Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010434

Physical Location & Seq

Genomic Coordinates Reverse (-)

36310914 .. 36318794

Gene Structure 7881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1229800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGGACCTTCAAATCACATTCAAATCGAACCCACTTCTCGTTTCTTCGACCCCATTCAGTCACACATCTCCAAGACCCCTTCTTTTCAACCTGCCCACAAAGCACAGACCCAAAATTTCCCGGCAAAAACCAACCTTTCGAGTTATGGCGTCTGTAAATTCAAATGGGCCTGATGGGTTTTCTTGGCAGAATCTGACTCGGTCCATACGCCGCGGTTCGGAGCGGTTTTGGTCAGATTTTGGTGAGTCAGTGAAGAAGGAAACTGGGTTTGATTTGAAGGAAGCTAATGTGACGGTAGGTGAGTTTGTTGGGCGAATTGGGGACGGGTTAAAGAAGGGTGGGACTGAGTTGGAGCGGTTTAGGACTGAGTTGCTGCCGGAGTTCGTCAGCTGGAATCGGTGGGAACGTTGGAAGGATCTCAAGACATGGGAATCTAAAAGAGTTGCTGCTTTGATTTTCTATGTTTTTATTACCTTAGTTTCTTGTCAAAGAATATATATAGCAATTCGAGCTCCTCTACAAAATCGTCAAAGAAAAGAGTTGACAGAGGCTTATATGGAGGCAGTGATTCCTGAACCATCTCCAATCAATGTTAGAAGGTTTAAGAAGGGCATGTGGAGGAAGATGACACCCAAAGGCTTGAAGATGAAGAAGTTTGTTGAAGGACCTGATGGAACACTTGTTCATGATAGTTCTTATGTTGGAGAGGATGCATGGGATGATGATCCACAGCCGCCTCAGGACAATGTGAAACAAATTATTGACAGTGATGTGAAATTAAATCCAGAGGAGAAGAAGGAACTGGAGGAAGACTTGGGAATTTCTGGGCAAGTTCAAGAAGATAGTGGAACATGGCGTGAAAGACTTCAAAAATGGAATGTGGTCCTTCAGAAGGAAAAGTTAGCTGAGCAGTTGGACTCTGCAAAATCTAAGTATGTAGTTGAATTTGACATGAAAGAGGTTGAAAACAGTCTTCGCAAAGATGTGGTGGAAAAGGTAACAGAGACACAGGGAACCAGGGCATTATGGATAGCTAAAAGATGGTGGCTATATCGTCCTAGACTTCCATATACGTACTTTCTTCAGAAGCTTGATTGCTCTGAGGTAGCTGCTGTTGTATTTACTGAGGACCTAAAAAGGATATATGTTACCATGAAAGAGGGTTTTCCATTAGAATATGTTGTTGATATCCCCCTAGACCCTTACTTGTTTGAGATTATCTCAAGTTCTGGAGTTGAAGTTGATCTTCTTCAAAAGCGCCAGATCCACTACTTTATGAAAGTTCTAATTGCATTGGTGCCTGGGATACTTATTCTCTGGCTTATACGCGAGTCTGTGATGCTTCTGCACATCACTTCTAAGCGTTTCCTTTACAAAAAGTATAATCAACTTTTTGACATGGCTTATGCGGAAAATTTTATCCTGCCTGTTGGAGATGTGGGGGAAACAAACTCAATGTCCAAGGAAGTTGTGTTAGGAGGTGATGTTTGGGATCTTCTTGACGAGCTGATGGTTTATATGGGAAATCCAATGCAATACTATGAAAGAGAAGTCAAGTTTGTCCGGGGTGTTCTTCTCTCTGGACCTCCTGGAACTGGAAAAACACTTTTTGCGAGGACATTAGCCAAGGAAAGTGGAATGCCATTTGTTTTTGCTTCGGGTGCTGAGTTCACAGATAGTGAAAAAAGTGGTGCTGCAAGAATTAATGAGATGTTTTCGATCGCAAGGAGAAATGCTCCTTCTTTTGTATTTGTGGATGAAATAGATGCTATTGCCGGAAGGCATGCAAGATTGGATCCTCGAAGGAGAGCAACATTTGAGGCTTTGATTGCACAGCTTGATGGGGAGAAGGAAAAAACTGGTGTTGATCGGTTCTCTCTTAGACAAGCTGTGATATTCATCTGTGCTACGAATAGGCCAGATGAGTTAGACCTTGAATTTGTTCGACCTGGGCGTATTGACCGTCGACTGTATATTGGTTTGCCTGATGCAAAGCAACGAGTGCAAATTTTTGGTGTGCACAGTGCTGGAAAGCAACTTGCAGAAGATGTAGACTTTGGAAAACTTGTTTTCCGCACTGTTGGTTTTTCTGGGGCAGATATTCGGAATCTTGTCAATGAAGCAGCAATCATGTCAGTGAGGAAAGGGCGTTCGAGGATCTACCAGGAAGACATTGTTGATGTGTTAGATAAACAATTGCTTGAGGGTATGGGTGTACTTCTTACTGAAGAAGAGCAGCAAAAATGCGAACAAAGTGTGTCTTCGGAAAAGAAGAAACTGCTAGCTGTTCATGAAGCTGGTCACATAGTGTTGGCTCATCTGTTTCCTCAATTTGATTGGCATGCATTTTCTCAGCTTCTGCCTGGTGGAAAGGAAACTGCAATATCTGTGTTTTTCCCCCGAGAAGATATGGTAGACCAAGGTTATACGACCTTTGGCTATATGATGATGCAAATGGTGGTAGCTCATGGTGGGCGTTGTGCTGAGCGTGTTGTGTTTGGTGATGACATAACTGATGGAGGAAGAGATGATTTGGAGAAGATAACAAAGATTGCTCGAGAGATGGTAATCAGCCCTCAAAATTCAAGGTTGGGGCTTACATCTTTAACCAAGAGAGTTGGACTTGTGGATCGACCAGATAGTCCAGATGGCGAGTTGATCAGATACAGGTGGGATGACCCCCATGTGATTCCGGCCAATATGACGCTTGAAGTGTCTGAGCTATTTACCCGAGAATTGACAAGGTACATTGAAGAAACAGAAGAACTCGCAATGAATGGTTTGAGAAACAATAGGCACATATTGGACATGATTATAAAGGAACTATTGGAAAAGTCGAGGATTACTGGTTTGGAGGTTGAAGAGAAAATTAAGGATCTTTCTCCTGTGATGTTTGAGGATTTTGTGAAGCCGTTTCAAATAGACTTGGAAAAGGATGGTCCGTTGCCACACAACGATCAGTTGCGGTATAAACCACTGGACATATATCCTGCACCACTGCATAGATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

1004

Amino Acids

115.16

Weight (kDa)

6.11

Isoelectric Point (pI)

44.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	524 - 660	8.3e-33	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	683 - 725	3.4e-13	AAA+ lid domain
Peptidase_M41	PF01434	753 - 954	2e-23	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79560 AT1G79560 AT1G79560
fragaria_vesca	FvH4_3g14760 FvH4_3g14760 FvH4_3g14760 FvH4_3g14760
malus_domestica	MD05G1229800.v1.1
prunus_persica	Prupe.4G132700_v2.0.a1
pyrus_communis	pycom05g21070
rosa_chinensis	RchiOBHm_Chr5g0024281
rosa_laevigata	RLG00000032790
rosa_multiflora	Rmu_sc0002344.1_g000002
rosa_roxburghii	Rroxscaffold_1G00054900 Rroxscaffold_1G00055780
rosa_rugosa	Rorug05G0083000
rosa_samantha	Rh5AG173500 Rh5CG186500 Rh5DG173000
rosa_wichuraiana	Rw0G000870 Rw5G015670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2816
AasI	GACNNNNNNGTC	1 cut(s)	2640
Acc36I	ACCTGC	1 cut(s)	99
AccB1I	GGYRCC	1 cut(s)	1297
AccBSI	CCGCTC	2 cut(s)	223, 355
AccI	GTMKAC	3 cut(s)	1966, 2052, 2415
AccII	CGCG	2 cut(s)	213, 1329
AciI	CCGC	8 cut(s)	211, 213, 223, 355, 734, 1409, 2074, 2965
