MD10G1218300.v1.1

Class I heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016287
Physical Location & Seq
Reverse (-)
31639809 .. 31640195
387 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1218300.v1.1.491

Sequence Viewer

Length: 387 bp
ATGTCCATTTTATTGCAATCTCTCTTAGATCAGCCCAACTTTTTGGCACCCTTCAGAGTTTTCGATTCAGAAATATCCAACAACACCACATACATGGACTGGAAGGAGACGTCAAATGCTCACATCTTTGAAATAGATCTTCCAGGCCTTGCAAAAGAGGACGTGAAGCTTGAGCTGCAAGAAAACAAAGCGCTTCGTGTTACTGCAGAGAGAAAACTGGAGCCTGAAACGGAAGACCCCAAGCATGAGACGTGGCACTGTAAGGAAAGGACAAGTGGAAGCTTCTCGAGGAATTTTAGGTTGCCTGACAAAGCCAAGGTCGATGAGATTAAGGCTTCAATGCGTGACGGGGTGTTGGTAATTACAGTGCCTAGGAGGAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

129

Amino Acids

14.87

Weight (kDa)

5.94

Isoelectric Point (pI)

62.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 127 2.1e-23 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016287)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13900
malus_domestica MD05G1240300.v1.1 MD10G1218300.v1.1
prunus_persica Prupe.4G125800_v2.0.a1
pyrus_communis pycom05g21630
rosa_chinensis RchiOBHm_Chr5g0023111
rosa_laevigata RLG00000032699
rosa_roxburghii Rroxscaffold_1G00056240
rosa_rugosa Rorug05G0077200
rosa_samantha Rh5AG163300 Rh5BG162600 Rh5CG177500 Rh5DG163900
rosa_wichuraiana Rw5G014730 Rw5G015000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 46
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 37
AcyI GRCGYC 1 cut(s) 110
AfeI AGCGCT 1 cut(s) 192
AgsI TTSAA 2 cut(s) 131, 339
AjiI CACGTC 2 cut(s) 163, 252
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 3 cut(s) 169, 175, 282
AluI AGCT 3 cut(s) 169, 175, 282
Alw26I GTCTC 2 cut(s) 101, 242
Ama87I CYCGRG 1 cut(s) 286
Aor51HI AGCGCT 1 cut(s) 192
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 175
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 286
AvrII CCTAGG 1 cut(s) 371
BanI GGYRCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 101, 242
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 204
BfoI RGCGCY 1 cut(s) 194
BglII AGATCT 1 cut(s) 136
BisI GCNGC 1 cut(s) 176
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 144
BmeT110I CYCGRG 1 cut(s) 286
BmgBI CACGTC 2 cut(s) 163, 252
BmiI GGNNCC 2 cut(s) 48, 222
BmrFI CCNGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 315, 371
Bse1I ACTGG 2 cut(s) 104, 222
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 315, 371
BseNI ACTGG 2 cut(s) 104, 222
BseXI GCAGC 1 cut(s) 162
BshFI GGCC 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 286
BsmAI GTCTC 2 cut(s) 101, 242
BsmBI CGTCTC 2 cut(s) 101, 242
BsnI GGCC 1 cut(s) 147
BsoBI CYCGRG 1 cut(s) 286
Bsp143I GATC 2 cut(s) 28, 136
BspANI GGCC 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 48, 222
BspMAI CTGCAG 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 46
BsrI ACTGG 2 cut(s) 104, 222
BssECI CCNNGG 2 cut(s) 315, 371
BssMI GATC 2 cut(s) 28, 136
BssNI GRCGYC 1 cut(s) 110
BssT1I CCWWGG 2 cut(s) 315, 371
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 260, 367
BstACI GRCGYC 1 cut(s) 110
BstDEI CTNAG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 2 cut(s) 31, 139
BstMAI GTCTC 2 cut(s) 101, 242
BstMBI GATC 2 cut(s) 28, 136
