YGGCCR 4 cut(s) 6, 82, 679, 882
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 2 cut(s) 509, 1138
AfiI CCNNNNNNNGG 8 cut(s) 44, 323, 466, 648, 664, 677, 706, 939
AflIII ACRYGT 2 cut(s) 138, 1471
AgsI TTSAA 4 cut(s) 781, 808, 1090, 1339
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 2 cut(s) 42, 1198
AleI CACNNNNGTG 1 cut(s) 953
AluBI AGCT 8 cut(s) 65, 181, 422, 986, 1075, 1120, 1256, 1342
AluI AGCT 8 cut(s) 65, 181, 422, 986, 1075, 1120, 1256, 1342
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 5 cut(s) 683, 835, 1007, 1094, 1154
Aor13HI TCCGGA 1 cut(s) 611
ApaI GGGCCC 1 cut(s) 50
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 3 cut(s) 203, 218, 1261
AsuC2I CCSGG 2 cut(s) 934, 1208
AsuHPI GGTGA 2 cut(s) 235, 830
AsuNHI GCTAGC 1 cut(s) 551
AvaII GGWCC 5 cut(s) 28, 456, 702, 861, 1463
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 5 cut(s) 50, 658, 749, 939, 1206
BanI GGYRCC 4 cut(s) 92, 653, 1203, 1241
BanII GRGCYC 1 cut(s) 50
BarI GAAGNNNNNNTAC 2 cut(s) 134, 166
BauI CACGAG 1 cut(s) 561
BccI CCATC 4 cut(s) 17, 536, 540, 1300
BceAI ACGGC 4 cut(s) 80, 400, 851, 1173
BciT130I CCWGG 2 cut(s) 44, 1200
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 2 cut(s) 934, 1208
BcoDI GTCTC 1 cut(s) 356
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 3 cut(s) 435, 552, 672
BfmI CTRYAG 3 cut(s) 60, 66, 537
BfuAI ACCTGC 1 cut(s) 776
BglI GCCNNNNNGGC 2 cut(s) 1175, 1222
BlnI CCTAGG 1 cut(s) 671
Bme1390I CCNGG 4 cut(s) 44, 934, 1200, 1208
Bme18I GGWCC 5 cut(s) 28, 456, 702, 861, 1463
BmrFI CCNGG 4 cut(s) 44, 934, 1200, 1208
BmsI GCATC 2 cut(s) 282, 878
BmtI GCTAGC 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 17
BpuMI CCSGG 2 cut(s) 934, 1208
BsaAI YACGTR 2 cut(s) 141, 1140
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 7 cut(s) 24, 43, 671, 705, 879, 1016, 1199
BsaWI WCCGGW 2 cut(s) 497, 611
BsaXI ACNNNNNCTCC 1 cut(s) 1450
Bsc4I CCNNNNNNNGG 8 cut(s) 44, 323, 466, 648, 664, 677, 706, 939
Bse118I RCCGGY 4 cut(s) 266, 641, 681, 699
Bse1I ACTGG 2 cut(s) 944, 1465
Bse3DI GCAATG 5 cut(s) 198, 414, 636, 717, 967
BseAI TCCGGA 1 cut(s) 611
BseBI CCWGG 2 cut(s) 44, 1200
BseDI CCNNGG 7 cut(s) 24, 43, 671, 705, 879, 1016, 1199
BseGI GGATG 3 cut(s) 551, 893, 1387
BseLI CCNNNNNNNGG 8 cut(s) 44, 323, 466, 648, 664, 677, 706, 939
BseMI GCAATG 5 cut(s) 198, 414, 636, 717, 967
BseMII CTCAG 2 cut(s) 367, 558
BseNI ACTGG 2 cut(s) 944, 1465
BseSI GKGCMC 5 cut(s) 50, 658, 749, 939, 1206
BseX3I CGGCCG 2 cut(s) 82, 679
BsgI GTGCAG 3 cut(s) 1098, 1289, 1313
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 3 cut(s) 85, 447, 682
BshNI GGYRCC 4 cut(s) 92, 653, 1203, 1241
BsiEI CGRYCG 3 cut(s) 85, 447, 682
BslFI GGGAC 1 cut(s) 1324
BslI CCNNNNNNNGG 8 cut(s) 44, 323, 466, 648, 664, 677, 706, 939
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 1324
Bso31I GGTCTC 1 cut(s) 356
Bsp120I GGGCCC 1 cut(s) 46
Bsp1286I GDGCHC 5 cut(s) 50, 658, 749, 939, 1206
Bsp13I TCCGGA 1 cut(s) 611
Bsp143I GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
BspCNI CTCAG 2 cut(s) 366, 559
BspEI TCCGGA 1 cut(s) 611
BspFNI CGCG 1 cut(s) 216
BspHI TCATGA 1 cut(s) 691
BspMAI CTGCAG 3 cut(s) 64, 70, 541
BspMI ACCTGC 1 cut(s) 776
BspOI GCTAGC 1 cut(s) 555
BspPI GGATC 5 cut(s) 683, 835, 1007, 1094, 1154
BspT107I GGYRCC 4 cut(s) 92, 653, 1203, 1241
BspTNI GGTCTC 1 cut(s) 356
BsrDI GCAATG 5 cut(s) 198, 414, 636, 717, 967
BsrFI RCCGGY 4 cut(s) 266, 641, 681, 699
BsrI ACTGG 2 cut(s) 944, 1465
BssAI RCCGGY 4 cut(s) 266, 641, 681, 699
BssECI CCNNGG 7 cut(s) 24, 43, 671, 705, 879, 1016, 1199
BssMI GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 3 cut(s) 671, 879, 1016
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 44, 1200
Bst4CI ACNGT 1 cut(s) 1251
Bst6I CTCTTC 2 cut(s) 155, 917
BstACI GRCGYC 1 cut(s) 258
BstAPI GCANNNNNTGC 1 cut(s) 1267
BstBAI YACGTR 2 cut(s) 141, 1140
BstC8I GCNNGC 6 cut(s) 205, 338, 553, 607, 968, 1077
BstDEI CTNAG 2 cut(s) 353, 567
BstDSI CCRYGG 1 cut(s) 705
BstENI CCTNNNNNAGG 1 cut(s) 662
BstF5I GGATG 3 cut(s) 551, 893, 1387
BstFNI CGCG 1 cut(s) 216
BstHHI GCGC 3 cut(s) 203, 218, 1261
BstKTI GATC 9 cut(s) 447, 480, 663, 678, 691, 830, 1015, 1102, 1149
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
BstMCI CGRYCG 3 cut(s) 85, 447, 682
BstNI CCWGG 2 cut(s) 44, 1200
BstNSI RCATGY 1 cut(s) 1475
BstSCI CCNGG 4 cut(s) 42, 932, 1198, 1206
BstSFI CTRYAG 3 cut(s) 60, 66, 537
BstSLI GKGCMC 5 cut(s) 50, 658, 749, 939, 1206
BstUI CGCG 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 827
BstYI RGATCY 1 cut(s) 827
BstZI CGGCCG 2 cut(s) 82, 679
BtgI CCRYGG 1 cut(s) 705
BtsCI GGATG 3 cut(s) 551, 893, 1387
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 5 cut(s) 57, 937, 948, 1011, 1458
BveI ACCTGC 1 cut(s) 776
Cac8I GCNNGC 6 cut(s) 205, 338, 553, 607, 968, 1077
CciI TCATGA 1 cut(s) 691
CciNI GCGGCCGC 1 cut(s) 82
CfoI GCGC 3 cut(s) 203, 218, 1261
Cfr10I RCCGGY 4 cut(s) 266, 641, 681, 699
CpoI CGGWCCG 2 cut(s) 702, 861
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 508, 1137
CspI CGGWCCG 2 cut(s) 702, 861
CviAII CATG 7 cut(s) 175, 229, 692, 1124, 1394, 1432, 1472
CviQI GTAC 2 cut(s) 508, 1137
DdeI CTNAG 2 cut(s) 353, 567
DpnI GATC 9 cut(s) 446, 479, 662, 677, 690, 829, 1014, 1101, 1148