AclI	AACGTT	1 cut(s)	406
AclWI	GGATC	9 cut(s)	423, 719, 1258, 1500, 1790, 1803, 2165, 2637, 2883
AcoI	YGGCCR	1 cut(s)	2694
AcsI	RAATTY	8 cut(s)	114, 157, 819, 944, 1414, 1937, 2007, 2581
AcuI	CTGAAG	3 cut(s)	872, 1067, 2247
AcyI	GRCGYC	1 cut(s)	149
AdeI	CACNNNGTG	1 cut(s)	2308
AfaI	GTAC	3 cut(s)	1076, 2217, 2747
AfiI	CCNNNNNNNGG	3 cut(s)	217, 892, 1595
AjnI	CCWGG	6 cut(s)	1016, 1300, 1588, 1948, 2163, 2362
AloI	GAACNNNNNNTCC	4 cut(s)	666, 698, 2134, 2166
Alw21I	GWGCWC	2 cut(s)	514, 2022
Alw26I	GTCTC	1 cut(s)	998
Alw44I	GTGCAC	1 cut(s)	2018
AlwI	GGATC	9 cut(s)	423, 719, 1258, 1500, 1790, 1803, 2165, 2637, 2883
AlwNI	CAGNNNCTG	2 cut(s)	193, 2359
Ama87I	CYCGRG	3 cut(s)	2400, 2556, 2730
AoxI	GGCC	3 cut(s)	167, 1916, 2694
ApaLI	GTGCAC	1 cut(s)	2018
ApeKI	GCWGC	6 cut(s)	373, 446, 1109, 1694, 2123, 2236
ApoI	RAATTY	8 cut(s)	114, 157, 819, 944, 1414, 1937, 2007, 2581
ArsI	GACNNNNNNTTYG	4 cut(s)	1442, 1474, 1922, 1954
AseI	ATTAAT	1 cut(s)	1704
Asp700I	GAANNNNTTC	2 cut(s)	864, 1941
AspLEI	GCGC	1 cut(s)	1260
AspS9I	GGNCC	8 cut(s)	4, 167, 201, 665, 883, 1129, 1583, 2939
AsuC2I	CCSGG	2 cut(s)	121, 1565
AsuHPI	GGTGA	4 cut(s)	254, 311, 1493, 2513
AsuNHI	GCTAGC	1 cut(s)	2281
AvaI	CYCGRG	3 cut(s)	2400, 2556, 2730
AvaII	GGWCC	7 cut(s)	4, 201, 665, 883, 1129, 1583, 2939
AxyI	CCTNAGG	1 cut(s)	738
BaeGI	GKGCMC	1 cut(s)	2022
BamHI	GGATCC	1 cut(s)	1795
BanI	GGYRCC	1 cut(s)	1297
BanII	GRGCYC	1 cut(s)	514
BbsI	GAAGAC	4 cut(s)	816, 965, 2175, 2253
Bbv12I	GWGCWC	2 cut(s)	514, 2022
BbvI	GCAGC	6 cut(s)	360, 433, 1096, 1681, 2135, 2248
BceAI	ACGGC	1 cut(s)	2896
BciT130I	CCWGG	6 cut(s)	1018, 1302, 1590, 1950, 2165, 2364
BciVI	GTATCC	1 cut(s)	1299
BclI	TGATCA	1 cut(s)	2658
BcnI	CCSGG	2 cut(s)	121, 1565
BcoDI	GTCTC	1 cut(s)	998
BfaI	CTAG	3 cut(s)	1059, 1196, 2282
BfoI	RGCGCY	1 cut(s)	1261
BfuAI	ACCTGC	1 cut(s)	99
BfuI	GTATCC	1 cut(s)	1299
BisI	GCNGC	8 cut(s)	211, 374, 447, 734, 1110, 1695, 2124, 2237
BlpI	GCTNAGC	2 cut(s)	906, 2484
BlsI	GCNGC	8 cut(s)	212, 375, 448, 735, 1111, 1696, 2125, 2238
Bme1390I	CCNGG	8 cut(s)	121, 1018, 1302, 1565, 1590, 1950, 2165, 2364
Bme18I	GGWCC	7 cut(s)	4, 201, 665, 883, 1129, 1583, 2939
BmeT110I	CYCGRG	3 cut(s)	2400, 2556, 2730
BmgT120I	GGNCC	8 cut(s)	4, 167, 201, 665, 883, 1129, 1583, 2939