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 136
BstXI CCANNNNNNTGG 2 cut(s) 43, 94
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 1 cut(s) 147
BtrI CACGTC 2 cut(s) 163, 252
BtsIMutI CAGTG 2 cut(s) 256, 372
CfoI GCGC 1 cut(s) 193
CviAII CATG 2 cut(s) 94, 245
CviJI RGCY 8 cut(s) 34, 147, 169, 175, 223, 282, 314, 335
CviKI_1 RGCY 8 cut(s) 34, 147, 169, 175, 223, 282, 314, 335
DdeI CTNAG 1 cut(s) 25
DpnI GATC 2 cut(s) 30, 138
DpnII GATC 2 cut(s) 28, 136
Eco130I CCWWGG 2 cut(s) 315, 371
Eco147I AGGCCT 1 cut(s) 147
Eco47III AGCGCT 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 2 cut(s) 315, 371
ErhI CCWWGG 2 cut(s) 315, 371
Esp3I CGTCTC 2 cut(s) 101, 242
FaeI CATG 2 cut(s) 97, 248
FaiI YATR 3 cut(s) 91, 95, 246
FatI CATG 2 cut(s) 93, 244
Fnu4HI GCNGC 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 176
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 192
GluI GCNGC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 1 cut(s) 147
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 2 cut(s) 97, 248
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HindIII AAGCTT 2 cut(s) 167, 280
HinfI GANTC 1 cut(s) 65
Hpy188I TCNGA 2 cut(s) 56, 70
Hpy188III TCNNGA 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 61, 97
HpyCH4III ACNGT 2 cut(s) 260, 367
HpyCH4IV ACGT 3 cut(s) 110, 162, 251
HpyCH4V TGCA 4 cut(s) 16, 152, 178, 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 25
HpySE526I ACGT 3 cut(s) 110, 162, 251
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 2 cut(s) 97, 248
HspAI GCGC 1 cut(s) 191
Kzo9I GATC 2 cut(s) 28, 136
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 6 cut(s) 85, 129, 156, 203, 237, 318
Lsp1109I GCAGC 1 cut(s) 162
MaeI CTAG 1 cut(s) 372
MaeII ACGT 3 cut(s) 110, 162, 251
MaeIII GTNAC 2 cut(s) 199, 344
MalI GATC 2 cut(s) 30, 138
MboI GATC 2 cut(s) 28, 136
MboII GAAGA 2 cut(s) 131, 245
MflI RGATCY 1 cut(s) 136
MluCI AATT 3 cut(s) 292, 360, 382
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 4 cut(s) 151, 282, 369, 372
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 175
NdeII GATC 2 cut(s) 28, 136
NlaIII CATG 2 cut(s) 97, 248
NlaIV GGNNCC 2 cut(s) 48, 222
NmuCI GTSAC 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 286
PceI AGGCCT 1 cut(s) 147
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 48, 222
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 2 cut(s) 28, 136
ScrFI CCNGG 1 cut(s) 144
SetI ASST 8 cut(s) 113, 165, 171, 177, 254, 284, 302, 321
SfcI CTRYAG 1 cut(s) 204
Sfr274I CTCGAG 1 cut(s) 286
SlaI CTCGAG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 170, 286
SmoI CTYRAG 2 cut(s) 170, 286
Sse9I AATT 3 cut(s) 292, 360, 382
SseBI AGGCCT 1 cut(s) 147
SspMI CTAG 1 cut(s) 372
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 2 cut(s) 315, 371
TaaI ACNGT 2 cut(s) 260, 367
TaiI ACGT 3 cut(s) 113, 165, 254
TaqI TCGA 3 cut(s) 63, 287, 321
TasI AATT 3 cut(s) 292, 360, 382
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 263, 372
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 2 cut(s) 263, 372
XapI RAATTY 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 286
XmaJI CCTAGG 1 cut(s) 371
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.