Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0010434

Physical Location & Seq

Genomic Coordinates Reverse (-)

19156588 .. 19166022

Gene Structure 9435 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG173500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2805 bp

ATGGACCTCATACTCCCCTACAAGCCAAACCCACTTCTCTGTTCTTCAACTCCACTCACCCATTTAACCCCCAAAACCCTTCTCTTCAAGCTCCCCACAAAGCAAAGACTCAAAATTTCCCGGAAAAATTCAAACTTTCGTGTTAAAGCCTCTGCAAACCCAAATGGGCCTGATGGGTTTTCTTGGGTGAGTCTGGCTCGGTCTATACGCCGCGGTTCGGAGCAGTTCTGGTCCAATTTTGGTGACTCGGTGAAGAAGGAAACTGGGTTTGATTTGAATGAGGTGAATGTGAAGGTGGGGGAGTATTTGGGGCAAGCTGGGGAAGGATTAAAGAGAGGTGGGACTGAGTTGGAACGGTTTAGGACCGAGTTCGTGCCCGAGTTTGTGAGTTGGAATCAGTGGGAAAGATGGAAGGATGTCAAGACATGGGAACCTAAAAGAATTGCTGCATTGGTTTTCTATGTTTTTGTTGCGGTAGTTTCGTGCCAAAGAATATATGTAGCAATTCAAGCTCCTATCCAAGATCGTCAGAGAAAAGAGTTGACAGAGGCTTATATGGAGGCAGTGATTCCTGAGCCATCTCCGAGTAATGTTAGAAAGCTTAAGAAGAGTATGTGGAGGAAGATGACGCCCAAAGGCCTGAAAATGAAGAAGTTTGTTGAAGGACCTGATGGAACACTTGTCCATGATAGTTCTTATGTGGGAGAGGATGCATGGGATGATGATCCGCAGCTGCCTCAGGACAATGTGAAACAATTTATTGACAGTGATATAAAATTAAATCCAGCAGAAAAGAAGGAATTGAAAGAAGACCTGGGAATATCTGGTGAAGTTCAAGAGGATACTGGAACATGGCGTGAAAGACTTCAGAAATGGAAGGAGATCCTCCAAAAAGAAAAGTTAGCGGAGCAGTTGGACTCTGCAAATTCCAAGTATGTAGTTGAATTTGACATGAAAGAGGTTGAAAACAGTCTTCGCAAGGATGTAGTGGAGAAGGTAACAGAGACACAAGGAACAAGGGCGTTATGGATAGCTAAAAGATGGTGGCTTTATCGTCCCAAACTTCCTTATACCTACTTTCTTCAAAAGCTTGACAGCTCTGAGGTTGCTGCTGTTGTATTTACAGAAGACCTAAAAAGAATATATGTTACTATGAAAGAAGGTTTTCCATTAGAATATGTTGTTGATATCCCCCTAGACCCGTATTTGTTTGAGAATATCTCAAGTTCTGGAGCTGAAGTTGATCTTCTTCAGAAACGCCAGATCCACTACTTTATGAAGGTTGTGATTGCATTGGTGCCTGGGTTACTCATTCTCTGGCTAATACGCGAGTCTGTGATGCTTCTGCATATCACTTCTAAGCGTTTTCTCTACAAAAAGTATAATCAACTTTTTGATATGGCTTATGCAGAAAATTTTATCCTGCCTGTTGGAGATGTAGGGGAAACAAAATCCATGTCCAAGGAAGTTGTGTTAGGAGGTGATGTTTGGGATCTTCTTGATGAGCTGATGATTTACATGGGAAATCCAATGCAATACTATGAAAAAGATGTGAAGTTTGTCAGGGGTGTTCTTCTCTCTGGACCTCCTGGAACTGGCAAAACACTTTTTGCAAGGACACTTGCAAAGGAAAGTGGGTTGCCATTTGTTTTTGCTTCGGGTGCGGAGTTTACAGATAGTGAAAAGAGTGGTGCTGCAAAAATCAATGAGATGTTTTCTATTGCAAGGAGAAATGCTCCTTGTTTTGTATTTGTGGATGAGATTGATGCTATTGCTGGAAGGCATGCAAGACAGGATCCTAGAAGAAGAGCAACATTTGAGGCTTTGATTGCACAGCTTGATGGGGAGAAGGAAAAAACTGGCATTGATCGCTTCTCTCTCAGACAAGCTGTGATATTCATCTGTGCTACCAACAGGCCAGATGAATTAGACCTTGAATTTGTTCGATCTGGACGTATTGACCGTCGACTGTACATTGGTTTGCCTGATGCAAAGCAACAAGTGCAAATTTTTAAAGTGCACAGCGCAGGAAAGCAACTTGCAGGAGATGTAGACTTTGGAAAAGTTGTCTTCCGTACGGTTGGTTTTTCTGGGGCAGATATTCGGAATCTTGTAAATGAAGCAGCAATTATGTCAGTGAGGAAAGGACGTTCAGAGATATATCAGGAAGACATAGTGGATGTATTAGATAAACAATTGCTTGAGGGCATGGGTGTACTTCTTACTGAGGAAGAGCAGCGGAAATGCGAACAAAGTGTGTCTTCTGAAAAGAAGAAACTGCTGGCTGTTCATGAAGCTGGTCACATACTATTAGCTCATCTGTTTCCTCAATTTGACTGGCATGCATTTTCTCAACTTCTGCCTGGTGGGAAGGAAACTGCAGTATCTGTATTCTATCCCCGAGAAGATATGGTAGACCAAGGTTATACAACCTTTGGCTACATGAAGATGCAGATGGTGGTAGCTCATGGTGGGCGTTGCGCTGAACGTGTTGTGTATGGTGATGACATAACTGATGGAGGAAGAGATGATCTGGAAAAGCTAACAAAGATTGCTCGAGAGATGGTAATTAGCCCTCAAAACTCAAGATTGGGGCTTACAGCTCTAACAAAAAGAATTGGACTAATGGATCGACCAGATAGTCCAGATGGCGAATTGATAAGATACAGGTGGGAGGACCCCAATGTGATTCCAGCCAATATGACTCTTGAAGTGTCTGAGCTATTTACCCGAGAGTTGACAAGGGTGCGTAATTTGATTTTTGTTTATTATCAATCGCCTGCTATACTAAAAGGGTACTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

934

Amino Acids

106.75

Weight (kDa)

7.17

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	524 - 660	4.9e-34	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	683 - 725	5.1e-13	AAA+ lid domain
Peptidase_M41	PF01434	753 - 858	2.4e-18	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79560 AT1G79560 AT1G79560
fragaria_vesca	FvH4_3g14760 FvH4_3g14760 FvH4_3g14760 FvH4_3g14760
malus_domestica	MD05G1229800.v1.1
prunus_persica	Prupe.4G132700_v2.0.a1
pyrus_communis	pycom05g21070
rosa_chinensis	RchiOBHm_Chr5g0024281
rosa_laevigata	RLG00000032790
rosa_multiflora	Rmu_sc0002344.1_g000002
rosa_roxburghii	Rroxscaffold_1G00054900 Rroxscaffold_1G00055780
rosa_rugosa	Rorug05G0083000
rosa_samantha	Rh5AG173500 Rh5CG186500 Rh5DG173000
rosa_wichuraiana	Rw0G000870 Rw5G015670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2640
AccB1I	GGYRCC	1 cut(s)	1297
AccI	GTMKAC	3 cut(s)	1966, 2052, 2415
AccII	CGCG	2 cut(s)	213, 1329