BmiI	GGNNCC	3 cut(s)	1015, 1299, 1797
BmrFI	CCNGG	8 cut(s)	121, 1018, 1302, 1565, 1590, 1950, 2165, 2364
BmrI	ACTGGG	1 cut(s)	273
BmsI	GCATC	5 cut(s)	700, 1329, 1756, 1978, 2439
BmtI	GCTAGC	1 cut(s)	2285
BmuI	ACTGGG	1 cut(s)	273
BpiI	GAAGAC	4 cut(s)	816, 965, 2175, 2253
BplI	GAGNNNNNCTC	4 cut(s)	1205, 1237, 1720, 1752
BpmI	CTGGAG	2 cut(s)	824, 1251
Bpu1102I	GCTNAGC	2 cut(s)	906, 2484
BpuEI	CTTGAG	3 cut(s)	404, 1207, 2222
BpuMI	CCSGG	2 cut(s)	121, 1565
BsaAI	YACGTR	1 cut(s)	1074
BsaBI	GATNNNNATC	3 cut(s)	723, 1898, 2567
BsaHI	GRCGYC	1 cut(s)	149
BsaJI	CCNNGG	8 cut(s)	211, 1017, 1301, 1461, 1564, 1626, 1949, 2419
BsaXI	ACNNNNNCTCC	4 cut(s)	1224, 1254, 1470, 1500
Bsc4I	CCNNNNNNNGG	3 cut(s)	217, 892, 1595
Bse1I	ACTGG	6 cut(s)	268, 807, 1600, 1864, 2851, 2982
Bse21I	CCTNAGG	1 cut(s)	738
Bse3DI	GCAATG	1 cut(s)	2778
Bse8I	GATNNNNATC	3 cut(s)	723, 1898, 2567
BseBI	CCWGG	6 cut(s)	1018, 1302, 1590, 1950, 2165, 2364
BseDI	CCNNGG	8 cut(s)	211, 1017, 1301, 1461, 1564, 1626, 1949, 2419
BseGI	GGATG	5 cut(s)	715, 724, 1762, 2680, 2941
BseJI	GATNNNNATC	3 cut(s)	723, 1898, 2567
BseLI	CCNNNNNNNGG	3 cut(s)	217, 892, 1595
BseMI	GCAATG	1 cut(s)	2778
BseNI	ACTGG	6 cut(s)	268, 807, 1600, 1864, 2851, 2982
BseRI	GAGGAG	2 cut(s)	504, 803
BseSI	GKGCMC	1 cut(s)	2022
BseXI	GCAGC	6 cut(s)	360, 433, 1096, 1681, 2135, 2248
BsgI	GTGCAG	2 cut(s)	1331, 2976
Bsh1236I	CGCG	2 cut(s)	213, 1329
Bsh1285I	CGRYCG	1 cut(s)	1722
BshFI	GGCC	3 cut(s)	169, 1918, 2696
BshNI	GGYRCC	1 cut(s)	1297
BsiEI	CGRYCG	1 cut(s)	1722
BsiHKAI	GWGCWC	2 cut(s)	514, 2022
BsiHKCI	CYCGRG	3 cut(s)	2400, 2556, 2730
BsiSI	CCGG	5 cut(s)	121, 377, 1564, 1776, 2693
BslFI	GGGAC	2 cut(s)	335, 355
BslI	CCNNNNNNNGG	3 cut(s)	217, 892, 1595
BsmAI	GTCTC	1 cut(s)	998
BsmFI	GGGAC	2 cut(s)	335, 355
BsmI	GAATGC	1 cut(s)	1644
BsnI	GGCC	3 cut(s)	169, 1918, 2696
BsoBI	CYCGRG	3 cut(s)	2400, 2556, 2730
Bsp1286I	GDGCHC	2 cut(s)	514, 2022
Bsp1720I	GCTNAGC	2 cut(s)	906, 2484
BspACI	CCGC	8 cut(s)	211, 213, 223, 355, 734, 1409, 2074, 2965
BspANI	GGCC	3 cut(s)	169, 1918, 2696
BspFNI	CGCG	2 cut(s)	213, 1329
BspHI	TCATGA	2 cut(s)	685, 2290
BspLI	GGNNCC	3 cut(s)	1015, 1299, 1797
BspMI	ACCTGC	1 cut(s)	99
BspOI	GCTAGC	1 cut(s)	2285