DpnII GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
EaeI YGGCCR 4 cut(s) 6, 82, 679, 882
EagI CGGCCG 2 cut(s) 82, 679
Eam1104I CTCTTC 2 cut(s) 155, 917
EarI CTCTTC 2 cut(s) 155, 917
EciI GGCGGA 1 cut(s) 10
EclXI CGGCCG 2 cut(s) 82, 679
Eco130I CCWWGG 3 cut(s) 671, 879, 1016
Eco24I GRGCYC 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 356
Eco47I GGWCC 5 cut(s) 28, 456, 702, 861, 1463
Eco52I CGGCCG 2 cut(s) 82, 679
EcoNI CCTNNNNNAGG 1 cut(s) 662
EcoO109I RGGNCCY 2 cut(s) 47, 668
EcoRII CCWGG 2 cut(s) 42, 1198
EcoT14I CCWWGG 3 cut(s) 671, 879, 1016
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 3 cut(s) 671, 879, 1016
FaeI CATG 7 cut(s) 178, 232, 695, 1127, 1397, 1435, 1475
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 1 cut(s) 1324
FatI CATG 7 cut(s) 174, 228, 691, 1123, 1393, 1431, 1471
FauI CCCGC 1 cut(s) 968
FbaI TGATCA 1 cut(s) 688
FblI GTMKAC 1 cut(s) 114
FokI GGATG 3 cut(s) 558, 900, 1394
FriOI GRGCYC 1 cut(s) 50
FspBI CTAG 3 cut(s) 435, 552, 672
FspI TGCGCA 1 cut(s) 1260
GlaI GCGC 3 cut(s) 202, 217, 1260
GsuI CTGGAG 1 cut(s) 17
HgaI GACGC 1 cut(s) 266
HhaI GCGC 3 cut(s) 203, 218, 1261
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 7 cut(s) 178, 232, 695, 1127, 1397, 1435, 1475
Hin6I GCGC 3 cut(s) 201, 216, 1259
HinP1I GCGC 3 cut(s) 201, 216, 1259
HincII GTYRAC 2 cut(s) 115, 133
HindII GTYRAC 2 cut(s) 115, 133
HindIII AAGCTT 1 cut(s) 1073
HinfI GANTC 3 cut(s) 429, 571, 1390
HphI GGTGA 2 cut(s) 235, 830
Hpy166II GTNNAC 5 cut(s) 115, 133, 508, 622, 719
Hpy188I TCNGA 4 cut(s) 28, 632, 917, 1360
Hpy188III TCNNGA 4 cut(s) 493, 612, 692, 1440
Hpy8I GTNNAC 5 cut(s) 115, 133, 508, 622, 719
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 5 cut(s) 310, 375, 689, 802, 1168
HpyCH4III ACNGT 1 cut(s) 1251
HpyCH4IV ACGT 2 cut(s) 140, 1139
HpyF3I CTNAG 2 cut(s) 353, 567
HpySE526I ACGT 2 cut(s) 140, 1139
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 7 cut(s) 178, 232, 695, 1127, 1397, 1435, 1475
HspAI GCGC 3 cut(s) 201, 216, 1259
Kpn2I TCCGGA 1 cut(s) 611
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
LmnI GCTCC 3 cut(s) 23, 212, 614
LweI GCATC 2 cut(s) 282, 878
MaeI CTAG 3 cut(s) 435, 552, 672
MaeII ACGT 2 cut(s) 140, 1139
MaeIII GTNAC 5 cut(s) 519, 868, 946, 1300, 1384
MalI GATC 9 cut(s) 446, 479, 662, 677, 690, 829, 1014, 1101, 1148
MboI GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
MboII GAAGA 4 cut(s) 137, 142, 268, 904
MflI RGATCY 1 cut(s) 827
MhlI GDGCHC 5 cut(s) 50, 658, 749, 939, 1206
MluCI AATT 3 cut(s) 341, 461, 1026
MlyI GAGTC 1 cut(s) 1399
MmeI TCCRAC 1 cut(s) 610
