MD05G1240300.v1.1

Class I heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016287
Physical Location & Seq
Reverse (-)
37103604 .. 37104083
480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1240300.v1.1.491

Sequence Viewer

Length: 480 bp
ATGTCCCTTTTATTACAATCTCTCTTGGACCAACCCAACTTTGCTCCTTTCAAAGTTTTCGACCCAGAAATATCCAACCAAAACAACACGTACATGGACTGGAAGGAGACTTCAAATGCACACATCTTCGAGATAGATCTTCCAGGCCTTACAAAAGAGGACGTGAAGCTGGAGCTGCAAGAAAACCAAGTGCTTCATGTTAGTGCAGAGAGAAAAGCGGAGCCCGAAATGGAGGACCCCAAGAACGAGACGTGGCACTGCAGGGAGAGGGAAAGTGGCAACTTCTCGAGGAATTTCAGGTTGCCTGAAAATGTCAAGGTTGATGAGATTAAGGCTTCAATGCGCGACGGGGTGTTGGTAATTACAGTGCCTAAGGAGGACTTGAAGAAGAAACACAAGCATAAGAAGGTTGAGATCTCTGGGGATGATGAAGGACATGCTTCCAAAGGACTTGGACGTTTCGTGTGCTGCAGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

160

Amino Acids

18.32

Weight (kDa)