BspPI	GGATC	9 cut(s)	423, 719, 1258, 1500, 1790, 1803, 2165, 2637, 2883
BspQI	GCTCTTC	1 cut(s)	2226
BspT107I	GGYRCC	1 cut(s)	1297
BsrBI	CCGCTC	2 cut(s)	223, 355
BsrDI	GCAATG	1 cut(s)	2778
BsrI	ACTGG	6 cut(s)	268, 807, 1600, 1864, 2851, 2982
BssECI	CCNNGG	8 cut(s)	211, 1017, 1301, 1461, 1564, 1626, 1949, 2419
BssNI	GRCGYC	1 cut(s)	149
BssT1I	CCWWGG	3 cut(s)	1461, 1626, 2419
Bst2UI	CCWGG	6 cut(s)	1018, 1302, 1590, 1950, 2165, 2364
Bst4CI	ACNGT	7 cut(s)	295, 767, 971, 1964, 1971, 2024, 2080
Bst6I	CTCTTC	3 cut(s)	2226, 2518, 2856
BstACI	GRCGYC	1 cut(s)	149
BstAPI	GCANNNNNTGC	1 cut(s)	2002
BstBAI	YACGTR	1 cut(s)	1074
BstC8I	GCNNGC	3 cut(s)	1785, 2283, 2343
BstDSI	CCRYGG	1 cut(s)	211
BstENI	CCTNNNNNAGG	1 cut(s)	890
BstF5I	GGATG	5 cut(s)	715, 724, 1762, 2680, 2941
BstFNI	CGCG	2 cut(s)	213, 1329
BstH2I	RGCGCY	1 cut(s)	1261
BstHHI	GCGC	1 cut(s)	1260
BstMAI	GTCTC	1 cut(s)	998
BstMCI	CGRYCG	1 cut(s)	1722
BstMWI	GCNNNNNNNGC	5 cut(s)	100, 509, 1661, 1829, 2002
BstNI	CCWGG	6 cut(s)	1018, 1302, 1590, 1950, 2165, 2364
BstNSI	RCATGY	3 cut(s)	616, 1787, 2345
BstSCI	CCNGG	8 cut(s)	119, 1016, 1300, 1563, 1588, 1948, 2163, 2362
BstSLI	GKGCMC	1 cut(s)	2022
BstSNI	TACGTA	1 cut(s)	1074
BstUI	CGCG	2 cut(s)	213, 1329
BstV1I	GCAGC	6 cut(s)	360, 433, 1096, 1681, 2135, 2248
BstV2I	GAAGAC	4 cut(s)	816, 965, 2175, 2253
BstX2I	RGATCY	6 cut(s)	415, 1263, 1492, 1795, 2157, 2875
BstYI	RGATCY	6 cut(s)	415, 1263, 1492, 1795, 2157, 2875
Bsu36I	CCTNAGG	1 cut(s)	738
BsuI	GTATCC	1 cut(s)	1299
BsuRI	GGCC	3 cut(s)	169, 1918, 2696
BtgI	CCRYGG	1 cut(s)	211
BtsCI	GGATG	5 cut(s)	715, 724, 1762, 2680, 2941
BtsI	GCAGTG	2 cut(s)	570, 2996
BtsIMutI	CAGTG	8 cut(s)	255, 570, 772, 2029, 2076, 2142, 2975, 2996
BveI	ACCTGC	1 cut(s)	99
Cac8I	GCNNGC	3 cut(s)	1785, 2283, 2343
CaiI	CAGNNNCTG	2 cut(s)	193, 2359
CciI	TCATGA	2 cut(s)	685, 2290
CfoI	GCGC	1 cut(s)	1260
Cfr13I	GGNCC	8 cut(s)	4, 167, 201, 665, 883, 1129, 1583, 2939
Cfr42I	CCGCGG	1 cut(s)	214
CseI	GACGC	2 cut(s)	138, 2713
Csp6I	GTAC	3 cut(s)	1075, 2216, 2746
CspCI	CAANNNNNGTGG	2 cut(s)	1615, 1650
CviQI	GTAC	3 cut(s)	1075, 2216, 2746
DraIII	CACNNNGTG	1 cut(s)	2308
DrdI	GACNNNNNNGTC	1 cut(s)	2640
DseDI	GACNNNNNNGTC	1 cut(s)	2640
EaeI	YGGCCR	1 cut(s)	2694
Eam1104I	CTCTTC	3 cut(s)	2226, 2518, 2856