AciI	CCGC	7 cut(s)	211, 213, 473, 728, 905, 1664, 2239
AclWI	GGATC	7 cut(s)	719, 877, 1258, 1500, 1790, 1803, 2637
AcsI	RAATTY	7 cut(s)	114, 127, 925, 944, 1414, 1937, 2007
AcuI	CTGAAG	3 cut(s)	851, 1235, 1257
AcyI	GRCGYC	1 cut(s)	629
AfaI	GTAC	4 cut(s)	1973, 2077, 2217, 2798
AfiI	CCNNNNNNNGG	2 cut(s)	217, 1595
AflII	CTTAAG	1 cut(s)	602
AflIII	ACRYGT	1 cut(s)	2488
AjnI	CCWGG	4 cut(s)	813, 1300, 1588, 2362
AloI	GAACNNNNNNTCC	2 cut(s)	2134, 2166
Alw21I	GWGCWC	1 cut(s)	2022
Alw26I	GTCTC	1 cut(s)	998
Alw44I	GTGCAC	1 cut(s)	2018
AlwI	GGATC	7 cut(s)	719, 877, 1258, 1500, 1790, 1803, 2637
AlwNI	CAGNNNCTG	1 cut(s)	2387
Ama87I	CYCGRG	4 cut(s)	377, 2400, 2556, 2730
AoxI	GGCC	3 cut(s)	167, 637, 1916
ApaLI	GTGCAC	1 cut(s)	2018
ApeKI	GCWGC	7 cut(s)	446, 730, 733, 1109, 1694, 2123, 2236
ApoI	RAATTY	7 cut(s)	114, 127, 925, 944, 1414, 1937, 2007
ArsI	GACNNNNNNTTYG	4 cut(s)	1442, 1474, 1922, 1954
Asp700I	GAANNNNTTC	3 cut(s)	864, 1164, 1941
AspLEI	GCGC	2 cut(s)	2027, 2483
AspS9I	GGNCC	7 cut(s)	4, 167, 231, 363, 665, 1583, 2677
AsuC2I	CCSGG	1 cut(s)	121
AsuHPI	GGTGA	8 cut(s)	49, 199, 254, 262, 295, 839, 1493, 2513
AvaI	CYCGRG	4 cut(s)	377, 2400, 2556, 2730
AvaII	GGWCC	6 cut(s)	4, 231, 363, 665, 1583, 2677
AxyI	CCTNAGG	1 cut(s)	738
BaeGI	GKGCMC	2 cut(s)	378, 2022
BaeI	ACNNNNGTAYC	2 cut(s)	2367, 2400
BamHI	GGATCC	1 cut(s)	1795
BanI	GGYRCC	1 cut(s)	1297
BbsI	GAAGAC	6 cut(s)	816, 965, 1134, 2062, 2175, 2253
Bbv12I	GWGCWC	1 cut(s)	2022
BbvI	GCAGC	7 cut(s)	433, 720, 742, 1096, 1681, 2135, 2248
BciT130I	CCWGG	4 cut(s)	815, 1302, 1590, 2364
BciVI	GTATCC	1 cut(s)	835
BcnI	CCSGG	1 cut(s)	121
BcoDI	GTCTC	1 cut(s)	998
BfaI	CTAG	2 cut(s)	1196, 1800
BfmI	CTRYAG	1 cut(s)	2379
BfrI	CTTAAG	1 cut(s)	602
BfuI	GTATCC	1 cut(s)	835
BisI	GCNGC	8 cut(s)	211, 447, 731, 734, 1110, 1695, 2124, 2237
BlsI	GCNGC	8 cut(s)	212, 448, 732, 735, 1111, 1696, 2125, 2238
Bme1390I	CCNGG	5 cut(s)	121, 815, 1302, 1590, 2364
Bme18I	GGWCC	6 cut(s)	4, 231, 363, 665, 1583, 2677
BmeT110I	CYCGRG	4 cut(s)	377, 2400, 2556, 2730
BmgT120I	GGNCC	7 cut(s)	4, 167, 231, 363, 665, 1583, 2677
