MD05G1219200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017183
Physical Location & Seq
Forward (+)
34967580 .. 34967930
351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1219200.v1.1.491

Sequence Viewer

Length: 351 bp
ATGTCAAAGAGCAAGGACGACATAAAGTATGCGACTTCGCAGGCGAGGCTTGATGAAGACGAAGCTGTGAGGACTGCGTACAAGCATGGGACTCCTCTTGAAGGAGGCAAGATTGCCGAGTCGGAGCCTGTCGATCTCTTCTCCAGCGCTCGTAACATTCCCAAGGCTCAGCCTCGTGATGAAGATTCTAGCAATGATCGATCCCAGATGCAGCAGCATAAACCCGATCTTACGAAACGAGAAGGCGGCCCGGGATCTGCTGCTGATAGTGCTAAATTTCCCAAGAAAACTCCTCCTGCTGATAGTGCTAAATTTCCCAAGAAAACTCCTCCTGCTCTTAGTACTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.6

Weight (kDa)

9.13

Isoelectric Point (pI)

56.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12960
malus_domestica MD05G1219200.v1.1 MD05G1300500.v1.1
prunus_persica Prupe.4G140900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0026701
rosa_laevigata RLG00000032948
rosa_multiflora Rmu_co8063442.1_g000001 Rmu_sc0002301.1_g000002
rosa_rugosa Rorug05G0094900
rosa_samantha Rh5BG184900 Rh5CG204700 Rh5DG187100
rosa_wichuraiana Rw5G017070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 246
AclWI GGATC 2 cut(s) 195, 262
AcsI RAATTY 2 cut(s) 275, 311
AfaI GTAC 2 cut(s) 80, 343
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 101
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
AlwI GGATC 2 cut(s) 195, 262
Ama87I CYCGRG 1 cut(s) 250
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 3 cut(s) 211, 214, 260
ApoI RAATTY 2 cut(s) 275, 311
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 2 cut(s) 251, 252
AvaI CYCGRG 1 cut(s) 250
BauI CACGAG 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 3 cut(s) 223, 226, 247
BcnI CCSGG 2 cut(s) 251, 252
BfaI CTAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 4 cut(s) 212, 215, 247, 261
BlpI GCTNAGC 1 cut(s) 168
BlsI GCNGC 4 cut(s) 213, 216, 248, 262
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 2 cut(s) 251, 252
BmeT110I CYCGRG 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 2 cut(s) 251, 252
BmsI GCATC 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 127
Bpu1102I GCTNAGC 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 251, 252
Bsa29I ATCGAT 1 cut(s) 199
BsaJI CCNNGG 2 cut(s) 162, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 199
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 2 cut(s) 162, 250
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 1 cut(s) 182
BseRI GAGGAG 3 cut(s) 84, 282, 318
BseXI GCAGC 3 cut(s) 223, 226, 247
BshFI GGCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 199
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 1 cut(s) 251
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 103
BsnI GGCC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 250
Bsp143I GATC 5 cut(s) 133, 196, 200, 226, 254
Bsp1720I GCTNAGC 1 cut(s) 168
BspACI CCGC 1 cut(s) 246
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 126
BspPI GGATC 2 cut(s) 195, 262
BsrDI GCAATG 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 162, 250
BssMI GATC 5 cut(s) 133, 196, 200, 226, 254
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 3 cut(s) 168, 338, 345
BstENI CCTNNNNNAGG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 5 cut(s) 136, 199, 203, 229, 257
BstMBI GATC 5 cut(s) 133, 196, 200, 226, 254
BstMWI GCNNNNNNNGC 3 cut(s) 46, 269, 305
BstSCI CCNGG 2 cut(s) 249, 250
BstV1I GCAGC 3 cut(s) 223, 226, 247
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 254