EarI	CTCTTC	3 cut(s)	2226, 2518, 2856
Ecl136II	GAGCTC	1 cut(s)	512
Eco105I	TACGTA	1 cut(s)	1074
Eco130I	CCWWGG	3 cut(s)	1461, 1626, 2419
Eco24I	GRGCYC	1 cut(s)	514
Eco32I	GATATC	1 cut(s)	1189
Eco47I	GGWCC	7 cut(s)	4, 201, 665, 883, 1129, 1583, 2939
Eco53kI	GAGCTC	1 cut(s)	512
Eco57I	CTGAAG	3 cut(s)	872, 1067, 2247
Eco81I	CCTNAGG	1 cut(s)	738
Eco88I	CYCGRG	3 cut(s)	2400, 2556, 2730
EcoICRI	GAGCTC	1 cut(s)	512
EcoNI	CCTNNNNNAGG	1 cut(s)	890
EcoO109I	RGGNCCY	2 cut(s)	665, 1129
EcoRII	CCWGG	6 cut(s)	1016, 1300, 1588, 1948, 2163, 2362
EcoRV	GATATC	1 cut(s)	1189
EcoT14I	CCWWGG	3 cut(s)	1461, 1626, 2419
EcoT22I	ATGCAT	2 cut(s)	715, 2347
EcoT38I	GRGCYC	1 cut(s)	514
ErhI	CCWWGG	3 cut(s)	1461, 1626, 2419
FalI	AAGNNNNNCTT	4 cut(s)	1230, 1262, 2245, 2277
FaqI	GGGAC	2 cut(s)	335, 355
FbaI	TGATCA	1 cut(s)	2658
FblI	GTMKAC	3 cut(s)	1966, 2052, 2415
Fnu4HI	GCNGC	8 cut(s)	211, 374, 447, 734, 1110, 1695, 2124, 2237
FokI	GGATG	5 cut(s)	722, 731, 1769, 2687, 2948
FriOI	GRGCYC	1 cut(s)	514
Fsp4HI	GCNGC	8 cut(s)	211, 374, 447, 734, 1110, 1695, 2124, 2237
FspBI	CTAG	3 cut(s)	1059, 1196, 2282
GlaI	GCGC	1 cut(s)	1259
GluI	GCNGC	8 cut(s)	211, 374, 447, 734, 1110, 1695, 2124, 2237
GsuI	CTGGAG	2 cut(s)	824, 1251
HaeII	RGCGCY	1 cut(s)	1261
HaeIII	GGCC	3 cut(s)	169, 1918, 2696
HapII	CCGG	5 cut(s)	121, 377, 1564, 1776, 2693
HgaI	GACGC	2 cut(s)	138, 2713
HhaI	GCGC	1 cut(s)	1260
Hin1I	GRCGYC	1 cut(s)	149
Hin6I	GCGC	1 cut(s)	1258
HinP1I	GCGC	1 cut(s)	1258
HincII	GTYRAC	2 cut(s)	543, 1967
HindII	GTYRAC	2 cut(s)	543, 1967
HindIII	AAGCTT	1 cut(s)	1088
HpaII	CCGG	5 cut(s)	121, 377, 1564, 1776, 2693
HphI	GGTGA	4 cut(s)	254, 311, 1493, 2513
Hpy166II	GTNNAC	7 cut(s)	543, 1671, 1967, 2020, 2053, 2216, 2416
Hpy8I	GTNNAC	7 cut(s)	543, 1671, 1967, 2020, 2053, 2216, 2416
Hpy99I	CGWCG	1 cut(s)	1968
HpyCH4III	ACNGT	7 cut(s)	295, 767, 971, 1964, 1971, 2024, 2080
HpyCH4IV	ACGT	2 cut(s)	406, 1073
HpyF10VI	GCNNNNNNNGC	5 cut(s)	100, 509, 1661, 1829, 2002
HpySE526I	ACGT	2 cut(s)	406, 1073
Hsp92I	GRCGYC	1 cut(s)	149
HspAI	GCGC	1 cut(s)	1258
Ksp22I	TGATCA	1 cut(s)	2658
KspI	CCGCGG	1 cut(s)	214
LguI	GCTCTTC	1 cut(s)	2226
LmnI	GCTCC	4 cut(s)	220, 352, 517, 1741
Lsp1109I	GCAGC	6 cut(s)	360, 433, 1096, 1681, 2135, 2248
LweI	GCATC	5 cut(s)	700, 1329, 1756, 1978, 2439