BmiI	GGNNCC	4 cut(s)	432, 1299, 1797, 2679
BmrFI	CCNGG	5 cut(s)	121, 815, 1302, 1590, 2364
BmrI	ACTGGG	1 cut(s)	273
BmsI	GCATC	5 cut(s)	700, 1329, 1756, 1978, 2439
BmuI	ACTGGG	1 cut(s)	273
BpiI	GAAGAC	6 cut(s)	816, 965, 1134, 2062, 2175, 2253
BplI	GAGNNNNNCTC	6 cut(s)	181, 213, 1205, 1237, 1720, 1752
BpmI	CTGGAG	1 cut(s)	1251
Bpu10I	CCTNAGC	1 cut(s)	573
BpuEI	CTTGAG	3 cut(s)	1207, 2222, 2569
BpuMI	CCSGG	1 cut(s)	121
BsaBI	GATNNNNATC	2 cut(s)	723, 1898
BsaHI	GRCGYC	1 cut(s)	629
BsaJI	CCNNGG	5 cut(s)	211, 814, 1301, 1461, 2419
BsaXI	ACNNNNNCTCC	4 cut(s)	1224, 1254, 1470, 1500
Bsc4I	CCNNNNNNNGG	2 cut(s)	217, 1595
Bse1I	ACTGG	5 cut(s)	268, 850, 1600, 1864, 2342
Bse21I	CCTNAGG	1 cut(s)	738
Bse8I	GATNNNNATC	2 cut(s)	723, 1898
BseBI	CCWGG	4 cut(s)	815, 1302, 1590, 2364
BseDI	CCNNGG	5 cut(s)	211, 814, 1301, 1461, 2419
BseGI	GGATG	6 cut(s)	421, 715, 724, 988, 1762, 2185
BseJI	GATNNNNATC	2 cut(s)	723, 1898
BseLI	CCNNNNNNNGG	2 cut(s)	217, 1595
BseMII	CTCAG	7 cut(s)	336, 564, 752, 1092, 1894, 2217, 2709
BseNI	ACTGG	5 cut(s)	268, 850, 1600, 1864, 2342
BseSI	GKGCMC	2 cut(s)	378, 2022
BseXI	GCAGC	7 cut(s)	433, 720, 742, 1096, 1681, 2135, 2248
BseYI	CCCAGC	1 cut(s)	317
Bsh1236I	CGCG	2 cut(s)	213, 1329
BshFI	GGCC	3 cut(s)	169, 639, 1918
BshNI	GGYRCC	1 cut(s)	1297
BsiHKAI	GWGCWC	1 cut(s)	2022
BsiHKCI	CYCGRG	4 cut(s)	377, 2400, 2556, 2730
BsiSI	CCGG	1 cut(s)	121
BsiWI	CGTACG	1 cut(s)	2075
BslFI	GGGAC	2 cut(s)	355, 1041
BslI	CCNNNNNNNGG	2 cut(s)	217, 1595
BsmAI	GTCTC	1 cut(s)	998
BsmFI	GGGAC	2 cut(s)	355, 1041
BsnI	GGCC	3 cut(s)	169, 639, 1918
BsoBI	CYCGRG	4 cut(s)	377, 2400, 2556, 2730
Bsp1286I	GDGCHC	2 cut(s)	378, 2022
Bsp1407I	TGTACA	1 cut(s)	1971
BspACI	CCGC	7 cut(s)	211, 213, 473, 728, 905, 1664, 2239
BspANI	GGCC	3 cut(s)	169, 639, 1918
BspCNI	CTCAG	7 cut(s)	337, 565, 751, 1093, 1893, 2218, 2710
BspFNI	CGCG	2 cut(s)	213, 1329
BspHI	TCATGA	1 cut(s)	2290
BspLI	GGNNCC	4 cut(s)	432, 1299, 1797, 2679
BspMAI	CTGCAG	1 cut(s)	2383
BspPI	GGATC	7 cut(s)	719, 877, 1258, 1500, 1790, 1803, 2637