BstYI RGATCY 1 cut(s) 254
Bsu15I ATCGAT 1 cut(s) 199
BsuRI GGCC 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 42
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 248
Cfr9I CCCGGG 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 199
Csp6I GTAC 2 cut(s) 79, 342
CviAII CATG 1 cut(s) 86
CviJI RGCY 6 cut(s) 49, 65, 127, 167, 172, 249
CviKI_1 RGCY 6 cut(s) 49, 65, 127, 167, 172, 249
CviQI GTAC 2 cut(s) 79, 342
DdeI CTNAG 3 cut(s) 168, 338, 345
DpnI GATC 5 cut(s) 135, 198, 202, 228, 256
DpnII GATC 5 cut(s) 133, 196, 200, 226, 254
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 162
Eco47III AGCGCT 1 cut(s) 148
Eco88I CYCGRG 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 1 cut(s) 89
FaiI YATR 4 cut(s) 23, 30, 87, 219
FaqI GGGAC 1 cut(s) 103
FatI CATG 1 cut(s) 85
Fnu4HI GCNGC 4 cut(s) 212, 215, 247, 261
Fsp4HI GCNGC 4 cut(s) 212, 215, 247, 261
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 148
GluI GCNGC 4 cut(s) 212, 215, 247, 261
GsuI CTGGAG 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 1 cut(s) 249
HapII CCGG 1 cut(s) 251
HhaI GCGC 1 cut(s) 149
Hin1II CATG 1 cut(s) 89
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HinfI GANTC 3 cut(s) 91, 119, 185
HpaII CCGG 1 cut(s) 251
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 2 cut(s) 98, 176
HpyAV CCTTC 2 cut(s) 95, 236
HpyCH4V TGCA 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 269, 305
HpyF3I CTNAG 3 cut(s) 168, 338, 345
Hsp92II CATG 1 cut(s) 89
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 5 cut(s) 133, 196, 200, 226, 254
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 7 cut(s) 26, 141, 157, 218, 264, 309, 345
Lsp1109I GCAGC 3 cut(s) 223, 226, 247
LweI GCATC 1 cut(s) 198
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 152
MalI GATC 5 cut(s) 135, 198, 202, 228, 256
MboI GATC 5 cut(s) 133, 196, 200, 226, 254
MboII GAAGA 3 cut(s) 68, 130, 194
MflI RGATCY 1 cut(s) 254
MluCI AATT 2 cut(s) 275, 311
MlyI GAGTC 2 cut(s) 85, 128
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 7 cut(s) 39, 63, 98, 105, 183, 303, 339
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 2 cut(s) 251, 252
MwoI GCNNNNNNNGC 3 cut(s) 46, 269, 305
NciI CCSGG 2 cut(s) 251, 252
NdeII GATC 5 cut(s) 133, 196, 200, 226, 254
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 126
NmeAIII GCCGAG 1 cut(s) 142
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 4 cut(s) 213, 216, 248, 262
PleI GAGTC 2 cut(s) 85, 127
PpsI GAGTC 2 cut(s) 85, 127
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 1 cut(s) 248
PsuI RGATCY 1 cut(s) 254
RsaI GTAC 2 cut(s) 80, 343
RsaNI GTAC 2 cut(s) 79, 342
SatI GCNGC 4 cut(s) 212, 215, 247, 261
Sau3AI GATC 5 cut(s) 133, 196, 200, 226, 254
Sau96I GGNCC 1 cut(s) 248
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 2 cut(s) 85, 128
ScrFI CCNGG 2 cut(s) 251, 252
SetI ASST 1 cut(s) 67
SfaNI GCATC 1 cut(s) 198
SmaI CCCGGG 1 cut(s) 252
Sse9I AATT 2 cut(s) 275, 311
SsiI CCGC 1 cut(s) 246
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 249, 250
StyI CCWWGG 1 cut(s) 162
TaqI TCGA 2 cut(s) 132, 199
TasI AATT 2 cut(s) 275, 311
TatI WGTACW 1 cut(s) 341
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 1 cut(s) 185
TseI GCWGC 3 cut(s) 211, 214, 260
TspDTI ATGAA 2 cut(s) 69, 195
TspMI CCCGGG 1 cut(s) 250
XagI CCTNNNNNAGG 1 cut(s) 99
XapI RAATTY 2 cut(s) 275, 311
XmaI CCCGGG 1 cut(s) 250
XspI CTAG 1 cut(s) 189
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.