MaeI	CTAG	3 cut(s)	1059, 1196, 2282
MaeII	ACGT	2 cut(s)	406, 1073
MaeIII	GTNAC	5 cut(s)	59, 289, 997, 1147, 2300
MbiI	CCGCTC	2 cut(s)	223, 355
MfeI	CAATTG	1 cut(s)	2195
MflI	RGATCY	6 cut(s)	415, 1263, 1492, 1795, 2157, 2875
MhlI	GDGCHC	2 cut(s)	514, 2022
MlyI	GAGTC	4 cut(s)	190, 254, 911, 1340
MmeI	TCCRAC	6 cut(s)	330, 389, 682, 894, 1411, 2599
Mph1103I	ATGCAT	2 cut(s)	715, 2347
MroXI	GAANNNNTTC	2 cut(s)	864, 1941
MseI	TTAA	7 cut(s)	329, 603, 779, 1704, 2606, 2871, 3010
MslI	CAYNNNNRTG	1 cut(s)	2135
MspA1I	CMGCKG	2 cut(s)	213, 390
MspI	CCGG	5 cut(s)	121, 377, 1564, 1776, 2693
MspR9I	CCNGG	8 cut(s)	121, 1018, 1302, 1565, 1590, 1950, 2165, 2364
MunI	CAATTG	1 cut(s)	2195
Mva1269I	GAATGC	1 cut(s)	1644
MvaI	CCWGG	6 cut(s)	1018, 1302, 1590, 1950, 2165, 2364
MvnI	CGCG	2 cut(s)	213, 1329
MwoI	GCNNNNNNNGC	5 cut(s)	100, 509, 1661, 1829, 2002
NciI	CCSGG	2 cut(s)	121, 1565
NheI	GCTAGC	1 cut(s)	2281
NlaIV	GGNNCC	3 cut(s)	1015, 1299, 1797
NmuCI	GTSAC	3 cut(s)	59, 289, 2300
NsiI	ATGCAT	2 cut(s)	715, 2347
NspI	RCATGY	3 cut(s)	616, 1787, 2345
PaeI	GCATGC	2 cut(s)	1787, 2345
PaeR7I	CTCGAG	1 cut(s)	2556
PagI	TCATGA	2 cut(s)	685, 2290
PciSI	GCTCTTC	1 cut(s)	2226
PcsI	WCGNNNNNNNCGW	2 cut(s)	403, 1951
PctI	GAATGC	1 cut(s)	1644
PdmI	GAANNNNTTC	2 cut(s)	864, 1941
PfeI	GAWTC	6 cut(s)	190, 394, 431, 568, 2107, 2689
PflFI	GACNNNGTC	1 cut(s)	199
PfoI	TCCNGGA	1 cut(s)	1588
PkrI	GCNGC	8 cut(s)	212, 375, 448, 735, 1111, 1696, 2125, 2238
Ple19I	CGATCG	1 cut(s)	1722
PleI	GAGTC	4 cut(s)	190, 253, 911, 1339
PpsI	GAGTC	4 cut(s)	190, 253, 911, 1339
Ppu21I	YACGTR	1 cut(s)	1074
PpuMI	RGGWCCY	2 cut(s)	665, 1129
PshBI	ATTAAT	1 cut(s)	1704
PsiI	TTATAA	1 cut(s)	2816
Psp124BI	GAGCTC	1 cut(s)	514
Psp1406I	AACGTT	1 cut(s)	406
Psp5II	RGGWCCY	2 cut(s)	665, 1129
Psp6I	CCWGG	6 cut(s)	1016, 1300, 1588, 1948, 2163, 2362
PspGI	CCWGG	6 cut(s)	1016, 1300, 1588, 1948, 2163, 2362
PspN4I	GGNNCC	3 cut(s)	1015, 1299, 1797
PspPI	GGNCC	8 cut(s)	4, 167, 201, 665, 883, 1129, 1583, 2939
PspPPI	RGGWCCY	2 cut(s)	665, 1129
PstNI	CAGNNNCTG	2 cut(s)	193, 2359
PsuI	RGATCY	6 cut(s)	415, 1263, 1492, 1795, 2157, 2875
PsyI	GACNNNGTC	1 cut(s)	199
PvuI	CGATCG	1 cut(s)	1722
PvuII	CAGCTG	1 cut(s)	390
RsaI	GTAC	3 cut(s)	1076, 2217, 2747