BspQI	GCTCTTC	2 cut(s)	1801, 2226
BspT107I	GGYRCC	1 cut(s)	1297
BspTI	CTTAAG	1 cut(s)	602
BsrGI	TGTACA	1 cut(s)	1971
BsrI	ACTGG	5 cut(s)	268, 850, 1600, 1864, 2342
BssECI	CCNNGG	5 cut(s)	211, 814, 1301, 1461, 2419
BssNI	GRCGYC	1 cut(s)	629
BssT1I	CCWWGG	2 cut(s)	1461, 2419
Bst2UI	CCWGG	4 cut(s)	815, 1302, 1590, 2364
Bst4CI	ACNGT	6 cut(s)	357, 767, 971, 1964, 1971, 2080
Bst6I	CTCTTC	5 cut(s)	89, 602, 1801, 2226, 2518
BstACI	GRCGYC	1 cut(s)	629
BstAFI	CTTAAG	1 cut(s)	602
BstAPI	GCANNNNNTGC	1 cut(s)	2002
BstAUI	TGTACA	1 cut(s)	1971
BstC8I	GCNNGC	5 cut(s)	315, 1785, 2283, 2343, 2781
BstDEI	CTNAG	8 cut(s)	345, 573, 738, 1101, 1359, 1880, 2226, 2718
BstDSI	CCRYGG	1 cut(s)	211
BstF5I	GGATG	6 cut(s)	421, 715, 724, 988, 1762, 2185
BstFNI	CGCG	2 cut(s)	213, 1329
BstHHI	GCGC	2 cut(s)	2027, 2483
BstMAI	GTCTC	1 cut(s)	998
BstMWI	GCNNNNNNNGC	5 cut(s)	509, 1661, 1829, 1869, 2002
BstNI	CCWGG	4 cut(s)	815, 1302, 1590, 2364
BstNSI	RCATGY	2 cut(s)	1787, 2345
BstSCI	CCNGG	5 cut(s)	119, 813, 1300, 1588, 2362
BstSFI	CTRYAG	1 cut(s)	2379
BstSLI	GKGCMC	2 cut(s)	378, 2022
BstUI	CGCG	2 cut(s)	213, 1329
BstV1I	GCAGC	7 cut(s)	433, 720, 742, 1096, 1681, 2135, 2248
BstV2I	GAAGAC	6 cut(s)	816, 965, 1134, 2062, 2175, 2253
BstX2I	RGATCY	4 cut(s)	882, 1263, 1492, 1795
BstYI	RGATCY	4 cut(s)	882, 1263, 1492, 1795
Bsu36I	CCTNAGG	1 cut(s)	738
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	3 cut(s)	169, 639, 1918
BtgI	CCRYGG	1 cut(s)	211
BtsCI	GGATG	6 cut(s)	421, 715, 724, 988, 1762, 2185
BtsI	GCAGTG	1 cut(s)	570
BtsIMutI	CAGTG	4 cut(s)	404, 570, 772, 2142
Cac8I	GCNNGC	5 cut(s)	315, 1785, 2283, 2343, 2781
CaiI	CAGNNNCTG	1 cut(s)	2387
CciI	TCATGA	1 cut(s)	2290
CfoI	GCGC	2 cut(s)	2027, 2483
Cfr13I	GGNCC	7 cut(s)	4, 167, 231, 363, 665, 1583, 2677
Cfr42I	CCGCGG	1 cut(s)	214
CseI	GACGC	1 cut(s)	637
Csp6I	GTAC	4 cut(s)	1972, 2076, 2216, 2797
CviQI	GTAC	4 cut(s)	1972, 2076, 2216, 2797
DdeI	CTNAG	8 cut(s)	345, 573, 738, 1101, 1359, 1880, 2226, 2718
DraI	TTTAAA	1 cut(s)	2014
DrdI	GACNNNNNNGTC	1 cut(s)	2640
DseDI	GACNNNNNNGTC	1 cut(s)	2640