MroI TCCGGA 1 cut(s) 611
MseI TTAA 1 cut(s) 1058
MslI CAYNNNNRTG 2 cut(s) 942, 953
MspA1I CMGCKG 2 cut(s) 65, 422
MspR9I CCNGG 4 cut(s) 44, 934, 1200, 1208
MvaI CCWGG 2 cut(s) 44, 1200
MvnI CGCG 1 cut(s) 216
NciI CCSGG 2 cut(s) 934, 1208
NdeII GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
NheI GCTAGC 1 cut(s) 551
NlaIII CATG 7 cut(s) 178, 232, 695, 1127, 1397, 1435, 1475
NmeAIII GCCGAG 3 cut(s) 162, 872, 1105
NmuCI GTSAC 5 cut(s) 519, 868, 946, 1300, 1384
NotI GCGGCCGC 1 cut(s) 82
NsbI TGCGCA 1 cut(s) 1260
NspI RCATGY 1 cut(s) 1475
OliI CACNNNNGTG 1 cut(s) 953
PagI TCATGA 1 cut(s) 691
PciI ACATGT 1 cut(s) 1471
PfeI GAWTC 2 cut(s) 429, 571
PflMI CCANNNNNTGG 1 cut(s) 466
Ple19I CGATCG 1 cut(s) 447
PleI GAGTC 1 cut(s) 1398
PpsI GAGTC 1 cut(s) 1398
Ppu21I YACGTR 2 cut(s) 141, 1140
PscI ACATGT 1 cut(s) 1471
Psp6I CCWGG 2 cut(s) 42, 1198
PspGI CCWGG 2 cut(s) 42, 1198
PspOMI GGGCCC 1 cut(s) 46
PstI CTGCAG 3 cut(s) 64, 70, 541
PsuI RGATCY 1 cut(s) 827
PvuI CGATCG 1 cut(s) 447
PvuII CAGCTG 2 cut(s) 65, 422
RsaI GTAC 2 cut(s) 509, 1138
RsaNI GTAC 2 cut(s) 508, 1137
RseI CAYNNNNRTG 2 cut(s) 942, 953
Rsr2I CGGWCCG 2 cut(s) 702, 861
RsrII CGGWCCG 2 cut(s) 702, 861
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 1058
Sau3AI GATC 9 cut(s) 444, 477, 660, 675, 688, 827, 1012, 1099, 1146
SchI GAGTC 1 cut(s) 1399
ScrFI CCNGG 4 cut(s) 44, 934, 1200, 1208
SduI GDGCHC 5 cut(s) 50, 658, 749, 939, 1206
SfaNI GCATC 2 cut(s) 282, 878
SfcI CTRYAG 3 cut(s) 60, 66, 537
SgrAI CRCCGGYG 1 cut(s) 266
SinI GGWCC 5 cut(s) 28, 456, 702, 861, 1463
SmiMI CAYNNNNRTG 2 cut(s) 942, 953
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 3 cut(s) 341, 461, 1026
SspMI CTAG 3 cut(s) 435, 552, 672
StyD4I CCNGG 4 cut(s) 42, 932, 1198, 1206
StyI CCWWGG 3 cut(s) 671, 879, 1016
TaaI ACNGT 1 cut(s) 1251
TaiI ACGT 2 cut(s) 143, 1142
TaqI TCGA 6 cut(s) 101, 114, 255, 378, 443, 663
TasI AATT 3 cut(s) 341, 461, 1026
TauI GCSGC 7 cut(s) 8, 84, 87, 263, 273, 925, 1049
TfiI GAWTC 2 cut(s) 429, 571
Tru1I TTAA 1 cut(s) 1058
Tru9I TTAA 1 cut(s) 1058
TscAI CASTG 5 cut(s) 64, 944, 955, 1011, 1465
TseFI GTSAC 5 cut(s) 519, 868, 946, 1300, 1384
Tsp45I GTSAC 5 cut(s) 519, 868, 946, 1300, 1384
TspDTI ATGAA 9 cut(s) 245, 319, 474, 708, 780, 1112, 1344, 1437, 1448
TspGWI ACGGA 3 cut(s) 199, 469, 694
TspRI CASTG 5 cut(s) 64, 944, 955, 1011, 1465
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 5 cut(s) 28, 456, 702, 861, 1463
XagI CCTNNNNNAGG 1 cut(s) 662
XceI RCATGY 1 cut(s) 1475
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 3 cut(s) 435, 552, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.