RsaNI	GTAC	3 cut(s)	1075, 2216, 2746
RseI	CAYNNNNRTG	1 cut(s)	2135
SacI	GAGCTC	1 cut(s)	514
SacII	CCGCGG	1 cut(s)	214
SalI	GTCGAC	1 cut(s)	1965
SapI	GCTCTTC	1 cut(s)	2226
SaqAI	TTAA	7 cut(s)	329, 603, 779, 1704, 2606, 2871, 3010
SatI	GCNGC	8 cut(s)	211, 374, 447, 734, 1110, 1695, 2124, 2237
Sau96I	GGNCC	8 cut(s)	4, 167, 201, 665, 883, 1129, 1583, 2939
SchI	GAGTC	4 cut(s)	190, 254, 911, 1340
ScrFI	CCNGG	8 cut(s)	121, 1018, 1302, 1565, 1590, 1950, 2165, 2364
SduI	GDGCHC	2 cut(s)	514, 2022
SfaNI	GCATC	5 cut(s)	700, 1329, 1756, 1978, 2439
Sfr274I	CTCGAG	1 cut(s)	2556
Sfr303I	CCGCGG	1 cut(s)	214
SgrBI	CCGCGG	1 cut(s)	214
SinI	GGWCC	7 cut(s)	4, 201, 665, 883, 1129, 1583, 2939
SlaI	CTCGAG	1 cut(s)	2556
SmiMI	CAYNNNNRTG	1 cut(s)	2135
SmlI	CTYRAG	4 cut(s)	419, 1222, 2201, 2556
SmoI	CTYRAG	4 cut(s)	419, 1222, 2201, 2556
SnaBI	TACGTA	1 cut(s)	1074
SphI	GCATGC	2 cut(s)	1787, 2345
SsiI	CCGC	8 cut(s)	211, 213, 223, 355, 734, 1409, 2074, 2965
SspMI	CTAG	3 cut(s)	1059, 1196, 2282
SstI	GAGCTC	1 cut(s)	514
StyD4I	CCNGG	8 cut(s)	119, 1016, 1300, 1563, 1588, 1948, 2163, 2362
StyI	CCWWGG	3 cut(s)	1461, 1626, 2419
TaaI	ACNGT	7 cut(s)	295, 767, 971, 1964, 1971, 2024, 2080
TaiI	ACGT	2 cut(s)	409, 1076
TaqII	GACCGA	1 cut(s)	189
TatI	WGTACW	1 cut(s)	2215
TauI	GCSGC	2 cut(s)	213, 736
TfiI	GAWTC	6 cut(s)	190, 394, 431, 568, 2107, 2689
Tru1I	TTAA	7 cut(s)	329, 603, 779, 1704, 2606, 2871, 3010
Tru9I	TTAA	7 cut(s)	329, 603, 779, 1704, 2606, 2871, 3010
TscAI	CASTG	8 cut(s)	255, 570, 772, 2029, 2083, 2142, 2982, 3003
TseFI	GTSAC	3 cut(s)	59, 289, 2300
TseI	GCWGC	6 cut(s)	373, 446, 1109, 1694, 2123, 2236
Tsp45I	GTSAC	3 cut(s)	59, 289, 2300
TspGWI	ACGGA	1 cut(s)	2931
TspRI	CASTG	8 cut(s)	255, 570, 772, 2029, 2083, 2142, 2982, 3003
Tth111I	GACNNNGTC	1 cut(s)	199
VneI	GTGCAC	1 cut(s)	2018
VpaK11BI	GGWCC	7 cut(s)	4, 201, 665, 883, 1129, 1583, 2939
VspI	ATTAAT	1 cut(s)	1704
XagI	CCTNNNNNAGG	1 cut(s)	890
XapI	RAATTY	8 cut(s)	114, 157, 819, 944, 1414, 1937, 2007, 2581
XceI	RCATGY	3 cut(s)	616, 1787, 2345
XhoI	CTCGAG	1 cut(s)	2556
XmiI	GTMKAC	3 cut(s)	1966, 2052, 2415
XmnI	GAANNNNTTC	2 cut(s)	864, 1941
XspI	CTAG	3 cut(s)	1059, 1196, 2282
Zsp2I	ATGCAT	2 cut(s)	715, 2347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.