Eam1104I	CTCTTC	5 cut(s)	89, 602, 1801, 2226, 2518
EarI	CTCTTC	5 cut(s)	89, 602, 1801, 2226, 2518
Eco130I	CCWWGG	2 cut(s)	1461, 2419
Eco147I	AGGCCT	1 cut(s)	639
Eco32I	GATATC	1 cut(s)	1189
Eco47I	GGWCC	6 cut(s)	4, 231, 363, 665, 1583, 2677
Eco57I	CTGAAG	3 cut(s)	851, 1235, 1257
Eco81I	CCTNAGG	1 cut(s)	738
Eco88I	CYCGRG	4 cut(s)	377, 2400, 2556, 2730
EcoO109I	RGGNCCY	2 cut(s)	665, 2677
EcoRII	CCWGG	4 cut(s)	813, 1300, 1588, 2362
EcoRV	GATATC	1 cut(s)	1189
EcoT14I	CCWWGG	2 cut(s)	1461, 2419
EcoT22I	ATGCAT	2 cut(s)	715, 2347
ErhI	CCWWGG	2 cut(s)	1461, 2419
FalI	AAGNNNNNCTT	4 cut(s)	1230, 1262, 2245, 2277
FaqI	GGGAC	2 cut(s)	355, 1041
FblI	GTMKAC	3 cut(s)	1966, 2052, 2415
Fnu4HI	GCNGC	8 cut(s)	211, 447, 731, 734, 1110, 1695, 2124, 2237
FokI	GGATG	6 cut(s)	428, 722, 731, 995, 1769, 2192
Fsp4HI	GCNGC	8 cut(s)	211, 447, 731, 734, 1110, 1695, 2124, 2237
FspBI	CTAG	2 cut(s)	1196, 1800
GlaI	GCGC	2 cut(s)	2026, 2482
GluI	GCNGC	8 cut(s)	211, 447, 731, 734, 1110, 1695, 2124, 2237
GsaI	CCCAGC	1 cut(s)	321
GsuI	CTGGAG	1 cut(s)	1251
HaeIII	GGCC	3 cut(s)	169, 639, 1918
HapII	CCGG	1 cut(s)	121
HgaI	GACGC	1 cut(s)	637
HhaI	GCGC	2 cut(s)	2027, 2483
Hin1I	GRCGYC	1 cut(s)	629
Hin6I	GCGC	2 cut(s)	2025, 2481
HinP1I	GCGC	2 cut(s)	2025, 2481
HincII	GTYRAC	3 cut(s)	543, 1967, 2739
HindII	GTYRAC	3 cut(s)	543, 1967, 2739
HindIII	AAGCTT	2 cut(s)	599, 1088
HpaII	CCGG	1 cut(s)	121
HphI	GGTGA	8 cut(s)	49, 199, 254, 262, 295, 839, 1493, 2513
Hpy166II	GTNNAC	8 cut(s)	543, 1671, 1967, 2020, 2053, 2216, 2416, 2739
Hpy8I	GTNNAC	8 cut(s)	543, 1671, 1967, 2020, 2053, 2216, 2416, 2739
Hpy99I	CGWCG	1 cut(s)	1968
HpyCH4III	ACNGT	6 cut(s)	357, 767, 971, 1964, 1971, 2080
HpyCH4IV	ACGT	3 cut(s)	1954, 2149, 2488
HpyF10VI	GCNNNNNNNGC	5 cut(s)	509, 1661, 1829, 1869, 2002
HpyF3I	CTNAG	8 cut(s)	345, 573, 738, 1101, 1359, 1880, 2226, 2718
HpySE526I	ACGT	3 cut(s)	1954, 2149, 2488
Hsp92I	GRCGYC	1 cut(s)	629
HspAI	GCGC	2 cut(s)	2025, 2481
KspI	CCGCGG	1 cut(s)	214
LguI	GCTCTTC	2 cut(s)	1801, 2226
LmnI	GCTCC	6 cut(s)	96, 220, 517, 907, 1232, 1741