5.72

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 32 - 125 9.2e-06 CS domain
HSP20 PF00011 33 - 140 5.7e-27 Hsp20/alpha crystallin family
ArsA_HSP20 PF17886 43 - 122 2.5e-06 HSP20-like domain found in ArsA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016287)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13900
malus_domestica MD05G1240300.v1.1 MD10G1218300.v1.1
prunus_persica Prupe.4G125800_v2.0.a1
pyrus_communis pycom05g21630
rosa_chinensis RchiOBHm_Chr5g0023111
rosa_laevigata RLG00000032699
rosa_roxburghii Rroxscaffold_1G00056240
rosa_rugosa Rorug05G0077200
rosa_samantha Rh5AG163300 Rh5BG162600 Rh5CG177500 Rh5DG163900
rosa_wichuraiana Rw5G014730 Rw5G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 345
AciI CCGC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 292
AfaI GTAC 1 cut(s) 92
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 4 cut(s) 52, 114, 339, 385
AjiI CACGTC 2 cut(s) 163, 252
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 3 cut(s) 169, 175, 476
AluI AGCT 3 cut(s) 169, 175, 476
Alw26I GTCTC 2 cut(s) 101, 242
Ama87I CYCGRG 1 cut(s) 286
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 2 cut(s) 175, 468
ApoI RAATTY 1 cut(s) 292
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 2 cut(s) 28, 235
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 2 cut(s) 28, 235
AxyI CCTNAGG 1 cut(s) 372
BanII GRGCYC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 162, 455
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 101, 242
BfmI CTRYAG 2 cut(s) 259, 469
BglII AGATCT 2 cut(s) 136, 414
BisI GCNGC 2 cut(s) 176, 469
BlsI GCNGC 2 cut(s) 177, 470
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 28, 235
BmeT110I CYCGRG 1 cut(s) 286
BmgBI CACGTC 2 cut(s) 163, 252
BmgT120I GGNCC 2 cut(s) 28, 235
BmiI GGNNCC 2 cut(s) 222, 237
BmrFI CCNGG 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 90
Bse1I ACTGG 1 cut(s) 104
Bse21I CCTNAGG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 430
BseNI ACTGG 1 cut(s) 104
BseXI GCAGC 2 cut(s) 162, 455
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 286
BsmAI GTCTC 2 cut(s) 101, 242
BsmBI CGTCTC 1 cut(s) 242
BsnI GGCC 1 cut(s) 147
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 2 cut(s) 136, 414
BspACI CCGC 1 cut(s) 218
BspANI GGCC 1 cut(s) 147
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 222, 237
BspMAI CTGCAG 2 cut(s) 263, 473
BsrI ACTGG 1 cut(s) 104
BssMI GATC 2 cut(s) 136, 414
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 2 cut(s) 139, 417
BstMAI GTCTC 2 cut(s) 101, 242
BstMBI GATC 2 cut(s) 136, 414
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 2 cut(s) 259, 469
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 162, 455
BstX2I RGATCY 2 cut(s) 136, 414
BstYI RGATCY 2 cut(s) 136, 414
Bsu36I CCTNAGG 1 cut(s) 372
BsuRI GGCC 1 cut(s) 147
BtrI CACGTC 2 cut(s) 163, 252
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 256, 372
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 28, 235
Csp6I GTAC 1 cut(s) 91
CviAII CATG 3 cut(s) 94, 197, 437
CviJI RGCY 6 cut(s) 147, 169, 175, 223, 335, 476
CviKI_1 RGCY 6 cut(s) 147, 169, 175, 223, 335, 476
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 372
DpnI GATC 2 cut(s) 138, 416
DpnII GATC 2 cut(s) 136, 414
Eco147I AGGCCT 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 28, 235
Eco81I CCTNAGG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 3 cut(s) 97, 200, 440
FaiI YATR 4 cut(s) 95, 198, 402, 438
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 3 cut(s) 93, 196, 436
Fnu4HI GCNGC 2 cut(s) 176, 469
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 2 cut(s) 176, 469
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 176, 469
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 1 cut(s) 147
HhaI GCGC 1 cut(s) 345
Hin1II CATG 3 cut(s) 97, 200, 440
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
Hpy188III TCNNGA 2 cut(s) 130, 286
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 3 cut(s) 97, 400, 425
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 4 cut(s) 89, 162, 251, 457
HpyCH4V TGCA 5 cut(s) 119, 178, 206, 261, 471
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 4 cut(s) 89, 162, 251, 457
Hsp92II CATG 3 cut(s) 97, 200, 440
HspAI GCGC 1 cut(s) 343
Kzo9I GATC 2 cut(s) 136, 414
LmnI GCTCC 3 cut(s) 49, 172, 220
LpnPI CCDG 9 cut(s) 78, 85, 129, 155, 156, 247, 283, 318, 405
Lsp1109I GCAGC 2 cut(s) 162, 455
MaeII ACGT 4 cut(s) 89, 162, 251, 457
MalI GATC 2 cut(s) 138, 416
MboI GATC 2 cut(s) 136, 414
MboII GAAGA 4 cut(s) 118, 131, 397, 400
MflI RGATCY 2 cut(s) 136, 414
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 2 cut(s) 292, 360
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 5 cut(s) 151, 226, 261, 282, 370
MseI TTAA 2 cut(s) 330, 478
MslI CAYNNNNRTG 2 cut(s) 92, 201
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 175
NdeII GATC 2 cut(s) 136, 414
NlaIII CATG 3 cut(s) 97, 200, 440
NlaIV GGNNCC 2 cut(s) 222, 237
NspI RCATGY 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 286
PceI AGGCCT 1 cut(s) 147
PkrI GCNGC 2 cut(s) 177, 470
Ppu21I YACGTR 1 cut(s) 90
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 222, 237
PspPI GGNCC 2 cut(s) 28, 235
PspPPI RGGWCCY 1 cut(s) 235
PstI CTGCAG 2 cut(s) 263, 473
PsuI RGATCY 2 cut(s) 136, 414
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 92, 201
SaqAI TTAA 2 cut(s) 330, 478
SatI GCNGC 2 cut(s) 176, 469
Sau3AI GATC 2 cut(s) 136, 414
Sau96I GGNCC 2 cut(s) 28, 235
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 1 cut(s) 225
SfcI CTRYAG 2 cut(s) 259, 469
Sfr274I CTCGAG 1 cut(s) 286
SinI GGWCC 2 cut(s) 28, 235
SlaI CTCGAG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 92, 201
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
Sse9I AATT 2 cut(s) 292, 360
SseBI AGGCCT 1 cut(s) 147
SsiI CCGC 1 cut(s) 218
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 4 cut(s) 92, 165, 254, 460
TaqI TCGA 3 cut(s) 60, 129, 287
TasI AATT 2 cut(s) 292, 360
Tru1I TTAA 2 cut(s) 330, 478
Tru9I TTAA 2 cut(s) 330, 478
TscAI CASTG 2 cut(s) 263, 372
TseI GCWGC 2 cut(s) 175, 468
TspDTI ATGAA 2 cut(s) 185, 444
TspRI CASTG 2 cut(s) 263, 372
VpaK11BI GGWCC 2 cut(s) 28, 235
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 440
XhoI CTCGAG 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.