Lsp1109I	GCAGC	7 cut(s)	433, 720, 742, 1096, 1681, 2135, 2248
LweI	GCATC	5 cut(s)	700, 1329, 1756, 1978, 2439
MaeI	CTAG	2 cut(s)	1196, 1800
MaeII	ACGT	3 cut(s)	1954, 2149, 2488
MaeIII	GTNAC	5 cut(s)	242, 997, 1147, 1305, 2300
MfeI	CAATTG	1 cut(s)	2195
MflI	RGATCY	4 cut(s)	882, 1263, 1492, 1795
MhlI	GDGCHC	2 cut(s)	378, 2022
MlyI	GAGTC	6 cut(s)	102, 199, 239, 911, 1340, 2698
MmeI	TCCRAC	4 cut(s)	330, 371, 894, 1411
Mph1103I	ATGCAT	2 cut(s)	715, 2347
MroXI	GAANNNNTTC	3 cut(s)	864, 1164, 1941
MseI	TTAA	6 cut(s)	65, 144, 329, 603, 779, 2013
MslI	CAYNNNNRTG	1 cut(s)	2447
MspA1I	CMGCKG	3 cut(s)	213, 733, 2239
MspCI	CTTAAG	1 cut(s)	602
MspI	CCGG	1 cut(s)	121
MspR9I	CCNGG	5 cut(s)	121, 815, 1302, 1590, 2364
MunI	CAATTG	1 cut(s)	2195
MvaI	CCWGG	4 cut(s)	815, 1302, 1590, 2364
MvnI	CGCG	2 cut(s)	213, 1329
MwoI	GCNNNNNNNGC	5 cut(s)	509, 1661, 1829, 1869, 2002
NciI	CCSGG	1 cut(s)	121
NlaIV	GGNNCC	4 cut(s)	432, 1299, 1797, 2679
NmuCI	GTSAC	2 cut(s)	242, 2300
NsiI	ATGCAT	2 cut(s)	715, 2347
NspI	RCATGY	2 cut(s)	1787, 2345
PaeI	GCATGC	2 cut(s)	1787, 2345
PaeR7I	CTCGAG	1 cut(s)	2556
PagI	TCATGA	1 cut(s)	2290
PceI	AGGCCT	1 cut(s)	639
PciSI	GCTCTTC	2 cut(s)	1801, 2226
PcsI	WCGNNNNNNNCGW	2 cut(s)	1951, 1960
PdmI	GAANNNNTTC	3 cut(s)	864, 1164, 1941
PfeI	GAWTC	4 cut(s)	394, 568, 2107, 2689
Pfl23II	CGTACG	1 cut(s)	2075
PfoI	TCCNGGA	2 cut(s)	119, 1588
PkrI	GCNGC	8 cut(s)	212, 448, 732, 735, 1111, 1696, 2125, 2238
PleI	GAGTC	6 cut(s)	102, 198, 239, 911, 1339, 2698
PpsI	GAGTC	6 cut(s)	102, 198, 239, 911, 1339, 2698
PpuMI	RGGWCCY	2 cut(s)	665, 2677
Psp5II	RGGWCCY	2 cut(s)	665, 2677
Psp6I	CCWGG	4 cut(s)	813, 1300, 1588, 2362
PspFI	CCCAGC	1 cut(s)	317
PspGI	CCWGG	4 cut(s)	813, 1300, 1588, 2362
PspLI	CGTACG	1 cut(s)	2075
PspN4I	GGNNCC	4 cut(s)	432, 1299, 1797, 2679
PspPI	GGNCC	7 cut(s)	4, 167, 231, 363, 665, 1583, 2677
PspPPI	RGGWCCY	2 cut(s)	665, 2677
PstI	CTGCAG	1 cut(s)	2383
PstNI	CAGNNNCTG	1 cut(s)	2387
PsuI	RGATCY	4 cut(s)	882, 1263, 1492, 1795
PvuII	CAGCTG	1 cut(s)	733
RsaI	GTAC	4 cut(s)	1973, 2077, 2217, 2798
RsaNI	GTAC	4 cut(s)	1972, 2076, 2216, 2797
RseI	CAYNNNNRTG	1 cut(s)	2447
SacII	CCGCGG	1 cut(s)	214
SalI	GTCGAC	1 cut(s)	1965
SapI	GCTCTTC	2 cut(s)	1801, 2226
SaqAI	TTAA	6 cut(s)	65, 144, 329, 603, 779, 2013
SatI	GCNGC	8 cut(s)	211, 447, 731, 734, 1110, 1695, 2124, 2237
Sau96I	GGNCC	7 cut(s)	4, 167, 231, 363, 665, 1583, 2677
SchI	GAGTC	6 cut(s)	102, 199, 239, 911, 1340, 2698
ScrFI	CCNGG	5 cut(s)	121, 815, 1302, 1590, 2364
SduI	GDGCHC	2 cut(s)	378, 2022
SfaNI	GCATC	5 cut(s)	700, 1329, 1756, 1978, 2439
SfcI	CTRYAG	1 cut(s)	2379
Sfr274I	CTCGAG	1 cut(s)	2556
Sfr303I	CCGCGG	1 cut(s)	214
SgrBI	CCGCGG	1 cut(s)	214
SinI	GGWCC	6 cut(s)	4, 231, 363, 665, 1583, 2677
SlaI	CTCGAG	1 cut(s)	2556
SmiMI	CAYNNNNRTG	1 cut(s)	2447
SmlI	CTYRAG	5 cut(s)	602, 1222, 2201, 2556, 2584
SmoI	CTYRAG	5 cut(s)	602, 1222, 2201, 2556, 2584
SphI	GCATGC	2 cut(s)	1787, 2345
SseBI	AGGCCT	1 cut(s)	639
SsiI	CCGC	7 cut(s)	211, 213, 473, 728, 905, 1664, 2239
SspMI	CTAG	2 cut(s)	1196, 1800
StuI	AGGCCT	1 cut(s)	639
StyD4I	CCNGG	5 cut(s)	119, 813, 1300, 1588, 2362
StyI	CCWWGG	2 cut(s)	1461, 2419
TaaI	ACNGT	6 cut(s)	357, 767, 971, 1964, 1971, 2080
TaiI	ACGT	3 cut(s)	1957, 2152, 2491
TaqI	TCGA	4 cut(s)	1945, 1966, 2557, 2632
TaqII	GACCGA	2 cut(s)	189, 380
TatI	WGTACW	2 cut(s)	1971, 2215
TauI	GCSGC	1 cut(s)	213
TfiI	GAWTC	4 cut(s)	394, 568, 2107, 2689
Tru1I	TTAA	6 cut(s)	65, 144, 329, 603, 779, 2013
Tru9I	TTAA	6 cut(s)	65, 144, 329, 603, 779, 2013
TscAI	CASTG	4 cut(s)	404, 570, 772, 2142
TseFI	GTSAC	2 cut(s)	242, 2300
TseI	GCWGC	7 cut(s)	446, 730, 733, 1109, 1694, 2123, 2236
Tsp45I	GTSAC	2 cut(s)	242, 2300
TspGWI	ACGGA	1 cut(s)	2063
TspRI	CASTG	4 cut(s)	404, 570, 772, 2142
Vha464I	CTTAAG	1 cut(s)	602
VneI	GTGCAC	1 cut(s)	2018
VpaK11BI	GGWCC	6 cut(s)	4, 231, 363, 665, 1583, 2677
XapI	RAATTY	7 cut(s)	114, 127, 925, 944, 1414, 1937, 2007
XceI	RCATGY	2 cut(s)	1787, 2345
XhoI	CTCGAG	1 cut(s)	2556
XmiI	GTMKAC	3 cut(s)	1966, 2052, 2415
XmnI	GAANNNNTTC	3 cut(s)	864, 1164, 1941
XspI	CTAG	2 cut(s)	1196, 1800
Zsp2I	ATGCAT	2 cut(s)	715, 2347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.